mRNA_M-pyrifera_M_contig81874.19159.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81874.19159.1
Unique NamemRNA_M-pyrifera_M_contig81874.19159.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A1Q5UHF2_9EURO (Splicing factor U2AF subunit n=5 Tax=Penicillium TaxID=5073 RepID=A0A1Q5UHF2_9EURO)

HSP 1 Score: 62.0 bits (149), Expect = 4.330e-8
Identity = 55/189 (29.10%), Postives = 96/189 (50.79%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDLSRCEVEEEGNGHARR--ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASAA---------------------TGARIAVRLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            I +  V  +L +FG+L    + ++ +    R  A  ++A+P+ T  AVEGL+G ++LG  ++ + R  +    AA                     TG R+ ++L++  T +++   DD E +LED+ +E  + GKV     +++ PR  G +      GV  +Y+ FE ++SAS+ +K L GRKF  R
Sbjct:  392 IPEEPVTMLLKSFGELKSFVLVKDASTEESRGIAFCEYADPTATTIAVEGLNG-MELGDRHLKVVRASIGTTQAAGLDMGVNAMSMFAKTTSQDMETG-RV-LQLLNMVTVDELLDNDDYEEILEDVQEECAKYGKVL----SLKIPRPTGGNRQAA--GVGKIYVKFETQESASSALKALAGRKFSDR 571          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A0C3HNY5_9PEZI (Splicing factor U2AF subunit n=1 Tax=Oidiodendron maius Zn TaxID=913774 RepID=A0A0C3HNY5_9PEZI)

HSP 1 Score: 60.8 bits (146), Expect = 1.080e-7
Identity = 54/194 (27.84%), Postives = 98/194 (50.52%), Query Frame = 1
Query:    1 VAPLGPEIDDGAVEAVLSAFGDLSRCE-VEEEGNGHARR-ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRV---------------SEASAATGARI----AVRLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            ++ L P + D  V  +L AFG+L     V++ G   +R  A  ++A+P+ TD AVEGL+G +++G  ++ ++R  +               S  ++ T A +     ++L++  T E++   DD E + ED+ +E  + GKV      ++ PR  G    +   GV  +++ F+  +SA   ++ L GRKF  R
Sbjct:  377 ISNLPPYLTDEQVTELLLAFGELKAFVLVKDNGTEESRGIAFCEYADPTKTDIAVEGLNG-MEIGDKHLTVQRASIGLTQVASLEMSVNAMSMLASTTSAGLEDGRVLQLLNMVTAEELIDNDDYEEIQEDVREECDKYGKVLE----MKIPRPTGGSRQSN--GVGKIFVKFDTPESAGKALRALAGRKFADR 563          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A0F4YUB3_TALEM (Splicing factor U2AF subunit n=1 Tax=Rasamsonia emersonii CBS 393.64 TaxID=1408163 RepID=A0A0F4YUB3_TALEM)

HSP 1 Score: 59.3 bits (142), Expect = 3.630e-7
Identity = 56/188 (29.79%), Postives = 93/188 (49.47%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDL-SRCEVEEEGNGHARR-ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASAATGARIAV--------------------RLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            I +  V  +L +FG+L S   V++ G   +R  A  ++A+PS T  AVEGL+G ++LG  ++ + R  +  A+ A G  + V                    +L++  T E++   DD E + ED+ DE  + G+V      ++ PR  G    +   GV  +Y+ F+  +SA+  +K L GRKF  R
Sbjct:  353 IPEEPVTMLLKSFGELKSFVLVKDSGTEESRGIAFCEYADPSATSIAVEGLNG-MELGDKHLKVTRASIG-ATQAAGLDMGVNAMSMFAKTTSQDLETSRVLQLLNMVTPEELMNNDDYEEIREDVQDECNKYGQVLE----LKIPRPTGGSRQS--PGVGKIYVKFDSVESATKALKALAGRKFSDR 532          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A507QLT3_MONPU (Splicing factor U2AF subunit n=1 Tax=Monascus purpureus TaxID=5098 RepID=A0A507QLT3_MONPU)

HSP 1 Score: 59.3 bits (142), Expect = 3.640e-7
Identity = 57/195 (29.23%), Postives = 90/195 (46.15%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDLSRCEVEEEGNGHARR--ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASA----ATGARIAV--------------------RLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTG---MEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            I +  V  +L +FG+L    + ++      R  A  ++A+PS T  AVEGL+G ++LG      R L+V+ AS     ATG  + V                    +L++  T E++   DD E +++D+ DE  + G +           L  P P TG     GV  +Y+ F+  +SA+  +K L GRKF  R
Sbjct:  360 IPEEPVTMLLKSFGELKSFVLVKDNATEESRGIAFCEYADPSATSIAVEGLNG-MELGD-----RHLKVTRASIGITQATGLDMGVNAMSMFAKTTSQDLETSRVLQLLNMVTPEELMDNDDYEEIMDDVRDECSKYGTILE---------LKIPRPTTGGRQSPGVGKIYVKFDSVKSATEALKALAGRKFSDR 539          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A232LT16_9EURO (Uncharacterized protein n=1 Tax=Elaphomyces granulatus TaxID=519963 RepID=A0A232LT16_9EURO)

HSP 1 Score: 57.8 bits (138), Expect = 1.250e-6
Identity = 56/189 (29.63%), Postives = 95/189 (50.26%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDL-SRCEVEEEGNGHARR-ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVS-EASAATGARIAV--------------------RLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            I +  V+ +L +FG+L S   V++ G   +R  A  ++A+PS T  AVEGL+G   +  G+  LR +R S  A+ A G  + V                    +L++  T E++  +DD + + +D+ +E  + G+V      ++ PR  G    +   GV  +Y+ F+D +SA+  +K L GRKF  R
Sbjct:  402 IPEEPVKMLLISFGELKSFVLVKDLGTDESRGVAFCEYADPSATTIAVEGLNG---MELGDKHLRVIRASIGATQAAGLDMGVNAMSMFAKTTSQDLETTRVLQLLNMVTPEELMNSDDYDEICDDVQEECSKYGEVLD----LKIPRPTGGSRQSA--GVGKIYVKFDDVESATKALKALAGRKFSDR 581          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: W4JZH6_HETIT (Splicing factor U2AF subunit n=1 Tax=Heterobasidion irregulare (strain TC 32-1) TaxID=747525 RepID=W4JZH6_HETIT)

HSP 1 Score: 55.1 bits (131), Expect = 9.940e-6
Identity = 56/208 (26.92%), Postives = 92/208 (44.23%), Query Frame = 1
Query:    1 VAPLGPEIDDGAVEAVLSAFGDLSRCE-VEEEGNGHARR-ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASAATG--------------------------ARIAVRLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTG-------MEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            V  L   +++  V  +L +FG+L     V E GNG ++  A  ++ +PS+TD A++ LSG ++LG   ++++R  V   +   G                          ARI + +++  T +D+    +   +L+DI +E    G        VED R+  P    G         GV  VY+ + D   A+A +K L GR F GR
Sbjct:  228 VGGLPSYLNEEQVMELLKSFGELRAFNLVRENGNGASKGFAFFEYNDPSVTDVAIQSLSG-MELGDRYLVVQRASVGAKAGQPGIPNLPYDQFPEIPRPILPAGDLSTSDARILL-MLNMVTPDDLVDDQEYADILDDIREEVGNYG-------TVEDVRIPRPGAGIGDAQRADEASGVGRVYVKYTDSNGAAAAMKALAGRAFAGR 426          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A1L9SII9_9EURO (Splicing factor U2AF subunit n=1 Tax=Penicilliopsis zonata CBS 506.65 TaxID=1073090 RepID=A0A1L9SII9_9EURO)

HSP 1 Score: 55.1 bits (131), Expect = 1.060e-5
Identity = 53/188 (28.19%), Postives = 92/188 (48.94%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDLSRCEVEEEGNGHARR--ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASAATGARIAV--------------------RLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            I +  V  +L +FG+L    + ++ +    R  A  ++A+PS T  AVEGL+G ++LG  ++ + R  V  A+ ATG  + V                    +L++  T E++   DD E + +D+ DE  + G+V      ++ PR  G    +   GV  +++ F+  +SA+  +K L GRKF  R
Sbjct:  388 IPEEPVTMLLKSFGELKSFILVKDRSTEESRGIAFCEYADPSATAIAVEGLNG-MELGDRHLKIVRASVG-ATQATGLDMGVNAMSMFAKTTSNDLETTRVLQLLNMVTPEELLDNDDYEEICDDVRDECSKYGQVVD----LKVPRPTGGSRQSA--GVGKIFVRFDSVESATNALKALAGRKFSDR 567          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A6G1I6K8_9PEZI (Splicing factor U2AF subunit n=1 Tax=Trichodelitschia bisporula TaxID=703511 RepID=A0A6G1I6K8_9PEZI)

HSP 1 Score: 54.7 bits (130), Expect = 1.410e-5
Identity = 50/188 (26.60%), Postives = 91/188 (48.40%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDLSRCEVEEEGNGHARR--ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASAATGARIAV--------------------RLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            + D  V  +L AFG+L    + ++ +    R  A  ++A+P++T+ AVEGL+G ++LG  ++ ++R  +    AA G  ++V                    +L++  T E++   D+ E + +D+  E ++ G V      V+ PR  G   + G   V  +Y+ F   +SA   ++ L GRKF  R
Sbjct:  360 LTDNQVTELLVAFGELKAFVLVKDQSSEQSRGIAFCEYADPAVTEIAVEGLNG-MELGDDHLQVKRASIGVQQAA-GLEMSVNAMSMLAGTTATDLEQGRVIQLMNMVTAEELMDNDEYEEICDDVKTECEKFGPVLE----VKIPRPSGARTNPG---VGKIYVKFAAPESAQKAMRGLAGRKFADR 538          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: D8UJ72_VOLCA (Uncharacterized protein n=1 Tax=Volvox carteri f. nagariensis TaxID=3068 RepID=D8UJ72_VOLCA)

HSP 1 Score: 54.3 bits (129), Expect = 1.450e-5
Identity = 52/168 (30.95%), Postives = 80/168 (47.62%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDLSRCEVEEEGNGHARRA--LVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASAATGARIAVRLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLH---GPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKF 510
            + +  V  +L AFG L    +  + +  A +     ++A+P+ITD A++GLS  I +G   + +RR   +  ++AT   +  +   A    D G   D   L+ED+  E  + GK    +  VE PR     GPDP     GV  VY+ +ED + A      L GRKF
Sbjct:  118 LSEEQVRQILQAFGPLRAFNLVTDRDTGASKGYGFCEYADPNITDVAIQGLSALI-VGGKPLTVRRANTAGEASATLQTLIQQQQAALDLVDDGEYMD---LMEDVTQEVGKYGK----LVGVEIPRPPPDGGPDP----PGVGFVYLCYEDPRGAERAQVALNGRKF 273          
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Match: A0A017SNU8_9EURO (Splicing factor U2AF subunit n=4 Tax=Aspergillus TaxID=5052 RepID=A0A017SNU8_9EURO)

HSP 1 Score: 54.3 bits (129), Expect = 1.930e-5
Identity = 53/192 (27.60%), Postives = 91/192 (47.40%), Query Frame = 1
Query:   22 IDDGAVEAVLSAFGDLSRCEVEEEGNGHARR--ALVQFANPSITDTAVEGLSGEIDLGAGNVILRRLRVSEASA----ATGARIAV--------------------RLIHAATTEDIGAADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYIVFEDEQSASACVKLLGGRKFEGR 519
            I +  V  +L +FG+L    + ++G+    R  A  ++A+P+ T  AVEGL+G ++LG      R L+V  AS     A+G  + V                    +L++  T E++   DD E + +D+ +E  + G+    I  V+ PR  G    +   GV  +++ F+  +S +  +K L GRKF  R
Sbjct:  374 IPEEPVTMLLKSFGELKSFVLVKDGSTEESRGIAFCEYADPTATSIAVEGLNG-MELGD-----RHLKVVRASIGITQASGLDMGVNAMSMFAKTTSQDLETSRVLQLLNMVTPEELMDNDDYEEICDDVQEECSKYGQ----ILGVKIPRPSGGSRQSA--GVGKIFVKFDTVESTTNALKALAGRKFSDR 553          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81874.19159.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 17
Match NameE-valueIdentityDescription
A0A1Q5UHF2_9EURO4.330e-829.10Splicing factor U2AF subunit n=5 Tax=Penicillium T... [more]
A0A0C3HNY5_9PEZI1.080e-727.84Splicing factor U2AF subunit n=1 Tax=Oidiodendron ... [more]
A0A0F4YUB3_TALEM3.630e-729.79Splicing factor U2AF subunit n=1 Tax=Rasamsonia em... [more]
A0A507QLT3_MONPU3.640e-729.23Splicing factor U2AF subunit n=1 Tax=Monascus purp... [more]
A0A232LT16_9EURO1.250e-629.63Uncharacterized protein n=1 Tax=Elaphomyces granul... [more]
W4JZH6_HETIT9.940e-626.92Splicing factor U2AF subunit n=1 Tax=Heterobasidio... [more]
A0A1L9SII9_9EURO1.060e-528.19Splicing factor U2AF subunit n=1 Tax=Penicilliopsi... [more]
A0A6G1I6K8_9PEZI1.410e-526.60Splicing factor U2AF subunit n=1 Tax=Trichodelitsc... [more]
D8UJ72_VOLCA1.450e-530.95Uncharacterized protein n=1 Tax=Volvox carteri f. ... [more]
A0A017SNU8_9EURO1.930e-527.60Splicing factor U2AF subunit n=4 Tax=Aspergillus T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81874contigM-pyrifera_M_contig81874:269..787 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score66.2
Seed ortholog evalue1.7e-08
Seed eggNOG ortholog121759.XP_010763103.1
KEGG koko:K12837
KEGG Pathwayko03040,map03040
Hectar predicted targeting categoryother localisation
GOsGO:0000243,GO:0000245,GO:0000348,GO:0000375,GO:0000377,GO:0000398,GO:0003674,GO:0003676,GO:0003723,GO:0003727,GO:0003729,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005681,GO:0005684,GO:0006139,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0008187,GO:0008380,GO:0009987,GO:0010467,GO:0016043,GO:0016070,GO:0016071,GO:0016604,GO:0016607,GO:0022607,GO:0022613,GO:0022618,GO:0030628,GO:0031974,GO:0031981,GO:0032991,GO:0034622,GO:0034641,GO:0036002,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0045131,GO:0045292,GO:0046483,GO:0065003,GO:0070013,GO:0071004,GO:0071010,GO:0071704,GO:0071826,GO:0071840,GO:0089701,GO:0090304,GO:0097159,GO:1901360,GO:1901363,GO:1990904
EggNOG free text desc.U2 snRNP auxiliary factor large subunit
EggNOG OGs20F74@147545,38BQ8@33154,3B1N4@33183,3FIV6@34383,3NUP9@4751,3QK9U@4890,KOG0120@1,KOG0120@2759
COG Functional cat.J
Best tax levelOnygenales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03041
Exons1
Model size519
Cds size519
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816726.0721338-CDS-M-pyrifera_M_contig81874:268..7871622816726.0721338-CDS-M-pyrifera_M_contig81874:268..787Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81874 269..787 -
1692278142.7517107-CDS-M-pyrifera_M_contig81874:268..7871692278142.7517107-CDS-M-pyrifera_M_contig81874:268..787Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81874 269..787 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81874.19159.1prot_M-pyrifera_M_contig81874.19159.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81874 269..787 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81874.19159.1

>prot_M-pyrifera_M_contig81874.19159.1 ID=prot_M-pyrifera_M_contig81874.19159.1|Name=mRNA_M-pyrifera_M_contig81874.19159.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=173bp
VAPLGPEIDDGAVEAVLSAFGDLSRCEVEEEGNGHARRALVQFANPSITD
TAVEGLSGEIDLGAGNVILRRLRVSEASAATGARIAVRLIHAATTEDIGA
ADDVEALLEDIADEAQRAGKVARCIAAVEDPRLHGPDPHTGMEGVVPVYI
VFEDEQSASACVKLLGGRKFEGR
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mRNA from alignment at M-pyrifera_M_contig81874:269..787-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81874.19159.1 ID=mRNA_M-pyrifera_M_contig81874.19159.1|Name=mRNA_M-pyrifera_M_contig81874.19159.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=519bp|location=Sequence derived from alignment at M-pyrifera_M_contig81874:269..787- (Macrocystis pyrifera P11B4 male)
GTTGCACCACTCGGACCAGAGATTGATGATGGTGCGGTAGAAGCTGTATT GAGTGCGTTTGGTGACTTGTCAAGGTGTGAAGTGGAAGAAGAAGGCAATG GACATGCAAGAAGAGCATTGGTGCAATTTGCAAATCCTTCCATCACAGAT ACTGCAGTGGAAGGCTTGAGCGGAGAAATCGATCTTGGTGCTGGAAATGT AATTTTGCGTCGTCTTCGAGTGTCTGAAGCATCTGCAGCTACAGGTGCAC GAATTGCTGTGCGATTAATTCATGCAGCAACTACAGAAGACATTGGTGCA GCAGATGATGTGGAAGCACTCTTGGAAGACATTGCAGATGAAGCACAGCG AGCCGGAAAAGTTGCTCGTTGTATTGCTGCTGTAGAAGATCCACGATTAC ATGGTCCTGATCCACATACTGGGATGGAAGGGGTGGTTCCAGTCTACATT GTGTTCGAAGATGAACAATCAGCAAGTGCATGTGTGAAACTTCTTGGTGG GAGAAAGTTTGAAGGGAGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81874:269..787-

>mRNA_M-pyrifera_M_contig81874.19159.1 ID=mRNA_M-pyrifera_M_contig81874.19159.1|Name=mRNA_M-pyrifera_M_contig81874.19159.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1038bp|location=Sequence derived from alignment at M-pyrifera_M_contig81874:269..787- (Macrocystis pyrifera P11B4 male)
GTTGCACCACTCGGACCAGAGATTGATGATGGTGCGGTAGAAGCTGTATT
GAGTGCGTTTGGTGACTTGTCAAGGTGTGAAGTGGAAGAAGAAGGCAATG
GACATGCAAGAAGAGCATTGGTGCAATTTGCAAATCCTTCCATCACAGAT
ACTGCAGTGGAAGGCTTGAGCGGAGAAATCGATCTTGGTGCTGGAAATGT
AATTTTGCGTCGTCTTCGAGTGTCTGAAGCATCTGCAGCTACAGGTGCAC
GAATTGCTGTGCGATTAATTCATGCAGCAACTACAGAAGACATTGGTGCA
GCAGATGATGTGGAAGCACTCTTGGAAGACATTGCAGATGAAGCACAGCG
AGCCGGAAAAGTTGCTCGTTGTATTGCTGCTGTAGAAGATCCACGATTAC
ATGGTCCTGATCCACATACTGGGATGGAAGGGGTGGTTCCAGTCTACATT
GTGTTCGAAGATGAACAATCAGCAAGTGCATGTGTGAAACTTCTTGGTGG
GAGAAAGTTTGAAGGGAGAGTTGCACCACTCGGACCAGAGATTGATGATG
GTGCGGTAGAAGCTGTATTGAGTGCGTTTGGTGACTTGTCAAGGTGTGAA
GTGGAAGAAGAAGGCAATGGACATGCAAGAAGAGCATTGGTGCAATTTGC
AAATCCTTCCATCACAGATACTGCAGTGGAAGGCTTGAGCGGAGAAATCG
ATCTTGGTGCTGGAAATGTAATTTTGCGTCGTCTTCGAGTGTCTGAAGCA
TCTGCAGCTACAGGTGCACGAATTGCTGTGCGATTAATTCATGCAGCAAC
TACAGAAGACATTGGTGCAGCAGATGATGTGGAAGCACTCTTGGAAGACA
TTGCAGATGAAGCACAGCGAGCCGGAAAAGTTGCTCGTTGTATTGCTGCT
GTAGAAGATCCACGATTACATGGTCCTGATCCACATACTGGGATGGAAGG
GGTGGTTCCAGTCTACATTGTGTTCGAAGATGAACAATCAGCAAGTGCAT
GTGTGAAACTTCTTGGTGGGAGAAAGTTTGAAGGGAGA
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