mRNA_M-pyrifera_M_contig81703.19126.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81703.19126.1
Unique NamemRNA_M-pyrifera_M_contig81703.19126.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A7Y5W246_9BACT (DUF11 domain-containing protein n=1 Tax=Pirellulales bacterium TaxID=2715253 RepID=A0A7Y5W246_9BACT)

HSP 1 Score: 139 bits (350), Expect = 4.270e-35
Identity = 71/154 (46.10%), Postives = 99/154 (64.29%), Query Frame = 1
Query:  130 NVLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRD--PQVPEKRGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            NVL  R+SP+LSVET+GP R+ +G+++ Y V + NAG V A+ V V+V +P   DVVG D S G T    P       ++W +  L A  +  L ++I+PR+SRPFDL VQW  +   +QA +EVQEPKL+M L GP EV Y +P  Y+L ++N
Sbjct:   68 NVLFARQSPILSVETLGPKRIAVGKQSTYEVSIQNAGQVAADQVVVTVDLPGWADVVGTDASSGSTESIPPSADAPGRLLWKVGHLDAKGSERLVLRILPRESRPFDLKVQWDDTPTESQAAIEVQEPKLAMELQGPREVVYAKPEVYRLEITN 221          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A7C3WJ92_9BACT (Uncharacterized protein (Fragment) n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7C3WJ92_9BACT)

HSP 1 Score: 137 bits (346), Expect = 5.970e-35
Identity = 67/153 (43.79%), Postives = 104/153 (67.97%), Query Frame = 1
Query:  130 NVLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRG-IIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            ++L+  +SP+LS++T GP R+ +G+EA Y VV+ N+GTV AN + V+V +P   +V+  + S G T+ P   +K G  +W + +L      +L ++IVPR+SRPF+LGV+W+++ Q  QA +EVQEPKL + L GP EV +GQ   YKL ++N
Sbjct:  121 SLLMAPQSPILSIQTHGPRRITVGKEAPYEVVIQNSGTVAANDLVVTVDLPPWAEVLSAEASVGSTQAPGPTDKAGQFLWRIGRLDPRAQEKLVLRIVPRESRPFELGVRWSFTPQRLQAAIEVQEPKLGLQLEGPREVVHGQRQIYKLIMTN 273          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A7X7KH48_9BACT (DUF11 domain-containing protein n=1 Tax=Pirellulaceae bacterium TaxID=2699754 RepID=A0A7X7KH48_9BACT)

HSP 1 Score: 138 bits (348), Expect = 1.950e-34
Identity = 71/152 (46.71%), Postives = 99/152 (65.13%), Query Frame = 1
Query:  133 VLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRGII-WMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            VLI RKSP L VET+GP  +++GRE+ Y V+++N+G   A G+ V VS+PD  +VV  +   G  +  Q   +  I+ W +  L A + AEL +KIVPR  RPFDL + W Y   A+QA +EVQEPKL++ L GP+EV YG+   ++L LSN
Sbjct:  293 VLIARKSPNLGVETLGPRTIMVGRESTYKVIVANSGDAAAEGLAVFVSLPDWAEVVAVEAGAGAAQPEQDERQVKIVRWKVGDLAAKDRAELSLKIVPRQPRPFDLEISWNYKPTASQAAIEVQEPKLALKLVGPNEVFYGKKELFQLILSN 444          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A847YG10_9BACT (DUF11 domain-containing protein n=1 Tax=Thermogutta sp. TaxID=1962930 RepID=A0A847YG10_9BACT)

HSP 1 Score: 138 bits (348), Expect = 2.140e-34
Identity = 71/188 (37.77%), Postives = 110/188 (58.51%), Query Frame = 1
Query:   22 SAMEEHDNIVSPQTPAPRIADRRSNSNASSTSTSARNVLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            +A+  +D  +    P  R+A   +   A++  ++   VLI R +P+L+VETIGP R+ +GRE  YT+ L N G   A  V V V +P   D+ G +P+ G          R + W +++LP G    LD++I+PR+S+PFDL VQW ++   +QA +EVQEPKL + L GP+ + +GQ   Y+L + N
Sbjct:  358 TAVPANDETIRGPAPGSRVAAEPA---ATAAKSAGDRVLINRTTPLLNVETIGPRRITVGREGLYTLQLKNQGQTPARQVAVHVMIPHWADLNGAEPTGGTVSQQMEGAFRRLAWRIEELPVGAAESLDLRIIPRESKPFDLAVQWEFAPPPSQASIEVQEPKLELRLDGPETILFGQSQVYRLEVEN 542          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A3M1SM12_9BACT (DUF11 domain-containing protein (Fragment) n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M1SM12_9BACT)

HSP 1 Score: 130 bits (328), Expect = 7.480e-33
Identity = 64/158 (40.51%), Postives = 96/158 (60.76%), Query Frame = 1
Query:  112 TSTSARNVLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            T T+   VLI R+SP++ V T+GP R+ +G+E  Y +++ N G + A+ V VSV +P+  DV+  + + G T     P  R   W +  LP      L ++IVPR S+PFDL V+W +   AT+  +EVQE KL ++L GP E+ YG+ A Y+L L+N
Sbjct:  165 TDTTDEPVLIERQSPIIQVRTLGPRRISVGKEGIYQIIVENTGNLGADQVTVSVDLPEWADVLSAETTTGATNVDVRPGARTFTWKIPHLPEAGRELLKLRIVPRQSKPFDLAVKWDFVPLATETQIEVQEAKLQLNLEGPREILYGESAVYRLELAN 322          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A2E3REA7_9PLAN (Uncharacterized protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2E3REA7_9PLAN)

HSP 1 Score: 129 bits (325), Expect = 2.650e-31
Identity = 66/150 (44.00%), Postives = 96/150 (64.00%), Query Frame = 1
Query:  136 LITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            L+  K+P L+VET+GPS V+IG+EA + + L N G   A  V V ++ P G  +V   PS GQT      +++ + W L++L A ET  L ++IVPR S  FDLGV WT +  A+QA ++VQEPKL++ + GP E+ YG    Y+LT++N
Sbjct:  385 LLFTKNPQLTVETLGPSEVVIGKEATFKIRLRNLGDPVAESVLVRINTPLGAQIVAAQPSRGQTNSVAASKQKPLEWRLERLDAHETEFLTLRIVPRKSDAFDLGVTWTLAPIASQARIKVQEPKLNLEIVGPHEIDYGDTKIYRLTITN 534          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A3E0N002_9BACT (DUF11 domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3E0N002_9BACT)

HSP 1 Score: 129 bits (325), Expect = 2.680e-31
Identity = 65/156 (41.67%), Postives = 95/156 (60.90%), Query Frame = 1
Query:  124 ARNVLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEK--RGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            A ++L  +KSP+L VE IGP R+++G+E  Y V + N G V A+GV V V +P G DV G D   G  R     +   R + W L +L     ++L +++VPRDS PF++ V WT S+   + ++EVQEPKL + L GP EV +G    Y+LT++N
Sbjct:  396 ADSILFAQKSPLLRVEAIGPRRIVVGKEVTYRVRMQNLGDVAADGVAVQVQLPVGADVAGTDAGEGTARTIPADQSGPRRLEWQLGRLEGRSHSQLVIRLVPRDSSPFEMAVNWTSSATRGETLIEVQEPKLQLDLDGPREVVFGDSKVYRLTVAN 551          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A7X7ISC6_9BACT (DUF11 domain-containing protein n=1 Tax=Pirellulaceae bacterium TaxID=2699754 RepID=A0A7X7ISC6_9BACT)

HSP 1 Score: 129 bits (324), Expect = 3.610e-31
Identity = 63/153 (41.18%), Postives = 102/153 (66.67%), Query Frame = 1
Query:  133 VLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRG--IIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            VL+TR+SP+L+V+T+GPSR+ IG+ + Y V + N G V A+ V V++++P+  +VVG +P+ G    P     RG  ++W +  + A    ++ + IVPR SRP DL V+W+++   +QA++ V+EPKL+M L GP EV +G+P  +KL + N
Sbjct:  382 VLLTRRSPILNVQTLGPSRIAIGKRSDYEVQMQNLGAVAADDVVVTIALPEWAEVVGAEPTGGAIL-PDASASRGQQLLWKIGPMEASSRHKVVLGIVPRQSRPIDLAVRWSFTPIVSQAVIVVEEPKLAMRLGGPREVRFGEPQVFKLEIEN 533          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A1Z8MYK3_9PLAN (Uncharacterized protein n=1 Tax=Planctomycetaceae bacterium TMED10 TaxID=1986833 RepID=A0A1Z8MYK3_9PLAN)

HSP 1 Score: 129 bits (324), Expect = 3.650e-31
Identity = 67/150 (44.67%), Postives = 96/150 (64.00%), Query Frame = 1
Query:  136 LITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKRGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            L+  KSP LSVET+GPS+V+IG+EA + + L N G   A  V + ++ P G  +V   PS GQT      +++ + W LD+L A  T  L ++IVPR S  FDLGV WT +  A+QA ++VQEPKL++ + GP E+ YG    Y+LT++N
Sbjct:  419 LLFSKSPQLSVETLGPSQVVIGKEATFKIRLRNLGDPVAESVLLRINTPLGAQIVSAQPSRGQTNALAAAKQKPLEWRLDRLEAHATEFLTLRIVPRKSDSFDLGVTWTLAPIASQARIKVQEPKLNLEIIGPHEIDYGDTKLYRLTITN 568          
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Match: A0A3E0P8D0_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3E0P8D0_9BACT)

HSP 1 Score: 128 bits (322), Expect = 6.670e-31
Identity = 68/155 (43.87%), Postives = 97/155 (62.58%), Query Frame = 1
Query:  124 ARNVLITRKSPVLSVETIGPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQTRDPQVPEKR-GIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQATQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN 585
            A  VL++  SPV++V+  GP  VL+G+EA++ V  SN G   A  V +S+ +P   DV    P+ G  +     E R G++W LD L A     L +K+VPR S P DL + +T++ + +Q MVEVQEPKL+M+LSGP EV +G+   YKLT+SN
Sbjct:  353 AEGVLLSAASPVIAVQATGPRSVLVGKEAEFVVKASNTGAP-AKNVAISIHIPSYADVADARPTSGSAQPTSPGEARQGVVWTLDTLDAEAEETLVLKLVPRRSSPLDLALSYTFAPETSQTMVEVQEPKLAMTLSGPSEVPFGETRIYKLTISN 506          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81703.19126.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7Y5W246_9BACT4.270e-3546.10DUF11 domain-containing protein n=1 Tax=Pirellulal... [more]
A0A7C3WJ92_9BACT5.970e-3543.79Uncharacterized protein (Fragment) n=1 Tax=Plancto... [more]
A0A7X7KH48_9BACT1.950e-3446.71DUF11 domain-containing protein n=1 Tax=Pirellulac... [more]
A0A847YG10_9BACT2.140e-3437.77DUF11 domain-containing protein n=1 Tax=Thermogutt... [more]
A0A3M1SM12_9BACT7.480e-3340.51DUF11 domain-containing protein (Fragment) n=1 Tax... [more]
A0A2E3REA7_9PLAN2.650e-3144.00Uncharacterized protein n=1 Tax=Planctomycetaceae ... [more]
A0A3E0N002_9BACT2.680e-3141.67DUF11 domain-containing protein n=1 Tax=Planctomyc... [more]
A0A7X7ISC6_9BACT3.610e-3141.18DUF11 domain-containing protein n=1 Tax=Pirellulac... [more]
A0A1Z8MYK3_9PLAN3.650e-3144.67Uncharacterized protein n=1 Tax=Planctomycetaceae ... [more]
A0A3E0P8D0_9BACT6.670e-3143.87Uncharacterized protein n=1 Tax=Planctomycetes bac... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81703contigM-pyrifera_M_contig81703:342..926 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score105.9
Seed ortholog evalue2e-20
Seed eggNOG ortholog595460.RRSWK_05873
KEGG koko:K08086
Hectar predicted targeting categoryother localisation
EggNOG free text desc.60 kDa outer membrane protein, serovars L1 L2 L3
EggNOG OGs2IZ29@203682,COG1361@1,COG1361@2,COG3170@1,COG3170@2
COG Functional cat.M
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons1
Model size585
Cds size558
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816724.5920773-UTR-M-pyrifera_M_contig81703:341..3681622816724.5920773-UTR-M-pyrifera_M_contig81703:341..368Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig81703 342..368 +
1692278141.8966756-UTR-M-pyrifera_M_contig81703:341..3681692278141.8966756-UTR-M-pyrifera_M_contig81703:341..368Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig81703 342..368 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816724.6031399-CDS-M-pyrifera_M_contig81703:368..9261622816724.6031399-CDS-M-pyrifera_M_contig81703:368..926Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81703 369..926 +
1692278141.9134445-CDS-M-pyrifera_M_contig81703:368..9261692278141.9134445-CDS-M-pyrifera_M_contig81703:368..926Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81703 369..926 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81703.19126.1prot_M-pyrifera_M_contig81703.19126.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81703 369..926 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81703.19126.1

>prot_M-pyrifera_M_contig81703.19126.1 ID=prot_M-pyrifera_M_contig81703.19126.1|Name=mRNA_M-pyrifera_M_contig81703.19126.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=186bp
MEEHDNIVSPQTPAPRIADRRSNSNASSTSTSARNVLITRKSPVLSVETI
GPSRVLIGREAKYTVVLSNAGTVNANGVQVSVSVPDGCDVVGFDPSFGQT
RDPQVPEKRGIIWMLDQLPAGETAELDVKIVPRDSRPFDLGVQWTYSSQA
TQAMVEVQEPKLSMSLSGPDEVAYGQPATYKLTLSN
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mRNA from alignment at M-pyrifera_M_contig81703:342..926+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81703.19126.1 ID=mRNA_M-pyrifera_M_contig81703.19126.1|Name=mRNA_M-pyrifera_M_contig81703.19126.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=585bp|location=Sequence derived from alignment at M-pyrifera_M_contig81703:342..926+ (Macrocystis pyrifera P11B4 male)
ACGATCGAACCCAAGCCAGATTCGGCAATGGAAGAGCACGACAACATCGT CTCGCCTCAGACTCCCGCGCCGCGAATTGCCGACCGCCGCTCGAATTCAA ACGCGAGCTCCACTTCCACGTCAGCCCGCAATGTGCTGATCACCCGCAAG AGCCCGGTGCTTTCCGTCGAAACCATCGGCCCCAGCCGAGTGTTGATTGG TCGTGAAGCGAAGTACACCGTTGTCTTGAGCAATGCCGGCACGGTGAACG CCAACGGGGTTCAGGTCTCGGTTTCCGTTCCAGATGGTTGTGACGTGGTT GGCTTCGATCCATCGTTCGGACAAACACGCGACCCACAGGTGCCGGAAAA ACGGGGCATCATTTGGATGCTCGATCAACTGCCCGCCGGTGAAACGGCTG AATTGGACGTGAAGATCGTGCCGCGAGACAGTCGCCCCTTCGATTTGGGC GTGCAATGGACCTACTCCTCGCAAGCCACGCAGGCCATGGTTGAAGTGCA AGAACCAAAGCTGTCCATGAGTCTTTCAGGACCCGATGAAGTTGCCTACG GGCAACCAGCCACTTACAAACTTACCCTCTCGAAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81703:342..926+

>mRNA_M-pyrifera_M_contig81703.19126.1 ID=mRNA_M-pyrifera_M_contig81703.19126.1|Name=mRNA_M-pyrifera_M_contig81703.19126.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1116bp|location=Sequence derived from alignment at M-pyrifera_M_contig81703:342..926+ (Macrocystis pyrifera P11B4 male)
ATGGAAGAGCACGACAACATCGTCTCGCCTCAGACTCCCGCGCCGCGAAT
TGCCGACCGCCGCTCGAATTCAAACGCGAGCTCCACTTCCACGTCAGCCC
GCAATGTGCTGATCACCCGCAAGAGCCCGGTGCTTTCCGTCGAAACCATC
GGCCCCAGCCGAGTGTTGATTGGTCGTGAAGCGAAGTACACCGTTGTCTT
GAGCAATGCCGGCACGGTGAACGCCAACGGGGTTCAGGTCTCGGTTTCCG
TTCCAGATGGTTGTGACGTGGTTGGCTTCGATCCATCGTTCGGACAAACA
CGCGACCCACAGGTGCCGGAAAAACGGGGCATCATTTGGATGCTCGATCA
ACTGCCCGCCGGTGAAACGGCTGAATTGGACGTGAAGATCGTGCCGCGAG
ACAGTCGCCCCTTCGATTTGGGCGTGCAATGGACCTACTCCTCGCAAGCC
ACGCAGGCCATGGTTGAAGTGCAAGAACCAAAGCTGTCCATGAGTCTTTC
AGGACCCGATGAAGTTGCCTACGGGCAACCAGCCACTTACAAACTTACCC
TCTCGAACATGGAAGAGCACGACAACATCGTCTCGCCTCAGACTCCCGCG
CCGCGAATTGCCGACCGCCGCTCGAATTCAAACGCGAGCTCCACTTCCAC
GTCAGCCCGCAATGTGCTGATCACCCGCAAGAGCCCGGTGCTTTCCGTCG
AAACCATCGGCCCCAGCCGAGTGTTGATTGGTCGTGAAGCGAAGTACACC
GTTGTCTTGAGCAATGCCGGCACGGTGAACGCCAACGGGGTTCAGGTCTC
GGTTTCCGTTCCAGATGGTTGTGACGTGGTTGGCTTCGATCCATCGTTCG
GACAAACACGCGACCCACAGGTGCCGGAAAAACGGGGCATCATTTGGATG
CTCGATCAACTGCCCGCCGGTGAAACGGCTGAATTGGACGTGAAGATCGT
GCCGCGAGACAGTCGCCCCTTCGATTTGGGCGTGCAATGGACCTACTCCT
CGCAAGCCACGCAGGCCATGGTTGAAGTGCAAGAACCAAAGCTGTCCATG
AGTCTTTCAGGACCCGATGAAGTTGCCTACGGGCAACCAGCCACTTACAA
ACTTACCCTCTCGAAC
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