mRNA_M-pyrifera_M_contig81448.19055.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81448.19055.1
Unique NamemRNA_M-pyrifera_M_contig81448.19055.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: UPI002025AC95 (DUF4214 domain-containing protein n=1 Tax=Rhodovastum atsumiense TaxID=504468 RepID=UPI002025AC95)

HSP 1 Score: 65.9 bits (159), Expect = 2.510e-9
Identity = 60/197 (30.46%), Postives = 83/197 (42.13%), Query Frame = 1
Query:   25 TASYFVAGLYETLLGRTADTGSLLGHVDRLMSGDPAAAVIETFWKSEEHRA--------LQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPS--VYA--------AEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPE---RYNVLLDR-----YYNDFLGRPLDP 537
            T   FVA LY++LLGRTAD   +    + L    P   V   F  S E+ A          +   Y +VL R   AD +  W+  LASG S  D+  + ++S E      ++ S  ++A         E+Y+  L REA    +  W   L  G+   D   A  NS E   R+    D       Y D LGR +DP
Sbjct:  565 TTPAFVARLYQSLLGRTADANGMAVWSNAL-GQQPPGTVTNAFINSAEYAARHGTQSDSTSIAGLYHDVLGRDGEADGMQTWIGALASGISRGDVAAAFVSSGEAQQHWADLTSQGLFAYDLDAEVVREVYKTGLGREAEAGAIGTWGNALHQGMSLTDFCTAISNSGEFQGRHAAQSDSAYVASLYQDGLGRTIDP 760          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: A0A1J5QHG7_9ZZZZ (Extracellular serine proteinase n=1 Tax=mine drainage metagenome TaxID=410659 RepID=A0A1J5QHG7_9ZZZZ)

HSP 1 Score: 61.2 bits (147), Expect = 9.600e-8
Identity = 27/67 (40.30%), Postives = 37/67 (55.22%), Query Frame = 1
Query:  337 PSVYAAEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPERYNVLLDRYYNDFLGRPLDP 537
            P  +   +Y  +L+R A P EV +W G+L  G   + VA  FL S E    ++D YY D LGR +DP
Sbjct:  593 PDSWVRSLYATVLNRTAGPSEVAYWVGRLNGGFSRSAVAYGFLLSTEHLTSVIDGYYQDLLGRGIDP 659          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: A0A512JB58_9HYPH (Uncharacterized protein n=1 Tax=Methylobacterium oxalidis TaxID=944322 RepID=A0A512JB58_9HYPH)

HSP 1 Score: 60.1 bits (144), Expect = 2.300e-7
Identity = 55/182 (30.22%), Postives = 79/182 (43.41%), Query Frame = 1
Query:   13 ADRVTASYFVAGLYETLLGRTADTGSLLGHVDRLMS-GDPAAAVIETFWKSEEH-----------------RALQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPSV-YAAEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPERYNVLLDR 501
            A  + +S FV+ LY++ LGR AD+G L    D+L + G     V   F  S EH                 +A  V   Y  +L R P A  L  W  QL  G + +D+    L + E VS   ++ S  Y   +Y   L R A  +   +W   LE+G   AD+A     S E ++V L +
Sbjct:  385 AGGLGSSEFVSQLYQSTLGRLADSGGLAYWTDQLDNHGAQRIDVASGFVFSAEHLSSLQSVFDAGLFVPDKQAADVARLYYTMLGRAPDAGGLQYWADQLEHGGTISDLAKVFLGTPENVSKYGSMGSFGYVDALYVNALGRHADADGQAYWEDLLEHGTSRADLAVLLSESAEAHSVHLSQ 566          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: UPI001AAF2E53 (DUF4214 domain-containing protein n=3 Tax=unclassified Cellulomonas TaxID=2620175 RepID=UPI001AAF2E53)

HSP 1 Score: 60.1 bits (144), Expect = 2.440e-7
Identity = 43/175 (24.57%), Postives = 63/175 (36.00%), Query Frame = 1
Query:   13 ADRVTASYFVAGLYETLLGRTADTGSLLGHVDRLMSGDPAAAVIETFWKSEEHRALQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPSVYAAEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPERYNVLLDRYYNDFLGRPLDP 537
            A  V    +V  +Y  L GR  D   L      ++ G P   V      S E+R   +                          G + + ++   L S E      +  + +   +Y  +L R     EV FW  +L  G    DV+  FL S E  + ++D YY   LGR LDP
Sbjct:   37 AQEVAVERYVVRVYRDLFGRAPDPTGLGTWRAAILDGRPRVEVANAITSSHEYRTRLITGTXXXXXXXXXXXXXXXXXXXXXXXGWTVSHVSAGFLASDEHYQQAGSTRAGWVVRLYDDVLGRTPGQSEVDFWVRELARGASRWDVSLGFLLSWEHLSTVVDGYYEQLLGRHLDP 211          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: Q0FQV3_SALBH (Hemolysin-type calcium-binding region n=2 Tax=Salipiger TaxID=263377 RepID=Q0FQV3_SALBH)

HSP 1 Score: 59.3 bits (142), Expect = 4.730e-7
Identity = 44/133 (33.08%), Postives = 66/133 (49.62%), Query Frame = 1
Query:  196 YAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVS---------LLKNIPSVYAAEMYRLI---LHREAAPEEVQFWAGQLENGVPHADVANAFLNSPE---RYNVLLDR-----YYNDFLGRPLD 534
            Y  VL+R P A  LA+W++QLASG +  ++      SSE+++         +L  I + +A ++YRL    L RE     +  W+ +   G  + DVA+ F+NSPE    Y  L D       YN+ L R  D
Sbjct:  849 YNNVLDRDPDAGGLANWLSQLASGTTREEVVRGFSQSSEYIAQTNDSASAYVLAGIAAPFADDVYRLYEATLDREPDVVGLTNWSTERGMGTSYLDVASGFVNSPEFQATYGALDDEAFVTLLYNNVLDRDPD 981          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: A0A2R4GYV0_RHOPL (DUF4214 domain-containing protein n=1 Tax=Rhodopseudomonas palustris TaxID=1076 RepID=A0A2R4GYV0_RHOPL)

HSP 1 Score: 58.9 bits (141), Expect = 5.840e-7
Identity = 44/142 (30.99%), Postives = 61/142 (42.96%), Query Frame = 1
Query:   94 LGHVDRLMSGDPAAAVIETFWKSEEHRALQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPSVYAAE---------MYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPERYNVL 492
            LG V+RL   D   A+        +  A  VY  Y    +R P    L+ WV  L SG     +T   + SSEF        SVY A+         MYR +L R   PE   +W G L+ G+P A++   F  S E + ++
Sbjct:  467 LGSVERLQFVDGVVAL------DLQGHAGDVYRLYKAAFDRTPDTGGLSYWVHGLDSGLDILSVTQGFVNSSEF-------RSVYGADSSNAHIVDLMYRNVLGRPGEPEGTDYWVGNLDRGLPIAELLQGFAVSSENHGLV 595          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: UPI000411C54B (Ig-like domain-containing protein n=1 Tax=Chitinilyticum litopenaei TaxID=1121276 RepID=UPI000411C54B)

HSP 1 Score: 57.0 bits (136), Expect = 3.060e-6
Identity = 46/155 (29.68%), Postives = 69/155 (44.52%), Query Frame = 1
Query:   25 TASYFVAGLYETLLGRTADTGSLLGHVDRLMSGD-PAAAVIETFWKSEEHR--ALQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPS-VYAAEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPE 477
            ++ +FV  LY  LL R A+   L    D+L  G    A     F  S E +  A  +   Y   LER+P    L  W+TQ   G++   +   L+ ++EF  L   + +  Y   +Y+ +L R A    + +WAGQL+ GV    V      S E
Sbjct: 1672 SSKFFVMQLYRDLLFREAEPAGLAYWQDQLDRGVLTRAETAAIFLDSPEFQQAAGGISRLYLGALERLPDDAGLNAWMTQALKGSTLPQIADQLVATAEFQGLYGGLGNRQYVERLYQSVLERTADAAGLDYWAGQLDQGVSRGTVLLGLSESAE 1826          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: A0A3D9EKM6_PSEOL (V8-like Glu-specific endopeptidase n=1 Tax=Pseudomonas oleovorans TaxID=301 RepID=A0A3D9EKM6_PSEOL)

HSP 1 Score: 52.8 bits (125), Expect = 7.690e-5
Identity = 27/96 (28.12%), Postives = 46/96 (47.92%), Query Frame = 1
Query:  205 VLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPSV-YAAEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPERYNV 489
            V +R+P    L  W+ +LA+G +   +  +   SSEF      + +  +A ++Y  ILHR      + +W GQL+ G+        F NS E + +
Sbjct:  550 VFDRMPETGGLQYWIDKLAAGTTFQTIANTFSQSSEFAQRYNAVDNTGFAQQLYSTILHRNGDQGGIDYWKGQLDQGMSRGTAMVQFTNSTENHQL 645          
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Match: A0A8E6B578_9BACT (DUF4214 domain-containing protein n=1 Tax=Telmatocola sphagniphila TaxID=1123043 RepID=A0A8E6B578_9BACT)

HSP 1 Score: 52.8 bits (125), Expect = 8.190e-5
Identity = 31/108 (28.70%), Postives = 53/108 (49.07%), Query Frame = 1
Query:  157 KSEEHRALQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLLTSSEFVSLLKNIPSVYAAEMYRLILHREAAPEEVQFWAGQLENGVPHADVANAFLNSPER 480
            +S E+ +  +  AY + L R P    LA+W++Q+  G ++  +  + + S E+++      + +   MY  +L R  +  EVQ W  QL  GV    +A  F  S ER
Sbjct:  818 QSAEYYSNFITAAYQKYLGRSPDKSGLANWISQMQHGLTDEQLEANFIGSPEYIANHGGAGAGWIKGMYVDLLGRSPSASEVQGWVNQLNAGVSTTQIAYGFAASQER 925          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81448.19055.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
UPI002025AC952.510e-930.46DUF4214 domain-containing protein n=1 Tax=Rhodovas... [more]
A0A1J5QHG7_9ZZZZ9.600e-840.30Extracellular serine proteinase n=1 Tax=mine drain... [more]
A0A512JB58_9HYPH2.300e-730.22Uncharacterized protein n=1 Tax=Methylobacterium o... [more]
UPI001AAF2E532.440e-724.57DUF4214 domain-containing protein n=3 Tax=unclassi... [more]
Q0FQV3_SALBH4.730e-733.08Hemolysin-type calcium-binding region n=2 Tax=Sali... [more]
A0A2R4GYV0_RHOPL5.840e-730.99DUF4214 domain-containing protein n=1 Tax=Rhodopse... [more]
UPI000411C54B3.060e-629.68Ig-like domain-containing protein n=1 Tax=Chitinil... [more]
A0A3D9EKM6_PSEOL7.690e-528.13V8-like Glu-specific endopeptidase n=1 Tax=Pseudom... [more]
A0A8E6B578_9BACT8.190e-528.70DUF4214 domain-containing protein n=1 Tax=Telmatoc... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81448contigM-pyrifera_M_contig81448:256..792 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score85.9
Seed ortholog evalue2.1e-14
Seed eggNOG ortholog1380354.JIAN01000005_gene1278
Hectar predicted targeting categoryother localisation
EggNOG free text desc.COG2335, Secreted and surface protein containing fasciclin-like repeats
EggNOG OGsCOG2335@1,COG2335@2
COG Functional cat.M
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size537
Cds size537
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816721.0186079-CDS-M-pyrifera_M_contig81448:255..7921622816721.0186079-CDS-M-pyrifera_M_contig81448:255..792Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81448 256..792 -
1692278139.7153068-CDS-M-pyrifera_M_contig81448:255..7921692278139.7153068-CDS-M-pyrifera_M_contig81448:255..792Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81448 256..792 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81448.19055.1prot_M-pyrifera_M_contig81448.19055.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81448 256..792 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81448.19055.1

>prot_M-pyrifera_M_contig81448.19055.1 ID=prot_M-pyrifera_M_contig81448.19055.1|Name=mRNA_M-pyrifera_M_contig81448.19055.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=179bp
QPVAADRVTASYFVAGLYETLLGRTADTGSLLGHVDRLMSGDPAAAVIET
FWKSEEHRALQVYEAYAEVLERIPAADELADWVTQLASGASENDMTLSLL
TSSEFVSLLKNIPSVYAAEMYRLILHREAAPEEVQFWAGQLENGVPHADV
ANAFLNSPERYNVLLDRYYNDFLGRPLDP
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mRNA from alignment at M-pyrifera_M_contig81448:256..792-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81448.19055.1 ID=mRNA_M-pyrifera_M_contig81448.19055.1|Name=mRNA_M-pyrifera_M_contig81448.19055.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=537bp|location=Sequence derived from alignment at M-pyrifera_M_contig81448:256..792- (Macrocystis pyrifera P11B4 male)
CAACCGGTGGCTGCCGACCGCGTGACCGCTTCATACTTTGTGGCGGGCCT CTATGAAACGTTACTCGGACGCACCGCCGATACGGGCAGCCTGCTCGGTC ACGTAGATCGCCTTATGTCAGGTGATCCAGCGGCGGCGGTCATTGAAACC TTTTGGAAGTCCGAGGAGCATCGAGCCCTCCAGGTTTACGAAGCCTACGC TGAGGTGCTGGAACGGATTCCCGCTGCCGACGAACTTGCCGACTGGGTGA CGCAGCTCGCCTCGGGTGCCTCTGAAAATGACATGACGTTGAGCCTGCTG ACTTCCAGCGAGTTCGTCTCCTTGCTCAAGAACATTCCGTCGGTCTACGC AGCCGAGATGTACCGACTGATACTCCACCGCGAAGCCGCCCCGGAAGAAG TTCAATTCTGGGCGGGGCAGCTTGAGAATGGTGTTCCGCACGCGGATGTG GCGAACGCCTTCTTGAACAGCCCCGAGCGATACAATGTTCTTCTGGATCG CTACTACAACGACTTCCTCGGACGTCCGTTGGATCCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81448:256..792-

>mRNA_M-pyrifera_M_contig81448.19055.1 ID=mRNA_M-pyrifera_M_contig81448.19055.1|Name=mRNA_M-pyrifera_M_contig81448.19055.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1074bp|location=Sequence derived from alignment at M-pyrifera_M_contig81448:256..792- (Macrocystis pyrifera P11B4 male)
CAACCGGTGGCTGCCGACCGCGTGACCGCTTCATACTTTGTGGCGGGCCT
CTATGAAACGTTACTCGGACGCACCGCCGATACGGGCAGCCTGCTCGGTC
ACGTAGATCGCCTTATGTCAGGTGATCCAGCGGCGGCGGTCATTGAAACC
TTTTGGAAGTCCGAGGAGCATCGAGCCCTCCAGGTTTACGAAGCCTACGC
TGAGGTGCTGGAACGGATTCCCGCTGCCGACGAACTTGCCGACTGGGTGA
CGCAGCTCGCCTCGGGTGCCTCTGAAAATGACATGACGTTGAGCCTGCTG
ACTTCCAGCGAGTTCGTCTCCTTGCTCAAGAACATTCCGTCGGTCTACGC
AGCCGAGATGTACCGACTGATACTCCACCGCGAAGCCGCCCCGGAAGAAG
TTCAATTCTGGGCGGGGCAGCTTGAGAATGGTGTTCCGCACGCGGATGTG
GCGAACGCCTTCTTGAACAGCCCCGAGCGATACAATGTTCTTCTGGATCG
CTACTACAACGACTTCCTCGGACGTCCGTTGGATCCTCAACCGGTGGCTG
CCGACCGCGTGACCGCTTCATACTTTGTGGCGGGCCTCTATGAAACGTTA
CTCGGACGCACCGCCGATACGGGCAGCCTGCTCGGTCACGTAGATCGCCT
TATGTCAGGTGATCCAGCGGCGGCGGTCATTGAAACCTTTTGGAAGTCCG
AGGAGCATCGAGCCCTCCAGGTTTACGAAGCCTACGCTGAGGTGCTGGAA
CGGATTCCCGCTGCCGACGAACTTGCCGACTGGGTGACGCAGCTCGCCTC
GGGTGCCTCTGAAAATGACATGACGTTGAGCCTGCTGACTTCCAGCGAGT
TCGTCTCCTTGCTCAAGAACATTCCGTCGGTCTACGCAGCCGAGATGTAC
CGACTGATACTCCACCGCGAAGCCGCCCCGGAAGAAGTTCAATTCTGGGC
GGGGCAGCTTGAGAATGGTGTTCCGCACGCGGATGTGGCGAACGCCTTCT
TGAACAGCCCCGAGCGATACAATGTTCTTCTGGATCGCTACTACAACGAC
TTCCTCGGACGTCCGTTGGATCCT
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