mRNA_M-pyrifera_M_contig81319.19023.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81319.19023.1
Unique NamemRNA_M-pyrifera_M_contig81319.19023.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A0M0JJ24_9EUKA (Alpha,alpha-trehalose-phosphate synthase n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0JJ24_9EUKA)

HSP 1 Score: 176 bits (447), Expect = 1.020e-46
Identity = 104/257 (40.47%), Postives = 143/257 (55.64%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSALRSHFLPRLPPKKRDTPTQSPLLSPTPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSQLDGRVVIVGVDSWERLDGLSLKLLALDRLLRRVPELVGRV 786
            W AY  AN+RF DKV+E Y  GDLVWVH +HLLLLP +I R+L   A VGLFLH+PFPSSE+FRT+ VR +++R ML AD +GFH++EYARHF+T C+R+LGL  E    G + V    R + I V H G+EP L + + A     ++ +AL     P +P       + S  +S + P                                     Q  G+ V++G+D  ERL G+ LKL+A ++LL+ VPE   +V
Sbjct:  261 WRAYCAANRRFADKVIEVYDSGDLVWVHDYHLLLLPSYILRKLRT-ARVGLFLHTPFPSSEIFRTIPVREELIRGMLNADLIGFHIFEYARHFLTCCKRMLGLEYEFHPGGFIAVRDYKRDVKISVSHVGIEPELCRVEQA---AGEANTALAGGAAPPMPWACAGFVSVSSGVSLSSPVVANEEARKLASNLVELRQL-----------------QAAGKTVMLGIDEMERLKGIPLKLIAFEQLLKTVPERADKV 496          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A7S0J819_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0J819_9EUKA)

HSP 1 Score: 164 bits (416), Expect = 2.440e-46
Identity = 75/146 (51.37%), Postives = 104/146 (71.23%), Query Frame = 1
Query:    7 AKRWAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALP 444
            A RW AY   N+RF +KV+E Y  GD++W+H +HLLLLP  + R+L   A VGLFLH+PFPSSE+FRT+SVR+++LR ML AD +GFH++EYARHF+T C+R+LGL+      G + VL    ++ + V HAG++P L+    A P
Sbjct:  100 ASRWRAYCTVNRRFAEKVIEVYEPGDVIWIHDYHLLLLPSVVLRKLRT-ARVGLFLHTPFPSSEIFRTISVRDELLRGMLNADIVGFHVFEYARHFLTCCKRMLGLSCHFLPGGAIGVLEDNHSVLVQVTHAGIDPELLTEALAAP 244          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A7S0K1R9_CAFRO (Hypothetical protein (Fragment) n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0K1R9_CAFRO)

HSP 1 Score: 172 bits (436), Expect = 3.550e-46
Identity = 91/163 (55.83%), Postives = 111/163 (68.10%), Query Frame = 1
Query:    4 QAKRWAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAP-VGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLR-------VLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSA 468
            Q  RW AYS  NQ FTDKVV+ Y +GDLVW HGHHLLL+P FIARR+    P VGLFLH+PFPSSE+FR+LS R D+LR ML +  LGFHL+EYARHF T+CRRIL L+   D   R R       +  QGR +++ V HAG+EP++        + RD RSA
Sbjct:  357 QEDRWQAYSVVNQAFTDKVVQRYSEGDLVWAHGHHLLLMPQFIARRIRKATPSVGLFLHAPFPSSEIFRSLSCRQDLLRGMLLSGQLGFHLFEYARHFSTSCRRILSLHESDDAGERDRGHPGSQIIEYQGRNVSLTVSHAGIEPSV--------FARDVRSA 511          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A7S2RRW3_9STRA (Hypothetical protein n=2 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2RRW3_9STRA)

HSP 1 Score: 174 bits (440), Expect = 1.060e-45
Identity = 86/144 (59.72%), Postives = 107/144 (74.31%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAP-VGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLN---SEVDEEGRLRVLA-QGRTIAIYVCHAGLEPNLVKHK 432
            W AY   NQ F +K+VE Y++GD++W+HG  LLLLP  +ARRL    P +GLF+HSPFPSSEVFRTLSVR+DILR ML AD +GFHLYEYAR F++ C RILG+    +E D +G +  L  QGR + I VCHAG+EP+LVK K
Sbjct:  349 WKAYRSVNQLFAEKIVEVYNEGDIIWIHGLELLLLPSLLARRLQAGNPHIGLFIHSPFPSSEVFRTLSVRDDILRGMLNADQIGFHLYEYARPFISCCHRILGIQHHGNENDSKGGVLTLEYQGRRVCITVCHAGIEPDLVKAK 492          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: V5K3Y9_SACJA (Trehalose-6-phosohate synthase n=1 Tax=Saccharina japonica TaxID=88149 RepID=V5K3Y9_SACJA)

HSP 1 Score: 172 bits (437), Expect = 2.640e-45
Identity = 82/157 (52.23%), Postives = 108/157 (68.79%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNS-EVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSALRSHF 483
            W AY+  N+RF DK+VE Y++GD+VWVHG HLLLLP F+ R L   A VGLFLH+PFPSSE+FRTL  R D+LR ML AD +GFHL+EYARHF+T C+RILGLN  + +  G   V   GR ++I   H G+EP+++    + P +    + LR  +
Sbjct:  294 WQAYANVNRRFRDKIVEVYNEGDMVWVHGFHLLLLPSFLTRVLRT-ARVGLFLHTPFPSSEIFRTLPFREDLLRGMLNADQIGFHLFEYARHFLTCCKRILGLNEGDSNNRGNTNVFYNGRCVSIESMHGGIEPSIIAANLSNPGIVGRAAELRKEY 449          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A5A8EN89_CAFRO (CBM20 domain-containing protein n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8EN89_CAFRO)

HSP 1 Score: 172 bits (436), Expect = 3.810e-45
Identity = 91/163 (55.83%), Postives = 111/163 (68.10%), Query Frame = 1
Query:    4 QAKRWAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAP-VGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLR-------VLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSA 468
            Q  RW AYS  NQ FTDKVV+ Y +GDLVW HGHHLLL+P FIARR+    P VGLFLH+PFPSSE+FR+LS R D+LR ML +  LGFHL+EYARHF T+CRRIL L+   D   R R       +  QGR +++ V HAG+EP++        + RD RSA
Sbjct:  329 QEDRWQAYSVVNQAFTDKVVQRYSEGDLVWAHGHHLLLMPQFIARRIRKATPSVGLFLHAPFPSSEIFRSLSCRQDLLRGMLLSGQLGFHLFEYARHFSTSCRRILSLHESDDAGERDRGHPGSQIIEYQGRNVSLTVSHAGIEPSV--------FARDVRSA 483          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A7S0E497_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0E497_9EUKA)

HSP 1 Score: 162 bits (409), Expect = 5.370e-45
Identity = 76/144 (52.78%), Postives = 104/144 (72.22%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLRVLAQGRTIAIYVCHAGLEPNLV--KHKTAL 441
            W AY   N+R  DKV+E Y+ GD+VWVH +HLLLLP +I R+L     VGLFLH PFPSSE+FRT+SVR+++LR ML AD LGFH++EYARHF+T C+R+LGL+ E  + G + V    R + + V H G+EP+++   H++ L
Sbjct:   79 WRAYCTVNRRLADKVIEIYNSGDVVWVHDYHLLLLPSYILRKLRT-TRVGLFLHIPFPSSEIFRTISVRDELLRGMLNADLLGFHIFEYARHFLTCCKRLLGLDYEFQQGGYIGVRDHKRNVMVQVSHMGIEPSVLLEAHRSGL 221          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: D7FL73_ECTSI (Trehalose 6-phosphate synthase, family GT20 / Trehalose 6-phosphate phosphatase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FL73_ECTSI)

HSP 1 Score: 169 bits (429), Expect = 3.080e-44
Identity = 81/157 (51.59%), Postives = 106/157 (67.52%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNS-EVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSALRSHF 483
            W  Y+  N+RF DK+VE Y++GD++WVHG HLLLLP F+ R L   A VGLFLH+PFPSSE+FRTL  R D+LR ML AD +GFHL+EYARHF+T C+RILGLN  +    G   V   GR ++I   H G+EP+++  K     +    +ALR  +
Sbjct:  254 WQCYANVNRRFRDKIVEVYNEGDMIWVHGFHLLLLPSFLTRVLRT-ARVGLFLHTPFPSSEIFRTLPFREDLLRGMLNADQIGFHLFEYARHFITCCKRILGLNEGDSTNRGNRNVYYNGRCVSIESIHGGIEPSIIAAKLGHTDIAGRVAALRKEY 409          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A485KSP4_9STRA (Aste57867_11350 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KSP4_9STRA)

HSP 1 Score: 169 bits (429), Expect = 3.080e-44
Identity = 83/156 (53.21%), Postives = 108/156 (69.23%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSALRSHF 483
            W++Y   N+ F +KV+E Y++GDLVWVHG HLL+ P F+ARRL +   VGLFLH+PFPSSE+FRTLSVR D+LR ML +D +GFHLYE+ARHF+T+CRRILGL       G + V   GR +AI + H G+EP  V   +    V    + LRS +
Sbjct:  450 WSSYMHVNRMFVNKVIEVYNEGDLVWVHGLHLLVAPSFLARRLPY-VNVGLFLHTPFPSSEIFRTLSVRADLLRGMLSSDHIGFHLYEHARHFLTSCRRILGLKYNAQAGGYIGVEYNGRMVAITISHIGIEPPFVDRLSMKDQVVQETARLRSLY 604          
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Match: A0A6A5AEY3_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A5AEY3_9STRA)

HSP 1 Score: 168 bits (425), Expect = 3.460e-44
Identity = 82/156 (52.56%), Postives = 108/156 (69.23%), Query Frame = 1
Query:   16 WAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHHHAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVTACRRILGLNSEVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALPWVRDSRSALRSHF 483
            W++Y   N+ F +KV+E Y++GDLVWVHG HLL+ P F++RRL +   VGLFLH+PFPSSE+FRTLSVR D+LR ML AD +GFHLYE+ARHF+T+CRRILGL       G + V   GR +AI + H G+EP  V   +    V    + LR+ +
Sbjct:   81 WSSYMHVNRIFVNKVIEVYNEGDLVWVHGLHLLVAPSFLSRRLPY-VNVGLFLHTPFPSSEIFRTLSVRADLLRGMLSADHIGFHLYEHARHFLTSCRRILGLKYNAQAGGYIGVEYNGRMVAITISHIGIEPPFVDRLSVKDQVLHETARLRALY 235          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81319.19023.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0M0JJ24_9EUKA1.020e-4640.47Alpha,alpha-trehalose-phosphate synthase n=1 Tax=C... [more]
A0A7S0J819_9EUKA2.440e-4651.37Hypothetical protein (Fragment) n=1 Tax=Calcidiscu... [more]
A0A7S0K1R9_CAFRO3.550e-4655.83Hypothetical protein (Fragment) n=1 Tax=Cafeteria ... [more]
A0A7S2RRW3_9STRA1.060e-4559.72Hypothetical protein n=2 Tax=labyrinthulid quahog ... [more]
V5K3Y9_SACJA2.640e-4552.23Trehalose-6-phosohate synthase n=1 Tax=Saccharina ... [more]
A0A5A8EN89_CAFRO3.810e-4555.83CBM20 domain-containing protein n=4 Tax=Cafeteria ... [more]
A0A7S0E497_9EUKA5.370e-4552.78Hypothetical protein (Fragment) n=1 Tax=Phaeocysti... [more]
D7FL73_ECTSI3.080e-4451.59Trehalose 6-phosphate synthase, family GT20 / Treh... [more]
A0A485KSP4_9STRA3.080e-4453.21Aste57867_11350 protein n=1 Tax=Aphanomyces stella... [more]
A0A6A5AEY3_9STRA3.460e-4452.56Uncharacterized protein (Fragment) n=1 Tax=Aphanom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81319contigM-pyrifera_M_contig81319:3..932 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score174.1
Seed ortholog evalue8.6e-41
Seed eggNOG ortholog112098.XP_008609543.1
KEGG rclassRC00005,RC00017,RC00049,RC02748
KEGG koko:K16055
KEGG ReactionR02737,R02778
KEGG Pathwayko00500,ko01100,map00500,map01100
Hectar predicted targeting categoryother localisation
EggNOG free text desc.trehalose biosynthetic process
EggNOG OGsCOG1877@1,KOG1050@2759
EC2.4.1.15,3.1.3.12
COG Functional cat.G
CAZyGT20
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01003
Exons2
Model size786
Cds size786
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816719.5877793-CDS-M-pyrifera_M_contig81319:2..6701622816719.5877793-CDS-M-pyrifera_M_contig81319:2..670Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81319 3..670 +
1692278139.0198345-CDS-M-pyrifera_M_contig81319:2..6701692278139.0198345-CDS-M-pyrifera_M_contig81319:2..670Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81319 3..670 +
1622816719.5983944-CDS-M-pyrifera_M_contig81319:814..9321622816719.5983944-CDS-M-pyrifera_M_contig81319:814..932Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81319 815..932 +
1692278139.0303795-CDS-M-pyrifera_M_contig81319:814..9321692278139.0303795-CDS-M-pyrifera_M_contig81319:814..932Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81319 815..932 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81319.19023.1prot_M-pyrifera_M_contig81319.19023.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81319 3..932 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81319.19023.1

>prot_M-pyrifera_M_contig81319.19023.1 ID=prot_M-pyrifera_M_contig81319.19023.1|Name=mRNA_M-pyrifera_M_contig81319.19023.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=262bp
RQAKRWAAYSEANQRFTDKVVEAYHDGDLVWVHGHHLLLLPGFIARRLHH
HAPVGLFLHSPFPSSEVFRTLSVRNDILRAMLQADSLGFHLYEYARHFVT
ACRRILGLNSEVDEEGRLRVLAQGRTIAIYVCHAGLEPNLVKHKTALPWV
RDSRSALRSHFLPRLPPKKRDTPTQSPLLSPTPPAPQPLPSPSTTTSTTT
TPTSSSSSSSSSSSSSSSSSSQLDGRVVIVGVDSWERLDGLSLKLLALDR
LLRRVPELVGRV
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mRNA from alignment at M-pyrifera_M_contig81319:3..932+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81319.19023.1 ID=mRNA_M-pyrifera_M_contig81319.19023.1|Name=mRNA_M-pyrifera_M_contig81319.19023.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=930bp|location=Sequence derived from alignment at M-pyrifera_M_contig81319:3..932+ (Macrocystis pyrifera P11B4 male)
AGGCAGGCCAAGCGCTGGGCTGCTTACTCCGAGGCGAACCAGCGGTTCAC AGACAAGGTCGTGGAAGCCTATCACGACGGCGACCTCGTGTGGGTGCATG GCCATCACCTGCTGCTGCTCCCGGGATTCATCGCAAGGCGGCTGCACCAC CACGCGCCAGTGGGCCTCTTCCTCCACTCCCCGTTCCCGTCCTCTGAGGT GTTCCGCACTCTGTCAGTCAGAAACGACATTCTCCGCGCCATGCTACAAG CAGATTCACTCGGCTTCCACCTCTACGAATACGCCAGACACTTTGTCACG GCCTGCCGCCGCATCCTTGGCCTCAACTCGGAAGTGGACGAGGAAGGCAG GCTGCGAGTGCTGGCACAGGGCAGAACCATCGCCATCTACGTCTGCCACG CAGGCCTCGAACCCAACCTGGTCAAACACAAGACGGCACTCCCGTGGGTC AGAGACTCGAGGTCTGCGCTCCGCTCCCACTTCCTCCCAAGACTGCCTCC CAAGAAGAGGGATACTCCCACCCAATCTCCTCTCCTCTCCCCAACCCCGC CTGCACCACAACCTCTCCCCTCCCCCTCCACCACAACCTCCACCACCACC ACCCCAACCTCCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTC TTCTTCTTCCTCGCAATTGTCGCGGTGGAGAAGGAGTGGCTGGGGCGTGG TGGAGGCGGAGGAGGAGGGGCGGCTGCAGGTGCCGCTGTACCAGCGGTTT ATGGACAAGGAGAGGGAGTGGCTGGGTCTGTTTGGGGGTGGAGGTGGAGG TGTGGGTGGCAGGGATGGGCGGGTGGTGATTGTGGGCGTGGATTCGTGGG AGCGGCTGGATGGGCTGAGTCTCAAGCTCTTGGCGCTGGACCGTTTGTTG CGACGAGTGCCAGAGTTGGTGGGTCGGGTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81319:3..932+

>mRNA_M-pyrifera_M_contig81319.19023.1 ID=mRNA_M-pyrifera_M_contig81319.19023.1|Name=mRNA_M-pyrifera_M_contig81319.19023.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1572bp|location=Sequence derived from alignment at M-pyrifera_M_contig81319:3..932+ (Macrocystis pyrifera P11B4 male)
AGGCAGGCCAAGCGCTGGGCTGCTTACTCCGAGGCGAACCAGCGGTTCAC
AGACAAGGTCGTGGAAGCCTATCACGACGGCGACCTCGTGTGGGTGCATG
GCCATCACCTGCTGCTGCTCCCGGGATTCATCGCAAGGCGGCTGCACCAC
CACGCGCCAGTGGGCCTCTTCCTCCACTCCCCGTTCCCGTCCTCTGAGGT
GTTCCGCACTCTGTCAGTCAGAAACGACATTCTCCGCGCCATGCTACAAG
CAGATTCACTCGGCTTCCACCTCTACGAATACGCCAGACACTTTGTCACG
GCCTGCCGCCGCATCCTTGGCCTCAACTCGGAAGTGGACGAGGAAGGCAG
GCTGCGAGTGCTGGCACAGGGCAGAACCATCGCCATCTACGTCTGCCACG
CAGGCCTCGAACCCAACCTGGTCAAACACAAGACGGCACTCCCGTGGGTC
AGAGACTCGAGGTCTGCGCTCCGCTCCCACTTCCTCCCAAGACTGCCTCC
CAAGAAGAGGGATACTCCCACCCAATCTCCTCTCCTCTCCCCAACCCCGC
CTGCACCACAACCTCTCCCCTCCCCCTCCACCACAACCTCCACCACCACC
ACCCCAACCTCCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTC
TTCTTCTTCCTCGCAATTAGGCAGGCCAAGCGCTGGGCTGCTTACTCCGA
GGCGAACCAGCGGTTCACAGACAAGGTCGTGGAAGCCTATCACGACGGCG
ACCTCGTGTGGGTGCATGGCCATCACCTGCTGCTGCTCCCGGGATTCATC
GCAAGGCGGCTGCACCACCACGCGCCAGTGGGCCTCTTCCTCCACTCCCC
GTTCCCGTCCTCTGAGGTGTTCCGCACTCTGTCAGTCAGAAACGACATTC
TCCGCGCCATGCTACAAGCAGATTCACTCGGCTTCCACCTCTACGAATAC
GCCAGACACTTTGTCACGGCCTGCCGCCGCATCCTTGGCCTCAACTCGGA
AGTGGACGAGGAAGGCAGGCTGCGAGTGCTGGCACAGGGCAGAACCATCG
CCATCTACGTCTGCCACGCAGGCCTCGAACCCAACCTGGTCAAACACAAG
ACGGCACTCCCGTGGGTCAGAGACTCGAGGTCTGCGCTCCGCTCCCACTT
CCTCCCAAGACTGCCTCCCAAGAAGAGGGATACTCCCACCCAATCTCCTC
TCCTCTCCCCAACCCCGCCTGCACCACAACCTCTCCCCTCCCCCTCCACC
ACAACCTCCACCACCACCACCCCAACCTCCTCCTCTTCCTCCTCCTCCTC
CTCCTCCTCCTCCTCTTCTTCTTCTTCCTCGCAATTGGATGGGCGGGTGG
TGATTGTGGGCGTGGATTCGTGGGAGCGGCTGGATGGGCTGAGTCTCAAG
CTCTTGGCGCTGGACCGTTTGTTGCGACGAGTGCCAGAGTTGGTGGGTCG
GGTGGGATGGGCGGGTGGTGATTGTGGGCGTGGATTCGTGGGAGCGGCTG
GATGGGCTGAGTCTCAAGCTCTTGGCGCTGGACCGTTTGTTGCGACGAGT
GCCAGAGTTGGTGGGTCGGGTG
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