mRNA_M-pyrifera_M_contig81130.18977.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81130.18977.1
Unique NamemRNA_M-pyrifera_M_contig81130.18977.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: F2U6W4_SALR5 (Sialidase domain-containing protein n=1 Tax=Salpingoeca rosetta (strain ATCC 50818 / BSB-021) TaxID=946362 RepID=F2U6W4_SALR5)

HSP 1 Score: 160 bits (406), Expect = 8.770e-44
Identity = 87/198 (43.94%), Postives = 120/198 (60.61%), Query Frame = 1
Query:    1 YALYDTDEQHGPDQWRCYSASSLDPTHSHYVNGSSYCTREQQLTQLMGNCAKGVETTINPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQDASQPSP---TMMHNGMLNLQLDHQGNSTQMLGTIKGTSISFSPPTSLAKYLGPANGAAVGPGV 585
            YA +DT+ QHGP QWRCYS+ +LD  +  YV G+ YCTR++Q+  ++ +C   V  T+  VF  G+ GYPCIRIPS++    G L AFAECR+W GDGCEP G    E    D CMK S D G TW  L V+L++A+QP+    T+ +  +LN       ++ Q++    G +  +S P  L  +LG A  + VGPGV
Sbjct:   78 YARFDTNAQHGPKQWRCYSSDALDSRNQSYVKGTGYCTRDEQIRAVIADCEANV--TLVDVFTPGEAGYPCIRIPSLVLVGDGSLLAFAECRNWTGDGCEPTGVH-AENSNRDICMKRSRDSGHTWSPLKVILRNAAQPTAVFDTVHNQTVLNYIQLQPASNLQVVSFDNGNT--WSTPLHLDAFLGGAAPSDVGPGV 270          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A9V8C6_MONBE (Predicted protein n=1 Tax=Monosiga brevicollis TaxID=81824 RepID=A9V8C6_MONBE)

HSP 1 Score: 125 bits (315), Expect = 9.110e-31
Identity = 71/147 (48.30%), Postives = 85/147 (57.82%), Query Frame = 1
Query:    1 YALYDTDEQHGPDQWRCYSASSLDPTHSHYVNGSSYCTREQQLTQLMGNCAKGVETTINPVFMHGDGGYPCIRIPSILQAPSG-VLFAFAECRHWIGDGCEPAGQKGVEGGTT-----DACMKSSGDGGKTWGNLTVVLQDASQPSP 423
            YA YD        QWRCYS SSL P  S Y NG+SYCTREQ++  ++ +C   V  T   VF  G+ GYPCIRIPSIL A     L AFAECR+W GDGCEP G +     +T     D CMK       TW  L V+ ++A+QP P
Sbjct:   63 YARYDVAANDDEKQWRCYSPSSLTPDLSGYSNGTSYCTREQEILDVIADC--DVNRTQIAVFTPGELGYPCIRIPSILLAGDNRTLLAFAECRNWTGDGCEPQGFEDARYNSTSNANRDLCMK-------TWSALHVIQRNAAQPMP 200          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A0A649UZH4_9STRA (Sialidase n=1 Tax=Opalinidae sp. TaxID=2059444 RepID=A0A649UZH4_9STRA)

HSP 1 Score: 90.5 bits (223), Expect = 4.470e-18
Identity = 44/76 (57.89%), Postives = 53/76 (69.74%), Query Frame = 1
Query:  178 PVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQKGV---EGGTTDA--CMKSSGDGGKTWGNLT 390
            PVF+HG+GGYPCIRIP+I++  +G L AFAECR +IGDGC P   K     EG T D   C+KSS D G TW NL+
Sbjct:   26 PVFVHGEGGYPCIRIPAIIRTHTGKLLAFAECRKFIGDGCTPTDGKEYPKQEGATLDGRICLKSSTDDGYTWSNLS 101          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A0A649UZY4_9STRA (Sialidase (Fragment) n=1 Tax=Opalinidae sp. TaxID=2059444 RepID=A0A649UZY4_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 9.180e-18
Identity = 41/78 (52.56%), Postives = 53/78 (67.95%), Query Frame = 1
Query:  178 PVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAG-----QKGVEGGTTDA--CMKSSGDGGKTWGNLT 390
            PVF+HG+GGYPCIRIP+I++  +G L AFAECR + GDGC P       ++  EG T D   C+K+S D G TW NL+
Sbjct:   26 PVFIHGEGGYPCIRIPAIIRTDTGKLLAFAECRRFTGDGCNPTNGDEYPKQEQEGATYDGRICLKTSVDDGYTWSNLS 103          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A0A649UYU6_9STRA (Sialidase (Fragment) n=1 Tax=Opalina sp. OP10 TaxID=2666322 RepID=A0A649UYU6_9STRA)

HSP 1 Score: 76.3 bits (186), Expect = 3.960e-13
Identity = 39/83 (46.99%), Postives = 52/83 (62.65%), Query Frame = 1
Query:  160 VETTINPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQ-KGVEGGTTDA--CMKSSGDGGKTWGNLTVVL 399
            V TT+  +F HG+G +PCIRIP++++  +G L AFAECR + GDGC P    +G      DA  C K S D GKTW +L+  L
Sbjct:    3 VYTTV--IFQHGEGKWPCIRIPAVVKCGTGRLLAFAECRRFTGDGCNPTNYIRGDNENAYDAVICQKYSDDNGKTWSDLSYPL 83          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: UPI00191E3659 (glycoside hydrolase n=1 Tax=Streptomyces sp. 7-21 TaxID=2802283 RepID=UPI00191E3659)

HSP 1 Score: 66.2 bits (160), Expect = 2.290e-9
Identity = 38/83 (45.78%), Postives = 47/83 (56.63%), Query Frame = 1
Query:  160 VETTINPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECR-HWIGDGCEPAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQD 405
            ++T    VF  G+GGY   R+P+++QAP G L AFAE R +  GD            G  D  MK S DGG+TWG LTVVL D
Sbjct:   42 LQTAYATVFTKGEGGYHTFRVPAVVQAPDGSLLAFAEGRVNGPGDD-----------GDIDLVMKRSRDGGRTWGPLTVVLDD 113          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: UPI001F587CAB (glycoside hydrolase n=2 Tax=unclassified Aestuariivivens TaxID=2626726 RepID=UPI001F587CAB)

HSP 1 Score: 63.2 bits (152), Expect = 2.500e-8
Identity = 42/143 (29.37%), Postives = 65/143 (45.45%), Query Frame = 1
Query:  112 TREQQLTQLMGNCAKGVETTINPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQDASQ----PSPTM-MHNGML------NLQLDHQGNSTQMLGTIKGT 507
            +++++  Q  G   +    T+NP+F+ G  GY C RIP+I+    G + AF+E R    + C   G         D  M+ S D G +WG + V+  D       P+P +    G +      NL  DH+    +  GT KGT
Sbjct:   22 SKDKRHPQAPGTTLQNENITLNPIFIKGQNGYACFRIPAIVVTHDGSILAFSEARK---NSCSDTGD-------IDLVMRKSTDNGTSWGEIKVIWDDGENVCGNPAPVIDAETGRIHLLSTWNLGEDHESEIIE--GTSKGT 152          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A0A1Q4F366_9SPHI (Exo-alpha-sialidase n=1 Tax=Sphingobacteriales bacterium 44-15 TaxID=1895837 RepID=A0A1Q4F366_9SPHI)

HSP 1 Score: 62.8 bits (151), Expect = 3.320e-8
Identity = 40/110 (36.36%), Postives = 52/110 (47.27%), Query Frame = 1
Query:  172 INPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQDASQ----PSPTMMHN-------GMLNLQLDHQ 468
            +N +F  G+  Y C RIP+I+   +G L AFAE R    +GC   G         D  +K S DGGKTWG + VV  DA      P+P +  N          NL  DH+
Sbjct:   28 LNHLFKPGESDYKCFRIPAIVVTNNGTLLAFAEARK---NGCSDTGD-------IDLVLKRSADGGKTWGAVEVVWNDAENTCGNPAPVVDRNTGKIILLSTWNLGTDHE 127          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A0A840TTV4_9BACT (Sialidase-1 n=2 Tax=Rhabdobacter roseus TaxID=1655419 RepID=A0A840TTV4_9BACT)

HSP 1 Score: 61.2 bits (147), Expect = 1.180e-7
Identity = 35/85 (41.18%), Postives = 45/85 (52.94%), Query Frame = 1
Query:  181 VFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQ----DASQPSP 423
            VF +G  GY C RIP+I++AP G L AFAE R      C   G         D  +++S DGGKTWG  T+V+      A  P+P
Sbjct:   41 VFQNGSDGYQCYRIPAIVKAPEGTLLAFAEGRR---TNCGDFGD-------VDIVLRTSRDGGKTWGTQTLVVDYGTNQAGNPAP 115          
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Match: A0A1F3MB76_9BACT (Sialidase domain-containing protein n=2 Tax=Bacteroidetes TaxID=976 RepID=A0A1F3MB76_9BACT)

HSP 1 Score: 59.7 bits (143), Expect = 3.650e-7
Identity = 34/78 (43.59%), Postives = 40/78 (51.28%), Query Frame = 1
Query:  172 INPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCEPAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQD 405
            I  VF++G  GYP  RIP++LQ   G L AF E R  I D               D  +KSS DGG TWG L VV +D
Sbjct:    8 IRDVFVNGRDGYPQYRIPALLQTKQGTLLAFCEGRASINDHA-----------ANDIVLKSSTDGGSTWGPLQVVAED 74          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81130.18977.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 22
Match NameE-valueIdentityDescription
F2U6W4_SALR58.770e-4443.94Sialidase domain-containing protein n=1 Tax=Salpin... [more]
A9V8C6_MONBE9.110e-3148.30Predicted protein n=1 Tax=Monosiga brevicollis Tax... [more]
A0A649UZH4_9STRA4.470e-1857.89Sialidase n=1 Tax=Opalinidae sp. TaxID=2059444 Rep... [more]
A0A649UZY4_9STRA9.180e-1852.56Sialidase (Fragment) n=1 Tax=Opalinidae sp. TaxID=... [more]
A0A649UYU6_9STRA3.960e-1346.99Sialidase (Fragment) n=1 Tax=Opalina sp. OP10 TaxI... [more]
UPI00191E36592.290e-945.78glycoside hydrolase n=1 Tax=Streptomyces sp. 7-21 ... [more]
UPI001F587CAB2.500e-829.37glycoside hydrolase n=2 Tax=unclassified Aestuarii... [more]
A0A1Q4F366_9SPHI3.320e-836.36Exo-alpha-sialidase n=1 Tax=Sphingobacteriales bac... [more]
A0A840TTV4_9BACT1.180e-741.18Sialidase-1 n=2 Tax=Rhabdobacter roseus TaxID=1655... [more]
A0A1F3MB76_9BACT3.650e-743.59Sialidase domain-containing protein n=2 Tax=Bacter... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81130contigM-pyrifera_M_contig81130:352..936 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score164.1
Seed ortholog evalue6.6e-38
Seed eggNOG ortholog946362.XP_004995100.1
Preferred nameNEU1
KEGG rclassRC00028,RC00077
KEGG koko:K01186
KEGG ReactionR04018
KEGG Pathwayko00511,ko00600,ko04142,map00511,map00600,map04142
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0001573,GO:0001775,GO:0002252,GO:0002263,GO:0002274,GO:0002275,GO:0002283,GO:0002366,GO:0002376,GO:0002443,GO:0002444,GO:0002446,GO:0003674,GO:0003824,GO:0004308,GO:0004553,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005773,GO:0005775,GO:0005975,GO:0006629,GO:0006643,GO:0006664,GO:0006665,GO:0006672,GO:0006687,GO:0006689,GO:0006807,GO:0006810,GO:0006887,GO:0006955,GO:0007275,GO:0007399,GO:0008150,GO:0008152,GO:0009056,GO:0009311,GO:0009313,GO:0009986,GO:0009987,GO:0010720,GO:0010975,GO:0010976,GO:0012505,GO:0016020,GO:0016042,GO:0016052,GO:0016192,GO:0016787,GO:0016798,GO:0016997,GO:0019377,GO:0022008,GO:0030054,GO:0030141,GO:0030149,GO:0030154,GO:0031344,GO:0031346,GO:0031410,GO:0031974,GO:0031982,GO:0031983,GO:0032501,GO:0032502,GO:0032940,GO:0034641,GO:0034774,GO:0035580,GO:0036230,GO:0042119,GO:0042127,GO:0042581,GO:0043202,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043299,GO:0043312,GO:0043603,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044255,GO:0044422,GO:0044424,GO:0044433,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0045055,GO:0045321,GO:0045595,GO:0045597,GO:0045664,GO:0045666,GO:0046466,GO:0046479,GO:0046514,GO:0046903,GO:0048518,GO:0048522,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050769,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051094,GO:0051128,GO:0051130,GO:0051179,GO:0051234,GO:0051239,GO:0051240,GO:0051960,GO:0051962,GO:0060205,GO:0060284,GO:0065007,GO:0070013,GO:0071704,GO:0097708,GO:0099503,GO:0120035,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1903509,GO:2000026,GO:2000291
EggNOG free text desc.exo-alpha-(2->3)-sialidase activity
EggNOG OGs2QSIT@2759,39G5C@33154,COG4409@1
EC3.2.1.18
COG Functional cat.G
CAZyGH33
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02042
Exons1
Model size585
Cds size585
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816717.0704694-CDS-M-pyrifera_M_contig81130:351..9361622816717.0704694-CDS-M-pyrifera_M_contig81130:351..936Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81130 352..936 +
1692278137.4133115-CDS-M-pyrifera_M_contig81130:351..9361692278137.4133115-CDS-M-pyrifera_M_contig81130:351..936Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81130 352..936 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81130.18977.1prot_M-pyrifera_M_contig81130.18977.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81130 352..936 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81130.18977.1

>prot_M-pyrifera_M_contig81130.18977.1 ID=prot_M-pyrifera_M_contig81130.18977.1|Name=mRNA_M-pyrifera_M_contig81130.18977.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=195bp
YALYDTDEQHGPDQWRCYSASSLDPTHSHYVNGSSYCTREQQLTQLMGNC
AKGVETTINPVFMHGDGGYPCIRIPSILQAPSGVLFAFAECRHWIGDGCE
PAGQKGVEGGTTDACMKSSGDGGKTWGNLTVVLQDASQPSPTMMHNGMLN
LQLDHQGNSTQMLGTIKGTSISFSPPTSLAKYLGPANGAAVGPGV
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mRNA from alignment at M-pyrifera_M_contig81130:352..936+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81130.18977.1 ID=mRNA_M-pyrifera_M_contig81130.18977.1|Name=mRNA_M-pyrifera_M_contig81130.18977.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=585bp|location=Sequence derived from alignment at M-pyrifera_M_contig81130:352..936+ (Macrocystis pyrifera P11B4 male)
TACGCGTTGTATGACACGGACGAGCAGCATGGACCTGATCAGTGGCGGTG TTATTCTGCCAGTTCCTTGGATCCTACCCATTCGCACTACGTGAACGGTA GCAGCTACTGCACACGAGAGCAGCAGTTGACGCAGCTGATGGGGAATTGT GCGAAAGGGGTGGAAACCACGATCAACCCAGTATTCATGCACGGAGATGG CGGGTATCCTTGCATCCGCATCCCCTCCATCCTCCAAGCCCCTTCTGGAG TGCTGTTCGCATTCGCTGAATGCCGGCATTGGATCGGAGATGGGTGTGAA CCGGCGGGGCAGAAGGGTGTGGAAGGTGGAACCACAGATGCGTGCATGAA ATCGTCAGGGGACGGAGGCAAGACGTGGGGCAATCTCACCGTCGTGCTGC AAGATGCGTCCCAGCCTTCTCCAACCATGATGCACAACGGAATGCTCAAC CTCCAACTCGACCACCAAGGCAACAGCACGCAAATGCTAGGCACCATCAA AGGAACCTCCATCTCCTTCTCCCCACCCACCTCGCTCGCAAAATACCTCG GACCTGCCAACGGTGCCGCTGTGGGACCAGGGGTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81130:352..936+

>mRNA_M-pyrifera_M_contig81130.18977.1 ID=mRNA_M-pyrifera_M_contig81130.18977.1|Name=mRNA_M-pyrifera_M_contig81130.18977.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1170bp|location=Sequence derived from alignment at M-pyrifera_M_contig81130:352..936+ (Macrocystis pyrifera P11B4 male)
TACGCGTTGTATGACACGGACGAGCAGCATGGACCTGATCAGTGGCGGTG
TTATTCTGCCAGTTCCTTGGATCCTACCCATTCGCACTACGTGAACGGTA
GCAGCTACTGCACACGAGAGCAGCAGTTGACGCAGCTGATGGGGAATTGT
GCGAAAGGGGTGGAAACCACGATCAACCCAGTATTCATGCACGGAGATGG
CGGGTATCCTTGCATCCGCATCCCCTCCATCCTCCAAGCCCCTTCTGGAG
TGCTGTTCGCATTCGCTGAATGCCGGCATTGGATCGGAGATGGGTGTGAA
CCGGCGGGGCAGAAGGGTGTGGAAGGTGGAACCACAGATGCGTGCATGAA
ATCGTCAGGGGACGGAGGCAAGACGTGGGGCAATCTCACCGTCGTGCTGC
AAGATGCGTCCCAGCCTTCTCCAACCATGATGCACAACGGAATGCTCAAC
CTCCAACTCGACCACCAAGGCAACAGCACGCAAATGCTAGGCACCATCAA
AGGAACCTCCATCTCCTTCTCCCCACCCACCTCGCTCGCAAAATACCTCG
GACCTGCCAACGGTGCCGCTGTGGGACCAGGGGTGTACGCGTTGTATGAC
ACGGACGAGCAGCATGGACCTGATCAGTGGCGGTGTTATTCTGCCAGTTC
CTTGGATCCTACCCATTCGCACTACGTGAACGGTAGCAGCTACTGCACAC
GAGAGCAGCAGTTGACGCAGCTGATGGGGAATTGTGCGAAAGGGGTGGAA
ACCACGATCAACCCAGTATTCATGCACGGAGATGGCGGGTATCCTTGCAT
CCGCATCCCCTCCATCCTCCAAGCCCCTTCTGGAGTGCTGTTCGCATTCG
CTGAATGCCGGCATTGGATCGGAGATGGGTGTGAACCGGCGGGGCAGAAG
GGTGTGGAAGGTGGAACCACAGATGCGTGCATGAAATCGTCAGGGGACGG
AGGCAAGACGTGGGGCAATCTCACCGTCGTGCTGCAAGATGCGTCCCAGC
CTTCTCCAACCATGATGCACAACGGAATGCTCAACCTCCAACTCGACCAC
CAAGGCAACAGCACGCAAATGCTAGGCACCATCAAAGGAACCTCCATCTC
CTTCTCCCCACCCACCTCGCTCGCAAAATACCTCGGACCTGCCAACGGTG
CCGCTGTGGGACCAGGGGTG
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