mRNA_M-pyrifera_M_contig79011.18560.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig79011.18560.1
Unique NamemRNA_M-pyrifera_M_contig79011.18560.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: K3X375_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X375_GLOUD)

HSP 1 Score: 122 bits (305), Expect = 6.810e-29
Identity = 71/173 (41.04%), Postives = 108/173 (62.43%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ W  W   P DP T EER E EA  EK R+ EFE  NPEFC+QF+ DLE R+K +KE++R  D+ +++GN L++  +   AL +Y  AL+    ++ VL N++  + R + YD++++F+ RA+F+    +     K+L RRA  W ++    EAA+D+E+A  LDP N DL
Sbjct:  145 YSLWNNW--EPQDPVTQEERAEKEAQLEKQRNAEFESNNPEFCNQFKMDLETRQKTQKEKERIADKLKQQGNQLYKRKQYASALQQYMKALEKCPFNVAVLTNIAQCYLRLEEYDDSVEFSSRALFVDPDHI-----KALSRRAAVWHIQKKWHEAAQDMEKAIQLDPDNEDL 310          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A2D4CHY8_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CHY8_PYTIN)

HSP 1 Score: 120 bits (300), Expect = 3.110e-28
Identity = 69/173 (39.88%), Postives = 114/173 (65.90%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ WE+W+  P+DPAT+EE+ E E   +K R+EEFEK NPEFC QFR+DLE R+K +++R+RD ++ +E GN  ++  +   A+  Y  AL+    ++ VL N++  + R +  D++++F+ RA+F++ + +     K+L RRA  W  +   +EAA D+E+A A+DP N D+
Sbjct:   86 YSVWEQWV--PEDPATLEEKAERERALDKIRNEEFEKNNPEFCQQFREDLEKRQKSQRDRERDAEKLKESGNRCYKKRDYKAAIKFYMNALEKAPFNVAVLTNVAQCYLRLEALDDSVEFSTRALFINPNHV-----KALSRRAAVWHRQQKWKEAAGDMEKALAIDPDNEDV 251          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A2D4BPI2_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BPI2_PYTIN)

HSP 1 Score: 119 bits (298), Expect = 5.710e-28
Identity = 68/173 (39.31%), Postives = 114/173 (65.90%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ W++W+  P+DPAT+EE+ E E   +K R+EEFEK NPEFC QFR+DLE R+K +++R+RD ++ +E GN  ++  +   A+  Y  AL+    ++ VL N++  + R +  D++++F+ RA+F++ + +     K+L RRA  W  +   +EAA D+E+A A+DP N D+
Sbjct:  131 YSVWDQWV--PEDPATLEEKAERERALDKIRNEEFEKNNPEFCQQFREDLEKRQKSQRDRERDAEKLKESGNRCYKKRDYKAAIKFYMNALEKAPFNVAVLTNVAQCYLRLEALDDSVEFSSRALFINPNHV-----KALSRRAAVWHRQQKWKEAAGDMEKALAIDPDNEDV 296          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A0P1A931_PLAHL (Uncharacterized conserved protein, contains LRR repeats n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1A931_PLAHL)

HSP 1 Score: 108 bits (271), Expect = 2.470e-24
Identity = 62/173 (35.84%), Postives = 104/173 (60.12%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ W RW   P+DP T++E+ E E   EK R+ EFE  NP+FC QF++D+++R+K + E+ R  ++ ++RGN  ++  E   A+  Y+ AL     ++ +L N++  + R + YDE ++F  R +FL    +     K+L RRA AW  +   ++AA D+++A  LD  NVD+
Sbjct:  136 YSLWNRW--EPEDPVTLQEKAERENELEKLRNREFETQNPDFCDQFKKDMDERQKSQNEKTRLAEQLKQRGNGFYKKKEYMNAIKNYKDALLASPFNVAILANIAQCYLRLEEYDECIEFCTRTLFLDPKYI-----KALSRRATAWHHQKKFQKAADDMKKALELDKENVDI 301          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A024TQJ5_9STRA (Uncharacterized protein n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TQJ5_9STRA)

HSP 1 Score: 105 bits (263), Expect = 2.910e-23
Identity = 68/177 (38.42%), Postives = 109/177 (61.58%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDP-ANVDLKQE 528
            Y+ W++W   P DP T+EE+   +AL EK +++EFEKANP FC+QF++DLE R+K  ++++R   +H+ +GN  F+  +   AL  Y AAL        +L N++ +H R    D+AL+F  RA+F++   +     K+L R+A    ++GNL+EA   + +A  LDP +N DL Q+
Sbjct:  133 YSMWDKW--EPKDPVTLEEKARQDALVEKMKNDEFEKANPAFCTQFKEDLEKRQKSTRDKERVAQKHKAQGNASFKRRQYAAALTSYMAALVESPYCPLILTNIAQVHLRLHATDDALEFCNRALFVAPTHI-----KALSRKAAILHVRGNLQEAYDLVRKAVDLDPTSNPDLMQQ 302          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: G4ZX90_PHYSP (Uncharacterized protein n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4ZX90_PHYSP)

HSP 1 Score: 105 bits (263), Expect = 2.940e-23
Identity = 59/174 (33.91%), Postives = 109/174 (62.64%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLS-KHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ W++W+  P+DP +++E+ E EA  EK R+ EFE  NPEFC+QF++D+E+R++ ++E+ R  +  +++GN  ++  + ++A+  Y  AL     ++ +L N++  + R  + D+ ++F  R +++  KH       K+L RRA AW  +  L+EAA D+++A  LD  N D+
Sbjct:  138 YSLWDQWV--PEDPVSLQEKAEREAELEKLRNREFETNNPEFCNQFKKDMEERQRSQEEKARTAERLKQKGNRFYKKKQYEDAIKSYMEALLASPFNVAILANIAQCYLRLDQLDDCVEFCTRTLYVDEKHV------KALSRRATAWHRQEKLKEAAEDMKKAFELDGENADV 303          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A2P4Y3V9_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4Y3V9_9STRA)

HSP 1 Score: 103 bits (256), Expect = 5.770e-23
Identity = 58/173 (33.53%), Postives = 104/173 (60.12%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ W +W+  P+DP +++E+ E EA  EK R+ EFE  NPEFC+QF++D+E+R++ ++++ R  +  + +GN  ++  E  +A+  Y  AL     ++ VL N++  + R    D+  +F  R +F+ ++ +     K+L RRA AW  +   +EAA D+ +A AL+  N D+
Sbjct:  136 YSLWNQWV--PEDPVSLQEKAEREAELEKLRNREFENNNPEFCNQFKKDMEERQRSQEQKARMAELLKHKGNRFYKRKEYQDAIKSYMEALTASPFNVAVLANIAQCYLRLDELDDCTEFCTRTLFIDENYV-----KALSRRATAWHRQKRYKEAAEDMRKAFALESENADI 301          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A3R7HVW8_9STRA (Uncharacterized protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7HVW8_9STRA)

HSP 1 Score: 102 bits (253), Expect = 2.290e-22
Identity = 57/173 (32.95%), Postives = 106/173 (61.27%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDL 519
            Y+ W++W+  P+DP +++E+ E EA  EK R+ EFE  NP+FC+QF++D+E R+  ++E++R  +  +++GN  ++  + ++A+  Y  AL     ++ VL N++  + R     + ++F  R +++    +     K+L RRA A+  +  L+EAA D+ +A ALD  NVD+
Sbjct:  136 YSLWDQWV--PEDPVSLQEKAEREAELEKLRNREFENNNPDFCNQFKEDMEKRQHSQEEKERTAERLKQKGNRCYKKKQYEDAIKSYMQALSASPFNVAVLANIAQCYLRLDELSDCIEFCTRTLYIDADYV-----KALSRRATAFHQQKRLKEAADDMRKAFALDKENVDI 301          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: D0NKV1_PHYIT (Uncharacterized protein n=2 Tax=Phytophthora infestans TaxID=4787 RepID=D0NKV1_PHYIT)

HSP 1 Score: 101 bits (252), Expect = 8.730e-22
Identity = 58/176 (32.95%), Postives = 107/176 (60.80%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDLKQE 528
            Y+ W +W+  P+D  +++E+ E  A  EK R+ EFE  NPEFC+QF++D+E+R++ ++E+ R  +  R++GN  ++  +  +A+  Y  AL     ++ VL N++  + R  + D+ ++   R +++ +  +     K+L RRA AW  +  L+EAA D+ +A ALD  NVD+ ++
Sbjct:  136 YSLWNQWV--PEDSVSLQEKAERNAELEKLRNREFEDINPEFCNQFKKDMEERQRSQEEKARTAERLRQKGNHFYKKKQYPDAIKTYMEALKASPFNVAVLANIAQCYLRLDQLDDCVEVCTRTLYVDEGYV-----KALSRRATAWHRQKRLKEAADDMRKAFALDGENVDIAEQ 304          
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Match: A0A329S2Y5_9STRA (Uncharacterized protein n=1 Tax=Phytophthora cactorum TaxID=29920 RepID=A0A329S2Y5_9STRA)

HSP 1 Score: 100 bits (249), Expect = 2.210e-21
Identity = 58/176 (32.95%), Postives = 104/176 (59.09%), Query Frame = 1
Query:    1 YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDLEDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKVLLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLKGNLEEAARDLEQASALDPANVDLKQE 528
            Y+ W +W+  P+DP +++E+ E EA  +K R+ EFE  NPEFC+QF++D+E+R++            +++GN  ++  + +EA+  Y  AL     ++ VL N +  + R  + D+ ++F  R +F+ ++ +     K+L RRA  W  +  L+EAA D+ +A ALD  NVD+ ++
Sbjct:  136 YSLWNQWV--PEDPVSLQEKAEREAELDKLRNREFENTNPEFCNQFKKDMEERQRXXXXXXXXXXXLKQKGNRFYKKKQYEEAIQSYMEALTASPFNVAVLANTAQCYLRLDQLDDCVEFCTRTLFVDENYV-----KALSRRATVWHRQEKLKEAADDMRKAFALDEENVDIAEQ 304          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig79011.18560.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
K3X375_GLOUD6.810e-2941.04Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
A0A2D4CHY8_PYTIN3.110e-2839.88Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A2D4BPI2_PYTIN5.710e-2839.31Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A0P1A931_PLAHL2.470e-2435.84Uncharacterized conserved protein, contains LRR re... [more]
A0A024TQJ5_9STRA2.910e-2338.42Uncharacterized protein n=2 Tax=Aphanomyces invada... [more]
G4ZX90_PHYSP2.940e-2333.91Uncharacterized protein n=1 Tax=Phytophthora sojae... [more]
A0A2P4Y3V9_9STRA5.770e-2333.53Uncharacterized protein (Fragment) n=1 Tax=Phytoph... [more]
A0A3R7HVW8_9STRA2.290e-2232.95Uncharacterized protein n=3 Tax=Phytophthora kerno... [more]
D0NKV1_PHYIT8.730e-2232.95Uncharacterized protein n=2 Tax=Phytophthora infes... [more]
A0A329S2Y5_9STRA2.210e-2132.95Uncharacterized protein n=1 Tax=Phytophthora cacto... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig79011contigM-pyrifera_M_contig79011:431..958 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score125.2
Seed ortholog evalue3.1e-26
Seed eggNOG ortholog65071.PYU1_T011674
Preferred nameycf3
Hectar predicted targeting categoryother localisation
EggNOG free text desc.TPR repeat
EggNOG OGs1MAC4@121069,COG0457@1,KOG1124@2759
COG Functional cat.S
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
Exons1
Model size528
Cds size528
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816693.2569268-CDS-M-pyrifera_M_contig79011:430..9581622816693.2569268-CDS-M-pyrifera_M_contig79011:430..958Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig79011 431..958 -
1692278123.4363127-CDS-M-pyrifera_M_contig79011:430..9581692278123.4363127-CDS-M-pyrifera_M_contig79011:430..958Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig79011 431..958 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig79011.18560.1prot_M-pyrifera_M_contig79011.18560.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig79011 431..958 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig79011.18560.1

>prot_M-pyrifera_M_contig79011.18560.1 ID=prot_M-pyrifera_M_contig79011.18560.1|Name=mRNA_M-pyrifera_M_contig79011.18560.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=176bp
YARWERWLENPDDPATMEEREELEALQEKARDEEFEKANPEFCSQFRQDL
EDRKKREKERQRDCDEHRERGNDLFRGGELDEALGEYEAALDLHKLDLKV
LLNLSALHARAKRYDEALDFAERAVFLSKHTLGNPTPKSLFRRANAWKLK
GNLEEAARDLEQASALDPANVDLKQE
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mRNA from alignment at M-pyrifera_M_contig79011:431..958-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig79011.18560.1 ID=mRNA_M-pyrifera_M_contig79011.18560.1|Name=mRNA_M-pyrifera_M_contig79011.18560.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=528bp|location=Sequence derived from alignment at M-pyrifera_M_contig79011:431..958- (Macrocystis pyrifera P11B4 male)
TATGCTCGATGGGAACGATGGTTGGAAAACCCTGACGACCCTGCAACAAT GGAGGAAAGAGAGGAGTTGGAAGCGTTGCAAGAAAAAGCCAGAGATGAAG AATTCGAAAAGGCAAACCCTGAATTTTGCTCCCAGTTTCGACAAGATCTC GAAGACAGAAAGAAAAGGGAAAAAGAGAGACAACGAGATTGCGATGAGCA TCGAGAAAGAGGAAACGATCTCTTCCGCGGAGGGGAGCTGGACGAGGCAT TGGGAGAGTACGAAGCTGCGTTGGATTTGCACAAGCTGGACCTCAAAGTA CTCCTCAACCTGTCCGCACTCCACGCAAGAGCGAAGAGGTACGACGAAGC GCTCGATTTCGCAGAGCGGGCCGTCTTCCTTTCCAAACACACTCTAGGCA ACCCGACACCAAAGTCACTGTTTCGAAGAGCAAATGCGTGGAAACTCAAA GGGAACCTAGAAGAAGCAGCTCGTGATTTGGAGCAAGCCTCAGCCCTTGA TCCTGCAAATGTGGATCTCAAGCAGGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig79011:431..958-

>mRNA_M-pyrifera_M_contig79011.18560.1 ID=mRNA_M-pyrifera_M_contig79011.18560.1|Name=mRNA_M-pyrifera_M_contig79011.18560.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1056bp|location=Sequence derived from alignment at M-pyrifera_M_contig79011:431..958- (Macrocystis pyrifera P11B4 male)
TATGCTCGATGGGAACGATGGTTGGAAAACCCTGACGACCCTGCAACAAT
GGAGGAAAGAGAGGAGTTGGAAGCGTTGCAAGAAAAAGCCAGAGATGAAG
AATTCGAAAAGGCAAACCCTGAATTTTGCTCCCAGTTTCGACAAGATCTC
GAAGACAGAAAGAAAAGGGAAAAAGAGAGACAACGAGATTGCGATGAGCA
TCGAGAAAGAGGAAACGATCTCTTCCGCGGAGGGGAGCTGGACGAGGCAT
TGGGAGAGTACGAAGCTGCGTTGGATTTGCACAAGCTGGACCTCAAAGTA
CTCCTCAACCTGTCCGCACTCCACGCAAGAGCGAAGAGGTACGACGAAGC
GCTCGATTTCGCAGAGCGGGCCGTCTTCCTTTCCAAACACACTCTAGGCA
ACCCGACACCAAAGTCACTGTTTCGAAGAGCAAATGCGTGGAAACTCAAA
GGGAACCTAGAAGAAGCAGCTCGTGATTTGGAGCAAGCCTCAGCCCTTGA
TCCTGCAAATGTGGATCTCAAGCAGGAGTATGCTCGATGGGAACGATGGT
TGGAAAACCCTGACGACCCTGCAACAATGGAGGAAAGAGAGGAGTTGGAA
GCGTTGCAAGAAAAAGCCAGAGATGAAGAATTCGAAAAGGCAAACCCTGA
ATTTTGCTCCCAGTTTCGACAAGATCTCGAAGACAGAAAGAAAAGGGAAA
AAGAGAGACAACGAGATTGCGATGAGCATCGAGAAAGAGGAAACGATCTC
TTCCGCGGAGGGGAGCTGGACGAGGCATTGGGAGAGTACGAAGCTGCGTT
GGATTTGCACAAGCTGGACCTCAAAGTACTCCTCAACCTGTCCGCACTCC
ACGCAAGAGCGAAGAGGTACGACGAAGCGCTCGATTTCGCAGAGCGGGCC
GTCTTCCTTTCCAAACACACTCTAGGCAACCCGACACCAAAGTCACTGTT
TCGAAGAGCAAATGCGTGGAAACTCAAAGGGAACCTAGAAGAAGCAGCTC
GTGATTTGGAGCAAGCCTCAGCCCTTGATCCTGCAAATGTGGATCTCAAG
CAGGAG
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