mRNA_M-pyrifera_M_contig78649.18483.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig78649.18483.1
Unique NamemRNA_M-pyrifera_M_contig78649.18483.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A7V8V8T7_9BACT (Uncharacterized protein n=2 Tax=Bremerella alba TaxID=980252 RepID=A0A7V8V8T7_9BACT)

HSP 1 Score: 464 bits (1193), Expect = 8.550e-158
Identity = 223/225 (99.11%), Postives = 224/225 (99.56%), Query Frame = 1
Query:    1 DFRSNVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYVAAHDFAIYDNTFTNSHGVSFPASCRTLTANAPIVVSPTSSNIDFHGEDIDPETLDQFSEQDWDDCVPNWPL 675
            DFRSNVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVT+SNPTQAEIYNNTVINTVNGMYVAAHDFAIYDNTFTNSHGVSFPASCRTLTANAPIVVSPTSSNIDFHGEDIDPETL QFSEQDWDDCVPNWPL
Sbjct:  526 DFRSNVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTLSNPTQAEIYNNTVINTVNGMYVAAHDFAIYDNTFTNSHGVSFPASCRTLTANAPIVVSPTSSNIDFHGEDIDPETLYQFSEQDWDDCVPNWPL 750          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A7V4LG25_9DELT (Right-handed parallel beta-helix repeat-containing protein n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A7V4LG25_9DELT)

HSP 1 Score: 245 bits (625), Expect = 1.660e-75
Identity = 117/223 (52.47%), Postives = 148/223 (66.37%), Query Frame = 1
Query:   10 SNVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYVAAHDFAIYDNTFTNSHGVSFPASC-RTLTANAPIVVSPTSSNIDFHGEDIDPETLDQFSEQDWDDCVPNWPL 675
            S V + D+ FAYNG H    +W+DGLTVHD   S F GN FIDNTDID IFGG +   + NNTV+HT D  GGAFA+IMIHKWP+TSG Y    +  NSVD GP +  G+GLYIASEGWY ETP+G  +   T   I  N V+N +N +Y+AA   AIY N F N+HGVS P SC R++ +  PIVVSPT+++  F+ E++DP     +S   W  C+PN+P 
Sbjct:  270 SGVAIRDSTFAYNGRHTTPNMWSDGLTVHDPSRSDFSGNTFIDNTDIDLIFGGCRACTVRNNTVLHTDDPAGGAFAAIMIHKWPSTSGCYEDVDVSGNSVDCGPRRACGSGLYIASEGWYPETPYGTLVDGTTSGLITRNAVVNAMNALYIAARGLAIYGNDFLNAHGVSIPNSCHRSIVSVTPIVVSPTTASCHFNYENVDPVMSVHYSSASWAGCIPNYPF 492          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A7X9P942_9DELT (Right-handed parallel beta-helix repeat-containing protein n=1 Tax=Myxococcales bacterium TaxID=2026763 RepID=A0A7X9P942_9DELT)

HSP 1 Score: 243 bits (621), Expect = 8.890e-75
Identity = 120/221 (54.30%), Postives = 146/221 (66.06%), Query Frame = 1
Query:   16 VRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYVAAHDFAIYDNTFTNSHGVSFPASC-RTLTANAPIVVSPTSSNIDFHGEDIDPETLDQFSEQDWDDCVPNWPL 675
            V + D+ FAYNG H    LW+DGLTVHD   S F GN FIDNTDID IFGG +   I NNTV HT D  GGAFA+IMIHKWPTTSG Y    +  NSVD GP +  G+GLYIASEGWY ETP+G  +   T   I  N V+N +N +Y+AA   AIY N F N+HGVS P SC R++ +  PIVVSPT+++  F+ E+IDP     +S   W  C+PN+P 
Sbjct:  284 VAIRDSTFAYNGRHTVRNLWSDGLTVHDPSRSDFSGNTFIDNTDIDLIFGGCRACTIRNNTVRHTDDPAGGAFAAIMIHKWPTTSGCYEDVDVSGNSVDCGPLRACGSGLYIASEGWYPETPYGTLVDGTTSGLITRNAVVNAMNALYIAARGLAIYGNDFLNAHGVSIPNSCHRSIVSVTPIVVSPTTASCHFNYENIDPVMSVHYSSASWAGCIPNYPF 504          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A1V5XBM4_9DELT (Uncharacterized protein n=1 Tax=Deltaproteobacteria bacterium ADurb.Bin207 TaxID=1852869 RepID=A0A1V5XBM4_9DELT)

HSP 1 Score: 214 bits (546), Expect = 1.630e-63
Identity = 105/222 (47.30%), Postives = 135/222 (60.81%), Query Frame = 1
Query:   13 NVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYVAAHDFAIYDNTFTNSHGVSFPASC-RTLTANAPIVVSPTSSNIDFHGEDIDPETLDQFSEQDWDDCVPNWPL 675
            NV +  N FA+NG+H    +W+DGLTVHD+    +  N FIDNTDID IFGG +   I  N V HT+D  GGAFA+IMI KWPTTSG Y    I  N+VD GP +  G+  YI SE WY ETP+G   +  T   I  N+V   +N  Y+AA   AIY N F  +HGV  P SC + + +  PIVVSPT+++  F+ E++DP     +S   W  CVPN+P 
Sbjct:  293 NVTISHNTFAFNGLHTTKNMWSDGLTVHDSEKGVYEHNTFIDNTDIDLIFGGCKKCKIRYNQVHHTSDPAGGAFAAIMIQKWPTTSGCYEDVDISGNAVDCGPLRACGSAFYIGSESWYPETPYGTLQNGTTSGLIKGNSVNRAMNAFYIAARGLAIYGNDFLQAHGVPIPNSCHKNIVSTTPIVVSPTAASCHFNFENVDPGMSIHYSSASWAGCVPNYPF 514          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A3M1CQX0_9DELT (N_BRCA1_IG domain-containing protein n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A3M1CQX0_9DELT)

HSP 1 Score: 164 bits (416), Expect = 2.510e-43
Identity = 90/222 (40.54%), Postives = 127/222 (57.21%), Query Frame = 1
Query:    7 RSNVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYVA-AHDFAIYDNTFTNSHGVSFPASCRTLTANAPIVVSPTSSNIDFHGEDIDPETLDQFSEQDWDDCVPNW 669
            R+ V V ++     GVH++  LW+DG+TVHDA +S+F GN   DNTDID IFGG +N  I  NT++H    +  +FA++MIH WPTTSGNY GT++ +N++D G  K  G GLYI  + WYD   FG          ++ NTV N   G+ +  AHD  +YDN  + + G++  ASC   T      +   S NID   + I       + + D+D C+PNW
Sbjct:  540 RARVVVSNSTVVGCGVHDREGLWSDGITVHDATDSRFTGNYLADNTDIDLIFGGCRNCDISGNTILHGHAFSSSSFAALMIHAWPTTSGNYQGTVVHDNTIDCGAQKQCGIGLYIGPDAWYDADTFG--------GSVHGNTVKNAQFGLLIDDAHDVEVYDNPVSGTKGLT-KASCGWKTT-YDYGIGRDSFNIDTSSDSIGAV----YHQVDFDRCIPNW 747          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A4P6XC58_9SPHN (Right-handed parallel beta-helix repeat-containing protein n=1 Tax=Sphingomonas sp. AAP5 TaxID=1523415 RepID=A0A4P6XC58_9SPHN)

HSP 1 Score: 119 bits (297), Expect = 5.380e-28
Identity = 67/177 (37.85%), Postives = 95/177 (53.67%), Query Frame = 1
Query:   16 VRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYV--AAHDFAIYDNTFTNSHGVSFPASCRT 540
            +RV D++F  NG H    +WADGLTVH+A ++    NLF DNTD+  IFGG     I +N+  H+    G +FA +M+  WPTTSG++SG++I  N+++  P K  G G+ I S  WY            T  E+  NTV   + G+ V        I DNT   S G  + ++C T
Sbjct:  196 LRVIDSLFWENGDHQTPLMWADGLTVHEAKDATITRNLFRDNTDVQLIFGGCARCRISHNSFTHSEGFGGASFAELMLQSWPTTSGDFSGSLISENAINCSPTKRCGYGIMIGSSPWY--------AGRATGGEVSRNTVRFAMIGLNVDRLTGPMVIRDNTIAESGG-RYSSACGT 363          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A196MK04_9SPHN (Beta_helix domain-containing protein n=2 Tax=Sphingobium sp. TCM1 TaxID=453246 RepID=A0A196MK04_9SPHN)

HSP 1 Score: 116 bits (291), Expect = 8.060e-28
Identity = 69/197 (35.03%), Postives = 101/197 (51.27%), Query Frame = 1
Query:   16 VRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWP-TTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNG-MYVAAHDFAIYDNTFTNSHGVSFPASCRT--LTANAPIVVSPTSSNIDF 594
            V + DN+F+ NG HN    WADGLT+H A      GN FIDN+D+  IFG   +  I +N   H+    GG+FA +M+H WP +TSG+++GT +  N +D G  +  G G+ I S+ WYD   FG T+S            I+T+ G M V  +D  +    + +  GV      R     A+ P V    + ++ F
Sbjct:   97 VVIRDNLFSRNGRHNVQSHWADGLTIHTARRFAVTGNRFIDNSDVQLIFGSCVDCTISDNRFDHSGSAEGGSFAELMLHAWPGSTSGDFTGTRVTRNRIDCGQQQRCGFGIMIGSKPWYDAPAFGGTVSGNRVRGAMIALNIDTLTGPMTVEDNDLNLSSGRYPSMCGVQDVRGIRANISPASRPFVAGMPARDLSF 293          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: A0A653J7A1_9SPHN (Beta_helix domain-containing protein n=1 Tax=Novosphingobium sp. 9U TaxID=2653158 RepID=A0A653J7A1_9SPHN)

HSP 1 Score: 115 bits (289), Expect = 6.820e-27
Identity = 51/128 (39.84%), Postives = 78/128 (60.94%), Query Frame = 1
Query:   16 VRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMS 399
            + V D++   NG H+   +WADG+TVHDA ++    N F+DNTD+  IFGG    +++NN+  H++     +FA +M+H W  TSGN+SG++   N +D G ++  G G+ I  E WY    FG T+S
Sbjct:  189 LSVTDSLIGPNGQHDTLMMWADGITVHDAASAVIQNNTFLDNTDVQLIFGGCTECIVKNNSFRHSSSFKRASFAELMLHAWSNTSGNFSGSVTSQNKIDCGRSRRCGYGIMIGGEPWYPAKTFGGTVS 316          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: UPI001F5A4C1B (hypothetical protein n=1 Tax=Sphingomonas sp. BAUL-RG-20F-R05-02 TaxID=2914830 RepID=UPI001F5A4C1B)

HSP 1 Score: 113 bits (282), Expect = 6.130e-26
Identity = 63/192 (32.81%), Postives = 99/192 (51.56%), Query Frame = 1
Query:   13 NVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWP-TTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVNGMYVAA--HDFAIYDNTFTNSHGVSFPASCRTLTANAPIVVSPTS 579
            N  +  N  A NG H    +W+DGLTVHDA +   V N FIDNTD+  IFGG  + ++ NN   H   + GG++A +M+  WP +TSG Y GT + NN ++ GP +  G GL + S  WYD    G  +S         N ++N V  + +     +  + +N+ + +      ++C  L  ++ + V+  S
Sbjct:  175 NGHIFGNTIAGNGTHTVHLMWSDGLTVHDAQHLAVVHNHFIDNTDVQLIFGGCTHCIVANNIFRHGGVSAGGSYAELMVQAWPKSTSGRYDGTKVVNNDINCGPQRDCGFGLMLGSTPWYDAPTSGGVVSG--------NRIVNAVLPIDIEGLTGEMTVTNNSISGAPAGRVRSTCGLLNVSSQVNVAAAS 358          
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Match: F6IC46_9SPHN (Beta_helix domain-containing protein n=2 Tax=Novosphingobium sp. PP1Y TaxID=702113 RepID=F6IC46_9SPHN)

HSP 1 Score: 110 bits (276), Expect = 4.640e-25
Identity = 49/119 (41.18%), Postives = 74/119 (62.18%), Query Frame = 1
Query:   46 NGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDIDFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRNNSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSN 402
            NG H+   +WADG+T+HD+  +    N F+DNTD+  +FGG +N ++E NT  H+A     +FA +M+H WP TSG+++G+II  N++D    +  G G+ I  E WY    FG  +SN
Sbjct:  195 NGTHDVHQMWADGVTIHDSSGAYVKNNEFLDNTDVQLVFGGCRNCIVEGNTFHHSAAFAHASFAELMLHAWPNTSGDFTGSIISLNNIDCNKARRCGYGIMIGGEPWYPARTFGGIVSN 313          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig78649.18483.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 16
Match NameE-valueIdentityDescription
A0A7V8V8T7_9BACT8.550e-15899.11Uncharacterized protein n=2 Tax=Bremerella alba Ta... [more]
A0A7V4LG25_9DELT1.660e-7552.47Right-handed parallel beta-helix repeat-containing... [more]
A0A7X9P942_9DELT8.890e-7554.30Right-handed parallel beta-helix repeat-containing... [more]
A0A1V5XBM4_9DELT1.630e-6347.30Uncharacterized protein n=1 Tax=Deltaproteobacteri... [more]
A0A3M1CQX0_9DELT2.510e-4340.54N_BRCA1_IG domain-containing protein n=1 Tax=Delta... [more]
A0A4P6XC58_9SPHN5.380e-2837.85Right-handed parallel beta-helix repeat-containing... [more]
A0A196MK04_9SPHN8.060e-2835.03Beta_helix domain-containing protein n=2 Tax=Sphin... [more]
A0A653J7A1_9SPHN6.820e-2739.84Beta_helix domain-containing protein n=1 Tax=Novos... [more]
UPI001F5A4C1B6.130e-2632.81hypothetical protein n=1 Tax=Sphingomonas sp. BAUL... [more]
F6IC46_9SPHN4.640e-2541.18Beta_helix domain-containing protein n=2 Tax=Novos... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig78649contigM-pyrifera_M_contig78649:26..703 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score84.0
Seed ortholog evalue1e-13
Seed eggNOG ortholog1242864.D187_009424
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Ig-like domain from next to BRCA1 gene
EggNOG OGs1Q3W1@1224,2X51Z@28221,2Z002@29,439Q0@68525,COG3209@1,COG3209@2
COG Functional cat.M
Best tax levelMyxococcales
Best eggNOG OGNA|NA|NA
Exons1
Model size678
Cds size678
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816689.3082933-CDS-M-pyrifera_M_contig78649:25..7031622816689.3082933-CDS-M-pyrifera_M_contig78649:25..703Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78649 26..703 +
1692278121.1702077-CDS-M-pyrifera_M_contig78649:25..7031692278121.1702077-CDS-M-pyrifera_M_contig78649:25..703Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78649 26..703 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig78649.18483.1prot_M-pyrifera_M_contig78649.18483.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig78649 26..703 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig78649.18483.1

>prot_M-pyrifera_M_contig78649.18483.1 ID=prot_M-pyrifera_M_contig78649.18483.1|Name=mRNA_M-pyrifera_M_contig78649.18483.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=226bp
DFRSNVRVDDNIFAYNGVHNQSFLWADGLTVHDAYNSQFVGNLFIDNTDI
DFIFGGAQNSLIENNTVIHTADTTGGAFASIMIHKWPTTSGNYSGTIIRN
NSVDGGPNKTAGTGLYIASEGWYDETPFGVTMSNPTQAEIYNNTVINTVN
GMYVAAHDFAIYDNTFTNSHGVSFPASCRTLTANAPIVVSPTSSNIDFHG
EDIDPETLDQFSEQDWDDCVPNWPL*
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mRNA from alignment at M-pyrifera_M_contig78649:26..703+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig78649.18483.1 ID=mRNA_M-pyrifera_M_contig78649.18483.1|Name=mRNA_M-pyrifera_M_contig78649.18483.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=678bp|location=Sequence derived from alignment at M-pyrifera_M_contig78649:26..703+ (Macrocystis pyrifera P11B4 male)
GATTTTCGTAGCAACGTGAGGGTCGACGACAATATCTTTGCCTACAACGG CGTACACAACCAATCGTTCCTTTGGGCCGATGGCCTTACCGTTCACGATG CCTACAACTCGCAGTTTGTTGGCAATCTGTTCATCGACAACACCGATATC GACTTCATCTTTGGTGGTGCTCAAAATTCTCTGATCGAAAACAATACCGT CATCCACACGGCTGATACGACAGGCGGCGCTTTTGCCTCGATCATGATTC ATAAGTGGCCGACAACCTCGGGGAACTATTCTGGGACAATCATTCGAAAC AACTCCGTCGATGGTGGCCCCAACAAAACAGCAGGGACAGGTCTCTACAT CGCCTCTGAAGGTTGGTACGACGAAACACCCTTTGGCGTGACCATGAGCA ACCCCACGCAGGCAGAGATCTACAACAACACCGTCATCAACACGGTCAAC GGTATGTATGTCGCCGCGCATGATTTTGCGATCTACGACAACACATTTAC CAACAGCCATGGTGTGAGCTTCCCTGCCTCTTGCCGAACCCTTACCGCGA ATGCGCCGATTGTCGTCTCACCGACATCGAGCAACATCGACTTTCACGGC GAAGATATCGATCCCGAAACATTGGACCAGTTCTCAGAGCAAGACTGGGA CGACTGCGTACCGAACTGGCCTTTATAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig78649:26..703+

>mRNA_M-pyrifera_M_contig78649.18483.1 ID=mRNA_M-pyrifera_M_contig78649.18483.1|Name=mRNA_M-pyrifera_M_contig78649.18483.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1356bp|location=Sequence derived from alignment at M-pyrifera_M_contig78649:26..703+ (Macrocystis pyrifera P11B4 male)
GATTTTCGTAGCAACGTGAGGGTCGACGACAATATCTTTGCCTACAACGG
CGTACACAACCAATCGTTCCTTTGGGCCGATGGCCTTACCGTTCACGATG
CCTACAACTCGCAGTTTGTTGGCAATCTGTTCATCGACAACACCGATATC
GACTTCATCTTTGGTGGTGCTCAAAATTCTCTGATCGAAAACAATACCGT
CATCCACACGGCTGATACGACAGGCGGCGCTTTTGCCTCGATCATGATTC
ATAAGTGGCCGACAACCTCGGGGAACTATTCTGGGACAATCATTCGAAAC
AACTCCGTCGATGGTGGCCCCAACAAAACAGCAGGGACAGGTCTCTACAT
CGCCTCTGAAGGTTGGTACGACGAAACACCCTTTGGCGTGACCATGAGCA
ACCCCACGCAGGCAGAGATCTACAACAACACCGTCATCAACACGGTCAAC
GGTATGTATGTCGCCGCGCATGATTTTGCGATCTACGACAACACATTTAC
CAACAGCCATGGTGTGAGCTTCCCTGCCTCTTGCCGAACCCTTACCGCGA
ATGCGCCGATTGTCGTCTCACCGACATCGAGCAACATCGACTTTCACGGC
GAAGATATCGATCCCGAAACATTGGACCAGTTCTCAGAGCAAGACTGGGA
CGACTGCGTACCGAACTGGCCTTTATAGGATTTTCGTAGCAACGTGAGGG
TCGACGACAATATCTTTGCCTACAACGGCGTACACAACCAATCGTTCCTT
TGGGCCGATGGCCTTACCGTTCACGATGCCTACAACTCGCAGTTTGTTGG
CAATCTGTTCATCGACAACACCGATATCGACTTCATCTTTGGTGGTGCTC
AAAATTCTCTGATCGAAAACAATACCGTCATCCACACGGCTGATACGACA
GGCGGCGCTTTTGCCTCGATCATGATTCATAAGTGGCCGACAACCTCGGG
GAACTATTCTGGGACAATCATTCGAAACAACTCCGTCGATGGTGGCCCCA
ACAAAACAGCAGGGACAGGTCTCTACATCGCCTCTGAAGGTTGGTACGAC
GAAACACCCTTTGGCGTGACCATGAGCAACCCCACGCAGGCAGAGATCTA
CAACAACACCGTCATCAACACGGTCAACGGTATGTATGTCGCCGCGCATG
ATTTTGCGATCTACGACAACACATTTACCAACAGCCATGGTGTGAGCTTC
CCTGCCTCTTGCCGAACCCTTACCGCGAATGCGCCGATTGTCGTCTCACC
GACATCGAGCAACATCGACTTTCACGGCGAAGATATCGATCCCGAAACAT
TGGACCAGTTCTCAGAGCAAGACTGGGACGACTGCGTACCGAACTGGCCT
TTATAG
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