mRNA_M-pyrifera_M_contig78628.18477.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig78628.18477.1
Unique NamemRNA_M-pyrifera_M_contig78628.18477.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: D8LNY9_ECTSI (DNA repair helicase rad5,16, putative (Partial) (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNY9_ECTSI)

HSP 1 Score: 104 bits (259), Expect = 5.420e-28
Identity = 51/62 (82.26%), Postives = 58/62 (93.55%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNL 189
            ++WTHMLDLAE S+ARGGWTFRRLDGAMSQQ+RES+LK F TD SV+V+LVSLKAGGVGLNL
Sbjct:   14 SQWTHMLDLAEASIARGGWTFRRLDGAMSQQQRESALKGFATDESVTVMLVSLKAGGVGLNL 75          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A6H5KHD4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KHD4_9PHAE)

HSP 1 Score: 107 bits (267), Expect = 1.270e-25
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLTA 195
            ++WTHMLDLAE S+ARGGWTFRRLDGAMSQQ+RES+LK F TD SV+V+LVSLKAGGVGLNLTA
Sbjct: 1470 SQWTHMLDLAEASIARGGWTFRRLDGAMSQQQRESALKGFATDESVTVMLVSLKAGGVGLNLTA 1533          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A6V1XFX6_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1XFX6_HETAK)

HSP 1 Score: 78.2 bits (191), Expect = 2.090e-16
Identity = 37/63 (58.73%), Postives = 49/63 (77.78%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLT 192
            ++WT+MLDL E  + R GW   RLDG+MSQQ+RE +L+ F T+  + V+L+SLKAGGVGLNLT
Sbjct:   47 SQWTYMLDLVEVLLRRMGWRHTRLDGSMSQQQREDALERFATETDIPVMLLSLKAGGVGLNLT 109          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A0L0H763_SPIPD (Uncharacterized protein n=2 Tax=Spizellomyces TaxID=4815 RepID=A0A0L0H763_SPIPD)

HSP 1 Score: 75.5 bits (184), Expect = 2.150e-14
Identity = 39/63 (61.90%), Postives = 50/63 (79.37%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLT 192
            ++WT+MLDL E S+   G+ F RLDG MS+Q R  +++SF TD SV+V+LVSLKAGGVGLNLT
Sbjct: 1110 SQWTNMLDLLEPSLRTYGFGFVRLDGKMSRQDRTDAMESFKTDPSVTVMLVSLKAGGVGLNLT 1172          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: SM3L2_ARATH (DNA repair protein RAD5A n=19 Tax=Brassicaceae TaxID=3700 RepID=SM3L2_ARATH)

HSP 1 Score: 73.2 bits (178), Expect = 1.400e-13
Identity = 36/64 (56.25%), Postives = 48/64 (75.00%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLTA 195
            ++WT  LDL +  ++R  ++F RLDG +SQQ+RE  LK F  D S+ VLL+SLKAGGVG+NLTA
Sbjct:  886 SQWTAFLDLLQIPLSRNNFSFVRLDGTLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTA 949          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: UPI0012630830 (DNA repair protein RAD5A isoform X1 n=3 Tax=Pistacia vera TaxID=55513 RepID=UPI0012630830)

HSP 1 Score: 73.2 bits (178), Expect = 1.400e-13
Identity = 37/64 (57.81%), Postives = 47/64 (73.44%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLTA 195
            ++WT  LDL +  ++R   TF RLDG ++QQRRE  LK F  D +V VLL+SLKAGGVG+NLTA
Sbjct:  890 SQWTAFLDLLQIPLSRNNITFLRLDGTLTQQRREKVLKQFSEDTNVLVLLMSLKAGGVGINLTA 953          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A485LGA6_9STRA (RanBP-type and C3HC4-type zinc finger-containing protein 1 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LGA6_9STRA)

HSP 1 Score: 72.8 bits (177), Expect = 1.910e-13
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLTA 195
            ++WTHMLDL E+++ + G++  R DG++ Q+ RE  L  F+ D +V VL++SLKAGGVGLNLTA
Sbjct:  835 SQWTHMLDLVESTLVQHGYSHARFDGSLKQEDRERVLNRFNADPAVRVLVISLKAGGVGLNLTA 898          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A0K9RQK1_SPIOL (Uncharacterized protein n=3 Tax=Chenopodioideae TaxID=1307796 RepID=A0A0K9RQK1_SPIOL)

HSP 1 Score: 72.0 bits (175), Expect = 3.560e-13
Identity = 37/64 (57.81%), Postives = 46/64 (71.88%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLTA 195
            ++WT  LDL E  + R   +F RLDG +SQQ+RE  LK F  D+ V VLL+SLKAGGVG+NLTA
Sbjct:  887 SQWTAFLDLLEIPLTRNNISFLRLDGTLSQQQRERVLKKFSEDSDVMVLLMSLKAGGVGINLTA 950          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A484N638_9ASTE (Uncharacterized protein n=3 Tax=Cuscuta sect. Cleistogrammica TaxID=1824901 RepID=A0A484N638_9ASTE)

HSP 1 Score: 72.0 bits (175), Expect = 3.560e-13
Identity = 36/64 (56.25%), Postives = 49/64 (76.56%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLTA 195
            ++WT  LDL + +++R G +F RLDG ++QQ RE  +K+F  + SV VLL+SLKAGGVGLNLTA
Sbjct:  910 SQWTAFLDLLQIALSRNGISFLRLDGTLNQQHREKVIKTFSEEHSVLVLLMSLKAGGVGLNLTA 973          
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Match: A0A6B2LL46_9EUKA (Helicase C-terminal domain-containing protein (Fragment) n=1 Tax=Arcella intermedia TaxID=1963864 RepID=A0A6B2LL46_9EUKA)

HSP 1 Score: 69.3 bits (168), Expect = 3.850e-13
Identity = 32/63 (50.79%), Postives = 48/63 (76.19%), Query Frame = 1
Query:    4 TKWTHMLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKAGGVGLNLT 192
            ++WT MLDL E  +   G  F RLDG+M+Q  R+++++SF+TD ++ + LVS+KAGG+GLNLT
Sbjct:   33 SQWTTMLDLLEKPLKARGHQFVRLDGSMAQNHRQTAIESFNTDPNIKIFLVSMKAGGLGLNLT 95          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig78628.18477.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LNY9_ECTSI5.420e-2882.26DNA repair helicase rad5,16, putative (Partial) (F... [more]
A0A6H5KHD4_9PHAE1.270e-2582.81Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6V1XFX6_HETAK2.090e-1658.73Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A0L0H763_SPIPD2.150e-1461.90Uncharacterized protein n=2 Tax=Spizellomyces TaxI... [more]
SM3L2_ARATH1.400e-1356.25DNA repair protein RAD5A n=19 Tax=Brassicaceae Tax... [more]
UPI00126308301.400e-1357.81DNA repair protein RAD5A isoform X1 n=3 Tax=Pistac... [more]
A0A485LGA6_9STRA1.910e-1353.13RanBP-type and C3HC4-type zinc finger-containing p... [more]
A0A0K9RQK1_SPIOL3.560e-1357.81Uncharacterized protein n=3 Tax=Chenopodioideae Ta... [more]
A0A484N638_9ASTE3.560e-1356.25Uncharacterized protein n=3 Tax=Cuscuta sect. Clei... [more]
A0A6B2LL46_9EUKA3.850e-1350.79Helicase C-terminal domain-containing protein (Fra... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig78628contigM-pyrifera_M_contig78628:161..820 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score96.7
Seed ortholog evalue3.9e-18
Seed eggNOG ortholog2880.D8LNY9
KEGG koko:K15505
Hectar predicted targeting categoryno signal peptide or anchor
EggNOG free text desc.helicase activity
EggNOG OGsCOG0553@1,KOG1001@2759
COG Functional cat.KL
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03400
Exons2
Model size195
Cds size177
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig78628.18477.1prot_M-pyrifera_M_contig78628.18477.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig78628 179..820 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816688.8977332-UTR-M-pyrifera_M_contig78628:160..1781622816688.8977332-UTR-M-pyrifera_M_contig78628:160..178Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig78628 161..178 +
1692278120.8801255-UTR-M-pyrifera_M_contig78628:160..1781692278120.8801255-UTR-M-pyrifera_M_contig78628:160..178Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig78628 161..178 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816688.9102294-CDS-M-pyrifera_M_contig78628:178..2771622816688.9102294-CDS-M-pyrifera_M_contig78628:178..277Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78628 179..277 +
1692278120.89799-CDS-M-pyrifera_M_contig78628:178..2771692278120.89799-CDS-M-pyrifera_M_contig78628:178..277Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78628 179..277 +
1622816688.925676-CDS-M-pyrifera_M_contig78628:742..8201622816688.925676-CDS-M-pyrifera_M_contig78628:742..820Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78628 743..820 +
1692278120.908273-CDS-M-pyrifera_M_contig78628:742..8201692278120.908273-CDS-M-pyrifera_M_contig78628:742..820Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78628 743..820 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig78628.18477.1

>prot_M-pyrifera_M_contig78628.18477.1 ID=prot_M-pyrifera_M_contig78628.18477.1|Name=mRNA_M-pyrifera_M_contig78628.18477.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=59bp
MLDLAETSMARGGWTFRRLDGAMSQQRRESSLKSFDTDASVSVLLVSLKA
GGVGLNLTA
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mRNA from alignment at M-pyrifera_M_contig78628:161..820+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig78628.18477.1 ID=mRNA_M-pyrifera_M_contig78628.18477.1|Name=mRNA_M-pyrifera_M_contig78628.18477.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=660bp|location=Sequence derived from alignment at M-pyrifera_M_contig78628:161..820+ (Macrocystis pyrifera P11B4 male)
CTTACAAAGTGGACTCATATGCTCGATTTAGCAGAGACGTCTATGGCTAG GGGGGGCTGGACCTTTAGGCGTCTGGACGGTGCAATGAGCCAGCAGAGAA GGGAGTCTTCTCTCAAGGTGAGATAGTCTATAGCCTAAGTCTAGCCAGTC AAACTGAACCACAATACAAACCAAGCCAAATCAAATAATTTCCAAAACCA AACCGAACCAATCCGAACGCAAAGCAAACCGAAATGAACCGAACCGAACC GAATCAACCGTATCCTTATCCTAACCTAGCCCAACATATCCTAACCGGAA TTGACCAGACCTGACCGAACCCAGCTTAACCTAACCTAAACCAACCGAAA GTAACCTGACCGAATCGAACCTAACCCAACCCAGCCCAGTCCATCCCAAC CCAACCCAACCTAGCCTTGATTACTATATTCGCCGCATCTTCCCCCCCCA CCCCCCTCCCTTTTACCCCGCCGGCTCGCGTCACGTTAACTGTGTGCGAT TTGTCCGTTCGGTCATCGTCGCCACCTCTGCCGCCACTACCGCCGCCGCC GCCACCGCCACCGCCACCACCGCCCCCGGCAGAGCTTCGACACGGACGCG AGCGTGTCGGTGTTGCTCGTCTCCCTCAAGGCAGGAGGCGTCGGGCTGAA CCTCACGGCG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig78628:161..820+

>mRNA_M-pyrifera_M_contig78628.18477.1 ID=mRNA_M-pyrifera_M_contig78628.18477.1|Name=mRNA_M-pyrifera_M_contig78628.18477.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=354bp|location=Sequence derived from alignment at M-pyrifera_M_contig78628:161..820+ (Macrocystis pyrifera P11B4 male)
ATGCTCGATTTAGCAGAGACGTCTATGGCTAGGGGGGGCTGGACCTTTAG
GCGTCTGGACGGTGCAATGAGCCAGCAGAGAAGGGAGTCTTCTCTCAAGA
TGCTCGATTTAGCAGAGACGTCTATGGCTAGGGGGGGCTGGACCTTTAGG
CGTCTGGACGGTGCAATGAGCCAGCAGAGAAGGGAGTCTTCTCTCAAGAG
CTTCGACACGGACGCGAGCGTGTCGGTGTTGCTCGTCTCCCTCAAGGCAG
GAGGCGTCGGGCTGAACCTCACGGCGAGCTTCGACACGGACGCGAGCGTG
TCGGTGTTGCTCGTCTCCCTCAAGGCAGGAGGCGTCGGGCTGAACCTCAC
GGCG
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