mRNA_M-pyrifera_M_contig78611.18473.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig78611.18473.1
Unique NamemRNA_M-pyrifera_M_contig78611.18473.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: UPI0018E1DE75 (transmembrane protein 222 n=1 Tax=Cyprinodon tularosa TaxID=77115 RepID=UPI0018E1DE75)

HSP 1 Score: 181 bits (459), Expect = 4.100e-55
Identity = 86/162 (53.09%), Postives = 108/162 (66.67%), Query Frame = 1
Query:   34 KDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
              R PY +VWT IP+L+WL P +GH+GIC S GV  DFAGPY ++ DN+AFGRP +Y  LDV +V A+          A+D AV +AS+EY   MHNLC DNCHSHVA+ALN MRY+ S  WNMVNL +L F  GKY S   F+  W PF ++V +  T+ L
Sbjct:   24 SSRFPYCIVWTPIPVLSWLFPFIGHMGICTSTGVIRDFAGPYFVSEDNMAFGRPTKYWMLDVSKVYASGSN-------AWDTAVRDASEEYKNRMHNLCCDNCHSHVAMALNLMRYENSTSWNMVNLCLLSFIRGKYVSCVGFLKTWLPFLMLVGVVLTVAL 178          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: A0A3B4AVE9_9GOBI (Transmembrane protein 222b n=3 Tax=Gobiidae TaxID=8220 RepID=A0A3B4AVE9_9GOBI)

HSP 1 Score: 180 bits (456), Expect = 1.210e-54
Identity = 85/160 (53.12%), Postives = 109/160 (68.12%), Query Frame = 1
Query:   40 RIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            R PY +VWT IP+L+WL P +GH+GIC S GV  DFAGPY ++ DN+AFGRP +Y  LDV +V A+          A+D AV +AS+EY   MHNLC DNCHSHVA+ALN MRY+ S  WNMVNL +L    GK+ S A F+  W PF ++V +C ++ L
Sbjct:   27 RFPYCIVWTPIPVLSWLLPFIGHMGICTSTGVIRDFAGPYYVSEDNMAFGRPTKYWMLDVSKVYASGSN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVAMALNLMRYENSTSWNMVNLCLLALIHGKHVSCAGFLKTWLPFMMLVLVCLSLTL 179          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: H0XCI4_OTOGA (Uncharacterized protein n=2 Tax=Otolemur garnettii TaxID=30611 RepID=H0XCI4_OTOGA)

HSP 1 Score: 180 bits (457), Expect = 1.800e-54
Identity = 89/170 (52.35%), Postives = 109/170 (64.12%), Query Frame = 1
Query:   10 GEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            G VA D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P +Y +LD  QV A+          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ I  T+ L
Sbjct:   41 GGVAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFVLLLGIILTVSL 203          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: TM222_HUMAN (Transmembrane protein 222 n=14 Tax=Boreoeutheria TaxID=1437010 RepID=TM222_HUMAN)

HSP 1 Score: 180 bits (456), Expect = 2.550e-54
Identity = 89/170 (52.35%), Postives = 109/170 (64.12%), Query Frame = 1
Query:   10 GEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            G VA D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P +Y +LD  QV A+          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ I  T+ L
Sbjct:   41 GGVAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFILLLGIILTVSL 203          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: B4E1F7_HUMAN (cDNA FLJ56305 n=3 Tax=Homininae TaxID=207598 RepID=B4E1F7_HUMAN)

HSP 1 Score: 180 bits (456), Expect = 2.550e-54
Identity = 89/170 (52.35%), Postives = 109/170 (64.12%), Query Frame = 1
Query:   10 GEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            G VA D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P +Y +LD  QV A+          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ I  T+ L
Sbjct:   41 GGVAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPN-------AWDTAVRDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFILLLGIILTVSL 203          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: A0A0D9S830_CHLSB (Transmembrane protein 222 n=33 Tax=Euarchontoglires TaxID=314146 RepID=A0A0D9S830_CHLSB)

HSP 1 Score: 180 bits (456), Expect = 2.630e-54
Identity = 89/170 (52.35%), Postives = 109/170 (64.12%), Query Frame = 1
Query:   10 GEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            G VA D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P +Y +LD  QV A+          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ I  T+ L
Sbjct:   42 GGVAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFILLLGIILTVSL 204          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: U3FHI3_CALJA (Transmembrane protein 222 n=5 Tax=Tetrapoda TaxID=32523 RepID=U3FHI3_CALJA)

HSP 1 Score: 180 bits (456), Expect = 2.710e-54
Identity = 89/170 (52.35%), Postives = 109/170 (64.12%), Query Frame = 1
Query:   10 GEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            G VA D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P +Y +LD  QV A+          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ I  T+ L
Sbjct:   43 GGVAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFILLLGIVLTVSL 205          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: A0A671UMV4_SPAAU (Transmembrane protein 222-like n=9 Tax=Percomorphaceae TaxID=1489872 RepID=A0A671UMV4_SPAAU)

HSP 1 Score: 179 bits (453), Expect = 3.330e-54
Identity = 85/161 (52.80%), Postives = 109/161 (67.70%), Query Frame = 1
Query:   40 RIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILLT 522
            R PY +VWT IP+L+WL P +GH+GIC S G+  DFAGPY ++ DN+AFGRP +Y  LDV +V A+          A+D AV +AS+EY   MHNLC DNCHSHVA+ALN MRY+ S  WNMVNL +L    GK+ S A F+  W PF +++ I  T+ LT
Sbjct:   26 RYPYCIVWTPIPVLSWLLPFIGHMGICTSTGIIRDFAGPYFVSEDNMAFGRPTKYWMLDVSKVYASGSN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVAMALNLMRYENSTSWNMVNLCLLALIHGKHVSCAGFLKTWLPFLMLMGIILTVALT 179          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: A0A1U7TQ73_CARSF (transmembrane protein 222 isoform X2 n=2 Tax=Carlito syrichta TaxID=1868482 RepID=A0A1U7TQ73_CARSF)

HSP 1 Score: 179 bits (455), Expect = 3.610e-54
Identity = 88/170 (51.76%), Postives = 109/170 (64.12%), Query Frame = 1
Query:   10 GEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            G VA D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P++Y +LD  QV  +          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ I  T+ L
Sbjct:   41 GGVAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPVKYWKLDPAQVYTSGPN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFVLLLGIILTVSL 203          
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Match: A0A8J6A7Q6_GALPY (Transmembrane protein 222 n=4 Tax=Laurasiatheria TaxID=314145 RepID=A0A8J6A7Q6_GALPY)

HSP 1 Score: 179 bits (454), Expect = 4.520e-54
Identity = 87/171 (50.88%), Postives = 109/171 (63.74%), Query Frame = 1
Query:    7 EGEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFAGPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASKEYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTSVASFVCHWAPFAVVVAICATILL 519
            +G  A D  + R PY +VWT IP+LTW  P +GH+GIC S GV  DFAGPY ++ DN+AFG+P +Y +LD  QV A+          A+D AV +AS+EY   MHNLC DNCHSHVALALN MRY  S  WNMV L       GKY SV +FV  W PF +++ +  T+ L
Sbjct:   36 QGGAAMDVERSRFPYCVVWTPIPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPN-------AWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVSVGAFVKTWLPFVLLLGVILTVSL 199          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig78611.18473.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI0018E1DE754.100e-5553.09transmembrane protein 222 n=1 Tax=Cyprinodon tular... [more]
A0A3B4AVE9_9GOBI1.210e-5453.13Transmembrane protein 222b n=3 Tax=Gobiidae TaxID=... [more]
H0XCI4_OTOGA1.800e-5452.35Uncharacterized protein n=2 Tax=Otolemur garnettii... [more]
TM222_HUMAN2.550e-5452.35Transmembrane protein 222 n=14 Tax=Boreoeutheria T... [more]
B4E1F7_HUMAN2.550e-5452.35cDNA FLJ56305 n=3 Tax=Homininae TaxID=207598 RepID... [more]
A0A0D9S830_CHLSB2.630e-5452.35Transmembrane protein 222 n=33 Tax=Euarchontoglire... [more]
U3FHI3_CALJA2.710e-5452.35Transmembrane protein 222 n=5 Tax=Tetrapoda TaxID=... [more]
A0A671UMV4_SPAAU3.330e-5452.80Transmembrane protein 222-like n=9 Tax=Percomorpha... [more]
A0A1U7TQ73_CARSF3.610e-5451.76transmembrane protein 222 isoform X2 n=2 Tax=Carli... [more]
A0A8J6A7Q6_GALPY4.520e-5450.88Transmembrane protein 222 n=4 Tax=Laurasiatheria T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig78611contigM-pyrifera_M_contig78611:447..977 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMammalia
Seed ortholog score182.6
Seed ortholog evalue1.6e-43
Seed eggNOG ortholog10141.ENSCPOP00000015928
Preferred nameTMEM222
KEGG koko:K20726
KEGG Pathwayko04016,map04016
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Transmembrane protein 222
EggNOG OGs35KFZ@314146,39E1R@33154,3BBZX@33208,3CT6E@33213,3J7T5@40674,483UH@7711,48UXH@7742,4PWM3@9989,KOG3150@1,KOG3150@2759
COG Functional cat.S
Best tax levelRodentia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Exons1
Model size531
Cds size531
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816688.670542-CDS-M-pyrifera_M_contig78611:446..9771622816688.670542-CDS-M-pyrifera_M_contig78611:446..977Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78611 447..977 -
1692278120.7296884-CDS-M-pyrifera_M_contig78611:446..9771692278120.7296884-CDS-M-pyrifera_M_contig78611:446..977Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78611 447..977 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig78611.18473.1prot_M-pyrifera_M_contig78611.18473.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig78611 447..977 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig78611.18473.1

>prot_M-pyrifera_M_contig78611.18473.1 ID=prot_M-pyrifera_M_contig78611.18473.1|Name=mRNA_M-pyrifera_M_contig78611.18473.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=177bp
LEEGEVAADWSKDRIPYGLVWTAIPMLTWLCPCVGHLGICRSDGVSFDFA
GPYTITVDNLAFGRPLRYVQLDVEQVTAAMDQSRAGKRKAFDAAVENASK
EYTRHMHNLCWDNCHSHVALALNQMRYQGSAGWNMVNLAMLLFFTGKYTS
VASFVCHWAPFAVVVAICATILLTGK*
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mRNA from alignment at M-pyrifera_M_contig78611:447..977-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig78611.18473.1 ID=mRNA_M-pyrifera_M_contig78611.18473.1|Name=mRNA_M-pyrifera_M_contig78611.18473.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=531bp|location=Sequence derived from alignment at M-pyrifera_M_contig78611:447..977- (Macrocystis pyrifera P11B4 male)
TTGGAAGAAGGCGAAGTGGCTGCTGATTGGAGCAAGGACCGGATTCCGTA CGGCTTGGTGTGGACTGCGATTCCGATGCTGACGTGGCTGTGTCCGTGCG TGGGCCACTTGGGCATTTGCCGGAGCGACGGGGTGAGCTTCGATTTTGCA GGGCCCTACACGATCACGGTGGACAATCTGGCGTTCGGACGGCCTCTGCG GTACGTGCAGCTGGACGTGGAGCAGGTCACGGCGGCCATGGACCAGAGCA GGGCCGGGAAACGCAAGGCCTTTGACGCCGCCGTGGAGAATGCCTCCAAG GAGTACACCAGGCACATGCACAACCTCTGCTGGGACAACTGCCACTCCCA CGTCGCCCTCGCGCTCAACCAAATGCGGTACCAGGGCAGCGCGGGGTGGA ACATGGTCAACCTAGCCATGCTGCTCTTTTTCACCGGAAAATACACATCG GTCGCATCCTTCGTGTGCCACTGGGCACCCTTCGCGGTAGTAGTCGCCAT TTGCGCCACGATTCTGCTCACAGGGAAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig78611:447..977-

>mRNA_M-pyrifera_M_contig78611.18473.1 ID=mRNA_M-pyrifera_M_contig78611.18473.1|Name=mRNA_M-pyrifera_M_contig78611.18473.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1062bp|location=Sequence derived from alignment at M-pyrifera_M_contig78611:447..977- (Macrocystis pyrifera P11B4 male)
TTGGAAGAAGGCGAAGTGGCTGCTGATTGGAGCAAGGACCGGATTCCGTA
CGGCTTGGTGTGGACTGCGATTCCGATGCTGACGTGGCTGTGTCCGTGCG
TGGGCCACTTGGGCATTTGCCGGAGCGACGGGGTGAGCTTCGATTTTGCA
GGGCCCTACACGATCACGGTGGACAATCTGGCGTTCGGACGGCCTCTGCG
GTACGTGCAGCTGGACGTGGAGCAGGTCACGGCGGCCATGGACCAGAGCA
GGGCCGGGAAACGCAAGGCCTTTGACGCCGCCGTGGAGAATGCCTCCAAG
GAGTACACCAGGCACATGCACAACCTCTGCTGGGACAACTGCCACTCCCA
CGTCGCCCTCGCGCTCAACCAAATGCGGTACCAGGGCAGCGCGGGGTGGA
ACATGGTCAACCTAGCCATGCTGCTCTTTTTCACCGGAAAATACACATCG
GTCGCATCCTTCGTGTGCCACTGGGCACCCTTCGCGGTAGTAGTCGCCAT
TTGCGCCACGATTCTGCTCACAGGGAAGTGATTGGAAGAAGGCGAAGTGG
CTGCTGATTGGAGCAAGGACCGGATTCCGTACGGCTTGGTGTGGACTGCG
ATTCCGATGCTGACGTGGCTGTGTCCGTGCGTGGGCCACTTGGGCATTTG
CCGGAGCGACGGGGTGAGCTTCGATTTTGCAGGGCCCTACACGATCACGG
TGGACAATCTGGCGTTCGGACGGCCTCTGCGGTACGTGCAGCTGGACGTG
GAGCAGGTCACGGCGGCCATGGACCAGAGCAGGGCCGGGAAACGCAAGGC
CTTTGACGCCGCCGTGGAGAATGCCTCCAAGGAGTACACCAGGCACATGC
ACAACCTCTGCTGGGACAACTGCCACTCCCACGTCGCCCTCGCGCTCAAC
CAAATGCGGTACCAGGGCAGCGCGGGGTGGAACATGGTCAACCTAGCCAT
GCTGCTCTTTTTCACCGGAAAATACACATCGGTCGCATCCTTCGTGTGCC
ACTGGGCACCCTTCGCGGTAGTAGTCGCCATTTGCGCCACGATTCTGCTC
ACAGGGAAGTGA
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