mRNA_M-pyrifera_M_contig76826.18099.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig76826.18099.1
Unique NamemRNA_M-pyrifera_M_contig76826.18099.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A351EQY2_9ACTN (Thiosulfate sulfurtransferase n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A351EQY2_9ACTN)

HSP 1 Score: 173 bits (438), Expect = 1.090e-48
Identity = 80/142 (56.34%), Postives = 102/142 (71.83%), Query Frame = 1
Query:   10 EAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            +   VLD+ SSL +R++GHIPGA W VRSRL +AR A+G  + + +T+ DG LA LA  E  SLWP   V  L  G + W  AG D+E GF  ATT A+D+WYKPYDH+    E+HM++YLTWE+ALV+QLDRDPTV+FPTF
Sbjct:  393 DTTTVLDIGSSLKFRKRGHIPGAWWGVRSRLEQAREAIGDTEYLAITASDGQLAKLAVPEAQSLWPKTTVVALAAGNKGWRHAGYDMEPGFDRATTDANDVWYKPYDHEGDVVEQHMRDYLTWEIALVEQLDRDPTVSFPTF 534          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A520XZE5_9ACTN (Thiosulfate sulfurtransferase n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A520XZE5_9ACTN)

HSP 1 Score: 169 bits (429), Expect = 2.040e-47
Identity = 84/139 (60.43%), Postives = 101/139 (72.66%), Query Frame = 1
Query:   19 VVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            VVLD+ +SL YR KGHIPG+ W VRSRL EA+ A+G    +VLTS DG LA LA AE    WPDA V  L GG +AW  AG D+E GFT+ TTT +D+WYKPY+ DD   + HM +YLTWEVALV+Q++RDPTV FP F
Sbjct:  391 VVLDIGTSLKYRSKGHIPGSWWGVRSRLAEAQEAIGEVATLVLTSTDGQLAKLAVAEAQEHWPDADVVALKGGNKAWRHAGQDMEPGFTNPTTTDNDVWYKPYERDDAVGQ-HMVDYLTWEVALVEQINRDPTVNFPKF 528          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A8J4Z096_9CHEL (Uncharacterized protein n=1 Tax=Nymphon striatum TaxID=424472 RepID=A0A8J4Z096_9CHEL)

HSP 1 Score: 162 bits (411), Expect = 1.250e-44
Identity = 78/139 (56.12%), Postives = 99/139 (71.22%), Query Frame = 1
Query:   22 VLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPD-ARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            VLD+ SSL YR++GHIPG+ W VRSRL +A  A+G AD +V+TS DG LA LA  E   LW    +VS L  G R W  AG D++ GF  ATT A+D+WYKPYDH+    ++HM++YLTWE+ALV+QLDRDPTV F  +
Sbjct:  417 VLDIGSSLKYRKRGHIPGSWWGVRSRLPQAHQAIGEADAVVITSTDGQLAKLAVDEAQELWGRRTQVSALASGNRGWRHAGHDMDTGFDRATTQANDVWYKPYDHEGEVVDQHMRDYLTWEIALVEQLDRDPTVNFQRY 555          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A0S8B1G8_9PROT (Uncharacterized protein n=1 Tax=Betaproteobacteria bacterium SG8_39 TaxID=1703390 RepID=A0A0S8B1G8_9PROT)

HSP 1 Score: 152 bits (385), Expect = 5.340e-41
Identity = 78/142 (54.93%), Postives = 100/142 (70.42%), Query Frame = 1
Query:   13 APVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHD-DGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            A  V+DLA+SL YR + HIPGA W VR+RL  A++ +    R+VLTS DG LA LAA E ++LWP   + VL GG  AW  AG  +E G T ATTT DD+WYKPYDH+ +G+ E+H + YL+WEVALVDQ+ RDPT+ F  +
Sbjct:  394 ATCVVDLATSLRYRAR-HIPGAWWAVRARLAAAQAKLPPPARLVLTSDDGLLARLAAPEAAALWPGLEIRVLEGGNAAWFAAGLPVESGMTRATTTLDDVWYKPYDHEHEGDYEKHARAYLSWEVALVDQIKRDPTIRFRAY 534          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A1F4CUJ7_9PROT (Uncharacterized protein n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_68_19 TaxID=1797504 RepID=A0A1F4CUJ7_9PROT)

HSP 1 Score: 149 bits (376), Expect = 9.410e-40
Identity = 76/144 (52.78%), Postives = 97/144 (67.36%), Query Frame = 1
Query:    4 VDEAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            VD   VV+D ++SL +R + HIPGA W VRSRL EAR  +G   ++VLTS DG LA LAA E ++LWP A+V  L GG  AW  AG   + G   ATT  DD+WYKPYDH   + E+H ++YL+WEVALV+Q+ RDP + F  F
Sbjct:  384 VDAGSVVIDFSTSLRFRAR-HIPGAWWGVRSRLSEAREKIGNPRQLVLTSEDGVLAQLAAPEAAALWPGAQVRFLEGGNAAWFAAGLPAQTGIERATTALDDVWYKPYDHG-ADYEKHARDYLSWEVALVEQIKRDPAIRFRVF 525          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A1F4EZA6_9PROT (Uncharacterized protein n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_65_14 TaxID=1797501 RepID=A0A1F4EZA6_9PROT)

HSP 1 Score: 145 bits (365), Expect = 3.020e-38
Identity = 79/143 (55.24%), Postives = 95/143 (66.43%), Query Frame = 1
Query:    7 DEAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            D +  VLDL+SSL +RR GHIPGA W VRSRL EARSA+G    +VLTS DG LA LAA E   LWP+A V VL GG  AW+    D E GF  ATT  DD+ YKPYDH   + ++H + YL WEVAL +Q+ RDP + F  +
Sbjct:  375 DVSGTVLDLSSSLRFRR-GHIPGAWWGVRSRLGEARSAIGEVKNLVLTSEDGLLAHLAAPEAVGLWPNANVVVLEGGNAAWL---ADRETGFERATTAPDDVAYKPYDHG-SDYQKHARNYLDWEVALAEQIKRDPAIRFRAY 512          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A7C1UIS1_9ACTN (Thiosulfate sulfurtransferase n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7C1UIS1_9ACTN)

HSP 1 Score: 145 bits (365), Expect = 3.950e-38
Identity = 75/148 (50.68%), Postives = 95/148 (64.19%), Query Frame = 1
Query:    7 DEAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSA----VGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEP-ERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            D   VV+D+ SSL +R +GH+P A W VRSRL +   +    +    R+V TS DG+LA+ AA + S+LWP   +  L GGTRAW EAG  LE G     T  DD+WYKPYD   G   E HM+EYL+WEVALVD++ RDP V+FP F
Sbjct:  386 DPNTVVIDIGSSLAHRDRGHVPRAWWTVRSRLGDLVGSGTPEIDGRTRLVFTSSDGSLAAWAADDASALWPGTEIRHLRGGTRAWSEAGRPLETGMNRPLTEVDDVWYKPYDRAQGRAAEEHMREYLSWEVALVDKIARDPLVSFPEF 533          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A8J7MWY0_9PROT (Uncharacterized protein n=1 Tax=Candidatus Odyssella sp. TaxID=2589116 RepID=A0A8J7MWY0_9PROT)

HSP 1 Score: 144 bits (362), Expect = 1.150e-37
Identity = 72/137 (52.55%), Postives = 93/137 (67.88%), Query Frame = 1
Query:   25 LDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTF 435
            +D A+SL YR + H+P A W VR+RL EA++ V     ++LTS DG LA LAA E ++LWP+A ++VL GG  AW  A   +E G   ATTT DD+WYKPYDH     ++HMQ YL WEVALV+Q+ RDPT+ F  F
Sbjct:  405 IDFATSLHYRER-HVPYAWWAVRARLAEAKTFVFNPRALLLTSEDGVLARLAAPEAAALWPNAEIAVLAGGNAAWFAAELPIEKGLDRATTTNDDVWYKPYDHPADALKQHMQSYLDWEVALVEQIKRDPTIRFEAF 540          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A536Y6Y3_9PROT (Thiosulfate sulfurtransferase n=2 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A536Y6Y3_9PROT)

HSP 1 Score: 138 bits (347), Expect = 1.130e-35
Identity = 72/140 (51.43%), Postives = 94/140 (67.14%), Query Frame = 1
Query:    7 DEAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTF 426
            ++   VLD ++SL +  + HIPGA WVVRSRL EAR  +G A+ + LTS DG LA LAA +V++LWP A V VL GG  AW    G  E     A+T  DD+WYKPYDH+    E+H ++YL WEVAL++Q+ RDPT+ F
Sbjct:  374 EDEGTVLDFSTSLRFHHR-HIPGAWWVVRSRLDEARRRIGEAEVLTLTSEDGVLAQLAAPDVAALWPQASVRVLEGGNAAW---PGPWEARVDRASTARDDVWYKPYDHE-ASFEQHARDYLAWEVALIEQIKRDPTIRF 508          
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Match: A0A6L7XFF5_9PROT (Thiosulfate sulfurtransferase n=1 Tax=Rhodospirillales bacterium TaxID=2026786 RepID=A0A6L7XFF5_9PROT)

HSP 1 Score: 138 bits (347), Expect = 1.330e-35
Identity = 73/140 (52.14%), Postives = 94/140 (67.14%), Query Frame = 1
Query:    7 DEAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLTSFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTTADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTF 426
            DEA V+ D +SSL +R  GH+PGA W VRSRL EA + + AA R+VLTS DG LA  AA E + LW  + + VL GG   W++AG   E G   +TT  DDIWY+PY   + + ERH ++YL WEVAL++Q+ RDPTV F
Sbjct:  384 DEAAVI-DFSSSLSFRN-GHVPGAWWGVRSRLEEAYATLPAAKRLVLTSPDGVLAHYAAPEAAELWQSSTIRVLEGGNSGWLDAGLPFETGTDRSTTKLDDIWYRPYQRAE-DYERHARDYLAWEVALLEQIKRDPTVAF 520          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig76826.18099.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A351EQY2_9ACTN1.090e-4856.34Thiosulfate sulfurtransferase n=1 Tax=Acidimicrobi... [more]
A0A520XZE5_9ACTN2.040e-4760.43Thiosulfate sulfurtransferase n=1 Tax=Acidimicrobi... [more]
A0A8J4Z096_9CHEL1.250e-4456.12Uncharacterized protein n=1 Tax=Nymphon striatum T... [more]
A0A0S8B1G8_9PROT5.340e-4154.93Uncharacterized protein n=1 Tax=Betaproteobacteria... [more]
A0A1F4CUJ7_9PROT9.410e-4052.78Uncharacterized protein n=1 Tax=Betaproteobacteria... [more]
A0A1F4EZA6_9PROT3.020e-3855.24Uncharacterized protein n=1 Tax=Betaproteobacteria... [more]
A0A7C1UIS1_9ACTN3.950e-3850.68Thiosulfate sulfurtransferase n=1 Tax=Acidimicrobi... [more]
A0A8J7MWY0_9PROT1.150e-3752.55Uncharacterized protein n=1 Tax=Candidatus Odyssel... [more]
A0A536Y6Y3_9PROT1.130e-3551.43Thiosulfate sulfurtransferase n=2 Tax=Betaproteoba... [more]
A0A6L7XFF5_9PROT1.330e-3552.14Thiosulfate sulfurtransferase n=1 Tax=Rhodospirill... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig76826contigM-pyrifera_M_contig76826:574..1020 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score123.6
Seed ortholog evalue7.6e-26
Seed eggNOG ortholog1123376.AUIU01000017_gene177
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS
EggNOG OGsCOG0607@1,COG0607@2
COG Functional cat.P
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size447
Cds size447
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816667.532887-CDS-M-pyrifera_M_contig76826:573..10201622816667.532887-CDS-M-pyrifera_M_contig76826:573..1020Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig76826 574..1020 -
1692278108.087606-CDS-M-pyrifera_M_contig76826:573..10201692278108.087606-CDS-M-pyrifera_M_contig76826:573..1020Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig76826 574..1020 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig76826.18099.1prot_M-pyrifera_M_contig76826.18099.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig76826 574..1020 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig76826.18099.1

>prot_M-pyrifera_M_contig76826.18099.1 ID=prot_M-pyrifera_M_contig76826.18099.1|Name=mRNA_M-pyrifera_M_contig76826.18099.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=149bp
EVDEAPVVLDLASSLDYRRKGHIPGAHWVVRSRLREARSAVGAADRIVLT
SFDGALASLAAAEVSSLWPDARVSVLTGGTRAWIEAGGDLEVGFTSATTT
ADDIWYKPYDHDDGEPERHMQEYLTWEVALVDQLDRDPTVTFPTFPTF*
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mRNA from alignment at M-pyrifera_M_contig76826:574..1020-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig76826.18099.1 ID=mRNA_M-pyrifera_M_contig76826.18099.1|Name=mRNA_M-pyrifera_M_contig76826.18099.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=447bp|location=Sequence derived from alignment at M-pyrifera_M_contig76826:574..1020- (Macrocystis pyrifera P11B4 male)
GAAGTCGACGAGGCGCCGGTCGTGCTCGACCTTGCGTCGAGTTTGGACTA CCGCCGCAAAGGCCACATCCCGGGTGCTCACTGGGTCGTGCGGAGTCGTT TGCGTGAAGCTCGGTCGGCCGTCGGTGCGGCAGATCGGATCGTCCTGACG TCATTCGACGGCGCGCTGGCCTCACTCGCGGCGGCCGAGGTGTCGAGCCT GTGGCCCGACGCTCGGGTGTCGGTGCTGACCGGCGGAACCCGAGCATGGA TCGAGGCAGGTGGAGATCTCGAGGTCGGTTTCACCTCAGCCACGACGACC GCCGACGACATCTGGTACAAGCCGTACGATCACGACGACGGCGAGCCCGA GCGTCACATGCAGGAGTACCTGACATGGGAGGTCGCGCTGGTCGACCAAC TCGATCGTGACCCGACGGTGACCTTCCCCACCTTCCCCACCTTCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig76826:574..1020-

>mRNA_M-pyrifera_M_contig76826.18099.1 ID=mRNA_M-pyrifera_M_contig76826.18099.1|Name=mRNA_M-pyrifera_M_contig76826.18099.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=894bp|location=Sequence derived from alignment at M-pyrifera_M_contig76826:574..1020- (Macrocystis pyrifera P11B4 male)
GAAGTCGACGAGGCGCCGGTCGTGCTCGACCTTGCGTCGAGTTTGGACTA
CCGCCGCAAAGGCCACATCCCGGGTGCTCACTGGGTCGTGCGGAGTCGTT
TGCGTGAAGCTCGGTCGGCCGTCGGTGCGGCAGATCGGATCGTCCTGACG
TCATTCGACGGCGCGCTGGCCTCACTCGCGGCGGCCGAGGTGTCGAGCCT
GTGGCCCGACGCTCGGGTGTCGGTGCTGACCGGCGGAACCCGAGCATGGA
TCGAGGCAGGTGGAGATCTCGAGGTCGGTTTCACCTCAGCCACGACGACC
GCCGACGACATCTGGTACAAGCCGTACGATCACGACGACGGCGAGCCCGA
GCGTCACATGCAGGAGTACCTGACATGGGAGGTCGCGCTGGTCGACCAAC
TCGATCGTGACCCGACGGTGACCTTCCCCACCTTCCCCACCTTCTGAGAA
GTCGACGAGGCGCCGGTCGTGCTCGACCTTGCGTCGAGTTTGGACTACCG
CCGCAAAGGCCACATCCCGGGTGCTCACTGGGTCGTGCGGAGTCGTTTGC
GTGAAGCTCGGTCGGCCGTCGGTGCGGCAGATCGGATCGTCCTGACGTCA
TTCGACGGCGCGCTGGCCTCACTCGCGGCGGCCGAGGTGTCGAGCCTGTG
GCCCGACGCTCGGGTGTCGGTGCTGACCGGCGGAACCCGAGCATGGATCG
AGGCAGGTGGAGATCTCGAGGTCGGTTTCACCTCAGCCACGACGACCGCC
GACGACATCTGGTACAAGCCGTACGATCACGACGACGGCGAGCCCGAGCG
TCACATGCAGGAGTACCTGACATGGGAGGTCGCGCTGGTCGACCAACTCG
ATCGTGACCCGACGGTGACCTTCCCCACCTTCCCCACCTTCTGA
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