mRNA_M-pyrifera_M_contig75110.17745.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig75110.17745.1
Unique NamemRNA_M-pyrifera_M_contig75110.17745.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: UPI001E68D7A0 (ATP-dependent DNA helicase PIF1-like n=1 Tax=Maniola jurtina TaxID=191418 RepID=UPI001E68D7A0)

HSP 1 Score: 111 bits (277), Expect = 8.330e-26
Identity = 66/164 (40.24%), Postives = 97/164 (59.15%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEV--ISEEYPIVKIPRVCFQVQVGTRG-ITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFI 531
            V+ LN +Q    P  DL +K+  + + IRN++F   L+NG K I+   S R++ V    +S++Y    IPR+ F+  V     I + R QFP+++CYAMTI K+QG+T+  VG+D+R  VF  GQLYV   R   R N+  L+  ER+I G P  +NVVY   +
Sbjct:  227 VDALNRMQPKGIPEHDLTLKVGCVCMIIRNLNFSDKLVNGTKVIVVNTSPRLITVRKPGLSDDY---LIPRIIFKAPVEPGSPIEMCRRQFPLQVCYAMTIHKSQGQTICRVGVDLRSDVFSHGQLYVALSRAQHRQNIKILISEERVINGNPLANNVVYPPLL 387          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A6P5FUE2_ANACO (ATP-dependent DNA helicase n=1 Tax=Ananas comosus TaxID=4615 RepID=A0A6P5FUE2_ANACO)

HSP 1 Score: 111 bits (278), Expect = 3.510e-25
Identity = 69/162 (42.59%), Postives = 98/162 (60.49%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVISEEYPIVK--IPRVCFQVQVGTRGITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKP-ERLIKGVPHVHNVVYSE 525
            V+ LNS++    P  +LH+KI A  + +RNI+  +GL NG + II  ++ RV+  ++ +  +   K  IPR+   V       TL+R QFP+RLCYAMTI+K+QG+TL+ VGL +   VF  GQLYV   R T R  L  LV+  E  +KG  +  N+VYSE
Sbjct: 1089 VDFLNSLKFNGVPHHELHLKIGAPIMLLRNINQSAGLCNGTRLIITQLASRVIEAQIFTGNHAGEKVYIPRIIMHVTETKWPFTLKRRQFPIRLCYAMTINKSQGQTLEKVGLYLPRSVFSHGQLYVGVSRVTSRKGLRILVETKEESLKG--YTQNIVYSE 1248          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: UPI001E688A66 (uncharacterized protein LOC123868181 n=2 Tax=Maniola jurtina TaxID=191418 RepID=UPI001E688A66)

HSP 1 Score: 111 bits (277), Expect = 4.780e-25
Identity = 67/164 (40.85%), Postives = 97/164 (59.15%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEV--ISEEYPIVKIPRVCFQVQVGTRG-ITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFI 531
            V+ LN +Q    P  DL +K+  + + IRN++F   L+NG K I+   S R++ V    +S++Y    IPR+ F+  V     I + R QFP+++CYAMTI K+QG+T+  VG+D+R  VF  GQLYV   R   R N+  LV  ER+I G P  +NVVY   +
Sbjct: 1083 VDALNRMQPKGIPEHDLTLKVGCVCMVIRNLNFSDKLVNGTKVIVVNTSPRLITVRKPGLSDDY---LIPRIIFKAPVEPGSPIEMCRRQFPLQVCYAMTIHKSQGQTICRVGVDLRSDVFSHGQLYVALSRAQHRQNIKILVNEERVINGNPLANNVVYPPLL 1243          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A6J1TE18_FRAOC (ATP-dependent DNA helicase n=1 Tax=Frankliniella occidentalis TaxID=133901 RepID=A0A6J1TE18_FRAOC)

HSP 1 Score: 104 bits (259), Expect = 8.660e-25
Identity = 60/163 (36.81%), Postives = 96/163 (58.90%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVISEEYPIVKIPRVCFQVQVGTRGITLR--RFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFI 531
            ++ L+  +    P   L +KI A+ + +RN++    L+NG K I+ GIS R++ V     +  ++ IPR+ F+  V   G  L+  R QFP+++CY MT+ K+QG+T+  VGLD+R   F  GQLYV   RT R D++  L  P+R +  + +V N+VY E +
Sbjct:   28 IDTLHLAEGKGVPNHILDLKIGAVCMIMRNLNMSERLVNGTKVIVEGISPRLVTVRKPGTQ-ELIAIPRILFRFPV-LEGSPLQMCRRQFPLQVCYGMTVHKSQGQTIFKVGLDLRSDCFTHGQLYVALSRTRRSDDVTVLGAPDRCVDNITYVRNIVYQELL 188          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: UPI001568188F (uncharacterized protein LOC117996074 n=2 Tax=Aphantopus hyperantus TaxID=2795564 RepID=UPI001568188F)

HSP 1 Score: 110 bits (275), Expect = 9.150e-25
Identity = 65/164 (39.63%), Postives = 97/164 (59.15%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEV--ISEEYPIVKIPRVCFQVQVGTRG-ITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFI 531
            V+ LN +     P  DL +K+  + + +RN++F   L+NG K I+   S R++ V    +S++Y    IPR+ F+  + T   I + R QFP+++CYAMTI K+QG+T+  VG+D+R  VF  GQLYV   R   R N+  LV  ER+I G P  +NVVY   +
Sbjct: 1642 VDALNRMHPKGIPEHDLTLKVGCVCMIMRNLNFSDKLVNGTKVIVVNTSPRLITVRKPGLSDDY---LIPRIIFKAPIETGSPIEMCRRQFPLQVCYAMTIHKSQGQTICRVGVDLRSDVFSHGQLYVALSRVQHRQNIKILVNKERVINGNPLANNVVYPPLL 1802          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A317YKU2_MAIZE (ATP-dependent DNA helicase (Fragment) n=1 Tax=Zea mays TaxID=4577 RepID=A0A317YKU2_MAIZE)

HSP 1 Score: 108 bits (269), Expect = 5.010e-24
Identity = 72/187 (38.50%), Postives = 103/187 (55.08%), Query Frame = 1
Query:    4 DKIIAGYENIPDVGSV---EHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVIS-----EEYPIVKIPRVCFQVQVGTRGITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFIVAA 540
            D I    ENIPD   +   E LNS+     P   L +K  A+ + +RN++ + GL NG + ++  +  R++H   ++     EE   V IPR+           TL+R QFPVRLCYAMTI+K+QG+TL  VG+ ++  VF  GQLYV   R+T R+ L+ L++ E    G     NVVY E + AA
Sbjct:  475 DVIAKSSENIPDFDVLYPTEFLNSIDANNFPTHKLELKKGAIVMLLRNLNQNKGLCNGTRLLVTQLGQRILHCVALTGSNVGEE---VLIPRIALNTTDVKWPFTLQRRQFPVRLCYAMTINKSQGQTLSRVGIYLKRPVFTHGQLYVAVSRSTSRNGLIILIENEDGSCG-SQTRNVVYKEVLAAA 657          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A3L6EV34_MAIZE (ATP-dependent DNA helicase n=1 Tax=Zea mays TaxID=4577 RepID=A0A3L6EV34_MAIZE)

HSP 1 Score: 105 bits (263), Expect = 2.160e-23
Identity = 70/186 (37.63%), Postives = 102/186 (54.84%), Query Frame = 1
Query:    4 DKIIAGYENIPDVGSV---EHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVIS-----EEYPIVKIPRVCFQVQVGTRGITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFIVA 537
            D I    E+IPD   +   E LNS+     P   L +K  A+ + +RN++ + GL NG + ++  +  R++H   ++     EE   V IPR+           TL+R QFPVRLCYAMTI+K+ G+TL  VG+ ++G VF  GQLYV   R+T R+ L+ L++ E    G     NVVY E + A
Sbjct:  314 DVIAKSSEHIPDFDVLYPTEFLNSIDANNFPTHKLELKKGAIVMLLRNLNQNKGLYNGTRLLVTQLGQRILHCVALTGSNVGEE---VLIPRIALNTTDVKWPFTLQRRQFPVRLCYAMTINKSHGQTLSRVGIYLKGPVFTHGQLYVAVSRSTSRNGLIILIENEDGSCG-SQTRNVVYKEVLAA 495          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A6P5EEX1_ANACO (ATP-dependent DNA helicase n=1 Tax=Ananas comosus TaxID=4615 RepID=A0A6P5EEX1_ANACO)

HSP 1 Score: 105 bits (262), Expect = 4.710e-23
Identity = 65/162 (40.12%), Postives = 98/162 (60.49%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVISEEY--PIVKIPRVCFQVQVGTRGITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKP-ERLIKGVPHVHNVVYSE 525
            V+ LNS++    P  +L +KI A  + +RNI+  +GL NG + +I  ++ RV+  ++ +  +    V IPR+   V       TL+R QFPVRLCYAMTI+K+Q +TL+ VGL +   VF  GQLYV   R T RD +  L++  E+ ++G  +  N+VYSE
Sbjct:  732 VDFLNSLKFNGVPNHELKLKIGAPIMLLRNINQSAGLCNGTRLVITQLASRVIEAQIFTGNHVGEKVYIPRIVMNVTETKWPFTLKRRQFPVRLCYAMTINKSQEQTLQKVGLYLPRPVFSHGQLYVGVSRVTSRDGIRILIEDKEKTLEG--YTQNIVYSE 891          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A397ZA99_BRACM (ATP-dependent DNA helicase (Fragment) n=1 Tax=Brassica campestris TaxID=3711 RepID=A0A397ZA99_BRACM)

HSP 1 Score: 102 bits (254), Expect = 9.420e-23
Identity = 62/161 (38.51%), Postives = 92/161 (57.14%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVISEEY--PIVKIPRVCFQVQVGTRGITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSE 525
            VE+LNS+  P  P   L +K+ A F+ +RN++   GL NG + I+  +  RV+  E+++  +    V +PR+     V     TL R QFP+RLCYAMTI+K+QG++LK V L +   VF  GQLYV   R T +  L  +   +R  + +  V N+VY E
Sbjct:  148 VEYLNSLSFPGLPAHKLTLKVGAPFMLLRNLNQKKGLCNGTRLIVTYLGERVIRGEIVTGSHIGHKVDLPRIILSESVTKHPFTLERRQFPIRLCYAMTINKSQGQSLKGVVLYLPQPVFTHGQLYVGLSRVTTKSGLKII---QRKDQKIGRVKNIVYKE 305          
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Match: A0A1Q5UDF3_9EURO (ATP-dependent DNA helicase n=1 Tax=Penicillium subrubescens TaxID=1316194 RepID=A0A1Q5UDF3_9EURO)

HSP 1 Score: 104 bits (259), Expect = 1.190e-22
Identity = 63/163 (38.65%), Postives = 96/163 (58.90%), Query Frame = 1
Query:   49 VEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFDSGLINGKKGIIRGISLRVMHVEVISEEYPIVK--IPRVCFQVQVGTRGITLRRFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRDNLLCLVKPERLIKGVPHVHNVVYSEFI 531
            +E+L+S+ +P  PP  L +K+ A  + IRN+  + GL NG + II G+    +   +I+ ++  V+  IPR+  +   G    TLRR QFP+RLC+AMTI+K+QG++LK VG+D+R   F  GQLYV   R T    L  L +     +G+    NVV+ E +
Sbjct:  723 LEYLHSINMPGLPPSMLALKVGAPIMLIRNLRQEDGLCNGTRLIITGLYRWNITARIIAGDHKRVEHTIPRIPLETTEGQLSFTLRRVQFPIRLCFAMTINKSQGQSLKQVGVDLRVPAFSHGQLYVALSRVTDVAKLSVLHE-----EGLDTTENVVFPEVL 880          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig75110.17745.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001E68D7A08.330e-2640.24ATP-dependent DNA helicase PIF1-like n=1 Tax=Manio... [more]
A0A6P5FUE2_ANACO3.510e-2542.59ATP-dependent DNA helicase n=1 Tax=Ananas comosus ... [more]
UPI001E688A664.780e-2540.85uncharacterized protein LOC123868181 n=2 Tax=Manio... [more]
A0A6J1TE18_FRAOC8.660e-2536.81ATP-dependent DNA helicase n=1 Tax=Frankliniella o... [more]
UPI001568188F9.150e-2539.63uncharacterized protein LOC117996074 n=2 Tax=Aphan... [more]
A0A317YKU2_MAIZE5.010e-2438.50ATP-dependent DNA helicase (Fragment) n=1 Tax=Zea ... [more]
A0A3L6EV34_MAIZE2.160e-2337.63ATP-dependent DNA helicase n=1 Tax=Zea mays TaxID=... [more]
A0A6P5EEX1_ANACO4.710e-2340.12ATP-dependent DNA helicase n=1 Tax=Ananas comosus ... [more]
A0A397ZA99_BRACM9.420e-2338.51ATP-dependent DNA helicase (Fragment) n=1 Tax=Bras... [more]
A0A1Q5UDF3_9EURO1.190e-2238.65ATP-dependent DNA helicase n=1 Tax=Penicillium sub... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig75110contigM-pyrifera_M_contig75110:468..1010 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score104.8
Seed ortholog evalue4.4e-20
Seed eggNOG ortholog90675.XP_010436751.1
KEGG koko:K07466,ko:K15255
KEGG Pathwayko03030,ko03420,ko03430,ko03440,ko03460,map03030,map03420,map03430,map03440,map03460
KEGG ModuleM00288
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ATP-dependent DNA helicase
EggNOG OGs37Q72@33090,3GCCF@35493,3HWI1@3699,COG0507@1,KOG0987@2759
EC3.6.4.12
COG Functional cat.D
Best tax levelBrassicales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03000,ko03029,ko03032,ko03400
Exons1
Model size543
Cds size543
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816649.5767937-CDS-M-pyrifera_M_contig75110:467..10101622816649.5767937-CDS-M-pyrifera_M_contig75110:467..1010Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig75110 468..1010 +
1692278098.443831-CDS-M-pyrifera_M_contig75110:467..10101692278098.443831-CDS-M-pyrifera_M_contig75110:467..1010Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig75110 468..1010 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig75110.17745.1prot_M-pyrifera_M_contig75110.17745.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig75110 468..1010 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig75110.17745.1

>prot_M-pyrifera_M_contig75110.17745.1 ID=prot_M-pyrifera_M_contig75110.17745.1|Name=mRNA_M-pyrifera_M_contig75110.17745.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=181bp
ADKIIAGYENIPDVGSVEHLNSVQVPATPPRDLHVKIRALFVFIRNIDFD
SGLINGKKGIIRGISLRVMHVEVISEEYPIVKIPRVCFQVQVGTRGITLR
RFQFPVRLCYAMTISKAQGRTLKIVGLDVRGGVFFPGQLYVDFRRTTRRD
NLLCLVKPERLIKGVPHVHNVVYSEFIVAAT
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mRNA from alignment at M-pyrifera_M_contig75110:468..1010+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig75110.17745.1 ID=mRNA_M-pyrifera_M_contig75110.17745.1|Name=mRNA_M-pyrifera_M_contig75110.17745.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=543bp|location=Sequence derived from alignment at M-pyrifera_M_contig75110:468..1010+ (Macrocystis pyrifera P11B4 male)
GCAGACAAAATCATAGCCGGCTACGAAAACATACCAGACGTCGGTTCGGT CGAGCACTTGAACAGTGTGCAAGTGCCTGCCACTCCTCCTCGTGACCTAC ACGTCAAGATTAGAGCTTTGTTTGTTTTTATTCGCAACATAGATTTCGAC AGTGGTCTCATCAACGGAAAGAAAGGAATAATCAGAGGTATTTCACTGCG GGTTATGCACGTCGAGGTCATATCGGAGGAATATCCTATCGTCAAAATTC CACGCGTATGTTTCCAAGTTCAAGTTGGGACCCGTGGTATTACTTTACGC CGATTTCAATTCCCTGTGCGTTTATGCTACGCTATGACCATTAGTAAAGC ACAAGGGCGGACTTTGAAAATAGTAGGGCTCGACGTCAGAGGCGGAGTGT TTTTCCCTGGGCAGCTGTATGTCGATTTTCGTAGAACCACCCGTCGGGAC AACCTTCTATGCCTCGTTAAGCCCGAACGGCTTATCAAGGGAGTGCCTCA CGTTCACAACGTCGTCTATTCCGAGTTTATCGTTGCCGCTACA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig75110:468..1010+

>mRNA_M-pyrifera_M_contig75110.17745.1 ID=mRNA_M-pyrifera_M_contig75110.17745.1|Name=mRNA_M-pyrifera_M_contig75110.17745.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1086bp|location=Sequence derived from alignment at M-pyrifera_M_contig75110:468..1010+ (Macrocystis pyrifera P11B4 male)
GCAGACAAAATCATAGCCGGCTACGAAAACATACCAGACGTCGGTTCGGT
CGAGCACTTGAACAGTGTGCAAGTGCCTGCCACTCCTCCTCGTGACCTAC
ACGTCAAGATTAGAGCTTTGTTTGTTTTTATTCGCAACATAGATTTCGAC
AGTGGTCTCATCAACGGAAAGAAAGGAATAATCAGAGGTATTTCACTGCG
GGTTATGCACGTCGAGGTCATATCGGAGGAATATCCTATCGTCAAAATTC
CACGCGTATGTTTCCAAGTTCAAGTTGGGACCCGTGGTATTACTTTACGC
CGATTTCAATTCCCTGTGCGTTTATGCTACGCTATGACCATTAGTAAAGC
ACAAGGGCGGACTTTGAAAATAGTAGGGCTCGACGTCAGAGGCGGAGTGT
TTTTCCCTGGGCAGCTGTATGTCGATTTTCGTAGAACCACCCGTCGGGAC
AACCTTCTATGCCTCGTTAAGCCCGAACGGCTTATCAAGGGAGTGCCTCA
CGTTCACAACGTCGTCTATTCCGAGTTTATCGTTGCCGCTACAGCAGACA
AAATCATAGCCGGCTACGAAAACATACCAGACGTCGGTTCGGTCGAGCAC
TTGAACAGTGTGCAAGTGCCTGCCACTCCTCCTCGTGACCTACACGTCAA
GATTAGAGCTTTGTTTGTTTTTATTCGCAACATAGATTTCGACAGTGGTC
TCATCAACGGAAAGAAAGGAATAATCAGAGGTATTTCACTGCGGGTTATG
CACGTCGAGGTCATATCGGAGGAATATCCTATCGTCAAAATTCCACGCGT
ATGTTTCCAAGTTCAAGTTGGGACCCGTGGTATTACTTTACGCCGATTTC
AATTCCCTGTGCGTTTATGCTACGCTATGACCATTAGTAAAGCACAAGGG
CGGACTTTGAAAATAGTAGGGCTCGACGTCAGAGGCGGAGTGTTTTTCCC
TGGGCAGCTGTATGTCGATTTTCGTAGAACCACCCGTCGGGACAACCTTC
TATGCCTCGTTAAGCCCGAACGGCTTATCAAGGGAGTGCCTCACGTTCAC
AACGTCGTCTATTCCGAGTTTATCGTTGCCGCTACA
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