mRNA_M-pyrifera_M_contig74613.17655.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig74613.17655.1
Unique NamemRNA_M-pyrifera_M_contig74613.17655.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: A0A2D9JCP9_9BACT (DUF1990 domain-containing protein n=2 Tax=Rhodopirellula TaxID=265488 RepID=A0A2D9JCP9_9BACT)

HSP 1 Score: 294 bits (753), Expect = 4.880e-99
Identity = 140/189 (74.07%), Postives = 163/189 (86.24%), Query Frame = 1
Query:    1 EAPLFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAMR 567
            E PLFSLTKPTH+Q+L+ I+EQS+SP NYPDVGA+   FP GYEHH HR+ LG G  VF+ A VALENW+QFDVGWAEAIP +T I VG+TIAIRVRIFG+WAVAFDRIVDV SE +G+  RFG SVGTLM+HPEQGEERF IEID +GQVDYEVAAFFRPNT+AAK+AWP L RRFN+FR+QSA+A++
Sbjct:    8 EVPLFSLTKPTHQQLLQRITEQSRSPFNYPDVGASLGEFPAGYEHHHHRVRLGSGQEVFDKAMVALENWRQFDVGWAEAIPTDTSITVGNTIAIRVRIFGVWAVAFDRIVDVFSEQEGECHRFGFSVGTLMEHPEQGEERFLIEIDEEGQVDYEVAAFFRPNTLAAKIAWPVLHRRFNRFRNQSAEALK 196          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: A0A2G1W2S8_9BACT (DUF1990 domain-containing protein n=1 Tax=Rhodopirellula bahusiensis TaxID=2014065 RepID=A0A2G1W2S8_9BACT)

HSP 1 Score: 291 bits (744), Expect = 3.430e-98
Identity = 138/174 (79.31%), Postives = 154/174 (88.51%), Query Frame = 1
Query:   76 PLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAMRRQVDSVDLSQ 597
            P NYPDVGA+   FP+GYEHHRHR+ LG G   F  AK ALE+W+QFDVGWA+AIP +T I +GST+AIRVRIFG+WA+AFDRIVDVHSESD D+QRFG SVGTLMDHPEQGEE+FSIEID DGQVDYEVAAFFRPNT AAK+AWPYL RRFNQFRDQSA+AMRRQVDSV+LSQ
Sbjct:    2 PFNYPDVGASTGEFPEGYEHHRHRIRLGRGHDTFNRAKDALEHWRQFDVGWAKAIPSDTSITIGSTVAIRVRIFGIWALAFDRIVDVHSESDRDQQRFGFSVGTLMDHPEQGEEQFSIEIDGDGQVDYEVAAFFRPNTFAAKIAWPYLHRRFNQFRDQSAEAMRRQVDSVELSQ 175          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: Q7UV48_RHOBA (DUF1990 domain-containing protein n=5 Tax=Rhodopirellula baltica TaxID=265606 RepID=Q7UV48_RHOBA)

HSP 1 Score: 286 bits (732), Expect = 1.040e-95
Identity = 135/188 (71.81%), Postives = 159/188 (84.57%), Query Frame = 1
Query:    1 EAPLFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAM 564
            E PLFSLTKP+H+++L+ I+EQS+SP NYPDVGA+   FP GYEHH HR+ LG G  VF+ AK AL NW+QFDVGW EAIP +T I VG+TIAIRVRIFG+WAVAFDRIVDV+ E DG+ +RFG SVGTL +HPEQGEERFSIEID +  VDYEVAAFFRPNT+AAK+AWP L RRFN+FR+QSA+AM
Sbjct:   22 EVPLFSLTKPSHQELLQKIAEQSRSPFNYPDVGASLCEFPSGYEHHHHRVRLGRGQEVFDRAKEALGNWRQFDVGWVEAIPSDTSITVGNTIAIRVRIFGVWAVAFDRIVDVYGEQDGECRRFGFSVGTLTEHPEQGEERFSIEIDREDHVDYEVAAFFRPNTLAAKIAWPVLHRRFNRFRNQSAEAM 209          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: M2B5D1_9BACT (Uncharacterized conserved protein UCP010260 n=1 Tax=Rhodopirellula europaea 6C TaxID=1263867 RepID=M2B5D1_9BACT)

HSP 1 Score: 276 bits (705), Expect = 7.020e-92
Identity = 131/185 (70.81%), Postives = 155/185 (83.78%), Query Frame = 1
Query:   10 LFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAM 564
            +F+L KPTH+Q+L  IS+QS+S  NYPDVGA++  FPDGYEHHRHR+ LG+G  +FESAK ALENW+QFDVGW EA P  T I VG+TIAIRVRIFG+WAVAFDRIVDV  E   +  RFG SVGTLM+HPEQGEERF IE+D D +VDYEVAAFFRPNT+AAK+AWP L RRFN+FR+QSA+A+
Sbjct:    1 MFTLAKPTHQQLLHRISQQSRSSFNYPDVGASQRAFPDGYEHHRHRVSLGNGREIFESAKAALENWRQFDVGWVEAFPANTAITVGNTIAIRVRIFGVWAVAFDRIVDVFDEQKENCHRFGYSVGTLMEHPEQGEERFLIEMDDDCKVDYEVAAFFRPNTLAAKIAWPILHRRFNRFRNQSAEAL 185          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: UPI001E3C47E2 (DUF1990 domain-containing protein n=1 Tax=Rhodopirellula baltica TaxID=265606 RepID=UPI001E3C47E2)

HSP 1 Score: 237 bits (605), Expect = 3.790e-77
Identity = 113/157 (71.97%), Postives = 131/157 (83.44%), Query Frame = 1
Query:   94 VGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAM 564
            +GA+   FP GYEHH HR+ LG G  VF+ AK AL NW+QFDVGW EAIP +T I VG+TIAIRVRIFG+WAVAFDRIVDV+ E DG+ +RFG SVGTL +HPEQGEERFSIEID +  VDYEVAAFFRPNT+AAK+AWP L RRFN+FR+QSA+AM
Sbjct:    1 MGASLCEFPSGYEHHHHRVRLGRGQEVFDRAKEALGNWRQFDVGWVEAIPSDTSITVGNTIAIRVRIFGVWAVAFDRIVDVYGEQDGECRRFGFSVGTLTEHPEQGEERFSIEIDREDHVDYEVAAFFRPNTLAAKIAWPVLHRRFNRFRNQSAEAM 157          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: M5S1K9_9BACT (DUF1990 domain-containing protein n=1 Tax=Rhodopirellula europaea SH398 TaxID=1263868 RepID=M5S1K9_9BACT)

HSP 1 Score: 215 bits (548), Expect = 1.070e-68
Identity = 103/137 (75.18%), Postives = 120/137 (87.59%), Query Frame = 1
Query:  154 LGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAM 564
            +G G  VF+ A VALENW+QFDVGWAEAIP +T I VG+TIAIRVRIFG+WAVAFDRIVD  SE +G+ +RFG SVGTLM+HPEQGEERF IEID +GQVDYEVAAFFRPNT+AAK+AWP L RRFN+FR+QSA+A+
Sbjct:    1 MGSGQEVFDKAMVALENWRQFDVGWAEAIPTDTSITVGNTIAIRVRIFGVWAVAFDRIVDAFSEQEGECRRFGFSVGTLMEHPEQGEERFLIEIDEEGQVDYEVAAFFRPNTLAAKIAWPVLHRRFNRFRNQSAEAL 137          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: A0A5C6BDJ1_9BACT (DUF1990 domain-containing protein n=1 Tax=Stieleria varia TaxID=2528005 RepID=A0A5C6BDJ1_9BACT)

HSP 1 Score: 206 bits (523), Expect = 2.660e-64
Identity = 99/189 (52.38%), Postives = 128/189 (67.72%), Query Frame = 1
Query:   10 LFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAMRRQV 576
            +F+LT+P  + I   +++QS+  LNYPD  A+    P  Y+H+   + LG+G  +F +A  A  +W+QFDVGW EA+P+ETPI +G  IAIR R+F LW +A  RIVDV  E D D +RF  S+GTL  HPEQGEERF I     G+V Y V AFFRPN+ AA +AWPYLR RFN FR QSA  +RR+V
Sbjct:    1 MFTLTQPNDKTIAAFLAQQSRQDLNYPDTFASLGNLPASYDHNVAEVHLGNGEAIFAAALTAFRHWRQFDVGWVEALPRETPIEIGQNIAIRARVFRLWTLAACRIVDVMDEYDSDTRRFAFSIGTLPAHPEQGEERFEINQSSCGRVTYRVTAFFRPNSTAATVAWPYLRYRFNLFRRQSAGVIRREV 189          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: A0A5M6CVC5_9BACT (DUF1990 domain-containing protein n=1 Tax=Roseiconus nitratireducens TaxID=2605748 RepID=A0A5M6CVC5_9BACT)

HSP 1 Score: 197 bits (501), Expect = 1.450e-60
Identity = 95/185 (51.35%), Postives = 126/185 (68.11%), Query Frame = 1
Query:   10 LFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAM 564
            LFSL +P    +   + EQ+Q   NYPDVGA+R +FP  Y+H    +VLG G  VF++A++A+ENWQ F+VGWAEAIP  TPI     +AIR R+ G+W +A  R+++V  +   D +RFG S GTL  HPEQGEERF I +  DG+V Y VAAFFR N  A  +AWPYLR+RFN+FR ++A  +
Sbjct:   28 LFSLFRPDDAAVRVFLREQAQLEFNYPDVGASRGQFPLRYDHDHFEVVLGRGRDVFDAARIAIENWQHFNVGWAEAIPLSTPITAADNVAIRARLGGIWGLAAGRVIEVIDDLSPDGERFGFSFGTLPGHPEQGEERFEICLMPDGRVRYRVAAFFRSNHWAIGIAWPYLRQRFNRFRRETAKVL 212          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: A0A517MD82_9BACT (DUF1990 domain-containing protein n=1 Tax=Roseimaritima multifibrata TaxID=1930274 RepID=A0A517MD82_9BACT)

HSP 1 Score: 185 bits (469), Expect = 3.990e-56
Identity = 92/189 (48.68%), Postives = 118/189 (62.43%), Query Frame = 1
Query:   10 LFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRLVLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAMRRQV 576
            + S TKP    I R + +Q++   NYPDVGAT    P  Y+H+     +G  +  F+ AK A+  W+ F+VGWA A P  TPIR G  +AI  RI GLWA+A  RI++V  E     +RFG S GTL  HPEQGEERF +    +G V Y + AFFRPN ++A++AWPY R RFNQFR QS D +RR V
Sbjct:    1 MLSFTKPNADSIARFLDQQAKLDFNYPDVGATDGELPSHYDHNSIEEEVGRRATDFQEAKAAMLAWKHFNVGWAAACPTTTPIREGENVAILARISGLWALAACRIIEVLHEESPTSKRFGYSFGTLPGHPEQGEERFEVRCSANGIVTYRITAFFRPNCLSAEIAWPYFRYRFNQFRRQSVDRLRRHV 189          
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Match: F2B257_RHOBT (DUF1990 domain-containing protein n=2 Tax=Rhodopirellula baltica TaxID=265606 RepID=F2B257_RHOBT)

HSP 1 Score: 174 bits (442), Expect = 5.200e-53
Identity = 85/111 (76.58%), Postives = 98/111 (88.29%), Query Frame = 1
Query:  232 EAIPKETPIRVGSTIAIRVRIFGLWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQVDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAM 564
            EAIP +T I VG+TIAIRVRIFG+WAVAFDRIVDV+ E DG+ +RFG SVGTL +HPEQGEERFSIEID +  VDYEVAAFFRPNT+AAK+AWP L RRFN+FR+QSA+AM
Sbjct:    2 EAIPSDTSITVGNTIAIRVRIFGVWAVAFDRIVDVYGEQDGECRRFGFSVGTLTEHPEQGEERFSIEIDREDHVDYEVAAFFRPNTLAAKIAWPVLHRRFNRFRNQSAEAM 112          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig74613.17655.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D9JCP9_9BACT4.880e-9974.07DUF1990 domain-containing protein n=2 Tax=Rhodopir... [more]
A0A2G1W2S8_9BACT3.430e-9879.31DUF1990 domain-containing protein n=1 Tax=Rhodopir... [more]
Q7UV48_RHOBA1.040e-9571.81DUF1990 domain-containing protein n=5 Tax=Rhodopir... [more]
M2B5D1_9BACT7.020e-9270.81Uncharacterized conserved protein UCP010260 n=1 Ta... [more]
UPI001E3C47E23.790e-7771.97DUF1990 domain-containing protein n=1 Tax=Rhodopir... [more]
M5S1K9_9BACT1.070e-6875.18DUF1990 domain-containing protein n=1 Tax=Rhodopir... [more]
A0A5C6BDJ1_9BACT2.660e-6452.38DUF1990 domain-containing protein n=1 Tax=Stieleri... [more]
A0A5M6CVC5_9BACT1.450e-6051.35DUF1990 domain-containing protein n=1 Tax=Roseicon... [more]
A0A517MD82_9BACT3.990e-5648.68DUF1990 domain-containing protein n=1 Tax=Roseimar... [more]
F2B257_RHOBT5.200e-5376.58DUF1990 domain-containing protein n=2 Tax=Rhodopir... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig74613contigM-pyrifera_M_contig74613:438..1037 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score287.7
Seed ortholog evalue4.1e-75
Seed eggNOG ortholog243090.RB2878
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Domain of unknown function (DUF1990)
EggNOG OGs2IZUS@203682,COG4762@1,COG4762@2
COG Functional cat.S
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
Exons1
Model size600
Cds size600
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816644.7635133-CDS-M-pyrifera_M_contig74613:437..10371622816644.7635133-CDS-M-pyrifera_M_contig74613:437..1037Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74613 438..1037 -
1692278095.7555652-CDS-M-pyrifera_M_contig74613:437..10371692278095.7555652-CDS-M-pyrifera_M_contig74613:437..1037Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74613 438..1037 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig74613.17655.1prot_M-pyrifera_M_contig74613.17655.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig74613 438..1037 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig74613.17655.1

>prot_M-pyrifera_M_contig74613.17655.1 ID=prot_M-pyrifera_M_contig74613.17655.1|Name=mRNA_M-pyrifera_M_contig74613.17655.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=200bp
EAPLFSLTKPTHRQILRSISEQSQSPLNYPDVGATRSRFPDGYEHHRHRL
VLGHGSGVFESAKVALENWQQFDVGWAEAIPKETPIRVGSTIAIRVRIFG
LWAVAFDRIVDVHSESDGDRQRFGLSVGTLMDHPEQGEERFSIEIDHDGQ
VDYEVAAFFRPNTVAAKMAWPYLRRRFNQFRDQSADAMRRQVDSVDLSQ*
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mRNA from alignment at M-pyrifera_M_contig74613:438..1037-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig74613.17655.1 ID=mRNA_M-pyrifera_M_contig74613.17655.1|Name=mRNA_M-pyrifera_M_contig74613.17655.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=600bp|location=Sequence derived from alignment at M-pyrifera_M_contig74613:438..1037- (Macrocystis pyrifera P11B4 male)
GAGGCGCCCTTGTTCAGCCTGACCAAACCGACTCACCGTCAGATTCTTCG ATCGATTTCGGAGCAATCTCAGTCACCGCTGAATTATCCAGACGTGGGAG CGACGCGCAGCAGGTTTCCTGATGGGTATGAGCACCATCGTCATCGCTTG GTGCTGGGCCATGGGTCAGGCGTCTTCGAGAGTGCGAAGGTGGCTTTGGA AAATTGGCAACAGTTTGATGTTGGATGGGCCGAAGCGATTCCCAAAGAGA CACCCATTCGGGTCGGAAGCACGATCGCGATCCGTGTCAGGATCTTTGGA TTGTGGGCAGTTGCATTCGATCGAATCGTTGACGTTCACAGTGAAAGCGA TGGGGATCGGCAACGGTTCGGACTTTCTGTTGGGACGCTGATGGACCATC CCGAGCAAGGCGAGGAACGATTTTCAATCGAGATTGACCACGATGGCCAA GTGGACTACGAAGTCGCAGCTTTCTTTCGACCAAACACAGTCGCCGCGAA AATGGCTTGGCCCTACCTGCGCCGAAGATTCAACCAGTTTCGAGATCAGT CCGCGGATGCGATGAGGCGACAAGTCGATTCGGTCGACCTATCCCAATGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig74613:438..1037-

>mRNA_M-pyrifera_M_contig74613.17655.1 ID=mRNA_M-pyrifera_M_contig74613.17655.1|Name=mRNA_M-pyrifera_M_contig74613.17655.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1200bp|location=Sequence derived from alignment at M-pyrifera_M_contig74613:438..1037- (Macrocystis pyrifera P11B4 male)
GAGGCGCCCTTGTTCAGCCTGACCAAACCGACTCACCGTCAGATTCTTCG
ATCGATTTCGGAGCAATCTCAGTCACCGCTGAATTATCCAGACGTGGGAG
CGACGCGCAGCAGGTTTCCTGATGGGTATGAGCACCATCGTCATCGCTTG
GTGCTGGGCCATGGGTCAGGCGTCTTCGAGAGTGCGAAGGTGGCTTTGGA
AAATTGGCAACAGTTTGATGTTGGATGGGCCGAAGCGATTCCCAAAGAGA
CACCCATTCGGGTCGGAAGCACGATCGCGATCCGTGTCAGGATCTTTGGA
TTGTGGGCAGTTGCATTCGATCGAATCGTTGACGTTCACAGTGAAAGCGA
TGGGGATCGGCAACGGTTCGGACTTTCTGTTGGGACGCTGATGGACCATC
CCGAGCAAGGCGAGGAACGATTTTCAATCGAGATTGACCACGATGGCCAA
GTGGACTACGAAGTCGCAGCTTTCTTTCGACCAAACACAGTCGCCGCGAA
AATGGCTTGGCCCTACCTGCGCCGAAGATTCAACCAGTTTCGAGATCAGT
CCGCGGATGCGATGAGGCGACAAGTCGATTCGGTCGACCTATCCCAATGA
GAGGCGCCCTTGTTCAGCCTGACCAAACCGACTCACCGTCAGATTCTTCG
ATCGATTTCGGAGCAATCTCAGTCACCGCTGAATTATCCAGACGTGGGAG
CGACGCGCAGCAGGTTTCCTGATGGGTATGAGCACCATCGTCATCGCTTG
GTGCTGGGCCATGGGTCAGGCGTCTTCGAGAGTGCGAAGGTGGCTTTGGA
AAATTGGCAACAGTTTGATGTTGGATGGGCCGAAGCGATTCCCAAAGAGA
CACCCATTCGGGTCGGAAGCACGATCGCGATCCGTGTCAGGATCTTTGGA
TTGTGGGCAGTTGCATTCGATCGAATCGTTGACGTTCACAGTGAAAGCGA
TGGGGATCGGCAACGGTTCGGACTTTCTGTTGGGACGCTGATGGACCATC
CCGAGCAAGGCGAGGAACGATTTTCAATCGAGATTGACCACGATGGCCAA
GTGGACTACGAAGTCGCAGCTTTCTTTCGACCAAACACAGTCGCCGCGAA
AATGGCTTGGCCCTACCTGCGCCGAAGATTCAACCAGTTTCGAGATCAGT
CCGCGGATGCGATGAGGCGACAAGTCGATTCGGTCGACCTATCCCAATGA
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