mRNA_M-pyrifera_M_contig74128.17552.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig74128.17552.1
Unique NamemRNA_M-pyrifera_M_contig74128.17552.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: UPI00188601C7 (probable proline--tRNA ligase, mitochondrial n=1 Tax=Syngnathus acus TaxID=161584 RepID=UPI00188601C7)

HSP 1 Score: 130 bits (326), Expect = 1.190e-32
Identity = 69/160 (43.12%), Postives = 96/160 (60.00%), Query Frame = 1
Query:    1 VEAAAGDRCPANGCD-GTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKER-SALEMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEEVSTTTTDHT------DIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            VE    +R     C  GTL   +G+EVGH FYLG KYS  FNA     + + S  EMGCYGLG +RI++AAVE    D+ ++WP  +APY+ C++P  K   A  AAAM E    T   T      ++V DDR ++++GK+L +A+RLGYP V+++   A
Sbjct:  291 VETVVSNRTECPQCKTGTLTESKGIEVGHTFYLGKKYSDMFNAAFSNAQNKPSVAEMGCYGLGVTRILAAAVEVMSTDENIRWPGLLAPYQVCVLPPKKGSKAAEAAAMAEELVHTLGQTLPRLRGEVVFDDRTQMTIGKRLKDAKRLGYPYVVVVGQGA 450          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: A0A3Q3LFV9_9TELE (Proline--tRNA ligase n=2 Tax=Mastacembelus armatus TaxID=205130 RepID=A0A3Q3LFV9_9TELE)

HSP 1 Score: 129 bits (325), Expect = 1.550e-32
Identity = 69/160 (43.12%), Postives = 98/160 (61.25%), Query Frame = 1
Query:    1 VEAAAGDRCPANGCD-GTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKER-SALEMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEEVSTTTTDHT------DIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            VE    DRC    C  GTLV  RG+EVGH FYLG KYS  F+A     + + S  EMGCYGLG +RI++AA+E    ++G++WP  +APY+ C++P  K      AAA+ E    +   T      ++VLDDR ++++GK+L +A RLGYP V+++   A
Sbjct:  285 VETLTSDRCRCPQCKTGTLVESRGIEVGHTFYLGKKYSYVFSATFNNPQNKYSVAEMGCYGLGVTRILAAAIEVMSTEEGIRWPGLLAPYQVCVLPPKKGSKVGEAAALAEDLVHSLSETLPHLRGEVVLDDRTQMTIGKRLKDASRLGYPYVIVVGQGA 444          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: H2VE84_TAKRU (Proline--tRNA ligase n=5 Tax=Tetraodontidae TaxID=31031 RepID=H2VE84_TAKRU)

HSP 1 Score: 129 bits (325), Expect = 1.620e-32
Identity = 69/157 (43.95%), Postives = 101/157 (64.33%), Query Frame = 1
Query:   25 CPANGCDGTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKERSAL-EMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAM-EEVSTTTTD-----HTDIVLDDRKELSVGKKLSEAERLGYPLVLILSANAETSSTK 474
            CP  G  GTLV  +G+EVGH FYLG KYS+ FNA     + + A+ EMGCYGLG +RI++AA+E    ++G++WP  +APY+ C+VP  K   A  AA + EE+  T  D       ++VLDDR ++++GK+L +A +LGYP V+++   A    T+
Sbjct:  299 CPQCGT-GTLVESKGIEVGHTFYLGKKYSNVFNATFSNAQNKLAVAEMGCYGLGVTRILAAAIEVMSTEEGIRWPGLLAPYQVCVVPPKKGSKADKAAVIAEELVQTLADALPLLRGEVVLDDRTQMTIGKRLKDASKLGYPYVIVVGQGALEEPTR 454          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: A0A8M1HCR6_BETSP (probable proline--tRNA ligase, mitochondrial n=1 Tax=Betta splendens TaxID=158456 RepID=A0A8M1HCR6_BETSP)

HSP 1 Score: 128 bits (322), Expect = 6.740e-32
Identity = 74/162 (45.68%), Postives = 105/162 (64.81%), Query Frame = 1
Query:    1 VEAAAGDRCPANGCD-GTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKERSAL-EMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVP--KG-KIDPAVLAAAMEEVSTT---TTDHT--DIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            VE  + D      C  GTLV  +G+EVGH FYLG KYSS FNA     + +S++ EMGC+GLG +RI++AA+E    ++G+ WP  IAPY+ C+VP  KG K+D A L A  E++  T   T  H   ++VLDDR ++++GK+L +A RLGYP V+++   A
Sbjct:  333 VETISSDTTHCPQCSTGTLVESKGIEVGHTFYLGKKYSSIFNATFSNAQNKSSIAEMGCFGLGVTRILAAAIEVLSTEEGIHWPGLIAPYQVCVVPPKKGSKVDEAALQA--EQLVHTLGETLPHLRGEVVLDDRTQMTIGKRLKDASRLGYPYVIVVGQGA 492          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: A0A6P7Y3X3_9AMPH (Proline--tRNA ligase n=1 Tax=Microcaecilia unicolor TaxID=1415580 RepID=A0A6P7Y3X3_9AMPH)

HSP 1 Score: 127 bits (319), Expect = 9.980e-32
Identity = 64/146 (43.84%), Postives = 94/146 (64.38%), Query Frame = 1
Query:   25 CPANGCDGTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKERSAL-EMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVP-----KGKIDPAVLAAAMEEVSTTTTD-HTDIVLDDRKELSVGKKLSEAERLGYPLVLI 441
            CPA  C G L   +G+E+GH FYLGTKYSSSFNA     + +  L EMGCYGLG +R+++AA+E    +  ++WP  IAPY+ C +P     K +   A++ A  ++++  T     D++LDDR   S+GK+L +A+RLGYP V++
Sbjct:  286 CPA--CQGKLAETKGIEIGHTFYLGTKYSSSFNAAYSNAQNQPVLAEMGCYGLGVTRMLAAAIEVLSTEDSIRWPGLIAPYQVCFIPPKKGSKEESATALVEALYDDITEATPQLKGDMLLDDRSHFSIGKRLKDAKRLGYPYVIV 429          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: UPI001C35892D ((large yellow croaker) hypothetical protein n=1 Tax=Larimichthys crocea TaxID=215358 RepID=UPI001C35892D)

HSP 1 Score: 129 bits (323), Expect = 1.320e-31
Identity = 70/160 (43.75%), Postives = 97/160 (60.62%), Query Frame = 1
Query:    1 VEAAAGDRCPANGCD-GTLVSQRGMEVGHAFYLGTKYSSSFNAG-IGFGKERSALEMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEEVSTTTTDHT------DIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            VE  + DR     C  GTLV  +G+EVGH FYLG KYS+ FNA  I    + S  EMGCYGLG +RI++AA+E    ++G++WP  +APY+ CI+P  K      AA + E    T   T      ++VLDDR + ++GK+L +A RLGYP V+++   A
Sbjct:  384 VETMSSDRTDCPQCKTGTLVESKGIEVGHTFYLGKKYSNVFNAAFINAQNKPSVAEMGCYGLGVTRILAAAIEVMSTEEGIRWPGLLAPYQVCILPPKKGSKVDEAAVLAEELVHTLGETFSGLRGEVVLDDRTQATIGKRLKDANRLGYPYVIVVGQGA 543          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: A0A3B4UGG1_SERDU (Proline--tRNA ligase n=1 Tax=Seriola dumerili TaxID=41447 RepID=A0A3B4UGG1_SERDU)

HSP 1 Score: 126 bits (317), Expect = 1.520e-31
Identity = 69/166 (41.57%), Postives = 100/166 (60.24%), Query Frame = 1
Query:    1 VEAAAGDRCPANGCD-GTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKER-SALEMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEEVSTTTTD--HT----------DIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            VE  + DR     C  GTLV  RG+EVGH FYLG KYS  FNA     + + S  EMGCYGLG +RI++AA+E    ++G++WP  +APY+ C++P  K       + M+EV+    +  HT          ++VLDDR ++++GK+L +A RLGYP V+++   A
Sbjct:  255 VETMSSDRTDCPQCKTGTLVESRGIEVGHTFYLGKKYSQVFNATFSNAQNKPSVAEMGCYGLGVTRILAAAIEVMSTEEGIRWPGLLAPYQVCVLPPKK------GSKMDEVAVLADNLVHTLGEALPGLRGEVVLDDRTQMTIGKRLKDASRLGYPYVIVVGQGA 414          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: UPI000C6F936E (probable proline--tRNA ligase, mitochondrial n=1 Tax=Seriola lalandi dorsalis TaxID=1841481 RepID=UPI000C6F936E)

HSP 1 Score: 124 bits (311), Expect = 1.540e-31
Identity = 63/144 (43.75%), Postives = 90/144 (62.50%), Query Frame = 1
Query:   46 GTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKER-SALEMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEEVSTTTTDHT------DIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            GTLV  RG+EVGH FYLG KYS  FNA     + + S  EMGCYGLG +RI++AA+E    +KG++WP  +APY+ C++P  K       A + +    T   T      ++VLDDR ++++GK+L +A RLGYP V+++   A
Sbjct:  147 GTLVESRGIEVGHTFYLGKKYSQVFNATFSNVQNKPSVAEMGCYGLGVTRILAAAIEVMSTEKGIRWPGLLAPYQVCVLPPKKGSKMDEVAVLADNLVHTLGETLPGLRGEVVLDDRTQMTIGKRLKDASRLGYPYVIVVGQGA 290          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: A0A667YHZ7_9TELE (Proline--tRNA ligase n=1 Tax=Myripristis murdjan TaxID=586833 RepID=A0A667YHZ7_9TELE)

HSP 1 Score: 127 bits (318), Expect = 1.620e-31
Identity = 67/156 (42.95%), Postives = 95/156 (60.90%), Query Frame = 1
Query:    1 VEAAAGDRCPANGCD-GTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKERSAL-EMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEEVSTTTTDHT------DIVLDDRKELSVGKKLSEAERLGYPLVLIL 444
            VE  +  R     C  GTLV  +G+EVGH FYLGTKYS  FNA     + +  + EMGCYGLG +RI++AA+E    ++G++WP  +APY+ C+VP  K      AA + E    T   T      ++VLDDR  +++GK+L +A RLGYP V+++
Sbjct:  289 VETVSAGRTDCPQCKTGTLVESKGIEVGHTFYLGTKYSHIFNATFSNAQNKPTIAEMGCYGLGVTRILAAAIEVMSTEEGIRWPGLLAPYQVCVVPPKKGSKVDEAAGLAEELVHTFGETLPCLRGEVVLDDRTNMTIGKRLKDASRLGYPYVVVI 444          
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Match: UPI0003C4467E (LOW QUALITY PROTEIN: probable proline--tRNA ligase, mitochondrial n=1 Tax=Pelodiscus sinensis TaxID=13735 RepID=UPI0003C4467E)

HSP 1 Score: 125 bits (314), Expect = 3.940e-31
Identity = 67/151 (44.37%), Postives = 90/151 (59.60%), Query Frame = 1
Query:   25 CPANGCDGTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKERSAL-EMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDPAVLAAAMEE------VSTTTTDHTDIVLDDRKELSVGKKLSEAERLGYPLVLILSANA 456
            CPA  C G L   +G+EVGH FYLGTKYSS FN      + +  L EMGCYGLG +RI++A++E    +  ++WP  IAPY+ C++P  K     + AA+ +      V        D+VLDDR  L++GK+L +A RLGYP VLI    A
Sbjct:  263 CPA--CKGKLAETKGIEVGHTFYLGTKYSSVFNVIFHTAENKPVLTEMGCYGLGITRILAASIEVLSTEDSIRWPTLIAPYQVCLIPPKKGSKEEIGAAIMDNLYDSIVEAVPQIKGDVVLDDRTHLTIGKRLKDANRLGYPYVLIAGKKA 411          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig74128.17552.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00188601C71.190e-3243.13probable proline--tRNA ligase, mitochondrial n=1 T... [more]
A0A3Q3LFV9_9TELE1.550e-3243.13Proline--tRNA ligase n=2 Tax=Mastacembelus armatus... [more]
H2VE84_TAKRU1.620e-3243.95Proline--tRNA ligase n=5 Tax=Tetraodontidae TaxID=... [more]
A0A8M1HCR6_BETSP6.740e-3245.68probable proline--tRNA ligase, mitochondrial n=1 T... [more]
A0A6P7Y3X3_9AMPH9.980e-3243.84Proline--tRNA ligase n=1 Tax=Microcaecilia unicolo... [more]
UPI001C35892D1.320e-3143.75(large yellow croaker) hypothetical protein n=1 Ta... [more]
A0A3B4UGG1_SERDU1.520e-3141.57Proline--tRNA ligase n=1 Tax=Seriola dumerili TaxI... [more]
UPI000C6F936E1.540e-3143.75probable proline--tRNA ligase, mitochondrial n=1 T... [more]
A0A667YHZ7_9TELE1.620e-3142.95Proline--tRNA ligase n=1 Tax=Myripristis murdjan T... [more]
UPI0003C4467E3.940e-3144.37LOW QUALITY PROTEIN: probable proline--tRNA ligase... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig74128contigM-pyrifera_M_contig74128:338..811 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score130.6
Seed ortholog evalue6.6e-28
Seed eggNOG ortholog31033.ENSTRUP00000047529
Preferred namePARS2
KEGG rclassRC00055,RC00523
KEGG koko:K01881
KEGG ReactionR03661
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004812,GO:0004827,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006418,GO:0006433,GO:0006518,GO:0006520,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0016070,GO:0016874,GO:0016875,GO:0019538,GO:0019752,GO:0034641,GO:0034645,GO:0034660,GO:0043038,GO:0043039,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0071704,GO:0090304,GO:0140098,GO:0140101,GO:1901360,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.prolyl-tRNA synthetase (mitochondrial)
EggNOG OGs38HAU@33154,3BD03@33208,3CSIY@33213,485DN@7711,48V2M@7742,49Q7U@7898,COG0442@1,KOG2324@2759
EC6.1.1.15
COG Functional cat.J
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016
Exons1
Model size474
Cds size474
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816639.139142-CDS-M-pyrifera_M_contig74128:337..8111622816639.139142-CDS-M-pyrifera_M_contig74128:337..811Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74128 338..811 +
1692278092.4929686-CDS-M-pyrifera_M_contig74128:337..8111692278092.4929686-CDS-M-pyrifera_M_contig74128:337..811Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74128 338..811 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig74128.17552.1prot_M-pyrifera_M_contig74128.17552.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig74128 338..811 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig74128.17552.1

>prot_M-pyrifera_M_contig74128.17552.1 ID=prot_M-pyrifera_M_contig74128.17552.1|Name=mRNA_M-pyrifera_M_contig74128.17552.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=158bp
VEAAAGDRCPANGCDGTLVSQRGMEVGHAFYLGTKYSSSFNAGIGFGKER
SALEMGCYGLGTSRIISAAVEHYHDDKGLKWPPSIAPYKACIVPKGKIDP
AVLAAAMEEVSTTTTDHTDIVLDDRKELSVGKKLSEAERLGYPLVLILSA
NAETSSTK
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mRNA from alignment at M-pyrifera_M_contig74128:338..811+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig74128.17552.1 ID=mRNA_M-pyrifera_M_contig74128.17552.1|Name=mRNA_M-pyrifera_M_contig74128.17552.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=474bp|location=Sequence derived from alignment at M-pyrifera_M_contig74128:338..811+ (Macrocystis pyrifera P11B4 male)
GTGGAAGCGGCTGCGGGCGACCGCTGTCCTGCCAACGGGTGTGACGGCAC GTTGGTCTCTCAGAGAGGAATGGAGGTTGGCCATGCGTTCTACCTTGGTA CGAAGTACTCCTCCAGCTTCAACGCTGGGATTGGCTTCGGGAAGGAGCGC AGCGCGCTCGAGATGGGCTGCTACGGTCTCGGCACCTCCCGCATCATTTC TGCAGCAGTTGAACACTACCATGATGACAAGGGCTTGAAGTGGCCTCCCT CTATTGCCCCTTACAAGGCCTGTATCGTGCCCAAGGGAAAGATCGACCCT GCTGTCCTCGCGGCTGCCATGGAGGAGGTGTCCACCACCACTACAGACCA CACTGACATTGTGTTGGATGACAGGAAGGAGCTCTCTGTGGGCAAGAAGC TGAGTGAAGCTGAGCGACTTGGTTATCCTCTCGTCTTGATACTCTCTGCC AATGCAGAAACGAGCTCCACGAAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig74128:338..811+

>mRNA_M-pyrifera_M_contig74128.17552.1 ID=mRNA_M-pyrifera_M_contig74128.17552.1|Name=mRNA_M-pyrifera_M_contig74128.17552.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=948bp|location=Sequence derived from alignment at M-pyrifera_M_contig74128:338..811+ (Macrocystis pyrifera P11B4 male)
GTGGAAGCGGCTGCGGGCGACCGCTGTCCTGCCAACGGGTGTGACGGCAC
GTTGGTCTCTCAGAGAGGAATGGAGGTTGGCCATGCGTTCTACCTTGGTA
CGAAGTACTCCTCCAGCTTCAACGCTGGGATTGGCTTCGGGAAGGAGCGC
AGCGCGCTCGAGATGGGCTGCTACGGTCTCGGCACCTCCCGCATCATTTC
TGCAGCAGTTGAACACTACCATGATGACAAGGGCTTGAAGTGGCCTCCCT
CTATTGCCCCTTACAAGGCCTGTATCGTGCCCAAGGGAAAGATCGACCCT
GCTGTCCTCGCGGCTGCCATGGAGGAGGTGTCCACCACCACTACAGACCA
CACTGACATTGTGTTGGATGACAGGAAGGAGCTCTCTGTGGGCAAGAAGC
TGAGTGAAGCTGAGCGACTTGGTTATCCTCTCGTCTTGATACTCTCTGCC
AATGCAGAAACGAGCTCCACGAAGGTGGAAGCGGCTGCGGGCGACCGCTG
TCCTGCCAACGGGTGTGACGGCACGTTGGTCTCTCAGAGAGGAATGGAGG
TTGGCCATGCGTTCTACCTTGGTACGAAGTACTCCTCCAGCTTCAACGCT
GGGATTGGCTTCGGGAAGGAGCGCAGCGCGCTCGAGATGGGCTGCTACGG
TCTCGGCACCTCCCGCATCATTTCTGCAGCAGTTGAACACTACCATGATG
ACAAGGGCTTGAAGTGGCCTCCCTCTATTGCCCCTTACAAGGCCTGTATC
GTGCCCAAGGGAAAGATCGACCCTGCTGTCCTCGCGGCTGCCATGGAGGA
GGTGTCCACCACCACTACAGACCACACTGACATTGTGTTGGATGACAGGA
AGGAGCTCTCTGTGGGCAAGAAGCTGAGTGAAGCTGAGCGACTTGGTTAT
CCTCTCGTCTTGATACTCTCTGCCAATGCAGAAACGAGCTCCACGAAG
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