mRNA_M-pyrifera_M_contig73749.17476.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig73749.17476.1
Unique NamemRNA_M-pyrifera_M_contig73749.17476.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: A0A2B4SPX9_STYPI (Haloacid dehalogenase-like hydrolase domain-containing protein n=2 Tax=Pocilloporidae TaxID=46729 RepID=A0A2B4SPX9_STYPI)

HSP 1 Score: 109 bits (273), Expect = 7.910e-26
Identity = 73/199 (36.68%), Postives = 112/199 (56.28%), Query Frame = 1
Query:   37 GVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAPF-PPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHFPTLHPLTTLL 630
            GV+FDLD TL    ++F ++R+ ++CPK +  LEF  +   +EK    + VV  EEE GR+  +L PG  E+L  L  + +  AL+TRN +A+V+ FL L+         LT      F PPKPDPAP LHI  +    +  ++V+ VGD + D++CG++AG+ T L+           +F   ADF+  +L  + TL+
Sbjct:   47 GVVFDLDGTLTVPVLDFAKLRKELQCPKGVDFLEFCNSKIGQEKEAALQIVVKFEEE-GRQNTKLQPGVFELLKFLSTSGLKRALITRNLQASVDQFLALMGHPDDYGGPLTHSLTREFTPPKPDPAPLLHICRDWG--VHPKNVVMVGDHLHDIQCGKSAGSVTILLNDS-----TNGNFKKDADFNVDSLSEIITLI 237          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: UPI0009E18C45 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 n=1 Tax=Orbicella faveolata TaxID=48498 RepID=UPI0009E18C45)

HSP 1 Score: 109 bits (273), Expect = 7.910e-26
Identity = 74/200 (37.00%), Postives = 110/200 (55.00%), Query Frame = 1
Query:   37 GVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALT--LHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHFPTLHPLTTLL 630
            G++FDLD TL    ++F ++R  ++CPK + IL+F  +    EK    + +V E EE GR+  +L PG  E+L  L    +  AL+TRN +A+V+ FL+LL         LT  L RD   PPKPDPAP LHI  E    +    V+ VGD ++D++CG+ AG+ T L+            F   ADF+  +L+ +  L+
Sbjct:   47 GIVFDLDGTLTLPVLDFAKLRSDLQCPKGVDILQFCNSKSGSEKEMALQ-LVEEFEEEGRKNTKLQPGVFELLKFLSQCGLKRALITRNLQASVDQFLDLLGHPDNYGGPLTHFLTRDFT-PPKPDPAPLLHICKEWG--VHPRSVVMVGDHLNDIQCGKDAGSVTVLLNDSKN-----GDFKKYADFNVDSLNEIVNLI 237          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: A0A813Y665_9BILA (Hypothetical protein n=1 Tax=Didymodactylos carnosus TaxID=1234261 RepID=A0A813Y665_9BILA)

HSP 1 Score: 102 bits (253), Expect = 5.810e-23
Identity = 67/172 (38.95%), Postives = 101/172 (58.72%), Query Frame = 1
Query:   28 RARGVIFDLDSTLLRT-KINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVG 540
            R RGVIFD+D TL R   I+F R+RER        I+EF+   ++E  R +   +++EEEE G +  EL+P   ++   L++  I  ALLTRN++  +   +EL+    +  F++ L R    PPKP P  A+H + +    +   +VL VGD++DD+K G AAGTKT L+G
Sbjct:   15 RIRGVIFDMDGTLTRPGAIDFARMRERCGIKPGGDIIEFVNN-QSEPLRSKLNEILIEEEEFGLKRMELMPDIKQLFDYLIENNINRALLTRNNDTIMNRTVELMNYAKQEPFSVMLSRTFS-PPKPHPD-AIHHICQRWK-LNVNEVLMVGDSLDDIKAGAAAGTKTVLIG 182          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: A0A2E8VL99_9GAMM (Phosphatase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E8VL99_9GAMM)

HSP 1 Score: 95.9 bits (237), Expect = 6.100e-21
Identity = 66/182 (36.26%), Postives = 102/182 (56.04%), Query Frame = 1
Query:    7 GTVAKAWRA---RGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGP 543
            G +   WR+   R VIFDLD TL+RT+++F  +R ++  P+ +GILE +E++    ++    +V   E +     A  +PGA ++L++L  AE+P  ++TRN   A    L  L      ++ L    D P  PKPDP+  L  L++A + +P  DVLFVGD + DL+   AAG  +CL  P
Sbjct:    5 GMLRSPWRSPVIRAVIFDLDGTLVRTRLDFAGLRRQLGFPEGVGILEHIESLTGVARQSAXAAVEAHEMDCAAT-ATWMPGAXDLLASLRGAELPWGVVTRNTRQAARVVLGRLTDH---SYTLLAREDCP--PKPDPSGPLS-LAQAWN-VPPRDVLFVGDFIYDLQAAHAAGMPSCLYAP 178          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: UPI001CF43264 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 n=1 Tax=Acropora millepora TaxID=45264 RepID=UPI001CF43264)

HSP 1 Score: 96.3 bits (238), Expect = 9.110e-21
Identity = 68/203 (33.50%), Postives = 105/203 (51.72%), Query Frame = 1
Query:   28 RARGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPN--FALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHFPTLHPLTTLL 630
            +  G +FDLD TL    ++F  +R R++CP  + IL F  +   ++K K F  VV E EE GR+  +L PG  E+L  L    +  AL+TRN + +V+    LL         F  +L RD   P KPDPA  L+I  +    +  + V+ VGD + D++CG++AG+ T L+            F   ADF+  +L  + +L+
Sbjct:   34 QVHGFVFDLDGTLTLPVLDFAELRRRLQCPNGVDILTFCNSKSGKDKEKAFH-VVEEFEEEGRQNTKLQPGVCELLKFLTQCGLKRALITRNLQPSVDQLFHLLGDPDNYGGPFTHSLTRDFT-PTKPDPASLLYICKQWG--VHPKSVVMVGDQLQDIQCGKSAGSVTILLNDSKN-----GDFKKQADFNVDSLDAIISLI 227          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: UPI001B39DAF8 (HAD family hydrolase n=1 Tax=Pseudoalteromonas sp. MMG010 TaxID=2822685 RepID=UPI001B39DAF8)

HSP 1 Score: 95.1 bits (235), Expect = 1.050e-20
Identity = 66/199 (33.17%), Postives = 110/199 (55.28%), Query Frame = 1
Query:   34 RGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHFPTLHPLTTLL 630
            +GVIFDLD TL+ ++++F  I+ ++ CPK   +L +++++ +   R+E  ++V + E L  + A LLPG  + +S L +  IP A++TRN E A    L+L    +  +  LT   D P   KPDP     I S  +  I ++++++VGD + D++    A  + CL  PG        +FAS AD+    +H L TL+
Sbjct:    9 KGVIFDLDGTLVSSELDFALIKAQVGCPKDKDLLAYIDSLPSPYMREEAINIVHQHELLDAQSATLLPGVAQAISTLHNNNIPMAIVTRNFEKAAT--LKLKNNPLPIDIVLT-REDGPA--KPDPTALKSIAS--LWNIHSDNIIYVGDYLYDIQAAHNANMRACLYVPGQA-----PTFASEADYVIHNMHELVTLV 195          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: A0A317FPI0_9GAMM (HAD family hydrolase n=20 Tax=Pseudoalteromonadaceae TaxID=267888 RepID=A0A317FPI0_9GAMM)

HSP 1 Score: 93.2 bits (230), Expect = 5.700e-20
Identity = 67/200 (33.50%), Postives = 113/200 (56.50%), Query Frame = 1
Query:   34 RGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRN-DEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHFPTLHPLTTLL 630
            +GVIFDLD TL+ +++NF  I+ ++ CP  + +LE++E + +   R+E  ++V + E L  + A LLPG +E ++AL    IP A++TRN D+AA       L+ +  P    T+      P KPDP+ AL +++   S +P ++V++VGD + D++    A  + CL  P    + P  ++A  ADF       L +L+
Sbjct:    9 KGVIFDLDGTLVSSELNFSLIKAQVGCPAELDLLEYIEQLPSPYMREEAMNIVHQHELLDAQTATLLPGVVEAVNALRAKNIPMAIVTRNFDKAAT------LKLQHNPLPIETVLTRTEAPAKPDPS-ALLMIARQWS-MPVDNVIYVGDYLYDIQAAHNAKMRACLYAPD---VTP--AYAEQADFVIHAFDELVSLV 195          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: UPI001EEFD90E (HAD-IA family hydrolase n=1 Tax=Marinobacterium sediminicola TaxID=518898 RepID=UPI001EEFD90E)

HSP 1 Score: 92.0 bits (227), Expect = 1.230e-19
Identity = 71/201 (35.32%), Postives = 107/201 (53.23%), Query Frame = 1
Query:   28 RARGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAP---FPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADF---HFPTLH 612
            + +GVIFDLD TL+ + ++F+ I++ I+CP    +L FLET+  E K+ E  +++   E    +  E LPGA E ++  +   IP A++TRN        L   R ++K N A+ + R        PKPDP   L I  +    +PA  +L VGD   DL+ GR A  K+CL+   +   LP   ++ LAD+   HF  LH
Sbjct:    3 QVQGVIFDLDGTLVSSSLDFKAIKQEIDCPPEADVLTFLETLP-EHKQLEAMAIIHRHEVQDAQQCEWLPGAQEFVNTCIKHSIPMAIVTRNS-------LHSSRVKIKRN-AIPIERVITRENSKPKPDPTALLQIADDFA--LPAHSILMVGDYRYDLEAGRNANMKSCLI---NYTTLP--DYSHLADYTFEHFGLLH 187          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: A0A0U4V9J5_9GAMM (Phosphatase n=1 Tax=Rheinheimera sp. F8 TaxID=1763998 RepID=A0A0U4V9J5_9GAMM)

HSP 1 Score: 91.7 bits (226), Expect = 2.110e-19
Identity = 74/205 (36.10%), Postives = 114/205 (55.61%), Query Frame = 1
Query:   34 RGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCL-VGPGHG-------FLLPGESFASLADFHFPTLHPLTT 624
            +GVIFDLD TL+ +K++F  + +++  P    ILE L T+    + +   +++ + E  G + A+ +PGA E+L+ L   +IP A+LTRN  +A +H L+ L   +  +  LT   DAP   KPDP   L I++      PA D+LF+GD   DL   RAAG  +CL + P +G       F+L  + FA LA  +   L P+T+
Sbjct:    7 QGVIFDLDGTLVDSKLDFSLLCQQLGWPAGTPILEHLATITVRSEYQRAVTIIEQFELAGAQSADWMPGAAELLALLQQQQIPTAILTRNMRSATQHCLQRLGIAV--DLVLT-REDAPA--KPDPT-GLLIIASHWQKAPA-DLLFIGDFHFDLTTARAAGMPSCLYLNPDNGHYRDQADFVL--QHFAELAQRYQQKLVPITS 202          
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Match: A0A7C4UE78_9BACT (HAD family hydrolase n=1 Tax=Gemmataceae bacterium TaxID=2052164 RepID=A0A7C4UE78_9BACT)

HSP 1 Score: 91.3 bits (225), Expect = 2.960e-19
Identity = 71/201 (35.32%), Postives = 100/201 (49.75%), Query Frame = 1
Query:   28 RARGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLETVENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTRNDEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSFIPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHFPTLHPLTTLL 630
            R RGV+FDLD TL+  +++F  IRE I  P    +LE LE +   E  +   +++   E      A+LLPG  E    L    +  A+L+RN   +V+  L+       P   +    DAP+  KPDP   + I S     +P+ DV  +GD + D++ GR AG +T L+   HG  LP   FA LAD  FP    L  LL
Sbjct:    8 RLRGVVFDLDGTLVSQELDFPAIREEIGLPVGQPLLEALEQMTGNELERAL-AIIDRHERTAATTAKLLPGVREFTLWLEKQGVRQAVLSRNSRRSVQEVLQRWELAFDP---IVAREDAPY--KPDPGGIIQICSR-WGVVPS-DVAMIGDFLYDIEAGRRAGARTALIT--HGRDLP---FAHLADVTFPNFEELPDLL 195          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig73749.17476.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2B4SPX9_STYPI7.910e-2636.68Haloacid dehalogenase-like hydrolase domain-contai... [more]
UPI0009E18C457.910e-2637.00haloacid dehalogenase-like hydrolase domain-contai... [more]
A0A813Y665_9BILA5.810e-2338.95Hypothetical protein n=1 Tax=Didymodactylos carnos... [more]
A0A2E8VL99_9GAMM6.100e-2136.26Phosphatase n=1 Tax=Gammaproteobacteria bacterium ... [more]
UPI001CF432649.110e-2133.50haloacid dehalogenase-like hydrolase domain-contai... [more]
UPI001B39DAF81.050e-2033.17HAD family hydrolase n=1 Tax=Pseudoalteromonas sp.... [more]
A0A317FPI0_9GAMM5.700e-2033.50HAD family hydrolase n=20 Tax=Pseudoalteromonadace... [more]
UPI001EEFD90E1.230e-1935.32HAD-IA family hydrolase n=1 Tax=Marinobacterium se... [more]
A0A0U4V9J5_9GAMM2.110e-1936.10Phosphatase n=1 Tax=Rheinheimera sp. F8 TaxID=1763... [more]
A0A7C4UE78_9BACT2.960e-1935.32HAD family hydrolase n=1 Tax=Gemmataceae bacterium... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig73749contigM-pyrifera_M_contig73749:394..1026 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score98.6
Seed ortholog evalue3.7e-18
Seed eggNOG ortholog234831.PSM_B0060
Preferred namepgp
KEGG rclassRC00017
KEGG koko:K01091,ko:K07025
KEGG ReactionR01334
KEGG Pathwayko00630,ko01100,ko01110,ko01130,map00630,map01100,map01110,map01130
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Hydrolase phosphatase
EggNOG OGs1RHAA@1224,1S7HQ@1236,2Q0TV@267888,COG0546@1,COG0546@2
EC3.1.3.18
COG Functional cat.S
BiGG ReactioniJN678.slr0458
Best tax levelPseudoalteromonadaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size633
Cds size633
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816634.971963-CDS-M-pyrifera_M_contig73749:393..10261622816634.971963-CDS-M-pyrifera_M_contig73749:393..1026Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig73749 394..1026 -
1692278090.018708-CDS-M-pyrifera_M_contig73749:393..10261692278090.018708-CDS-M-pyrifera_M_contig73749:393..1026Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig73749 394..1026 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig73749.17476.1prot_M-pyrifera_M_contig73749.17476.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig73749 394..1026 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig73749.17476.1

>prot_M-pyrifera_M_contig73749.17476.1 ID=prot_M-pyrifera_M_contig73749.17476.1|Name=mRNA_M-pyrifera_M_contig73749.17476.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=211bp
MAGTVAKAWRARGVIFDLDSTLLRTKINFRRIRERIECPKAMGILEFLET
VENEEKRKEFESVVLEEEELGREGAELLPGAIEVLSALVDAEIPHALLTR
NDEAAVEHFLELLRAEMKPNFALTLHRDAPFPPKPDPAPALHILSEAMSF
IPAEDVLFVGDAVDDLKCGRAAGTKTCLVGPGHGFLLPGESFASLADFHF
PTLHPLTTLL*
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mRNA from alignment at M-pyrifera_M_contig73749:394..1026-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig73749.17476.1 ID=mRNA_M-pyrifera_M_contig73749.17476.1|Name=mRNA_M-pyrifera_M_contig73749.17476.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=633bp|location=Sequence derived from alignment at M-pyrifera_M_contig73749:394..1026- (Macrocystis pyrifera P11B4 male)
ATGGCTGGGACAGTTGCAAAAGCATGGAGGGCGAGAGGGGTGATTTTCGA CCTAGATAGCACTCTGCTGCGCACGAAGATTAATTTTCGAAGAATCCGCG AGAGAATTGAGTGTCCGAAGGCGATGGGAATCTTGGAATTCCTGGAAACA GTGGAGAATGAGGAAAAGAGGAAAGAGTTTGAAAGTGTGGTGTTGGAGGA GGAGGAGTTGGGGCGTGAAGGCGCAGAGTTGTTGCCGGGTGCGATTGAAG TGCTTTCTGCTTTGGTTGATGCAGAAATTCCACATGCATTGTTGACAAGG AATGATGAAGCTGCAGTGGAGCACTTTTTAGAGTTGCTTCGTGCAGAAAT GAAGCCCAATTTCGCACTCACTCTACATCGTGACGCCCCCTTCCCTCCAA AACCTGATCCAGCCCCTGCCCTTCACATTCTGAGCGAAGCAATGAGTTTC ATTCCAGCAGAAGACGTGTTGTTTGTTGGAGACGCAGTGGACGACTTGAA ATGCGGCAGAGCAGCGGGGACCAAGACTTGCTTGGTGGGTCCAGGACATG GCTTCCTCCTCCCTGGAGAATCCTTCGCTTCCCTCGCCGACTTTCACTTC CCCACCCTCCACCCGCTCACCACCCTCCTGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig73749:394..1026-

>mRNA_M-pyrifera_M_contig73749.17476.1 ID=mRNA_M-pyrifera_M_contig73749.17476.1|Name=mRNA_M-pyrifera_M_contig73749.17476.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1266bp|location=Sequence derived from alignment at M-pyrifera_M_contig73749:394..1026- (Macrocystis pyrifera P11B4 male)
ATGGCTGGGACAGTTGCAAAAGCATGGAGGGCGAGAGGGGTGATTTTCGA
CCTAGATAGCACTCTGCTGCGCACGAAGATTAATTTTCGAAGAATCCGCG
AGAGAATTGAGTGTCCGAAGGCGATGGGAATCTTGGAATTCCTGGAAACA
GTGGAGAATGAGGAAAAGAGGAAAGAGTTTGAAAGTGTGGTGTTGGAGGA
GGAGGAGTTGGGGCGTGAAGGCGCAGAGTTGTTGCCGGGTGCGATTGAAG
TGCTTTCTGCTTTGGTTGATGCAGAAATTCCACATGCATTGTTGACAAGG
AATGATGAAGCTGCAGTGGAGCACTTTTTAGAGTTGCTTCGTGCAGAAAT
GAAGCCCAATTTCGCACTCACTCTACATCGTGACGCCCCCTTCCCTCCAA
AACCTGATCCAGCCCCTGCCCTTCACATTCTGAGCGAAGCAATGAGTTTC
ATTCCAGCAGAAGACGTGTTGTTTGTTGGAGACGCAGTGGACGACTTGAA
ATGCGGCAGAGCAGCGGGGACCAAGACTTGCTTGGTGGGTCCAGGACATG
GCTTCCTCCTCCCTGGAGAATCCTTCGCTTCCCTCGCCGACTTTCACTTC
CCCACCCTCCACCCGCTCACCACCCTCCTGTGAATGGCTGGGACAGTTGC
AAAAGCATGGAGGGCGAGAGGGGTGATTTTCGACCTAGATAGCACTCTGC
TGCGCACGAAGATTAATTTTCGAAGAATCCGCGAGAGAATTGAGTGTCCG
AAGGCGATGGGAATCTTGGAATTCCTGGAAACAGTGGAGAATGAGGAAAA
GAGGAAAGAGTTTGAAAGTGTGGTGTTGGAGGAGGAGGAGTTGGGGCGTG
AAGGCGCAGAGTTGTTGCCGGGTGCGATTGAAGTGCTTTCTGCTTTGGTT
GATGCAGAAATTCCACATGCATTGTTGACAAGGAATGATGAAGCTGCAGT
GGAGCACTTTTTAGAGTTGCTTCGTGCAGAAATGAAGCCCAATTTCGCAC
TCACTCTACATCGTGACGCCCCCTTCCCTCCAAAACCTGATCCAGCCCCT
GCCCTTCACATTCTGAGCGAAGCAATGAGTTTCATTCCAGCAGAAGACGT
GTTGTTTGTTGGAGACGCAGTGGACGACTTGAAATGCGGCAGAGCAGCGG
GGACCAAGACTTGCTTGGTGGGTCCAGGACATGGCTTCCTCCTCCCTGGA
GAATCCTTCGCTTCCCTCGCCGACTTTCACTTCCCCACCCTCCACCCGCT
CACCACCCTCCTGTGA
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