mRNA_M-pyrifera_M_contig72886.17311.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig72886.17311.1
Unique NamemRNA_M-pyrifera_M_contig72886.17311.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A7S3D6Y8_9EUKA (Hypothetical protein n=1 Tax=Palpitomonas bilix TaxID=652834 RepID=A0A7S3D6Y8_9EUKA)

HSP 1 Score: 76.6 bits (187), Expect = 5.450e-14
Identity = 51/169 (30.18%), Postives = 75/169 (44.38%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATPHTPKPRLIAPLDLSPDAASKAFGALPEILARRILRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAILKLANELG 507
            LF PRG A LY+        R P++S+   G    RF +  T D T +   A +       GG   + + N +L       L++ W T    +P         P A  K      + L R +L    ++   F LQD +W R+S Q+YNT  D + LA A+L+L + LG
Sbjct:   60 LFAPRGCAILYVRKHKQEACRSPIVSNMDEGTFADRFNVQATRDETAFGSAAMAVEVFEKFGGMQGVTKYNTKLVRYGVEKLRNEWGTELLYEPTTFMFAIACPIAEGKIKA---DELMRLLLLYFRIQIAAFSLQDKLWIRVSCQVYNTEDDIDCLASAVLQLKSMLG 225          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A6P8IQ73_ACTTE (putative L-cysteine desulfhydrase 1 n=1 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8IQ73_ACTTE)

HSP 1 Score: 75.1 bits (183), Expect = 1.130e-12
Identity = 58/175 (33.14%), Postives = 88/175 (50.29%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATPHTPKPRLI-APLDLS---PDAASKAFGAL--PEILARR-ILRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAILKLANEL 504
            +F PRG+A L+++PK        V+S       + +F + GT D T +  I  + +F   LGG  A+   N+ LA+ A+  L   W T   P P  + AP   S   P   + A+G     E + RR + +   V T +  +Q ++WCRISA +YNT +DY  LA A  +L + L
Sbjct:  274 MFTPRGSAILWVHPKYQDTINPLVVSWNQDLGFQDKFFMQGTQDQTSYLVIDSASKFYYELGGMNAITGYNKCLASWASLMLATEWKTEVLPIPESMRAPFMASIRLPLEFTAAYGTSCQSEFMIRRDLFQRYRVLTAVCCIQSSLWCRISANVYNTKEDYMKLADAFNRLKHAL 448          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A2V0NQ30_9CHLO (Class V aminotransferase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0NQ30_9CHLO)

HSP 1 Score: 70.5 bits (171), Expect = 4.680e-11
Identity = 60/193 (31.09%), Postives = 80/193 (41.45%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATPHTP--------------KPRLIAPL---------DLSPD---AASKAFGALPE---ILARRILRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAILKL 492
            L  P+G AFL+  P        PV SH      R  F   GT D T W  +      +  LGGA  LA RN  L  +AA  L+ +W     P                RL +PL         D + D   AA +   A PE    L   +     +   +  +Q A+WCR+SAQ YNT + + +LAGA+  L
Sbjct:  308 LCTPKGVAFLWAAPARQTALLPPVTSHGCGLGFRGEFLWQGTGDPTAWLAVPACLEALERLGGAERLAARNHALVWDAAELLRTSWGVEEPPLGAPPGGGACAGMAAVRLPSPLLLPGGAGAGDSAGDGDGAARREVPATPEGAAALQAHLRARHRIEVPLVAVQGALWCRVSAQAYNTLEQFASLAGAVAAL 500          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: R7UPD6_CAPTE (Aminotran_5 domain-containing protein n=1 Tax=Capitella teleta TaxID=283909 RepID=R7UPD6_CAPTE)

HSP 1 Score: 68.2 bits (165), Expect = 2.670e-10
Identity = 59/184 (32.07%), Postives = 84/184 (45.65%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGP-LRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATP--------HTPKPRLIAPLDLSPDAASK--------AFGALPEILARRILRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAILKLANE 501
            +FVPRG A L+++PK     R  V S    GP     F   GT D + + C+ +S  F+  +GG  A+   N +LA  AA  L D   T          +P  R++   D+    A K        A G + E L   I+    ++TV  ++    W R+SAQ+YN  +D   LA AIL L  E
Sbjct:  222 MFVPRGCAVLWVDPKHQKDIRPCVTSSHLDGPHFYKDFLRQGTVDDSPFHCVQESLAFIERIGGMDAIIEHNSKLADQAADLLADLLGTKKFDVPKEMESPFLRMVRLPDMKKYPARKETSDDFTSAVGDIAE-LHNDIMERFKIQTVSLYVHSEPWIRLSAQVYNDLEDIRKLADAILTLKAE 404          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: UPI000FD6BCB5 (aminotransferase class V-fold PLP-dependent enzyme n=1 Tax=Solirhodobacter olei TaxID=2493082 RepID=UPI000FD6BCB5)

HSP 1 Score: 67.4 bits (163), Expect = 4.700e-10
Identity = 54/162 (33.33%), Postives = 76/162 (46.91%), Query Frame = 1
Query:    4 FVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATPHTPKPRLIAPLD--LSPDAASKAFGALPEILARRILRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAI 483
            F P+G   L++ P L        ISH     L+  F  +GT D + W   A++      LGGA A+AR NR LAA A   + +A+ TP +  P + A +   L P  A    GA  E   R I     +   +    + +  RISAQ+YN  +DY AL  A+
Sbjct:  218 FAPKGCGVLWVRPGLGGEVHPTTISHGYGTGLQGEFNWIGTRDASAWFAFAEAVEAHARLGGAEAMAR-NRALAAEAGAMIAEAFGTPISGPPGMRAAMATVLLPLGA----GAESERYHREISARYGMEACLMPWGERLAVRISAQVYNEMEDYEALVAAL 374          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A537BBE9_9PROT (Aminotransferase class V-fold PLP-dependent enzyme n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A537BBE9_9PROT)

HSP 1 Score: 66.2 bits (160), Expect = 1.190e-9
Identity = 51/162 (31.48%), Postives = 72/162 (44.44%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATP-HTPKPRLIAPLDLSPDAASKAFGALPEILARRI----LRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNAL 471
            LF PR  AFL+   +         ISH       + F   GT D + W  + D+ RF+  LG A   A  N  L A AA  + + W TP   P     + + +      +A+G      ARR+    L    V   +  L DA+W RIS Q+YN+++DY  L
Sbjct:  218 LFAPRSCAFLWCRAEAKRALHPLAISHHYGEGFTAEFDWTGTRDFSAWLAVIDALRFLDGLGAARVRAY-NHDLVATAASRIAEFWQTPLDAPAAMHGSMIAMRLPPRLQAYGPPTRDTARRLQSALLAKHRVVVAVMALDDALWARISGQIYNSAEDYERL 378          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A537DMR1_9PROT (Aminotransferase class V-fold PLP-dependent enzyme n=2 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A537DMR1_9PROT)

HSP 1 Score: 66.2 bits (160), Expect = 1.190e-9
Identity = 52/162 (32.10%), Postives = 69/162 (42.59%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATP-HTPKPRLIAPLDLSPDAASKAFGALPEILARRI----LRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNAL 471
            LF PR  AFL+             ISH       + F   GT D + W  + D+ RF   LG A   A  N  L   AA  + +AW TP   P     + + L      +A+G      A R+    L +  V   +  L DA+W RIS Q+YNT +DY  L
Sbjct:  218 LFAPRSCAFLWCREDSKRELHPLAISHHYGEGFTAEFDWTGTRDFSAWLAVVDALRFFDRLGAARVRAY-NHDLVVTAASRIAEAWQTPLDAPAAMHGSMIALRLPPRLQAYGPPTRDTAGRLQSALLAEHRVAVAVMALGDALWARISGQIYNTPEDYEKL 378          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: UPI00168A378C (aminotransferase class V-fold PLP-dependent enzyme n=1 Tax=Oscillatoria sp. FACHB-1407 TaxID=2692847 RepID=UPI00168A378C)

HSP 1 Score: 64.7 bits (156), Expect = 4.260e-9
Identity = 54/185 (29.19%), Postives = 84/185 (45.41%), Query Frame = 1
Query:   10 PRGTAFLYINPKLHAHTRGPVISHTPTGPLRSR------FAIVGTNDCTRWRCIADSFRFVRNL--GGAAALARRNRRLAANAARALQDAWATPHTPKPRLIAPLDLSPDAASKAFGALPEI---------LARRILRDSNVRTVIFHLQDAI--WCRISAQLYNTSQDYNALAGAILKLANELG 507
            P+G  FLY+ P   +  R  VISH    P + R      F  VGT D T + CI  + +F+R+L  GG + L   NR+L  +A   L +A              L ++P   ++  G++  +         L +++  D  +   +   +       RISAQ+YNT  DY+ LA A++KL    G
Sbjct:  230 PKGAGFLYVRPDRQSSIRPLVISHGANSPRQDRSFFHLEFDWVGTTDPTPYLCIPKAIQFLRSLLPGGWSDLMTHNRQLVLSARAVLCEA--------------LGIAPPCPAEMIGSMATLPLPHGNAAALQKKLFHDFGLEVPVIPWKGCSDRLIRISAQIYNTPDDYDCLAAALVKLLTSEG 400          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A1A9T3N2_9PROT (Aminotran_5 domain-containing protein n=1 Tax=Methylovorus sp. MM2 TaxID=1848038 RepID=A0A1A9T3N2_9PROT)

HSP 1 Score: 62.4 bits (150), Expect = 2.650e-8
Identity = 51/182 (28.02%), Postives = 79/182 (43.41%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRCIADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATPHTPKPRLIAPLDLSPDAASKAFGA---LPEILAR------------RILRDSNVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAILKLANE 501
            LF P+G AFL+ +P +  + +  V+S          F   GT D + W  I+ +  F + +GG   + ++    A   A  L DAW              ++   A  + FGA   LP   A+            R+ +   +      + + +W RISAQLYNT  DY  LA A+LKL ++
Sbjct:  222 LFAPKGCAFLWASPAVSENLQPLVVSLRMDEGFPRCFDWTGTRDVSAWLTISAAIDFYQQMGGQN-IPQQLHHFAIEMATMLTDAW--------------NVEIPAGVEQFGAMFTLPVPWAKTITQIEANDWRDRLWKSEQIEVPFLAINERLWVRISAQLYNTKHDYERLAEAVLKLTDQ 388          
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Match: A0A846HJ37_9CYAN (Aminotransferase class V-fold PLP-dependent enzyme n=1 Tax=Hassallia byssoidea VB512170 TaxID=1304833 RepID=A0A846HJ37_9CYAN)

HSP 1 Score: 61.2 bits (147), Expect = 6.730e-8
Identity = 57/185 (30.81%), Postives = 85/185 (45.95%), Query Frame = 1
Query:    1 LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPL--RSRFAI----VGTNDCTRWRCIADSFRFVRNL--GGAAALARRNRRLAANAARALQDAWATPHTPKPRLIAPLDLSPDAASKAFGALPEILARRI---LRDS-------NVRTVIFHLQDAIWCRISAQLYNTSQDYNALAGAILKLANE 501
            L  P+  AFLY+ P      R   ISH    P   +SRF +    +GTND T + C+ ++  F+ +L  GG   L +RN +LA  A + L +A     +    +I          S A   +PE+L  R    LRD         V+ V +  +  +  R+SAQ+YNT + Y  L  A++ L  E
Sbjct:  218 LCAPKSAAFLYVRPDKQPEIRPLTISHGANSPRTDKSRFQLEFDWMGTNDPTAYMCVPEAIAFMGSLLPGGWNELRQRNHQLALQARQLLCEALNVSVSCPDEMI---------GSMAVVPIPEVLESRSFMSLRDELFDRFGIQVQVVPWQEKPKLLIRVSAQIYNTLEQYEYLGKALITLLTE 393          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig72886.17311.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3D6Y8_9EUKA5.450e-1430.18Hypothetical protein n=1 Tax=Palpitomonas bilix Ta... [more]
A0A6P8IQ73_ACTTE1.130e-1233.14putative L-cysteine desulfhydrase 1 n=1 Tax=Actini... [more]
A0A2V0NQ30_9CHLO4.680e-1131.09Class V aminotransferase n=1 Tax=Raphidocelis subc... [more]
R7UPD6_CAPTE2.670e-1032.07Aminotran_5 domain-containing protein n=1 Tax=Capi... [more]
UPI000FD6BCB54.700e-1033.33aminotransferase class V-fold PLP-dependent enzyme... [more]
A0A537BBE9_9PROT1.190e-931.48Aminotransferase class V-fold PLP-dependent enzyme... [more]
A0A537DMR1_9PROT1.190e-932.10Aminotransferase class V-fold PLP-dependent enzyme... [more]
UPI00168A378C4.260e-929.19aminotransferase class V-fold PLP-dependent enzyme... [more]
A0A1A9T3N2_9PROT2.650e-828.02Aminotran_5 domain-containing protein n=1 Tax=Meth... [more]
A0A846HJ37_9CYAN6.730e-830.81Aminotransferase class V-fold PLP-dependent enzyme... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig72886contigM-pyrifera_M_contig72886:1..603 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score73.2
Seed ortholog evalue1.4e-10
Seed eggNOG ortholog10224.XP_006812638.1
KEGG rclassRC00382,RC01784,RC03328
KEGG koko:K20247,ko:K22207
KEGG ReactionR00782,R11023
KEGG Pathwayko00270,ko00340,map00270,map00340
Hectar predicted targeting categorymitochondrion
EggNOG free text desc.Aminotransferase class-V
EggNOG OGs38GRE@33154,3BPFP@33208,3D7Z8@33213,COG1104@1,KOG1549@2759
EC4.4.1.28,4.4.1.36
COG Functional cat.E
Best tax levelBilateria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons2
Model size513
Cds size513
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816626.1161203-CDS-M-pyrifera_M_contig72886:0..3071622816626.1161203-CDS-M-pyrifera_M_contig72886:0..307Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72886 1..307 +
1692278084.857971-CDS-M-pyrifera_M_contig72886:0..3071692278084.857971-CDS-M-pyrifera_M_contig72886:0..307Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72886 1..307 +
1622816626.1316519-CDS-M-pyrifera_M_contig72886:397..6031622816626.1316519-CDS-M-pyrifera_M_contig72886:397..603Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72886 398..603 +
1692278084.8754308-CDS-M-pyrifera_M_contig72886:397..6031692278084.8754308-CDS-M-pyrifera_M_contig72886:397..603Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72886 398..603 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig72886.17311.1prot_M-pyrifera_M_contig72886.17311.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig72886 1..603 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig72886.17311.1

>prot_M-pyrifera_M_contig72886.17311.1 ID=prot_M-pyrifera_M_contig72886.17311.1|Name=mRNA_M-pyrifera_M_contig72886.17311.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=171bp
LFVPRGTAFLYINPKLHAHTRGPVISHTPTGPLRSRFAIVGTNDCTRWRC
IADSFRFVRNLGGAAALARRNRRLAANAARALQDAWATPHTPKPRLIAPL
DLSPDAASKAFGALPEILARRILRDSNVRTVIFHLQDAIWCRISAQLYNT
SQDYNALAGAILKLANELGK*
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mRNA from alignment at M-pyrifera_M_contig72886:1..603+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig72886.17311.1 ID=mRNA_M-pyrifera_M_contig72886.17311.1|Name=mRNA_M-pyrifera_M_contig72886.17311.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=603bp|location=Sequence derived from alignment at M-pyrifera_M_contig72886:1..603+ (Macrocystis pyrifera P11B4 male)
CTCTTTGTGCCACGCGGCACCGCCTTCCTCTACATCAACCCAAAGCTCCA CGCCCACACACGAGGGCCAGTGATCTCACACACCCCGACGGGGCCACTCC GTTCCAGGTTTGCCATCGTCGGAACCAACGACTGCACTCGGTGGAGGTGC ATCGCAGACTCGTTCCGATTCGTGAGGAACCTGGGTGGCGCAGCAGCACT GGCGCGAAGAAACCGTCGCCTCGCTGCCAACGCCGCAAGAGCTCTCCAAG ACGCCTGGGCCACCCCACACACCCCAAAACCCAGGCTCATCGCTCCCCTC GACCTTTGTGGGCCAATGGTAGTGGTGGAGACGCCGCTCTCCGTTGAAAA GTGCACCAAGTACGCCGCAGAGGTCCTCCAATCCACCACACCAGCAGCCC CAGACGCTGCCTCCAAAGCGTTTGGCGCACTCCCAGAAATCCTAGCAAGG CGAATCCTTCGCGATTCGAATGTTCGTACTGTAATATTCCACCTCCAAGA TGCCATTTGGTGCAGAATTTCCGCGCAGCTGTACAACACATCACAAGACT ACAACGCTCTCGCAGGAGCAATCTTGAAACTTGCAAATGAACTTGGAAAA TAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig72886:1..603+

>mRNA_M-pyrifera_M_contig72886.17311.1 ID=mRNA_M-pyrifera_M_contig72886.17311.1|Name=mRNA_M-pyrifera_M_contig72886.17311.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1026bp|location=Sequence derived from alignment at M-pyrifera_M_contig72886:1..603+ (Macrocystis pyrifera P11B4 male)
CTCTTTGTGCCACGCGGCACCGCCTTCCTCTACATCAACCCAAAGCTCCA
CGCCCACACACGAGGGCCAGTGATCTCACACACCCCGACGGGGCCACTCC
GTTCCAGGTTTGCCATCGTCGGAACCAACGACTGCACTCGGTGGAGGTGC
ATCGCAGACTCGTTCCGATTCGTGAGGAACCTGGGTGGCGCAGCAGCACT
GGCGCGAAGAAACCGTCGCCTCGCTGCCAACGCCGCAAGAGCTCTCCAAG
ACGCCTGGGCCACCCCACACACCCCAAAACCCAGGCTCATCGCTCCCCTC
GACCTTTCTCTTTGTGCCACGCGGCACCGCCTTCCTCTACATCAACCCAA
AGCTCCACGCCCACACACGAGGGCCAGTGATCTCACACACCCCGACGGGG
CCACTCCGTTCCAGGTTTGCCATCGTCGGAACCAACGACTGCACTCGGTG
GAGGTGCATCGCAGACTCGTTCCGATTCGTGAGGAACCTGGGTGGCGCAG
CAGCACTGGCGCGAAGAAACCGTCGCCTCGCTGCCAACGCCGCAAGAGCT
CTCCAAGACGCCTGGGCCACCCCACACACCCCAAAACCCAGGCTCATCGC
TCCCCTCGACCTTTCCCCAGACGCTGCCTCCAAAGCGTTTGGCGCACTCC
CAGAAATCCTAGCAAGGCGAATCCTTCGCGATTCGAATGTTCGTACTGTA
ATATTCCACCTCCAAGATGCCATTTGGTGCAGAATTTCCGCGCAGCTGTA
CAACACATCACAAGACTACAACGCTCTCGCAGGAGCAATCTTGAAACTTG
CAAATGAACTTGGAAAATAGCCCCAGACGCTGCCTCCAAAGCGTTTGGCG
CACTCCCAGAAATCCTAGCAAGGCGAATCCTTCGCGATTCGAATGTTCGT
ACTGTAATATTCCACCTCCAAGATGCCATTTGGTGCAGAATTTCCGCGCA
GCTGTACAACACATCACAAGACTACAACGCTCTCGCAGGAGCAATCTTGA
AACTTGCAAATGAACTTGGAAAATAG
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