mRNA_M-pyrifera_M_contig72835.17299.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig72835.17299.1
Unique NamemRNA_M-pyrifera_M_contig72835.17299.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A3D5DAX7_9GAMM (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DAX7_9GAMM)

HSP 1 Score: 244 bits (623), Expect = 3.510e-79
Identity = 114/153 (74.51%), Postives = 131/153 (85.62%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTGTIV SNFLDPE  F+S+VTANNLYHDW IT NLGRVVRE AD L RR+AIVG+GGLSG+FFRHVI+I +DHIA+ A+D WN+RIL LME GDV+ L  ACP +A +ARAEMGFKHFAY+LGGI G++ GA VHHYGPLYG+GGAVVEF L
Sbjct:  118 DTGTIVGSNFLDPERNFQSVVTANNLYHDWAITENLGRVVREQADTLGRRIAIVGIGGLSGSFFRHVINIGEDHIANTAEDEWNKRILGLMEVGDVEALVQACPTYAAQARAEMGFKHFAYILGGISGKYTGATVHHYGPLYGSGGAVVEFQL 270          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A523PFQ7_9PROT (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=A0A523PFQ7_9PROT)

HSP 1 Score: 231 bits (590), Expect = 3.470e-74
Identity = 110/153 (71.90%), Postives = 129/153 (84.31%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTGTIVA NFLDP+  + S++TANN+YHDW +T NLGRVVRE A KL RRVAIVGVG LSG++FRH I+I+DDHIAS  +DAWN+RIL +ME GD  GL  ACP+FA +ARAEMGFKHFA++LGG+GG F  A VHHYGPLYG+GGAVVEFHL
Sbjct:  118 DTGTIVALNFLDPQASYLSVLTANNVYHDWGLTENLGRVVREQAAKLGRRVAIVGVGCLSGSYFRHSIEISDDHIASAEEDAWNQRILRMMERGDSAGLVDACPEFATQARAEMGFKHFAFILGGVGGGFEAATVHHYGPLYGSGGAVVEFHL 270          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A6L8ENA2_9GAMM (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A6L8ENA2_9GAMM)

HSP 1 Score: 204 bits (520), Expect = 1.460e-63
Identity = 92/153 (60.13%), Postives = 121/153 (79.08%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTG IVASNFL+P+      +T+NN+YHDW +T +LGRV  ++A +L R++A++GVGGLSG+ +RHVI+I++D IA  A+D WNR+IL++ME GD + L  ACPD+A  AR +MGFKHFA++LG IGG F GA VH YGPLYG+GGAVVEF L
Sbjct:  118 DTGAIVASNFLNPDGNIPLAITSNNVYHDWQMTESLGRVAADSASELGRKLAVIGVGGLSGSIYRHVINISEDKIADSAEDDWNRKILSMMEQGDTEALAKACPDYAGAARVDMGFKHFAFILGAIGGNFNGATVHAYGPLYGSGGAVVEFDL 270          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A2N5WXP2_9GAMM (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Pseudohalioglobus lutimaris TaxID=1737061 RepID=A0A2N5WXP2_9GAMM)

HSP 1 Score: 202 bits (514), Expect = 1.150e-62
Identity = 94/151 (62.25%), Postives = 120/151 (79.47%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEF 453
            DTGTIVAS  L+P N+    +T+NN+YHDW +T +LGR+  + A  L RR+A+VGVGGLSG++FRH IDIA+D IASE++D WNR+IL+LME GD+  L   CP +A EAR +MGFKHFA++LG +GG F+ A+VH YGPLYGAGGAVVEF
Sbjct:  118 DTGTIVASTMLNPANKLPLAITSNNVYHDWSMTESLGRIAADQAATLGRRIAVVGVGGLSGSYFRHDIDIAEDRIASESEDQWNRKILSLMEQGDLAALREQCPTYAAEARVDMGFKHFAFLLGAMGGTFSCAVVHGYGPLYGAGGAVVEF 268          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A1G0K863_9GAMM (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Gammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_61_13 TaxID=1798300 RepID=A0A1G0K863_9GAMM)

HSP 1 Score: 197 bits (502), Expect = 7.520e-61
Identity = 92/153 (60.13%), Postives = 116/153 (75.82%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTGTIVA+++L+P +    ++ ANN+YHDW  T +LG +    A  L RR+A+VGVGG+SG+FFRH ID   DHIA  A+D WNRRIL LME GD+  L+  CP +AREAR +MGFKHFA+VLG +GGRF+GA VH YGPLYG+G AVVEF +
Sbjct:  115 DTGTIVANHYLNPGSTRPLVIAANNVYHDWQRTMDLGAIANRCAATLGRRIAVVGVGGMSGSFFRHAIDEKKDHIAIAAEDRWNRRILGLMEKGDLPALSKECPRYAREARVDMGFKHFAFVLGALGGRFSGATVHGYGPLYGSGAAVVEFRI 267          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A2E7QG70_9PROT (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2E7QG70_9PROT)

HSP 1 Score: 174 bits (441), Expect = 1.250e-51
Identity = 83/153 (54.25%), Postives = 107/153 (69.93%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTGTIVA+  ++P      ++ ANNLYH W+ T  LG V    ADKL RRVA++GVG LSG  FR  IDI +D IAS  DD WN+R+L L+E+GDV       P++A+EAR +MGFKHF ++LG +GGRF GA +H YG +YG+G AVVEF +
Sbjct:  118 DTGTIVANALMNPRGSIPLVIAANNLYHSWEETYQLGEVAAREADKLGRRVAVLGVGSLSGTIFRDDIDIREDGIASTKDDEWNKRMLKLIESGDVTQARDLTPEYAQEARVDMGFKHFGWILGALGGRFHGARIHAYGAVYGSGAAVVEFKI 270          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A6B1HT47_9PROT (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=4 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A6B1HT47_9PROT)

HSP 1 Score: 172 bits (436), Expect = 7.080e-51
Identity = 85/153 (55.56%), Postives = 106/153 (69.28%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            D+GTIVA+  LDP+ RF   + ANNLYHD   T  +  +    A   +++VA+VGVGGLSG  FR  I I  D IA+E+DDAWNRRIL L+E G+   L +   D+AREAR +MGFKHFA+V G +GGRF GA VH YGP +GAG AV+EF L
Sbjct:  118 DSGTIVATRLLDPDGRFPLAMAANNLYHDAATTMKMAGIAAATARAQNKKVAVVGVGGLSGTVFREPIAIEQDRIAAESDDAWNRRILRLLEKGEAGQLDSRIEDYAREARVDMGFKHFAWVRGAVGGRFFGARVHGYGPAHGAGAAVIEFRL 270          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A0P6RNT5_9RHOB (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Phaeobacter sp. 11ANDIMAR09 TaxID=1225647 RepID=A0A0P6RNT5_9RHOB)

HSP 1 Score: 169 bits (429), Expect = 8.050e-50
Identity = 83/153 (54.25%), Postives = 106/153 (69.28%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTGTIVA  FL+P +R+   + ANNLYH +D T  LG +  + A  L RRVAIVGVGGLSG+ FRH IDI  D I S  DDAWNR++L+ +EAG        C +FA+ A+A+MGFKH A++ G +G +F+GA VH YG  YG+G AVVEF +
Sbjct:  118 DTGTIVADKFLNPSSRYPLTIAANNLYHSFDDTIRLGEMAVQKATDLGRRVAIVGVGGLSGSIFRHEIDIRSDGIVSAEDDAWNRQMLSSLEAGGDASFPEVCDEFAKGAKADMGFKHCAWIYGALGRKFSGARVHEYGATYGSGAAVVEFKV 270          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: UPI0007E3B81B (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein n=1 Tax=Cupriavidus gilardii TaxID=82541 RepID=UPI0007E3B81B)

HSP 1 Score: 162 bits (410), Expect = 5.880e-47
Identity = 83/154 (53.90%), Postives = 103/154 (66.88%), Query Frame = 1
Query:    1 DTGTIVASNFLD-PENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            DTGTI A+  +         +V ANNLYH  + T  LG +   AA   D+RVA+VG+GGLS + FR  ID+  DHI S+ADD WNRR+L LME GD+D L AA P +A EAR +MG KHF ++LG +GG F  A VH Y PLYG+GGAVVEF +
Sbjct:  118 DTGTITATTLMSIGSTELPVVVAANNLYHGAEQTEQLGAIASAAAR--DKRVAVVGIGGLSSSMFRENIDMETDHIVSDADDKWNRRVLGLMEGGDIDALRAALPQYAAEARPDMGLKHFYWLLGAMGGSFKKATVHQYAPLYGSGGAVVEFSV 269          
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Match: A0A1D2ULX0_9BURK (tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein (Fragment) n=1 Tax=Lautropia sp. SCN 66-9 TaxID=1660106 RepID=A0A1D2ULX0_9BURK)

HSP 1 Score: 159 bits (402), Expect = 8.900e-47
Identity = 83/154 (53.90%), Postives = 105/154 (68.18%), Query Frame = 1
Query:    1 DTGTIVASNFLDPENRFKSLVTA-NNLYHDWDITTNLGRVVREAADKLDRRVAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLTAACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVEFHL 459
            D+GTI AS+ L   +  + LV A NNLYHD   T  LG +    AD+  +RVA+VGVGGLSG+ FR  ID   D +AS  DD WN+R+L L+E+GDV  L    P+F+  ARA+MGFKHF ++LG + GRF GA VH YGP+YG+G AVVEF L
Sbjct:   35 DSGTIAASSLLGIGSAERPLVVASNNLYHDAQTTEQLGALAAACADEQGKRVAVVGVGGLSGSLFRTEIDPRSDRLASSDDDKWNQRVLRLIESGDVAELRRVIPEFSSGARADMGFKHFHWILGALKGRFVGARVHAYGPVYGSGAAVVEFTL 188          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig72835.17299.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D5DAX7_9GAMM3.510e-7974.51tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A523PFQ7_9PROT3.470e-7471.90tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A6L8ENA2_9GAMM1.460e-6360.13tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A2N5WXP2_9GAMM1.150e-6262.25tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A1G0K863_9GAMM7.520e-6160.13tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A2E7QG70_9PROT1.250e-5154.25tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A6B1HT47_9PROT7.080e-5155.56tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A0P6RNT5_9RHOB8.050e-5054.25tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
UPI0007E3B81B5.880e-4753.90tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]
A0A1D2ULX0_9BURK8.900e-4753.90tRNA U-34 5-methylaminomethyl-2-thiouridine biosyn... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig72835contigM-pyrifera_M_contig72835:654..1115 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score157.5
Seed ortholog evalue4.9e-36
Seed eggNOG ortholog390235.PputW619_2013
Preferred nameamnA
KEGG rclassRC00387,RC00643
KEGG koko:K00455,ko:K15058,ko:K15059
KEGG ReactionR03303,R05405
KEGG Pathwayko00350,ko00627,ko01120,ko01220,map00350,map00627,map01120,map01220
KEGG ModuleM00533
Hectar predicted targeting categoryother localisation
EggNOG free text desc.3-carboxyethylcatechol 2,3-dioxygenase activity
EggNOG OGsCOG3384@1,COG3384@2
EC1.13.11.15,1.13.11.74,1.13.11.76
COG Functional cat.S
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size462
Cds size462
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816625.615809-CDS-M-pyrifera_M_contig72835:653..11151622816625.615809-CDS-M-pyrifera_M_contig72835:653..1115Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72835 654..1115 -
1692278084.6290023-CDS-M-pyrifera_M_contig72835:653..11151692278084.6290023-CDS-M-pyrifera_M_contig72835:653..1115Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72835 654..1115 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig72835.17299.1prot_M-pyrifera_M_contig72835.17299.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig72835 654..1115 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig72835.17299.1

>prot_M-pyrifera_M_contig72835.17299.1 ID=prot_M-pyrifera_M_contig72835.17299.1|Name=mRNA_M-pyrifera_M_contig72835.17299.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=154bp
DTGTIVASNFLDPENRFKSLVTANNLYHDWDITTNLGRVVREAADKLDRR
VAIVGVGGLSGAFFRHVIDIADDHIASEADDAWNRRILALMEAGDVDGLT
AACPDFAREARAEMGFKHFAYVLGGIGGRFAGAMVHHYGPLYGAGGAVVE
FHL*
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mRNA from alignment at M-pyrifera_M_contig72835:654..1115-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig72835.17299.1 ID=mRNA_M-pyrifera_M_contig72835.17299.1|Name=mRNA_M-pyrifera_M_contig72835.17299.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=462bp|location=Sequence derived from alignment at M-pyrifera_M_contig72835:654..1115- (Macrocystis pyrifera P11B4 male)
GATACCGGCACGATCGTCGCGTCGAATTTTCTCGATCCGGAAAACCGCTT CAAGTCACTCGTCACCGCGAACAACCTGTATCACGACTGGGACATCACGA CGAACCTCGGTCGGGTCGTGCGCGAGGCGGCCGACAAGCTCGACCGACGC GTCGCGATCGTCGGGGTGGGCGGACTTTCCGGTGCGTTTTTCCGTCACGT CATCGATATCGCGGACGACCATATCGCCAGCGAAGCGGACGATGCGTGGA ATCGCCGCATCCTCGCGCTGATGGAAGCCGGCGACGTCGACGGTCTCACC GCGGCGTGCCCGGATTTCGCGCGCGAGGCGCGGGCGGAGATGGGGTTCAA GCATTTCGCCTATGTGCTCGGCGGGATCGGCGGCCGGTTCGCGGGCGCCA TGGTGCATCATTACGGTCCGCTGTACGGCGCCGGCGGCGCCGTGGTCGAG TTTCACCTGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig72835:654..1115-

>mRNA_M-pyrifera_M_contig72835.17299.1 ID=mRNA_M-pyrifera_M_contig72835.17299.1|Name=mRNA_M-pyrifera_M_contig72835.17299.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=924bp|location=Sequence derived from alignment at M-pyrifera_M_contig72835:654..1115- (Macrocystis pyrifera P11B4 male)
GATACCGGCACGATCGTCGCGTCGAATTTTCTCGATCCGGAAAACCGCTT
CAAGTCACTCGTCACCGCGAACAACCTGTATCACGACTGGGACATCACGA
CGAACCTCGGTCGGGTCGTGCGCGAGGCGGCCGACAAGCTCGACCGACGC
GTCGCGATCGTCGGGGTGGGCGGACTTTCCGGTGCGTTTTTCCGTCACGT
CATCGATATCGCGGACGACCATATCGCCAGCGAAGCGGACGATGCGTGGA
ATCGCCGCATCCTCGCGCTGATGGAAGCCGGCGACGTCGACGGTCTCACC
GCGGCGTGCCCGGATTTCGCGCGCGAGGCGCGGGCGGAGATGGGGTTCAA
GCATTTCGCCTATGTGCTCGGCGGGATCGGCGGCCGGTTCGCGGGCGCCA
TGGTGCATCATTACGGTCCGCTGTACGGCGCCGGCGGCGCCGTGGTCGAG
TTTCACCTGTAAGATACCGGCACGATCGTCGCGTCGAATTTTCTCGATCC
GGAAAACCGCTTCAAGTCACTCGTCACCGCGAACAACCTGTATCACGACT
GGGACATCACGACGAACCTCGGTCGGGTCGTGCGCGAGGCGGCCGACAAG
CTCGACCGACGCGTCGCGATCGTCGGGGTGGGCGGACTTTCCGGTGCGTT
TTTCCGTCACGTCATCGATATCGCGGACGACCATATCGCCAGCGAAGCGG
ACGATGCGTGGAATCGCCGCATCCTCGCGCTGATGGAAGCCGGCGACGTC
GACGGTCTCACCGCGGCGTGCCCGGATTTCGCGCGCGAGGCGCGGGCGGA
GATGGGGTTCAAGCATTTCGCCTATGTGCTCGGCGGGATCGGCGGCCGGT
TCGCGGGCGCCATGGTGCATCATTACGGTCCGCTGTACGGCGCCGGCGGC
GCCGTGGTCGAGTTTCACCTGTAA
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