mRNA_M-pyrifera_M_contig72710.17272.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig72710.17272.1
Unique NamemRNA_M-pyrifera_M_contig72710.17272.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: UPI0007EFA745 (probable phospholipid hydroperoxide glutathione peroxidase n=1 Tax=Daucus carota subsp. sativus TaxID=79200 RepID=UPI0007EFA745)

HSP 1 Score: 170 bits (430), Expect = 6.630e-50
Identity = 92/178 (51.69%), Postives = 119/178 (66.85%), Query Frame = 1
Query:   37 GASGSTAGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLLNDA 570
            G+S +TA D KP S+ +F+  D  G     S+++G +  LIVNVASQCGLT +NY+ELA L   YKD+G   + FPCNQF SQE G++EEI  F  +K      IF KVKVNG DA+PL+++LK +K      +FG  +KWNF KFLVD  GNVV+ Y PTT+P +I  DI+KLL  A
Sbjct:   58 GSSMATASD-KPKSVHDFTVKDAKGNDVNLSQYKG-KVLLIVNVASQCGLTNSNYTELAKLYEKYKDQGLEILAFPCNQFGSQEPGTNEEIVEFACTKFKAEYPIFDKVKVNGSDANPLYKYLKSSKGG----IFGDGIKWNFTKFLVDENGNVVDRYAPTTSPLSIEKDIKKLLEKA 229          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A078HL84_BRANA (Glutathione peroxidase n=10 Tax=Brassicaceae TaxID=3700 RepID=A0A078HL84_BRANA)

HSP 1 Score: 165 bits (417), Expect = 6.090e-48
Identity = 86/171 (50.29%), Postives = 114/171 (66.67%), Query Frame = 1
Query:   49 STAGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLL 561
            S A  S+P S+++F+  D  G     S ++G +  LIVNVASQCGLT +NY+ELA L + YKD GF  + FPCNQF +QE GS+EEI  F  ++      IF KV VNG  A P++++LK +K      LFG  +KWNFAKFLVD +GNVV+ Y PTT+P +I  D++KLL
Sbjct:   60 SMAASSEPKSIYDFTVKDAKGNDVDLSTYKG-KVLLIVNVASQCGLTNSNYTELAQLYQKYKDHGFEILAFPCNQFGNQEPGSNEEIVQFACTRFKAEYPIFDKVDVNGDSAAPIYKFLKSSKGG----LFGDGIKWNFAKFLVDKDGNVVDRYAPTTSPLSIEKDLKKLL 225          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A6J0LW45_RAPSA (Glutathione peroxidase n=3 Tax=Brassicaceae TaxID=3700 RepID=A0A6J0LW45_RAPSA)

HSP 1 Score: 162 bits (410), Expect = 1.180e-47
Identity = 85/169 (50.30%), Postives = 113/169 (66.86%), Query Frame = 1
Query:   55 AGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLL 561
            A  S P S+ +F+  D  G     S ++G +A LIVNVASQCGLT +NY+ELA L + YKD GF  + FPCNQF +QE GS+EEI  F  ++      IF KV VNG  A P++++LK +K      LFG+ +KWNF+KFLVD +GNVV+ Y PTT+P +I  D++KLL
Sbjct:    2 AATSAPKSVHDFTVKDAKGNEVDLSSYKG-KALLIVNVASQCGLTNSNYTELAQLYQKYKDHGFEILAFPCNQFGNQEPGSNEEIVQFACTRFKAEYPIFDKVDVNGDKAAPIYKFLKSSKGG----LFGSGIKWNFSKFLVDKDGNVVDRYAPTTSPLSIEKDLKKLL 165          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A087GPW6_ARAAL (Glutathione peroxidase n=1 Tax=Arabis alpina TaxID=50452 RepID=A0A087GPW6_ARAAL)

HSP 1 Score: 164 bits (414), Expect = 1.780e-47
Identity = 85/169 (50.30%), Postives = 113/169 (66.86%), Query Frame = 1
Query:   55 AGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLL 561
            A  S P S+++F+  D  G     S ++G +  LIVNVASQCGLT +NY+ELA L + YKD GF  + FPCNQF +QE GS+EEI AF  ++      IF KV VNG  A P++++L+ +K      LFG  +KWNFAKFL+D EGNVV+ Y PTT+P +I  D++KLL
Sbjct:   63 ATSSAPKSIYDFTVKDAKGSDVDLSTYKG-KVLLIVNVASQCGLTNSNYTELAQLYQKYKDHGFEILAFPCNQFGNQEPGSNEEIVAFACTRFKAEYPIFDKVDVNGDKAAPIYKFLRSSKGG----LFGDGIKWNFAKFLIDKEGNVVDRYAPTTSPLSIEKDLKKLL 226          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A2C9WIS4_MANES (Glutathione peroxidase n=2 Tax=Malpighiales TaxID=3646 RepID=A0A2C9WIS4_MANES)

HSP 1 Score: 161 bits (407), Expect = 3.150e-47
Identity = 83/172 (48.26%), Postives = 114/172 (66.28%), Query Frame = 1
Query:   55 AGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLLNDA 570
            A  S+P S+ +F+  D  G     S ++G +  LIVNVASQCGLT +NY+EL  L + YKD+GF  + FPCNQF SQE G++EEI  F  ++      IF K+ VNG +A PL+++LK +K      +FG  +KWNF+KFLVD +GNVV+ Y PTT+P +I  D++KLL  A
Sbjct:    2 ASQSQPKSVHDFAVKDARGNDVDLSIYKG-KVLLIVNVASQCGLTNSNYTELTQLYQKYKDQGFEILAFPCNQFGSQEPGTNEEIVEFACTRFKAEYPIFDKIDVNGNNAAPLYKFLKSSKGG----IFGDSIKWNFSKFLVDKDGNVVDRYAPTTSPLSIEKDVKKLLGIA 168          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: GPX4_HELAN (Probable phospholipid hydroperoxide glutathione peroxidase n=2 Tax=Helianthus annuus TaxID=4232 RepID=GPX4_HELAN)

HSP 1 Score: 161 bits (408), Expect = 3.210e-47
Identity = 85/161 (52.80%), Postives = 110/161 (68.32%), Query Frame = 1
Query:   88 FSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLLNDA 570
            FS  D+ G+    S+++G +  LIVNVASQCG T +NY EL  L + YKD+GF  + FPCNQF  QE GS+EEI+ F  ++      +FSKV VNGK+A PL+++LK +K   G FL G  +KWNF KFLVD EG VV+ Y PTT+P +I  DI+KLLN A
Sbjct:   25 FSDKDVKGQDVELSKYKG-KVLLIVNVASQCGFTNSNYPELTTLYQKYKDQGFEILAFPCNQFGGQEPGSNEEIQVFACTRFKAEYPVFSKVNVNGKEADPLYKFLKSSK---GGFL-GDSIKWNFTKFLVDREGKVVDRYAPTTSPLSIEKDIKKLLNVA 180          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A830BGF6_9LAMI (Glutathione peroxidase n=1 Tax=Phtheirospermum japonicum TaxID=374723 RepID=A0A830BGF6_9LAMI)

HSP 1 Score: 162 bits (409), Expect = 3.800e-47
Identity = 86/169 (50.89%), Postives = 112/169 (66.27%), Query Frame = 1
Query:   55 AGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLL 561
            A +S P S+++F+  DI G   P S +RG +  LIVNVAS+CGLT++NY EL  L   YKD+GF  + FPCNQF SQE G++EEIE  + ++      IF KV+VNGK+  PL+++LK  K      LF   +KWNF KFLVD +G VVE Y PTT+P AI  D+E LL
Sbjct:   32 ASESSPKSIYDFTVKDIRGNDVPLSNYRG-KVLLIVNVASKCGLTQSNYKELNILYEKYKDQGFEILAFPCNQFGSQEPGTNEEIEEGVCTRLKVEFPIFDKVEVNGKNTVPLYKFLKSEKGG----LFVDAIKWNFTKFLVDKKGKVVERYAPTTSPLAIEKDVEYLL 195          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A2I4H8J7_JUGRE (Glutathione peroxidase n=3 Tax=Juglans TaxID=16718 RepID=A0A2I4H8J7_JUGRE)

HSP 1 Score: 163 bits (412), Expect = 4.490e-47
Identity = 85/172 (49.42%), Postives = 117/172 (68.02%), Query Frame = 1
Query:   55 AGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLLNDA 570
            AG S+ S++ +F+  D  G     S+++G +  LIVNVASQCGLT +NY+EL+ L   YKD+G   + FPCNQF +QE G++E+I  F  ++      IF KV VNGKDA PL+++LK +K      LFG ++KWNF+KFLVD +GNVV+ Y PTT+P +I  DI+KLL  A
Sbjct:   72 AGQSQNSTVHDFTVKDARGNDVDLSQYKG-KTLLIVNVASQCGLTNSNYTELSQLYGKYKDQGLEILAFPCNQFGAQEPGNNEQILEFACTRFKAEYPIFDKVDVNGKDAAPLYKFLKSSKGG----LFGDNIKWNFSKFLVDKDGNVVDRYAPTTSPLSIEKDIKKLLGSA 238          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: R4USJ3_9APIA (Glutathione peroxidase (Fragment) n=1 Tax=Eleutherococcus senticosus TaxID=82096 RepID=R4USJ3_9APIA)

HSP 1 Score: 160 bits (406), Expect = 4.600e-47
Identity = 84/172 (48.84%), Postives = 114/172 (66.28%), Query Frame = 1
Query:   55 AGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLLNDA 570
            A  SKP S+ +F+  D  G     S+++G +  LIVNVASQCGLT +NY+ELA +   YK++G   + FPCNQF SQE G++EEI  F  ++      IF K+ VNG +A PL+++LK +K      LFG  +KWNF+KFLVD +GNVV+ Y PTT+P +I  DI+KLL  A
Sbjct:    3 AQSSKPKSVHDFTVKDARGNDVDLSQYKG-KVLLIVNVASQCGLTNSNYTELAKVYEKYKNQGLEILAFPCNQFGSQEPGTNEEIVEFACTRFKAEYPIFDKIDVNGSNAAPLYKYLKSSKGG----LFGDGIKWNFSKFLVDKDGNVVDRYAPTTSPLSIEKDIKKLLEKA 169          
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Match: A0A7C8ZHU1_OPUST (Glutathione peroxidase n=1 Tax=Opuntia streptacantha TaxID=393608 RepID=A0A7C8ZHU1_OPUST)

HSP 1 Score: 161 bits (407), Expect = 5.640e-47
Identity = 87/181 (48.07%), Postives = 113/181 (62.43%), Query Frame = 1
Query:   19 FHFADTGASGSTAGDSKPSSLFEFSADDIHGKPTPFSRFRGYRAFLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSHEEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGADLKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLL 561
            F F     S ++   ++P S+ EF   D  G     S ++G +  LIVNVASQCGLT +NY+EL  L   YKD+G   + FPCNQF +QE G++EEI  F  ++      IF KV VNGKDA PL+++LK  K      +FG  +KWNF KFLVD +G VVE Y PTT+PS+I  DI+KLL
Sbjct:    9 FQFLRFEQSMASQSAAEPKSVHEFVVKDARGNDVDLSTYKG-KVLLIVNVASQCGLTNSNYTELTQLYENYKDKGLEILAFPCNQFGNQEPGNNEEIMQFACTRFKAEFPIFDKVDVNGKDAAPLYKYLKRAKGG----IFGDGIKWNFTKFLVDQDGKVVERYAPTTSPSSIEKDIKKLL 184          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig72710.17272.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI0007EFA7456.630e-5051.69probable phospholipid hydroperoxide glutathione pe... [more]
A0A078HL84_BRANA6.090e-4850.29Glutathione peroxidase n=10 Tax=Brassicaceae TaxID... [more]
A0A6J0LW45_RAPSA1.180e-4750.30Glutathione peroxidase n=3 Tax=Brassicaceae TaxID=... [more]
A0A087GPW6_ARAAL1.780e-4750.30Glutathione peroxidase n=1 Tax=Arabis alpina TaxID... [more]
A0A2C9WIS4_MANES3.150e-4748.26Glutathione peroxidase n=2 Tax=Malpighiales TaxID=... [more]
GPX4_HELAN3.210e-4752.80Probable phospholipid hydroperoxide glutathione pe... [more]
A0A830BGF6_9LAMI3.800e-4750.89Glutathione peroxidase n=1 Tax=Phtheirospermum jap... [more]
A0A2I4H8J7_JUGRE4.490e-4749.42Glutathione peroxidase n=3 Tax=Juglans TaxID=16718... [more]
R4USJ3_9APIA4.600e-4748.84Glutathione peroxidase (Fragment) n=1 Tax=Eleuther... [more]
A0A7C8ZHU1_OPUST5.640e-4748.07Glutathione peroxidase n=1 Tax=Opuntia streptacant... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig72710contigM-pyrifera_M_contig72710:373..942 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score167.2
Seed ortholog evalue7.6e-39
Seed eggNOG ortholog3711.Bra033140.1-P
KEGG rclassRC00011,RC00982
KEGG koko:K00432
KEGG ReactionR00274,R07034,R07035
KEGG Pathwayko00480,ko00590,ko04918,map00480,map00590,map04918
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004601,GO:0004602,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005829,GO:0005886,GO:0006950,GO:0006970,GO:0008150,GO:0008152,GO:0009507,GO:0009536,GO:0009628,GO:0009636,GO:0009651,GO:0009987,GO:0010035,GO:0010038,GO:0016020,GO:0016209,GO:0016491,GO:0016684,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044424,GO:0044444,GO:0044464,GO:0046686,GO:0048046,GO:0050896,GO:0051716,GO:0055114,GO:0070887,GO:0071944,GO:0097237,GO:0098754,GO:0098869,GO:1990748
EggNOG free text desc.Belongs to the glutathione peroxidase family
EggNOG OGs37IT1@33090,3GE5K@35493,3HSAT@3699,COG0386@1,KOG1651@2759
EC1.11.1.9
COG Functional cat.O
Best tax levelBrassicales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size570
Cds size570
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816624.1150186-CDS-M-pyrifera_M_contig72710:372..9421622816624.1150186-CDS-M-pyrifera_M_contig72710:372..942Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72710 373..942 +
1692278083.6661987-CDS-M-pyrifera_M_contig72710:372..9421692278083.6661987-CDS-M-pyrifera_M_contig72710:372..942Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72710 373..942 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig72710.17272.1prot_M-pyrifera_M_contig72710.17272.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig72710 373..942 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig72710.17272.1

>prot_M-pyrifera_M_contig72710.17272.1 ID=prot_M-pyrifera_M_contig72710.17272.1|Name=mRNA_M-pyrifera_M_contig72710.17272.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=190bp
YVIHLAFHFADTGASGSTAGDSKPSSLFEFSADDIHGKPTPFSRFRGYRA
FLIVNVASQCGLTETNYSELADLERLYKDRGFLPMLFPCNQFKSQESGSH
EEIEAFIKSKGLENVAIFSKVKVNGKDAHPLFQWLKETKPSFGSFLFGAD
LKWNFAKFLVDHEGNVVESYLPTTAPSAIVPDIEKLLNDA
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mRNA from alignment at M-pyrifera_M_contig72710:373..942+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig72710.17272.1 ID=mRNA_M-pyrifera_M_contig72710.17272.1|Name=mRNA_M-pyrifera_M_contig72710.17272.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig72710:373..942+ (Macrocystis pyrifera P11B4 male)
TACGTCATTCATCTGGCTTTTCATTTCGCCGATACAGGTGCATCTGGAAG TACTGCTGGAGATTCCAAACCATCATCATTGTTTGAATTCAGTGCTGATG ACATTCATGGAAAACCAACCCCATTTTCGAGATTTCGTGGCTATCGCGCT TTTTTGATTGTCAATGTTGCCAGTCAGTGCGGTTTGACTGAAACAAACTA CAGCGAACTTGCAGATTTGGAAAGGTTGTACAAGGACAGAGGGTTTCTCC CCATGCTCTTCCCATGCAACCAATTCAAGAGCCAAGAAAGCGGTTCGCAT GAAGAAATTGAGGCATTCATCAAGAGCAAGGGCCTTGAGAACGTCGCGAT CTTTTCCAAGGTGAAAGTCAACGGTAAAGATGCACATCCTCTCTTCCAGT GGCTGAAGGAAACCAAGCCGTCGTTTGGCTCCTTCCTCTTTGGAGCGGAT CTCAAGTGGAACTTTGCAAAGTTCCTCGTTGACCATGAAGGGAACGTGGT GGAGTCGTACCTCCCAACCACAGCACCATCCGCAATCGTACCAGACATTG AGAAGCTCCTCAACGATGCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig72710:373..942+

>mRNA_M-pyrifera_M_contig72710.17272.1 ID=mRNA_M-pyrifera_M_contig72710.17272.1|Name=mRNA_M-pyrifera_M_contig72710.17272.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1140bp|location=Sequence derived from alignment at M-pyrifera_M_contig72710:373..942+ (Macrocystis pyrifera P11B4 male)
TACGTCATTCATCTGGCTTTTCATTTCGCCGATACAGGTGCATCTGGAAG
TACTGCTGGAGATTCCAAACCATCATCATTGTTTGAATTCAGTGCTGATG
ACATTCATGGAAAACCAACCCCATTTTCGAGATTTCGTGGCTATCGCGCT
TTTTTGATTGTCAATGTTGCCAGTCAGTGCGGTTTGACTGAAACAAACTA
CAGCGAACTTGCAGATTTGGAAAGGTTGTACAAGGACAGAGGGTTTCTCC
CCATGCTCTTCCCATGCAACCAATTCAAGAGCCAAGAAAGCGGTTCGCAT
GAAGAAATTGAGGCATTCATCAAGAGCAAGGGCCTTGAGAACGTCGCGAT
CTTTTCCAAGGTGAAAGTCAACGGTAAAGATGCACATCCTCTCTTCCAGT
GGCTGAAGGAAACCAAGCCGTCGTTTGGCTCCTTCCTCTTTGGAGCGGAT
CTCAAGTGGAACTTTGCAAAGTTCCTCGTTGACCATGAAGGGAACGTGGT
GGAGTCGTACCTCCCAACCACAGCACCATCCGCAATCGTACCAGACATTG
AGAAGCTCCTCAACGATGCCTACGTCATTCATCTGGCTTTTCATTTCGCC
GATACAGGTGCATCTGGAAGTACTGCTGGAGATTCCAAACCATCATCATT
GTTTGAATTCAGTGCTGATGACATTCATGGAAAACCAACCCCATTTTCGA
GATTTCGTGGCTATCGCGCTTTTTTGATTGTCAATGTTGCCAGTCAGTGC
GGTTTGACTGAAACAAACTACAGCGAACTTGCAGATTTGGAAAGGTTGTA
CAAGGACAGAGGGTTTCTCCCCATGCTCTTCCCATGCAACCAATTCAAGA
GCCAAGAAAGCGGTTCGCATGAAGAAATTGAGGCATTCATCAAGAGCAAG
GGCCTTGAGAACGTCGCGATCTTTTCCAAGGTGAAAGTCAACGGTAAAGA
TGCACATCCTCTCTTCCAGTGGCTGAAGGAAACCAAGCCGTCGTTTGGCT
CCTTCCTCTTTGGAGCGGATCTCAAGTGGAACTTTGCAAAGTTCCTCGTT
GACCATGAAGGGAACGTGGTGGAGTCGTACCTCCCAACCACAGCACCATC
CGCAATCGTACCAGACATTGAGAAGCTCCTCAACGATGCC
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