mRNA_M-pyrifera_M_contig71270.16994.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig71270.16994.1
Unique NamemRNA_M-pyrifera_M_contig71270.16994.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A2D5W872_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D5W872_9BACT)

HSP 1 Score: 202 bits (514), Expect = 1.180e-58
Identity = 109/178 (61.24%), Postives = 128/178 (71.91%), Query Frame = 1
Query:    1 VAAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAG 534
            VAAA  +GLP AA+++GLR F SDP DNPGRLN F + GA VLVDFAHNPDG  AL   AAALPAERRL+LLGQ+GDR+ AS R M R    +  DH++IKEL  HLRGR LGE+PA +E ELR AG   + F HA +E  AVE AL WA+ GDL+LLL H+ R AVL R+AA    G
Sbjct:  418 VAAADLMGLPAAAISAGLRDFASDPQDNPGRLNEFAVNGARVLVDFAHNPDGVSALCAMAAALPAERRLILLGQAGDRDEASIRDMTRIVWDSRPDHIIIKELPGHLRGRELGEIPAMIEDELRRAGAVAANFSHAEDEPAAVEQALAWARAGDLVLLLTHEERVAVLERVAAAAVVG 595          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A7C8CVB6_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7C8CVB6_9BACT)

HSP 1 Score: 192 bits (488), Expect = 7.290e-55
Identity = 102/173 (58.96%), Postives = 126/173 (72.83%), Query Frame = 1
Query:    1 VAAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAA 519
            VAAA  +G+  AA++ GLR F SDP DNPGRLN F +GG  VLVDFAHNP G  AL E  ++LPA+RRL+LLGQ+GDR+  S R M R    AG   +++KEL  HLRGR LGE+PA +E ELR  G P  R+ +A +EL+AV+ AL WAQPGDLLLLL HD+RD VLA++ A
Sbjct:  412 VAAAADMGIEAAAISRGLRDFKSDPKDNPGRLNEFLIGGVRVLVDFAHNPSGLAALCELTSSLPAQRRLILLGQAGDRDEESIREMVRIAWQAGPAMLLLKELDLHLRGRELGEVPAIMEDELRQLGAPADRWSYASSELQAVQEALEWAQPGDLLLLLIHDSRDEVLAKLGA 584          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A521H0X1_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A521H0X1_9BACT)

HSP 1 Score: 191 bits (485), Expect = 1.660e-54
Identity = 104/187 (55.61%), Postives = 130/187 (69.52%), Query Frame = 1
Query:    4 AAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAGWRAGTALPTA 564
            AAA  LGL   A+A+GLR F S P +NPGRLN FELGGA  +VDFAHNP G  A+ + A ++PA RR +L+GQ+GDR+  S R  AR+   A  + VVIKE+ ++LRGRA GE+P  +E ELR  G P+S   HAP+E+EAV  ALRWAQ GDLLLLL+H  R++VLA +  L   GWR G  LP A
Sbjct:  398 AAAMVLGLAPPAIAAGLRGFESTPEENPGRLNLFELGGARAIVDFAHNPHGMAAILDMALSMPARRRALLVGQAGDRDDHSIREFARSAWRACPELVVIKEMRKYLRGRAEGEVPRIIEDELRRLGAPDSAIVHAPSEMEAVRVALRWAQAGDLLLLLSHAERESVLALLRRLRDQGWRPGQPLPGA 584          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A2E1VV98_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E1VV98_9BACT)

HSP 1 Score: 188 bits (477), Expect = 1.930e-53
Identity = 103/185 (55.68%), Postives = 122/185 (65.95%), Query Frame = 1
Query:    4 AAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAGWRAGTALP 558
            A A  LG     + +GLR F +   DNPGRLNRFELGG T +VD+AHNP GFDA+F     LPA R+ VL+GQ+GDRE  S R +AR+   A  D +VIKE+  +LRGR  GE+P  +E ELR AG  E  F HA  EL+AV  A RWAQPGDLL+LL  D R  VL  IAAL AA W+ G ALP
Sbjct:  384 AIARQLGFSLEHIRAGLRGFQNSVDDNPGRLNRFELGGLTAIVDYAHNPHGFDAVFALMQQLPAARKAVLIGQAGDREDESIRALARSTWEASPDLIVIKEMHNYLRGREPGEVPGIIEDELRRAGAQEGNFSHAATELDAVRDAFRWAQPGDLLMLLCQDERARVLEWIAALQAAHWQPGDALP 568          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A2E6D817_9BACT (Mur ligase n=2 Tax=Planctomycetes TaxID=203682 RepID=A0A2E6D817_9BACT)

HSP 1 Score: 184 bits (466), Expect = 6.370e-52
Identity = 97/171 (56.73%), Postives = 115/171 (67.25%), Query Frame = 1
Query:   10 AHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAAL 522
            A  LGL   A+A+GLR+F  D ADNPGR+NRF +GG  V+VDFAHNP G +A+   A  LPA+RRLVLLGQ+GDRE  +   +AR    AGFDHV+IK++  H RGR  GE+ A +E EL  AG    R  HA +E   VE AL WAQPGDLLLLL H  R  VL RI AL
Sbjct:  386 AEGLGLDQEAIAAGLRSFRGDDADNPGRMNRFNVGGVDVIVDFAHNPHGLEAMLNMACTLPAKRRLVLLGQAGDREDEAIGELARLSAGAGFDHVIIKDMEVHSRGREKGEVVALLERELEGAGFSPERCSHAESEAAGVEQALEWAQPGDLLLLLVHADRAGVLDRIRAL 556          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A2D6HIV4_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D6HIV4_9BACT)

HSP 1 Score: 181 bits (460), Expect = 5.610e-51
Identity = 101/179 (56.42%), Postives = 120/179 (67.04%), Query Frame = 1
Query:   10 AHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAGWRAG 546
            A ALG     +A+GLRAF  D ADNPGR NRF+L G  V+VDFAHNP G  A  EAA ALPAERRLVLLGQ+GDR+  S   +AR   A G + V++K+LT H RGR  GE+ A +   L   GL E R+E A +EL AVE AL WA+ GD LLLLAH+   AV+ RI AL+A GW  G
Sbjct:  386 AEALGFSLVEIAAGLRAFSGDEADNPGRANRFDLAGVDVIVDFAHNPHGMSAFLEAAWALPAERRLVLLGQAGDRDDESILELARVVGAFGPERVILKDLTVHARGRVPGEVLALLARGLAQGGLSEERWEEATSELAAVERALEWARGGDQLLLLAHEFPCAVIERIRALEAVGWEPG 564          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A800KJZ9_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A800KJZ9_9BACT)

HSP 1 Score: 177 bits (449), Expect = 1.910e-49
Identity = 93/173 (53.76%), Postives = 117/173 (67.63%), Query Frame = 1
Query:    4 AAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAAL 522
            A A  LGL   A+ +GLR+F  D ADNPGR+NRF++GGA V+VDFAHNP G +A+   A ALPA+RRLVLLGQ+GDR+  +   +AR    +GFDHV++K++  H RGR +GE+   +  EL   G P  R   A +E   VE AL WAQPGDLLLLL H+ R  VL RI AL
Sbjct:  384 AIAEGLGLDKEAILAGLRSFRGDDADNPGRMNRFKVGGADVIVDFAHNPHGLEAMLNMACALPAKRRLVLLGQAGDRDDEAIGELARLSARSGFDHVILKDMEGHRRGREVGEVVDLLARELEEVGFPLERCSRAESEAAGVEQALEWAQPGDLLLLLVHEDRAGVLDRIRAL 556          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A2A5DFL5_9BACT (Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2A5DFL5_9BACT)

HSP 1 Score: 177 bits (449), Expect = 2.960e-49
Identity = 95/186 (51.08%), Postives = 118/186 (63.44%), Query Frame = 1
Query:    1 VAAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAGWRAGTALP 558
            +A A  LGL DA++  GLR F SDPADNPGRLN F+  G  VLVDFAHNP G  ALFE A A+  ER+LV +G +GDRE  S   +A     +G D ++IKE  +HLRGR LGE+PA +EA L A G P      A NE++A   AL WA+PGDLLLLL    R+ VL  +  +    W+AG  +P
Sbjct:  395 IAVAKELGLDDASIVEGLRTFQSDPADNPGRLNLFDFHGVKVLVDFAHNPHGLQALFEMATAMKPERKLVTVGHAGDRETESIEEVALTAWRSGVDRLIIKEQDKHLRGRELGEIPAIMEAALLADGAPAEDISRADNEMDATLQALTWAKPGDLLLLLTLQQREXVLHLLDVMQKQDWQAGQPIP 580          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A3N5Y2H7_9BACT (Mur ligase n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A3N5Y2H7_9BACT)

HSP 1 Score: 177 bits (448), Expect = 3.310e-49
Identity = 95/186 (51.08%), Postives = 120/186 (64.52%), Query Frame = 1
Query:    1 VAAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAGWRAGTALP 558
            V AA  LGLP  A+   LR FG+DPADNPGR N FE+GG  VL+D+AHNP G +AL  AAA +P+ RRLV+LGQ+GDR   + R +AR+ +A   D VV KE+  +LRGRA GE+P  +  E R  GL E++      E+ AVE AL WA+PGDLL+L  H  R  V   +  L   GW+AG  LP
Sbjct:  386 VLAAGGLGLPFEAICETLRRFGTDPADNPGRANLFEVGGVKVLMDYAHNPHGMEALAAAAAGVPSTRRLVMLGQAGDRSDEAIRDLARSVMALRPDRVVAKEMDRYLRGRAEGEIPGIMADEFRRLGLSEAQISAGDVEIRAVEKALEWARPGDLLILAVHQDRPVVATLLDRLRRTGWKAGEPLP 571          
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Match: A0A523N9A7_9BACT (Mur ligase n=1 Tax=Gemmatimonadetes bacterium TaxID=2026742 RepID=A0A523N9A7_9BACT)

HSP 1 Score: 173 bits (439), Expect = 7.920e-48
Identity = 98/186 (52.69%), Postives = 122/186 (65.59%), Query Frame = 1
Query:    1 VAAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNPDGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVIKELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWAQPGDLLLLLAHDARDAVLARIAALDAAGWRAGTALP 558
            V AA AL + D A+A  L  F     DNPGRLN F+LGGATV+VDFAHNP G  AL E A A+PA RRL L+GQ+GDR+  S   +ARA  +   D VV+KE+ ++LRGR LG +PA +E EL AAG P      A +EL  V AAL WA+ GDL+LL AH+ R  +L  +A L+ +GWR G  LP
Sbjct:  388 VGAAGALEIADDAIARALERFRPSRRDNPGRLNVFDLGGATVIVDFAHNPHGVRALGEMARAMPAARRLFLVGQAGDRDDDSILDLARAVWSMAPDCVVVKEMPKYLRGRELGVVPAMIERELAAAGAPPEAIIRAESELAGVRAALEWARVGDLVLLTAHENRTEMLDLMARLETSGWRPGETLP 573          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig71270.16994.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D5W872_9BACT1.180e-5861.24Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A7C8CVB6_9BACT7.290e-5558.96Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A521H0X1_9BACT1.660e-5455.61Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A2E1VV98_9BACT1.930e-5355.68Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A2E6D817_9BACT6.370e-5256.73Mur ligase n=2 Tax=Planctomycetes TaxID=203682 Rep... [more]
A0A2D6HIV4_9BACT5.610e-5156.42Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A800KJZ9_9BACT1.910e-4953.76Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A2A5DFL5_9BACT2.960e-4951.08Mur ligase n=1 Tax=Planctomycetes bacterium TaxID=... [more]
A0A3N5Y2H7_9BACT3.310e-4951.08Mur ligase n=1 Tax=Acidobacteria bacterium TaxID=1... [more]
A0A523N9A7_9BACT7.920e-4852.69Mur ligase n=1 Tax=Gemmatimonadetes bacterium TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig71270contigM-pyrifera_M_contig71270:3..575 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score155.2
Seed ortholog evalue3e-35
Seed eggNOG ortholog1379270.AUXF01000001_gene2523
Preferred namemurE
KEGG rclassRC00064,RC00090
KEGG koko:K01928,ko:K03802
KEGG ReactionR02788
KEGG Pathwayko00300,ko00550,map00300,map00550
Hectar predicted targeting categoryother localisation
EggNOG free text desc.UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity
EggNOG OGsCOG0769@1,COG0769@2
EC6.3.2.13,6.3.2.29,6.3.2.30
COG Functional cat.M
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01011
Exons1
Model size573
Cds size573
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816607.188852-CDS-M-pyrifera_M_contig71270:2..5751622816607.188852-CDS-M-pyrifera_M_contig71270:2..575Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig71270 3..575 +
1692278074.1882932-CDS-M-pyrifera_M_contig71270:2..5751692278074.1882932-CDS-M-pyrifera_M_contig71270:2..575Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig71270 3..575 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig71270.16994.1prot_M-pyrifera_M_contig71270.16994.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig71270 3..575 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig71270.16994.1

>prot_M-pyrifera_M_contig71270.16994.1 ID=prot_M-pyrifera_M_contig71270.16994.1|Name=mRNA_M-pyrifera_M_contig71270.16994.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=191bp
VAAAHALGLPDAALASGLRAFGSDPADNPGRLNRFELGGATVLVDFAHNP
DGFDALFEAAAALPAERRLVLLGQSGDRERASTRGMARACVAAGFDHVVI
KELTEHLRGRALGELPAEVEAELRAAGLPESRFEHAPNELEAVEAALRWA
QPGDLLLLLAHDARDAVLARIAALDAAGWRAGTALPTASD*
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mRNA from alignment at M-pyrifera_M_contig71270:3..575+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig71270.16994.1 ID=mRNA_M-pyrifera_M_contig71270.16994.1|Name=mRNA_M-pyrifera_M_contig71270.16994.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=573bp|location=Sequence derived from alignment at M-pyrifera_M_contig71270:3..575+ (Macrocystis pyrifera P11B4 male)
GTCGCGGCCGCCCACGCGCTGGGCTTGCCCGACGCCGCGCTCGCCAGCGG CCTGCGCGCGTTCGGCAGCGACCCCGCCGACAACCCCGGACGCCTGAACC GCTTCGAGCTCGGCGGCGCGACCGTGCTGGTCGACTTCGCCCACAACCCG GACGGCTTCGACGCGCTGTTCGAAGCCGCCGCAGCGCTGCCGGCCGAGCG CCGCCTGGTGCTGCTCGGCCAGTCCGGCGACCGCGAGCGCGCGTCGACCC GGGGCATGGCGCGCGCGTGCGTCGCGGCGGGCTTCGACCACGTCGTGATC AAGGAGCTGACCGAGCACCTGCGCGGCCGCGCGCTGGGCGAGCTGCCGGC CGAGGTCGAGGCCGAGCTGCGCGCCGCGGGTCTGCCGGAGTCCCGCTTCG AGCACGCGCCGAACGAGCTCGAAGCCGTCGAGGCGGCCTTGCGCTGGGCC CAGCCGGGCGACCTCTTGTTGCTGCTCGCGCACGACGCGCGCGACGCGGT GCTCGCGCGCATCGCGGCCCTCGATGCGGCGGGATGGCGCGCCGGCACTG CGTTGCCGACCGCGTCCGATTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig71270:3..575+

>mRNA_M-pyrifera_M_contig71270.16994.1 ID=mRNA_M-pyrifera_M_contig71270.16994.1|Name=mRNA_M-pyrifera_M_contig71270.16994.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1146bp|location=Sequence derived from alignment at M-pyrifera_M_contig71270:3..575+ (Macrocystis pyrifera P11B4 male)
GTCGCGGCCGCCCACGCGCTGGGCTTGCCCGACGCCGCGCTCGCCAGCGG
CCTGCGCGCGTTCGGCAGCGACCCCGCCGACAACCCCGGACGCCTGAACC
GCTTCGAGCTCGGCGGCGCGACCGTGCTGGTCGACTTCGCCCACAACCCG
GACGGCTTCGACGCGCTGTTCGAAGCCGCCGCAGCGCTGCCGGCCGAGCG
CCGCCTGGTGCTGCTCGGCCAGTCCGGCGACCGCGAGCGCGCGTCGACCC
GGGGCATGGCGCGCGCGTGCGTCGCGGCGGGCTTCGACCACGTCGTGATC
AAGGAGCTGACCGAGCACCTGCGCGGCCGCGCGCTGGGCGAGCTGCCGGC
CGAGGTCGAGGCCGAGCTGCGCGCCGCGGGTCTGCCGGAGTCCCGCTTCG
AGCACGCGCCGAACGAGCTCGAAGCCGTCGAGGCGGCCTTGCGCTGGGCC
CAGCCGGGCGACCTCTTGTTGCTGCTCGCGCACGACGCGCGCGACGCGGT
GCTCGCGCGCATCGCGGCCCTCGATGCGGCGGGATGGCGCGCCGGCACTG
CGTTGCCGACCGCGTCCGATTAGGTCGCGGCCGCCCACGCGCTGGGCTTG
CCCGACGCCGCGCTCGCCAGCGGCCTGCGCGCGTTCGGCAGCGACCCCGC
CGACAACCCCGGACGCCTGAACCGCTTCGAGCTCGGCGGCGCGACCGTGC
TGGTCGACTTCGCCCACAACCCGGACGGCTTCGACGCGCTGTTCGAAGCC
GCCGCAGCGCTGCCGGCCGAGCGCCGCCTGGTGCTGCTCGGCCAGTCCGG
CGACCGCGAGCGCGCGTCGACCCGGGGCATGGCGCGCGCGTGCGTCGCGG
CGGGCTTCGACCACGTCGTGATCAAGGAGCTGACCGAGCACCTGCGCGGC
CGCGCGCTGGGCGAGCTGCCGGCCGAGGTCGAGGCCGAGCTGCGCGCCGC
GGGTCTGCCGGAGTCCCGCTTCGAGCACGCGCCGAACGAGCTCGAAGCCG
TCGAGGCGGCCTTGCGCTGGGCCCAGCCGGGCGACCTCTTGTTGCTGCTC
GCGCACGACGCGCGCGACGCGGTGCTCGCGCGCATCGCGGCCCTCGATGC
GGCGGGATGGCGCGCCGGCACTGCGTTGCCGACCGCGTCCGATTAG
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