mRNA_M-pyrifera_M_contig6612.16052.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig6612.16052.1
Unique NamemRNA_M-pyrifera_M_contig6612.16052.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: D7FMH7_ECTSI (UDENN domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMH7_ECTSI)

HSP 1 Score: 227 bits (579), Expect = 1.550e-65
Identity = 115/128 (89.84%), Postives = 122/128 (95.31%), Query Frame = 1 
Query:  187 LRKASRLA-FSELKHDVREERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTEGGAHPLSLLDWSKFQPKQRDGRYAIAP 567
            +RK+SR+A FSELKHDVREERLYKANAAL+VAFEALE MRE+APAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFT+GGAHPLSLLDWSKFQPK RDGR +  P
Sbjct:  679 VRKSSRMASFSELKHDVREERLYKANAALMVAFEALEAMREKAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTDGGAHPLSLLDWSKFQPKHRDGRRSHEP 806
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A6H5JMA5_9PHAE (UDENN domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JMA5_9PHAE)

HSP 1 Score: 223 bits (569), Expect = 3.820e-64
Identity = 117/145 (80.69%), Postives = 130/145 (89.66%), Query Frame = 1 
Query:  136 REGAAGDTGDGLSSSGNLRKASRLA-FSELKHDVREERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTEGGAHPLSLLDWSKFQPKQRDGRYAIAP 567
            RE A+G  G G+     +RK+SR+A FS+LKHDV+EERLYKANAAL+VAFEAL+ MRE+APAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSF++GGAHPLSLLDWSKFQPK RDGR +  P
Sbjct: 1291 RETASGRIGGGVK----VRKSSRMASFSDLKHDVQEERLYKANAALMVAFEALDAMREKAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFSDGGAHPLSLLDWSKFQPKHRDGRRSHEP 1431
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A835ZGT0_9STRA (UDENN domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZGT0_9STRA)

HSP 1 Score: 122 bits (306), Expect = 8.330e-29
Identity = 68/118 (57.63%), Postives = 81/118 (68.64%), Query Frame = 1 
Query:  202 RLAFSELKH-DVREERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTEGGAHPLSLLDWSKFQPKQRDGR 552
            R+ FSE    DV EERL  A AAL VAFE +E+MR      EE VYR LI AC RCG+SE+A+ VVEMMM+ GI PDA +S CL+DAF+MD +   G  +PL LLDWSK Q +  DGR
Sbjct: 1185 RVPFSEQGEVDVAEERLAAAKAALGVAFEVVELMRRFRMEVEEGVYRGLIDACVRCGDSERAIQVVEMMMAAGIRPDAAISNCLIDAFSMDKTMAAG--NPLDLLDWSKLQSRGPDGR 1300
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A7S0C7X1_9STRA (Hypothetical protein n=1 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0C7X1_9STRA)

HSP 1 Score: 70.9 bits (172), Expect = 5.610e-11
Identity = 39/96 (40.62%), Postives = 57/96 (59.38%), Query Frame = 1 
Query:  238 EERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTE-GGAHPLSLLDWS 522
            EE    A A LV+AF+ LE+MRER    +   Y+ LI ACGRCG++E+A  ++  M   GIV D +V  CLV AF+ + ++ +  G     L +W+
Sbjct:  181 EEAKATAKAQLVLAFDILEMMRERILKADPEAYQCLIDACGRCGDTERATKLLSRMHEDGIVADGVVYACLVSAFSAESAWKKLMGKKEEDLPEWA 276
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A448ZR92_9STRA (UDENN domain-containing protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448ZR92_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 1.120e-6
Identity = 31/78 (39.74%), Postives = 49/78 (62.82%), Query Frame = 1 
Query:  262 AALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTEGGA 495
            A L +AF+ALE+M  R    +  V++SL+ ACGRCGN+++A+A++E+M   G+V D  V    + +FA    + E  A
Sbjct: 1000 AQLDLAFDALEMMETRGLNTDPDVFKSLMEACGRCGNTKRALALIEIMKRDGLVADNDVLIFFMASFAHCRPYEEASA 1077
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A7S1TYQ9_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TYQ9_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 2.090e-6
Identity = 33/97 (34.02%), Postives = 51/97 (52.58%), Query Frame = 1 
Query:  229 DVREERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDSFTEGGAH-PLSLLD 516
            D  EER  K N  L VAFE +E + +   + +   ++SLI ACGRCGN E+A+ V++ +    I     +   L+ AF    +   G  + PL ++D
Sbjct:  934 DAAEERQVKMNMMLEVAFEVIEAVLDLGASLDSHAFQSLIHACGRCGNVERAIKVLDFLEQANIQVTQTLRTALLIAFDRSGNAASGSGNLPLQIVD 1030
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A7S2Y8L2_9STRA (Hypothetical protein n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S2Y8L2_9STRA)

HSP 1 Score: 57.0 bits (136), Expect = 3.620e-6
Identity = 29/80 (36.25%), Postives = 47/80 (58.75%), Query Frame = 1 
Query:  238 EERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDS 477
            EE    A A L +AFE L+ +  R    +   Y+SL+ ACGRCG++++A  ++++M   G + D  +  C V AFA ++S
Sbjct:  577 EEAKEVATAQLDLAFEVLKTVSMRKLPADLDSYKSLMEACGRCGDTQRARILIDLMKKDGFILDGEILSCFVAAFAHEES 656
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: B7FVV2_PHATC (Predicted protein n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7FVV2_PHATC)

HSP 1 Score: 55.1 bits (131), Expect = 1.710e-5
Identity = 29/80 (36.25%), Postives = 47/80 (58.75%), Query Frame = 1 
Query:  238 EERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFAMDDS 477
            EE    A+A L +AFEAL  M  R    +   Y+SL+ ACGRCG++E+A+ ++++M   G   D+ +    + AFA + +
Sbjct:  904 EEAKEVASAQLDLAFEALTTMSLRGLKTDSDAYKSLMEACGRCGDTERALKLMQVMKVDGFFADSEILAWFIAAFAQESA 983
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BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Match: A0A7S4AQ15_9STRA (Hypothetical protein n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AQ15_9STRA)

HSP 1 Score: 53.1 bits (126), Expect = 7.570e-5
Identity = 29/76 (38.16%), Postives = 46/76 (60.53%), Query Frame = 1 
Query:  238 EERLYKANAALVVAFEALEVMRERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCLVDAFA 465
            EE    A A L +AF+ L++M  R    +  V++SL+ ACGRCGN+++A+ ++E M   G+V D  V    + +FA
Sbjct:  706 EEAREVAVAQLDLAFDGLQMMETRGLTTDPDVFKSLMEACGRCGNTKRALELIETMKRDGLVADNDVLVFFMASFA 781
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig6612.16052.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
D7FMH7_ECTSI1.550e-6589.84UDENN domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A6H5JMA5_9PHAE3.820e-6480.69UDENN domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A835ZGT0_9STRA8.330e-2957.63UDENN domain-containing protein n=1 Tax=Tribonema ... [more]
A0A7S0C7X1_9STRA5.610e-1140.63Hypothetical protein n=1 Tax=Proboscia inermis Tax... [more]
A0A448ZR92_9STRA1.120e-639.74UDENN domain-containing protein n=1 Tax=Pseudo-nit... [more]
A0A7S1TYQ9_9STRA2.090e-634.02Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
A0A7S2Y8L2_9STRA3.620e-636.25Hypothetical protein n=1 Tax=Amphiprora paludosa T... [more]
B7FVV2_PHATC1.710e-536.25Predicted protein n=1 Tax=Phaeodactylum tricornutu... [more]
A0A7S4AQ15_9STRA7.570e-538.16Hypothetical protein n=1 Tax=Pseudo-nitzschia aust... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig6612contigM-pyrifera_M_contig6612:1871..4204 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score228.0
Seed ortholog evalue3.6e-57
Seed eggNOG ortholog2880.D7FMH7
KEGG koko:K20163
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Rab guanyl-nucleotide exchange factor activity
EggNOG OGsKOG2127@1,KOG2127@2759
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Exons3
Model size567
Cds size567
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig6612.16052.1prot_M-pyrifera_M_contig6612.16052.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig6612 1871..4204 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816550.6996162-CDS-M-pyrifera_M_contig6612:1870..21351622816550.6996162-CDS-M-pyrifera_M_contig6612:1870..2135Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig6612 1871..2135 +
1692278041.5381517-CDS-M-pyrifera_M_contig6612:1870..21351692278041.5381517-CDS-M-pyrifera_M_contig6612:1870..2135Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig6612 1871..2135 +
1622816550.712207-CDS-M-pyrifera_M_contig6612:2384..25181622816550.712207-CDS-M-pyrifera_M_contig6612:2384..2518Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig6612 2385..2518 +
1692278041.5502207-CDS-M-pyrifera_M_contig6612:2384..25181692278041.5502207-CDS-M-pyrifera_M_contig6612:2384..2518Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig6612 2385..2518 +
1622816550.7227433-CDS-M-pyrifera_M_contig6612:4036..42041622816550.7227433-CDS-M-pyrifera_M_contig6612:4036..4204Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig6612 4037..4204 +
1692278041.560123-CDS-M-pyrifera_M_contig6612:4036..42041692278041.560123-CDS-M-pyrifera_M_contig6612:4036..4204Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig6612 4037..4204 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig6612.16052.1

>prot_M-pyrifera_M_contig6612.16052.1 ID=prot_M-pyrifera_M_contig6612.16052.1|Name=mRNA_M-pyrifera_M_contig6612.16052.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=189bp
PSVESASQSSLPAVVTMSTKGVVHGAGRPSGRDGDVGEQNADSEGREGAA
GDTGDGLSSSGNLRKASRLAFSELKHDVREERLYKANAALVVAFEALEVM
RERAPAGEEAVYRSLISACGRCGNSEKAMAVVEMMMSTGIVPDAMVSRCL
VDAFAMDDSFTEGGAHPLSLLDWSKFQPKQRDGRYAIAP
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mRNA from alignment at M-pyrifera_M_contig6612:1871..4204+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig6612.16052.1 ID=mRNA_M-pyrifera_M_contig6612.16052.1|Name=mRNA_M-pyrifera_M_contig6612.16052.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2334bp|location=Sequence derived from alignment at M-pyrifera_M_contig6612:1871..4204+ (Macrocystis pyrifera P11B4 male)
CCTTCTGTCGAATCCGCTTCGCAGTCGTCTCTGCCGGCGGTGGTCACGAT
GAGCACAAAGGGGGTTGTTCACGGTGCGGGCCGACCATCGGGACGGGATG
GCGATGTTGGCGAGCAGAACGCCGACTCGGAGGGGCGGGAAGGGGCGGCT
GGGGACACTGGGGACGGGTTGAGCTCGAGCGGGAACTTGAGGAAGGCGTC
GCGTCTTGCGTTCAGCGAGCTCAAGCACGACGTGCGGGAGGAGAGGCTGT
ACAAGGCTAACGCAGGTGTGCGTGCGCTCGTGTTTTGTGTTCAATTGCCC
CGTGTTTTGTTTGGTGCTCGAGATTCCCTCTGAATGACACGGTATTGCCA
CAAAAAAAAGTATTTACCGTGTAGGATATGCATCATCTACGCATATTTTC
CAACCCACTATTCTGTACAATCTAGGTCAGGCAGATCATGGTCTGGCCAA
CGTGTAGATGCATTGAAAAAAAAACTCACGTGCATCTTGATTTTCCCCCA
CCCCGTCCCTGCAGCTCTTGTGGTGGCCTTCGAGGCGCTGGAGGTAATGC
GCGAGAGAGCACCGGCGGGGGAAGAGGCCGTGTATCGGAGCCTAATTTCC
GCCTGCGGACGGTGTGGGAACTCCGAGAAGGCCATGGCGGTTGTTGAGGT
GATATTTTTTTACGTTTTATTGTTGTAAGAAACACAGGATACCATCAGCC
GTCGTCGACAACGTGTCGGTTGATTGAACATTTGTATGTCCTTTGTCCTA
TGGTAGCAAGATCCCTATCTACGTGATAGGAAACCCGGGGATGCACTCAA
CATGTCCCCTGACGACCACAGCCCGTGAGAAAGTGCGCGCCCTAATAGGG
AATGGTGGTGCAGCATGTACGTACACGACACCACTTGAGAGGCTCTTATT
TTTGGCCTGGCTCAAGTTCCTGTTGTTATAATATGTGAAGCCAGGTGTGG
TCTACTGTACAGTTATTGAAGTAGTACAAAATGTCCTTCTTGCTCATCGT
GCACATATTGAGCCATTTTTCCAGGGCTGTTCATGGGTCAGGTCAAACCT
CGCGGGTCGAGTCGGGCCGGGTTGGGTCAAGCCGACCTGACCCGACCCGT
GAGATTTCGAAAACCCGCTGACCCGACCCGACCCGACCCGACCGGACCCG
TGAGATTTGTAGACTCCCTGAATCCGACCCGTGTTTCGGATCATGTAGAG
GTCTGAAATATCTTGTGTCTCCGTGGTTCTTGATTACTGTCGCGTACTGG
TATTGGTTTTAGCAGCTTCCCGGAAATAATCCAGGGTCAAGAGAACACAC
TCTTGATGACGAAGCGCTTTTAGTGATCCGGAAGACGCCCAATGACACGC
CCCGCAATGTCTGCGCAAAAGCCTCCTAGTACTCGTGAGGAGTATTCGTC
CTTCGCTTCGGCTGAGACTGTTTTGGCCTTAGGCCAGGCCTTTCGTCGTC
TGTATACGTGCTTATTCTTGATTGTGGCAGTGCGGGCATAACGCAGTGGT
GGGCAACAACGGCAGAAAAGAACCGCTTCCATGTACCCGTGCGAGTTTTT
TTTTCAACTTTTGACTGTATGGACCCAAAACTTGGGAAAGCGGTGACTCG
TTTTTTTGGCATTGTTGTAGCTTGTTGTTGCTGCTGCTGCATTGAATTTT
TTTTTCTTCGAGCAACGCGTTGTGGGTTGATGATGGTGCGTGGGTGTGGC
AGGCAGAGACCAGCCGGGATGGTTCGCGCACTGCTCTGCGCTTACACCTT
CATGCAGAGCTCCTCCGGGGGGACGTACGGTAGCAACCAGGACCAAATAC
GGCGTGTGAACATAGGGGGGGGGGGGTTCATGTTTTTTGGATCTGTCGTA
GTTGCTGATTACTATGCCCCCCGGTAACTGAACTCTCGTGACTCGTCGTT
CTTCGAGACCATTTAAGTGTGACGTACGTAGAAATAGCTATAAACTCGCC
TGGCGCGGTCGGTCGCTAGCGCTAAAACGTTTCGCGAAAATACTCACATA
TGAATTCACATTCCGTCTCTATTTTTTTCTTGTCACAAGCAAACGATTCG
ATAACTCGCGCCGAGGCTAACCCCGCTCCCCACTCTCGTCCCACCGTCGT
CCCACCCCTACCCCACCCTACCTTCCCCAACCAATGGTCGCATTGTTTTC
GCGCCTCGTTGGGAAGATGATGATGTCGACCGGCATCGTTCCCGACGCCA
TGGTGTCGCGGTGTCTGGTTGACGCGTTCGCCATGGACGACAGCTTCACC
GAAGGCGGTGCTCACCCCCTCTCCCTCCTGGACTGGTCCAAGTTTCAGCC
GAAGCAGCGGGACGGACGGTACGCCATCGCGCCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig6612:1871..4204+

>mRNA_M-pyrifera_M_contig6612.16052.1 ID=mRNA_M-pyrifera_M_contig6612.16052.1|Name=mRNA_M-pyrifera_M_contig6612.16052.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1134bp|location=Sequence derived from alignment at M-pyrifera_M_contig6612:1871..4204+ (Macrocystis pyrifera P11B4 male)
CCTTCTGTCGAATCCGCTTCGCAGTCGTCTCTGCCGGCGGTGGTCACGAT
GAGCACAAAGGGGGTTGTTCACGGTGCGGGCCGACCATCGGGACGGGATG
GCGATGTTGGCGAGCAGAACGCCGACTCGGAGGGGCGGGAAGGGGCGGCT
GGGGACACTGGGGACGGGTTGAGCTCGAGCGGGAACTTGAGGAAGGCGTC
GCGTCTTGCGTTCAGCGAGCTCAAGCACGACGTGCGGGAGGAGAGGCTGT
ACAAGGCTAACGCAGCCTTCTGTCGAATCCGCTTCGCAGTCGTCTCTGCC
GGCGGTGGTCACGATGAGCACAAAGGGGGTTGTTCACGGTGCGGGCCGAC
CATCGGGACGGGATGGCGATGTTGGCGAGCAGAACGCCGACTCGGAGGGG
CGGGAAGGGGCGGCTGGGGACACTGGGGACGGGTTGAGCTCGAGCGGGAA
CTTGAGGAAGGCGTCGCGTCTTGCGTTCAGCGAGCTCAAGCACGACGTGC
GGGAGGAGAGGCTGTACAAGGCTAACGCAGCTCTTGTGGTGGCCTTCGAG
GCGCTGGAGGTAATGCGCGAGAGAGCACCGGCGGGGGAAGAGGCCGTGTA
TCGGAGCCTAATTTCCGCCTGCGGACGGTGTGGGAACTCCGAGAAGGCCA
TGGCGGTTGTTGAGCTCTTGTGGTGGCCTTCGAGGCGCTGGAGGTAATGC
GCGAGAGAGCACCGGCGGGGGAAGAGGCCGTGTATCGGAGCCTAATTTCC
GCCTGCGGACGGTGTGGGAACTCCGAGAAGGCCATGGCGGTTGTTGAGAT
GATGATGTCGACCGGCATCGTTCCCGACGCCATGGTGTCGCGGTGTCTGG
TTGACGCGTTCGCCATGGACGACAGCTTCACCGAAGGCGGTGCTCACCCC
CTCTCCCTCCTGGACTGGTCCAAGTTTCAGCCGAAGCAGCGGGACGGACG
GTACGCCATCGCGCCTATGATGATGTCGACCGGCATCGTTCCCGACGCCA
TGGTGTCGCGGTGTCTGGTTGACGCGTTCGCCATGGACGACAGCTTCACC
GAAGGCGGTGCTCACCCCCTCTCCCTCCTGGACTGGTCCAAGTTTCAGCC
GAAGCAGCGGGACGGACGGTACGCCATCGCGCCT
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