mRNA_M-pyrifera_M_contig1333.5915.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig1333.5915.1
Unique NamemRNA_M-pyrifera_M_contig1333.5915.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: D7G5C0_ECTSI (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5C0_ECTSI)

HSP 1 Score: 197 bits (500), Expect = 2.040e-63
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1 
Query:    1 AAVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKKXXXXXXXXXXXXFKKSKKPKA 336
            AAVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKKXXXXXXXXXXXXFKKSKKPKA
Sbjct:    2 AAVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKKXXXXXXXXXXXXFKKSKKPKA 113
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: A0A835ZED0_9STRA (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZED0_9STRA)

HSP 1 Score: 152 bits (384), Expect = 1.110e-45
Identity = 74/88 (84.09%), Postives = 80/88 (90.91%), Query Frame = 1 
Query:    4 AVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKM 267
            AVGVPIKLLHEGEGH +T+EMKNGEIYRG L EAEDTMN F+T VTMT RDGRVSKLEQVYLRGSHIK ++LPD+LKNAPVFK VQKM
Sbjct:    6 AVGVPIKLLHEGEGHVITIEMKNGEIYRGRLMEAEDTMNSFITEVTMTARDGRVSKLEQVYLRGSHIKFIILPDLLKNAPVFKGVQKM 93
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: A0A7S2S181_9STRA (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2S181_9STRA)

HSP 1 Score: 139 bits (351), Expect = 1.070e-40
Identity = 63/89 (70.79%), Postives = 78/89 (87.64%), Query Frame = 1 
Query:    7 VGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKK 273
            +GVPI++LHEGEGH ++VE++NGEIYRG L EAEDTMNCF+  VTMT RDGRVSKLEQVY+RGS +KL++LP+VLK++P F  VQ MKK
Sbjct:    6 IGVPIRILHEGEGHTISVELRNGEIYRGTLKEAEDTMNCFLENVTMTARDGRVSKLEQVYIRGSQVKLIILPEVLKHSPAFNKVQTMKK 94
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: A0A6S9H3G1_HETAK (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6S9H3G1_HETAK)

HSP 1 Score: 136 bits (342), Expect = 2.360e-39
Identity = 62/85 (72.94%), Postives = 75/85 (88.24%), Query Frame = 1 
Query:    7 VGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQ 261
            +GVP+KLLHE EGH VT E+KNGEIYRG L+EAEDTMNC M+ VT T R+G+VSKLE VY+RGSH+KL++LPD+L+NAPVFK VQ
Sbjct:    6 IGVPVKLLHEAEGHVVTAELKNGEIYRGMLDEAEDTMNCAMSNVTFTARNGKVSKLESVYIRGSHVKLLILPDMLRNAPVFKKVQ 90
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: K3WSP0_GLOUD (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WSP0_GLOUD)

HSP 1 Score: 135 bits (339), Expect = 7.180e-39
Identity = 61/89 (68.54%), Postives = 77/89 (86.52%), Query Frame = 1 
Query:    7 VGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKK 273
            VGVPI LLHEGEG  VT+E+KNGEIYRGHL E+ED+MNC ++ V +T RDG+ +KLEQVY+RGSHIKL++LPD+LKN+P+F  VQ +KK
Sbjct:    5 VGVPIALLHEGEGRNVTIELKNGEIYRGHLEESEDSMNCQLSNVVLTQRDGQKAKLEQVYVRGSHIKLIILPDILKNSPLFSKVQALKK 93
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: F0Y2Z6_AURAN (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y2Z6_AURAN)

HSP 1 Score: 132 bits (332), Expect = 8.100e-38
Identity = 63/95 (66.32%), Postives = 81/95 (85.26%), Query Frame = 1 
Query:    4 AVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIK-----LVVLPDVLKNAPVFKPVQKMKK 273
            AVGVPIKLLHEGEGH VT+E+KNGEIYRGHL+E+E+TMNC +T V +T RDGRV+KLE VY+RGS IK      ++LPD+LK++PVF+ V+++KK
Sbjct:    7 AVGVPIKLLHEGEGHMVTIELKNGEIYRGHLDESEETMNCLVTDVVVTARDGRVTKLENVYIRGSQIKRERRAFMILPDLLKHSPVFEKVKELKK 101
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: A0A485LB96_9STRA (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LB96_9STRA)

HSP 1 Score: 132 bits (331), Expect = 1.340e-37
Identity = 58/89 (65.17%), Postives = 78/89 (87.64%), Query Frame = 1 
Query:    7 VGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKK 273
            VGVP+ LLHEGEGH +T+E+KNGE+YRGHL E+ED+MNC +  VT+TGRDG++S+LEQVY+RGS +KL +LPD+L+ +P+FK VQ +KK
Sbjct:    5 VGVPLSLLHEGEGHQITLELKNGEVYRGHLVESEDSMNCQLKQVTLTGRDGQISRLEQVYVRGSQVKLFILPDMLQKSPLFKKVQALKK 93
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: A0A5D6XZW3_9STRA (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XZW3_9STRA)

HSP 1 Score: 132 bits (331), Expect = 1.560e-37
Identity = 64/90 (71.11%), Postives = 75/90 (83.33%), Query Frame = 1 
Query:    4 AVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKK 273
            +VGVPI LLHEGEG  VTVE+KNGEIYRGHL E+ED+MNC M  V +T RDG+  KLEQVY+RGSHIKLV+LPDVLKN+P+   VQ +KK
Sbjct:    5 SVGVPIALLHEGEGRSVTVELKNGEIYRGHLLESEDSMNCQMRDVVLTQRDGQKRKLEQVYVRGSHIKLVILPDVLKNSPLLAKVQALKK 94
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BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: T0QL39_SAPDV (Small nuclear ribonucleoprotein Sm D3 n=2 Tax=Saprolegnia TaxID=4769 RepID=T0QL39_SAPDV)

HSP 1 Score: 131 bits (330), Expect = 1.630e-37
Identity = 57/90 (63.33%), Postives = 79/90 (87.78%), Query Frame = 1 
Query:    4 AVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKK 273
            ++GVP+ LLHEGEGH +T+E+KNGEIY+GHL EAED+MNC +  VT+TGRDG++S+LEQVY+RGS +KL +LPD+L+ +P+FK +Q +KK
Sbjct:    4 SLGVPLSLLHEGEGHTITLELKNGEIYKGHLMEAEDSMNCQLKDVTLTGRDGQLSRLEQVYIRGSQVKLFILPDMLQKSPLFKKIQALKK 93
[Close]
BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Match: A0A1V9ZC26_9STRA (Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZC26_9STRA)

HSP 1 Score: 131 bits (330), Expect = 1.630e-37
Identity = 58/90 (64.44%), Postives = 79/90 (87.78%), Query Frame = 1 
Query:    4 AVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTGRDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKK 273
            ++GVP+ LLHEGEGH +T+E+KNGEIY+GHL EAED+MNC +  VT+TGRDG++S+LEQVY+RGS +KL +LPD+L+ +P+FK VQ +KK
Sbjct:    4 SLGVPLSLLHEGEGHTITLELKNGEIYKGHLLEAEDSMNCQLKDVTLTGRDGQLSRLEQVYIRGSQVKLFILPDMLQKSPLFKKVQALKK 93
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig1333.5915.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5C0_ECTSI2.040e-63100.00Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Ecto... [more]
A0A835ZED0_9STRA1.110e-4584.09Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Trib... [more]
A0A7S2S181_9STRA1.070e-4070.79Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Rhiz... [more]
A0A6S9H3G1_HETAK2.360e-3972.94Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Hete... [more]
K3WSP0_GLOUD7.180e-3968.54Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Glob... [more]
F0Y2Z6_AURAN8.100e-3866.32Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Aure... [more]
A0A485LB96_9STRA1.340e-3765.17Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Apha... [more]
A0A5D6XZW3_9STRA1.560e-3771.11Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Pyth... [more]
T0QL39_SAPDV1.630e-3763.33Small nuclear ribonucleoprotein Sm D3 n=2 Tax=Sapr... [more]
A0A1V9ZC26_9STRA1.630e-3764.44Small nuclear ribonucleoprotein Sm D3 n=1 Tax=Thra... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig1333contigM-pyrifera_M_contig1333:15145..16396 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score137.5
Seed ortholog evalue3.9e-30
Seed eggNOG ortholog65071.PYU1_T007984
Preferred nameSMD3
KEGG koko:K07442,ko:K11088,ko:K18738
KEGG Pathwayko03040,ko04139,ko05322,map03040,map04139,map05322
KEGG ModuleM00351,M00352,M00354,M00355,M00398
Hectar predicted targeting categoryother localisation
GOsGO:0000243,GO:0000245,GO:0000375,GO:0000377,GO:0000387,GO:0000395,GO:0000398,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005681,GO:0005682,GO:0005684,GO:0005685,GO:0005686,GO:0005687,GO:0005689,GO:0005737,GO:0006139,GO:0006325,GO:0006342,GO:0006355,GO:0006376,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0006996,GO:0008150,GO:0008152,GO:0008380,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010558,GO:0010605,GO:0010629,GO:0016043,GO:0016070,GO:0016071,GO:0016458,GO:0019219,GO:0019222,GO:0022607,GO:0022613,GO:0022618,GO:0030532,GO:0030702,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0032991,GO:0034622,GO:0034641,GO:0034715,GO:0034719,GO:0040029,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0045292,GO:0045814,GO:0045892,GO:0045934,GO:0046483,GO:0046540,GO:0048519,GO:0048523,GO:0050789,GO:0050794,GO:0051171,GO:0051172,GO:0051252,GO:0051253,GO:0051276,GO:0060255,GO:0065003,GO:0065007,GO:0071004,GO:0071010,GO:0071011,GO:0071013,GO:0071014,GO:0071704,GO:0071826,GO:0071840,GO:0080090,GO:0090304,GO:0097159,GO:0097525,GO:0097526,GO:0120114,GO:1901360,GO:1901363,GO:1902494,GO:1902679,GO:1903506,GO:1903507,GO:1990141,GO:1990904,GO:2000112,GO:2000113,GO:2001141
EggNOG free text desc.Small nuclear ribonucleoprotein Sm D3. Source PGD
EggNOG OGs1MHFN@121069,COG1958@1,KOG3172@2759
Ec32 ortholog descriptionSmall nuclear ribonucleoprotein-associated protein D3
Ec32 orthologEc-14_006960.1
EC2.1.1.219,2.1.1.220
COG Functional cat.A
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03016,ko03019,ko03032,ko03041
Exons2
Model size339
Cds size339
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815813.826144-CDS-M-pyrifera_M_contig1333:15144..153471622815813.826144-CDS-M-pyrifera_M_contig1333:15144..15347Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig1333 15145..15347 -
1692277583.5386348-CDS-M-pyrifera_M_contig1333:15144..153471692277583.5386348-CDS-M-pyrifera_M_contig1333:15144..15347Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig1333 15145..15347 -
1622815813.8373747-CDS-M-pyrifera_M_contig1333:16260..163961622815813.8373747-CDS-M-pyrifera_M_contig1333:16260..16396Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig1333 16261..16396 -
1692277583.5518036-CDS-M-pyrifera_M_contig1333:16260..163961692277583.5518036-CDS-M-pyrifera_M_contig1333:16260..16396Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig1333 16261..16396 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig1333.5915.1prot_M-pyrifera_M_contig1333.5915.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig1333 15145..16396 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig1333.5915.1

>prot_M-pyrifera_M_contig1333.5915.1 ID=prot_M-pyrifera_M_contig1333.5915.1|Name=mRNA_M-pyrifera_M_contig1333.5915.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=113bp
AAVGVPIKLLHEGEGHGVTVEMKNGEIYRGHLNEAEDTMNCFMTGVTMTG
RDGRVSKLEQVYLRGSHIKLVVLPDVLKNAPVFKPVQKMKKKDEEAGGGR
GGGFKKSKKPKA*
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mRNA from alignment at M-pyrifera_M_contig1333:15145..16396-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig1333.5915.1 ID=mRNA_M-pyrifera_M_contig1333.5915.1|Name=mRNA_M-pyrifera_M_contig1333.5915.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1252bp|location=Sequence derived from alignment at M-pyrifera_M_contig1333:15145..16396- (Macrocystis pyrifera P11B4 male)
GCAGCTGTGGGAGTGCCGATCAAACTCCTCCACGAGGGCGAGGGCCACGG
GGTCACGGTGGAGATGAAAAACGGGGAGATCTACCGGGGACACTTAAACG
AGGCCGAAGACACCATGAATTGTTTCATGACGGGAGGTGAGACCGCGCTC
TCCAAGTTTGTCTACACTGCTTTGCAATCGGCACTGTGCGAGCACTACGA
CTGCGAGGTATAATTACGGGCACTATTTACCTGTTATTCAAACTTACGGA
CGAGTACGACCATGGATTAGTCTACGTCAGTAAGATTGCTGCAACACGGA
TGCCTGGTATACCTGGTATGTGATATAATACTCATTGTATTGTGCTATTA
CGGGGGACCATAGTAAATAGGACCTACGGTACACACAAGAACCTATATGT
ATTCCCTATTTTTACTAACAATATTGGGTCCGATTTACTATGGTCCCCTG
CAATCCTATTGTAGACACGAAAACGAATACGAGTAAGTAGCAGGTAAGGA
AGGTATACCAGGTACCTTCCAAGATTGTTCCTTCGGGACTATGGTTGACT
TCAAATCCCGGGGGTGTCGCAAGGTGAAAACGGAGTAAACCACACAGCAC
AGCAGGCGTTCGTTCCTCGTACCAGCTATTAATACTACCATAGTACGTAC
GGTAGTGTACGCGGCTTTTTGTTTTAAGGAATCGTATGGGATAAAGTATT
GAAACCAGCTTTGGGGTTAGGGTTAGGGTTAGGGTTAGGGTCTGCGTACC
CCCTTCTGTTCTTTTTCCTAGATACACTTAAAACCCTGCGTAGGCGGTCC
AGTACGTAGCCACCGGGCAAGAACGAAGAGCCAAAAAAAACTCTTCGAAG
CTACAGGAAAAAAATAGGCACCGGGGAATGAAACGCATACGGTTCCATTC
CGGGAGTGGTCGAGCGACGTACGTATGAATATCCGTAGTCAGTACCGATG
GATGCCGCGCATGTTATGAAGCGTTTGCCCCAACGACAATCTCAACACTC
ATGTGTGCTCTCTCTCCGTGCCCTCGCGGGATAATTGTTATGATGACAGT
GACCATGACCGGGCGAGATGGGCGCGTGAGCAAGCTGGAGCAGGTCTATC
TTCGCGGGAGCCACATCAAGCTAGTAGTATTGCCCGACGTGCTCAAGAAC
GCACCGGTATTTAAGCCTGTCCAGAAGATGAAGAAGAAGGACGAGGAGGC
AGGGGGCGGGCGAGGAGGGGGCTTCAAAAAGTCCAAAAAGCCCAAGGCAT
AG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig1333:15145..16396-

>mRNA_M-pyrifera_M_contig1333.5915.1 ID=mRNA_M-pyrifera_M_contig1333.5915.1|Name=mRNA_M-pyrifera_M_contig1333.5915.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=678bp|location=Sequence derived from alignment at M-pyrifera_M_contig1333:15145..16396- (Macrocystis pyrifera P11B4 male)
GCAGCTGTGGGAGTGCCGATCAAACTCCTCCACGAGGGCGAGGGCCACGG
GGTCACGGTGGAGATGAAAAACGGGGAGATCTACCGGGGACACTTAAACG
AGGCCGAAGACACCATGAATTGTTTCATGACGGGAGGCAGCTGTGGGAGT
GCCGATCAAACTCCTCCACGAGGGCGAGGGCCACGGGGTCACGGTGGAGA
TGAAAAACGGGGAGATCTACCGGGGACACTTAAACGAGGCCGAAGACACC
ATGAATTGTTTCATGACGGGAGTGACCATGACCGGGCGAGATGGGCGCGT
GAGCAAGCTGGAGCAGGTCTATCTTCGCGGGAGCCACATCAAGCTAGTAG
TATTGCCCGACGTGCTCAAGAACGCACCGGTATTTAAGCCTGTCCAGAAG
ATGAAGAAGAAGGACGAGGAGGCAGGGGGCGGGCGAGGAGGGGGCTTCAA
AAAGTCCAAAAAGCCCAAGGCATAGTGACCATGACCGGGCGAGATGGGCG
CGTGAGCAAGCTGGAGCAGGTCTATCTTCGCGGGAGCCACATCAAGCTAG
TAGTATTGCCCGACGTGCTCAAGAACGCACCGGTATTTAAGCCTGTCCAG
AAGATGAAGAAGAAGGACGAGGAGGCAGGGGGCGGGCGAGGAGGGGGCTT
CAAAAAGTCCAAAAAGCCCAAGGCATAG
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