mRNA_M-pyrifera_M_contig127931.5626.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig127931.5626.1
Unique NamemRNA_M-pyrifera_M_contig127931.5626.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: UPI000C6ECD94 (bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase-like n=1 Tax=Centruroides sculpturatus TaxID=218467 RepID=UPI000C6ECD94)

HSP 1 Score: 180 bits (456), Expect = 1.160e-52
Identity = 85/136 (62.50%), Postives = 104/136 (76.47%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            S+PIVLA+S E +E +    A    AL +NG +  +LRN E +EHRKEERC+RQFGT++ GHPYV+ +  SGDWLVGGDL +  R+ WNDGLDKYRLTPN+LR +F   GAD V+AFQLRNPIHNGHALLM  TR+
Sbjct:   85 SIPIVLALSKEDKERLEDCSA---FALRYNGKSYAILRNPEFYEHRKEERCSRQFGTSNEGHPYVKMIYESGDWLVGGDLEVLERICWNDGLDKYRLTPNELRSKFKKMGADAVFAFQLRNPIHNGHALLMQQTRE 217
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A1W0WTB0_HYPDU (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase n=2 Tax=Hypsibius dujardini TaxID=232323 RepID=A0A1W0WTB0_HYPDU)

HSP 1 Score: 182 bits (462), Expect = 7.600e-52
Identity = 86/136 (63.24%), Postives = 105/136 (77.21%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            SVPIVLA S   +E +    A   + L  +G  V +LRN E +EHRKEERCAR FGTT  GHPY++ +M SGDWLVGGDL +F R++WNDGLD+YRL PN+LR++FT  GAD V+AFQLRNPIHNGHALLM+DTR+
Sbjct:  299 SVPIVLAASTADKERLHGEKA---ITLVHSGKPVAILRNPEFYEHRKEERCARTFGTTHAGHPYIKMIMDSGDWLVGGDLEVFERIRWNDGLDQYRLKPNELRKKFTELGADAVFAFQLRNPIHNGHALLMSDTRR 431
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A3B4CB60_PYGNA (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2-like n=3 Tax=Serrasalmidae TaxID=42495 RepID=A0A3B4CB60_PYGNA)

HSP 1 Score: 181 bits (460), Expect = 1.440e-51
Identity = 86/136 (63.24%), Postives = 107/136 (78.68%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            S+PIVL V+ E +ES+T +   A+VAL + G  V VLRN E F+HRKEERCARQ+GTT   HPY++ VM SGDWLVGGDL +  R++WNDGLD+YRLTP +LR++F    AD V+AFQLRNP+HNGHALLM DTR+
Sbjct:  294 SIPIVLPVTMETKESLTGS---AAVALEYQGRRVAVLRNPEFFQHRKEERCARQWGTTCTKHPYIKMVMESGDWLVGGDLEVLERIKWNDGLDQYRLTPQELRKKFKEMKADAVFAFQLRNPVHNGHALLMTDTRR 426
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A6A4WVU4_AMPAM (Sulfate adenylyltransferase n=1 Tax=Amphibalanus amphitrite TaxID=1232801 RepID=A0A6A4WVU4_AMPAM)

HSP 1 Score: 174 bits (442), Expect = 3.230e-51
Identity = 84/136 (61.76%), Postives = 102/136 (75.00%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            SVPIVLAV    +E +    A   + L +NG  V +LR  E FEHRKEERCARQ+GTT+ GHPYV+ V+ SGDWLVGGDL +  R++W+DGLD+YRLTP +LR+ F    AD V+AFQLRNPIHNGHALLM DTR+
Sbjct:   24 SVPIVLAVQTADKERLAGQSA---ITLRYNGRPVAILRKPEFFEHRKEERCARQWGTTNPGHPYVKMVLDSGDWLVGGDLDVLGRIKWDDGLDQYRLTPTELRQRFRELEADAVFAFQLRNPIHNGHALLMQDTRR 156
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: UPI00143A27C2 (bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2-like n=1 Tax=Bombus vancouverensis nearcticus TaxID=2705178 RepID=UPI00143A27C2)

HSP 1 Score: 177 bits (449), Expect = 3.280e-51
Identity = 83/136 (61.03%), Postives = 103/136 (75.74%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            S+PIVL VS E +ES T A A   + L +NG  + +LR  E F HRKEERC+R+FGT D+GHPYV  +  SGDWLVGG+L +  R++WNDGLDKYRLTPN++R++     AD V+AFQLRNPIHNGHALLM DTR+
Sbjct:  125 SIPIVLPVSTEQKESYTTAPA---LTLKYNGQDIAILRRPEFFAHRKEERCSREFGTNDLGHPYVRMIHESGDWLVGGELEVLERIKWNDGLDKYRLTPNEIRKKCREMEADAVFAFQLRNPIHNGHALLMQDTRR 257
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: UPI000C6CD104 (bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase-like n=1 Tax=Centruroides sculpturatus TaxID=218467 RepID=UPI000C6CD104)

HSP 1 Score: 180 bits (456), Expect = 5.300e-51
Identity = 85/136 (62.50%), Postives = 104/136 (76.47%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            S+PIVLA+S E +E +    A    AL +NG +  +LRN E +EHRKEERC+RQFGT++ GHPYV+ +  SGDWLVGGDL +  R+ WNDGLDKYRLTPN+LR +F   GAD V+AFQLRNPIHNGHALLM  TR+
Sbjct:  292 SIPIVLALSKEDKERLEDCSA---FALRYNGKSYAILRNPEFYEHRKEERCSRQFGTSNEGHPYVKMIYESGDWLVGGDLEVLERICWNDGLDKYRLTPNELRSKFKKMGADAVFAFQLRNPIHNGHALLMQQTRE 424
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A7M7KIY6_VARDE (Uncharacterized protein n=4 Tax=Varroa destructor TaxID=109461 RepID=A0A7M7KIY6_VARDE)

HSP 1 Score: 179 bits (454), Expect = 1.140e-50
Identity = 83/134 (61.94%), Postives = 103/134 (76.87%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADT 402
            S+PIVL VS E +E +T     +++ L + G  + VLR  E + HRKEERCARQFG T+ GHPY++ +M SGDWL GGDL +  R++WNDGLDKYRLTP +LR EF+ RGADTV+AFQLRNPIHNGHALLM +T
Sbjct:  297 SIPIVLPVSTEDKERLTT---NSAITLRYQGKPLAVLREPEFYSHRKEERCARQFGLTNSGHPYIKMIMESGDWLCGGDLEVLERLKWNDGLDKYRLTPKELRAEFSRRGADTVFAFQLRNPIHNGHALLMKET 427
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A3Q1IX50_ANATE (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2-like n=8 Tax=Anabas testudineus TaxID=64144 RepID=A0A3Q1IX50_ANATE)

HSP 1 Score: 179 bits (453), Expect = 1.250e-50
Identity = 84/136 (61.76%), Postives = 106/136 (77.94%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            SVPIVLAVS E ++ +      A+VAL + GS V ++RN E +EHRKEERCARQ+GTT   HPY++ VM SGDWLVGGDL +  R++WNDGLD+YRLTP QL+++F    AD V+AFQLRNP+HNGHALLM DT++
Sbjct:  282 SVPIVLAVSTETKQELNGC---AAVALEYQGSRVAIIRNPEFYEHRKEERCARQWGTTCPQHPYIKMVMESGDWLVGGDLEVLERIKWNDGLDQYRLTPRQLKQKFKEMKADAVFAFQLRNPVHNGHALLMQDTKR 414
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A1D1V6E1_RAMVA (Uncharacterized protein n=2 Tax=Ramazzottius varieornatus TaxID=947166 RepID=A0A1D1V6E1_RAMVA)

HSP 1 Score: 178 bits (452), Expect = 2.480e-50
Identity = 84/136 (61.76%), Postives = 107/136 (78.68%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            S+PIVLAVS E +E +    A   +AL +N   V +LR  E +EHRKEERCAR FGTT+ GHPY++ +M SGDWLVGG+L +  R++WNDGLD+YRLTPN+LR++F + GAD ++AFQLRNPIHNGHALLM DTR+
Sbjct:  311 SIPIVLAVSTEDKERLKDQQA---IALTYNQKPVAILRCPEFYEHRKEERCARTFGTTNDGHPYIKMIMESGDWLVGGELDVLERIRWNDGLDEYRLTPNELRKKFHTLGADAIFAFQLRNPIHNGHALLMQDTRR 443
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BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Match: A0A3B5LR34_9TELE (Sulfate adenylyltransferase n=1 Tax=Xiphophorus couchianus TaxID=32473 RepID=A0A3B5LR34_9TELE)

HSP 1 Score: 173 bits (438), Expect = 2.800e-50
Identity = 83/136 (61.03%), Postives = 103/136 (75.74%), Query Frame = 1 
Query:    1 SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEERCARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPNQLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK 408
            SVPIVL VS   +E +    A   +AL +NG  V +LRN E ++HRKEERCARQ+GTT   HPY++ VM SGDWLVGGDL +  R+ WNDGLD+YRLTPN+L+++F    AD V+AFQLRNP+HNGHALLM DT+K
Sbjct:   59 SVPIVLPVSTADKEHLDGVTA---MALVYNGRRVAILRNPEFYQHRKEERCARQWGTTCKDHPYIKMVMESGDWLVGGDLQVLDRIYWNDGLDQYRLTPNELKQKFKEMNADAVFAFQLRNPVHNGHALLMQDTQK 191
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig127931.5626.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI000C6ECD941.160e-5262.50bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
A0A1W0WTB0_HYPDU7.600e-5263.24Bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
A0A3B4CB60_PYGNA1.440e-5163.24Bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
A0A6A4WVU4_AMPAM3.230e-5161.76Sulfate adenylyltransferase n=1 Tax=Amphibalanus a... [more]
UPI00143A27C23.280e-5161.03bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
UPI000C6CD1045.300e-5162.50bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
A0A7M7KIY6_VARDE1.140e-5061.94Uncharacterized protein n=4 Tax=Varroa destructor ... [more]
A0A3Q1IX50_ANATE1.250e-5061.76Bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
A0A1D1V6E1_RAMVA2.480e-5061.76Uncharacterized protein n=2 Tax=Ramazzottius varie... [more]
A0A3B5LR34_9TELE2.800e-5061.03Sulfate adenylyltransferase n=1 Tax=Xiphophorus co... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig127931contigM-pyrifera_M_contig127931:2..412 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score177.9
Seed ortholog evalue3.1e-42
Seed eggNOG ortholog69293.ENSGACP00000012173
Preferred namePAPSS2
KEGG rclassRC00002,RC00078,RC02809,RC02889
KEGG koko:K13811
KEGG ReactionR00509,R00529,R04928,R04929
KEGG Pathwayko00230,ko00261,ko00450,ko00920,ko01100,ko01120,ko01130,map00230,map00261,map00450,map00920,map01100,map01120,map01130
KEGG ModuleM00176
Hectar predicted targeting categoryother localisation
GOsGO:0000103,GO:0001501,GO:0003674,GO:0003824,GO:0004020,GO:0004779,GO:0004781,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0006082,GO:0006139,GO:0006163,GO:0006164,GO:0006725,GO:0006753,GO:0006790,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0007275,GO:0007596,GO:0007599,GO:0008150,GO:0008152,GO:0009058,GO:0009117,GO:0009150,GO:0009152,GO:0009165,GO:0009259,GO:0009260,GO:0009611,GO:0009987,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0016779,GO:0018130,GO:0019438,GO:0019637,GO:0019693,GO:0032501,GO:0032502,GO:0033865,GO:0033866,GO:0033875,GO:0034030,GO:0034032,GO:0034033,GO:0034035,GO:0034036,GO:0034641,GO:0034654,GO:0042060,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044272,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046390,GO:0046483,GO:0048513,GO:0048731,GO:0048856,GO:0050427,GO:0050428,GO:0050817,GO:0050878,GO:0050896,GO:0055086,GO:0060348,GO:0065007,GO:0065008,GO:0070566,GO:0071704,GO:0072521,GO:0072522,GO:0090407,GO:1901135,GO:1901137,GO:1901293,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.3'-phosphoadenosine 5'-phosphosulfate synthase
EggNOG OGs38GMU@33154,3BDIX@33208,3CVIK@33213,485PB@7711,492SF@7742,49WCX@7898,COG0529@1,KOG4238@2759
EC2.7.1.25,2.7.7.4
COG Functional cat.F
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size411
Cds size411
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815793.4743848-CDS-M-pyrifera_M_contig127931:1..4121622815793.4743848-CDS-M-pyrifera_M_contig127931:1..412Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127931 2..412 +
1692277572.3529088-CDS-M-pyrifera_M_contig127931:1..4121692277572.3529088-CDS-M-pyrifera_M_contig127931:1..412Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127931 2..412 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig127931.5626.1prot_M-pyrifera_M_contig127931.5626.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig127931 2..412 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig127931.5626.1

>prot_M-pyrifera_M_contig127931.5626.1 ID=prot_M-pyrifera_M_contig127931.5626.1|Name=mRNA_M-pyrifera_M_contig127931.5626.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=137bp
SVPIVLAVSDEGRESVTAAGAGASVALHFNGSAVGVLRNVELFEHRKEER
CARQFGTTDVGHPYVEQVMSSGDWLVGGDLVLFRRVQWNDGLDKYRLTPN
QLRREFTSRGADTVYAFQLRNPIHNGHALLMADTRK*
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mRNA from alignment at M-pyrifera_M_contig127931:2..412+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig127931.5626.1 ID=mRNA_M-pyrifera_M_contig127931.5626.1|Name=mRNA_M-pyrifera_M_contig127931.5626.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=411bp|location=Sequence derived from alignment at M-pyrifera_M_contig127931:2..412+ (Macrocystis pyrifera P11B4 male)
TCCGTCCCCATCGTGCTGGCCGTGTCGGACGAAGGGCGCGAGAGCGTCAC
TGCCGCCGGCGCCGGTGCCTCGGTCGCGCTGCACTTCAACGGCAGCGCCG
TCGGCGTGCTGCGCAACGTGGAGCTGTTTGAGCACCGCAAGGAGGAGCGC
TGCGCGCGCCAGTTTGGCACCACGGACGTGGGCCACCCGTACGTGGAGCA
GGTCATGTCTTCCGGCGACTGGCTTGTGGGAGGCGACCTGGTGCTGTTCC
GTCGCGTCCAGTGGAACGACGGGCTGGACAAGTACCGCCTGACGCCCAAC
CAGCTGCGGCGCGAGTTCACCAGCCGCGGCGCGGACACCGTGTACGCGTT
CCAGCTGCGCAACCCGATCCACAACGGCCACGCGCTGCTGATGGCGGACA
CGAGGAAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig127931:2..412+

>mRNA_M-pyrifera_M_contig127931.5626.1 ID=mRNA_M-pyrifera_M_contig127931.5626.1|Name=mRNA_M-pyrifera_M_contig127931.5626.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=822bp|location=Sequence derived from alignment at M-pyrifera_M_contig127931:2..412+ (Macrocystis pyrifera P11B4 male)
TCCGTCCCCATCGTGCTGGCCGTGTCGGACGAAGGGCGCGAGAGCGTCAC
TGCCGCCGGCGCCGGTGCCTCGGTCGCGCTGCACTTCAACGGCAGCGCCG
TCGGCGTGCTGCGCAACGTGGAGCTGTTTGAGCACCGCAAGGAGGAGCGC
TGCGCGCGCCAGTTTGGCACCACGGACGTGGGCCACCCGTACGTGGAGCA
GGTCATGTCTTCCGGCGACTGGCTTGTGGGAGGCGACCTGGTGCTGTTCC
GTCGCGTCCAGTGGAACGACGGGCTGGACAAGTACCGCCTGACGCCCAAC
CAGCTGCGGCGCGAGTTCACCAGCCGCGGCGCGGACACCGTGTACGCGTT
CCAGCTGCGCAACCCGATCCACAACGGCCACGCGCTGCTGATGGCGGACA
CGAGGAAGTGATCCGTCCCCATCGTGCTGGCCGTGTCGGACGAAGGGCGC
GAGAGCGTCACTGCCGCCGGCGCCGGTGCCTCGGTCGCGCTGCACTTCAA
CGGCAGCGCCGTCGGCGTGCTGCGCAACGTGGAGCTGTTTGAGCACCGCA
AGGAGGAGCGCTGCGCGCGCCAGTTTGGCACCACGGACGTGGGCCACCCG
TACGTGGAGCAGGTCATGTCTTCCGGCGACTGGCTTGTGGGAGGCGACCT
GGTGCTGTTCCGTCGCGTCCAGTGGAACGACGGGCTGGACAAGTACCGCC
TGACGCCCAACCAGCTGCGGCGCGAGTTCACCAGCCGCGGCGCGGACACC
GTGTACGCGTTCCAGCTGCGCAACCCGATCCACAACGGCCACGCGCTGCT
GATGGCGGACACGAGGAAGTGA
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