mRNA_M-pyrifera_M_contig127792.5606.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig127792.5606.1
Unique NamemRNA_M-pyrifera_M_contig127792.5606.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A059LB20_9CHLO (Dus domain-containing protein (Fragment) n=1 Tax=Helicosporidium sp. ATCC 50920 TaxID=1291522 RepID=A0A059LB20_9CHLO)

HSP 1 Score: 95.9 bits (237), Expect = 1.090e-23
Identity = 42/84 (50.00%), Postives = 60/84 (71.43%), Query Frame = 1 
Query:   19 ELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMSTQM 270
            ++RGK V+APLTTVGN+PFRR+    GA+VT GEMA+A  L QG+  EW+  K HH E+CFG Q+    P+ AAR  ++++ ++
Sbjct:   21 DVRGKRVLAPLTTVGNLPFRRLCVALGAEVTYGEMALATGLLQGQPSEWALAKMHHSERCFGVQICAGFPDAAARAAQVVAERL 104
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: B8BWN9_THAPS (Dus domain-containing protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BWN9_THAPS)

HSP 1 Score: 99.4 bits (246), Expect = 8.200e-23
Identity = 47/88 (53.41%), Postives = 62/88 (70.45%), Query Frame = 1 
Query:    4 RKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMSTQ 267
            RK ++  R KV +APLTTVGN+PFRRI+ GFGAD+T GEMAIA QL QG+  EW+ LK H  E  FG Q+A    ++  R+ EL++ +
Sbjct:    6 RKLID-FRNKVYIAPLTTVGNLPFRRIMKGFGADITCGEMAIADQLLQGKPSEWALLKRHSSEDVFGVQIATGHADQYTRVAELIANE 92
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A0G4GH78_VITBC (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=2 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4GH78_VITBC)

HSP 1 Score: 100 bits (249), Expect = 9.440e-23
Identity = 46/80 (57.50%), Postives = 59/80 (73.75%), Query Frame = 1 
Query:   19 ELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELM 258
            +LRGK+V+APLTTVGN+PFRR+    GADVTVGEMA+A  L QG+  EW+ LK H +EKCFG Q+A   P+   R  E++
Sbjct:  270 DLRGKLVLAPLTTVGNLPFRRLCRRLGADVTVGEMALADSLLQGKPSEWALLKRHEEEKCFGVQVASGWPDVMTRCAEML 349
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A267GQQ1_9PLAT (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (Fragment) n=3 Tax=Macrostomum lignano TaxID=282301 RepID=A0A267GQQ1_9PLAT)

HSP 1 Score: 100 bits (248), Expect = 1.280e-22
Identity = 47/85 (55.29%), Postives = 63/85 (74.12%), Query Frame = 1 
Query:    4 RKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELM 258
            RK   + RGK+++APLTTVGN+PFRRI   FGADVT GEMA+A  L QGR  EW+ L+ H  E+CFGAQ+  + P++ AR+ +L+
Sbjct:  278 RKRRLDFRGKLLLAPLTTVGNLPFRRICVDFGADVTCGEMAMASNLLQGRNAEWALLRRHPSERCFGAQVCGSHPDQLARLAQLL 362
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A7K7DNI3_PHEME (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (Fragment) n=1 Tax=Pheucticus melanocephalus TaxID=371919 RepID=A0A7K7DNI3_PHEME)

HSP 1 Score: 95.9 bits (237), Expect = 2.930e-22
Identity = 44/81 (54.32%), Postives = 56/81 (69.14%), Query Frame = 1 
Query:   19 ELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMS 261
            E++GK+ +APLTT GN+PFRRI  GFGADVT GEMA+   L QG+  EW+ LK HH E  FG QL  A P+   +  EL++
Sbjct:  178 EIQGKLYLAPLTTCGNLPFRRICKGFGADVTCGEMAVCTNLLQGQSSEWALLKRHHTEDIFGVQLEGAFPDTMTKCAELLN 258
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A812U5K9_9DINO (DUS3L protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812U5K9_9DINO)

HSP 1 Score: 99.0 bits (245), Expect = 3.240e-22
Identity = 48/90 (53.33%), Postives = 64/90 (71.11%), Query Frame = 1 
Query:    1 RRKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMSTQM 270
            R K   +L+GKV +APLTTVGN+P+RRI+ G GADVTV EMA+A QL +G   EW+ +K H  EK FG Q+A A PE+  R+ EL+  ++
Sbjct:  215 REKRPLDLQGKVYIAPLTTVGNLPYRRIMVGLGADVTVSEMALAHQLLKGENREWALVKRHPCEKMFGLQVAGAHPEDMGRLAELVQNEL 304
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A852CHE2_9PICI (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (Fragment) n=1 Tax=Ramphastos sulfuratus TaxID=322582 RepID=A0A852CHE2_9PICI)

HSP 1 Score: 96.3 bits (238), Expect = 3.830e-22
Identity = 45/87 (51.72%), Postives = 58/87 (66.67%), Query Frame = 1 
Query:    1 RRKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMS 261
            R K   E++GK+ +APLTT GN+PFRR+  GFGADVT GEMA+   L QG+  EW+ LK HH E  FG QL  A P+   +  EL++
Sbjct:  185 REKRKLEIQGKLYLAPLTTCGNLPFRRVCKGFGADVTCGEMAVCTNLLQGQASEWALLKRHHTEDIFGVQLEGAFPDTMTKCAELLN 271
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BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A2K3DUZ8_CHLRE (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3DUZ8_CHLRE)

HSP 1 Score: 98.2 bits (243), Expect = 6.220e-22
Identity = 49/89 (55.06%), Postives = 61/89 (68.54%), Query Frame = 1 
Query:    1 RRKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMSTQ 267
            RRK   +LRGK  +APLTTVGN+PFRRIV G G DVT GEMA+A  L QG+  EW+ LK H  E  FG Q+    P+  AR+G+L+S +
Sbjct:  425 RRKL--DLRGKTYLAPLTTVGNLPFRRIVKGLGCDVTCGEMALATNLLQGQASEWALLKRHPCEDLFGVQVCGGYPDALARVGQLLSQE 511
[Close]
BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: A0A835WSZ8_9CHLO (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=2 Tax=Chlamydomonas schloesseri TaxID=2026947 RepID=A0A835WSZ8_9CHLO)

HSP 1 Score: 97.8 bits (242), Expect = 8.500e-22
Identity = 48/89 (53.93%), Postives = 61/89 (68.54%), Query Frame = 1 
Query:    1 RRKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMSTQ 267
            RRK   +LRGK  +APLTTVGN+PFRR+V G G DVT GEMA+A  L QG+  EW+ LK H  E  FG Q+    P+  AR+G+L+S +
Sbjct:  447 RRKL--DLRGKTYLAPLTTVGNLPFRRVVKGLGCDVTCGEMALATNLLQGQASEWALLKRHPCEDLFGVQVCGGYPDALARVGQLLSQE 533
[Close]
BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Match: F0YG32_AURAN (Dus domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YG32_AURAN)

HSP 1 Score: 96.3 bits (238), Expect = 1.020e-21
Identity = 45/85 (52.94%), Postives = 61/85 (71.76%), Query Frame = 1 
Query:    4 RKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQGREEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELM 258
            RK V+   GKV VAPLTTVGN+PFRR+++ +GAD+T GEMA+   LN G+  EW+ L+ H DEKCFG QL+ A  ++  R  E++
Sbjct:    2 RKLVD-FSGKVYVAPLTTVGNLPFRRVLTKYGADITCGEMALCPNLNDGQSSEWALLRRHKDEKCFGVQLSAAHGDQFGRACEVV 85
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig127792.5606.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A059LB20_9CHLO1.090e-2350.00Dus domain-containing protein (Fragment) n=1 Tax=H... [more]
B8BWN9_THAPS8.200e-2353.41Dus domain-containing protein (Fragment) n=1 Tax=T... [more]
A0A0G4GH78_VITBC9.440e-2357.50tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=2 T... [more]
A0A267GQQ1_9PLAT1.280e-2255.29tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (Frag... [more]
A0A7K7DNI3_PHEME2.930e-2254.32tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (Frag... [more]
A0A812U5K9_9DINO3.240e-2253.33DUS3L protein n=1 Tax=Symbiodinium sp. KB8 TaxID=2... [more]
A0A852CHE2_9PICI3.830e-2251.72tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (Frag... [more]
A0A2K3DUZ8_CHLRE6.220e-2255.06tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=1 T... [more]
A0A835WSZ8_9CHLO8.500e-2253.93tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=2 T... [more]
F0YG32_AURAN1.020e-2152.94Dus domain-containing protein (Fragment) n=1 Tax=A... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig127792contigM-pyrifera_M_contig127792:153..422 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score99.0
Seed ortholog evalue1.2e-18
Seed eggNOG ortholog35128.Thaps32996
Preferred namedus3l
KEGG koko:K05544
Hectar predicted targeting categoryother localisation
GOsGO:0002943,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009987,GO:0010467,GO:0016070,GO:0016491,GO:0016627,GO:0017150,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0055114,GO:0071704,GO:0090304,GO:0140098,GO:0140101,GO:1901360
EggNOG free text desc.tRNA dihydrouridine synthase activity
EggNOG OGs2XAZ2@2836,COG0042@1,KOG2333@2759
EC1.3.1.89
COG Functional cat.J
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Exons1
Model size270
Cds size270
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815792.436221-CDS-M-pyrifera_M_contig127792:152..4221622815792.436221-CDS-M-pyrifera_M_contig127792:152..422Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127792 153..422 -
1692277571.821915-CDS-M-pyrifera_M_contig127792:152..4221692277571.821915-CDS-M-pyrifera_M_contig127792:152..422Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127792 153..422 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig127792.5606.1prot_M-pyrifera_M_contig127792.5606.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig127792 153..422 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig127792.5606.1

>prot_M-pyrifera_M_contig127792.5606.1 ID=prot_M-pyrifera_M_contig127792.5606.1|Name=mRNA_M-pyrifera_M_contig127792.5606.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=90bp
RRKFVEELRGKVVVAPLTTVGNVPFRRIVSGFGADVTVGEMAIAKQLNQG
REEEWSFLKAHHDEKCFGAQLAIALPEEAARIGELMSTQM
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mRNA from alignment at M-pyrifera_M_contig127792:153..422-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig127792.5606.1 ID=mRNA_M-pyrifera_M_contig127792.5606.1|Name=mRNA_M-pyrifera_M_contig127792.5606.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=270bp|location=Sequence derived from alignment at M-pyrifera_M_contig127792:153..422- (Macrocystis pyrifera P11B4 male)
CGCCGCAAGTTTGTGGAGGAGTTGCGAGGGAAGGTGGTGGTGGCTCCGTT
GACAACGGTGGGCAATGTTCCTTTTCGAAGAATCGTGAGTGGGTTTGGGG
CTGATGTCACTGTTGGGGAAATGGCGATAGCGAAGCAACTGAACCAAGGG
AGAGAAGAAGAGTGGAGCTTTCTGAAAGCACACCACGATGAAAAGTGCTT
TGGAGCACAACTTGCCATTGCTCTCCCTGAAGAAGCAGCAAGAATCGGAG
AACTAATGTCCACACAAATG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig127792:153..422-

>mRNA_M-pyrifera_M_contig127792.5606.1 ID=mRNA_M-pyrifera_M_contig127792.5606.1|Name=mRNA_M-pyrifera_M_contig127792.5606.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=540bp|location=Sequence derived from alignment at M-pyrifera_M_contig127792:153..422- (Macrocystis pyrifera P11B4 male)
CGCCGCAAGTTTGTGGAGGAGTTGCGAGGGAAGGTGGTGGTGGCTCCGTT
GACAACGGTGGGCAATGTTCCTTTTCGAAGAATCGTGAGTGGGTTTGGGG
CTGATGTCACTGTTGGGGAAATGGCGATAGCGAAGCAACTGAACCAAGGG
AGAGAAGAAGAGTGGAGCTTTCTGAAAGCACACCACGATGAAAAGTGCTT
TGGAGCACAACTTGCCATTGCTCTCCCTGAAGAAGCAGCAAGAATCGGAG
AACTAATGTCCACACAAATGCGCCGCAAGTTTGTGGAGGAGTTGCGAGGG
AAGGTGGTGGTGGCTCCGTTGACAACGGTGGGCAATGTTCCTTTTCGAAG
AATCGTGAGTGGGTTTGGGGCTGATGTCACTGTTGGGGAAATGGCGATAG
CGAAGCAACTGAACCAAGGGAGAGAAGAAGAGTGGAGCTTTCTGAAAGCA
CACCACGATGAAAAGTGCTTTGGAGCACAACTTGCCATTGCTCTCCCTGA
AGAAGCAGCAAGAATCGGAGAACTAATGTCCACACAAATG
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