mRNA_M-pyrifera_M_contig126902.5417.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig126902.5417.1
Unique NamemRNA_M-pyrifera_M_contig126902.5417.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: A0A1Y1I813_KLENI (2-amino-3-ketobutyrate coenzyme A ligase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1I813_KLENI)

HSP 1 Score: 193 bits (490), Expect = 1.760e-57
Identity = 93/125 (74.40%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            VISDELNHASIIDG+RLCKA+R RYKH+DM +LE  L   + + V+L+ TDGVFSMDG VAPLD+IV L  KH AV  VD+CHATGF GA GRGTDEYCGV G+VD+INSTLGKALGGATGGYTT
Sbjct:  184 VISDELNHASIIDGIRLCKAQRRRYKHVDMADLETHLRETQNARVRLIATDGVFSMDGHVAPLDEIVGLAQKHDAVTFVDDCHATGFLGATGRGTDEYCGVQGKVDIINSTLGKALGGATGGYTT 308
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: A0A6J1SRL5_FRAOC (2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial n=1 Tax=Frankliniella occidentalis TaxID=133901 RepID=A0A6J1SRL5_FRAOC)

HSP 1 Score: 191 bits (484), Expect = 6.940e-57
Identity = 89/125 (71.20%), Postives = 108/125 (86.40%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            ++SDELNHAS+IDG+RLCKAK+ RYKH DME+LE  L  N +S +KL+VTDGVFSMDG VAPL +IV L +++GA+ L+DECHATGFFG+ GRGT+EY GV G+VD+INSTLGKALGGA GGYTT
Sbjct:  154 ILSDELNHASLIDGIRLCKAKKNRYKHRDMEDLERQLKENSSSRIKLIVTDGVFSMDGTVAPLPQIVELAERYGALTLIDECHATGFFGSTGRGTEEYHGVSGKVDIINSTLGKALGGAAGGYTT 278
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: A0A5A8EQX0_CAFRO (Aminotran_1_2 domain-containing protein n=6 Tax=Sar TaxID=2698737 RepID=A0A5A8EQX0_CAFRO)

HSP 1 Score: 189 bits (481), Expect = 1.860e-56
Identity = 90/124 (72.58%), Postives = 103/124 (83.06%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYT 372
            +ISDELNHASIIDGVRLCKA R RYKH+DM +LE  L+ +  SPV+L+ TDG FSMDGDVAPLD+I  L  KH A L +DECHATGFFG  GRGTDE+ G+ G+VD+INSTLGKALGGATGGYT
Sbjct:  151 IISDELNHASIIDGVRLCKANRYRYKHMDMGDLERCLVESADSPVRLIATDGAFSMDGDVAPLDEIHALAQKHDAKLFIDECHATGFFGPTGRGTDEFFGLHGKVDIINSTLGKALGGATGGYT 274
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: UPI000D74003B (LOW QUALITY PROTEIN: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial-like n=1 Tax=Pomacea canaliculata TaxID=400727 RepID=UPI000D74003B)

HSP 1 Score: 187 bits (475), Expect = 1.760e-55
Identity = 87/125 (69.60%), Postives = 107/125 (85.60%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            +ISDELNHASIIDG+RLCKA+R+RYKH DME+LE  L  ++ +  +L+ TDGVFSMDG+VAPLDKI +L DK+ A++ +DECHATGFFGA GRGT+EY  + G+VDVINSTLGKA+GGA GGYTT
Sbjct:  164 IISDELNHASIIDGIRLCKAQRQRYKHRDMEDLENNLKNSQGNRQRLIATDGVFSMDGNVAPLDKICSLADKYNALVFIDECHATGFFGATGRGTEEYLRIQGKVDVINSTLGKAMGGAAGGYTT 288
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: A0A1V9YYV9_9STRA (2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9YYV9_9STRA)

HSP 1 Score: 186 bits (472), Expect = 2.160e-55
Identity = 86/125 (68.80%), Postives = 103/125 (82.40%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            VISDELNHASIIDG+RLCKA+R R+KH+D+E+LE+ L+  +    +L+ TDG FSMDGDVAPL  I  L DK+ AV+ +DECHATGFFGA GRG+DEYC V G +D+INSTLGKALGG TGGYTT
Sbjct:  126 VISDELNHASIIDGIRLCKAQRHRFKHMDLEDLEKQLIATQHCRTRLIATDGAFSMDGDVAPLKGICDLADKYNAVVFIDECHATGFFGATGRGSDEYCNVRGRIDIINSTLGKALGGGTGGYTT 250
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: V4AZZ4_LOTGI (Aminotran_1_2 domain-containing protein n=1 Tax=Lottia gigantea TaxID=225164 RepID=V4AZZ4_LOTGI)

HSP 1 Score: 187 bits (474), Expect = 2.650e-55
Identity = 87/124 (70.16%), Postives = 105/124 (84.68%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYT 372
            VISDELNHASIIDG+RLCKA+R RYKH DM +LE +L++ + S  +L+VTDGVFSMDG+VAPL +I  L DKH A++ +DECHATGFFG  GRGT+E+CG+ G VD+INSTLGKALGGA GGYT
Sbjct:  162 VISDELNHASIIDGIRLCKAERLRYKHRDMADLENLLIKTKGSRQRLIVTDGVFSMDGNVAPLKEICDLADKHKALVFIDECHATGFFGKTGRGTEEHCGLTGRVDIINSTLGKALGGAAGGYT 285
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: UPI001F04B1BE (2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial-like n=1 Tax=Xenia sp. Carnegie-2017 TaxID=2897299 RepID=UPI001F04B1BE)

HSP 1 Score: 186 bits (473), Expect = 3.490e-55
Identity = 89/125 (71.20%), Postives = 104/125 (83.20%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            V SDELNHASIIDGVRLCKAK+ RYKH DM +LE +L  NE + VKLV+TDGVFSMDG+VAPL +I  L DK+GA++ +DECHATGFFG  GRGT+E+  + G VD+INSTLGKALGGA GGYTT
Sbjct:  160 VFSDELNHASIIDGVRLCKAKKLRYKHKDMNDLERVLKENEEARVKLVITDGVFSMDGNVAPLREIAFLADKYGALIFIDECHATGFFGETGRGTEEFFSLTGRVDIINSTLGKALGGAAGGYTT 284
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: A0A2D4C997_PYTIN (2-amino-3-ketobutyrate coenzyme A ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C997_PYTIN)

HSP 1 Score: 186 bits (472), Expect = 4.000e-55
Identity = 87/125 (69.60%), Postives = 103/125 (82.40%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            +I+DELNHASIIDGVRLCKA+R R+KH+D+ +LE  L   +    +L+VTDGVFSMDGDVAPL +I  L DK+ A + VDECHATGFFGA GRG+DEYCGV G +D+INSTLGKALGG TGGYTT
Sbjct:  151 IITDELNHASIIDGVRLCKAERHRFKHMDLTDLEAKLQATQHCRTRLIVTDGVFSMDGDVAPLQEICDLADKYNAQIFVDECHATGFFGATGRGSDEYCGVQGRIDIINSTLGKALGGGTGGYTT 275
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: A0A0W8DAH3_PHYNI (Aminotran_1_2 domain-containing protein n=13 Tax=Peronosporaceae TaxID=4777 RepID=A0A0W8DAH3_PHYNI)

HSP 1 Score: 185 bits (470), Expect = 6.010e-55
Identity = 85/125 (68.00%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            +++DELNHASIIDG+RLCKA+R R+KH+DM +LE+ L   +    +L+ TDGVFSMDGDVAPL +IV L +K+ A L +DECHATGFFG  GRG+DEYCG+ G+VDVINSTLGKALGG+TGGYTT
Sbjct:  145 ILTDELNHASIIDGIRLCKAERHRFKHMDMTDLEQKLKDTQHCRTRLIATDGVFSMDGDVAPLQEIVALAEKYDAQLFIDECHATGFFGPTGRGSDEYCGITGKVDVINSTLGKALGGSTGGYTT 269
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BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Match: H3GAT3_PHYRM (Aminotran_1_2 domain-containing protein n=8 Tax=Peronosporaceae TaxID=4777 RepID=H3GAT3_PHYRM)

HSP 1 Score: 185 bits (469), Expect = 7.900e-55
Identity = 86/125 (68.80%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVTDGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCGVWGEVDVINSTLGKALGGATGGYTT 375
            +++DELNHASIIDG+RLCKA+R R+KH+DM++LE+ L   +    +L+ TDGVFSMDGDVAPL +IV L  K+ A L +DECHATGFFG  GRG+DEYCGV G+VDVINSTLGKALGG+TGGYTT
Sbjct:  135 ILTDELNHASIIDGIRLCKAERHRFKHMDMQDLEQKLKDTQHCRTRLIATDGVFSMDGDVAPLQEIVALAHKYDAQLFIDECHATGFFGPTGRGSDEYCGVNGKVDVINSTLGKALGGSTGGYTT 259
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig126902.5417.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1Y1I813_KLENI1.760e-5774.402-amino-3-ketobutyrate coenzyme A ligase n=1 Tax=K... [more]
A0A6J1SRL5_FRAOC6.940e-5771.202-amino-3-ketobutyrate coenzyme A ligase, mitochon... [more]
A0A5A8EQX0_CAFRO1.860e-5672.58Aminotran_1_2 domain-containing protein n=6 Tax=Sa... [more]
UPI000D74003B1.760e-5569.60LOW QUALITY PROTEIN: 2-amino-3-ketobutyrate coenzy... [more]
A0A1V9YYV9_9STRA2.160e-5568.802-amino-3-ketobutyrate coenzyme A ligase, mitochon... [more]
V4AZZ4_LOTGI2.650e-5570.16Aminotran_1_2 domain-containing protein n=1 Tax=Lo... [more]
UPI001F04B1BE3.490e-5571.202-amino-3-ketobutyrate coenzyme A ligase, mitochon... [more]
A0A2D4C997_PYTIN4.000e-5569.602-amino-3-ketobutyrate coenzyme A ligase n=1 Tax=P... [more]
A0A0W8DAH3_PHYNI6.010e-5568.00Aminotran_1_2 domain-containing protein n=13 Tax=P... [more]
H3GAT3_PHYRM7.900e-5568.80Aminotran_1_2 domain-containing protein n=8 Tax=Pe... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig126902contigM-pyrifera_M_contig126902:3..503 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score188.0
Seed ortholog evalue2.7e-45
Seed eggNOG ortholog42099.EPrPV00000020662
Preferred nameGCAT
KEGG rclassRC00004,RC00039,RC00394,RC02725
KEGG koko:K00639,ko:K00652,ko:K01176
KEGG ReactionR00371,R02108,R02112,R03210,R10124,R11262
KEGG Pathwayko00260,ko00500,ko00780,ko01100,ko04973,map00260,map00500,map00780,map01100,map04973
KEGG ModuleM00123,M00573,M00577
Hectar predicted targeting categoryother localisation
GOsGO:0001653,GO:0003674,GO:0003824,GO:0004888,GO:0004930,GO:0004966,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005886,GO:0005887,GO:0005929,GO:0006082,GO:0006355,GO:0006357,GO:0006520,GO:0006807,GO:0007154,GO:0007165,GO:0007186,GO:0007187,GO:0007188,GO:0007200,GO:0007218,GO:0008150,GO:0008152,GO:0008188,GO:0008528,GO:0008890,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0016020,GO:0016021,GO:0016407,GO:0016408,GO:0016453,GO:0016604,GO:0016607,GO:0016740,GO:0016746,GO:0016747,GO:0017046,GO:0019219,GO:0019222,GO:0019752,GO:0019866,GO:0023052,GO:0031090,GO:0031224,GO:0031226,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031966,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0033218,GO:0038023,GO:0042277,GO:0042562,GO:0042995,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0044237,GO:0044238,GO:0044281,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044429,GO:0044444,GO:0044446,GO:0044451,GO:0044459,GO:0044464,GO:0045893,GO:0045935,GO:0045944,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0051716,GO:0060089,GO:0060255,GO:0065007,GO:0070013,GO:0071704,GO:0071944,GO:0080090,GO:0090663,GO:0097730,GO:0120025,GO:1901564,GO:1902680,GO:1903506,GO:1903508,GO:2000112,GO:2001141
EggNOG free text desc.2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial. Source PGD
EggNOG OGs1MEZM@121069,COG0156@1,KOG1359@2759
EC2.3.1.29,2.3.1.47,3.2.1.1
COG Functional cat.E
CAZyGH13
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007
Exons2
Model size375
Cds size375
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815782.8983662-CDS-M-pyrifera_M_contig126902:2..1351622815782.8983662-CDS-M-pyrifera_M_contig126902:2..135Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig126902 3..135 -
1692277567.2132301-CDS-M-pyrifera_M_contig126902:2..1351692277567.2132301-CDS-M-pyrifera_M_contig126902:2..135Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig126902 3..135 -
1622815782.9089558-CDS-M-pyrifera_M_contig126902:261..5031622815782.9089558-CDS-M-pyrifera_M_contig126902:261..503Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig126902 262..503 -
1692277567.2250292-CDS-M-pyrifera_M_contig126902:261..5031692277567.2250292-CDS-M-pyrifera_M_contig126902:261..503Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig126902 262..503 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig126902.5417.1prot_M-pyrifera_M_contig126902.5417.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig126902 3..503 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig126902.5417.1

>prot_M-pyrifera_M_contig126902.5417.1 ID=prot_M-pyrifera_M_contig126902.5417.1|Name=mRNA_M-pyrifera_M_contig126902.5417.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=125bp
VISDELNHASIIDGVRLCKAKRERYKHLDMEELEEILLRNEASPVKLVVT
DGVFSMDGDVAPLDKIVTLCDKHGAVLLVDECHATGFFGARGRGTDEYCG
VWGEVDVINSTLGKALGGATGGYTT
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mRNA from alignment at M-pyrifera_M_contig126902:3..503-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig126902.5417.1 ID=mRNA_M-pyrifera_M_contig126902.5417.1|Name=mRNA_M-pyrifera_M_contig126902.5417.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=501bp|location=Sequence derived from alignment at M-pyrifera_M_contig126902:3..503- (Macrocystis pyrifera P11B4 male)
GTGATTAGTGATGAGTTGAACCATGCCTCCATCATTGATGGCGTTCGTCT
GTGCAAGGCGAAACGCGAACGATACAAGCACCTCGACATGGAAGAACTCG
AAGAAATCCTTCTCCGCAACGAAGCAAGTCCTGTCAAACTCGTTGTCACA
GATGGTGTGTTTTCCATGGATGGTGACGTAGCACCACTCGACAAGATTGT
CACACTCTGCGACAAACACGGTGCCGTACTCCTTGTAGATGAGTAAGAGA
AAGAGAGTGGGAGAGAGAGAGAGAGGTGGTTTGGTTTAGGACCTTTTTTC
GCCGTACCATTCTTCAACCCCTCCCTCACTTCTTTCTTCCCCACGTATTC
ATATTTCTCGTTTCACAGGTGTCACGCAACTGGATTTTTCGGAGCTCGTG
GTCGTGGAACAGATGAGTATTGTGGAGTGTGGGGGGAGGTTGATGTGATT
AACAGTACTTTGGGGAAGGCATTGGGTGGAGCCACTGGTGGGTATACTAC
A
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig126902:3..503-

>mRNA_M-pyrifera_M_contig126902.5417.1 ID=mRNA_M-pyrifera_M_contig126902.5417.1|Name=mRNA_M-pyrifera_M_contig126902.5417.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=750bp|location=Sequence derived from alignment at M-pyrifera_M_contig126902:3..503- (Macrocystis pyrifera P11B4 male)
GTGATTAGTGATGAGTTGAACCATGCCTCCATCATTGATGGCGTTCGTCT
GTGCAAGGCGAAACGCGAACGATACAAGCACCTCGACATGGAAGAACTCG
AAGAAATCCTTCTCCGCAACGAAGCAAGTCCTGTCAAACTCGTTGTCACA
GATGGTGTGTTTTCCATGGATGGTGACGTAGCACCACTCGACAAGATTGT
CACACTCTGCGACAAACACGGTGCCGTACTCCTTGTAGATGAGTGATTAG
TGATGAGTTGAACCATGCCTCCATCATTGATGGCGTTCGTCTGTGCAAGG
CGAAACGCGAACGATACAAGCACCTCGACATGGAAGAACTCGAAGAAATC
CTTCTCCGCAACGAAGCAAGTCCTGTCAAACTCGTTGTCACAGATGGTGT
GTTTTCCATGGATGGTGACGTAGCACCACTCGACAAGATTGTCACACTCT
GCGACAAACACGGTGCCGTACTCCTTGTAGATGAGTGTCACGCAACTGGA
TTTTTCGGAGCTCGTGGTCGTGGAACAGATGAGTATTGTGGAGTGTGGGG
GGAGGTTGATGTGATTAACAGTACTTTGGGGAAGGCATTGGGTGGAGCCA
CTGGTGGGTATACTACAGTGTCACGCAACTGGATTTTTCGGAGCTCGTGG
TCGTGGAACAGATGAGTATTGTGGAGTGTGGGGGGAGGTTGATGTGATTA
ACAGTACTTTGGGGAAGGCATTGGGTGGAGCCACTGGTGGGTATACTACA
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