mRNA_M-pyrifera_M_contig125766.5208.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125766.5208.1
Unique NamemRNA_M-pyrifera_M_contig125766.5208.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A517PL23_9PLAN (Macrolide export protein MacA n=2 Tax=Gimesia TaxID=1649453 RepID=A0A517PL23_9PLAN)

HSP 1 Score: 189 bits (479), Expect = 4.320e-56
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1 
Query:    1 MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKXXXXXXXXXXXXXXXXXXXXXXXXSPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKXXXXXXXXXXXXXXXXXXXXXXXXSPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG
Sbjct:    1 MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKXXXXXXXXXXXXXXXXXXXXXXXXSPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 122
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A518A438_9PLAN (Macrolide export protein MacA n=3 Tax=Gimesia panareensis TaxID=2527978 RepID=A0A518A438_9PLAN)

HSP 1 Score: 151 bits (381), Expect = 1.490e-41
Identity = 82/124 (66.13%), Postives = 87/124 (70.16%), Query Frame = 1 
Query:    1 MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKXXXXXXXXXXXXXXXXXXXXXXXX--SPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            M LLKETPVFLIGCLLTG+IFL W+PE+K  S+SHS K                          SP AVKRLGIETAPVEVR+MPRTR YGAEMVLPTGAAVIVSAPLAGTL HP  H FPQVG
Sbjct:    1 MNLLKETPVFLIGCLLTGVIFLLWSPEQKAPSESHSAKSKHGHGSEHSQTSKTLSEADLNVITLSPTAVKRLGIETAPVEVREMPRTRAYGAEMVLPTGAAVIVSAPLAGTLRHPQGHPFPQVG 124
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A517VEU1_9PLAN (Macrolide export protein MacA n=3 Tax=Planctomycetaceae TaxID=126 RepID=A0A517VEU1_9PLAN)

HSP 1 Score: 113 bits (282), Expect = 3.680e-27
Identity = 62/122 (50.82%), Postives = 74/122 (60.66%), Query Frame = 1 
Query:    1 MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKXXXXXXXXXXXXXXXXXXXXXXXXSPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            M++LK+T   L  CL+ GL+F+ W  ++K +  S S K                        SP AVK LG+ET PVE+R MPRTR YGAEMVLPTGA+VIVSAPLAGTL HP    FPQVG
Sbjct:    7 MKILKQTLALLGFCLILGLLFIIWDRDRKAQQKSTSTKTKHVEHHAKTTHPVNEADLNIIQLSPEAVKSLGVETQPVEIRSMPRTRPYGAEMVLPTGASVIVSAPLAGTLRHPHGDLFPQVG 128
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A350K509_9PLAN (Efflux transporter periplasmic adaptor subunit n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A350K509_9PLAN)

HSP 1 Score: 89.7 bits (221), Expect = 1.380e-18
Identity = 45/60 (75.00%), Postives = 49/60 (81.67%), Query Frame = 1 
Query:  187 SPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            SP AVK LGIET P+E+R MPR R YGAE+VLPTGA+VIVSAPLAGTL HP    FPQVG
Sbjct:   63 SPEAVKNLGIETEPLEMRSMPRMRPYGAELVLPTGASVIVSAPLAGTLRHPQGEQFPQVG 122
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A5C6B092_9PLAN (Macrolide transporter subunit MacA n=1 Tax=Symmachiella macrocystis TaxID=2527985 RepID=A0A5C6B092_9PLAN)

HSP 1 Score: 84.7 bits (208), Expect = 8.670e-17
Identity = 48/122 (39.34%), Postives = 64/122 (52.46%), Query Frame = 1 
Query:    1 MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKXXXXXXXXXXXXXXXXXXXXXXXXSPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            M +LK   +     ++  L+++ W   ++  S +   K                        SP AVKRL +E A +EVR MPR+R YGAE+VLPT A+VIVSAPLAGTL   P   FP+VG
Sbjct:    1 MSILKNLFIAASSIVIVALLYVLWTETQQKSSKAAPSKSAPAIAHSKTTHPVHESDLNIIELSPEAVKRLALEFATLEVRSMPRSRPYGAEVVLPTDASVIVSAPLAGTLRESPGREFPEVG 122
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A3L7NRY2_9BACT (HlyD family efflux transporter periplasmic adaptor subunit n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3L7NRY2_9BACT)

HSP 1 Score: 72.0 bits (175), Expect = 2.770e-12
Identity = 35/46 (76.09%), Postives = 40/46 (86.96%), Query Frame = 1 
Query:  196 AVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLN 333
            AV+RLGIET P+E R MPR RTYGAE+VLP GA+VIVSAPLAGT+ 
Sbjct:   52 AVRRLGIETQPIEERSMPRFRTYGAEVVLPAGASVIVSAPLAGTVK 97
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: UPI0019CECB5D (Efflux RND transporter periplasmic adaptor subunit n=1 Tax=Fuerstiella sp. TaxID=1936112 RepID=UPI0019CECB5D)

HSP 1 Score: 72.0 bits (175), Expect = 2.820e-12
Identity = 35/57 (61.40%), Postives = 44/57 (77.19%), Query Frame = 1 
Query:  196 AVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            AV+RLG++T  V +R MPR R YGAE++LP GA++IVSAPLAGTL      AFPQ+G
Sbjct:   63 AVRRLGLQTEVVTMRAMPRVRVYGAELMLPAGASIIVSAPLAGTLQLQTGQAFPQIG 119
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BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A0A2S8GQX3_9BACT (Efflux transporter periplasmic adaptor subunit n=1 Tax=Blastopirellula marina TaxID=124 RepID=A0A2S8GQX3_9BACT)

HSP 1 Score: 67.0 bits (162), Expect = 1.630e-10
Identity = 34/57 (59.65%), Postives = 45/57 (78.95%), Query Frame = 1 
Query:  196 AVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            A++RLG+ET  V+ R+M R+R YGA++VLPT AAVIVSAPLAGTL+ P +    +VG
Sbjct:   62 AIRRLGLETQEVKEREMYRSRPYGADLVLPTDAAVIVSAPLAGTLHSPSDQPRLEVG 118
[Close]
BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: A3ZX58_9BACT (Possible membrane fusion protein MtrC n=2 Tax=Blastopirellula marina TaxID=124 RepID=A3ZX58_9BACT)

HSP 1 Score: 66.6 bits (161), Expect = 2.230e-10
Identity = 33/48 (68.75%), Postives = 39/48 (81.25%), Query Frame = 1 
Query:  196 AVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHP 339
            A++RLG+ET  VE R M RTR YGA++VLPT A+VIVSAPLAGTL  P
Sbjct:   62 AIRRLGLETVAVEKRKMVRTRPYGADLVLPTDASVIVSAPLAGTLRSP 109
[Close]
BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Match: UPI0019C0354D (Efflux RND transporter periplasmic adaptor subunit n=1 Tax=Candidatus Saccharimonas sp. TaxID=1917697 RepID=UPI0019C0354D)

HSP 1 Score: 65.5 bits (158), Expect = 5.630e-10
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 1 
Query:  196 AVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAVIVSAPLAGTLNHPPEHAFPQVG 366
            A +RLGI+TA VE+R + RTRTYG E+ LPTGA +IVSAPL+GTL    +   P VG
Sbjct:   52 AEERLGIKTAAVEIRPVRRTRTYGGEVTLPTGALIIVSAPLSGTLQAAGKGPVPGVG 108
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125766.5208.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
A0A517PL23_9PLAN4.320e-56100.00Macrolide export protein MacA n=2 Tax=Gimesia TaxI... [more]
A0A518A438_9PLAN1.490e-4166.13Macrolide export protein MacA n=3 Tax=Gimesia pana... [more]
A0A517VEU1_9PLAN3.680e-2750.82Macrolide export protein MacA n=3 Tax=Planctomycet... [more]
A0A350K509_9PLAN1.380e-1875.00Efflux transporter periplasmic adaptor subunit n=1... [more]
A0A5C6B092_9PLAN8.670e-1739.34Macrolide transporter subunit MacA n=1 Tax=Symmach... [more]
A0A3L7NRY2_9BACT2.770e-1276.09HlyD family efflux transporter periplasmic adaptor... [more]
UPI0019CECB5D2.820e-1261.40Efflux RND transporter periplasmic adaptor subunit... [more]
A0A2S8GQX3_9BACT1.630e-1059.65Efflux transporter periplasmic adaptor subunit n=1... [more]
A3ZX58_9BACT2.230e-1068.75Possible membrane fusion protein MtrC n=2 Tax=Blas... [more]
UPI0019C0354D5.630e-1059.65Efflux RND transporter periplasmic adaptor subunit... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125766contigM-pyrifera_M_contig125766:2..367 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score67.8
Seed ortholog evalue4e-09
Seed eggNOG ortholog314230.DSM3645_27703
Hectar predicted targeting categorysignal anchor
EggNOG free text desc.Barrel-sandwich domain of CusB or HlyD membrane-fusion
EggNOG OGs2IWUN@203682,COG0845@1,COG0845@2
COG Functional cat.M
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
Exons1
Model size366
Cds size366
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815773.272489-CDS-M-pyrifera_M_contig125766:1..3671622815773.272489-CDS-M-pyrifera_M_contig125766:1..367Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125766 2..367 -
1692277562.6591747-CDS-M-pyrifera_M_contig125766:1..3671692277562.6591747-CDS-M-pyrifera_M_contig125766:1..367Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125766 2..367 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125766.5208.1prot_M-pyrifera_M_contig125766.5208.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125766 2..367 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125766.5208.1

>prot_M-pyrifera_M_contig125766.5208.1 ID=prot_M-pyrifera_M_contig125766.5208.1|Name=mRNA_M-pyrifera_M_contig125766.5208.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=122bp
MQLLKETPVFLIGCLLTGLIFLFWAPEKKNKSDSHSGKSHHGSKHSQTSK
TLNEADLNIITLSPIAVKRLGIETAPVEVRDMPRTRTYGAEMVLPTGAAV
IVSAPLAGTLNHPPEHAFPQVG
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mRNA from alignment at M-pyrifera_M_contig125766:2..367-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125766.5208.1 ID=mRNA_M-pyrifera_M_contig125766.5208.1|Name=mRNA_M-pyrifera_M_contig125766.5208.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=366bp|location=Sequence derived from alignment at M-pyrifera_M_contig125766:2..367- (Macrocystis pyrifera P11B4 male)
ATGCAGTTATTGAAAGAAACACCTGTGTTCCTGATAGGCTGTCTGCTGAC
CGGACTGATTTTCCTGTTCTGGGCTCCGGAGAAAAAGAACAAATCAGATT
CCCACTCAGGCAAATCACACCATGGATCAAAACACTCCCAAACCAGCAAG
ACGTTGAACGAAGCCGACCTGAACATCATCACCCTCAGCCCGATCGCAGT
CAAACGTCTGGGAATTGAAACGGCCCCCGTTGAAGTACGCGACATGCCCC
GCACCCGTACGTATGGAGCCGAGATGGTGCTCCCCACCGGCGCAGCGGTC
ATTGTCTCCGCTCCCCTGGCAGGTACTCTAAATCATCCCCCCGAACATGC
CTTTCCCCAGGTCGGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125766:2..367-

>mRNA_M-pyrifera_M_contig125766.5208.1 ID=mRNA_M-pyrifera_M_contig125766.5208.1|Name=mRNA_M-pyrifera_M_contig125766.5208.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=732bp|location=Sequence derived from alignment at M-pyrifera_M_contig125766:2..367- (Macrocystis pyrifera P11B4 male)
ATGCAGTTATTGAAAGAAACACCTGTGTTCCTGATAGGCTGTCTGCTGAC
CGGACTGATTTTCCTGTTCTGGGCTCCGGAGAAAAAGAACAAATCAGATT
CCCACTCAGGCAAATCACACCATGGATCAAAACACTCCCAAACCAGCAAG
ACGTTGAACGAAGCCGACCTGAACATCATCACCCTCAGCCCGATCGCAGT
CAAACGTCTGGGAATTGAAACGGCCCCCGTTGAAGTACGCGACATGCCCC
GCACCCGTACGTATGGAGCCGAGATGGTGCTCCCCACCGGCGCAGCGGTC
ATTGTCTCCGCTCCCCTGGCAGGTACTCTAAATCATCCCCCCGAACATGC
CTTTCCCCAGGTCGGAATGCAGTTATTGAAAGAAACACCTGTGTTCCTGA
TAGGCTGTCTGCTGACCGGACTGATTTTCCTGTTCTGGGCTCCGGAGAAA
AAGAACAAATCAGATTCCCACTCAGGCAAATCACACCATGGATCAAAACA
CTCCCAAACCAGCAAGACGTTGAACGAAGCCGACCTGAACATCATCACCC
TCAGCCCGATCGCAGTCAAACGTCTGGGAATTGAAACGGCCCCCGTTGAA
GTACGCGACATGCCCCGCACCCGTACGTATGGAGCCGAGATGGTGCTCCC
CACCGGCGCAGCGGTCATTGTCTCCGCTCCCCTGGCAGGTACTCTAAATC
ATCCCCCCGAACATGCCTTTCCCCAGGTCGGA
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