mRNA_M-pyrifera_M_contig125581.5177.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125581.5177.1
Unique NamemRNA_M-pyrifera_M_contig125581.5177.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: K8YZQ6_NANGC (Protein kinase domain-containing protein n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=K8YZQ6_NANGC)

HSP 1 Score: 179 bits (454), Expect = 1.330e-51
Identity = 86/158 (54.43%), Postives = 109/158 (68.99%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARG 492
            VRLL     + H+ +VFE+LEM++R+ L KFG+ VGL + AVR Y + L  AL  +  LG+VHAD+KPDN+LV    G+  L DFGSAFR D PDN+PT  L+SRFYR PEV LGF+    VDLWS  C L+ELFTG+V   G  NNEML  +M+ +G
Sbjct:  164 VRLLAHLEHRQHVVMVFESLEMNLRETLRKFGKHVGLNISAVRTYAKQLLFALRHIEKLGIVHADIKPDNILVSEGFGQLKLCDFGSAFREDAPDNAPTPYLVSRFYRAPEVILGFEYGRGVDLWSVGCCLYELFTGRVTLPGSSNNEMLRLIMELKG 321
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A7S2WEY6_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WEY6_9STRA)

HSP 1 Score: 174 bits (440), Expect = 2.530e-51
Identity = 84/158 (53.16%), Postives = 103/158 (65.19%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARG 492
            + LL  F  K H  LVFE+  M++R+ L KFG+ VG+ + +VR Y R LF AL  +  L VVHAD+KPDN+LV        L DFGSAF+  DPDN PT  L+SRFYR PE+ LG + D R+DLWS    LFELFTG V+F GRDNN ML   M  +G
Sbjct:    5 IHLLTNFEHKSHTALVFESYAMNLRETLKKFGKNVGINIKSVRIYARQLFIALRHIASLRVVHADIKPDNILVSEDFRTLKLCDFGSAFKETDPDNDPTPYLVSRFYRAPEITLGLEYDRRIDLWSVGVCLFELFTGSVMFPGRDNNHMLQVFMDMKG 162
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A5A8CB33_CAFRO (Uncharacterized protein n=5 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CB33_CAFRO)

HSP 1 Score: 179 bits (453), Expect = 1.430e-49
Identity = 85/165 (51.52%), Postives = 112/165 (67.88%), Query Frame = 1 
Query:    4 SDPC-TVRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARGP 495
             D C  VRL G F  + HLCLVFE +++++R+++ +FG+ VGL   AVR  GR L  AL +L   G+VHAD+KP N++V++    C L D GSAF  D PDN P   L+SRFYR PEV LGF+    +DLWS  CVLFE++TG VLF G DN++MLW +M  +GP
Sbjct:  207 EDRCHVVRLRGYFEWRRHLCLVFEAMQLNLRELIDRFGKHVGLAATAVRALGRQLLIALRLLERQGIVHADIKPQNIVVNSDRTSCKLCDLGSAFAVDGPDNVPAPLLVSRFYRAPEVILGFQHTAALDLWSLGCVLFEMYTGSVLFDGADNSDMLWRIMALQGP 371
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A087SFR2_AUXPR (Non-specific serine/threonine protein kinase n=2 Tax=Auxenochlorella protothecoides TaxID=3075 RepID=A0A087SFR2_AUXPR)

HSP 1 Score: 174 bits (440), Expect = 2.070e-49
Identity = 86/168 (51.19%), Postives = 113/168 (67.26%), Query Frame = 1 
Query:    4 SDPC----TVRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARGP 495
            +DP      VRLLG F  + HLCLVFE+L+M++R++  ++GRG+GL LGAVR Y   +   L+ L   GV+HAD+KPDN+LV+A   +  L DFGSA  + D  N  T  L+SRFYR PEV LG   D  +D+W   CV+FELFTG++LF G+ NNEML  MM  +GP
Sbjct:  180 ADPAGKRHCVRLLGSFDYRSHLCLVFESLDMNLRELTKRYGRGIGLSLGAVRLYAHQMLVGLYHLQACGVLHADIKPDNILVNAKRTQVKLCDFGSAMFSGD--NEITPYLVSRFYRAPEVILGLPYDHPMDMWGVGCVIFELFTGRILFPGKTNNEMLKLMMDVKGP 345
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A6H5KYV7_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KYV7_9PHAE)

HSP 1 Score: 167 bits (424), Expect = 2.550e-49
Identity = 78/159 (49.06%), Postives = 108/159 (67.92%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARGP 495
            +RLL     K H+ +VFE+++M++R+ L KFGR  G+ + AV++YG+ L  AL  +    +VHAD+KPDN+LVD   G   L+DFGSAFR  DPDN PT  L+SR+YR PE+ +G + D  +DLWS A  LFEL+ G  +  G +NNEML  MM+ +GP
Sbjct:   34 IRLLCSLEHKQHVVMVFESMQMNLRETLKKFGRKTGINIHAVQKYGKQLLAALKHMASRNLVHADIKPDNILVDQGFGSLKLADFGSAFRITDPDNDPTPYLVSRWYRAPEIVMGLEYDKAIDLWSVAVTLFELYKGSPVLPGVNNNEMLKRMMEFKGP 192
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A197K135_9FUNG (Non-specific serine/threonine protein kinase n=1 Tax=Linnemannia elongata AG-77 TaxID=1314771 RepID=A0A197K135_9FUNG)

HSP 1 Score: 171 bits (433), Expect = 3.690e-49
Identity = 85/168 (50.60%), Postives = 113/168 (67.26%), Query Frame = 1 
Query:    4 SDPCT----VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARGP 495
            +DP      +RLL  F  +GHLCLVFE+L M++R+VL KFG+ +GL + AVR Y + LF AL +L    V+HAD+KPDN+LV+ S     L D GSA  +D  +N  T  L+SRFYR PE+ LG   D  +D+WS  C L+ELFTG++LF+GR NN+ML  MM  +GP
Sbjct:  100 NDPLNKKHIIRLLRHFEHRGHLCLVFESLSMNLREVLKKFGKDIGLNINAVRIYAQQLFLALSLLKKSNVLHADIKPDNILVNDSKNILKLCDLGSA--SDTSENEITPYLVSRFYRAPEIILGMPYDPALDMWSIGCTLYELFTGKILFSGRSNNQMLKHMMDLKGP 265
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A4D9DES0_9STRA (Protein kinase domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DES0_9STRA)

HSP 1 Score: 177 bits (450), Expect = 4.050e-49
Identity = 85/158 (53.80%), Postives = 108/158 (68.35%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARG 492
            VRLL     + H+ +VFE+L+M++R+ L KFG+ VGL + AVR Y + L  AL  +  LG+VHAD+KPDN+LV    G+  L DFGSAFR D PDN PT  L+SRFYR PEV LGF+    VDLWS  C L+ELFTG+V   G  NNEML  +M+ +G
Sbjct:  454 VRLLAHLEHRQHVVMVFESLQMNLRETLRKFGKHVGLNISAVRTYAKQLLFALRHIEKLGIVHADIKPDNILVSEGFGQLKLCDFGSAFREDAPDNEPTPYLVSRFYRAPEVILGFEYGRGVDLWSVGCCLYELFTGRVTLPGSSNNEMLRLIMELKG 611
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: I0ZAX9_COCSC (Non-specific serine/threonine protein kinase n=1 Tax=Coccomyxa subellipsoidea (strain C-169) TaxID=574566 RepID=I0ZAX9_COCSC)

HSP 1 Score: 171 bits (433), Expect = 4.360e-49
Identity = 84/158 (53.16%), Postives = 108/158 (68.35%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARG 492
            VRLL  F  + HLCLVFEN+++++R++  K+GRG+GL + AVR Y   +  AL+ L   GV+HAD+KPDN+LV AS     L DFGSA    D  N  T  L+SRFYR PEV LG K D  +D+WS  CV++ELFTG++LF GR NNEML  MM  +G
Sbjct:  117 VRLLRTFEYRQHLCLVFENMDINLRELTKKYGRGIGLNIAAVRAYAAQMLTALYHLRNCGVLHADIKPDNILVSASRTMVKLCDFGSAMFTGD--NETTPYLVSRFYRSPEVILGLKYDHPMDMWSVGCVIYELFTGKILFPGRSNNEMLKLMMDVKG 272
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BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: D8LRH1_ECTSI (ATP binding / protein kinase/ protein serine/threonine kinase/ protein-tyrosine kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRH1_ECTSI)

HSP 1 Score: 167 bits (424), Expect = 1.440e-48
Identity = 78/159 (49.06%), Postives = 108/159 (67.92%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARGP 495
            +RLL     K H+ +VFE+++M++R+ L KFGR  G+ + AV++YG+ L  AL  +    +VHAD+KPDN+LVD   G   L+DFGSAFR  DPDN PT  L+SR+YR PE+ +G + D  +DLWS A  LFEL+ G  +  G +NNEML  MM+ +GP
Sbjct:   34 IRLLCSLEHKQHVVMVFESMQMNLRETLKKFGRKTGINIHAVQKYGKQLLAALKHMASRNLVHADIKPDNILVDQGFGSLKLADFGSAFRITDPDNDPTPYLVSRWYRAPEIVMGLEYDKAIDLWSVAVTLFELYKGSPVLPGVNNNEMLKRMMEFKGP 192
[Close]
BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Match: A0A7S2RWC8_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RWC8_9STRA)

HSP 1 Score: 174 bits (440), Expect = 2.460e-48
Identity = 84/158 (53.16%), Postives = 103/158 (65.19%), Query Frame = 1 
Query:   19 VRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVREYGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDPDNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGRDNNEMLWAMMQARG 492
            + LL  F  K H  LVFE+  M++R+ L KFG+ VG+ + +VR Y R LF AL  +  L VVHAD+KPDN+LV        L DFGSAF+  DPDN PT  L+SRFYR PE+ LG + D R+DLWS    LFELFTG V+F GRDNN ML   M  +G
Sbjct:  330 IHLLTNFEHKSHTALVFESYAMNLRETLKKFGKNVGINIKSVRIYARQLFIALRHIASLRVVHADIKPDNILVSEDFRTLKLCDFGSAFKETDPDNDPTPYLVSRFYRAPEITLGLEYDRRIDLWSVGVCLFELFTGSVMFPGRDNNHMLQVFMDMKG 487
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125581.5177.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
K8YZQ6_NANGC1.330e-5154.43Protein kinase domain-containing protein n=2 Tax=N... [more]
A0A7S2WEY6_9STRA2.530e-5153.16Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A5A8CB33_CAFRO1.430e-4951.52Uncharacterized protein n=5 Tax=Cafeteria roenberg... [more]
A0A087SFR2_AUXPR2.070e-4951.19Non-specific serine/threonine protein kinase n=2 T... [more]
A0A6H5KYV7_9PHAE2.550e-4949.06Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A197K135_9FUNG3.690e-4950.60Non-specific serine/threonine protein kinase n=1 T... [more]
A0A4D9DES0_9STRA4.050e-4953.80Protein kinase domain-containing protein n=1 Tax=N... [more]
I0ZAX9_COCSC4.360e-4953.16Non-specific serine/threonine protein kinase n=1 T... [more]
D8LRH1_ECTSI1.440e-4849.06ATP binding / protein kinase/ protein serine/threo... [more]
A0A7S2RWC8_9STRA2.460e-4853.16Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125581contigM-pyrifera_M_contig125581:1..495 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score179.5
Seed ortholog evalue1.3e-42
Seed eggNOG ortholog3075.A0A087SFR2
KEGG koko:K08827
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0006464,GO:0006468,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009987,GO:0016310,GO:0019538,GO:0036211,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044464,GO:0071704,GO:1901564
EggNOG free text desc.Protein tyrosine kinase
EggNOG OGs34GR3@3041,37P0V@33090,KOG0667@1,KOG0670@2759
EC2.7.11.1
COG Functional cat.A
Best tax levelChlorophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01001,ko03041
Exons1
Model size495
Cds size495
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815771.8349473-CDS-M-pyrifera_M_contig125581:0..4951622815771.8349473-CDS-M-pyrifera_M_contig125581:0..495Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125581 1..495 -
1692277561.974602-CDS-M-pyrifera_M_contig125581:0..4951692277561.974602-CDS-M-pyrifera_M_contig125581:0..495Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125581 1..495 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125581.5177.1prot_M-pyrifera_M_contig125581.5177.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125581 1..495 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125581.5177.1

>prot_M-pyrifera_M_contig125581.5177.1 ID=prot_M-pyrifera_M_contig125581.5177.1|Name=mRNA_M-pyrifera_M_contig125581.5177.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
MSDPCTVRLLGQFVDKGHLCLVFENLEMSVRDVLLKFGRGVGLRLGAVRE
YGRALFKALHVLHVLGVVHADVKPDNMLVDASLGRCVLSDFGSAFRADDP
DNSPTQTLISRFYRPPEVCLGFKPDTRVDLWSAACVLFELFTGQVLFAGR
DNNEMLWAMMQARGP
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mRNA from alignment at M-pyrifera_M_contig125581:1..495-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125581.5177.1 ID=mRNA_M-pyrifera_M_contig125581.5177.1|Name=mRNA_M-pyrifera_M_contig125581.5177.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=495bp|location=Sequence derived from alignment at M-pyrifera_M_contig125581:1..495- (Macrocystis pyrifera P11B4 male)
ATGTCTGATCCGTGCACTGTTCGTTTGCTGGGCCAGTTTGTGGACAAGGG
GCACTTGTGTTTGGTGTTTGAGAACTTGGAAATGTCTGTGCGTGACGTGC
TGCTGAAGTTTGGTCGTGGTGTTGGGTTGCGCTTGGGAGCGGTACGGGAG
TATGGGCGAGCCTTGTTCAAGGCGTTGCATGTGCTGCATGTGCTGGGAGT
GGTGCATGCAGATGTGAAGCCGGACAACATGCTGGTGGACGCGAGCTTGG
GACGCTGTGTGCTGTCCGATTTTGGGAGTGCCTTTCGCGCAGATGACCCT
GACAATTCTCCGACGCAAACCCTCATTTCTCGGTTCTACAGACCTCCAGA
AGTGTGTCTTGGGTTCAAGCCAGACACGAGAGTGGACTTGTGGAGTGCGG
CGTGTGTGTTGTTTGAGCTATTCACGGGACAGGTGCTCTTTGCCGGGAGG
GACAACAACGAGATGCTTTGGGCGATGATGCAAGCCAGAGGGCCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125581:1..495-

>mRNA_M-pyrifera_M_contig125581.5177.1 ID=mRNA_M-pyrifera_M_contig125581.5177.1|Name=mRNA_M-pyrifera_M_contig125581.5177.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig125581:1..495- (Macrocystis pyrifera P11B4 male)
ATGTCTGATCCGTGCACTGTTCGTTTGCTGGGCCAGTTTGTGGACAAGGG
GCACTTGTGTTTGGTGTTTGAGAACTTGGAAATGTCTGTGCGTGACGTGC
TGCTGAAGTTTGGTCGTGGTGTTGGGTTGCGCTTGGGAGCGGTACGGGAG
TATGGGCGAGCCTTGTTCAAGGCGTTGCATGTGCTGCATGTGCTGGGAGT
GGTGCATGCAGATGTGAAGCCGGACAACATGCTGGTGGACGCGAGCTTGG
GACGCTGTGTGCTGTCCGATTTTGGGAGTGCCTTTCGCGCAGATGACCCT
GACAATTCTCCGACGCAAACCCTCATTTCTCGGTTCTACAGACCTCCAGA
AGTGTGTCTTGGGTTCAAGCCAGACACGAGAGTGGACTTGTGGAGTGCGG
CGTGTGTGTTGTTTGAGCTATTCACGGGACAGGTGCTCTTTGCCGGGAGG
GACAACAACGAGATGCTTTGGGCGATGATGCAAGCCAGAGGGCCTATGTC
TGATCCGTGCACTGTTCGTTTGCTGGGCCAGTTTGTGGACAAGGGGCACT
TGTGTTTGGTGTTTGAGAACTTGGAAATGTCTGTGCGTGACGTGCTGCTG
AAGTTTGGTCGTGGTGTTGGGTTGCGCTTGGGAGCGGTACGGGAGTATGG
GCGAGCCTTGTTCAAGGCGTTGCATGTGCTGCATGTGCTGGGAGTGGTGC
ATGCAGATGTGAAGCCGGACAACATGCTGGTGGACGCGAGCTTGGGACGC
TGTGTGCTGTCCGATTTTGGGAGTGCCTTTCGCGCAGATGACCCTGACAA
TTCTCCGACGCAAACCCTCATTTCTCGGTTCTACAGACCTCCAGAAGTGT
GTCTTGGGTTCAAGCCAGACACGAGAGTGGACTTGTGGAGTGCGGCGTGT
GTGTTGTTTGAGCTATTCACGGGACAGGTGCTCTTTGCCGGGAGGGACAA
CAACGAGATGCTTTGGGCGATGATGCAAGCCAGAGGGCCT
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