mRNA_M-pyrifera_M_contig125247.5109.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125247.5109.1
Unique NamemRNA_M-pyrifera_M_contig125247.5109.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: F0ZRK0_DICPU (Carm_PH domain-containing protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZRK0_DICPU)

HSP 1 Score: 123 bits (309), Expect = 1.360e-29
Identity = 70/162 (43.21%), Postives = 98/162 (60.49%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYL---QGSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEP-RLLKTLDLIQATLARNQNP 474
            +K L  + NF     K R  A+E++ITL+  + PI  L++    G   + LK DIL  +  + +N +LL +DISG  MGNKGAIALGKA+QTN TL  L++DEN TG+ G      G++RN +LK M  P+ DI A  +  P +L +TL  I   + RNQ+P
Sbjct:  501 IKKLVLNGNFKTSKTKSRAAAIESVITLLESECPIESLHITAGSGKSLSPLKNDILPLVYSLATNSSLLEIDISGHQMGNKGAIALGKALQTNKTLHTLIWDENQTGVVGFSGFQVGLERNLTLKNMPTPLTDIIAAHRENPVKLSQTLKEIDNCINRNQSP 662
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: D3BTX1_POLPP (Leucine-rich repeat-containing protein n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3BTX1_POLPP)

HSP 1 Score: 122 bits (306), Expect = 3.460e-29
Identity = 68/160 (42.50%), Postives = 99/160 (61.88%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYL-QGSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEP-RLLKTLDLIQATLARNQNP 474
            +K L  + NF     K R  A+E++I L+   +P+  L++  G+  + LK D+L F+  + +N TL+ LDISG  MGNKGAIALGKA+QTN +L+ LV+DEN TG+ G   +  G++RN +LK M  P+LDI       P +L + L  I   + RNQ+P
Sbjct:  499 VKKLVLNGNFKVSKTKSRAAAIESVINLLEASTPLEALHITNGASKSQLKNDLLPFIYALATNDTLVELDISGHQMGNKGAIALGKALQTNKSLQTLVWDENQTGVAGFAGLTVGLERNSTLKNMPKPLLDIMKAYNENPVKLSQLLKEIDHFINRNQSP 658
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: A0A151ZH21_9MYCE (Leucine-rich repeat-containing protein (LRR) n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151ZH21_9MYCE)

HSP 1 Score: 118 bits (296), Expect = 7.640e-28
Identity = 68/160 (42.50%), Postives = 97/160 (60.62%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYLQ-GSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEP-RLLKTLDLIQATLARNQNP 474
            +K L  + NF     K R  ++E++ITL+  + PI  L+L  G   + LK DIL  +  + +N +LL LDISG  MGNKGAIALGKA+QTN TL  L +D+N  G+PG V +  G++RN +LK M  P+ DI    +  P +L   +  I+  + RNQ+P
Sbjct:  498 IKKLVLNGNFKTSKTKSRAASIESVITLLESECPIEILHLTVGQSKSPLKNDILPLVYSLATNDSLLELDISGHQMGNKGAIALGKALQTNKTLHTLFWDDNLIGIPGYVGLQVGLERNLTLKNMPTPLNDICQAYRENPIKLQSIIKEIERCINRNQSP 657
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: P90630_ACACA (Myosin-I binding protein Acan125 n=2 Tax=Acanthamoeba castellanii TaxID=5755 RepID=P90630_ACACA)

HSP 1 Score: 118 bits (295), Expect = 1.040e-27
Identity = 71/171 (41.52%), Postives = 99/171 (57.89%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYLQGSKT-NSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAI-----PKSEPRLLKTLDLIQATLARNQNPKSTLGQG 495
            L+ L    N++    K R+ AV+ LI LIS + P+  L L      N +K DIL F+  + +N TL  LDISG AMG+K  IALGKA Q N TL  L++D NGT   G + + NG++RN  L  M +P+ DIS +     P+S  ++ + +  I   +ARNQ+PKS   QG
Sbjct:  514 LRELHLGDNWTRNKTKARSQAVDNLIELISSECPLHKLDLSCKVADNQIKTDILPFIYSLATNDTLKELDISGNAMGDKVGIALGKAFQINRTLSSLIWDRNGTRYAGFLGLKNGLERNNVLVNMPVPVFDISDLLKNEKPESALQVQQVVLHISEYIARNQSPKSKFAQG 684
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: UPI000644BDDE (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644BDDE)

HSP 1 Score: 117 bits (293), Expect = 1.980e-27
Identity = 67/152 (44.08%), Postives = 92/152 (60.53%), Query Frame = 1 
Query:   25 NFSPKPMKQRTTAVEALITLISEQSPISHLYL-QGSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDI-SAIPKSEPRLLKTLDLIQATLARNQNP 474
            NF     K R   VE++I L+   +P+  L++  GS    LK D+L  +  + +N TL+ LDI+G  MGNKGAIALGKA+QTN  L+ LV+DEN TG+ G +    G++RN +LK M  P+LDI  A  +S  RL   L  I   + RNQ+P
Sbjct:  503 NFKVSKTKSRAATVESVIGLLEASTPLEALHMTNGSGKYHLKNDLLPLIYALATNDTLVELDITGHLMGNKGAIALGKALQTNKALQTLVWDENATGVVGFIGFTTGLERNLTLKNMPKPLLDIMKAYSESPVRLSNLLKDIDHYINRNQSP 654
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: A0A8J4V199_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4V199_9MYCE)

HSP 1 Score: 116 bits (290), Expect = 4.880e-27
Identity = 66/160 (41.25%), Postives = 98/160 (61.25%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYLQ-GSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEP-RLLKTLDLIQATLARNQNP 474
            +K L  + NF     K R+ ++E++ITL+  + PI  L++  G+  + LK DIL  +  + +N +LL LDI+G  MG KGAIALGKA+QTN TL  L++DEN T + G    + G++RN +LK M+ P+ DI A  +  P +L   L  I   + RNQ+P
Sbjct:  494 VKRLLLNGNFKTSKTKSRSASIESVITLLESECPIETLHITAGNSKSPLKNDILPLIYSLATNSSLLELDITGHQMGVKGAIALGKALQTNKTLHTLIWDENLTTLAGFSGFLVGLERNLTLKNMQTPLTDIIAAHRENPTKLQSVLKDIDNCINRNQSP 653
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: UPI000644EBD7 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644EBD7)

HSP 1 Score: 115 bits (287), Expect = 1.240e-26
Identity = 66/163 (40.49%), Postives = 95/163 (58.28%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYL-QGSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEP-RLLKTLDLIQATLARNQNPKST 483
            +K L  + NF     K R  A+E++I+L+   +P+  L++  G   + LK DIL F+  + +N +L  LDI+G  MGNKGAIALGKA+QTN  L  LV+DEN T + G   +  G++RN +LK M  P++DI       P RL   +  I   + RNQ+P  T
Sbjct:  495 VKKLSLNGNFKVSKTKSRAAAIESVISLVEAATPLEALHMTNGLSKSQLKNDILPFIYALATNDSLTELDITGHQMGNKGAIALGKALQTNKALVSLVWDENLTSVAGFAGLAVGLERNLTLKNMNKPLIDIMKAYNENPQRLTNLIRDIDNYINRNQSPLRT 657
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: F4PR98_CAVFA (Leucine-rich repeat-containing protein n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4PR98_CAVFA)

HSP 1 Score: 109 bits (273), Expect = 9.390e-25
Identity = 62/160 (38.75%), Postives = 94/160 (58.75%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYL-QGSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEP-RLLKTLDLIQATLARNQNP 474
            +K L  + NF     K R +A+E+++ L+   + +  L++  G+    LK +IL  +  + +N +LL LDISG  MGNKGAIALGKA+QTN  L  LV+D+N TG+ G   +  G++RN +LK M  P++DI    +  P +L   +  I   + RNQ P
Sbjct:  496 VKRLVLNGNFKVSKTKSRPSAIESVVNLLEAGTSLESLHMTNGTSKTQLKTEILPIIYSLATNDSLLELDISGHQMGNKGAIALGKALQTNKALNTLVWDDNMTGLAGFAGLQVGLERNLTLKNMPKPLVDIMQAHRENPAKLTAIIKDIDYCINRNQAP 655
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: CARML_DICDI (Protein CARMIL n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=CARML_DICDI)

HSP 1 Score: 108 bits (269), Expect = 3.230e-24
Identity = 62/159 (38.99%), Postives = 94/159 (59.12%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYLQ-GSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEPRLLKTLDLIQATLARNQNP 474
            +K L  + NF     K R +A+E++I+L+  + P+  L++  G+  + LK DIL  +  + +N +LL LDISG  MG KGAI LGKA+QTN TL  L++D+N T   G      G++RN +LK M  P+ DI    + EP+  +    I + + RNQ+P
Sbjct:  501 IKKLILNGNFKQSKTKSRPSAIESVISLLESECPLETLHMTVGNSKSPLKADILSLIYSLATNSSLLELDISGHQMGPKGAIGLGKALQTNKTLHTLIWDDNLTTAIGFAGFQVGLERNLTLKNMPTPLNDIIQCHR-EPKFQQIWKEIDSCINRNQSP 658
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BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Match: A0A2P6NI38_9EUKA (Leucine-rich repeat-containing protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NI38_9EUKA)

HSP 1 Score: 103 bits (257), Expect = 1.360e-22
Identity = 59/162 (36.42%), Postives = 96/162 (59.26%), Query Frame = 1 
Query:    1 LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYLQGSKTNSLKLDILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDENGTGMPGLVAVVNGMQRNRSLKQMRLPILDI-SAIPKSEPR-LLKTLDLIQATLARNQNPKS 480
            +K L    NF  +  ++    +E LI LI  ++ +  L + G   + L+ D+L  +  + +N TL  +DISG  MGNKGA ALGKA+QTN TL+ L +D N T   G  + + G++RN +LK M +PI DI +A+  +E + + +++  ++  + RNQ+P S
Sbjct:  492 IKHLNIDANFKTRAKEKSKGFIEPLIALIGSETALESLSIAGGLKSELRTDVLPLIYALATNNTLKSIDISGHLMGNKGAAALGKALQTNETLDTLRWDGNATTAVGFSSFLIGLKRNFTLKSMVVPIYDIGNALKGAEVQDITRSVSEMEKLMIRNQSPVS 653
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125247.5109.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
F0ZRK0_DICPU1.360e-2943.21Carm_PH domain-containing protein n=1 Tax=Dictyost... [more]
D3BTX1_POLPP3.460e-2942.50Leucine-rich repeat-containing protein n=1 Tax=Pol... [more]
A0A151ZH21_9MYCE7.640e-2842.50Leucine-rich repeat-containing protein (LRR) n=1 T... [more]
P90630_ACACA1.040e-2741.52Myosin-I binding protein Acan125 n=2 Tax=Acanthamo... [more]
UPI000644BDDE1.980e-2744.08hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A8J4V199_9MYCE4.880e-2741.25Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
UPI000644EBD71.240e-2640.49hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
F4PR98_CAVFA9.390e-2538.75Leucine-rich repeat-containing protein n=1 Tax=Cav... [more]
CARML_DICDI3.230e-2438.99Protein CARMIL n=1 Tax=Dictyostelium discoideum Ta... [more]
A0A2P6NI38_9EUKA1.360e-2236.42Leucine-rich repeat-containing protein n=1 Tax=Pla... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125247contigM-pyrifera_M_contig125247:1..495 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score131.7
Seed ortholog evalue3.1e-28
Seed eggNOG ortholog529818.AMSG_00532T0
KEGG koko:K20493
Hectar predicted targeting categorymitochondrion
GOsGO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005856,GO:0005885,GO:0006996,GO:0007010,GO:0007015,GO:0008064,GO:0008092,GO:0008150,GO:0008290,GO:0009987,GO:0010638,GO:0015629,GO:0016043,GO:0017022,GO:0017024,GO:0030029,GO:0030036,GO:0030139,GO:0030832,GO:0030833,GO:0030838,GO:0031334,GO:0031410,GO:0031982,GO:0032271,GO:0032273,GO:0032535,GO:0032956,GO:0032970,GO:0032991,GO:0033043,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043232,GO:0043254,GO:0044087,GO:0044089,GO:0044422,GO:0044424,GO:0044430,GO:0044444,GO:0044446,GO:0044464,GO:0045010,GO:0045335,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051128,GO:0051130,GO:0051493,GO:0051495,GO:0065007,GO:0065008,GO:0071840,GO:0090066,GO:0097435,GO:0097708,GO:0110053,GO:1902903,GO:1902905
EggNOG free text desc.barbed-end actin filament uncapping
EggNOG OGsKOG4242@1,KOG4242@2759
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Exons1
Model size495
Cds size495
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815768.5198076-CDS-M-pyrifera_M_contig125247:0..4951622815768.5198076-CDS-M-pyrifera_M_contig125247:0..495Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125247 1..495 -
1692277560.4982188-CDS-M-pyrifera_M_contig125247:0..4951692277560.4982188-CDS-M-pyrifera_M_contig125247:0..495Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125247 1..495 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125247.5109.1prot_M-pyrifera_M_contig125247.5109.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125247 1..495 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125247.5109.1

>prot_M-pyrifera_M_contig125247.5109.1 ID=prot_M-pyrifera_M_contig125247.5109.1|Name=mRNA_M-pyrifera_M_contig125247.5109.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
LKTLRFSRNFSPKPMKQRTTAVEALITLISEQSPISHLYLQGSKTNSLKL
DILDFLDGVGSNKTLLFLDISGQAMGNKGAIALGKAIQTNHTLEHLVFDE
NGTGMPGLVAVVNGMQRNRSLKQMRLPILDISAIPKSEPRLLKTLDLIQA
TLARNQNPKSTLGQG
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mRNA from alignment at M-pyrifera_M_contig125247:1..495-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125247.5109.1 ID=mRNA_M-pyrifera_M_contig125247.5109.1|Name=mRNA_M-pyrifera_M_contig125247.5109.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=495bp|location=Sequence derived from alignment at M-pyrifera_M_contig125247:1..495- (Macrocystis pyrifera P11B4 male)
TTGAAGACCCTCCGCTTCAGCAGGAACTTCTCCCCCAAGCCCATGAAGCA
GAGGACCACTGCGGTTGAGGCGCTCATCACTCTCATCAGCGAGCAGTCCC
CCATCTCTCACCTCTACTTGCAGGGCAGCAAGACCAACTCACTCAAGCTG
GACATCCTGGACTTCCTTGATGGCGTGGGTTCGAACAAGACGCTCCTGTT
CCTGGACATCTCCGGCCAGGCGATGGGCAACAAGGGCGCCATTGCGCTGG
GCAAGGCCATCCAGACCAACCACACCCTCGAGCACTTGGTCTTCGACGAG
AACGGCACTGGCATGCCGGGGCTGGTGGCTGTTGTCAATGGCATGCAGCG
CAACCGCAGCCTCAAGCAGATGCGCCTCCCCATTCTGGACATCAGCGCCA
TCCCCAAGTCTGAACCTCGGCTCCTGAAGACTCTGGACCTCATCCAGGCA
ACCCTTGCCCGCAACCAGAACCCCAAGTCCACTCTTGGCCAAGGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125247:1..495-

>mRNA_M-pyrifera_M_contig125247.5109.1 ID=mRNA_M-pyrifera_M_contig125247.5109.1|Name=mRNA_M-pyrifera_M_contig125247.5109.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig125247:1..495- (Macrocystis pyrifera P11B4 male)
TTGAAGACCCTCCGCTTCAGCAGGAACTTCTCCCCCAAGCCCATGAAGCA
GAGGACCACTGCGGTTGAGGCGCTCATCACTCTCATCAGCGAGCAGTCCC
CCATCTCTCACCTCTACTTGCAGGGCAGCAAGACCAACTCACTCAAGCTG
GACATCCTGGACTTCCTTGATGGCGTGGGTTCGAACAAGACGCTCCTGTT
CCTGGACATCTCCGGCCAGGCGATGGGCAACAAGGGCGCCATTGCGCTGG
GCAAGGCCATCCAGACCAACCACACCCTCGAGCACTTGGTCTTCGACGAG
AACGGCACTGGCATGCCGGGGCTGGTGGCTGTTGTCAATGGCATGCAGCG
CAACCGCAGCCTCAAGCAGATGCGCCTCCCCATTCTGGACATCAGCGCCA
TCCCCAAGTCTGAACCTCGGCTCCTGAAGACTCTGGACCTCATCCAGGCA
ACCCTTGCCCGCAACCAGAACCCCAAGTCCACTCTTGGCCAAGGTTTGAA
GACCCTCCGCTTCAGCAGGAACTTCTCCCCCAAGCCCATGAAGCAGAGGA
CCACTGCGGTTGAGGCGCTCATCACTCTCATCAGCGAGCAGTCCCCCATC
TCTCACCTCTACTTGCAGGGCAGCAAGACCAACTCACTCAAGCTGGACAT
CCTGGACTTCCTTGATGGCGTGGGTTCGAACAAGACGCTCCTGTTCCTGG
ACATCTCCGGCCAGGCGATGGGCAACAAGGGCGCCATTGCGCTGGGCAAG
GCCATCCAGACCAACCACACCCTCGAGCACTTGGTCTTCGACGAGAACGG
CACTGGCATGCCGGGGCTGGTGGCTGTTGTCAATGGCATGCAGCGCAACC
GCAGCCTCAAGCAGATGCGCCTCCCCATTCTGGACATCAGCGCCATCCCC
AAGTCTGAACCTCGGCTCCTGAAGACTCTGGACCTCATCCAGGCAACCCT
TGCCCGCAACCAGAACCCCAAGTCCACTCTTGGCCAAGGT
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