mRNA_M-pyrifera_M_contig12324.4771.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig12324.4771.1
Unique NamemRNA_M-pyrifera_M_contig12324.4771.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: D7FUS1_ECTSI (Nitrate/nitrite transporter n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FUS1_ECTSI)

HSP 1 Score: 181 bits (458), Expect = 3.360e-53
Identity = 85/95 (89.47%), Postives = 93/95 (97.89%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            LADDCPKGNYKELEAHGAMTRKSSAVSF+KGY NVNSW+MF QYAACFGVELT+NNVAA+YFSDEFGLSTSKAG+VASLFGLMN+FARS+GG+WS
Sbjct:  217 LADDCPKGNYKELEAHGAMTRKSSAVSFKKGYSNVNSWIMFAQYAACFGVELTVNNVAANYFSDEFGLSTSKAGMVASLFGLMNLFARSVGGIWS 311
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: D7FUR8_ECTSI (Nitrate high affinity transporter n=4 Tax=Ectocarpus TaxID=2879 RepID=D7FUR8_ECTSI)

HSP 1 Score: 177 bits (449), Expect = 7.260e-52
Identity = 85/95 (89.47%), Postives = 92/95 (96.84%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            LADD PKGNYKELEAHGAMTRKSSAVSF+KGY+NVNSWLMF QYAACFGVELT+NNVAA+YFSDEF LSTSKAG+VASLFGLMN+FARSLGG+WS
Sbjct:  217 LADDSPKGNYKELEAHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYFSDEFDLSTSKAGLVASLFGLMNLFARSLGGIWS 311
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: E9KZG4_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured Chaetoceros TaxID=941234 RepID=E9KZG4_9STRA)

HSP 1 Score: 129 bits (324), Expect = 2.900e-36
Identity = 58/95 (61.05%), Postives = 73/95 (76.84%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            ++DDCPKGNYKE++  G MT  S++ SFR G +N N+WL+F QYA CFGVELTMNN +A+YF +EFG ST  A  +AS+FG MN+FAR LGG  S
Sbjct:   54 ISDDCPKGNYKEMKEKGIMTEVSASASFRDGAMNFNTWLLFIQYACCFGVELTMNNASATYFREEFGQSTESAAAIASIFGWMNLFARGLGGFTS 148
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: A4URC6_9STRA (NRT2 (Fragment) n=2 Tax=Chaetoceros muellerii TaxID=265525 RepID=A4URC6_9STRA)

HSP 1 Score: 127 bits (319), Expect = 1.070e-34
Identity = 58/95 (61.05%), Postives = 71/95 (74.74%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            ++DDCPKGNYKE++ +G M   S+A SFR G +N N+WL+F QY  CFGVELTMNN AASYF + F LST  A  +AS+FG MN+FAR LGG  S
Sbjct:   80 ISDDCPKGNYKEMKQNGIMNEISAAASFRDGALNFNTWLLFIQYGCCFGVELTMNNAAASYFKETFDLSTESAAAIASIFGWMNLFARGLGGFTS 174
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: A0A448YWL2_9STRA (Nitrate/nitrite transporter n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448YWL2_9STRA)

HSP 1 Score: 131 bits (329), Expect = 2.210e-34
Identity = 60/95 (63.16%), Postives = 72/95 (75.79%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            ++DD PKGNY EL+ HG M   S+A SFR+G VN N+W++F QYA CFGVELTMNN AA YF D+FGLST  A  +AS+FG MN+FAR LGG  S
Sbjct:  186 VSDDAPKGNYSELKKHGQMADVSAAASFRQGAVNFNTWILFIQYACCFGVELTMNNAAALYFKDKFGLSTESAAAIASIFGFMNLFARGLGGFMS 280
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: E9KZI2_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured diatom TaxID=137771 RepID=E9KZI2_9STRA)

HSP 1 Score: 124 bits (311), Expect = 2.960e-34
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1 
Query:    4 ADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGG 276
            +DDCPKG YK+L  HG M   S+A SFR+G +N N+WL+F QYA CFGVELTMNN AA YF  +F L+T  A  +AS+FG MNIFAR LGG
Sbjct:   58 SDDCPKGQYKDLMKHGTMADVSAAASFRQGALNFNTWLLFIQYACCFGVELTMNNAAALYFKQKFELTTESAAAIASIFGFMNIFARGLGG 148
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BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: A0A7S2VF05_9STRA (Hypothetical protein (Fragment) n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S2VF05_9STRA)

HSP 1 Score: 122 bits (305), Expect = 7.310e-34
Identity = 56/90 (62.22%), Postives = 67/90 (74.44%), Query Frame = 1 
Query:   16 PKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            PKGNY +L+ HG M   S+A SFR G +N N+W++F QYA CFGVELTMNN AA YF DEFG ST  A  +AS+FG MN+FAR LGG +S
Sbjct:    2 PKGNYSDLKKHGNMPEVSAAASFRSGAINFNTWVLFIQYACCFGVELTMNNAAALYFKDEFGQSTESAAAIASIFGWMNLFARGLGGYFS 91
[Close]
BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: E9KZH5_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured diatom TaxID=137771 RepID=E9KZH5_9STRA)

HSP 1 Score: 122 bits (307), Expect = 1.070e-33
Identity = 54/95 (56.84%), Postives = 70/95 (73.68%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            ++DDCPKGNYK+++  G M   S++ SFR G +N+N+WL+F QY  CFGVELTMNN AASYF + F  +T  A  +AS+FG MN+FAR LGG  S
Sbjct:   53 ISDDCPKGNYKDMKEKGIMQEVSASASFRDGAMNINTWLLFIQYGCCFGVELTMNNAAASYFEETFNQTTESAAAIASIFGWMNLFARGLGGFTS 147
[Close]
BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: A4URC9_9STRA (NRT2 (Fragment) n=2 Tax=Chaetoceros muellerii TaxID=265525 RepID=A4URC9_9STRA)

HSP 1 Score: 124 bits (311), Expect = 1.720e-33
Identity = 56/95 (58.95%), Postives = 72/95 (75.79%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            ++DDCP+GN++EL+ HG M   S++ SF +  +N N+WL F Q+A CFGVELTMNN AA+YF +EF LST KA  + S+FGLMNIFAR LGG  S
Sbjct:   80 ISDDCPRGNFRELKRHGVMNHVSASASFHEVAMNFNTWLFFLQHACCFGVELTMNNSAATYFHEEFNLSTEKAAAITSIFGLMNIFARGLGGFIS 174
[Close]
BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Match: E9KZG6_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured Rhizosolenia TaxID=941242 RepID=E9KZG6_9STRA)

HSP 1 Score: 122 bits (305), Expect = 2.210e-33
Identity = 56/95 (58.95%), Postives = 70/95 (73.68%), Query Frame = 1 
Query:    1 LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGVELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS 285
            ++DD PKGNY EL+ HG+M   S+A SFR+G  N N+WL+F QYA CFGVELTMNN AA YF + FG +T  A  +AS+FG MN+FAR +GG  S
Sbjct:   54 ISDDSPKGNYAELKKHGSMADVSAAASFRQGAFNFNTWLLFIQYACCFGVELTMNNAAALYFKEVFGQTTESAAAIASIFGWMNLFARGVGGFCS 148
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig12324.4771.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FUS1_ECTSI3.360e-5389.47Nitrate/nitrite transporter n=2 Tax=Ectocarpus Tax... [more]
D7FUR8_ECTSI7.260e-5289.47Nitrate high affinity transporter n=4 Tax=Ectocarp... [more]
E9KZG4_9STRA2.900e-3661.05High affinity nitrate transporter (Fragment) n=1 T... [more]
A4URC6_9STRA1.070e-3461.05NRT2 (Fragment) n=2 Tax=Chaetoceros muellerii TaxI... [more]
A0A448YWL2_9STRA2.210e-3463.16Nitrate/nitrite transporter n=1 Tax=Pseudo-nitzsch... [more]
E9KZI2_9STRA2.960e-3462.64High affinity nitrate transporter (Fragment) n=1 T... [more]
A0A7S2VF05_9STRA7.310e-3462.22Hypothetical protein (Fragment) n=1 Tax=Amphiprora... [more]
E9KZH5_9STRA1.070e-3356.84High affinity nitrate transporter (Fragment) n=1 T... [more]
A4URC9_9STRA1.720e-3358.95NRT2 (Fragment) n=2 Tax=Chaetoceros muellerii TaxI... [more]
E9KZG6_9STRA2.210e-3358.95High affinity nitrate transporter (Fragment) n=1 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig12324contigM-pyrifera_M_contig12324:8495..9360 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score183.7
Seed ortholog evalue3.9e-44
Seed eggNOG ortholog2880.D7FUS2
Preferred nameNRT2.2
KEGG koko:K02575
KEGG TC2.A.1.8
KEGG Pathwayko00910,map00910
KEGG ModuleM00615
Hectar predicted targeting categoryother localisation
GOsGO:0000325,GO:0001101,GO:0003674,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005774,GO:0005886,GO:0006810,GO:0006811,GO:0006820,GO:0007275,GO:0008150,GO:0008509,GO:0009507,GO:0009536,GO:0009628,GO:0009705,GO:0009791,GO:0009987,GO:0010035,GO:0010167,GO:0015075,GO:0015103,GO:0015112,GO:0015267,GO:0015291,GO:0015318,GO:0015513,GO:0015698,GO:0015706,GO:0015707,GO:0016020,GO:0022622,GO:0022803,GO:0022804,GO:0022832,GO:0022836,GO:0022857,GO:0031090,GO:0032501,GO:0032502,GO:0032991,GO:0034220,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0048364,GO:0048527,GO:0048528,GO:0048731,GO:0048856,GO:0050896,GO:0051179,GO:0051234,GO:0051716,GO:0055085,GO:0070887,GO:0071229,GO:0071241,GO:0071249,GO:0071705,GO:0071944,GO:0080167,GO:0090696,GO:0098588,GO:0098656,GO:0098805,GO:0099402,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1902170,GO:1990351
EggNOG free text desc.cellular response to nitrate
EggNOG OGs2QPIC@2759,COG2223@1
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Exons2
Model size285
Cds size285
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815751.5831313-CDS-M-pyrifera_M_contig12324:8494..86891622815751.5831313-CDS-M-pyrifera_M_contig12324:8494..8689Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig12324 8495..8689 -
1692277552.406725-CDS-M-pyrifera_M_contig12324:8494..86891692277552.406725-CDS-M-pyrifera_M_contig12324:8494..8689Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig12324 8495..8689 -
1622815751.5935917-CDS-M-pyrifera_M_contig12324:9270..93601622815751.5935917-CDS-M-pyrifera_M_contig12324:9270..9360Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig12324 9271..9360 -
1692277552.416273-CDS-M-pyrifera_M_contig12324:9270..93601692277552.416273-CDS-M-pyrifera_M_contig12324:9270..9360Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig12324 9271..9360 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig12324.4771.1prot_M-pyrifera_M_contig12324.4771.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig12324 8495..9360 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig12324.4771.1

>prot_M-pyrifera_M_contig12324.4771.1 ID=prot_M-pyrifera_M_contig12324.4771.1|Name=mRNA_M-pyrifera_M_contig12324.4771.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=95bp
LADDCPKGNYKELEAHGAMTRKSSAVSFRKGYVNVNSWLMFCQYAACFGV
ELTMNNVAASYFSDEFGLSTSKAGIVASLFGLMNIFARSLGGMWS
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mRNA from alignment at M-pyrifera_M_contig12324:8495..9360-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig12324.4771.1 ID=mRNA_M-pyrifera_M_contig12324.4771.1|Name=mRNA_M-pyrifera_M_contig12324.4771.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=866bp|location=Sequence derived from alignment at M-pyrifera_M_contig12324:8495..9360- (Macrocystis pyrifera P11B4 male)
CTCGCCGACGACTGCCCCAAGGGTAACTACAAAGAGCTGGAGGCGCACGG
CGCCATGACCCGAAAGTCTTCCGCTGTGTCCTTCAGGAAGGTACGGCGTT
GTGGCAACAATGATATGACAATGGTTGGTTCCGTCAAGCGAACGTGCTAT
AGTGGCAGGTCAATTCGATATGTTTACGCTGCCGATGTGTACGAATGCGC
CAGTCAACCGTAGACACCTCTCGATATATACCACGCGTAGGGGAGGTATC
TTCGACGTCGATGTAAAATCATGTAAAGTAGTGGGCGAGTACACCAGCCA
ACCGCAAGCATCTCTCGAACTTTGTGTGTGCGAGGAACATGTGTAAACGA
GCGACGCAATAAAAACGAGATATCAAACAACGCACGAAAGATACGAAAAA
CCCACGGAAAAATCAAAGTACACCTGGTGAGCACCAAGTATAATTTTGCT
GTGTTCTCACGATGACTCATTTTACTGTGAGCACCAAGTATAATTTCGCT
GTGTTTTCACGATTACTCGTTTCACCCAACCCTCGCTTTGCGCTTGGGCG
CCGTGCTTCGGTCCCAGAATGACCGCACTCGCACGAAGGTTCACCTTGCC
CACCCACCCACCTACCCCCCGCTCTGTCCCCCGCGCGCTTTGGTGTATTT
TGTTTTTTCCCCGTCGAGAAGGGATACGTGAATGTGAACTCGTGGTTGAT
GTTCTGTCAATACGCGGCGTGCTTCGGCGTGGAGCTGACTATGAATAACG
TGGCGGCCTCTTACTTCTCGGACGAGTTCGGCCTGTCCACGTCCAAGGCG
GGCATCGTGGCTTCGCTGTTCGGTCTCATGAACATCTTCGCACGCTCGTT
GGGCGGCATGTGGTCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig12324:8495..9360-

>mRNA_M-pyrifera_M_contig12324.4771.1 ID=mRNA_M-pyrifera_M_contig12324.4771.1|Name=mRNA_M-pyrifera_M_contig12324.4771.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig12324:8495..9360- (Macrocystis pyrifera P11B4 male)
CTCGCCGACGACTGCCCCAAGGGTAACTACAAAGAGCTGGAGGCGCACGG
CGCCATGACCCGAAAGTCTTCCGCTGTGTCCTTCAGGAAGCTCGCCGACG
ACTGCCCCAAGGGTAACTACAAAGAGCTGGAGGCGCACGGCGCCATGACC
CGAAAGTCTTCCGCTGTGTCCTTCAGGAAGGGATACGTGAATGTGAACTC
GTGGTTGATGTTCTGTCAATACGCGGCGTGCTTCGGCGTGGAGCTGACTA
TGAATAACGTGGCGGCCTCTTACTTCTCGGACGAGTTCGGCCTGTCCACG
TCCAAGGCGGGCATCGTGGCTTCGCTGTTCGGTCTCATGAACATCTTCGC
ACGCTCGTTGGGCGGCATGTGGTCCGGATACGTGAATGTGAACTCGTGGT
TGATGTTCTGTCAATACGCGGCGTGCTTCGGCGTGGAGCTGACTATGAAT
AACGTGGCGGCCTCTTACTTCTCGGACGAGTTCGGCCTGTCCACGTCCAA
GGCGGGCATCGTGGCTTCGCTGTTCGGTCTCATGAACATCTTCGCACGCT
CGTTGGGCGGCATGTGGTCC
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