mRNA_M-pyrifera_M_contig122952.4715.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig122952.4715.1
Unique NamemRNA_M-pyrifera_M_contig122952.4715.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: UPI001929C350 (DNA topoisomerase 3-beta-like n=2 Tax=Dioscorea cayennensis subsp. rotundata TaxID=55577 RepID=UPI001929C350)

HSP 1 Score: 143 bits (360), Expect = 1.010e-40
Identity = 69/119 (57.98%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:   13 VLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            VLMVAEKPSIA ++A+AL+GG   +H+    VHE+ G F GF   + VTSV GH+FS DFPP YQN E  DP+ L+ APVLK     K  + RH+ Q A G  +LVLWLDCDREGENIC
Sbjct:    5 VLMVAEKPSIALSIASALAGGRMSTHKSSTDVHEFDGTFMGFDVHYKVTSVIGHVFSLDFPPSYQNWEVTDPMDLFQAPVLKSESNPKAHIHRHLSQEARGCGYLVLWLDCDREGENIC 123
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A2U1Q1B1_ARTAN (DNA topoisomerase n=1 Tax=Artemisia annua TaxID=35608 RepID=A0A2U1Q1B1_ARTAN)

HSP 1 Score: 137 bits (345), Expect = 1.160e-38
Identity = 68/123 (55.28%), Postives = 84/123 (68.29%), Query Frame = 1 
Query:    1 MVVTVLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            M   VLMVAEKPSIA ++A ALSGG   S R    VHE+ G F G+R ++ VTSV GH+FS DFPP+YQ+    DP+TL+ APV+K     K  + +H+ + A G   LVLWLDCDREGENIC
Sbjct:    1 MATKVLMVAEKPSIALSIATALSGGRMSSRRGSTEVHEFDGTFLGYRVQYKVTSVIGHVFSVDFPPKYQDWAATDPLTLFEAPVVKSESNPKAHIRQHLNREARGCGDLVLWLDCDREGENIC 123
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A3P6AXE7_BRAOL (DNA topoisomerase n=1 Tax=Brassica oleracea TaxID=3712 RepID=A0A3P6AXE7_BRAOL)

HSP 1 Score: 134 bits (338), Expect = 4.380e-38
Identity = 64/119 (53.78%), Postives = 81/119 (68.07%), Query Frame = 1 
Query:   13 VLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            VLMVAEKPSIA ++A  LS G+  + R    VHE+ G FRGF+A + VTSV GH+FS DFP +YQN   +DP  L+ AP+ K+    K  + RH+   A G  ++VLWLDCDREGENIC
Sbjct:    7 VLMVAEKPSIALSIATVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDFPEKYQNWSTIDPQDLFDAPIQKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENIC 125
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A699IS67_TANCI (DNA topoisomerase (Fragment) n=1 Tax=Tanacetum cinerariifolium TaxID=118510 RepID=A0A699IS67_TANCI)

HSP 1 Score: 137 bits (345), Expect = 7.130e-38
Identity = 68/123 (55.28%), Postives = 84/123 (68.29%), Query Frame = 1 
Query:    1 MVVTVLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            M   VLMVAEKPSIA ++A ALSGG   S R    VHE+ G F G+R ++ VTSV GH+FS DFPP+YQ+    DP+TL+ APV+K     K  + +H+ + A G   LVLWLDCDREGENIC
Sbjct:   29 MATKVLMVAEKPSIALSIATALSGGRMSSRRGSTEVHEFDGTFLGYRVQYKVTSVIGHVFSVDFPPKYQDWAATDPLTLFEAPVVKSESNPKAHIRQHLNREARGCGDLVLWLDCDREGENIC 151
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A1J4MI51_9CRYT (DNA topoisomerase n=1 Tax=Cryptosporidium ubiquitum TaxID=857276 RepID=A0A1J4MI51_9CRYT)

HSP 1 Score: 144 bits (364), Expect = 9.510e-38
Identity = 64/119 (53.78%), Postives = 86/119 (72.27%), Query Frame = 1 
Query:    7 VTVLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAEKGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            + VLMVAEKPSI+ T++  LS G+  + R   PVHE++G+FRG   +F VTSVAGH+F T+FP  Y N EK DPV+L+ AP++K      M+ H+++ + G  +LVLWLDCDREGENIC
Sbjct:    4 IKVLMVAEKPSISDTISKILSNGKLVTRRAKTPVHEFNGNFRGMNVQFKVTSVAGHVFETNFPQSYSNWEKTDPVSLFDAPIIKNESSSKMISHLEKESKGCSYLVLWLDCDREGENIC 122
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A8B7C6T9_PHODC (DNA topoisomerase n=5 Tax=Arecaceae TaxID=4710 RepID=A0A8B7C6T9_PHODC)

HSP 1 Score: 144 bits (364), Expect = 9.760e-38
Identity = 72/119 (60.50%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:   13 VLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            VLMVAEKPSIA +VA+ALSGG   + R    VHE+ G FRG  A F VTSV GH+FS DFPP YQN E  DP+ L+ AP+LK     K  + RH+ Q A G  +LVLWLDCDREGENIC
Sbjct:    5 VLMVAEKPSIALSVASALSGGRMSTRRGSTDVHEFDGMFRGLHAHFKVTSVIGHVFSVDFPPTYQNWEVTDPMDLFQAPILKSESNPKAHIRRHLSQEAKGCGYLVLWLDCDREGENIC 123
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A6I9RI61_ELAGV (DNA topoisomerase n=4 Tax=Elaeis guineensis var. tenera TaxID=51953 RepID=A0A6I9RI61_ELAGV)

HSP 1 Score: 144 bits (364), Expect = 9.770e-38
Identity = 72/119 (60.50%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:   13 VLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            VLMVAEKPSIA ++A+ALSGG   + R    VHE+ G FRG  A F VTSV GHIFS DFPP YQN E  DP+ L+ AP+LK     K  + RH+ Q A G  +LVLWLDCDREGENIC
Sbjct:    5 VLMVAEKPSIALSIASALSGGRMSTRRGSTDVHEFDGMFRGLHAHFKVTSVIGHIFSVDFPPTYQNWEVTDPMDLFQAPILKSESNPKAHIRRHLSQEAKGCGYLVLWLDCDREGENIC 123
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BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: A0A835V3K0_VANPL (DNA topoisomerase n=2 Tax=Vanilla planifolia TaxID=51239 RepID=A0A835V3K0_VANPL)

HSP 1 Score: 144 bits (363), Expect = 1.330e-37
Identity = 71/123 (57.72%), Postives = 87/123 (70.73%), Query Frame = 1 
Query:    1 MVVTVLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            MV  VLMVAEKPSIA ++A+ALS G   + R    VHE+ G F+G +A F VTSV GH++S DFPP+YQN +  DP+ L+ APVLK     KG + RH+ Q A G  +LVLWLDCDREGENIC
Sbjct:    1 MVHKVLMVAEKPSIAVSIASALSNGRMSTRRGSTDVHEFDGTFQGNQAHFKVTSVTGHVYSVDFPPKYQNWDLTDPIDLFQAPVLKLESNPKGHICRHLSQEARGCTYLVLWLDCDREGENIC 123
[Close]
BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: UPI0018ADECA9 (DNA topoisomerase 3-beta n=1 Tax=Dioscorea cayennensis subsp. rotundata TaxID=55577 RepID=UPI0018ADECA9)

HSP 1 Score: 143 bits (360), Expect = 3.390e-37
Identity = 70/119 (58.82%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:   13 VLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAE--KGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            VLMVAEKPSIA ++A+ALSGG   + +    VHE+ G FRGF   + VTSV GH+FS DFPP YQN E  DP+ L+ APVLK     K  + RH+ Q A G  +LVLWLDCDREGENIC
Sbjct:    5 VLMVAEKPSIALSIASALSGGRMSTRKSSTDVHEFDGTFRGFDVHYKVTSVIGHVFSLDFPPTYQNWEVTDPMDLFQAPVLKSESNPKAHIRRHLSQEARGCGYLVLWLDCDREGENIC 123
[Close]
BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Match: L1JH36_GUITC (DNA topoisomerase n=4 Tax=Guillardia theta TaxID=55529 RepID=L1JH36_GUITC)

HSP 1 Score: 142 bits (359), Expect = 4.390e-37
Identity = 72/119 (60.50%), Postives = 85/119 (71.43%), Query Frame = 1 
Query:    7 VTVLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARFVVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAEKGSMVRHIQQSAAGAHFLVLWLDCDREGENIC 363
            VTV+MVAEKPSI   +A ALSGG  E+     PVH +  +F G  A F VTSV GH+FSTDFP  YQ+ +KVDPVTL+ APV K+AEK  +V  +Q    GA +LVLWLDCDREGENIC
Sbjct:    2 VTVVMVAEKPSICNAIAIALSGGNHETKGGTPPVHTFRSNFMGSSALFKVTSVVGHVFSTDFPSAYQSWDKVDPVTLFDAPVEKKAEKKGVVHLLQNEGKGADYLVLWLDCDREGENIC 120
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig122952.4715.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001929C3501.010e-4057.98DNA topoisomerase 3-beta-like n=2 Tax=Dioscorea ca... [more]
A0A2U1Q1B1_ARTAN1.160e-3855.28DNA topoisomerase n=1 Tax=Artemisia annua TaxID=35... [more]
A0A3P6AXE7_BRAOL4.380e-3853.78DNA topoisomerase n=1 Tax=Brassica oleracea TaxID=... [more]
A0A699IS67_TANCI7.130e-3855.28DNA topoisomerase (Fragment) n=1 Tax=Tanacetum cin... [more]
A0A1J4MI51_9CRYT9.510e-3853.78DNA topoisomerase n=1 Tax=Cryptosporidium ubiquitu... [more]
A0A8B7C6T9_PHODC9.760e-3860.50DNA topoisomerase n=5 Tax=Arecaceae TaxID=4710 Rep... [more]
A0A6I9RI61_ELAGV9.770e-3860.50DNA topoisomerase n=4 Tax=Elaeis guineensis var. t... [more]
A0A835V3K0_VANPL1.330e-3757.72DNA topoisomerase n=2 Tax=Vanilla planifolia TaxID... [more]
UPI0018ADECA93.390e-3758.82DNA topoisomerase 3-beta n=1 Tax=Dioscorea cayenne... [more]
L1JH36_GUITC4.390e-3760.50DNA topoisomerase n=4 Tax=Guillardia theta TaxID=5... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig122952contigM-pyrifera_M_contig122952:163..525 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score144.4
Seed ortholog evalue3.4e-32
Seed eggNOG ortholog42345.XP_008792952.1
KEGG koko:K03165
KEGG Pathwayko03440,ko03460,map03440,map03460
KEGG ModuleM00414
Hectar predicted targeting categoryother localisation
GOsGO:0000793,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0003824,GO:0003916,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0006139,GO:0006259,GO:0006265,GO:0006725,GO:0006807,GO:0006996,GO:0007059,GO:0008150,GO:0008152,GO:0009987,GO:0016043,GO:0016853,GO:0034641,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0044237,GO:0044238,GO:0044260,GO:0044424,GO:0044464,GO:0046483,GO:0051276,GO:0071103,GO:0071704,GO:0071840,GO:0090304,GO:0097159,GO:0140097,GO:1901360,GO:1901363
EggNOG free text desc.Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
EggNOG OGs37M7P@33090,3GFMV@35493,3KPPD@4447,COG0550@1,KOG1957@2759
EC5.99.1.2
COG Functional cat.L
Best tax levelLiliopsida
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400
Exons1
Model size363
Cds size363
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815748.5483565-CDS-M-pyrifera_M_contig122952:162..5251622815748.5483565-CDS-M-pyrifera_M_contig122952:162..525Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig122952 163..525 +
1692277551.0399206-CDS-M-pyrifera_M_contig122952:162..5251692277551.0399206-CDS-M-pyrifera_M_contig122952:162..525Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig122952 163..525 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig122952.4715.1prot_M-pyrifera_M_contig122952.4715.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig122952 163..525 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig122952.4715.1

>prot_M-pyrifera_M_contig122952.4715.1 ID=prot_M-pyrifera_M_contig122952.4715.1|Name=mRNA_M-pyrifera_M_contig122952.4715.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=121bp
MVVTVLMVAEKPSIAATVANALSGGEFESHRHGMPVHEYSGDFRGFRARF
VVTSVAGHIFSTDFPPEYQNREKVDPVTLYAAPVLKRAEKGSMVRHIQQS
AAGAHFLVLWLDCDREGENIC
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mRNA from alignment at M-pyrifera_M_contig122952:163..525+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig122952.4715.1 ID=mRNA_M-pyrifera_M_contig122952.4715.1|Name=mRNA_M-pyrifera_M_contig122952.4715.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=363bp|location=Sequence derived from alignment at M-pyrifera_M_contig122952:163..525+ (Macrocystis pyrifera P11B4 male)
ATGGTTGTCACCGTGTTAATGGTGGCTGAGAAGCCATCGATCGCTGCCAC
AGTGGCCAATGCCCTGTCTGGCGGGGAGTTCGAGTCCCATCGCCATGGCA
TGCCCGTGCACGAATACAGCGGCGACTTTCGCGGCTTCCGCGCCCGCTTC
GTCGTCACATCTGTAGCTGGCCACATCTTCAGCACCGACTTTCCGCCCGA
GTACCAGAACAGGGAGAAGGTTGACCCTGTGACGCTGTATGCGGCGCCAG
TGCTGAAACGAGCGGAGAAGGGCAGCATGGTGCGCCACATCCAGCAGTCT
GCCGCCGGCGCGCACTTCCTTGTGCTGTGGCTGGACTGTGACCGCGAAGG
AGAGAACATCTGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig122952:163..525+

>mRNA_M-pyrifera_M_contig122952.4715.1 ID=mRNA_M-pyrifera_M_contig122952.4715.1|Name=mRNA_M-pyrifera_M_contig122952.4715.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=726bp|location=Sequence derived from alignment at M-pyrifera_M_contig122952:163..525+ (Macrocystis pyrifera P11B4 male)
ATGGTTGTCACCGTGTTAATGGTGGCTGAGAAGCCATCGATCGCTGCCAC
AGTGGCCAATGCCCTGTCTGGCGGGGAGTTCGAGTCCCATCGCCATGGCA
TGCCCGTGCACGAATACAGCGGCGACTTTCGCGGCTTCCGCGCCCGCTTC
GTCGTCACATCTGTAGCTGGCCACATCTTCAGCACCGACTTTCCGCCCGA
GTACCAGAACAGGGAGAAGGTTGACCCTGTGACGCTGTATGCGGCGCCAG
TGCTGAAACGAGCGGAGAAGGGCAGCATGGTGCGCCACATCCAGCAGTCT
GCCGCCGGCGCGCACTTCCTTGTGCTGTGGCTGGACTGTGACCGCGAAGG
AGAGAACATCTGCATGGTTGTCACCGTGTTAATGGTGGCTGAGAAGCCAT
CGATCGCTGCCACAGTGGCCAATGCCCTGTCTGGCGGGGAGTTCGAGTCC
CATCGCCATGGCATGCCCGTGCACGAATACAGCGGCGACTTTCGCGGCTT
CCGCGCCCGCTTCGTCGTCACATCTGTAGCTGGCCACATCTTCAGCACCG
ACTTTCCGCCCGAGTACCAGAACAGGGAGAAGGTTGACCCTGTGACGCTG
TATGCGGCGCCAGTGCTGAAACGAGCGGAGAAGGGCAGCATGGTGCGCCA
CATCCAGCAGTCTGCCGCCGGCGCGCACTTCCTTGTGCTGTGGCTGGACT
GTGACCGCGAAGGAGAGAACATCTGC
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