mRNA_M-pyrifera_M_contig121614.4442.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig121614.4442.1
Unique NamemRNA_M-pyrifera_M_contig121614.4442.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: UPI000345688A (enoyl-CoA hydratase n=1 Tax=Ilumatobacter nonamiensis TaxID=467093 RepID=UPI000345688A)

HSP 1 Score: 289 bits (740), Expect = 1.630e-96
Identity = 145/177 (81.92%), Postives = 155/177 (87.57%), Query Frame = 1 
Query:    1 LDIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            LD+A+RIATITLNRPDARNALSS+VL K+  AM RAESDD  DV+ILTGTDPAFTAGLDLKELG+SG  L  GTGADG VNA G RGPFP L KPLIGA+NGVAVTGG E AL CDFLVASE AKFGDTHARVGVMPGWGLTVLLPQAIG+RRAREMSFTGNF+ A EAL+ GLVNH
Sbjct:   21 LDVADRIATITLNRPDARNALSSEVLTKLSTAMRRAESDDAADVLILTGTDPAFTAGLDLKELGSSGDNLGAGTGADGSVNADGIRGPFPKLTKPLIGAINGVAVTGGFELALNCDFLVASENAKFGDTHARVGVMPGWGLTVLLPQAIGIRRAREMSFTGNFMDAHEALQFGLVNH 197
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A524B309_9ACTN (Enoyl-CoA hydratase n=1 Tax=Acidimicrobiales bacterium mtb01 TaxID=1967499 RepID=A0A524B309_9ACTN)

HSP 1 Score: 274 bits (700), Expect = 2.090e-90
Identity = 132/176 (75.00%), Postives = 152/176 (86.36%), Query Frame = 1 
Query:    4 DIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            D++ERIAT+TLNRPDARNALS+DVLR++P  +  A   DDVDVI+LTGTDPAF AGLDLKELG++G  L  G+GADG  N+ G RGPFP ++KPLIGAVNGVA+TGG E AL CDFL+ASE AKFGDTH+RVGVMPGWGLTVLLPQAIG+RRAREMSFTGNF+ A+EAL  GLVNH
Sbjct:   24 DVSERIATVTLNRPDARNALSTDVLRELPRCLREANDRDDVDVIVLTGTDPAFCAGLDLKELGSTGGNLGSGSGADGRPNSDGVRGPFPMVDKPLIGAVNGVAITGGFELALNCDFLIASEHAKFGDTHSRVGVMPGWGLTVLLPQAIGLRRAREMSFTGNFMSADEALAFGLVNH 199
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A6J6ZE99_9ZZZZ (Unannotated protein n=2 Tax=root TaxID=1 RepID=A0A6J6ZE99_9ZZZZ)

HSP 1 Score: 273 bits (699), Expect = 2.130e-90
Identity = 137/177 (77.40%), Postives = 153/177 (86.44%), Query Frame = 1 
Query:    1 LDIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            L+I+ERIATITLNRP ARNALS++VL+ +P  M RA++ DDVDVIILTG+DPAF+AGLDLKELG++G  L  GTGADG  NA G RGPFP L+KPLIGA+NGVAVTGG E AL CDFL+ASE AKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNF+ A EA   GLVNH
Sbjct:   13 LEISERIATITLNRPSARNALSTEVLKLLPQLMKRADASDDVDVIILTGSDPAFSAGLDLKELGSTGDNLGAGTGADGRPNADGIRGPFPALKKPLIGAINGVAVTGGFELALNCDFLIASEHAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFMLAPEAYDRGLVNH 189
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A3C2B509_9ACTN (Enoyl-CoA hydratase n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A3C2B509_9ACTN)

HSP 1 Score: 273 bits (697), Expect = 3.520e-90
Identity = 135/176 (76.70%), Postives = 153/176 (86.93%), Query Frame = 1 
Query:    4 DIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            +++ERIAT+TLNRP+ARNALSS+VLR +P  +  AE+DD+VDVIILTGTDPAF AGLDLKELG+SG  L  G+GADG  NA G RGPFP L KPLIGAVNGVA+TGG E AL CDFLVASE AKFGDTH+RVGVMPGWGLTVLLPQAIGVRRAREMS TGNF+ A+EAL+ G+VNH
Sbjct:    8 EVSERIATVTLNRPEARNALSSEVLRLLPDRLRAAEADDEVDVIILTGTDPAFCAGLDLKELGSSGGNLGGGSGADGRPNAEGRRGPFPTLTKPLIGAVNGVAITGGFELALNCDFLVASEHAKFGDTHSRVGVMPGWGLTVLLPQAIGVRRAREMSLTGNFMGADEALQFGMVNH 183
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A6L6CVC9_9ACTN (Enoyl-CoA hydratase (Fragment) n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A6L6CVC9_9ACTN)

HSP 1 Score: 269 bits (688), Expect = 6.370e-90
Identity = 132/176 (75.00%), Postives = 153/176 (86.93%), Query Frame = 1 
Query:    4 DIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            +++ERIATIT NRP ARNALSS+VL+ +P  ML+A++DD+VDV+ILTG+DPAF AGLDLKELG +   L  GTGADGGVN+ G RGPFP + KPLIGAVNGVA+TGG E AL CDFL+ASE AKFGDTH+RVG+MPGWGLTVLLPQAIGVRRAREMSFTGNF+ A+EAL  GLVNH
Sbjct:    8 EVSERIATITFNRPAARNALSSEVLKLLPQLMLKADADDNVDVLILTGSDPAFCAGLDLKELGDTAGNLG-GTGADGGVNSTGVRGPFPKISKPLIGAVNGVAITGGFELALNCDFLIASEHAKFGDTHSRVGIMPGWGLTVLLPQAIGVRRAREMSFTGNFMLADEALAFGLVNH 182
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A7K1B3S5_9ACTN (Enoyl-CoA hydratase n=2 Tax=root TaxID=1 RepID=A0A7K1B3S5_9ACTN)

HSP 1 Score: 272 bits (695), Expect = 7.570e-90
Identity = 135/177 (76.27%), Postives = 152/177 (85.88%), Query Frame = 1 
Query:    1 LDIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            L+++ RIATITLNRP ARNALSS+VLR +PA M  A++ DDVDV+ILTG DPAF AGLDLKELG++   L  GTGADG  NA G RGPFPP++KP+IGA+NGVA+TGG E AL CDFLVASE AKFGDTH RVGVMPGWGLTVLLPQAIGVRRAREMSFTGNF+ A+EALR GLVNH
Sbjct:   10 LEVSARIATITLNRPAARNALSSEVLRLLPALMREADARDDVDVVILTGADPAFCAGLDLKELGSTAGNLG-GTGADGSPNAAGVRGPFPPMDKPVIGAINGVAITGGFELALNCDFLVASEHAKFGDTHTRVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFMSADEALRWGLVNH 185
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A6I3HK17_9ACTN (Enoyl-CoA hydratase n=4 Tax=root TaxID=1 RepID=A0A6I3HK17_9ACTN)

HSP 1 Score: 270 bits (691), Expect = 2.870e-89
Identity = 136/176 (77.27%), Postives = 151/176 (85.80%), Query Frame = 1 
Query:    4 DIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            +++ERIATIT+NRP ARNALSS+VL+ +PA M  A+ DD VDVIILTGTDPAF AGLDLKELG +G  L  GTGADG  N+ GARGPFP + KPLIGAVNGVA+TGG E AL CDFL+ASE AKFGDTH+RVGVMPGWGLTVLLPQAIGVRRAREMSFTGNF+ AEEAL  GLVNH
Sbjct:    9 EVSERIATITMNRPAARNALSSEVLKLLPAFMKTADKDDSVDVIILTGTDPAFCAGLDLKELGDTGGNL-RGTGADGSKNSSGARGPFPRVSKPLIGAVNGVAITGGFELALNCDFLIASENAKFGDTHSRVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFMLAEEALAFGLVNH 183
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A7K1C9Q0_9ACTN (Enoyl-CoA hydratase n=2 Tax=root TaxID=1 RepID=A0A7K1C9Q0_9ACTN)

HSP 1 Score: 270 bits (691), Expect = 3.500e-89
Identity = 136/177 (76.84%), Postives = 151/177 (85.31%), Query Frame = 1 
Query:    1 LDIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            LD+ ERIATITLNRP ARNALSS+VL+ +P  M +A++ DDVDVIILTG+DPAF AGLDL+ELG+SG  L  G+GADG  N+ G RGPFP + KPLIGAVNGVAVTGG E AL CDFLVASE AKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMS TGNF+ A+EAL  GLVNH
Sbjct:   13 LDVDERIATITLNRPAARNALSSEVLKLIPRLMKQADTSDDVDVIILTGSDPAFCAGLDLRELGSSGKNLGSGSGADGRPNSDGVRGPFPGVSKPLIGAVNGVAVTGGFELALNCDFLVASEHAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSMTGNFMLADEALARGLVNH 189
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BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A4P5S2S1_9ACTN (Putative enoyl-CoA hydratase n=9 Tax=root TaxID=1 RepID=A0A4P5S2S1_9ACTN)

HSP 1 Score: 269 bits (688), Expect = 1.000e-88
Identity = 136/177 (76.84%), Postives = 152/177 (85.88%), Query Frame = 1 
Query:    1 LDIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            L+I ERIATITLNRP ARNALS DVL+ +P  M +A + +DVDVIILTG+DPAF AGLDLKELG++G+ L  G+GADG  N+ G RGPFP L+KPLIGAVNGVA+TGG E AL CDFLVASE AKFGDTHARVGVMPGWGLTVLLPQAIG+RRAREMSFTGNF+ AEEAL  GLVNH
Sbjct:   13 LEINERIATITLNRPAARNALSFDVLKLLPQLMKQANASEDVDVIILTGSDPAFCAGLDLKELGSTGANLGSGSGADGRPNSDGVRGPFPLLDKPLIGAVNGVAITGGFELALNCDFLVASENAKFGDTHARVGVMPGWGLTVLLPQAIGLRRAREMSFTGNFMLAEEALTRGLVNH 189
[Close]
BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Match: A0A6L6CFN8_9ACTN (Enoyl-CoA hydratase n=3 Tax=root TaxID=1 RepID=A0A6L6CFN8_9ACTN)

HSP 1 Score: 268 bits (685), Expect = 2.270e-88
Identity = 132/176 (75.00%), Postives = 152/176 (86.36%), Query Frame = 1 
Query:    4 DIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGTDPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAVNGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIGVRRAREMSFTGNFLPAEEALRCGLVNH 531
            +++ERIATIT NRP ARNALSS+VL  +P  ML+A++DD+VDV+ILTG+DPAF AGLDLKELG +   L  GTGADGGVN+ G RGPFP + KPLIGAVNGVA+TGG E AL CDFL+ASE AKFGDTH+RVG+MPGWGLTVLLPQAIGVRRAREMSFTGNF+ A+EAL  GLVNH
Sbjct:    8 EVSERIATITFNRPAARNALSSEVLTLLPQLMLKADADDNVDVLILTGSDPAFCAGLDLKELGDTAGNLG-GTGADGGVNSTGVRGPFPKISKPLIGAVNGVAITGGFELALNCDFLIASEHAKFGDTHSRVGIMPGWGLTVLLPQAIGVRRAREMSFTGNFMLADEALAFGLVNH 182
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig121614.4442.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI000345688A1.630e-9681.92enoyl-CoA hydratase n=1 Tax=Ilumatobacter nonamien... [more]
A0A524B309_9ACTN2.090e-9075.00Enoyl-CoA hydratase n=1 Tax=Acidimicrobiales bacte... [more]
A0A6J6ZE99_9ZZZZ2.130e-9077.40Unannotated protein n=2 Tax=root TaxID=1 RepID=A0A... [more]
A0A3C2B509_9ACTN3.520e-9076.70Enoyl-CoA hydratase n=1 Tax=Acidimicrobiaceae bact... [more]
A0A6L6CVC9_9ACTN6.370e-9075.00Enoyl-CoA hydratase (Fragment) n=1 Tax=Actinomycet... [more]
A0A7K1B3S5_9ACTN7.570e-9076.27Enoyl-CoA hydratase n=2 Tax=root TaxID=1 RepID=A0A... [more]
A0A6I3HK17_9ACTN2.870e-8977.27Enoyl-CoA hydratase n=4 Tax=root TaxID=1 RepID=A0A... [more]
A0A7K1C9Q0_9ACTN3.500e-8976.84Enoyl-CoA hydratase n=2 Tax=root TaxID=1 RepID=A0A... [more]
A0A4P5S2S1_9ACTN1.000e-8876.84Putative enoyl-CoA hydratase n=9 Tax=root TaxID=1 ... [more]
A0A6L6CFN8_9ACTN2.270e-8875.00Enoyl-CoA hydratase n=3 Tax=root TaxID=1 RepID=A0A... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig121614contigM-pyrifera_M_contig121614:2..532 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score268.9
Seed ortholog evalue1.7e-69
Seed eggNOG ortholog1313172.YM304_11240
KEGG rclassRC00831,RC00834,RC01086,RC01095,RC01098,RC01103,RC01217,RC02115
KEGG koko:K01692
KEGG ReactionR03026,R03045,R04137,R04170,R04204,R04224,R04738,R04740,R04744,R04746,R04749,R05595,R06411,R06412,R06942,R08093
KEGG Pathwayko00071,ko00280,ko00281,ko00310,ko00360,ko00362,ko00380,ko00410,ko00627,ko00640,ko00650,ko00903,ko00930,ko01100,ko01110,ko01120,ko01130,ko01212,map00071,map00280,map00281,map00310,map00360,map00362,map00380,map00410,map00627,map00640,map00650,map00903,map00930,map01100,map01110,map01120,map01130,map01212
KEGG ModuleM00032,M00087
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Enoyl-CoA hydratase/isomerase
EggNOG OGs2GJ0N@201174,4CNYM@84992,COG1024@1,COG1024@2
EC4.2.1.17
COG Functional cat.I
Best tax levelAcidimicrobiia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size531
Cds size531
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815735.374565-CDS-M-pyrifera_M_contig121614:1..5321622815735.374565-CDS-M-pyrifera_M_contig121614:1..532Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig121614 2..532 +
1692277544.8266854-CDS-M-pyrifera_M_contig121614:1..5321692277544.8266854-CDS-M-pyrifera_M_contig121614:1..532Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig121614 2..532 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig121614.4442.1prot_M-pyrifera_M_contig121614.4442.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig121614 2..532 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig121614.4442.1

>prot_M-pyrifera_M_contig121614.4442.1 ID=prot_M-pyrifera_M_contig121614.4442.1|Name=mRNA_M-pyrifera_M_contig121614.4442.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=177bp
LDIAERIATITLNRPDARNALSSDVLRKVPAAMLRAESDDDVDVIILTGT
DPAFTAGLDLKELGTSGSMLTEGTGADGGVNAFGARGPFPPLEKPLIGAV
NGVAVTGGLEFALACDFLVASERAKFGDTHARVGVMPGWGLTVLLPQAIG
VRRAREMSFTGNFLPAEEALRCGLVNH
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mRNA from alignment at M-pyrifera_M_contig121614:2..532+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig121614.4442.1 ID=mRNA_M-pyrifera_M_contig121614.4442.1|Name=mRNA_M-pyrifera_M_contig121614.4442.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=531bp|location=Sequence derived from alignment at M-pyrifera_M_contig121614:2..532+ (Macrocystis pyrifera P11B4 male)
CTCGACATCGCCGAGCGCATCGCAACGATCACGCTGAACCGGCCCGACGC
CCGCAATGCCCTCTCGAGCGACGTGCTGCGCAAGGTGCCCGCCGCCATGC
TGCGCGCCGAATCGGACGACGACGTCGACGTCATCATCCTCACCGGCACG
GATCCGGCGTTCACCGCCGGCCTGGACCTGAAGGAACTGGGGACGAGCGG
GTCGATGCTCACCGAGGGCACCGGCGCCGACGGCGGAGTGAACGCCTTCG
GCGCACGAGGCCCGTTCCCCCCACTCGAGAAGCCGCTGATCGGAGCCGTG
AACGGTGTCGCCGTCACCGGCGGGCTCGAGTTCGCGTTGGCGTGCGACTT
TCTCGTGGCGAGCGAGCGCGCGAAGTTCGGCGACACTCATGCGCGTGTCG
GGGTGATGCCCGGGTGGGGTCTCACGGTGCTGCTCCCGCAGGCGATCGGC
GTGCGACGCGCTCGCGAGATGAGCTTCACGGGCAATTTCCTGCCCGCGGA
GGAGGCACTCCGCTGCGGTCTCGTCAACCAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig121614:2..532+

>mRNA_M-pyrifera_M_contig121614.4442.1 ID=mRNA_M-pyrifera_M_contig121614.4442.1|Name=mRNA_M-pyrifera_M_contig121614.4442.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1062bp|location=Sequence derived from alignment at M-pyrifera_M_contig121614:2..532+ (Macrocystis pyrifera P11B4 male)
CTCGACATCGCCGAGCGCATCGCAACGATCACGCTGAACCGGCCCGACGC
CCGCAATGCCCTCTCGAGCGACGTGCTGCGCAAGGTGCCCGCCGCCATGC
TGCGCGCCGAATCGGACGACGACGTCGACGTCATCATCCTCACCGGCACG
GATCCGGCGTTCACCGCCGGCCTGGACCTGAAGGAACTGGGGACGAGCGG
GTCGATGCTCACCGAGGGCACCGGCGCCGACGGCGGAGTGAACGCCTTCG
GCGCACGAGGCCCGTTCCCCCCACTCGAGAAGCCGCTGATCGGAGCCGTG
AACGGTGTCGCCGTCACCGGCGGGCTCGAGTTCGCGTTGGCGTGCGACTT
TCTCGTGGCGAGCGAGCGCGCGAAGTTCGGCGACACTCATGCGCGTGTCG
GGGTGATGCCCGGGTGGGGTCTCACGGTGCTGCTCCCGCAGGCGATCGGC
GTGCGACGCGCTCGCGAGATGAGCTTCACGGGCAATTTCCTGCCCGCGGA
GGAGGCACTCCGCTGCGGTCTCGTCAACCATCTCGACATCGCCGAGCGCA
TCGCAACGATCACGCTGAACCGGCCCGACGCCCGCAATGCCCTCTCGAGC
GACGTGCTGCGCAAGGTGCCCGCCGCCATGCTGCGCGCCGAATCGGACGA
CGACGTCGACGTCATCATCCTCACCGGCACGGATCCGGCGTTCACCGCCG
GCCTGGACCTGAAGGAACTGGGGACGAGCGGGTCGATGCTCACCGAGGGC
ACCGGCGCCGACGGCGGAGTGAACGCCTTCGGCGCACGAGGCCCGTTCCC
CCCACTCGAGAAGCCGCTGATCGGAGCCGTGAACGGTGTCGCCGTCACCG
GCGGGCTCGAGTTCGCGTTGGCGTGCGACTTTCTCGTGGCGAGCGAGCGC
GCGAAGTTCGGCGACACTCATGCGCGTGTCGGGGTGATGCCCGGGTGGGG
TCTCACGGTGCTGCTCCCGCAGGCGATCGGCGTGCGACGCGCTCGCGAGA
TGAGCTTCACGGGCAATTTCCTGCCCGCGGAGGAGGCACTCCGCTGCGGT
CTCGTCAACCAT
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