mRNA_M-pyrifera_M_contig120590.4241.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig120590.4241.1
Unique NamemRNA_M-pyrifera_M_contig120590.4241.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: A0A2P6NMU9_9EUKA (Uncharacterized protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NMU9_9EUKA)

HSP 1 Score: 132 bits (333), Expect = 1.260e-33
Identity = 62/112 (55.36%), Postives = 78/112 (69.64%), Query Frame = 1 
Query:   10 SQEDIETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDKGGAPFFKK 345
            S E+++ L     EIL  ++ H  SWPFH PV  ++VPDY + IKDPIDM+MI++R   G+YYITK IF AD+KRMCDNCR YNR DT +FKCAN IE       GA +FK+
Sbjct:  692 SPEELDRLFRDMREILVLMRDHPSSWPFHHPVDRNQVPDYEDHIKDPIDMEMIERRLKRGNYYITKEIFLADVKRMCDNCRYYNRPDTEYFKCANEIENEFVK--GAKYFKR 801
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: L8GSZ1_ACACA (Histone acetyltransferase (Fragment) n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GSZ1_ACACA)

HSP 1 Score: 126 bits (316), Expect = 2.380e-31
Identity = 51/107 (47.66%), Postives = 79/107 (73.83%), Query Frame = 1 
Query:   13 QEDIETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDKGGAP 333
            +E +E+L V F ++L++I+ H  +WPF +PV  +EV DYY++I+DPID++ ++KR DTG +YIT+ IF ADL+RMC NCR YN  + V++ CA TI++ + +   AP
Sbjct:  608 RESMESLQVVFRDVLRSIKEHPSAWPFLQPVDKNEVWDYYDIIRDPIDLETMEKRLDTGQFYITREIFIADLRRMCQNCRRYNGPNNVYYDCAETIDRFVEEVFSAP 714
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: UPI001C4AA98C (histone acetyltransferase GCN5-like isoform X3 n=1 Tax=Zingiber officinale TaxID=94328 RepID=UPI001C4AA98C)

HSP 1 Score: 118 bits (295), Expect = 5.910e-31
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 1 
Query:   52 ILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDKGGA 330
            +LK +  H D+WPF EPV   EVPDYY++IKDP+D++ + KR ++G YY+T  +F AD+KRMC N RTYN  +T++FKCAN +E     K  A
Sbjct:  107 LLKLMFEHPDAWPFKEPVDAREVPDYYDIIKDPMDLRTMSKRLESGQYYVTFEMFVADVKRMCGNARTYNSPETIYFKCANRLENFFTSKAQA 199
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: L8GG22_ACACA (Bromodomain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GG22_ACACA)

HSP 1 Score: 124 bits (311), Expect = 1.200e-30
Identity = 55/115 (47.83%), Postives = 81/115 (70.43%), Query Frame = 1 
Query:    4 ERSQEDIETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDKGGAPFFKKK 348
            ++ +E+IE LH    + LK+IQ+H  +WPF +PVS  E P Y+E+IKDPID++ ++KR   G YYIT+ IF ADL+RM  NCRTYN  +TV++KCA+ ++K + +    P  KK+
Sbjct:  754 QQEEENIEYLHEVLKQQLKSIQAHASAWPFLKPVSRKEAPHYHEIIKDPIDLETMEKRLAAGQYYITQDIFIADLRRMFANCRTYNAPETVYYKCADQLDKYVDELFHLPPAKKR 868
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: F0Z8L9_DICPU (Histone acetyltransferase n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0Z8L9_DICPU)

HSP 1 Score: 120 bits (302), Expect = 1.340e-30
Identity = 51/96 (53.12%), Postives = 69/96 (71.88%), Query Frame = 1 
Query:   22 IETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKL 309
            I+ L +  G+ L  I++H DSWPF + VS DE P+YY V+KDP+D+QMI  R    +YYITK+IF ADLKRMC+NCR +N   TV+F+ A+ +EK 
Sbjct:  255 IQNLQIQLGKALVEIKNHEDSWPFQKAVSLDEAPNYYNVVKDPVDLQMISDRLAANNYYITKNIFLADLKRMCNNCREFNGEGTVYFEVADRLEKF 350
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: A0A2P6NBE7_9EUKA (Histone acetyltransferase n=2 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NBE7_9EUKA)

HSP 1 Score: 124 bits (310), Expect = 1.650e-30
Identity = 55/105 (52.38%), Postives = 72/105 (68.57%), Query Frame = 1 
Query:   19 DIETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDKGGAP 333
            +I+ L     E++  ++ H +SWPFH PV   EVPDYY++I DPID++MI+KRF  G+YYITK IF AD+KRMCDNCR YNR DT +FK     +   C+K   P
Sbjct:  769 EIDLLMREVREVIIKLKEHPNSWPFHHPVDRKEVPDYYQIISDPIDLEMIEKRFKRGNYYITKEIFLADVKRMCDNCRVYNREDTDYFKLT---QYKYCEKTQIP 870
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: UPI001C4AE8BE (histone acetyltransferase GCN5-like isoform X1 n=2 Tax=Zingiber officinale TaxID=94328 RepID=UPI001C4AE8BE)

HSP 1 Score: 118 bits (295), Expect = 4.270e-30
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 1 
Query:   52 ILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDKGGA 330
            +LK +  H D+WPF EPV   EVPDYY++IKDP+D++ + KR ++G YY+T  +F AD+KRMC N RTYN  +T++FKCAN +E     K  A
Sbjct:  192 LLKLMFEHPDAWPFKEPVDAREVPDYYDIIKDPMDLRTMSKRLESGQYYVTFEMFVADVKRMCGNARTYNSPETIYFKCANRLENFFTSKAQA 284
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: A0A151ZGQ8_9MYCE (Histone acetyltransferase n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151ZGQ8_9MYCE)

HSP 1 Score: 119 bits (298), Expect = 1.160e-29
Identity = 47/100 (47.00%), Postives = 75/100 (75.00%), Query Frame = 1 
Query:   22 IETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDK 321
            I+ L + F +IL+ +++H D+WPF +PVS D+ P+YY ++KDP+D+Q I +R     YYIT++IF ADLKRMC+NCR +N+  +V+++ A+ +EK I D+
Sbjct:  307 IQQLQIKFEKILELVKAHPDAWPFLQPVSIDDAPNYYNIVKDPVDLQKIAERLALNQYYITENIFQADLKRMCNNCREFNQEGSVYYEIADRLEKFIVDQ 406
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: Q54R05_DICDI (Histone acetyltransferase n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=Q54R05_DICDI)

HSP 1 Score: 119 bits (297), Expect = 1.590e-29
Identity = 50/101 (49.50%), Postives = 74/101 (73.27%), Query Frame = 1 
Query:    4 ERSQEDIETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEK 306
            ++ Q+ I +L      +L++I++H DSWPF +PVS +EVP YY  +KDP+D+QMI  R  TG+YYITK+IF ADLKRMC+NCR +N  ++ ++  A+ +EK
Sbjct:  301 DQQQQQINSLQQQLAVVLQSIKNHDDSWPFLQPVSIEEVPTYYTTVKDPVDLQMISDRLATGNYYITKNIFLADLKRMCNNCREFNGENSPYYDNADRLEK 401
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BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Match: A0A0A9KV31_ARUDO (GCN5 n=2 Tax=Arundo donax TaxID=35708 RepID=A0A0A9KV31_ARUDO)

HSP 1 Score: 109 bits (273), Expect = 6.990e-29
Identity = 43/90 (47.78%), Postives = 64/90 (71.11%), Query Frame = 1 
Query:   52 ILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDPIDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTIEKLICDK 321
            +LK++  H D+WPF EPV   +VPDYY++IKDPID++ + +R ++  YY+T  +F AD+KRM +N RTYN  DT+++KCA  +E     K
Sbjct:    4 LLKSMNEHPDAWPFKEPVDSRDVPDYYDIIKDPIDLRTMSRRVESEQYYVTLEMFVADMKRMFNNARTYNSPDTIYYKCATRLENFFSGK 93
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig120590.4241.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2P6NMU9_9EUKA1.260e-3355.36Uncharacterized protein n=1 Tax=Planoprotostelium ... [more]
L8GSZ1_ACACA2.380e-3147.66Histone acetyltransferase (Fragment) n=1 Tax=Acant... [more]
UPI001C4AA98C5.910e-3151.61histone acetyltransferase GCN5-like isoform X3 n=1... [more]
L8GG22_ACACA1.200e-3047.83Bromodomain containing protein n=1 Tax=Acanthamoeb... [more]
F0Z8L9_DICPU1.340e-3053.13Histone acetyltransferase n=1 Tax=Dictyostelium pu... [more]
A0A2P6NBE7_9EUKA1.650e-3052.38Histone acetyltransferase n=2 Tax=Planoprotosteliu... [more]
UPI001C4AE8BE4.270e-3051.61histone acetyltransferase GCN5-like isoform X1 n=2... [more]
A0A151ZGQ8_9MYCE1.160e-2947.00Histone acetyltransferase n=1 Tax=Tieghemostelium ... [more]
Q54R05_DICDI1.590e-2949.50Histone acetyltransferase n=1 Tax=Dictyostelium di... [more]
A0A0A9KV31_ARUDO6.990e-2947.78GCN5 n=2 Tax=Arundo donax TaxID=35708 RepID=A0A0A9... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig120590contigM-pyrifera_M_contig120590:133..538 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score120.9
Seed ortholog evalue3.9e-25
Seed eggNOG ortholog5786.XP_003283697.1
Preferred nameHAG1
KEGG koko:K06062
KEGG Pathwayko04330,ko04919,ko05166,ko05203,map04330,map04919,map05166,map05203
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000123,GO:0000428,GO:0001816,GO:0002682,GO:0002694,GO:0003006,GO:0003008,GO:0003674,GO:0003676,GO:0003677,GO:0003682,GO:0003712,GO:0003713,GO:0003824,GO:0004402,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005667,GO:0005671,GO:0005813,GO:0005815,GO:0005856,GO:0006139,GO:0006325,GO:0006338,GO:0006351,GO:0006355,GO:0006357,GO:0006366,GO:0006464,GO:0006473,GO:0006475,GO:0006508,GO:0006725,GO:0006807,GO:0006996,GO:0007275,GO:0007507,GO:0007610,GO:0007611,GO:0007613,GO:0007616,GO:0008080,GO:0008134,GO:0008150,GO:0008152,GO:0008233,GO:0008234,GO:0009058,GO:0009059,GO:0009314,GO:0009416,GO:0009628,GO:0009653,GO:0009791,GO:0009889,GO:0009891,GO:0009893,GO:0009908,GO:0009987,GO:0010015,GO:0010321,GO:0010467,GO:0010468,GO:0010484,GO:0010556,GO:0010557,GO:0010564,GO:0010604,GO:0010628,GO:0010639,GO:0010646,GO:0010824,GO:0010826,GO:0010948,GO:0015630,GO:0016043,GO:0016070,GO:0016407,GO:0016410,GO:0016569,GO:0016570,GO:0016573,GO:0016578,GO:0016579,GO:0016591,GO:0016740,GO:0016746,GO:0016747,GO:0016787,GO:0018130,GO:0018193,GO:0018205,GO:0018335,GO:0018393,GO:0018394,GO:0019219,GO:0019222,GO:0019438,GO:0019538,GO:0019783,GO:0019899,GO:0022414,GO:0022622,GO:0023051,GO:0030880,GO:0030914,GO:0031248,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031647,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0032774,GO:0032886,GO:0032991,GO:0033043,GO:0033276,GO:0034212,GO:0034641,GO:0034645,GO:0034654,GO:0036211,GO:0036459,GO:0040029,GO:0042826,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0043543,GO:0043966,GO:0044087,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044430,GO:0044446,GO:0044451,GO:0044464,GO:0044798,GO:0045580,GO:0045589,GO:0045595,GO:0045619,GO:0045786,GO:0045815,GO:0045893,GO:0045935,GO:0045944,GO:0046483,GO:0046599,GO:0046600,GO:0046605,GO:0046606,GO:0048167,GO:0048364,GO:0048367,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048580,GO:0048608,GO:0048731,GO:0048736,GO:0048856,GO:0050789,GO:0050793,GO:0050794,GO:0050804,GO:0050863,GO:0050865,GO:0050877,GO:0050890,GO:0050896,GO:0051128,GO:0051129,GO:0051171,GO:0051173,GO:0051239,GO:0051249,GO:0051252,GO:0051254,GO:0051276,GO:0051493,GO:0051494,GO:0051726,GO:0055029,GO:0060173,GO:0060255,GO:0061458,GO:0061695,GO:0061733,GO:0065007,GO:0065008,GO:0070011,GO:0070013,GO:0070461,GO:0070507,GO:0070646,GO:0070647,GO:0071704,GO:0071840,GO:0072359,GO:0080090,GO:0090304,GO:0090567,GO:0090575,GO:0097159,GO:0097659,GO:0099177,GO:0099402,GO:0101005,GO:0106075,GO:0106077,GO:0106078,GO:0140096,GO:0140110,GO:1901360,GO:1901362,GO:1901363,GO:1901564,GO:1901576,GO:1902105,GO:1902115,GO:1902116,GO:1902493,GO:1902494,GO:1902562,GO:1902680,GO:1903506,GO:1903508,GO:1903706,GO:1905392,GO:1990234,GO:2000026,GO:2000112,GO:2001141
EggNOG free text desc.Acetyltransferase (GNAT) domain
EggNOG OGs3X8MU@554915,COG5076@1,KOG1472@2759
EC2.3.1.48
COG Functional cat.BK
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03021,ko03036
Exons2
Model size351
Cds size351
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815725.5294738-CDS-M-pyrifera_M_contig120590:132..3291622815725.5294738-CDS-M-pyrifera_M_contig120590:132..329Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120590 133..329 -
1692277540.0621943-CDS-M-pyrifera_M_contig120590:132..3291692277540.0621943-CDS-M-pyrifera_M_contig120590:132..329Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120590 133..329 -
1622815725.546403-CDS-M-pyrifera_M_contig120590:384..5381622815725.546403-CDS-M-pyrifera_M_contig120590:384..538Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120590 385..538 -
1692277540.0765135-CDS-M-pyrifera_M_contig120590:384..5381692277540.0765135-CDS-M-pyrifera_M_contig120590:384..538Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120590 385..538 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig120590.4241.1prot_M-pyrifera_M_contig120590.4241.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig120590 133..538 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig120590.4241.1

>prot_M-pyrifera_M_contig120590.4241.1 ID=prot_M-pyrifera_M_contig120590.4241.1|Name=mRNA_M-pyrifera_M_contig120590.4241.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=117bp
EERSQEDIETLHVAFGEILKAIQSHTDSWPFHEPVSFDEVPDYYEVIKDP
IDMQMIQKRFDTGSYYITKHIFFADLKRMCDNCRTYNRNDTVFFKCANTI
EKLICDKGGAPFFKKK*
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mRNA from alignment at M-pyrifera_M_contig120590:133..538-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig120590.4241.1 ID=mRNA_M-pyrifera_M_contig120590.4241.1|Name=mRNA_M-pyrifera_M_contig120590.4241.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=406bp|location=Sequence derived from alignment at M-pyrifera_M_contig120590:133..538- (Macrocystis pyrifera P11B4 male)
GAGGAGCGCAGCCAGGAGGATATTGAGACCCTCCACGTCGCCTTTGGAGA
GATTCTCAAGGCGATCCAGAGCCACACAGACTCCTGGCCCTTCCACGAGC
CGGTGAGCTTCGACGAGGTGCCGGACTACTACGAAGTGATCAAGGACCCC
ATTGGTACGTTGGCTCTTTGCAGAGGGGTACTTTACGCTTGTCGCTGACA
ACTGTGCAGATATGCAGATGATCCAGAAGCGGTTCGACACAGGCAGCTAC
TACATCACCAAGCACATCTTCTTCGCGGACCTGAAGAGGATGTGCGATAA
CTGCCGAACGTACAACCGCAACGACACGGTGTTCTTCAAGTGCGCAAACA
CCATCGAGAAGCTGATCTGCGACAAGGGGGGCGCACCCTTCTTCAAGAAG
AAGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig120590:133..538-

>mRNA_M-pyrifera_M_contig120590.4241.1 ID=mRNA_M-pyrifera_M_contig120590.4241.1|Name=mRNA_M-pyrifera_M_contig120590.4241.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=702bp|location=Sequence derived from alignment at M-pyrifera_M_contig120590:133..538- (Macrocystis pyrifera P11B4 male)
GAGGAGCGCAGCCAGGAGGATATTGAGACCCTCCACGTCGCCTTTGGAGA
GATTCTCAAGGCGATCCAGAGCCACACAGACTCCTGGCCCTTCCACGAGC
CGGTGAGCTTCGACGAGGTGCCGGACTACTACGAAGTGATCAAGGACCCC
ATTGGAGGAGCGCAGCCAGGAGGATATTGAGACCCTCCACGTCGCCTTTG
GAGAGATTCTCAAGGCGATCCAGAGCCACACAGACTCCTGGCCCTTCCAC
GAGCCGGTGAGCTTCGACGAGGTGCCGGACTACTACGAAGTGATCAAGGA
CCCCATTGATATGCAGATGATCCAGAAGCGGTTCGACACAGGCAGCTACT
ACATCACCAAGCACATCTTCTTCGCGGACCTGAAGAGGATGTGCGATAAC
TGCCGAACGTACAACCGCAACGACACGGTGTTCTTCAAGTGCGCAAACAC
CATCGAGAAGCTGATCTGCGACAAGGGGGGCGCACCCTTCTTCAAGAAGA
AGTAAATATGCAGATGATCCAGAAGCGGTTCGACACAGGCAGCTACTACA
TCACCAAGCACATCTTCTTCGCGGACCTGAAGAGGATGTGCGATAACTGC
CGAACGTACAACCGCAACGACACGGTGTTCTTCAAGTGCGCAAACACCAT
CGAGAAGCTGATCTGCGACAAGGGGGGCGCACCCTTCTTCAAGAAGAAGT
AA
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