mRNA_M-pyrifera_M_contig118893.3880.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig118893.3880.1
Unique NamemRNA_M-pyrifera_M_contig118893.3880.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A3L6JHJ9_THOAR (Glucosylceramidase n=1 Tax=Thorarchaeota archaeon (strain OWC) TaxID=2053491 RepID=A0A3L6JHJ9_THOAR)

HSP 1 Score: 82.8 bits (203), Expect = 7.350e-17
Identity = 39/66 (59.09%), Postives = 47/66 (71.21%), Query Frame = 1 
Query:   10 LSGWAW--KKVQGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIEN 201
            L  WAW  K  +  YH LFPRAWTTYD    D  ++V+C+Q+SPVIA NYKE+S+PV VFAW I N
Sbjct:  134 LKSWAWGLKPNRAEYHALFPRAWTTYDFS--DLGIEVVCQQISPVIAHNYKESSYPVGVFAWRIRN 197
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BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A446T8G0_TRITD (Glyco_hydr_116N domain-containing protein n=1 Tax=Triticum turgidum subsp. durum TaxID=4567 RepID=A0A446T8G0_TRITD)

HSP 1 Score: 78.6 bits (192), Expect = 2.920e-16
Identity = 34/70 (48.57%), Postives = 48/70 (68.57%), Query Frame = 1 
Query:    1 KETLSGWAWKKVQG--TYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENT 204
            K T+  W WK  +   TYH LFPR+WT YD  + D  +K+ C+Q+SP+I  NYKE+SFPV VF + ++N+
Sbjct:  107 KATIGSWDWKLKEKNCTYHALFPRSWTVYD-GEPDPEIKITCRQISPIIPHNYKESSFPVAVFTFTVQNS 175
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BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A446T8E8_TRITD (Glyco_hydr_116N domain-containing protein n=1 Tax=Triticum turgidum subsp. durum TaxID=4567 RepID=A0A446T8E8_TRITD)

HSP 1 Score: 78.6 bits (192), Expect = 3.130e-16
Identity = 34/70 (48.57%), Postives = 48/70 (68.57%), Query Frame = 1 
Query:    1 KETLSGWAWKKVQG--TYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENT 204
            K T+  W WK  +   TYH LFPR+WT YD  + D  +K+ C+Q+SP+I  NYKE+SFPV VF + ++N+
Sbjct:   97 KATIGSWDWKLKEKNCTYHALFPRSWTVYD-GEPDPEIKITCRQISPIIPHNYKESSFPVAVFTFTVQNS 165
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A446T8G8_TRITD (Glyco_hydr_116N domain-containing protein n=6 Tax=Triticum turgidum subsp. durum TaxID=4567 RepID=A0A446T8G8_TRITD)

HSP 1 Score: 78.6 bits (192), Expect = 6.270e-16
Identity = 34/70 (48.57%), Postives = 48/70 (68.57%), Query Frame = 1 
Query:    1 KETLSGWAWKKVQG--TYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENT 204
            K T+  W WK  +   TYH LFPR+WT YD  + D  +K+ C+Q+SP+I  NYKE+SFPV VF + ++N+
Sbjct:  153 KATIGSWDWKLKEKNCTYHALFPRSWTVYD-GEPDPEIKITCRQISPIIPHNYKESSFPVAVFTFTVQNS 221
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: M8AIT3_AEGTA (Glyco_hydr_116N domain-containing protein n=1 Tax=Aegilops tauschii TaxID=37682 RepID=M8AIT3_AEGTA)

HSP 1 Score: 79.0 bits (193), Expect = 1.350e-15
Identity = 35/73 (47.95%), Postives = 49/73 (67.12%), Query Frame = 1 
Query:    1 KETLSGWAWKKVQG--TYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENTHPT 213
            K T+  W WK  +   TYH LFPR+WT YD  + D  +K+ C+Q+SP+I  NYKE+SFPV VF + ++N+  T
Sbjct:  128 KATIGSWDWKLKERNCTYHALFPRSWTVYD-GEPDPEIKITCRQISPIIPHNYKESSFPVAVFTFTVQNSGST 199
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: D8RWH2_SELML (Non-lysosomal glucosylceramidase n=5 Tax=Selaginella moellendorffii TaxID=88036 RepID=D8RWH2_SELML)

HSP 1 Score: 78.2 bits (191), Expect = 3.110e-15
Identity = 36/67 (53.73%), Postives = 48/67 (71.64%), Query Frame = 1 
Query:   10 LSGWAWKK--VQGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENT 204
            +S W W    V+ TY+GLFPRAWTTYD  + D ++K+ C+QVSP +  NYKE+SFP  VF +E+ NT
Sbjct:  145 ISSWNWNLTGVKSTYYGLFPRAWTTYD-GEPDPQMKITCRQVSPFLPHNYKESSFPSCVFIYELVNT 210
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BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A6A4WG28_AMPAM (Non-lysosomal glucosylceramidase n=5 Tax=Amphibalanus amphitrite TaxID=1232801 RepID=A0A6A4WG28_AMPAM)

HSP 1 Score: 77.8 bits (190), Expect = 4.230e-15
Identity = 36/67 (53.73%), Postives = 45/67 (67.16%), Query Frame = 1 
Query:   10 LSGWAWK--KVQGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENT 204
            LSGW W     Q  Y GL+PRAWT Y +   + RL++ C+QVSPVI  NYK+TS P  VF W++ENT
Sbjct:  132 LSGWRWSFPGAQAEYRGLYPRAWTDYRLP--EQRLRLGCRQVSPVIPHNYKDTSLPCAVFVWDVENT 196
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: UPI00062655EA (non-lysosomal glucosylceramidase n=1 Tax=Athalia rosae TaxID=37344 RepID=UPI00062655EA)

HSP 1 Score: 77.8 bits (190), Expect = 4.230e-15
Identity = 35/66 (53.03%), Postives = 45/66 (68.18%), Query Frame = 1 
Query:   10 LSGWAW--KKVQGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIEN 201
            L  W W     + TY GLFPRAWTTYD+   +  +K++C+QVSP+I  NYK++S P  VF WEIEN
Sbjct:  165 LKSWKWGFDGSKATYTGLFPRAWTTYDID--EHNVKLICRQVSPIIPNNYKDSSLPCAVFIWEIEN 228
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A7C5A7E0_9CHLR (Uncharacterized protein n=1 Tax=Anaerolineae bacterium TaxID=2052143 RepID=A0A7C5A7E0_9CHLR)

HSP 1 Score: 77.4 bits (189), Expect = 5.780e-15
Identity = 37/69 (53.62%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:    7 TLSGWAWKKVQGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENTHPT 213
            T  GW     +GTY+ LFPRAWT Y+      RL   C+Q+SPVI  NY+E+SFPV VF W IENT  T
Sbjct:  141 TAWGWGLPAAKGTYYALFPRAWTVYEAPLPGLRLT--CRQISPVIPHNYRESSFPVGVFVWTIENTADT 207
[Close]
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Match: A0A6J1IED1_CUCMA (Non-lysosomal glucosylceramidase n=9 Tax=Cucurbita TaxID=3660 RepID=A0A6J1IED1_CUCMA)

HSP 1 Score: 77.4 bits (189), Expect = 5.800e-15
Identity = 35/70 (50.00%), Postives = 47/70 (67.14%), Query Frame = 1 
Query:   10 LSGWAWKKV--QGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQNYKETSFPVTVFAWEIENTHPT 213
            +  W W     + TYH LFPRAWT YD  + D  LK++C+Q+SP+I  NYKE+SFPV+VF + + N  PT
Sbjct:  160 IEAWDWNLSGEKSTYHALFPRAWTIYD-GEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPT 228
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig118893.3880.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3L6JHJ9_THOAR7.350e-1759.09Glucosylceramidase n=1 Tax=Thorarchaeota archaeon ... [more]
A0A446T8G0_TRITD2.920e-1648.57Glyco_hydr_116N domain-containing protein n=1 Tax=... [more]
A0A446T8E8_TRITD3.130e-1648.57Glyco_hydr_116N domain-containing protein n=1 Tax=... [more]
A0A446T8G8_TRITD6.270e-1648.57Glyco_hydr_116N domain-containing protein n=6 Tax=... [more]
M8AIT3_AEGTA1.350e-1547.95Glyco_hydr_116N domain-containing protein n=1 Tax=... [more]
D8RWH2_SELML3.110e-1553.73Non-lysosomal glucosylceramidase n=5 Tax=Selaginel... [more]
A0A6A4WG28_AMPAM4.230e-1553.73Non-lysosomal glucosylceramidase n=5 Tax=Amphibala... [more]
UPI00062655EA4.230e-1553.03non-lysosomal glucosylceramidase n=1 Tax=Athalia r... [more]
A0A7C5A7E0_9CHLR5.780e-1553.62Uncharacterized protein n=1 Tax=Anaerolineae bacte... [more]
A0A6J1IED1_CUCMA5.800e-1550.00Non-lysosomal glucosylceramidase n=9 Tax=Cucurbita... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig118893contigM-pyrifera_M_contig118893:332..544 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score79.7
Seed ortholog evalue6e-13
Seed eggNOG ortholog37682.EMT04371
KEGG rclassRC00059,RC00451
KEGG koko:K17108
KEGG ReactionR01498
KEGG Pathwayko00511,ko00600,ko01100,map00511,map00600,map01100
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004348,GO:0004553,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005790,GO:0005886,GO:0006082,GO:0006629,GO:0006643,GO:0006664,GO:0006665,GO:0006687,GO:0006807,GO:0007275,GO:0007399,GO:0007417,GO:0008150,GO:0008152,GO:0008202,GO:0008206,GO:0008422,GO:0009056,GO:0009987,GO:0012505,GO:0015926,GO:0016020,GO:0016021,GO:0016137,GO:0016139,GO:0016787,GO:0016798,GO:0019752,GO:0021953,GO:0021954,GO:0022008,GO:0030154,GO:0030182,GO:0031224,GO:0032501,GO:0032502,GO:0032787,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044255,GO:0044281,GO:0044424,GO:0044425,GO:0044444,GO:0044464,GO:0048468,GO:0048666,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0071704,GO:0071944,GO:1901135,GO:1901136,GO:1901360,GO:1901564,GO:1901575,GO:1901615,GO:1901657,GO:1901658,GO:1903509
EggNOG free text desc.Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide
EggNOG OGs37JKE@33090,3GG94@35493,3I7VT@38820,3KPX4@4447,COG4354@1,KOG2119@2759
EC3.2.1.45
COG Functional cat.G
CAZyGH116
Best tax levelPoales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size213
Cds size213
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815707.642189-CDS-M-pyrifera_M_contig118893:331..5441622815707.642189-CDS-M-pyrifera_M_contig118893:331..544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig118893 332..544 +
1692277531.8294244-CDS-M-pyrifera_M_contig118893:331..5441692277531.8294244-CDS-M-pyrifera_M_contig118893:331..544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig118893 332..544 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig118893.3880.1prot_M-pyrifera_M_contig118893.3880.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig118893 332..544 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig118893.3880.1

>prot_M-pyrifera_M_contig118893.3880.1 ID=prot_M-pyrifera_M_contig118893.3880.1|Name=mRNA_M-pyrifera_M_contig118893.3880.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=71bp
KETLSGWAWKKVQGTYHGLFPRAWTTYDVHKMDSRLKVLCKQVSPVIAQN
YKETSFPVTVFAWEIENTHPT
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mRNA from alignment at M-pyrifera_M_contig118893:332..544+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig118893.3880.1 ID=mRNA_M-pyrifera_M_contig118893.3880.1|Name=mRNA_M-pyrifera_M_contig118893.3880.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=213bp|location=Sequence derived from alignment at M-pyrifera_M_contig118893:332..544+ (Macrocystis pyrifera P11B4 male)
AAGGAGACACTGAGTGGCTGGGCTTGGAAGAAGGTGCAGGGGACTTACCA
CGGGCTGTTCCCTCGCGCCTGGACGACGTACGACGTCCACAAGATGGACA
GCAGGCTGAAGGTGCTGTGCAAACAGGTGTCGCCTGTGATTGCGCAGAAC
TACAAGGAGACCAGCTTTCCTGTGACTGTGTTTGCTTGGGAGATCGAGAA
CACGCACCCTACG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig118893:332..544+

>mRNA_M-pyrifera_M_contig118893.3880.1 ID=mRNA_M-pyrifera_M_contig118893.3880.1|Name=mRNA_M-pyrifera_M_contig118893.3880.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=426bp|location=Sequence derived from alignment at M-pyrifera_M_contig118893:332..544+ (Macrocystis pyrifera P11B4 male)
AAGGAGACACTGAGTGGCTGGGCTTGGAAGAAGGTGCAGGGGACTTACCA
CGGGCTGTTCCCTCGCGCCTGGACGACGTACGACGTCCACAAGATGGACA
GCAGGCTGAAGGTGCTGTGCAAACAGGTGTCGCCTGTGATTGCGCAGAAC
TACAAGGAGACCAGCTTTCCTGTGACTGTGTTTGCTTGGGAGATCGAGAA
CACGCACCCTACGAAGGAGACACTGAGTGGCTGGGCTTGGAAGAAGGTGC
AGGGGACTTACCACGGGCTGTTCCCTCGCGCCTGGACGACGTACGACGTC
CACAAGATGGACAGCAGGCTGAAGGTGCTGTGCAAACAGGTGTCGCCTGT
GATTGCGCAGAACTACAAGGAGACCAGCTTTCCTGTGACTGTGTTTGCTT
GGGAGATCGAGAACACGCACCCTACG
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