mRNA_M-pyrifera_M_contig11885.3873.1 (mRNA) Macrocystis pyrifera P11B4 male

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A6H5JET4_9PHAE (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JET4_9PHAE)

HSP 1 Score: 866 bits (2238), Expect = 4.200e-296
Identity = 502/1018 (49.31%), Postives = 616/1018 (60.51%), Query Frame = 1

Query:  292 DAGVLSQLPLFVQECFPFVLTRKSAIHVDVLLELSDNLVHSKGFAASSEALAQAHLKKFHDAELKYYNMALWRRHSALGPVATESRFGTFDAPDGYNDFFPSPHYLSTAWTEFMQERSDFMQQRHQHVDGVVMKIDASHKLVKLVRVYAPGAGADTKPTYGVITIFNEFEQVVFQKALQSASLREIKVDLKALFAIRYKGSGFKLPQVVYTDMCCEDRALFAEIVQALREEGHDLAVDGEISPPPAGPVLDLPPDVEVA-CIKASKETPGVAAAAQNIRQQAEAHGRVVGLDLEWEISRFGAPGNPPATIQLAAGKQAVIFHVLHGQRSSPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYGVDVKNVVDLPALALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQKR---------------LRFLSDPIFTDAKELNAGNYVRLYTRGGNDCVGEGRIIEYHKETWGTTGIFVSPRRSTRGLTRRWVVQLVTVHVHRAKTLYPDECDDRHTQVTAQVGDRKDQRLLWDAGRLRKVSVNTGVTTSESGHAGGRCATAGDRGGINPGLGSPALRPNGE--------EMVRCVDASGVTPGMLPHAPAAAVPLSTTGPGGGG---------------------------VTPSESFFSGDFSGL----TRDFWCSILSRGVQE--------------------------------------ARLCVKLDAFHGMQRLSKLILKNHGACRAFLARLRDAFFKVYAEDIAGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEG----------------LAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSMLFGEAEYSSWLPTSAYGDTGERNGFFRSVLPEGS-GGGTDFLDDLTIGGNES 3015
            ++ VL+ LPL+VQE FPF LTRKSAIH DV+ E++DNL+H+KGF+ASSEAL QAHL +FH  ELKYY+M LWRR   L     E  FG+F+  DGYN F PS HYLST W E MQ+      +  +HV+GV                                       +VV QKALQSASLRE+K DLKALF  RY G GFKLP++V TD CC D+AL  EI + L  EGH  +V   +      PVL LPPDV +   +KA++++P + AA   IR QAEA+  ++ LD+EWEIS  GAP NPPATIQLAAGK+ VIFHVLHGQRS P +LP  +V+ LED  + K GVGIK DCTRL++FYGV+V + VDL +LA+ RKV++GLRRGLADL  HLLG+ L K+  +R+S+WN+  LSDDQKR               +  LSD IF DA+EL+ G+ VRLYTR GNDCVGEG I+E+  ETWGTTG ++ PRR+TR   RRW V+L  VHV RAKTLYPD  D R T  TAQ+  R D  +LWDA RLRK +    +T   +G                                     E  R       T  +  H   + V     GPGGGG                           VTPSE FF G+  G+    +    CS  S G+ +                                      A L VKLD FH MQR+SKLILK+HGACRA+LARLRDAFFKV AED+A VEAAL +AGMS+EEV  KK  DW +FL+RCRR+IP   E L+R DLV  ++GGL+DKKTKQPL RA+A  A KRLR HI   C SD  + +LYF+ GK+  T  PL+RC+RGTNDLEG                LA LLLLE+NYRWNLRQ++KNRGLD +VGGFY+QP+LEAIQRMTS L+G+A+YS WLPTSAYGDTGER+GF RS+ P    GGGTD + ++ +GG E+
Sbjct:    6 NSDVLALLPLYVQESFPFFLTRKSAIHRDVVAEVADNLMHAKGFSASSEALQQAHLYRFHSMELKYYSMLLWRREEQLD-AGVEDEFGSFEDADGYNGFSPSEHYLSTVWCERMQQSR--AARLDKHVNGVQ-------------------------------------GEVVLQKALQSASLRELKQDLKALFITRYTGGGFKLPRLVSTDECCNDKALLNEIFEELASEGHQFSVTNSVPIASECPVLHLPPDVVLGEPVKANQDSPCLKAAVDAIRMQAEANNGLLALDIEWEISAAGAPANPPATIQLAAGKRVVIFHVLHGQRSVPAELPDPLVDLLEDTKLTKTGVGIKGDCTRLTSFYGVEVAHAVDLRSLAVERKVELGLRRGLADLCVHLLGQQLPKDPTVRLSKWNKLDLSDDQKRYAALDAYACLLLHGRIGELSDSIFRDAEELHVGDRVRLYTRAGNDCVGEGCIVEHTDETWGTTGFYLVPRRATRRFARRWAVRLDRVHVPRAKTLYPDATDPRGTPATAQLASRVDSVILWDASRLRKTTAI--LTVGAAGEPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEEARTAAXXXXTSSV--HVETSTVSCERGGPGGGGGKEGPRLPDSVGEADARRGAGQGGTEFVTPSEPFFCGELDGIEALSSESVACSAGSGGLADEGQAATAGGDDEEEDEVPLDVTADTSSAEVEELLFLKVAGLVVKLDTFHAMQRVSKLILKSHGACRAYLARLRDAFFKVNAEDLAAVEAALRQAGMSKEEVEAKKAKDWVYFLKRCRRLIPRPAELLERFDLVNNLYGGLIDKKTKQPLFRAEALEAIKRLRVHIMNDCCSDSPNEALYFEAGKDAETLCPLFRCIRGTNDLEGYHLHMRLLVAWCISPRLAHLLLLEYNYRWNLRQSMKNRGLDEAVGGFYDQPMLEAIQRMTSKLYGKAKYSQWLPTSAYGDTGERSGFVRSIFPSNVVGGGTDSVLEI-LGGAEN 978          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A6H5KEU8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEU8_9PHAE)

HSP 1 Score: 476 bits (1224), Expect = 1.060e-152
Identity = 290/591 (49.07%), Postives = 359/591 (60.74%), Query Frame = 1

Query: 1420 LALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQKR-LRFLSDPIFTDAKELNAGNYVRLYTRGGNDCVGEGRIIEYHKETWGTTGIFVSPRRSTRGLTRRWVVQLVTVHVHRAKTLYPDECDDRHTQVTAQVGDRKDQRLLWDAGRLRKVSVNTGVTTSESGHAGGRCATAGDRGGINPGLGSPALRPNGEEMVRCVDASGVTPGMLPHAPAAAVPLSTTG---------PGGGGVTPSESFF---------------------SGDF--SGLTRDFWCSILSRGVQEARLCVKLDAF----HGMQRL-----SKLILKNHGACRAFLARLRDAFFKVYAEDIAGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEG----------------LAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSMLFGEAEYSSWLPTSAYGDTGERNGFFRSVLP-EGSGGGTDFLDDLTIGGNES 3015
            +A+ RKV++  RRGLADL  +LL K L K+  +R+S+WN+  LSDDQKR +  LSD IF DA+E++ G+ VRLYTR GNDCVGEG I+E+  ETWGTTGI++ PRR+TRG  RRW V L  VHV RAK LYPD  D R    TAQ+  R D  +LWDA RLRK++      T  +G AG                          E  R       T     H  A+ V    +G          G GG+T                            GD   + +  D    + S  V+E  L +KL A     + + RL     SKLILK+HGACRA+LARLRD FFKV AED+A VEAAL +AGMS+EEV  KK  DW  FL+RCRR+IP   E L+R D+V  ++GGLLDKKTKQPL RA+A  A KRLR HI   C SD  + +LYF+ GK+  T  PL+RC+RGTNDLEG                LA LLLLE+NYRWNLRQ++KNRGLD +VGGFY+QP+LEAIQRMTS L+G+A+YS WLPTSAYGDTGER+GF RS+ P E  GGGTD + ++ +GG E+
Sbjct:    1 MAVERKVELWFRRGLADLCVNLLEKQLPKDPIVRLSKWNKLDLSDDQKRRIGELSDSIFRDAEEMHVGDSVRLYTRAGNDCVGEGCIVEHTDETWGTTGIYLVPRRATRGFARRWAVHLDRVHVPRAKALYPDATDRRGDPATAQLASRVDSVILWDASRLRKMTA-----TPSAGAAGAPXXXXXXX--XXXXXXXXXXXXXXXEAARAPAEEAAT--SSEHVEASTVSCERSGLRATNGGGKEGAGGLTDERQAXXXXXXXXXXXXXXXXXXATARGGDDEEAEVLLDVTADMSSAEVEEL-LFLKLKALDQRNYALLRLAAGEVSKLILKSHGACRAYLARLRDEFFKVNAEDLAAVEAALRQAGMSKEEVEAKKGKDWVLFLKRCRRLIPRPAELLKRFDVVNNLYGGLLDKKTKQPLFRAEALEAIKRLRVHIMNDCCSDAPNEALYFEAGKDAETLCPLFRCIRGTNDLEGFHLHMRLLVAWCISPRLAHLLLLEYNYRWNLRQSMKNRGLDEAVGGFYDQPILEAIQRMTSKLYGKAKYSQWLPTSAYGDTGERSGFVRSIFPSEVGGGGTDSVLEI-LGGAEN 580          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: D7FIV2_ECTSI (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIV2_ECTSI)

HSP 1 Score: 402 bits (1034), Expect = 5.480e-121
Identity = 318/983 (32.35%), Postives = 460/983 (46.80%), Query Frame = 1

Query:    7 WVHPQDPTMRKTLYV---GDHHLRSVFVWIPELTHPQSLPDGRPPCPRCKTAHRVIIKGWTQKLSRRAIMRDACCDLLGYFYKCQHCST---------RVTGTFQSWDAGVLSQLPLFVQECFPFVLTRKSAIHVDVLLELSDNLVHSKGFAASSEALAQAHLKKFHDAELKYYNMA--LWRRHSALGP---VATESRFGTFDAPDGYNDFFPSPHYLSTAWTEFMQE------------RSDFMQQRHQHVDGVVMKIDASHKLVKLVRVYAPGAGADTKPTYGVITIFNEFEQVVFQKALQSASLREIKVDLKALFAIRYKGSGFKLPQVVYTDMCCEDRAL----FAEIVQALREEGHDLAVDGEISPPPAG--PVLDLPPDVEVACI---KASKETPGVAAAAQNIRQQAEAHGRVVGLDLEWEISRFGAPGNPPATIQLAAGK-QAVIFHVLHG-QRSSPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYGVDVKNVVDLPALALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQKRLRFLSDPIFTDAKELNAGNYVRLYTRGGNDCVGEGRIIEYHKETWGTTGIFVSPRRSTRGLTRRWVVQLVTVHVHRAKTLYPD--ECDDRHTQVTAQVGDRKDQRLLWDAGRLRKVS----------VNTGVTTSESGHAGGRCATAGDRGGINPGLGSPALRPNGEEMVR----CVDASGVTPGMLPHAPAAAVPLSTTGPGGGGVTPSESFF----------------------SGDFSGLTRDFWCSILSRGVQEARLCVKLDAFHGMQRLSKLILKNHGACRAFLARLRDAFFKVYAEDIAGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLE 2721
            W HP+ P++ +   +   G  +L+ VFVW+PE    +       PCP C    R    GW  +  RR  + D  C L+GY Y C+ C+          R T TF +WDAGVL+ +  +V + FPFVLT+K+AI   V+  L+D+L+   GFAA+S++LA  +  ++ +    Y ++A     RH  + P   +    +F +   P G++   PS HYL   W +   E            R +++Q+R Q VDG  +  DAS K  K++R+ A   G   +P +G+ TI NE++QVVF KA+ +AS+ ++K DLK +F  R+ G GFKLP + Y+D CC+DR L    F EI        +D A +     P     P L+ PP     C+   + +   P V A     + Q    G V+GLD EWE S  G   NP +T+QL+     A  F +  G ++++    P ++   LE+ SIAKVGV I  D T L   YG++V N VDL   A    V+    R LA +++ LLG+ L K+  +R+SRW+   LSDDQ                      VRLY+     CVG G + +         G F   RR  +G   + VV L  V    A T +           +VT     + D+ +LWD   +R VS           ++    SE+ +   +  T+  R      L       +G+E       C D      G   H   A V     G GGG V  +                           GD   +  D W      G ++  L V+LD FH +QR+S+L  K+HGA + F+ARL+DA F V  +DI  VE AL   GM+ E VA  K   W +FL+ CRR++P     L+R + VI  F  ++D K+ + L+      A  +LR HI +  LSDP  V LY+  G+    G    RCVRGTN  E
Sbjct:   13 WAHPRLPSLHRGAGLPPLGPFYLKKVFVWVPEYLWDEI---EYIPCPHCNK--RAQPDGWNTE-PRRVFLEDDVCYLIGYRYLCKGCTEANKGRPEDERTTQTFNAWDAGVLATMDDYVSKEFPFVLTKKAAISKSVVDRLADDLLEGTGFAAASKSLANTYHARYFNQLRSYVSLANRCVSRHKGMWPNVDLGMIPKFSSIGDPSGFHSSPPSAHYLGDIWHKGFYETPVVQADGCSWTREEYLQRRAQLVDGCSLAGDASFKYAKVIRLSAGADGTRARPVHGIFTIMNEYDQVVFSKAMTTASVHDLKNDLKMMFVKRFLGHGFKLPVIFYSDECCDDRQLLLTMFKEIELETGARLYDTAEEAGGGSPGVEVLPALEFPPGKRGECVFDFRPNSIAPSVVALIDGCQLQ----GNVLGLDCEWEPSLGGTTPNPVSTVQLSLPDGTAYCFQLQRGNKKTTSSNFPKALQNLLENPSIAKVGVNINSDATYLERDYGIEVANTVDLRTYARQCWVETP-SRSLAGMASSLLGRQLPKDPVIRLSRWSSP-LSDDQ----------------------VRLYSNNHASCVGVGEVQDDDTAAEALAGWFPGARR--KGRLTQVVVGLSGVFKPAAYTAHKGAPRAASGVGRVTMDEAYKGDRLVLWDVAHVRLVSDWKPSPAAAAPSSNENASEAYNDNAQFFTS-PRPPHVVRLQDDEDPDDGDEEEYDGHDCGDLGSSARGD--HGLWAGVEAG--GGGGGAVNGTXXXXXXXXXXXXXXXXXXXXXXXXXXGGD---VDMDDWL-----GDEQCALQVRLDIFHALQRISRLCKKSHGAFKPFMARLKDACFIVNQDDIKEVEQALGSKGMTPEAVAEYKDKSWTYFLRNCRRLVPERQRLLERFNSVINQFCNVVDAKSGEVLILPKVMQAVDQLRKHIESDRLSDPHGVPLYYTTGQN-AAGITTRRCVRGTNCTE 945          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: D7FIV1_ECTSI (Similar to Werner syndrome protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIV1_ECTSI)

HSP 1 Score: 311 bits (797), Expect = 2.420e-89
Identity = 252/774 (32.56%), Postives = 354/774 (45.74%), Query Frame = 1

Query:  823 LQSASLREIKVDLKALFAIRYKGSGFKLPQVVYTDMCCEDRAL----FAEIVQALREEGHDLAVDGEISPPPAG--PVLDLPPDVEVACI---KASKETPGVAAAAQNIRQQAEAHGRVVGLDLEWEISRFGAPGNPPATIQLAAGK-QAVIFHVLHG-QRSSPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYGVDVKNVVDLPALALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQKRLRFLSDPIFTDAK-ELNAGNYVRLYTRGGNDCVGEGRIIEYHKETWGTTGIFVSPRRSTRGLTRRWVVQLVTVHVHRAKTLYPD--ECDDRHTQVTAQVGDRKDQRLLWDAGRLRKVSVNTGVTTSESGHAGGRCATAGDRGGINPGLGSPALRPNGEEMVRCVDASGVTPGMLPHAPAAAVPLSTT-GPGGGGVTPSESFFSGDFSGLTRD---FWCSILSRGV----------------------------------------------QEARLCVKLDAFHGMQRLSKLILKNHGACRAFLARLRDAFFKVYAEDIAGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEGLAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSMLFGEAE-YSSWLPTSAYGDTGERNGFFRSV 2949
            + +AS+ ++K DLK +F  R+ G GFKLP V+++D CC+DR L    F EI        +D A +     P     P L+ PP     C+   + +   P V A     + Q    G V+GLD EWE S  G   NP +T+QL+     A  F +  G ++++    P ++   LE+ SIAKVGV I  D T L   YG++V N VDL   A    V+    R LA +++ LLG+ L K+  +R SRW+   LSD+Q+  +F  DPIF+    EL+ G  VRLY+     CVG G + +         G F   RR  +G   + VV L  V    A T +           +VT     + D+ +LWD   +R VS                          +P   +P+   N  E     +A   T    PH     V L     P  G     +    GD     R    FW  + + G                                               ++  L V+LD FH +QR+S+L  K+HGA + F+ARLRDA F V  +DI  VE AL   GM+ E VA  K  +W +FL+ CRR++P     L+R + VI  F  ++D K+ + L+R  A  A  +LR HI + CLSDP  V LY+  G+    G    RCVRGTN           E NYRWN+R AVKNRGL   VG F++Q  +E IQR TS  + +   +  W+      DTGE++G  +S+
Sbjct:    1 MTTASVHDLKNDLKMMFVKRFLGHGFKLP-VIFSDECCDDRQLLLTMFKEIELETGARLYDTAEEAGGGSPGVEVLPALEFPPGKRGECVFDFRPNSIAPSVVALTDGCQLQ----GNVLGLDCEWEPSLAGTTPNPVSTVQLSLPDGTAYCFQLQRGNKKTTSSNFPKALQNLLENPSIAKVGVNINSDATYLERDYGIEVANTVDLRTYARQCWVETP-SRSLAGMASSLLGRQLPKDPVIRSSRWSSP-LSDNQEVAKF-QDPIFSPPPVELHPGTKVRLYSNNHASCVGVGEVQDDDTAAEALAGWFPGARR--KGRLTQVVVGLSGVFKPAAYTAHKGTPSAASGVGRVTMDEAYKGDRLVLWDVAHVRLVSDWK----------------------PSPAAAAPSSNENASEAYND-NAQFFTSPRPPHV----VRLQDDEDPDDGDEEEYDGHDCGDLGSSARGDHGFWAGVEAGGGGGGAVNGTXXXXXXXXXXXXXXXXXXXXXXXXXXXGDVDMDDWLGDEQCALQVRLDIFHALQRISRLCKKSHGAFKPFMARLRDACFIVNQDDIKEVEQALGSKGMTPEAVAEYKDKNWTYFLRNCRRLVPERQRLLERFNSVINQFCNVVDAKSGEVLIRPKAMQAVDQLRKHIESDCLSDPHGVPLYYTTGQN-AAGITTRRCVRGTN---------YTEFNYRWNIRMAVKNRGLPREVGEFFDQFEIEIIQRETSSWYPDNPLFPEWISALDAADTGEKSGLSQSL 727          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: D7G3Q9_ECTSI (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3Q9_ECTSI)

HSP 1 Score: 280 bits (715), Expect = 4.490e-77
Identity = 247/824 (29.98%), Postives = 358/824 (43.45%), Query Frame = 1

Query:  550 FGTFDAPDGYNDFFPSPHYLSTAWTEFMQE------------RSDFMQQRHQHVDGVVMKIDASHKLVKLVRVYAPGAGADTKPTYGVITIFNEFEQVVFQKALQSASLREIKVDLKALFAIRYKGSGFKLPQVVYTDMCCEDRALFAEIVQALREEGHDLAVDGEISPPPAGPVLDLPPDVEVACIKASKETPGVAAAAQNIRQQAEAHGRVVGLDLEWEISRFGAPGNPPATIQLAAGK-QAVIFHVLHGQRS-SPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYGVDVKNVVDLPALALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQKRLRFLS---------------DPIFTDA-KELNAGNYVRLYTRGGNDCVGEGRIIEYHKETWGTTGIFVSPRRSTR-GLTRRWVVQLVTV-----HVHRAKTLYPDECDDRHTQVTAQVGDRKDQRLLWDAGRLRKVSVNTGVTTSESGHAGGRCATAGDRGGINPGLGSPALRPNGEEMVRCVDASGVTPGMLPHAPAAAVPLSTTGPGGGGVTPSESFFSGDFSGLTRDFWCSILSRGVQEARLCVKLDAFHGMQRLSKLILKNHGACRAFLARLRDAFFKVYAEDIAGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEG-----------------LAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSM 2862
            FG F   +G+N  +PS  YL   W ++  +            R D++Q+  Q VDG+V+  DAS K  K++R+ + G    +KP  G+ TI NE+EQ             E   +L AL     K                         V+   +   D+AV                                      N+       G V+G+D EWE +  G+   P  T+QLA     + +FH+  G R  +P    +++   L D +I KVGV +K D  RL   Y V+ + +VDL + A +  VD+  R  LA ++A  LGK L K+   R S W++  L+ +Q     L                DPIF +A  E+ A          G     E   +    E WGT  + +  R  TR G  +R VV++         V   K   P    D   +VT +        +LWD   +R  S +     +     G   A A      +    + A+  NG ++   ++A   +         A   L+++    GG  PS S    D          ++ S+        V+LD FH + RLS+ I K HGA R  +ARLRDA F V  +D+  +E  L + GMS  +V   K  DW++FL++CRR++PP  E L+R D V++ FG  +D  +K+ LLR  +  A   LR HI  GCLSDP  V++Y+  GK    G   YRCVRGTND+EG                 LAQ +LLE N+RWNL+  VKNRGL A +G  Y Q ++E IQR T +
Sbjct:   45 FGGFGDKEGFNGAWPSDQYLRAVWHKWFYDTVVIEADGVRMTREDYLQRVGQLVDGMVLAGDASFKYAKVIRLTSAGESTKSKPIQGIFTILNEYEQAA-----------EGGAELAALTFPEGKTG-----------------------VRVFDKTAIDIAV-------------------------------------ANLVGGCATQGMVLGMDCEWEPAFNGSSERPVCTLQLALPDGTSSLFHLQRGGRGITPSTFNTNLKRLLGDLAITKVGVAVKGDGKRLQRDYDVETRGMVDLRSYARACWVDLPCR-SLAGMTATALGKYLSKDAAGRFSHWSRPELTPNQIEYACLDAYAAVLLHAEIGKFKDPIFGEAPSEVPAA---------GTKTEEEATSL---LEKWGTV-LTLGGRVGTRSGGLQRVVVKVDNALKPSSFVPHDKA--PPPAQDGQRRVTVKDVVDGAGFVLWDIVHVRLASAHRSFEGTPL-PGGAADADAHQDDFFDAPRPAQAVYQNGGDVESDLEADSESDDDTFGESGAGADLASSEASSGG--PSASXXXXD---------AAVASQEEAGDMFFVRLDLFHALNRLSRTIKKGHGAFRPIMARLRDACFLVNRDDVLQIEKVLERRGMSPAQVQQAKDQDWSYFLRKCRRLVPPPSELLKRFDKVVQEFGNCVDNISKEVLLRPKSMKAVSLLRKHIVAGCLSDPDGVAMYYAVGKT-TDGLVNYRCVRGTNDVEGYHRYLRTLLASYCASPALAQSVLLEFNFRWNLKMMVKNRGLPAELGEHYNQHLVEIIQRATEL 768          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A6H5KFL9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KFL9_9PHAE)

HSP 1 Score: 258 bits (659), Expect = 2.680e-75
Identity = 145/270 (53.70%), Postives = 174/270 (64.44%), Query Frame = 1

Query:  406 VHSKGFAASSEALAQAHLKKFHDAELKYYNMALWRRHSAL-GPVATESRFGTFDAPDGYNDFFPSPHYLSTAWTEFMQE--------------------RSDFMQQRHQHVDGVVMKIDASHKLVKLVRVYAPGAGADTKPTYGVITIFNEFEQ-----VVFQKALQSASLREIKVDLKALFAIRYKGSGFKLPQVVYTDMCCEDRALFAEIVQALREEGHDLAVDGEISPPPAGPVLDLPPDVEVA-CIKASKETPGVAAAAQNIRQQA 1134
            +H+KGF+ASSEAL QAHL +FH  ELKYY+M LWRR   L G V  E  FG+F+  DGYN F PS HYLST W E MQ                     R DF+Q+R Q VDG V+K+DAS KL KLVRV APGA ADTKP + V+T+FNEFEQ     VV QKALQS SLRE+K DLKALF  RYKG GFKLP++V TD CC D+AL  +I + L  EGH  +V   +      PVL LPPDV +   +KA++++P + AA    R QA
Sbjct:    1 MHAKGFSASSEALQQAHLYRFHSMELKYYSMLLWRREEQLDGGVQDE--FGSFEDADGYNGFSPSEHYLSTVWCERMQRSRAARLDKHVNGVQGESKWTREDFLQRRQQQVDGTVLKVDASFKLAKLVRVRAPGAAADTKPFHCVVTLFNEFEQATQIVVVLQKALQSGSLRELKQDLKALFVTRYKGGGFKLPRLVSTDECCNDKALLNDIFEELASEGHQFSVTNSVPIASECPVLQLPPDVVLGEPVKANQDSPCLKAAVDTTRMQA 268          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A1Y2A9B5_9FUNG (3'-5' exonuclease domain-containing protein n=1 Tax=Rhizoclosmatium globosum TaxID=329046 RepID=A0A1Y2A9B5_9FUNG)

HSP 1 Score: 270 bits (691), Expect = 1.290e-74
Identity = 223/718 (31.06%), Postives = 326/718 (45.40%), Query Frame = 1

Query:  904 LPQVVYTDMCCEDRALFAEIVQALREEGH--DLAVDGEISPPPAGPVLDLPPDVEVACIKASKETPGVAAAAQNIRQQAEAHGRVVGLDLEWE---ISRFGAPGNPPATIQLA--AGKQAVIFHVLHGQRSSPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYG---VDVKNVVDLPALALSR-------KVDIGLRR----GLAD-----LSAHLLGKILRKEQ--HLRISRWNQAMLSDDQKRLRF-LSDPIFTDAKELNAGNYVRLYTRGGNDCVGEGRIIEYHKETWGTTGIFVSPRRSTRGLTRRWVVQLVTVHVHRAKTLYPDECDDRHTQVTAQVGDRKDQRLLWDAGRLRKVSVNTGVTTSESGHAGGRCATAGDRGGINPGLGSPALRPNGEEMVRCVDASGVTPGMLPHAPAAAVPLSTTGPGGGGVTPSESFFSGDFSGLT----RDFWCSILSRGVQEAR-----LCVKLDAFHGMQRLSKLILKNHGACRAFLARLRDAFFKVYAEDIAGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEG-----------------LAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSMLFGEAEYSSW 2892
            +P ++YTD CC+DR    ++   L++     D+ +        A   L  P + E   I  S +   +      IRQ      +V+G D+E+    I     P   PATIQL+  AG   V    + G++   E +P+++   L D     VG  IK D TRL   Y     D  N +DL   A+SR        +D+  R+    GLA      LS   LGK L K+Q  +  +  +   ++ ++    R   S+PI      L  G  V ++T+   +CV    I+EY +  WG       P +      +R V++L  V  + A ++Y    D    +    + +   Q +LWD   LR       +   ES                        L  + + +++  +   +TP  +      ++P+S     G  V   E+ F+ +          D             R     L V+LD FH M R++K +LK+HGA + F+ARLRDAFF +  +D+  V AAL  AG+S +E+  KK  DWAFFL+ C R+IP   E L+R D V   F  +LD KT +PL R++ +   + LR HI  GCLSD  D+ LYF EG+    G P+YRC RGTN  EG                 L  LLLLE N+RWN+R AVKNRGL +  GGFY Q +L+ I R +S L     Y +W
Sbjct:    1 MPLILYTDRCCDDREFLRQLFNELKQTDSFFDVQLGEREDAAEALEYLQFPANKEPVHILTSDKD-ALQLVCDMIRQTVTQGPQVLGFDIEYNPGFIPAADGPSVAPATIQLSNLAGDSWVFSLFVDGKKK--ESIPNALQTLLTDNRFTFVGASIKGDITRLEKHYSNVMADNINYLDLSESAVSRGYTVQSKSLDMLTRQFCKFGLAKDSRVRLSNWTLGKKLTKQQLHYAALDAYASRLVHENIVAKRDPRSEPI---PDSLQPGTKVFVFTKSDAECVARATIVEYSEPKWG-------PLQLKTASKQRVVIKLDEVLNNSAISMYRQRLD----KDIKPLRELHSQTVLWDIAHLR-CETQESLAWMES------------------------LAISSDRVIQSSEQDFITPSDIFFT---SIPISR----GSVVESDENLFTDENDASDCESEADXXXXXXXXXXXXXRPDGFILKVRLDCFHAMNRINKTLLKSHGAYKPFMARLRDAFFLISKDDLDLVVAALKTAGLSDDEIEAKKDYDWAFFLKYCHRLIPCKEELLKRYDFVCNTFQDILDSKTNEPLFRSNTKKEVRNLRKHIQNGCLSDVPDIPLYFLEGRTS-QGLPIYRCARGTNSNEGYHRHMRKLLHHYASSPMLLHLLLLEFNFRWNIRMAVKNRGLPSEYGGFYSQSLLDEINRNSSGLSVHVPYLAW 668          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A6H5JRJ6_9PHAE (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JRJ6_9PHAE)

HSP 1 Score: 251 bits (641), Expect = 1.200e-65
Identity = 222/683 (32.50%), Postives = 301/683 (44.07%), Query Frame = 1

Query: 1144 GRVVGLDLEWEISRFGAPGNPPATIQLAAGK-QAVIFHVLHG-QRSSPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYGVDVKNVVDLPALALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQKRLRFLS---------------DPIFTDAKE--LNAGNYVRLYTRGGNDCVGEGRIIEYHK-----ETWGTTGIFVSPRRSTR-GLTRRWVVQLVTVHVHRAKTLYPDE-----CDDRHTQVTAQVGDRKDQR-----LLWDAGRLRKVSVN--------TGVTTSESGHAGG-----RCATA--GDRGGINPGLGSPALRPNGEEMVRCVDASGVTPGMLPHAPAAAVPLSTTGPGGGGVTPSESFFSGDFSGLTRDFWCSILSRGVQEARLCVKLDAFHGMQRLSKLILKNHGACRAFLARLRDAFFKVYAEDI-------------AGVEAALLKAGMSQEEVAVKKRTDWAFFLQRCRRIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEG-----------------LAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSML-FGEAEYSSWLPTSAYGDTGERNGFFRSV 2949
            G V+G+D EWE    G+      T+QLA     A +FH+  G +R+ P     S+   L D +I KVGV +K D +RL   YGV+   +VDL + A +  VD+  R  LA + A  LGK L K   +R SRW+QA L+ +Q     L                DPIF  A      AG  VRLYT     CVG G++ +        E WGT  + ++ R  TR G  +R VV++V V    + T + D            +V   + D    +     +LWD   +R  S +           +     H        R A A   + G +   LG     P+G      +D S    G                 G                             GV      V+LD FH + R+S++  K+HGA + F+ARLRDA F V  +D+               VE  L   GM  EEV      +W+FFL+  RR++P     L+R D VI  FGG +D  + + LLR  A  A ++LR HI   CLSDP  +++YF  GK    G   YRCVRGTND+EG                 LAQ +LLE NYRWNLR  +KNRGL A++GG Y Q ++E+IQR T+        +  W       DTGER G  RS+
Sbjct:   87 GHVLGMDCEWEPPFDGSSEGAVCTLQLALPDGTAYLFHLQRGTRRTRPSTFNRSLKVLLNDPTITKVGVAVKGDGSRLQRDYGVETCGMVDLRSYARTCWVDLPCR-SLAGMVATALGKSLSKNPSVRFSRWSQAELTQNQVNYACLDAYAAVVLHAEIGKSKDPIFCMAPSDIPIAGTEVRLYTNNHESCVGVGQVQDEEAARSLVEKWGTD-LKLAQREGTRSGGLKRVVVKVVDVLKPASLTPHQDAPPXXXXXXGQPRVAVSMNDVHSGKGFEGFVLWDLVHVRLASDHHPFQGIPVPAASADPDAHVDNFFDEPRPAQAVYQNDGDVESALGG---EPDGSSDDDTLDESAAGVGAXXX-----XXXXXXXKGAASAAXXXXXXXXXXXXXXXXXXAPEEPHGV----FSVRLDPFHTLNRISRMTKKSHGAFKPFMARLRDACFLVNRDDVYQASLKPFGWIWERSVEMTLEARGMKPEEVQQLTEQNWSFFLRSSRRLVPRRDVLLKRFDRVIEEFGGCVDNTSGEVLLRPKAMKAVRQLRKHIENDCLSDPDGMAMYFAIGKNK-HGLTDYRCVRGTNDVEGYHRYLRTLLASYCASPSLAQSVLLEFNYRWNLRMMIKNRGLPAALGGHYNQYLVESIQRDTADWDSSHPVFPEWPAAIDSVDTGERFGLERSL 754          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A6H5JQG2_9PHAE (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQG2_9PHAE)

HSP 1 Score: 251 bits (642), Expect = 1.410e-65
Identity = 184/559 (32.92%), Postives = 280/559 (50.09%), Query Frame = 1

Query:   67 RSVFVWIPELTHPQSLPDGRPPCPRCKTAHRVIIKGWTQKLSRRAIMRDACCDLLGYFYKCQHCSTRVTG---------TFQSWDAGVLSQLPLFVQECFPFVLTRKSAIHVDVLLELSDNLVHSKGFAASSEALAQAHLK--------------------------KFHDAELKYYNMALWRRHSA-----LGPVATESRFGTFDAPDGYNDFFPSPHYLSTAWTEFMQER------------SDFMQQRHQHVDGVVMKIDASHKLVKLVRVYAPGAGADTKPTYGVITIFNEFEQVVFQKALQSASLREIKVDLKALFAIRYKGSGFKLPQVVYTDMCCEDRALFAEIVQALREE-GHDLAVDGEISPPPAGPVLDLPPDVEVACIKASKETP-GVAAAAQNIRQQAEAH---GRVV-GLDLEWEISRFGAPGNPPATIQLAAGK-QAVIFHVLHGQRS-SPEKLPSSVVEFLEDASIAKVGVGIKQDCTRLSNFYGVDVKNVVDLPALALSRKVDIGLRRGLADLSAHLLGKILRKEQHLRISRWNQAMLSDDQK 1563
            ++V VWIPE   P+       PCP C    +    GW  K  RR  M      ++G+ YKC  C+    G         +F +WDAG L +LP ++ + FP +LT++S I + ++  L+D+LVH KGF+A+++ + Q ++                           KF   +LKY ++   RR S+          T  RFG+FD    Y    PS HYL   W  +  +              ++  +  Q  DG V+  DAS K  K++R+ A      +KP YG+ T+FNE+EQVV+Q+ +++ +L E+  +LK  F  R+  +GFK+  +  TD CC+DR L   + +   E  G  L ++ E+    AG  L+L P +++    A    P  VAAA  ++RQ    +   G++V G D EW+ S  G    P AT Q+AA +  + +FH+  G    + E  P+ +   LED+SI K GV I  D T + N YGV + + VDL  LA+SR V +   R LA L+A L+G+ L K+  +R SRW    LSD+Q+
Sbjct:  100 KAVLVWIPEFLFPELQAV---PCPGC--GGKGAPDGWNPKGPRRVFMEHDVAYIMGFRYKCAKCAKFNQGKEEAEKRKTSFNAWDAGCLQRLPEYISKEFPSLLTKRSGIEIRMVDRLADDLVHGKGFSAAAKNICQVNVHFLICSVLKITXXXXXXXXXXXXXXTTKFMVDQLKYTSLVNSRRTSSGIFRSANHAKTPERFGSFDDSTKYGGAVPSEHYLRDVWWLYYSKLPVLEVDGVGWTLEEYQHRLMQRWDGEVLSGDASFKFAKIIRLGARAGEERSKPVYGLFTVFNEYEQVVWQRPMKTGALSELVEELKPFFN-RFVRNGFKVFTLWNTDDCCDDRQLLQRVFREFEEATGISLHLEDEVD---AGVQLELLPPLDLEFTPAIVYDPEAVAAACGSLRQIISLNRVEGKMVCGADAEWQFSPRGR--EPVATFQIAALRGPSFLFHLQRGSSGFTKETFPAPLKALLEDSSIIKAGVAINTDATHMLNDYGVKMASTVDLRVLAVSRLV-VTSSRTLAGLTACLVGRQLPKDT-VRCSRWGSPRLSDEQR 645          

BLAST of mRNA_M-pyrifera_M_contig11885.3873.1 vs. uniprot
Match: A0A6H5JD89_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JD89_9PHAE)

HSP 1 Score: 226 bits (577), Expect = 6.550e-61
Identity = 119/201 (59.20%), Postives = 147/201 (73.13%), Query Frame = 1

Query: 2464 RIIPPAPEALQRSDLVIRVFGGLLDKKTKQPLLRADARLAAKRLRSHIATGCLSDPKDVSLYFQEGKEYVTGFPLYRCVRGTNDLEG----------------LAQLLLLEHNYRWNLRQAVKNRGLDASVGGFYEQPVLEAIQRMTSMLFGEAEYSSWLPTSAYGDTGERNGFFRSVLPEGS-GGGTDFLDDLTIGGNES 3015
            R+IP   E L+R DLV  ++GGL+DKKTKQPL RA+A  A KRLR HI   C SD  + +LYF+ GK+  T  PL+RC+RGTNDLEG                LA LLLLE+NYRWNLRQ++KNRGLD +VGGFY+QP+LEAIQRMTS L+G+A+YS WLPTSAYGDTGER+GF RS+ P    GGGTD + ++ +GG E+
Sbjct:  206 RLIPRPAELLERFDLVNNLYGGLIDKKTKQPLFRAEALEAIKRLRVHIMNDCCSDSPNEALYFEAGKDAETLCPLFRCIRGTNDLEGYHLHMRLLVAWCISPRLAHLLLLEYNYRWNLRQSMKNRGLDEAVGGFYDQPMLEAIQRMTSKLYGKAKYSQWLPTSAYGDTGERSGFVRSIFPSNVVGGGTDSVLEI-LGGAEN 405