mRNA_M-pyrifera_M_contig117082.3527.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig117082.3527.1
Unique NamemRNA_M-pyrifera_M_contig117082.3527.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: A0A8J4PMF2_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PMF2_9MYCE)

HSP 1 Score: 129 bits (323), Expect = 5.540e-32
Identity = 63/133 (47.37%), Postives = 88/133 (66.17%), Query Frame = 1 
Query:    4 GDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            GD+R+  KYTEE+V PL  Y  LL LL D++L  IH +G VT  +E V   L  +FE   K + L++ +T+ EID+T +PD+IFR N+L TK++D +MKL G+PYL QT+  L     + KK C + PT+LEK
Sbjct:  506 GDIRLKLKYTEEVVLPLQSYMPLLDLLQDDNLYTIHMMGRVTKQREAVSSNLIRVFEKTNKCIFLLKALTDHEIDSTDNPDIIFRGNSLATKSVDLYMKLVGIPYLSQTIGPLIKKIYSSKKSCEIDPTKLEK 638
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: NGAP_DICDI (Probable Ras GTPase-activating-like protein ngap n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=NGAP_DICDI)

HSP 1 Score: 129 bits (323), Expect = 5.580e-32
Identity = 64/133 (48.12%), Postives = 88/133 (66.17%), Query Frame = 1 
Query:    4 GDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            GD+R+  KY+EE+V PL  Y  LL LL  E+L  I  LG VT  +E V   L  +FE  GK + L++ +T+ EID+T++PD+IFR N+L TK++D FMKL G+PYL QT+  L     + KK C + PT+LEK
Sbjct:  532 GDIRLKLKYSEEVVLPLQSYQPLLELLQQENLYTITLLGKVTKHRESVSNNLIRVFEKTGKCLYLLKSLTDHEIDSTNNPDIIFRGNSLATKSVDLFMKLIGIPYLSQTIGPLIKKIYSSKKSCEIDPTKLEK 664
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: F4PLF8_CAVFA (RasGTPase-activating protein n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4PLF8_CAVFA)

HSP 1 Score: 128 bits (322), Expect = 7.250e-32
Identity = 61/133 (45.86%), Postives = 84/133 (63.16%), Query Frame = 1 
Query:    4 GDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            GDVR+  KYTEE+V PL  Y  LL+ L  ED+  +H LG++T  +E V   L  +FE  G  + L++ +T  EID T +PD+IFR N+L TK++D +MKL G+PYL  T+  L       KK C + PT+LEK
Sbjct:  422 GDVRLKLKYTEEVVLPLESYQPLLSHLQQEDMSAVHILGNITKQREAVANNLIRVFEKTGHCLSLLKSLTEHEIDTTDNPDIIFRGNSLATKSVDLYMKLIGLPYLANTIGPLIKKIYVSKKSCEIDPTKLEK 554
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: F1A5K7_DICPU (Uncharacterized protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F1A5K7_DICPU)

HSP 1 Score: 128 bits (321), Expect = 1.040e-31
Identity = 63/133 (47.37%), Postives = 88/133 (66.17%), Query Frame = 1 
Query:    4 GDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            GD+R+  KY+EE+V PL  Y  LL LL +E L  IH LG VT  +E V   L  +FE  G  + L++ +T+ EID+T++PD+IFR N+L TK++D +MKL G+PYL QT+  L     + KK C + PT+LEK
Sbjct:  520 GDIRLKLKYSEEVVLPLQSYSPLLNLLQEEHLYTIHLLGKVTKHREAVSNNLIRVFEKTGNCLYLLKSLTDHEIDSTNNPDIIFRGNSLATKSVDLYMKLIGIPYLAQTIGPLIKKIYSSKKSCEIDPTKLEK 652
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: UPI000644DA2A (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644DA2A)

HSP 1 Score: 124 bits (312), Expect = 1.670e-30
Identity = 58/134 (43.28%), Postives = 88/134 (65.67%), Query Frame = 1 
Query:    1 LGDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            LGDVR+  KY+EE+V PL  Y  LL  + D+++ VI+ LG+++  +E +   L  +FE   +  +L+R +T +EID+T +PD+IFR N+L TK++D +MKL G+PYL  T+  L       KK C + PT+LEK
Sbjct:  455 LGDVRLKLKYSEEVVLPLKSYGPLLEFIQDDEMSVINILGNISKQRETIANILIRVFEKTDRCADLLRRLTEQEIDSTDNPDIIFRGNSLATKSVDLYMKLIGLPYLASTIGPLIKKIYVSKKSCEIDPTKLEK 588
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: A0A152A7V2_9MYCE (RasGTPase-activating protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A152A7V2_9MYCE)

HSP 1 Score: 118 bits (296), Expect = 2.430e-28
Identity = 60/133 (45.11%), Postives = 85/133 (63.91%), Query Frame = 1 
Query:    4 GDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            GDVR+  KY+EE+V PL  Y   L  L D++L  IH LG VT  +E V   L  +FE  GK V L++ +T+ EI++T +PD+IFR N+L TK++D +MKL G+PYL  T+        + KK C + PT++EK
Sbjct:  539 GDVRLKLKYSEEVVLPLESYLNFLNHLQDDNLYSIHLLGHVTKQREAVSHNLIRVFEKTGKCVFLLKSLTDHEIESTDNPDIIFRGNSLATKSVDLYMKLIGLPYLTATIGPHIKKIYSSKKSCEIDPTKIEK 671
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: UPI000644D753 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644D753)

HSP 1 Score: 111 bits (277), Expect = 8.620e-26
Identity = 56/134 (41.79%), Postives = 83/134 (61.94%), Query Frame = 1 
Query:    1 LGDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            LGDVR+  KY+EE+V PL  Y  LL LL +E L ++H L + ++ +E +   L  +FE   + V LI  +T++EI+ T +PD+IFR N+L TK+LD +MKL G+ YL  T+  L        K C + PT++ K
Sbjct:  404 LGDVRLKLKYSEEVVLPLQSYMPLLRLLQNERLTLVHILSNTSSNREQLASILIRVFEKSDRCVSLITSLTDKEIEITDNPDIIFRGNSLATKSLDLYMKLIGLKYLAGTVGPLVKKIVKANKSCEIDPTKMAK 537
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: A0A0D2VRU1_CAPO3 (Uncharacterized protein n=1 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2VRU1_CAPO3)

HSP 1 Score: 100 bits (250), Expect = 3.660e-22
Identity = 58/135 (42.96%), Postives = 83/135 (61.48%), Query Frame = 1 
Query:    1 LGDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNT---SDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRL 396
            LG ++  +KYTEE + PL EY+ LL LL+DE L VI ALG + T +EDV RTL  IF+ K    + +R M   +I +T   +D +++FR N++GTKT++ +MKL G  YL   L+ L        + C V PT+L
Sbjct:  253 LGLLKFKYKYTEEFILPLQEYEELLDLLLDEQLAVIIALGKLATDREDVARTLLRIFDGKTVTPKYVRLMYMLDIKHTVAANDVNLLFRGNSIGTKTVEMYMKLVGQQYLTAVLKPLIKGIYDCPQSCEVDPTKL 387
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: A0A2P6N4L5_9EUKA (Uncharacterized protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6N4L5_9EUKA)

HSP 1 Score: 100 bits (248), Expect = 6.980e-22
Identity = 50/134 (37.31%), Postives = 85/134 (63.43%), Query Frame = 1 
Query:    1 LGDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK 402
            LGD+R+  KY+E  +  L  Y  L  L+   D  +I ALG+VT+ ++ V + L  ++E +GKAV+L+  +T REI +T+  DVIFR N++ TK++D +MKL G+ +L + + +   +  + +K   V P+R++K
Sbjct:  400 LGDIRLRLKYSETQILTLDNYSELWDLINAGDYAIIDALGEVTSDRDGVAKNLVRLYEGRGKAVDLLNHLTEREISSTASEDVIFRGNSIATKSVDNYMKLIGLKFLYKVMARKVKEICSSRKSFEVDPSRVDK 533
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BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Match: A0A2P6N0S1_9EUKA (RasGTPase-activating protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6N0S1_9EUKA)

HSP 1 Score: 99.8 bits (247), Expect = 9.490e-22
Identity = 46/110 (41.82%), Postives = 76/110 (69.09%), Query Frame = 1 
Query:    4 GDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVGRTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMKLAGMPYLKQTL 333
            GD+R+  KYTE  +  L +Y+ L  ++  +D  V+ ALG+VT  ++D  + L  +F++K KA+ ++  +T RE+++T   DVIFR N++ TK+LDY+MKL G P+LK+T+
Sbjct:  390 GDIRLRMKYTELKILKLDQYEELWKVVDTQDYAVVDALGEVTGERDDCAKNLVRLFQAKSKAIPMLIHLTTREVNDTPSADVIFRGNSIATKSLDYYMKLMGGPFLKRTI 499
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig117082.3527.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8J4PMF2_9MYCE5.540e-3247.37Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
NGAP_DICDI5.580e-3248.12Probable Ras GTPase-activating-like protein ngap n... [more]
F4PLF8_CAVFA7.250e-3245.86RasGTPase-activating protein n=1 Tax=Cavenderia fa... [more]
F1A5K7_DICPU1.040e-3147.37Uncharacterized protein n=1 Tax=Dictyostelium purp... [more]
UPI000644DA2A1.670e-3043.28hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A152A7V2_9MYCE2.430e-2845.11RasGTPase-activating protein n=1 Tax=Tieghemosteli... [more]
UPI000644D7538.620e-2641.79hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A0D2VRU1_CAPO33.660e-2242.96Uncharacterized protein n=1 Tax=Capsaspora owczarz... [more]
A0A2P6N4L5_9EUKA6.980e-2237.31Uncharacterized protein n=1 Tax=Planoprotostelium ... [more]
A0A2P6N0S1_9EUKA9.490e-2241.82RasGTPase-activating protein n=1 Tax=Planoprotoste... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig117082contigM-pyrifera_M_contig117082:152..553 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score131.0
Seed ortholog evalue4.3e-28
Seed eggNOG ortholog44689.DDB0220496
KEGG rclassRC00299
KEGG koko:K01613,ko:K05039,ko:K05841,ko:K12380
KEGG TC2.A.22.3
KEGG ReactionR02055
KEGG Pathwayko00564,ko01100,ko01110,ko04013,ko04014,map00564,map01100,map01110,map04013,map04014
KEGG ModuleM00093
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005096,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005813,GO:0005815,GO:0005829,GO:0005856,GO:0005886,GO:0006629,GO:0008047,GO:0008150,GO:0008152,GO:0008194,GO:0008202,GO:0009898,GO:0009966,GO:0009968,GO:0010646,GO:0010648,GO:0015020,GO:0015630,GO:0016020,GO:0016125,GO:0016740,GO:0016757,GO:0016758,GO:0016906,GO:0023051,GO:0023057,GO:0023058,GO:0030234,GO:0030695,GO:0031224,GO:0031226,GO:0031235,GO:0031252,GO:0031256,GO:0035251,GO:0043085,GO:0043087,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043547,GO:0044093,GO:0044238,GO:0044422,GO:0044424,GO:0044425,GO:0044430,GO:0044444,GO:0044446,GO:0044459,GO:0044464,GO:0046527,GO:0046578,GO:0046580,GO:0048519,GO:0048523,GO:0048583,GO:0048585,GO:0050789,GO:0050790,GO:0050794,GO:0051056,GO:0051058,GO:0051336,GO:0051345,GO:0051505,GO:0051506,GO:0051507,GO:0051508,GO:0051509,GO:0060589,GO:0065007,GO:0065009,GO:0071704,GO:0071944,GO:0098552,GO:0098562,GO:0098772,GO:1901360,GO:1901615,GO:1902531,GO:1902532
EggNOG free text desc.GTPase-activator protein for Ras-like GTPases
EggNOG OGs3XCYB@554915,KOG1032@1,KOG1032@2759,KOG2059@1,KOG2059@2759
EC2.4.1.173,4.1.1.65
COG Functional cat.T
CAZyGT1
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003,ko02000
Exons1
Model size402
Cds size402
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815687.7235057-CDS-M-pyrifera_M_contig117082:151..5531622815687.7235057-CDS-M-pyrifera_M_contig117082:151..553Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig117082 152..553 +
1692277522.285404-CDS-M-pyrifera_M_contig117082:151..5531692277522.285404-CDS-M-pyrifera_M_contig117082:151..553Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig117082 152..553 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig117082.3527.1prot_M-pyrifera_M_contig117082.3527.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig117082 152..553 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig117082.3527.1

>prot_M-pyrifera_M_contig117082.3527.1 ID=prot_M-pyrifera_M_contig117082.3527.1|Name=mRNA_M-pyrifera_M_contig117082.3527.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=134bp
LGDVRIVFKYTEEIVCPLVEYDALLALLMDEDLDVIHALGDVTTAKEDVG
RTLSHIFESKGKAVELIRGMTNREIDNTSDPDVIFRANTLGTKTLDYFMK
LAGMPYLKQTLQKLTTDASTQKKPCAVAPTRLEK
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mRNA from alignment at M-pyrifera_M_contig117082:152..553+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig117082.3527.1 ID=mRNA_M-pyrifera_M_contig117082.3527.1|Name=mRNA_M-pyrifera_M_contig117082.3527.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=402bp|location=Sequence derived from alignment at M-pyrifera_M_contig117082:152..553+ (Macrocystis pyrifera P11B4 male)
CTGGGCGACGTACGCATCGTATTCAAGTACACGGAGGAGATCGTCTGCCC
GCTGGTGGAGTACGACGCGCTGCTGGCCCTCCTCATGGACGAGGACCTGG
ACGTCATCCACGCGCTGGGCGACGTGACGACCGCCAAGGAGGACGTGGGC
CGTACGCTCTCGCACATCTTCGAGAGCAAGGGCAAGGCCGTGGAGCTGAT
CAGGGGCATGACCAACCGCGAGATCGACAACACCAGCGACCCTGACGTCA
TCTTCCGCGCCAACACGCTCGGCACCAAGACGCTGGACTACTTCATGAAG
CTGGCCGGCATGCCCTACCTGAAGCAGACGCTGCAGAAGCTCACCACCGA
CGCCTCCACCCAGAAGAAGCCCTGTGCGGTCGCCCCCACCCGCCTCGAGA
AG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig117082:152..553+

>mRNA_M-pyrifera_M_contig117082.3527.1 ID=mRNA_M-pyrifera_M_contig117082.3527.1|Name=mRNA_M-pyrifera_M_contig117082.3527.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=804bp|location=Sequence derived from alignment at M-pyrifera_M_contig117082:152..553+ (Macrocystis pyrifera P11B4 male)
CTGGGCGACGTACGCATCGTATTCAAGTACACGGAGGAGATCGTCTGCCC
GCTGGTGGAGTACGACGCGCTGCTGGCCCTCCTCATGGACGAGGACCTGG
ACGTCATCCACGCGCTGGGCGACGTGACGACCGCCAAGGAGGACGTGGGC
CGTACGCTCTCGCACATCTTCGAGAGCAAGGGCAAGGCCGTGGAGCTGAT
CAGGGGCATGACCAACCGCGAGATCGACAACACCAGCGACCCTGACGTCA
TCTTCCGCGCCAACACGCTCGGCACCAAGACGCTGGACTACTTCATGAAG
CTGGCCGGCATGCCCTACCTGAAGCAGACGCTGCAGAAGCTCACCACCGA
CGCCTCCACCCAGAAGAAGCCCTGTGCGGTCGCCCCCACCCGCCTCGAGA
AGCTGGGCGACGTACGCATCGTATTCAAGTACACGGAGGAGATCGTCTGC
CCGCTGGTGGAGTACGACGCGCTGCTGGCCCTCCTCATGGACGAGGACCT
GGACGTCATCCACGCGCTGGGCGACGTGACGACCGCCAAGGAGGACGTGG
GCCGTACGCTCTCGCACATCTTCGAGAGCAAGGGCAAGGCCGTGGAGCTG
ATCAGGGGCATGACCAACCGCGAGATCGACAACACCAGCGACCCTGACGT
CATCTTCCGCGCCAACACGCTCGGCACCAAGACGCTGGACTACTTCATGA
AGCTGGCCGGCATGCCCTACCTGAAGCAGACGCTGCAGAAGCTCACCACC
GACGCCTCCACCCAGAAGAAGCCCTGTGCGGTCGCCCCCACCCGCCTCGA
GAAG
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