mRNA_M-pyrifera_M_contig116177.3357.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig116177.3357.1
Unique NamemRNA_M-pyrifera_M_contig116177.3357.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: A0A067CQQ4_SAPPC (Dolichyl-diphosphooligosaccharide--protein glycotransferase n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067CQQ4_SAPPC)

HSP 1 Score: 181 bits (459), Expect = 8.100e-55
Identity = 82/133 (61.65%), Postives = 108/133 (81.20%), Query Frame = 1 
Query:    1 FDAMRERAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            +D   + + LA V K+ +L  I +++FS+RLF+V+R+ESVIHEFDPYFNFRTTK+L  EGF  F +WFDDRAWYPLGRI+GGTIYPGLM TA+ +YW+LN +N++ N+RN CVFLAP  + NTA+A Y+LT+E
Sbjct:   48 YDIEDDSSTLAPVLKYGLLAAICLMSFSIRLFSVVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAWYPLGRIIGGTIYPGLMYTAALVYWVLNWLNISINVRNTCVFLAPMFAANTAIASYMLTKE 180
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: A0A7S2XUN5_9STRA (Dolichyl-diphosphooligosaccharide--protein glycotransferase (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XUN5_9STRA)

HSP 1 Score: 179 bits (453), Expect = 3.640e-54
Identity = 84/128 (65.62%), Postives = 106/128 (82.81%), Query Frame = 1 
Query:   16 ERAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            E + L+ V + A+L LI  +AFS+RLFAV+R+ESVIHEFDPYFNFRTTK+L  EGF +F +WFDDR WYPLGR +GGTIYPGLMLTA+ ++W LN +N+T NIRN+CVFLAP  + NTA+A +LLT+E
Sbjct:   46 ELSGLSVVFRAAILFLICALAFSIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFLDFLDWFDDRGWYPLGRTIGGTIYPGLMLTAATIHWALNAVNITINIRNMCVFLAPLFAANTALASFLLTKE 173
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: W2HBT5_PHYPR (Dolichyl-diphosphooligosaccharide--protein glycotransferase n=9 Tax=Peronosporaceae TaxID=4777 RepID=W2HBT5_PHYPR)

HSP 1 Score: 190 bits (483), Expect = 8.360e-54
Identity = 87/132 (65.91%), Postives = 112/132 (84.85%), Query Frame = 1 
Query:    4 DAMRERAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            DA  +  ALA V+K+A+L +I +++FS+RLFAV+R+ESVIHEFDPYFNFRTTK+L  EGF  F +WFDDRAWYPLGRI+GGTIYPGLM TA+ +YW+LN++N++ N+RN CVFLAP  + NTA+A YLLT+E
Sbjct:   50 DAEDDENALAPVAKYAILSMICLMSFSIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAWYPLGRIIGGTIYPGLMYTAALVYWVLNLLNISINVRNTCVFLAPLFAANTAIASYLLTKE 181
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: D7G3U2_ECTSI (Dolichyl-diphosphooligosaccharide--protein glycotransferase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3U2_ECTSI)

HSP 1 Score: 182 bits (461), Expect = 1.440e-53
Identity = 85/127 (66.93%), Postives = 103/127 (81.10%), Query Frame = 1 
Query:   19 RAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            R    +V K+A+L +I  +AF +RLFAV+R+ESVIHEFDPYFNFRTTKFL  EGF +F NWFDDR WYPLGR +GGTIYPGLM TA++ YW+L+ +++T NIRNVCVFLAP  + NTAVA YLLT E
Sbjct:   66 RTGFPAVMKYAILAMICALAFFIRLFAVVRYESVIHEFDPYFNFRTTKFLATEGFLDFLNWFDDRGWYPLGRTIGGTIYPGLMATAASFYWILHALHITINIRNVCVFLAPLFAANTAVASYLLTTE 192
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: A0A1E1WUJ9_PECGO (Dolichyl-diphosphooligosaccharide--protein glycotransferase (Fragment) n=1 Tax=Pectinophora gossypiella TaxID=13191 RepID=A0A1E1WUJ9_PECGO)

HSP 1 Score: 176 bits (446), Expect = 2.440e-53
Identity = 79/119 (66.39%), Postives = 101/119 (84.87%), Query Frame = 1 
Query:   43 KFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            K +VL +  +++F+ RLF+V+RFESVIHEFDPYFN+RTT++LT EGFYNFHNWFDDRAWYPLGRI+GGTIYPGLM+T++ LY ++   N+T +IRNVCVFLAP+ S  T +  YLLT+E
Sbjct:   24 KLSVLSMAAILSFATRLFSVLRFESVIHEFDPYFNYRTTRYLTEEGFYNFHNWFDDRAWYPLGRIIGGTIYPGLMVTSATLYNVMQFFNITIDIRNVCVFLAPFFSSLTTIVTYLLTKE 142
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: A0A3M6VAL3_9STRA (Dolichyl-diphosphooligosaccharide--protein glycotransferase n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VAL3_9STRA)

HSP 1 Score: 188 bits (478), Expect = 4.130e-53
Identity = 86/125 (68.80%), Postives = 108/125 (86.40%), Query Frame = 1 
Query:   25 ALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            AL  V+KFA+L +I +++FS+RLFAV+R+ESVIHEFDPYFNFRTTK+L  EGF  F +WFDDRAWYPLGRI+GGTIYPGLM TA+ +YW+LNV+N++ N+RN CVFLAP  + NTA+A YLLT+E
Sbjct:   57 ALTPVAKFAILAMICLMSFSIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAWYPLGRIIGGTIYPGLMYTAALMYWVLNVLNISINVRNTCVFLAPLFAANTAIATYLLTKE 181
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: A0A7S4MGM6_9EUKA (Dolichyl-diphosphooligosaccharide--protein glycotransferase (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4MGM6_9EUKA)

HSP 1 Score: 177 bits (450), Expect = 4.350e-53
Identity = 78/116 (67.24%), Postives = 101/116 (87.07%), Query Frame = 1 
Query:   52 VLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            +L LIYV+AF +RLF+V+R+ESVIHEFDPYFN+R+T++L +EGFYNF NWFD  +WYPLGRIVGGTIYPGLM+TA+++YW+L+  + T +IRNVCV LAPWM+ NTA+  YL T+E
Sbjct:   19 ILSLIYVIAFFVRLFSVLRYESVIHEFDPYFNYRSTQYLVNEGFYNFLNWFDSSSWYPLGRIVGGTIYPGLMVTAASIYWILHTFHFTIDIRNVCVLLAPWMASNTAMVTYLFTKE 134
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: A0A662Y716_9STRA (Dolichyl-diphosphooligosaccharide--protein glycotransferase (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662Y716_9STRA)

HSP 1 Score: 176 bits (446), Expect = 5.610e-53
Identity = 83/141 (58.87%), Postives = 109/141 (77.30%), Query Frame = 1 
Query:   28 LASVSKFAVLGLIYVVAFSMRLFAVIRFESV-----------------IHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            LA ++K+A+LG+I +++F++RLFAV+R+ESV                 IHEFDPYFN+RTTK+L  EGF  F +WFDDRAWYPLGRI+GGTIYPGLM TA+ +YW+LN++N++ N+RN CVFLAP  + NTAVA YLLT+E
Sbjct:   61 LAPIAKYAILGMICLMSFAIRLFAVVRYESVRLGLGKLVIVLNADSRVIHEFDPYFNYRTTKYLASEGFLEFLDWFDDRAWYPLGRIIGGTIYPGLMYTAALVYWVLNLLNISINVRNTCVFLAPLFAANTAVASYLLTKE 201
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BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: K3WH73_GLOUD (Dolichyl-diphosphooligosaccharide--protein glycotransferase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WH73_GLOUD)

HSP 1 Score: 188 bits (477), Expect = 5.960e-53
Identity = 85/132 (64.39%), Postives = 111/132 (84.09%), Query Frame = 1 
Query:    4 DAMRERAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            D   E   LA ++K+A+LG+I +++F++RLFAV+R+ESVIHEFDPYFNFRTTK+L  EGF  F +WFDDRAWYPLGRI+GGTIYPGLM TA+ +YW+LN++N++ N+RN CVFLAP  + NTA+A YLLT+E
Sbjct:   58 DIEDEANVLAPIAKYAILGMICLMSFAIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAWYPLGRIIGGTIYPGLMYTAAMVYWVLNMLNISINVRNTCVFLAPIFAANTAIASYLLTKE 189
[Close]
BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Match: D0MRA6_PHYIT (Dolichyl-diphosphooligosaccharide--protein glycotransferase n=16 Tax=Peronosporaceae TaxID=4777 RepID=D0MRA6_PHYIT)

HSP 1 Score: 187 bits (476), Expect = 6.500e-53
Identity = 85/132 (64.39%), Postives = 111/132 (84.09%), Query Frame = 1 
Query:    4 DAMRERAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTTKFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMNVTANIRNVCVFLAPWMSGNTAVAGYLLTRE 399
            D   +  A+A V+K+A+L +I +++FS+RLFAV+R+ESVIHEFDPYFNFRTTK+L  EGF  F +WFDDRAWYPLGRI+GGTIYPGLM TA+ +YW+LN++N++ N+RN CVFLAP  + NTA+A YLLT+E
Sbjct:   50 DVEDDENAVAPVAKYAILAMICLMSFSIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAWYPLGRIIGGTIYPGLMYTAALVYWVLNLLNISINVRNTCVFLAPLFAANTAIASYLLTKE 181
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig116177.3357.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A067CQQ4_SAPPC8.100e-5561.65Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
A0A7S2XUN5_9STRA3.640e-5465.63Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
W2HBT5_PHYPR8.360e-5465.91Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
D7G3U2_ECTSI1.440e-5366.93Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
A0A1E1WUJ9_PECGO2.440e-5366.39Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
A0A3M6VAL3_9STRA4.130e-5368.80Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
A0A7S4MGM6_9EUKA4.350e-5367.24Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
A0A662Y716_9STRA5.610e-5358.87Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
K3WH73_GLOUD5.960e-5364.39Dolichyl-diphosphooligosaccharide--protein glycotr... [more]
D0MRA6_PHYIT6.500e-5364.39Dolichyl-diphosphooligosaccharide--protein glycotr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig116177contigM-pyrifera_M_contig116177:111..509 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score191.4
Seed ortholog evalue2.6e-46
Seed eggNOG ortholog164328.Phyra72878
Preferred nameSTT3A
KEGG rclassRC00005,RC00482
KEGG koko:K07151
KEGG ReactionR04216,R05976
KEGG Pathwayko00510,ko00513,ko01100,ko04141,map00510,map00513,map01100,map04141
KEGG ModuleM00072
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0003824,GO:0004576,GO:0004579,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0005886,GO:0006464,GO:0006486,GO:0006487,GO:0006807,GO:0006950,GO:0006970,GO:0008150,GO:0008152,GO:0008250,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009628,GO:0009651,GO:0009987,GO:0012505,GO:0016020,GO:0016740,GO:0016757,GO:0016758,GO:0018193,GO:0018196,GO:0018279,GO:0019538,GO:0031984,GO:0032991,GO:0034645,GO:0036211,GO:0042175,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043413,GO:0043687,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0047484,GO:0048583,GO:0050789,GO:0050896,GO:0065007,GO:0070085,GO:0071704,GO:0071944,GO:0080134,GO:0098796,GO:0098827,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1902494,GO:1990234
EggNOG free text desc.Oligosaccharyl transferase STT3 subunit
EggNOG OGs3Q9UB@4776,COG1287@1,KOG2292@2759
EC2.4.99.18
COG Functional cat.O
CAZyGT66
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003
Exons1
Model size399
Cds size390
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815678.629688-UTR-M-pyrifera_M_contig116177:110..1191622815678.629688-UTR-M-pyrifera_M_contig116177:110..119Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig116177 111..119 +
1692277517.9493012-UTR-M-pyrifera_M_contig116177:110..1191692277517.9493012-UTR-M-pyrifera_M_contig116177:110..119Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig116177 111..119 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815678.6393797-CDS-M-pyrifera_M_contig116177:119..5091622815678.6393797-CDS-M-pyrifera_M_contig116177:119..509Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig116177 120..509 +
1692277517.9610193-CDS-M-pyrifera_M_contig116177:119..5091692277517.9610193-CDS-M-pyrifera_M_contig116177:119..509Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig116177 120..509 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig116177.3357.1prot_M-pyrifera_M_contig116177.3357.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig116177 120..509 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig116177.3357.1

>prot_M-pyrifera_M_contig116177.3357.1 ID=prot_M-pyrifera_M_contig116177.3357.1|Name=mRNA_M-pyrifera_M_contig116177.3357.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=130bp
MRERAALASVSKFAVLGLIYVVAFSMRLFAVIRFESVIHEFDPYFNFRTT
KFLTHEGFYNFHNWFDDRAWYPLGRIVGGTIYPGLMLTASALYWLLNVMN
VTANIRNVCVFLAPWMSGNTAVAGYLLTRE
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mRNA from alignment at M-pyrifera_M_contig116177:111..509+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig116177.3357.1 ID=mRNA_M-pyrifera_M_contig116177.3357.1|Name=mRNA_M-pyrifera_M_contig116177.3357.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=399bp|location=Sequence derived from alignment at M-pyrifera_M_contig116177:111..509+ (Macrocystis pyrifera P11B4 male)
TTTGATGCGATGCGGGAGCGGGCGGCGCTGGCGAGCGTGTCGAAGTTTGC
GGTGCTGGGGCTGATCTACGTGGTGGCGTTCAGCATGCGGCTGTTCGCGG
TGATCCGGTTCGAGAGCGTGATCCACGAGTTTGACCCGTACTTCAACTTC
CGCACCACCAAGTTCCTGACGCACGAGGGCTTCTACAACTTCCACAACTG
GTTCGACGACCGCGCGTGGTACCCGCTGGGCCGCATCGTCGGCGGCACCA
TCTACCCGGGCCTGATGCTGACCGCGTCTGCGCTGTACTGGCTGCTGAAC
GTGATGAACGTGACGGCCAACATCCGCAACGTGTGCGTGTTCCTGGCGCC
GTGGATGAGCGGCAACACGGCGGTGGCCGGCTACCTGCTGACGCGCGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig116177:111..509+

>mRNA_M-pyrifera_M_contig116177.3357.1 ID=mRNA_M-pyrifera_M_contig116177.3357.1|Name=mRNA_M-pyrifera_M_contig116177.3357.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=780bp|location=Sequence derived from alignment at M-pyrifera_M_contig116177:111..509+ (Macrocystis pyrifera P11B4 male)
ATGCGGGAGCGGGCGGCGCTGGCGAGCGTGTCGAAGTTTGCGGTGCTGGG
GCTGATCTACGTGGTGGCGTTCAGCATGCGGCTGTTCGCGGTGATCCGGT
TCGAGAGCGTGATCCACGAGTTTGACCCGTACTTCAACTTCCGCACCACC
AAGTTCCTGACGCACGAGGGCTTCTACAACTTCCACAACTGGTTCGACGA
CCGCGCGTGGTACCCGCTGGGCCGCATCGTCGGCGGCACCATCTACCCGG
GCCTGATGCTGACCGCGTCTGCGCTGTACTGGCTGCTGAACGTGATGAAC
GTGACGGCCAACATCCGCAACGTGTGCGTGTTCCTGGCGCCGTGGATGAG
CGGCAACACGGCGGTGGCCGGCTACCTGCTGACGCGCGAGATGCGGGAGC
GGGCGGCGCTGGCGAGCGTGTCGAAGTTTGCGGTGCTGGGGCTGATCTAC
GTGGTGGCGTTCAGCATGCGGCTGTTCGCGGTGATCCGGTTCGAGAGCGT
GATCCACGAGTTTGACCCGTACTTCAACTTCCGCACCACCAAGTTCCTGA
CGCACGAGGGCTTCTACAACTTCCACAACTGGTTCGACGACCGCGCGTGG
TACCCGCTGGGCCGCATCGTCGGCGGCACCATCTACCCGGGCCTGATGCT
GACCGCGTCTGCGCTGTACTGGCTGCTGAACGTGATGAACGTGACGGCCA
ACATCCGCAACGTGTGCGTGTTCCTGGCGCCGTGGATGAGCGGCAACACG
GCGGTGGCCGGCTACCTGCTGACGCGCGAG
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