mRNA_M-pyrifera_M_contig115809.3275.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig115809.3275.1
Unique NamemRNA_M-pyrifera_M_contig115809.3275.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A7S1L9U1_NEODS (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Neobodo designis TaxID=312471 RepID=A0A7S1L9U1_NEODS)

HSP 1 Score: 233 bits (593), Expect = 1.760e-74
Identity = 102/136 (75.00%), Postives = 116/136 (85.29%), Query Frame = 1 
Query:   13 PDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            P++L FP  E  IL  W +IDAF+TS+K SEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIV R+AHQTGH+V RRFGWDCHGLP+E+EI+K LGIKS+ DV+ MGIAAYNAECR IV RY  EWE++V R GRW
Sbjct:   68 PEKLDFPGMEEKILKYWKDIDAFKTSVKKSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVCRFAHQTGHHVERRFGWDCHGLPIEFEIEKALGIKSSHDVKKMGIAAYNAECRKIVMRYSSEWETIVSRTGRW 203
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: D8LVP8_BLAHO (Isoleucine--tRNA ligase n=1 Tax=Blastocystis hominis TaxID=12968 RepID=D8LVP8_BLAHO)

HSP 1 Score: 249 bits (636), Expect = 1.770e-74
Identity = 112/140 (80.00%), Postives = 122/140 (87.14%), Query Frame = 1 
Query:    1 LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            L+  PD LSFP+AE ++L  W EIDAF+TSLKLSEGRP FTFYDGPPFATGLPHYGHILAGTIKDIVTR+AHQTGHYV RRFGWD HGLPVE EIDKKLGI  + DVE MGIA YNAECRGIV+RYC EWE++V RLGRW
Sbjct:    8 LQPAPDNLSFPKAEEEVLKYWNEIDAFKTSLKLSEGRPSFTFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQTGHYVVRRFGWDTHGLPVESEIDKKLGITCSQDVEKMGIAKYNAECRGIVSRYCKEWEAIVNRLGRW 147
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A7R9FT71_9CRUS (Hypothetical protein n=1 Tax=Darwinula stevensoni TaxID=69355 RepID=A0A7R9FT71_9CRUS)

HSP 1 Score: 228 bits (582), Expect = 4.720e-74
Identity = 99/140 (70.71%), Postives = 118/140 (84.29%), Query Frame = 1 
Query:    1 LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            +E+ P+ + FP+ E  ++ +W EIDAFR+ LK S+G+P +TFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQ+G++V RRFGWD HGLPVEYEIDK LGIK ++DVE MGIAAYNAECR IV RY  EW+ V+ RLGRW
Sbjct:    6 VESLPETIDFPKEEEKVMKLWKEIDAFRSCLKQSKGKPRYTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGYHVERRFGWDTHGLPVEYEIDKTLGIKGSEDVEKMGIAAYNAECRKIVMRYAKEWQDVISRLGRW 145
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A1Y3MN45_PIRSE (Uncharacterized protein n=1 Tax=Piromyces sp. (strain E2) TaxID=73868 RepID=A0A1Y3MN45_PIRSE)

HSP 1 Score: 236 bits (603), Expect = 2.820e-72
Identity = 104/140 (74.29%), Postives = 116/140 (82.86%), Query Frame = 1 
Query:    1 LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            L+  P  +SFP++E + L  W EI AF TSLK+SEG+P FTFYDGPPFATGLPHYGH+LAGTIKD+VTRYAHQTGHYV RRFGWDCHGLPVEYEIDKKLGIK  +DV +MGI  YNAECR IV RY  EWE  VKR+GRW
Sbjct:    4 LKDVPSNISFPKSEEETLKFWEEIKAFETSLKMSEGKPAFTFYDGPPFATGLPHYGHLLAGTIKDVVTRYAHQTGHYVERRFGWDCHGLPVEYEIDKKLGIKHKEDVLAMGIDKYNAECRSIVMRYSSEWEHTVKRIGRW 143
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A2G8JIB4_STIJA (Putative isoleucine--tRNA ligase, cytoplasmic n=1 Tax=Stichopus japonicus TaxID=307972 RepID=A0A2G8JIB4_STIJA)

HSP 1 Score: 221 bits (564), Expect = 5.090e-71
Identity = 96/140 (68.57%), Postives = 112/140 (80.00%), Query Frame = 1 
Query:    1 LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            +E  PD ++FPE E ++L +W  +D F+T LK S+G+P +TFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQ G++V RRFGWD HGLPVEYEIDKKLGI    DV+ +GI AYN ECR IV RY  EWE +V RLGRW
Sbjct:    2 VEAVPDHINFPETEEEVLKLWQNLDVFQTCLKQSKGKPRYTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQNGYHVERRFGWDTHGLPVEYEIDKKLGITGPADVQKIGIKAYNDECRKIVMRYSAEWEYIVNRLGRW 141
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A7S3GDZ8_9EUKA (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Palpitomonas bilix TaxID=652834 RepID=A0A7S3GDZ8_9EUKA)

HSP 1 Score: 223 bits (568), Expect = 6.060e-71
Identity = 102/142 (71.83%), Postives = 115/142 (80.99%), Query Frame = 1 
Query:    1 LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLS--EGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            LE  P+R+SFPE E ++   W   +AF+ S++ S  E RP +TFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGH+V RRFGWDCHGLPVEYEID+KLGIKS+DDV  MGI  YNAECR IV RY  EW  VV+RLGRW
Sbjct:   13 LEAVPERVSFPEQEEEVQKHWERTNAFQESVRRSKEENRPVYTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHHVERRFGWDCHGLPVEYEIDQKLGIKSSDDVMKMGIDKYNAECRSIVMRYSTEWRVVVERLGRW 154
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A196S798_BLAHN (Isoleucine--tRNA ligase n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196S798_BLAHN)

HSP 1 Score: 239 bits (610), Expect = 7.480e-71
Identity = 105/140 (75.00%), Postives = 123/140 (87.86%), Query Frame = 1 
Query:    1 LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            L++ PD ++FP+AE ++L  W +IDAF+TSLKLSEG+P+FTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGH+V RRFGWD HGLPVE EIDKKLGI  + DV+ +GIA YNAECRGIV+RY  EWE++V RLGRW
Sbjct:    8 LQSVPDNINFPKAEEEVLKFWNDIDAFQTSLKLSEGKPDFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHHVVRRFGWDTHGLPVECEIDKKLGITCSQDVDKIGIAKYNAECRGIVSRYTKEWEAIVNRLGRW 147
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A433Q1K4_9FUNG (tRNA-synt_1 domain-containing protein n=1 Tax=Jimgerdemannia flammicorona TaxID=994334 RepID=A0A433Q1K4_9FUNG)

HSP 1 Score: 220 bits (561), Expect = 9.260e-71
Identity = 97/134 (72.39%), Postives = 110/134 (82.09%), Query Frame = 1 
Query:   19 RLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            + +FP+ E  ILA W +I+AF TSLKLSEGRP F+F+DGPPFATGLPHYGH+LAGTIKDIVTRYAH TGHYV RRFGWD HGLPVEYE+DK LGIK  DDV +MGI  YNAECR IV RY  EW + V+R+ RW
Sbjct:    9 QFNFPQEEEKILAFWKDINAFHTSLKLSEGRPAFSFFDGPPFATGLPHYGHLLAGTIKDIVTRYAHNTGHYVERRFGWDTHGLPVEYEVDKALGIKGKDDVLAMGIDKYNAECRAIVMRYSREWRTTVERMARW 142
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BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A812UNE2_SYMMI (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812UNE2_SYMMI)

HSP 1 Score: 222 bits (566), Expect = 1.010e-70
Identity = 98/139 (70.50%), Postives = 110/139 (79.14%), Query Frame = 1 
Query:    4 ETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            +T  D+L+F + E +IL  W EIDAF  +LKLSEGRPE+TFYDGPPFATG+PHYGHILAGTIKD VTRYAHQTGH+VSRRFGWDCHGLP+EYEIDK LGI   + V  MG+  YN  CR IV RY  EWE  V RLGRW
Sbjct:    6 DTVSDKLNFSQEEENILEYWREIDAFNLTLKLSEGRPEYTFYDGPPFATGMPHYGHILAGTIKDTVTRYAHQTGHFVSRRFGWDCHGLPIEYEIDKTLGITDRNQVLEMGVGTYNDACRAIVQRYTKEWEKTVTRLGRW 144
[Close]
BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Match: A0A0T6B9P2_9SCAR (tRNA synthetase (Fragment) n=1 Tax=Oryctes borbonicus TaxID=1629725 RepID=A0A0T6B9P2_9SCAR)

HSP 1 Score: 218 bits (554), Expect = 1.200e-70
Identity = 95/136 (69.85%), Postives = 112/136 (82.35%), Query Frame = 1 
Query:   13 PDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLGIKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW 420
            P+ ++F   E   L+ W +IDAF+T LK S+G+P ++FYDGPPFATGLPHYGHILAGTIKD+VTRYAHQ G++V RRFGWDCHGLPVEYEIDK L IK  DDV  MGIAAYN ECR IV+RY GEWE +++RLGRW
Sbjct:   14 PETINFSNEEEITLSHWKKIDAFQTCLKQSKGKPRYSFYDGPPFATGLPHYGHILAGTIKDVVTRYAHQQGYHVDRRFGWDCHGLPVEYEIDKTLNIKGPDDVMKMGIAAYNNECRKIVSRYSGEWEIIMERLGRW 149
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig115809.3275.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1L9U1_NEODS1.760e-7475.00Isoleucine--tRNA ligase (Fragment) n=1 Tax=Neobodo... [more]
D8LVP8_BLAHO1.770e-7480.00Isoleucine--tRNA ligase n=1 Tax=Blastocystis homin... [more]
A0A7R9FT71_9CRUS4.720e-7470.71Hypothetical protein n=1 Tax=Darwinula stevensoni ... [more]
A0A1Y3MN45_PIRSE2.820e-7274.29Uncharacterized protein n=1 Tax=Piromyces sp. (str... [more]
A0A2G8JIB4_STIJA5.090e-7168.57Putative isoleucine--tRNA ligase, cytoplasmic n=1 ... [more]
A0A7S3GDZ8_9EUKA6.060e-7171.83Isoleucine--tRNA ligase (Fragment) n=1 Tax=Palpito... [more]
A0A196S798_BLAHN7.480e-7175.00Isoleucine--tRNA ligase n=1 Tax=Blastocystis sp. s... [more]
A0A433Q1K4_9FUNG9.260e-7172.39tRNA-synt_1 domain-containing protein n=1 Tax=Jimg... [more]
A0A812UNE2_SYMMI1.010e-7070.50Isoleucine--tRNA ligase (Fragment) n=1 Tax=Symbiod... [more]
A0A0T6B9P2_9SCAR1.200e-7069.85tRNA synthetase (Fragment) n=1 Tax=Oryctes borboni... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig115809contigM-pyrifera_M_contig115809:144..563 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score228.4
Seed ortholog evalue2.1e-57
Seed eggNOG ortholog44056.XP_009041958.1
Preferred nameILERS
KEGG rclassRC00055,RC00523
KEGG koko:K01870
KEGG ReactionR03656
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004812,GO:0004822,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006418,GO:0006428,GO:0006518,GO:0006520,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010035,GO:0010038,GO:0010467,GO:0016070,GO:0016874,GO:0016875,GO:0017101,GO:0019538,GO:0019752,GO:0019899,GO:0032991,GO:0034641,GO:0034645,GO:0034660,GO:0042221,GO:0043038,GO:0043039,GO:0043043,GO:0043170,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0046686,GO:0050896,GO:0051020,GO:0071704,GO:0090304,GO:0140098,GO:0140101,GO:1901360,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Belongs to the class-I aminoacyl-tRNA synthetase family
EggNOG OGsCOG0060@1,KOG0434@2759
EC6.1.1.5
COG Functional cat.J
BiGG ReactioniRC1080.CRv4_Au5_s16_g6096_t1
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016
Exons1
Model size420
Cds size420
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815674.5183153-CDS-M-pyrifera_M_contig115809:143..5631622815674.5183153-CDS-M-pyrifera_M_contig115809:143..563Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig115809 144..563 +
1692277516.0373094-CDS-M-pyrifera_M_contig115809:143..5631692277516.0373094-CDS-M-pyrifera_M_contig115809:143..563Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig115809 144..563 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig115809.3275.1prot_M-pyrifera_M_contig115809.3275.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig115809 144..563 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig115809.3275.1

>prot_M-pyrifera_M_contig115809.3275.1 ID=prot_M-pyrifera_M_contig115809.3275.1|Name=mRNA_M-pyrifera_M_contig115809.3275.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=140bp
LETPPDRLSFPEAERDILAMWAEIDAFRTSLKLSEGRPEFTFYDGPPFAT
GLPHYGHILAGTIKDIVTRYAHQTGHYVSRRFGWDCHGLPVEYEIDKKLG
IKSTDDVESMGIAAYNAECRGIVTRYCGEWESVVKRLGRW
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mRNA from alignment at M-pyrifera_M_contig115809:144..563+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig115809.3275.1 ID=mRNA_M-pyrifera_M_contig115809.3275.1|Name=mRNA_M-pyrifera_M_contig115809.3275.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=420bp|location=Sequence derived from alignment at M-pyrifera_M_contig115809:144..563+ (Macrocystis pyrifera P11B4 male)
CTGGAAACCCCACCTGACCGCCTGTCGTTCCCTGAAGCGGAGCGCGACAT
CCTGGCCATGTGGGCCGAGATTGACGCCTTCCGCACGTCGCTGAAGCTGT
CGGAAGGCCGCCCAGAGTTCACGTTCTACGACGGGCCCCCGTTTGCCACC
GGCCTGCCGCACTACGGCCACATCCTGGCCGGCACCATCAAGGACATTGT
CACTCGCTATGCGCACCAGACCGGCCACTACGTCTCCCGCCGCTTCGGCT
GGGACTGTCACGGCCTGCCCGTGGAGTACGAGATTGACAAGAAGCTGGGC
ATCAAGAGCACCGACGATGTTGAGAGCATGGGGATCGCCGCGTACAACGC
CGAGTGCCGCGGCATCGTGACTCGCTATTGCGGCGAGTGGGAGTCTGTGG
TCAAGCGTCTCGGCCGCTGG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig115809:144..563+

>mRNA_M-pyrifera_M_contig115809.3275.1 ID=mRNA_M-pyrifera_M_contig115809.3275.1|Name=mRNA_M-pyrifera_M_contig115809.3275.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=840bp|location=Sequence derived from alignment at M-pyrifera_M_contig115809:144..563+ (Macrocystis pyrifera P11B4 male)
CTGGAAACCCCACCTGACCGCCTGTCGTTCCCTGAAGCGGAGCGCGACAT
CCTGGCCATGTGGGCCGAGATTGACGCCTTCCGCACGTCGCTGAAGCTGT
CGGAAGGCCGCCCAGAGTTCACGTTCTACGACGGGCCCCCGTTTGCCACC
GGCCTGCCGCACTACGGCCACATCCTGGCCGGCACCATCAAGGACATTGT
CACTCGCTATGCGCACCAGACCGGCCACTACGTCTCCCGCCGCTTCGGCT
GGGACTGTCACGGCCTGCCCGTGGAGTACGAGATTGACAAGAAGCTGGGC
ATCAAGAGCACCGACGATGTTGAGAGCATGGGGATCGCCGCGTACAACGC
CGAGTGCCGCGGCATCGTGACTCGCTATTGCGGCGAGTGGGAGTCTGTGG
TCAAGCGTCTCGGCCGCTGGCTGGAAACCCCACCTGACCGCCTGTCGTTC
CCTGAAGCGGAGCGCGACATCCTGGCCATGTGGGCCGAGATTGACGCCTT
CCGCACGTCGCTGAAGCTGTCGGAAGGCCGCCCAGAGTTCACGTTCTACG
ACGGGCCCCCGTTTGCCACCGGCCTGCCGCACTACGGCCACATCCTGGCC
GGCACCATCAAGGACATTGTCACTCGCTATGCGCACCAGACCGGCCACTA
CGTCTCCCGCCGCTTCGGCTGGGACTGTCACGGCCTGCCCGTGGAGTACG
AGATTGACAAGAAGCTGGGCATCAAGAGCACCGACGATGTTGAGAGCATG
GGGATCGCCGCGTACAACGCCGAGTGCCGCGGCATCGTGACTCGCTATTG
CGGCGAGTGGGAGTCTGTGGTCAAGCGTCTCGGCCGCTGG
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