mRNA_M-pyrifera_M_contig11494.3100.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig11494.3100.1
Unique NamemRNA_M-pyrifera_M_contig11494.3100.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: D7G096_ECTSI (Ubiquitin-conjugating enzyme type E2 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G096_ECTSI)

HSP 1 Score: 179 bits (454), Expect = 2.530e-54
Identity = 82/93 (88.17%), Postives = 86/93 (92.47%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMSDFEVHIPDESSMHDFLVAFPGPEDTPY GG+WKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMF    +  +F+
Sbjct:   18 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYQGGVWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFDLVNIFKLFI 110
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A835Z4W6_9STRA (Ubiquitin-conjugating enzyme/RWD-like protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4W6_9STRA)

HSP 1 Score: 161 bits (408), Expect = 2.720e-48
Identity = 71/93 (76.34%), Postives = 83/93 (89.25%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMSDF VHIPDE SMHDF+VAFPGP +TPY GG+WKVHV+LP+EYP++SPSIGFENR++HPNVDEMSGSVCLDVINQTWSPMF    +  +F+
Sbjct:   16 MMSDFAVHIPDECSMHDFMVAFPGPANTPYEGGVWKVHVSLPREYPYRSPSIGFENRIYHPNVDEMSGSVCLDVINQTWSPMFDLVNIFTIFL 108
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A7S2AI11_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2AI11_9STRA)

HSP 1 Score: 156 bits (394), Expect = 1.670e-47
Identity = 70/93 (75.27%), Postives = 80/93 (86.02%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMSD+EV+IPDESSMHDF V F GP DTPY GG+WK+HVTLP  YPFKSPSIGF+NRMFHPNVD++SGSVCLDVINQTWSPMF    +  +F+
Sbjct:   19 MMSDYEVNIPDESSMHDFFVKFEGPPDTPYEGGVWKIHVTLPPAYPFKSPSIGFKNRMFHPNVDDISGSVCLDVINQTWSPMFNLVNIFEVFL 111
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A7S2AI60_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2AI60_9STRA)

HSP 1 Score: 156 bits (394), Expect = 2.260e-46
Identity = 70/93 (75.27%), Postives = 80/93 (86.02%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMSD+EV+IPDESSMHDF V F GP DTPY GG+WK+HVTLP  YPFKSPSIGF+NRMFHPNVD++SGSVCLDVINQTWSPMF    +  +F+
Sbjct:   19 MMSDYEVNIPDESSMHDFFVKFEGPPDTPYEGGVWKIHVTLPPAYPFKSPSIGFKNRMFHPNVDDISGSVCLDVINQTWSPMFNLVNIFEVFL 111
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A7S2W0L0_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2W0L0_9STRA)

HSP 1 Score: 148 bits (374), Expect = 4.810e-43
Identity = 64/93 (68.82%), Postives = 80/93 (86.02%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMSD+EVHIP+E S+H+F V F GP +TPY GG+WKVHV LP++YPFKSPSIGF+NR++HPNVD++SGSVCLDVINQTWSPMF    +  +F+
Sbjct:   31 MMSDYEVHIPNEQSVHEFFVKFHGPAETPYEGGVWKVHVMLPRDYPFKSPSIGFKNRIYHPNVDDLSGSVCLDVINQTWSPMFNLVNIFEIFL 123
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A2D4C6N9_PYTIN (Ubiquitin-conjugating enzyme E2, putative n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C6N9_PYTIN)

HSP 1 Score: 146 bits (368), Expect = 6.840e-43
Identity = 65/93 (69.89%), Postives = 79/93 (84.95%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMS+++VH+ DES  +DF V F GP+DTPY GG+WK+HV+LPKEYPFKSPSIGF NR++HPNVD+MSGSVCLDVINQTWSPMF    +  MF+
Sbjct:   15 MMSNYDVHLTDESRANDFHVKFHGPKDTPYEGGLWKIHVSLPKEYPFKSPSIGFVNRIYHPNVDDMSGSVCLDVINQTWSPMFDLLNIFDMFI 107
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A7S2BA17_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BA17_9STRA)

HSP 1 Score: 147 bits (372), Expect = 3.390e-42
Identity = 66/83 (79.52%), Postives = 73/83 (87.95%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMF 249
            MMSD+EV IPDES MHDF V F GP +TPY  G WK+HVTLP +YPFKSPSIGF+NRMFHPNVD++SGSVCLDVINQTWSPMF
Sbjct:   78 MMSDYEVLIPDESCMHDFFVKFDGPAETPYENGSWKIHVTLPADYPFKSPSIGFKNRMFHPNVDDISGSVCLDVINQTWSPMF 160
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BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A7S3XS74_HETAK (Hypothetical protein n=4 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XS74_HETAK)

HSP 1 Score: 146 bits (368), Expect = 2.120e-41
Identity = 65/83 (78.31%), Postives = 73/83 (87.95%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMF 249
            MMSD+EV +PDE  ++DF VAF GPE TPY GG+W VHVTLP EYP+KSPSIGF NR+FHPNVD+MSGSVCLDVINQTWSPMF
Sbjct:   96 MMSDYEVTLPDEDKIYDFHVAFHGPEKTPYEGGVWDVHVTLPPEYPYKSPSIGFSNRLFHPNVDDMSGSVCLDVINQTWSPMF 178
[Close]
BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: A0A8K1C4X5_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C4X5_PYTOL)

HSP 1 Score: 142 bits (358), Expect = 2.330e-41
Identity = 64/93 (68.82%), Postives = 77/93 (82.80%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV 279
            MMSD+EV + DE+  +DF V F GP +TPY GG+WK+HV+LPKEYPFKSPSIGF NR++HPNVD+MSGSVCLDVINQTWSPMF    V  +F+
Sbjct:   16 MMSDYEVQLTDETKTNDFHVKFHGPAETPYEGGVWKIHVSLPKEYPFKSPSIGFVNRIYHPNVDDMSGSVCLDVINQTWSPMFELVNVFEVFI 108
[Close]
BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Match: H3G861_PHYRM (UBC core domain-containing protein n=31 Tax=Peronosporaceae TaxID=4777 RepID=H3G861_PHYRM)

HSP 1 Score: 140 bits (353), Expect = 1.300e-40
Identity = 62/83 (74.70%), Postives = 71/83 (85.54%), Query Frame = 1 
Query:    1 MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSPSIGFENRMFHPNVDEMSGSVCLDVINQTWSPMF 249
            MMSD+EVH+ DE+   D  + F GP+DTPY GG WK+HVTLPK+YPFKSPSIGF NR++HPNVDE SGSVCLDVINQTWSPMF
Sbjct:   16 MMSDYEVHLTDETKTSDLDIKFNGPKDTPYEGGSWKIHVTLPKDYPFKSPSIGFMNRIYHPNVDETSGSVCLDVINQTWSPMF 98
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig11494.3100.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G096_ECTSI2.530e-5488.17Ubiquitin-conjugating enzyme type E2 n=1 Tax=Ectoc... [more]
A0A835Z4W6_9STRA2.720e-4876.34Ubiquitin-conjugating enzyme/RWD-like protein n=1 ... [more]
A0A7S2AI11_9STRA1.670e-4775.27Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
A0A7S2AI60_9STRA2.260e-4675.27Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7S2W0L0_9STRA4.810e-4368.82Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A2D4C6N9_PYTIN6.840e-4369.89Ubiquitin-conjugating enzyme E2, putative n=1 Tax=... [more]
A0A7S2BA17_9STRA3.390e-4279.52Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S3XS74_HETAK2.120e-4178.31Hypothetical protein n=4 Tax=Heterosigma akashiwo ... [more]
A0A8K1C4X5_PYTOL2.330e-4168.82Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
H3G861_PHYRM1.300e-4074.70UBC core domain-containing protein n=31 Tax=Perono... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig11494contigM-pyrifera_M_contig11494:6410..7401 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score180.6
Seed ortholog evalue3.3e-43
Seed eggNOG ortholog2880.D7G096
Preferred nameUBC4
KEGG koko:K10576
KEGG Pathwayko04120,map04120
Hectar predicted targeting categoryother localisation
GOsGO:0000209,GO:0003674,GO:0003824,GO:0004842,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006109,GO:0006111,GO:0006464,GO:0006508,GO:0006511,GO:0006807,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010498,GO:0010675,GO:0010677,GO:0010906,GO:0016567,GO:0016740,GO:0019222,GO:0019538,GO:0019787,GO:0019899,GO:0019941,GO:0030163,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031625,GO:0032446,GO:0036211,GO:0043161,GO:0043170,GO:0043255,GO:0043412,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044389,GO:0044424,GO:0044444,GO:0044464,GO:0045721,GO:0045912,GO:0048519,GO:0048523,GO:0050789,GO:0050794,GO:0051603,GO:0061630,GO:0061631,GO:0061650,GO:0061659,GO:0062012,GO:0062014,GO:0065007,GO:0070647,GO:0070936,GO:0070979,GO:0071704,GO:0080090,GO:0140096,GO:1901564,GO:1901565,GO:1901575
EggNOG free text desc.ubiquitin protein ligase binding
EggNOG OGsKOG0416@1,KOG0416@2759
EC2.3.2.23
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko04121
Exons2
Model size279
Cds size279
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815665.1040254-CDS-M-pyrifera_M_contig11494:6409..64911622815665.1040254-CDS-M-pyrifera_M_contig11494:6409..6491Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11494 6410..6491 +
1692277511.5786986-CDS-M-pyrifera_M_contig11494:6409..64911692277511.5786986-CDS-M-pyrifera_M_contig11494:6409..6491Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11494 6410..6491 +
1622815665.1181297-CDS-M-pyrifera_M_contig11494:7204..74011622815665.1181297-CDS-M-pyrifera_M_contig11494:7204..7401Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11494 7205..7401 +
1692277511.5990996-CDS-M-pyrifera_M_contig11494:7204..74011692277511.5990996-CDS-M-pyrifera_M_contig11494:7204..7401Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11494 7205..7401 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig11494.3100.1prot_M-pyrifera_M_contig11494.3100.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig11494 6410..7401 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig11494.3100.1

>prot_M-pyrifera_M_contig11494.3100.1 ID=prot_M-pyrifera_M_contig11494.3100.1|Name=mRNA_M-pyrifera_M_contig11494.3100.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=93bp
MMSDFEVHIPDESSMHDFLVAFPGPEDTPYTGGIWKVHVTLPKEYPFKSP
SIGFENRMFHPNVDEMSGSVCLDVINQTWSPMFGASYVALMFV
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mRNA from alignment at M-pyrifera_M_contig11494:6410..7401+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig11494.3100.1 ID=mRNA_M-pyrifera_M_contig11494.3100.1|Name=mRNA_M-pyrifera_M_contig11494.3100.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=992bp|location=Sequence derived from alignment at M-pyrifera_M_contig11494:6410..7401+ (Macrocystis pyrifera P11B4 male)
ATGATGAGCGACTTCGAGGTTCATATCCCAGATGAATCGAGCATGCACGA
CTTCCTCGTCGCCTTCCCCGGGCCCGAAGACAGTAAGTGGTAACGAAGTA
AAGCACCGTTCGTCCCGAAGACCCAACTGCCATGCGCCGGTCTGAAGCAA
AAGGCTGTCTCTTGCCAGCAAGCAGGCGGGGCCCTTTTTCAGCTCTCATC
ATTTTCCATTTTTTTTTGTCTAGGGGCGGAACTGACTCATCTAAGGGGCT
GTCGCCCGCGGGCGCTGTGTTGCATGCTTCCCGGCCAGCTCCGCGCCTTG
AACACCCTCACGAGGATCCCGAACTCCCCGCGCCAACGTGTGTCGTGTTC
GCCTCTTTTCTTCCTTCCGTGTGCACAACACGTTTTTTCCGACGCTTCAT
CAAAAAAGTACGACTAAAACACGAGCAGAAACAAAATGGCCCAATCGCCA
CATCTCTTTTTCTCGTACAAAAAATATGTATGATATCACGTTATTATCCG
TACTATCAATACAAAAGTACCCCAAGTGTGTTGCGATGAAAACCAATTCG
AAGTCCGAGAGATTCTTCGATATTCATCTGTATTATCACCAGTACAGGTA
TACTCGCACTCGTACATAAAACAACCCCCACCCCTCCGGATGCGCCGGAT
GGGTCATGTTATTTTAGTTTATTCATTATTTATCGAACCCATCTCTGCGC
AGGTACGTACTTGATACGAAAGACTTCTTGTCCCGTCCCTCCTCCCGTCT
TCCCATATCACCACCCACGGCATTGTTTCGCGCTCGCGCTCGCAGCTCCG
TACACAGGAGGTATCTGGAAGGTGCACGTTACGCTGCCGAAGGAGTACCC
TTTCAAGTCTCCATCGATTGGGTTTGAGAACCGCATGTTCCACCCGAACG
TGGATGAGATGTCGGGCTCTGTTTGCCTGGACGTGATCAACCAGACCTGG
AGCCCAATGTTCGGTGCGTCGTACGTGGCTTTGATGTTTGTT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig11494:6410..7401+

>mRNA_M-pyrifera_M_contig11494.3100.1 ID=mRNA_M-pyrifera_M_contig11494.3100.1|Name=mRNA_M-pyrifera_M_contig11494.3100.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=558bp|location=Sequence derived from alignment at M-pyrifera_M_contig11494:6410..7401+ (Macrocystis pyrifera P11B4 male)
ATGATGAGCGACTTCGAGGTTCATATCCCAGATGAATCGAGCATGCACGA
CTTCCTCGTCGCCTTCCCCGGGCCCGAAGACAATGATGAGCGACTTCGAG
GTTCATATCCCAGATGAATCGAGCATGCACGACTTCCTCGTCGCCTTCCC
CGGGCCCGAAGACACTCCGTACACAGGAGGTATCTGGAAGGTGCACGTTA
CGCTGCCGAAGGAGTACCCTTTCAAGTCTCCATCGATTGGGTTTGAGAAC
CGCATGTTCCACCCGAACGTGGATGAGATGTCGGGCTCTGTTTGCCTGGA
CGTGATCAACCAGACCTGGAGCCCAATGTTCGGTGCGTCGTACGTGGCTT
TGATGTTTGTTCTCCGTACACAGGAGGTATCTGGAAGGTGCACGTTACGC
TGCCGAAGGAGTACCCTTTCAAGTCTCCATCGATTGGGTTTGAGAACCGC
ATGTTCCACCCGAACGTGGATGAGATGTCGGGCTCTGTTTGCCTGGACGT
GATCAACCAGACCTGGAGCCCAATGTTCGGTGCGTCGTACGTGGCTTTGA
TGTTTGTT
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