mRNA_M-pyrifera_M_contig114290.2974.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_M-pyrifera_M_contig114290.2974.1
Unique Name	mRNA_M-pyrifera_M_contig114290.2974.1
Type	mRNA
Organism	Macrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))

Homology

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A0P0W476_ORYSJ (Os03g0800100 protein n=3 Tax=Oryza TaxID=4527 RepID=A0A0P0W476_ORYSJ)

HSP 1 Score: 174 bits (440), Expect = 3.790e-50
Identity = 92/164 (56.10%), Postives = 127/164 (77.44%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGD NL + FVW+KK++ +  +G+KVD S KGEK ++ E   EI K K RREER +EK   EE+ ALL RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPIDV+ KNL    EFD +++EPY VF+GLT++E+ +L +DI+M+L+LD
Sbjct:  116 VSGYSNDSNPFGDPNLTEKFVWRKKIERDVSQGQKVDISVKGEKKKQRERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKKEEEFHFDQSKVRSEIRLREGRTKPIDVLLKNLNFSEEFDVELNEPYLVFKGLTVKEMEELRDDIKMHLDLD 279

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A453M3U4_AEGTS (Cactin_mid domain-containing protein n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A453M3U4_AEGTS)

HSP 1 Score: 171 bits (433), Expect = 1.040e-48
Identity = 93/164 (56.71%), Postives = 125/164 (76.22%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGD NL + FVW+KK+     EG+KVD S K EK R+ E   EI K K RREER +EK   EE+ ALL RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPIDV+ KNL    EFD +++EPY VF+GLT++E+ +L++DI+M+L+LD
Sbjct:  136 VSGYSNDSNPFGDPNLTENFVWRKKIDRAVTEGQKVDISVKAEKKRQRERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKKEEEFHFDQSKFRSEIRLREGRTKPIDVLLKNLNFADEFDVELNEPYLVFKGLTVKEMEELHDDIKMHLDLD 299

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A3ANP5_ORYSJ (Uncharacterized protein OSJNBa0052F07.12 n=17 Tax=Oryzeae TaxID=147380 RepID=A3ANP5_ORYSJ)

HSP 1 Score: 174 bits (440), Expect = 6.150e-48
Identity = 92/164 (56.10%), Postives = 127/164 (77.44%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGD NL + FVW+KK++ +  +G+KVD S KGEK ++ E   EI K K RREER +EK   EE+ ALL RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPIDV+ KNL    EFD +++EPY VF+GLT++E+ +L +DI+M+L+LD
Sbjct:  116 VSGYSNDSNPFGDPNLTEKFVWRKKIERDVSQGQKVDISVKGEKKKQRERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKKEEEFHFDQSKVRSEIRLREGRTKPIDVLLKNLNFSEEFDVELNEPYLVFKGLTVKEMEELRDDIKMHLDLD 279

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A5P1FBI7_ASPOF (Uncharacterized protein n=3 Tax=Asparagus officinalis TaxID=4686 RepID=A0A5P1FBI7_ASPOF)

HSP 1 Score: 173 bits (439), Expect = 1.360e-47
Identity = 89/164 (54.27%), Postives = 131/164 (79.88%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGDSNL +TFVW+KK++ +  +GR++D S K EK R++E   EI K K RREER +EK   EE+ ALL RER  A+FQDWE++E EFH+ Q++ RS+IR+REGR KPID++ KNL +  +FD +++EPY VF+GLT++E+ +L++DI+M+L+LD
Sbjct:  141 VSGYSNDSNPFGDSNLGETFVWRKKIERDVVQGRELDISVKAEKKRQKERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKREEEFHFDQSKVRSQIRLREGRMKPIDILSKNLNVSDDFDIEINEPYTVFKGLTVKEMEELHDDIKMHLDLD 304

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A6P5FRK7_ANACO (cactin n=3 Tax=Ananas comosus TaxID=4615 RepID=A0A6P5FRK7_ANACO)

HSP 1 Score: 172 bits (435), Expect = 3.160e-47
Identity = 90/164 (54.88%), Postives = 126/164 (76.83%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGD+NL + FVW+KK++ +  +GR VD S K EK R+ E   EI K K RREER +EK   EE+ A+L RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPIDV+ KNL    +FD +++EPY VF+GLT++E+ +L +DI+M+L+LD
Sbjct:  119 VSGYSNDSNPFGDANLTEKFVWRKKIERDVAQGRPVDISVKSEKKRQRERMAEIEKVKKRREERAIEKAQHEEEMAMLARERARAEFQDWEKKEEEFHFDQSKVRSEIRLREGRTKPIDVLLKNLNFSEDFDIEINEPYMVFKGLTVKEMEELQDDIKMHLDLD 282

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: I1GM40_BRADI (Uncharacterized protein n=28 Tax=Pooideae TaxID=147368 RepID=I1GM40_BRADI)

HSP 1 Score: 171 bits (433), Expect = 5.440e-47
Identity = 93/164 (56.71%), Postives = 124/164 (75.61%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGD NL + FVW+KK++    EG KVD S K EK R+ E   EI K K RREER +EK   EE+ ALL RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPIDV+ KNL    EFD +++EPY VF+GLT++E+ +L +DI+M+L+LD
Sbjct:  111 VSGYSNDSNPFGDPNLTENFVWRKKIERAVTEGEKVDISVKSEKKRQRERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKKEEEFHFDQSKFRSEIRLREGRTKPIDVLLKNLNFADEFDVELNEPYLVFKGLTVKEMEELRDDIKMHLDLD 274

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: K4A6Q4_SETIT (Uncharacterized protein n=20 Tax=PACMAD clade TaxID=147370 RepID=K4A6Q4_SETIT)

HSP 1 Score: 170 bits (430), Expect = 1.590e-46
Identity = 91/164 (55.49%), Postives = 125/164 (76.22%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGD NL + FVW+KK++ +  +G+KVD S K EK R+ E   EI K K RREER +EK   EE+  LL RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPIDV+ KNL    EFD +++EPY VF+GLT++E+ +L +DI+M+L+LD
Sbjct:  121 VSGYSNDSNPFGDPNLTENFVWRKKIERDVSQGQKVDISVKSEKKRQLERMAEIEKVKKRREERAIEKAQHEEEMQLLARERARAEFQDWEKKEEEFHFDQSKVRSEIRLREGRTKPIDVLLKNLSFSDEFDVELNEPYLVFKGLTVKEMEELRDDIKMHLDLD 284

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A8J5X6M7_ZIZPA (Cactin_mid domain-containing protein n=1 Tax=Zizania palustris TaxID=103762 RepID=A0A8J5X6M7_ZIZPA)

HSP 1 Score: 166 bits (419), Expect = 5.240e-46
Identity = 87/164 (53.05%), Postives = 124/164 (75.61%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GY +++NPFGD NL + FVW+KK++ +  +G+KVD S K EK ++ E   EI K K RREER +EK   EE+ ALL RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPID++ KNL    EFD +++EPY VF+GL ++++ +L +DI+M+L+LD
Sbjct:  110 VSGYCNDSNPFGDPNLTEKFVWRKKIERDVSQGQKVDISVKSEKKKQRERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKKEEEFHFDQSKVRSEIRLREGRTKPIDILLKNLNFTEEFDVELNEPYLVFKGLAVKDMEELRDDIKMHLDLD 273

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BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A2I0AFL2_9ASPA (Uncharacterized protein n=1 Tax=Apostasia shenzhenica TaxID=1088818 RepID=A0A2I0AFL2_9ASPA)

HSP 1 Score: 167 bits (424), Expect = 1.290e-45
Identity = 88/164 (53.66%), Postives = 124/164 (75.61%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGDSNL + FVW+KK++ +  +G  VD S   EK R+ E   EI K K RREER +EK   EE+ A+L RER  A+FQDWE+KE EFH+ Q++ RS+IR+REGR KPIDV+ KNL    +FD +++EPY VF+GLT++E+ +L +DI+MYL++D
Sbjct:  137 VSGYSNDSNPFGDSNLTEKFVWRKKIERDVSQGVPVDISVSSEKKRQRERMAEIEKVKKRREERAIEKAQHEEEMAMLARERARAEFQDWEKKEEEFHFDQSKVRSQIRLREGRVKPIDVLAKNLNCSDDFDIEINEPYMVFKGLTVKEMEELRDDIKMYLDMD 300

[Close]

BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Match: A0A6I9RVZ9_ELAGV (cactin n=2 Tax=Arecaceae TaxID=4710 RepID=A0A6I9RVZ9_ELAGV)

HSP 1 Score: 167 bits (422), Expect = 2.170e-45
Identity = 87/164 (53.05%), Postives = 127/164 (77.44%), Query Frame = 1

Query:    1 LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEELQREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYKQARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEELVQLNEDIRMYLELD 492
            ++GYS+++NPFGDSNL + FVW+KK++ +  +G+ +D S K EK R+ E   EI K K RREER +EK   EE+ A+L RER  A+FQDWE+KE EFH+ Q++ RSEIR+REGR KPID++ KNL    +FD +++EPY VF+GLT++E+ +L++DI+M+L++D
Sbjct:  121 VSGYSNDSNPFGDSNLTEKFVWRKKIERDVAQGQPLDISVKAEKKRQRERMAEIEKVKKRREERAIEKAQHEEEMAMLARERARAEFQDWEKKEEEFHFDQSKIRSEIRLREGRIKPIDILSKNLNGSEDFDIEINEPYMVFKGLTVKEMEELHDDIKMHLDMD 284

The following BLAST results are available for this feature:

BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot

BLAST of mRNA_M-pyrifera_M_contig114290.2974.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A0A0P0W476_ORYSJ	3.790e-50	56.10	Os03g0800100 protein n=3 Tax=Oryza TaxID=4527 RepI...	[more]
A0A453M3U4_AEGTS	1.040e-48	56.71	Cactin_mid domain-containing protein n=1 Tax=Aegil...	[more]
A3ANP5_ORYSJ	6.150e-48	56.10	Uncharacterized protein OSJNBa0052F07.12 n=17 Tax=...	[more]
A0A5P1FBI7_ASPOF	1.360e-47	54.27	Uncharacterized protein n=3 Tax=Asparagus officina...	[more]
A0A6P5FRK7_ANACO	3.160e-47	54.88	cactin n=3 Tax=Ananas comosus TaxID=4615 RepID=A0A...	[more]
I1GM40_BRADI	5.440e-47	56.71	Uncharacterized protein n=28 Tax=Pooideae TaxID=14...	[more]
K4A6Q4_SETIT	1.590e-46	55.49	Uncharacterized protein n=20 Tax=PACMAD clade TaxI...	[more]
A0A8J5X6M7_ZIZPA	5.240e-46	53.05	Cactin_mid domain-containing protein n=1 Tax=Zizan...	[more]
A0A2I0AFL2_9ASPA	1.290e-45	53.66	Uncharacterized protein n=1 Tax=Apostasia shenzhen...	[more]
A0A6I9RVZ9_ELAGV	2.170e-45	53.05	cactin n=2 Tax=Arecaceae TaxID=4710 RepID=A0A6I9RV...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
M-pyrifera_M_contig114290	contig	M-pyrifera_M_contig114290:59..550 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90	2022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male	2021-02-24

Properties


Property Name	Value
Taxonomic scope	Viridiplantae
Seed ortholog score	175.3
Seed ortholog evalue	2.4e-41
Seed eggNOG ortholog	40148.OGLUM03G37380.1
Hectar predicted targeting category	other localisation
EggNOG free text desc.	Conserved mid region of cactin
EggNOG OGs	37MJ3@33090,3G77I@35493,3I2J2@38820,3KQGI@4447,KOG2370@1,KOG2370@2759
COG Functional cat.	T
Best tax level	Poales
Best eggNOG OG	NA\|NA\|NA
Exons	1
Model size	492
Cds size	492
Stop	0
Start	0

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622815658.9499211-CDS-M-pyrifera_M_contig114290:58..550	1622815658.9499211-CDS-M-pyrifera_M_contig114290:58..550	Macrocystis pyrifera P11B4 male	CDS	M-pyrifera_M_contig114290 59..550 +
1692277508.8616586-CDS-M-pyrifera_M_contig114290:58..550	1692277508.8616586-CDS-M-pyrifera_M_contig114290:58..550	Macrocystis pyrifera P11B4 male	CDS	M-pyrifera_M_contig114290 59..550 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_M-pyrifera_M_contig114290.2974.1	prot_M-pyrifera_M_contig114290.2974.1	Macrocystis pyrifera P11B4 male	polypeptide	M-pyrifera_M_contig114290 59..550 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at M-pyrifera_M_contig114290:59..550+
coding sequence from alignment at M-pyrifera_M_contig114290:59..550+

protein sequence of mRNA_M-pyrifera_M_contig114290.2974.1

>prot_M-pyrifera_M_contig114290.2974.1 ID=prot_M-pyrifera_M_contig114290.2974.1|Name=mRNA_M-pyrifera_M_contig114290.2974.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=164bp
LAGYSDETNPFGDSNLAQTFVWKKKVQSEREEGRKVDTSRKGEKARKEEL
QREILKAKSRREEREMEKQLMEEQRALLDRERNLAQFQDWEEKEIEFHYK
QARKRSEIRIREGRAKPIDVIYKNLQLDPEFDFDVHEPYEVFRGLTLEEL
VQLNEDIRMYLELD

mRNA from alignment at M-pyrifera_M_contig114290:59..550+

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_M-pyrifera_M_contig114290.2974.1 ID=mRNA_M-pyrifera_M_contig114290.2974.1|Name=mRNA_M-pyrifera_M_contig114290.2974.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=492bp|location=Sequence derived from alignment at M-pyrifera_M_contig114290:59..550+ (Macrocystis pyrifera P11B4 male)
CTGGCAGGCTACAGCGACGAAACGAATCCTTTTGGCGACTCGAACCTCGC
GCAGACCTTCGTCTGGAAGAAGAAGGTCCAGAGCGAGCGCGAGGAGGGGC
GGAAGGTGGACACCTCGAGGAAGGGAGAGAAGGCGAGGAAGGAGGAGCTC
CAGAGGGAGATCCTCAAAGCCAAGAGCCGCAGAGAGGAGCGAGAGATGGA
GAAGCAGCTGATGGAGGAGCAGAGGGCGCTGCTGGACCGCGAGAGGAATC
TCGCGCAGTTCCAGGACTGGGAGGAGAAGGAGATCGAGTTCCACTACAAG
CAGGCCCGCAAGAGGAGCGAGATCCGCATCAGGGAGGGCAGGGCCAAGCC
GATCGACGTCATCTACAAGAACCTCCAGCTCGACCCAGAGTTCGACTTTG
ACGTGCACGAGCCCTACGAGGTGTTCAGGGGCCTCACCCTCGAGGAGCTG
GTGCAGCTCAACGAGGACATCCGCATGTACCTCGAGCTGGAC

Coding sequence (CDS) from alignment at M-pyrifera_M_contig114290:59..550+

>mRNA_M-pyrifera_M_contig114290.2974.1 ID=mRNA_M-pyrifera_M_contig114290.2974.1|Name=mRNA_M-pyrifera_M_contig114290.2974.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=984bp|location=Sequence derived from alignment at M-pyrifera_M_contig114290:59..550+ (Macrocystis pyrifera P11B4 male)
CTGGCAGGCTACAGCGACGAAACGAATCCTTTTGGCGACTCGAACCTCGC
GCAGACCTTCGTCTGGAAGAAGAAGGTCCAGAGCGAGCGCGAGGAGGGGC
GGAAGGTGGACACCTCGAGGAAGGGAGAGAAGGCGAGGAAGGAGGAGCTC
CAGAGGGAGATCCTCAAAGCCAAGAGCCGCAGAGAGGAGCGAGAGATGGA
GAAGCAGCTGATGGAGGAGCAGAGGGCGCTGCTGGACCGCGAGAGGAATC
TCGCGCAGTTCCAGGACTGGGAGGAGAAGGAGATCGAGTTCCACTACAAG
CAGGCCCGCAAGAGGAGCGAGATCCGCATCAGGGAGGGCAGGGCCAAGCC
GATCGACGTCATCTACAAGAACCTCCAGCTCGACCCAGAGTTCGACTTTG
ACGTGCACGAGCCCTACGAGGTGTTCAGGGGCCTCACCCTCGAGGAGCTG
GTGCAGCTCAACGAGGACATCCGCATGTACCTCGAGCTGGACCTGGCAGG
CTACAGCGACGAAACGAATCCTTTTGGCGACTCGAACCTCGCGCAGACCT
TCGTCTGGAAGAAGAAGGTCCAGAGCGAGCGCGAGGAGGGGCGGAAGGTG
GACACCTCGAGGAAGGGAGAGAAGGCGAGGAAGGAGGAGCTCCAGAGGGA
GATCCTCAAAGCCAAGAGCCGCAGAGAGGAGCGAGAGATGGAGAAGCAGC
TGATGGAGGAGCAGAGGGCGCTGCTGGACCGCGAGAGGAATCTCGCGCAG
TTCCAGGACTGGGAGGAGAAGGAGATCGAGTTCCACTACAAGCAGGCCCG
CAAGAGGAGCGAGATCCGCATCAGGGAGGGCAGGGCCAAGCCGATCGACG
TCATCTACAAGAACCTCCAGCTCGACCCAGAGTTCGACTTTGACGTGCAC
GAGCCCTACGAGGTGTTCAGGGGCCTCACCCTCGAGGAGCTGGTGCAGCT
CAACGAGGACATCCGCATGTACCTCGAGCTGGAC