mRNA_M-pyrifera_M_contig113193.2748.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig113193.2748.1
Unique NamemRNA_M-pyrifera_M_contig113193.2748.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: UPI0016650325 (AMP-binding protein n=1 Tax=Chelatococcus reniformis TaxID=1494448 RepID=UPI0016650325)

HSP 1 Score: 260 bits (664), Expect = 2.910e-82
Identity = 127/155 (81.94%), Postives = 136/155 (87.74%), Query Frame = 1 
Query:    1 TEFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            T+FEYFNDPAKTAE  SADG+M++VGDVGYVDDDG+LYLTDRATFMIISGGVNIYPQE ENLLITHP VADAAVFGVPNEDLGEEVKAVVQ MPG+   DE   EL+ FC EHL+RQK PRSIDFE ELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  362 TQFEYFNDPAKTAETRSADGSMSTVGDVGYVDDDGYLYLTDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNEDLGEEVKAVVQAMPGIEAGDELGRELMAFCAEHLARQKCPRSIDFEEELPRLPTGKLYKRLLRDRYWGNRTSRIV 516
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BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A533J7A0_9BRAD (Acyl-CoA synthetase (Fragment) n=1 Tax=Bradyrhizobium icense TaxID=1274631 RepID=A0A533J7A0_9BRAD)

HSP 1 Score: 257 bits (657), Expect = 1.350e-81
Identity = 128/153 (83.66%), Postives = 135/153 (88.24%), Query Frame = 1 
Query:    7 FEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            FEYFNDPAKT EA SAD TM++VGDVGYVDDD FLYLTDRATFMIISGGVNIYPQE ENLLITHP +ADAAVFGVPN DLGEEVKAVVQ MPGVA S+E  AEL+ FC + LSRQKVPRSIDFEAELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  330 FEYFNDPAKTKEARSADETMSTVGDVGYVDDDNFLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAVFGVPNADLGEEVKAVVQPMPGVAPSEELAAELIAFCNQSLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWGNKTSRIV 482
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BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A259MM58_9PROT (Acyl-CoA synthetase n=4 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A259MM58_9PROT)

HSP 1 Score: 256 bits (655), Expect = 7.200e-81
Identity = 125/155 (80.65%), Postives = 138/155 (89.03%), Query Frame = 1 
Query:    1 TEFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            T FEYFNDPAKT EA S DGTM++VGDVGYVD DG+L+LTDRATFMIISGGVNIYPQE ENLLITHP VADAAVFGVPN DLGEEVKAVVQLMPGV ++  T  EL+ FCG+HL+RQKVPRS+DFE ELPRLPTGKLYKR LRDRYWG+ +SRI+
Sbjct:  366 TPFEYFNDPAKTREARSEDGTMSTVGDVGYVDADGYLHLTDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNADLGEEVKAVVQLMPGVPKTGATAEELIAFCGQHLARQKVPRSVDFEDELPRLPTGKLYKRLLRDRYWGDKKSRIV 520
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BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: UPI0016688D22 (AMP-binding protein n=1 Tax=Chelatococcus reniformis TaxID=1494448 RepID=UPI0016688D22)

HSP 1 Score: 256 bits (654), Expect = 9.180e-81
Identity = 124/153 (81.05%), Postives = 131/153 (85.62%), Query Frame = 1 
Query:    7 FEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            FEYFNDP KTAE  S DGTMT+VGDVGYVDDD +LYLTDRATFMIISGGVNIYPQE ENLLITHP VADAAVFGVPNEDLGEEVKAVVQ MPG+   DE   EL+ FC EHL+RQK PRS+DFE ELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  364 FEYFNDPEKTAETRSTDGTMTTVGDVGYVDDDNYLYLTDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNEDLGEEVKAVVQAMPGIEAGDELARELMAFCAEHLARQKCPRSVDFEEELPRLPTGKLYKRLLRDRYWGNRTSRIV 516
[Close]
BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A2S8LDM4_9MYCO (Acyl-CoA synthetase n=1 Tax=Mycobacterium sp. ITM-2016-00318 TaxID=2099693 RepID=A0A2S8LDM4_9MYCO)

HSP 1 Score: 256 bits (653), Expect = 1.300e-80
Identity = 125/154 (81.17%), Postives = 135/154 (87.66%), Query Frame = 1 
Query:    4 EFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            +F YFNDP KTAE+ SADGTMT+VGDVGYVDDDG+LYLTDRATFMIISGGVNIYPQE ENLLITHP VADAAVFGVPN+DLGEEVKAVVQ MPG+    +   ELL FC EHLSRQKVPRSIDFEAELPRLPTGKLYKR LRDRYW +  +RI+
Sbjct:  363 DFSYFNDPQKTAESRSADGTMTTVGDVGYVDDDGYLYLTDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNDDLGEEVKAVVQPMPGIDAGQDFADELLTFCSEHLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWNDHATRIV 516
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BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: UPI001BADAD7F (AMP-binding protein n=1 Tax=Bradyrhizobium sp. AUGA SZCCT0283 TaxID=2807671 RepID=UPI001BADAD7F)

HSP 1 Score: 255 bits (652), Expect = 1.830e-80
Identity = 126/153 (82.35%), Postives = 134/153 (87.58%), Query Frame = 1 
Query:    7 FEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            FEYFNDPAKT EA SADGTM++VGDVGYVDDDG+LYLTDRATFMIISGGVNIYPQE ENLLITHP +ADAAVFGVPN DLGEEVKAVVQ MPGV+   E   EL+ FC + LSRQKVPRSIDFEAELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  364 FEYFNDPAKTQEARSADGTMSTVGDVGYVDDDGYLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAVFGVPNPDLGEEVKAVVQPMPGVSPGPELADELIAFCSQSLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWGNKTSRIV 516
[Close]
BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A0R3KUZ4_9BRAD (Acyl-CoA synthetase n=19 Tax=Bradyrhizobium TaxID=374 RepID=A0A0R3KUZ4_9BRAD)

HSP 1 Score: 255 bits (651), Expect = 2.580e-80
Identity = 126/155 (81.29%), Postives = 134/155 (86.45%), Query Frame = 1 
Query:    1 TEFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            T FEYFNDP KT EA SADGTM++VGDVGYVDDDG+LYLTDRATFMIISGGVNIYPQE ENLLITHP +ADAAVFGVPN DLGEEVKAVVQ MPGV+   E   EL+ FC + LSRQKVPRSIDFEAELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  362 TPFEYFNDPVKTQEARSADGTMSTVGDVGYVDDDGYLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAVFGVPNPDLGEEVKAVVQPMPGVSPGQELADELIAFCSQSLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWGNKTSRIV 516
[Close]
BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A1M7A877_9BRAD (Long-chain acyl-CoA synthetase n=2 Tax=Bradyrhizobium TaxID=374 RepID=A0A1M7A877_9BRAD)

HSP 1 Score: 253 bits (647), Expect = 1.140e-79
Identity = 125/155 (80.65%), Postives = 135/155 (87.10%), Query Frame = 1 
Query:    1 TEFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            T FEYF DPAKTAE+ S+DG+M++VGDVGYVDDDG+LYLTDRATFMIISGGVNIYPQE ENLLITHP +ADAAVFGVPN DLGEEVKAVVQ MPGVA   E   EL+ FC + LSRQKVPRSIDFEAELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  366 TPFEYFGDPAKTAESRSSDGSMSTVGDVGYVDDDGYLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAVFGVPNPDLGEEVKAVVQPMPGVAPGQELADELIAFCSQSLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWGNKTSRIV 520
[Close]
BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A1B1UQ57_9BRAD (Acyl-CoA synthetase n=12 Tax=Bradyrhizobiaceae TaxID=41294 RepID=A0A1B1UQ57_9BRAD)

HSP 1 Score: 252 bits (643), Expect = 4.080e-79
Identity = 125/155 (80.65%), Postives = 132/155 (85.16%), Query Frame = 1 
Query:    1 TEFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            T FEYFNDP KT EA S DG+M++VGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQE ENLLITHP +ADAAVFGVPN DLGEEVKAVVQ MPGV+   E   EL+ FC   LSRQKVPRSIDFEAELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:  362 TPFEYFNDPGKTQEARSPDGSMSTVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAVFGVPNPDLGEEVKAVVQPMPGVSPGQELADELIAFCSRSLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWGNKTSRIV 516
[Close]
BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Match: A0A1Y6KPI5_9BRAD (Putative AMP-dependent synthetase putative long-chain-fatty-acid--CoA ligase (Acyl-CoA synthetase) n=2 Tax=Bradyrhizobium sp. ORS 285 TaxID=115808 RepID=A0A1Y6KPI5_9BRAD)

HSP 1 Score: 242 bits (618), Expect = 4.450e-79
Identity = 121/153 (79.08%), Postives = 129/153 (84.31%), Query Frame = 1 
Query:    7 FEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISGGVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESDETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEGQSRIL 465
            FEYFNDP +T EA SADGTM++VGDVGYVD DG+LYLTDRATFMIISGGVNIYPQE ENLLITHP VADAAVFGVPN DLGEEVKAV+Q M GV    E   EL+ FC + LSRQKVPRSIDFE ELPRLPTGKLYKR LRDRYWG   SRI+
Sbjct:   70 FEYFNDPERTREARSADGTMSTVGDVGYVDADGYLYLTDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNPDLGEEVKAVIQPMSGVMPGPELEEELIAFCRQSLSRQKVPRSIDFEKELPRLPTGKLYKRLLRDRYWGNKTSRIV 222
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig113193.2748.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00166503252.910e-8281.94AMP-binding protein n=1 Tax=Chelatococcus reniform... [more]
A0A533J7A0_9BRAD1.350e-8183.66Acyl-CoA synthetase (Fragment) n=1 Tax=Bradyrhizob... [more]
A0A259MM58_9PROT7.200e-8180.65Acyl-CoA synthetase n=4 Tax=Alphaproteobacteria Ta... [more]
UPI0016688D229.180e-8181.05AMP-binding protein n=1 Tax=Chelatococcus reniform... [more]
A0A2S8LDM4_9MYCO1.300e-8081.17Acyl-CoA synthetase n=1 Tax=Mycobacterium sp. ITM-... [more]
UPI001BADAD7F1.830e-8082.35AMP-binding protein n=1 Tax=Bradyrhizobium sp. AUG... [more]
A0A0R3KUZ4_9BRAD2.580e-8081.29Acyl-CoA synthetase n=19 Tax=Bradyrhizobium TaxID=... [more]
A0A1M7A877_9BRAD1.140e-7980.65Long-chain acyl-CoA synthetase n=2 Tax=Bradyrhizob... [more]
A0A1B1UQ57_9BRAD4.080e-7980.65Acyl-CoA synthetase n=12 Tax=Bradyrhizobiaceae Tax... [more]
A0A1Y6KPI5_9BRAD4.450e-7979.08Putative AMP-dependent synthetase putative long-ch... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig113193contigM-pyrifera_M_contig113193:112..579 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score253.8
Seed ortholog evalue5.1e-65
Seed eggNOG ortholog1038859.AXAU01000007_gene5767
Preferred nameMA20_22915
KEGG rclassRC00004,RC00014
KEGG koko:K00666,ko:K01897
KEGG TC4.C.1.1
KEGG ReactionR01280
KEGG Pathwayko00061,ko00071,ko01100,ko01212,ko02024,ko03320,ko04146,ko04216,ko04714,ko04920,map00061,map00071,map01100,map01212,map02024,map03320,map04146,map04216,map04714,map04920
KEGG ModuleM00086
Hectar predicted targeting categoryother localisation
EggNOG free text desc.AMP-binding enzyme C-terminal domain
EggNOG OGs1MUIC@1224,2TT3K@28211,3JV64@41294,COG0318@1,COG0318@2
EC6.2.1.3
COG Functional cat.IQ
Best tax levelBradyrhizobiaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01004,ko04147
Exons1
Model size468
Cds size468
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815646.6749532-CDS-M-pyrifera_M_contig113193:111..5791622815646.6749532-CDS-M-pyrifera_M_contig113193:111..579Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig113193 112..579 -
1692277503.522877-CDS-M-pyrifera_M_contig113193:111..5791692277503.522877-CDS-M-pyrifera_M_contig113193:111..579Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig113193 112..579 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig113193.2748.1prot_M-pyrifera_M_contig113193.2748.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig113193 112..579 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig113193.2748.1

>prot_M-pyrifera_M_contig113193.2748.1 ID=prot_M-pyrifera_M_contig113193.2748.1|Name=mRNA_M-pyrifera_M_contig113193.2748.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=156bp
TEFEYFNDPAKTAEATSADGTMTSVGDVGYVDDDGFLYLTDRATFMIISG
GVNIYPQETENLLITHPSVADAAVFGVPNEDLGEEVKAVVQLMPGVAESD
ETVAELLRFCGEHLSRQKVPRSIDFEAELPRLPTGKLYKRTLRDRYWGEG
QSRIL*
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mRNA from alignment at M-pyrifera_M_contig113193:112..579-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig113193.2748.1 ID=mRNA_M-pyrifera_M_contig113193.2748.1|Name=mRNA_M-pyrifera_M_contig113193.2748.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=468bp|location=Sequence derived from alignment at M-pyrifera_M_contig113193:112..579- (Macrocystis pyrifera P11B4 male)
ACCGAGTTCGAGTACTTCAACGATCCGGCGAAGACGGCCGAAGCCACGTC
GGCCGACGGCACGATGACCAGCGTCGGCGATGTCGGGTATGTCGACGACG
ACGGGTTTCTCTACCTGACCGACCGGGCGACTTTCATGATCATCTCGGGC
GGAGTCAACATCTACCCGCAAGAGACCGAGAACCTCCTCATCACCCACCC
ATCGGTGGCCGATGCGGCTGTCTTCGGTGTCCCGAACGAAGATCTCGGTG
AAGAGGTGAAGGCGGTCGTGCAGCTCATGCCTGGCGTCGCCGAATCCGAC
GAGACCGTGGCCGAATTGCTTCGGTTCTGCGGCGAACACCTCTCCCGCCA
GAAGGTGCCGCGCTCGATCGACTTCGAAGCCGAGCTGCCCAGACTGCCCA
CCGGCAAGCTCTACAAGCGCACCCTGCGAGACCGCTACTGGGGCGAAGGT
CAGAGCCGCATCCTCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig113193:112..579-

>mRNA_M-pyrifera_M_contig113193.2748.1 ID=mRNA_M-pyrifera_M_contig113193.2748.1|Name=mRNA_M-pyrifera_M_contig113193.2748.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=936bp|location=Sequence derived from alignment at M-pyrifera_M_contig113193:112..579- (Macrocystis pyrifera P11B4 male)
ACCGAGTTCGAGTACTTCAACGATCCGGCGAAGACGGCCGAAGCCACGTC
GGCCGACGGCACGATGACCAGCGTCGGCGATGTCGGGTATGTCGACGACG
ACGGGTTTCTCTACCTGACCGACCGGGCGACTTTCATGATCATCTCGGGC
GGAGTCAACATCTACCCGCAAGAGACCGAGAACCTCCTCATCACCCACCC
ATCGGTGGCCGATGCGGCTGTCTTCGGTGTCCCGAACGAAGATCTCGGTG
AAGAGGTGAAGGCGGTCGTGCAGCTCATGCCTGGCGTCGCCGAATCCGAC
GAGACCGTGGCCGAATTGCTTCGGTTCTGCGGCGAACACCTCTCCCGCCA
GAAGGTGCCGCGCTCGATCGACTTCGAAGCCGAGCTGCCCAGACTGCCCA
CCGGCAAGCTCTACAAGCGCACCCTGCGAGACCGCTACTGGGGCGAAGGT
CAGAGCCGCATCCTCTGAACCGAGTTCGAGTACTTCAACGATCCGGCGAA
GACGGCCGAAGCCACGTCGGCCGACGGCACGATGACCAGCGTCGGCGATG
TCGGGTATGTCGACGACGACGGGTTTCTCTACCTGACCGACCGGGCGACT
TTCATGATCATCTCGGGCGGAGTCAACATCTACCCGCAAGAGACCGAGAA
CCTCCTCATCACCCACCCATCGGTGGCCGATGCGGCTGTCTTCGGTGTCC
CGAACGAAGATCTCGGTGAAGAGGTGAAGGCGGTCGTGCAGCTCATGCCT
GGCGTCGCCGAATCCGACGAGACCGTGGCCGAATTGCTTCGGTTCTGCGG
CGAACACCTCTCCCGCCAGAAGGTGCCGCGCTCGATCGACTTCGAAGCCG
AGCTGCCCAGACTGCCCACCGGCAAGCTCTACAAGCGCACCCTGCGAGAC
CGCTACTGGGGCGAAGGTCAGAGCCGCATCCTCTGA
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