mRNA_M-pyrifera_M_contig111558.2405.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig111558.2405.1
Unique NamemRNA_M-pyrifera_M_contig111558.2405.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: D8LDL1_ECTSI (Lipase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDL1_ECTSI)

HSP 1 Score: 99.4 bits (246), Expect = 8.730e-24
Identity = 48/55 (87.27%), Postives = 51/55 (92.73%), Query Frame = 1 
Query:    1 MHQTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPR 165
            M+QTIK LY E GK+RKLYI GHSLGGALATIAAARL+FVDDMNIAGIYTIGSPR
Sbjct:  144 MYQTIKNLYNEHGKSRKLYIAGHSLGGALATIAAARLSFVDDMNIAGIYTIGSPR 198
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: D8LDK8_ECTSI (Putative lipase n=2 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDK8_ECTSI)

HSP 1 Score: 90.1 bits (222), Expect = 4.430e-20
Identity = 40/55 (72.73%), Postives = 50/55 (90.91%), Query Frame = 1 
Query:    1 MHQTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPR 165
            M + IK LY EKGK+RKLYI GHSLGGALAT+AAARL+++D+++IAG+YTIGSPR
Sbjct:  136 MRKVIKNLYNEKGKDRKLYIAGHSLGGALATVAAARLSYIDNLDIAGVYTIGSPR 190
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: A0A6H5JMK4_9PHAE (Lipase_3 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JMK4_9PHAE)

HSP 1 Score: 89.7 bits (221), Expect = 1.230e-19
Identity = 42/55 (76.36%), Postives = 48/55 (87.27%), Query Frame = 1 
Query:    1 MHQTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPR 165
            M+Q I  LY EKGKNRKL+  GHSLGGALAT AAAR+AF+DD++IAGIYTIGSPR
Sbjct:  233 MYQAIMDLYNEKGKNRKLFFAGHSLGGALATNAAARVAFIDDLDIAGIYTIGSPR 287
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: A0A6H5L4H8_9PHAE (Lipase_3 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L4H8_9PHAE)

HSP 1 Score: 87.8 bits (216), Expect = 4.340e-19
Identity = 41/55 (74.55%), Postives = 47/55 (85.45%), Query Frame = 1 
Query:    1 MHQTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPR 165
            M   IK  Y E+G++RKLYITGHSLGGALATIAAARL FVDD+N+A +YTIGSPR
Sbjct:  170 MLAVIKRFYHEEGRSRKLYITGHSLGGALATIAAARLVFVDDLNVAALYTIGSPR 224
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: A0A6H5KWV7_9PHAE (Lipase_3 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KWV7_9PHAE)

HSP 1 Score: 87.8 bits (216), Expect = 5.100e-19
Identity = 41/55 (74.55%), Postives = 49/55 (89.09%), Query Frame = 1 
Query:    1 MHQTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPR 165
            M   IKTL +E+GK+RKLY+ GHSLGGALAT+AAARLAF+DD+ I+GIYTIGSPR
Sbjct:  196 MRNIIKTLCKEEGKHRKLYLAGHSLGGALATVAAARLAFMDDVKISGIYTIGSPR 250
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: UPI0003793910 (DUF2974 domain-containing protein n=1 Tax=Nocardiopsis alba TaxID=53437 RepID=UPI0003793910)

HSP 1 Score: 61.6 bits (148), Expect = 7.960e-10
Identity = 28/51 (54.90%), Postives = 37/51 (72.55%), Query Frame = 1 
Query:   19 TLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPRYC 171
            TL E +  ++ +++TGHSLGGALA +AAARL F DD+   G+YT G PR C
Sbjct:  125 TLREFRDSDQTVWVTGHSLGGALAMLAAARLHFADDVLADGVYTFGQPRTC 175
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: J7LAN3_NOCAA (Lipase family protein n=2 Tax=Nocardiopsis alba TaxID=53437 RepID=J7LAN3_NOCAA)

HSP 1 Score: 61.6 bits (148), Expect = 1.070e-9
Identity = 28/51 (54.90%), Postives = 37/51 (72.55%), Query Frame = 1 
Query:   19 TLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPRYC 171
            TL E +  ++ +++TGHSLGGALA +AAARL F DD+   G+YT G PR C
Sbjct:  201 TLREFRDSDQTVWVTGHSLGGALAMLAAARLHFADDVLADGVYTFGQPRTC 251
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: UPI0016886C83 (Lipase family protein n=2 Tax=Leptolyngbya sp. FACHB-261 TaxID=2692806 RepID=UPI0016886C83)

HSP 1 Score: 60.5 bits (145), Expect = 2.600e-9
Identity = 30/53 (56.60%), Postives = 38/53 (71.70%), Query Frame = 1 
Query:    7 QTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPR 165
            + + TL   + KN+ L+ITGHSLGGALAT+A ARL F     +AG+YT GSPR
Sbjct:  152 RVMTTLDAFQDKNQSLWITGHSLGGALATLATARLRFGLKRPVAGLYTFGSPR 204
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: A0A223S1Y6_9ACTN (Lipase n=1 Tax=Nocardiopsis gilva YIM 90087 TaxID=1235441 RepID=A0A223S1Y6_9ACTN)

HSP 1 Score: 58.2 bits (139), Expect = 1.490e-8
Identity = 28/56 (50.00%), Postives = 37/56 (66.07%), Query Frame = 1 
Query:    7 QTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIA-GIYTIGSPRYC 171
            Q +  + E +G  + + ITGHSLGGALA +AAAR+ F D   +A G+YT G PR C
Sbjct:  121 QVLNAVSEMRGNGQSICITGHSLGGALAMLAAARMRFGDSRLLADGVYTFGQPRTC 176
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BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Match: A0A852TTT7_9ACTN (Pimeloyl-ACP methyl ester carboxylesterase n=1 Tax=Spinactinospora alkalitolerans TaxID=687207 RepID=A0A852TTT7_9ACTN)

HSP 1 Score: 57.8 bits (138), Expect = 2.040e-8
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:   13 IKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYTIGSPRYC 171
            +  + E +   + L+ TGHSLGGALA +AAAR+ FVD +   G+YT G PR C
Sbjct:  123 LDAVREFRADGQSLWFTGHSLGGALAMLAAARMHFVDGLLADGVYTFGQPRTC 175
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig111558.2405.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LDL1_ECTSI8.730e-2487.27Lipase n=1 Tax=Ectocarpus siliculosus TaxID=2880 R... [more]
D8LDK8_ECTSI4.430e-2072.73Putative lipase n=2 Tax=Ectocarpus siliculosus Tax... [more]
A0A6H5JMK4_9PHAE1.230e-1976.36Lipase_3 domain-containing protein n=2 Tax=Ectocar... [more]
A0A6H5L4H8_9PHAE4.340e-1974.55Lipase_3 domain-containing protein n=2 Tax=Ectocar... [more]
A0A6H5KWV7_9PHAE5.100e-1974.55Lipase_3 domain-containing protein n=2 Tax=Ectocar... [more]
UPI00037939107.960e-1054.90DUF2974 domain-containing protein n=1 Tax=Nocardio... [more]
J7LAN3_NOCAA1.070e-954.90Lipase family protein n=2 Tax=Nocardiopsis alba Ta... [more]
UPI0016886C832.600e-956.60Lipase family protein n=2 Tax=Leptolyngbya sp. FAC... [more]
A0A223S1Y6_9ACTN1.490e-850.00Lipase n=1 Tax=Nocardiopsis gilva YIM 90087 TaxID=... [more]
A0A852TTT7_9ACTN2.040e-849.06Pimeloyl-ACP methyl ester carboxylesterase n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig111558contigM-pyrifera_M_contig111558:316..486 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score100.1
Seed ortholog evalue3.4e-19
Seed eggNOG ortholog2880.D8LDL1
Hectar predicted targeting categoryno signal peptide or anchor
EggNOG free text desc.Lipase (class 3)
EggNOG OGs2EZH1@1,2T0TR@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size171
Cds size171
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815628.1171894-CDS-M-pyrifera_M_contig111558:315..4861622815628.1171894-CDS-M-pyrifera_M_contig111558:315..486Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig111558 316..486 -
1692277494.7531333-CDS-M-pyrifera_M_contig111558:315..4861692277494.7531333-CDS-M-pyrifera_M_contig111558:315..486Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig111558 316..486 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig111558.2405.1prot_M-pyrifera_M_contig111558.2405.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig111558 316..486 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig111558.2405.1

>prot_M-pyrifera_M_contig111558.2405.1 ID=prot_M-pyrifera_M_contig111558.2405.1|Name=mRNA_M-pyrifera_M_contig111558.2405.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=57bp
MHQTIKTLYEEKGKNRKLYITGHSLGGALATIAAARLAFVDDMNIAGIYT
IGSPRYC
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mRNA from alignment at M-pyrifera_M_contig111558:316..486-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig111558.2405.1 ID=mRNA_M-pyrifera_M_contig111558.2405.1|Name=mRNA_M-pyrifera_M_contig111558.2405.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=171bp|location=Sequence derived from alignment at M-pyrifera_M_contig111558:316..486- (Macrocystis pyrifera P11B4 male)
ATGCACCAAACCATCAAAACGCTCTACGAGGAGAAGGGGAAAAACAGGAA
GCTGTATATCACGGGACACTCCCTTGGAGGAGCCCTTGCCACCATCGCCG
CGGCCCGACTGGCCTTCGTGGATGACATGAACATCGCCGGGATCTACACT
ATCGGCAGCCCAAGGTACTGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig111558:316..486-

>mRNA_M-pyrifera_M_contig111558.2405.1 ID=mRNA_M-pyrifera_M_contig111558.2405.1|Name=mRNA_M-pyrifera_M_contig111558.2405.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=342bp|location=Sequence derived from alignment at M-pyrifera_M_contig111558:316..486- (Macrocystis pyrifera P11B4 male)
ATGCACCAAACCATCAAAACGCTCTACGAGGAGAAGGGGAAAAACAGGAA
GCTGTATATCACGGGACACTCCCTTGGAGGAGCCCTTGCCACCATCGCCG
CGGCCCGACTGGCCTTCGTGGATGACATGAACATCGCCGGGATCTACACT
ATCGGCAGCCCAAGGTACTGCATGCACCAAACCATCAAAACGCTCTACGA
GGAGAAGGGGAAAAACAGGAAGCTGTATATCACGGGACACTCCCTTGGAG
GAGCCCTTGCCACCATCGCCGCGGCCCGACTGGCCTTCGTGGATGACATG
AACATCGCCGGGATCTACACTATCGGCAGCCCAAGGTACTGC
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