mRNA_M-pyrifera_M_contig110622.2230.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig110622.2230.1
Unique NamemRNA_M-pyrifera_M_contig110622.2230.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: UPI001156B256 (beta-enolase n=1 Tax=Terrapene carolina triunguis TaxID=2587831 RepID=UPI001156B256)

HSP 1 Score: 217 bits (552), Expect = 1.350e-69
Identity = 107/134 (79.85%), Postives = 122/134 (91.04%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG ++   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGA+SF+EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+K+AVE+  +
Sbjct:   12 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNAEL--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKEAMRIGAEVYHNLKAVIKAKYGKDATNVGDEGGFAPNILENNEALELLKSAVEKAGY 143
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: UPI0004A340FE (beta-enolase-like n=1 Tax=Poecilia reticulata TaxID=8081 RepID=UPI0004A340FE)

HSP 1 Score: 219 bits (558), Expect = 4.470e-69
Identity = 108/134 (80.60%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGAS+F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+KTA+E+  +
Sbjct:   12 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGASNFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGY 143
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A674JYW3_TERCA (Phosphopyruvate hydratase n=1 Tax=Terrapene carolina triunguis TaxID=2587831 RepID=A0A674JYW3_TERCA)

HSP 1 Score: 217 bits (552), Expect = 9.610e-69
Identity = 107/134 (79.85%), Postives = 122/134 (91.04%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG ++   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGA+SF+EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+K+AVE+  +
Sbjct:   89 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNAEL--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKEAMRIGAEVYHNLKAVIKAKYGKDATNVGDEGGFAPNILENNEALELLKSAVEKAGY 220
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A061ADL0_ONCMY (Phosphopyruvate hydratase n=1 Tax=Oncorhynchus mykiss TaxID=8022 RepID=A0A061ADL0_ONCMY)

HSP 1 Score: 215 bits (548), Expect = 1.300e-68
Identity = 105/134 (78.36%), Postives = 120/134 (89.55%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++P P+FNVINGGSHAGNKLAMQEFMILP+GAS+F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+K+A+E+  +
Sbjct:   12 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPCPAFNVINGGSHAGNKLAMQEFMILPIGASNFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKSAIEKAGY 143
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: M4AKL6_XIPMA (Phosphopyruvate hydratase n=1 Tax=Xiphophorus maculatus TaxID=8083 RepID=M4AKL6_XIPMA)

HSP 1 Score: 219 bits (558), Expect = 2.040e-68
Identity = 108/134 (80.60%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGAS+F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+KTA+E+  +
Sbjct:   40 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGASNFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGY 171
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A2D4NL86_MICSU (Phosphopyruvate hydratase (Fragment) n=1 Tax=Micrurus surinamensis TaxID=129470 RepID=A0A2D4NL86_MICSU)

HSP 1 Score: 217 bits (552), Expect = 2.320e-68
Identity = 106/134 (79.10%), Postives = 122/134 (91.04%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG  +K  ++PVP+FNVINGGSHAGNKLAMQEFMILPVGA+SF+EAMRIGAE+Y NLK VIK+KYG+DA NVGDEGGFAPNILEN E LEL+K+A+E+  +
Sbjct:  101 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAG--NKELILPVPAFNVINGGSHAGNKLAMQEFMILPVGATSFKEAMRIGAEVYHNLKAVIKSKYGKDATNVGDEGGFAPNILENNEALELLKSAIEKAGY 232
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A3Q2PRP1_FUNHE (Phosphopyruvate hydratase n=1 Tax=Fundulus heteroclitus TaxID=8078 RepID=A0A3Q2PRP1_FUNHE)

HSP 1 Score: 219 bits (558), Expect = 2.410e-68
Identity = 108/134 (80.60%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGAS+F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+KTA+E+  +
Sbjct:   74 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGASNFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGY 205
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BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A665TK18_ECHNA (Phosphopyruvate hydratase n=1 Tax=Echeneis naucrates TaxID=173247 RepID=A0A665TK18_ECHNA)

HSP 1 Score: 218 bits (555), Expect = 2.870e-68
Identity = 107/134 (79.85%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGA++F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+KTA+E+  +
Sbjct:   12 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGY 143
[Close]
BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A3Q2D3I7_CYPVA (Phosphopyruvate hydratase n=2 Tax=Percomorphaceae TaxID=1489872 RepID=A0A3Q2D3I7_CYPVA)

HSP 1 Score: 219 bits (558), Expect = 3.730e-68
Identity = 108/134 (80.60%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGAS+F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+KTA+E+  +
Sbjct:   62 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGASNFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGY 193
[Close]
BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Match: A0A3P8V549_CYNSE (Phosphopyruvate hydratase n=3 Tax=Percomorphaceae TaxID=1489872 RepID=A0A3P8V549_CYNSE)

HSP 1 Score: 219 bits (558), Expect = 3.730e-68
Identity = 108/134 (80.60%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQDACNVGDEGGFAPNILENREGLELIKTAVEEGAH 402
            K GANAILGVSLAVCKAGAAEKGVPL+RHI+DLAG+ D   ++PVP+FNVINGGSHAGNKLAMQEFMILPVGAS+F EAMRIGAE+Y NLK VIKAKYG+DA NVGDEGGFAPNILEN E LEL+KTA+E+  +
Sbjct:   62 KFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDV--ILPVPAFNVINGGSHAGNKLAMQEFMILPVGASNFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGY 193
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig110622.2230.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001156B2561.350e-6979.85beta-enolase n=1 Tax=Terrapene carolina triunguis ... [more]
UPI0004A340FE4.470e-6980.60beta-enolase-like n=1 Tax=Poecilia reticulata TaxI... [more]
A0A674JYW3_TERCA9.610e-6979.85Phosphopyruvate hydratase n=1 Tax=Terrapene caroli... [more]
A0A061ADL0_ONCMY1.300e-6878.36Phosphopyruvate hydratase n=1 Tax=Oncorhynchus myk... [more]
M4AKL6_XIPMA2.040e-6880.60Phosphopyruvate hydratase n=1 Tax=Xiphophorus macu... [more]
A0A2D4NL86_MICSU2.320e-6879.10Phosphopyruvate hydratase (Fragment) n=1 Tax=Micru... [more]
A0A3Q2PRP1_FUNHE2.410e-6880.60Phosphopyruvate hydratase n=1 Tax=Fundulus heteroc... [more]
A0A665TK18_ECHNA2.870e-6879.85Phosphopyruvate hydratase n=1 Tax=Echeneis naucrat... [more]
A0A3Q2D3I7_CYPVA3.730e-6880.60Phosphopyruvate hydratase n=2 Tax=Percomorphaceae ... [more]
A0A3P8V549_CYNSE3.730e-6880.60Phosphopyruvate hydratase n=3 Tax=Percomorphaceae ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig110622contigM-pyrifera_M_contig110622:7..408 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score220.7
Seed ortholog evalue4.1e-55
Seed eggNOG ortholog106582.XP_004573823.1
Preferred nameENO3
KEGG rclassRC00349
KEGG koko:K01689
KEGG ReactionR00658
KEGG Pathwayko00010,ko00680,ko01100,ko01110,ko01120,ko01130,ko01200,ko01230,ko03018,ko04066,map00010,map00680,map01100,map01110,map01120,map01130,map01200,map01230,map03018,map04066
KEGG ModuleM00001,M00002,M00003,M00346,M00394
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0003824,GO:0004634,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005886,GO:0005975,GO:0005996,GO:0006006,GO:0006007,GO:0006082,GO:0006090,GO:0006091,GO:0006094,GO:0006096,GO:0006139,GO:0006163,GO:0006164,GO:0006165,GO:0006725,GO:0006732,GO:0006733,GO:0006734,GO:0006735,GO:0006753,GO:0006754,GO:0006757,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0007568,GO:0008150,GO:0008152,GO:0009056,GO:0009058,GO:0009108,GO:0009117,GO:0009123,GO:0009124,GO:0009126,GO:0009127,GO:0009132,GO:0009135,GO:0009141,GO:0009142,GO:0009144,GO:0009145,GO:0009150,GO:0009152,GO:0009156,GO:0009161,GO:0009165,GO:0009166,GO:0009167,GO:0009168,GO:0009179,GO:0009185,GO:0009199,GO:0009201,GO:0009205,GO:0009206,GO:0009259,GO:0009260,GO:0009435,GO:0009611,GO:0009888,GO:0009987,GO:0016020,GO:0016051,GO:0016052,GO:0016053,GO:0016310,GO:0016829,GO:0016835,GO:0016836,GO:0017144,GO:0018130,GO:0019318,GO:0019319,GO:0019320,GO:0019359,GO:0019362,GO:0019363,GO:0019438,GO:0019439,GO:0019637,GO:0019674,GO:0019693,GO:0019752,GO:0031099,GO:0032502,GO:0032787,GO:0034404,GO:0034641,GO:0034654,GO:0034655,GO:0040007,GO:0042060,GO:0042221,GO:0042246,GO:0042493,GO:0042802,GO:0042803,GO:0042866,GO:0043403,GO:0043436,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044270,GO:0044271,GO:0044281,GO:0044282,GO:0044283,GO:0044424,GO:0044444,GO:0044464,GO:0046031,GO:0046034,GO:0046364,GO:0046365,GO:0046390,GO:0046394,GO:0046434,GO:0046483,GO:0046496,GO:0046700,GO:0046939,GO:0046982,GO:0046983,GO:0048589,GO:0048856,GO:0050896,GO:0051186,GO:0051188,GO:0055086,GO:0055114,GO:0061615,GO:0061620,GO:0061621,GO:0061718,GO:0071704,GO:0071944,GO:0072330,GO:0072521,GO:0072522,GO:0072524,GO:0072525,GO:0090407,GO:1901135,GO:1901137,GO:1901292,GO:1901293,GO:1901360,GO:1901361,GO:1901362,GO:1901564,GO:1901566,GO:1901575,GO:1901576
EggNOG free text desc.Enolase 3, (beta, muscle)
EggNOG OGs38C66@33154,3BE2S@33208,3CU4G@33213,480BN@7711,4928M@7742,49S9W@7898,COG0148@1,KOG2670@2759
EC4.2.1.11
COG Functional cat.G
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03019,ko04147
Exons1
Model size402
Cds size402
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815618.0243917-CDS-M-pyrifera_M_contig110622:6..4081622815618.0243917-CDS-M-pyrifera_M_contig110622:6..408Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110622 7..408 -
1692277489.9266725-CDS-M-pyrifera_M_contig110622:6..4081692277489.9266725-CDS-M-pyrifera_M_contig110622:6..408Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110622 7..408 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig110622.2230.1prot_M-pyrifera_M_contig110622.2230.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig110622 7..408 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig110622.2230.1

>prot_M-pyrifera_M_contig110622.2230.1 ID=prot_M-pyrifera_M_contig110622.2230.1|Name=mRNA_M-pyrifera_M_contig110622.2230.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=134bp
KLGANAILGVSLAVCKAGAAEKGVPLFRHISDLAGYSDKPWVMPVPSFNV
INGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEIYQNLKLVIKAKYGQ
DACNVGDEGGFAPNILENREGLELIKTAVEEGAH
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mRNA from alignment at M-pyrifera_M_contig110622:7..408-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig110622.2230.1 ID=mRNA_M-pyrifera_M_contig110622.2230.1|Name=mRNA_M-pyrifera_M_contig110622.2230.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=402bp|location=Sequence derived from alignment at M-pyrifera_M_contig110622:7..408- (Macrocystis pyrifera P11B4 male)
AAGCTGGGCGCCAACGCAATCCTGGGCGTGTCGCTTGCTGTGTGCAAGGC
GGGGGCTGCGGAGAAGGGCGTGCCGCTCTTCAGGCACATCTCGGACTTGG
CTGGCTACAGCGACAAGCCCTGGGTGATGCCCGTGCCCTCGTTCAACGTC
ATCAACGGCGGCAGCCACGCAGGCAACAAGCTGGCGATGCAGGAGTTCAT
GATCCTGCCGGTGGGCGCCTCCTCCTTCCGCGAGGCAATGCGCATTGGCG
CAGAGATCTACCAGAACCTCAAGCTCGTCATCAAGGCTAAGTACGGCCAG
GACGCTTGCAACGTCGGCGACGAGGGCGGCTTCGCCCCCAACATCCTCGA
GAACCGCGAGGGCCTCGAGCTCATCAAGACCGCCGTCGAGGAGGGTGCGC
AC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig110622:7..408-

>mRNA_M-pyrifera_M_contig110622.2230.1 ID=mRNA_M-pyrifera_M_contig110622.2230.1|Name=mRNA_M-pyrifera_M_contig110622.2230.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=804bp|location=Sequence derived from alignment at M-pyrifera_M_contig110622:7..408- (Macrocystis pyrifera P11B4 male)
AAGCTGGGCGCCAACGCAATCCTGGGCGTGTCGCTTGCTGTGTGCAAGGC
GGGGGCTGCGGAGAAGGGCGTGCCGCTCTTCAGGCACATCTCGGACTTGG
CTGGCTACAGCGACAAGCCCTGGGTGATGCCCGTGCCCTCGTTCAACGTC
ATCAACGGCGGCAGCCACGCAGGCAACAAGCTGGCGATGCAGGAGTTCAT
GATCCTGCCGGTGGGCGCCTCCTCCTTCCGCGAGGCAATGCGCATTGGCG
CAGAGATCTACCAGAACCTCAAGCTCGTCATCAAGGCTAAGTACGGCCAG
GACGCTTGCAACGTCGGCGACGAGGGCGGCTTCGCCCCCAACATCCTCGA
GAACCGCGAGGGCCTCGAGCTCATCAAGACCGCCGTCGAGGAGGGTGCGC
ACAAGCTGGGCGCCAACGCAATCCTGGGCGTGTCGCTTGCTGTGTGCAAG
GCGGGGGCTGCGGAGAAGGGCGTGCCGCTCTTCAGGCACATCTCGGACTT
GGCTGGCTACAGCGACAAGCCCTGGGTGATGCCCGTGCCCTCGTTCAACG
TCATCAACGGCGGCAGCCACGCAGGCAACAAGCTGGCGATGCAGGAGTTC
ATGATCCTGCCGGTGGGCGCCTCCTCCTTCCGCGAGGCAATGCGCATTGG
CGCAGAGATCTACCAGAACCTCAAGCTCGTCATCAAGGCTAAGTACGGCC
AGGACGCTTGCAACGTCGGCGACGAGGGCGGCTTCGCCCCCAACATCCTC
GAGAACCGCGAGGGCCTCGAGCTCATCAAGACCGCCGTCGAGGAGGGTGC
GCAC
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