mRNA_M-pyrifera_M_contig105449.1164.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig105449.1164.1
Unique NamemRNA_M-pyrifera_M_contig105449.1164.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: L8GCN9_ACACA (TRRAP family protein (Fragment) n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GCN9_ACACA)

HSP 1 Score: 182 bits (461), Expect = 1.190e-49
Identity = 99/158 (62.66%), Postives = 118/158 (74.68%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSLKHSIPELLEASKQKDEDDKEAGVNWQSDRSNDQ------VKDCRLLLKTLVLGLKNIVWGISSC 456
            TS ETLRPLAYSTL DLVHHVR DL+L QLSRVV L++ NIHD TLPF IQTMSAK+LLNLVE I RK S+  G+GR +LV ILD FVNKFSSLK  IP+++  S+  ++DD + G      R   Q      +KDCR+L+K LV+GLKNIVWGI+SC
Sbjct:  362 TSFETLRPLAYSTLADLVHHVRTDLSLPQLSRVVQLFARNIHDSTLPFNIQTMSAKLLLNLVEGIARKTSDVEGKGRALLVRILDNFVNKFSSLKKQIPKIV--SQPLEDDDAKPGTLQSQKRKRSQARTLASIKDCRVLMKNLVMGLKNIVWGITSC 517          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A2P6N7T1_9EUKA (FAT domain-containing protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6N7T1_9EUKA)

HSP 1 Score: 150 bits (380), Expect = 9.390e-39
Identity = 79/165 (47.88%), Postives = 114/165 (69.09%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKN-SESNGR-----------GRPVLVHILDAFVNKFSSLKHSIPELLEASKQKDEDDKEAGVNWQSDRSNDQVKDCRLLLKTLVLGLKNIVWGISSCN 459
            T+ +TLRPLA+STL DL+HH+R DLT  QL RVV+LY+ N+HD TL + +QTMS K+++NLVE IV++N +E  G            GR +L+ IL+  VNKFSSL+  IP+++E +K K              + ND+++DCR+L++TLV+ LKNI+WGIS+C+
Sbjct:  343 TAFDTLRPLAFSTLADLIHHIRNDLTWPQLQRVVYLYTRNLHDSTLIYTVQTMSVKLIINLVEGIVKRNDAEGKGEAMQQVERFTLTGRTLLIRILEVAVNKFSSLRKEIPKVIEKAKNKK-------------KGNDKIRDCRILMRTLVVSLKNIMWGISNCS 494          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A6L2PW01_COPFO (Non-specific serine/threonine protein kinase n=1 Tax=Coptotermes formosanus TaxID=36987 RepID=A0A6L2PW01_COPFO)

HSP 1 Score: 142 bits (359), Expect = 6.220e-36
Identity = 80/155 (51.61%), Postives = 102/155 (65.81%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSL-KHSIPELLEASKQKDE--DDKEAGVNWQSDRSNDQVKDCRLLLKTLVLGLKNIVWGISSC 456
            T+HE+LRPLAYSTL DLVHHVR  L    L+R VHL++ N+HD TLP  IQTMS K+LLNLVECI  ++ E  G+GR +L+ +L+ FV KF ++ K  +P L+     +D    DK       S  SN  V DCR L+KTLV G+K I WG +SC
Sbjct:  384 TTHESLRPLAYSTLADLVHHVRQHLPFSDLARAVHLFAKNVHDETLPTSIQTMSCKLLLNLVECIRTRSEEEKGQGRELLMRMLEVFVLKFKTIAKLQLPVLMSKWYVQDSLIKDKSRFGFPSSQASNYSVTDCRGLVKTLVCGVKTITWGCASC 538          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A7S4DVY0_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S4DVY0_9EUKA)

HSP 1 Score: 139 bits (350), Expect = 1.770e-35
Identity = 81/174 (46.55%), Postives = 111/174 (63.79%), Query Frame = 1
Query:    4 SHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSLKHSIPELLEASKQKDEDDKEAGVNW--------QSDRSNDQVKDCRLLLKTLVLGLKNIVWGISSCNSKFSSVPSSGPSAA 501
            +HE +RPLAYSTL DLVHHVRADL++ QLS+V++++S NIHDPTLP  IQT S +++L LV+ I R  S+   RGR +LV IL A VNKF +LK  IP+L++   +K +  +  G  W        + +   D V++ + L+ T+V GLK +VW    C S F S   SG  AA
Sbjct:  200 AHEIVRPLAYSTLADLVHHVRADLSIDQLSKVIYIFSRNIHDPTLPLSIQTASVRLILTLVDHIYR-TSDKQDRGRTLLVRILSALVNKFDTLKDYIPKLIDLVNKKKKRVQAKGHAWTLAYTPRSEKEFPEDSVREVKQLMFTMVFGLKTVVW----CISNFRSHSQSGTHAA 368          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A6P5AYC1_BRABE (transformation/transcription domain-associated protein-like n=2 Tax=Branchiostoma TaxID=7737 RepID=A0A6P5AYC1_BRABE)

HSP 1 Score: 139 bits (349), Expect = 1.360e-34
Identity = 77/153 (50.33%), Postives = 99/153 (64.71%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSL-KHSIPELLEASKQKDEDDKEAGVNWQSDRSNDQVKDCRLLLKTLVLGLKNIVWGISSC 456
            T+ ETLRPLAYSTL DLVHHVR  L L  L+  VHL+S N+HD  LP  IQTMS K+LLNLVECI  K+ +  G GR +L+ +L+ FV KF ++ K  +P +     ++ E++K      Q   +N  V DCR L+KTLV G+K I WG  SC
Sbjct:  367 TTRETLRPLAYSTLADLVHHVRQHLQLSDLALAVHLFSKNVHDDLLPSSIQTMSCKLLLNLVECI--KSDQDGGNGREILMRMLEVFVLKFHTIAKFQLPGIFAKCDKEKEEEKSKPAGGQF--NNFSVSDCRSLVKTLVCGVKTITWGAQSC 515          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A2K1J4Q0_PHYPA (Uncharacterized protein n=6 Tax=Physcomitrium patens TaxID=3218 RepID=A0A2K1J4Q0_PHYPA)

HSP 1 Score: 139 bits (349), Expect = 1.370e-34
Identity = 80/167 (47.90%), Postives = 109/167 (65.27%), Query Frame = 1
Query:    7 HETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSE--SNGRGRPVLVHILDAFVNKFSSLKHSIPELLEASKQKDEDDKE-------------AGVNWQ-SDRSNDQVKDCRLLLKTLVLGLKNIVWGISSCN 459
            +ETLRPLAYS L +L+HHVR DL+L QLSR+V+L+S N+HD +LP  +QT   +++LNLVE I  +  +  S   GR +L  ILDAFV+KF +LKHSIP+LLE   +  ED KE             A +N Q     + +V DC+ LLKTL++G+K +VW I+  N
Sbjct:  342 YETLRPLAYSLLAELIHHVRLDLSLPQLSRIVYLFSRNVHDSSLPLSVQTTCVRLMLNLVETIFGRRVDHLSREEGRALLGRILDAFVSKFGTLKHSIPQLLE---EDGEDRKERRTLRSRLELPVQAALNLQVPPEHSKEVMDCKQLLKTLIMGMKTLVWSITHFN 505          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A2R6XKQ8_MARPO (Uncharacterized protein n=3 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6XKQ8_MARPO)

HSP 1 Score: 137 bits (346), Expect = 3.470e-34
Identity = 80/178 (44.94%), Postives = 108/178 (60.67%), Query Frame = 1
Query:    7 HETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIV--RKNSESNGRGRPVLVHILDAFVNKFSSLKHSIPELLEASKQKDEDDKEAGVNWQSDRS------------------NDQVKDCRLLLKTLVLGLKNIVWGISSCNSKFSSVP 480
            +ETLRPLAYS L +L+HHVR DL+L QLSR+V+L+S N+HD +LP  +QT   +++LNLVE I   R +  S   GR +L  ILDAFV+KF +LKH IP+LLE        D E G    S RS                  + +V DC+ L+KTL++G+K +VW I+  N+    VP
Sbjct:  341 YETLRPLAYSLLAELIHHVRLDLSLPQLSRIVYLFSRNVHDSSLPLSVQTTCVRLMLNLVETIFSRRVDQHSKEEGRALLGRILDAFVSKFGTLKHMIPQLLEGG------DAEDGKERPSLRSRLELPVQSALNLPVPPEHSKEVSDCKQLIKTLIMGMKTLVWSITHFNATQMQVP 512          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: UPI0018962F72 (LOW QUALITY PROTEIN: transformation/transcription domain-associated protein-like n=1 Tax=Rhipicephalus sanguineus TaxID=34632 RepID=UPI0018962F72)

HSP 1 Score: 136 bits (343), Expect = 8.760e-34
Identity = 77/169 (45.56%), Postives = 109/169 (64.50%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSL-KHSIPELLEASKQ--------KDEDDKEAGVNWQS-------DRSNDQ-VKDCRLLLKTLVLGLKNIVWGISSC 456
            T++E+LRPLAYSTL DLVHHVR  L L  LS  V ++S N+HD +LP  IQTMS K+LLNLVECI  ++ + NG GR +L+ +L+ FV KF ++ K  +P L++ S+Q        + +D   A + + +       +++N   V DCR L+KTLV G+K I WGI +C
Sbjct:  379 TAYESLRPLAYSTLADLVHHVRQHLPLNNLSSAVAVFSRNVHDESLPASIQTMSCKLLLNLVECIRTRSDQENGNGRELLIRMLEVFVLKFKTIAKCQLPVLMQKSRQQGPPSEANRPDDSARASLQFSASLADLKEEKNNSYTVADCRSLVKTLVCGVKTITWGIGTC 547          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: A0A224YZY6_9ACAR (Histone acetyltransferase saga trrap/tra1 component pi 3 kinase superfamily n=4 Tax=Rhipicephalinae TaxID=426437 RepID=A0A224YZY6_9ACAR)

HSP 1 Score: 136 bits (343), Expect = 8.760e-34
Identity = 77/169 (45.56%), Postives = 109/169 (64.50%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSL-KHSIPELLEASKQ--------KDEDDKEAGVNWQS-------DRSNDQ-VKDCRLLLKTLVLGLKNIVWGISSC 456
            T++E+LRPLAYSTL DLVHHVR  L L  LS  V ++S N+HD +LP  IQTMS K+LLNLVECI  ++ + NG GR +L+ +L+ FV KF ++ K  +P L++ S+Q        + +D   A + + +       +++N   V DCR L+KTLV G+K I WGI +C
Sbjct:  375 TAYESLRPLAYSTLADLVHHVRQHLPLNNLSSAVAVFSRNVHDESLPASIQTMSCKLLLNLVECIRTRSDQENGNGRELLIRMLEVFVLKFKTIAKCQLPVLMQKSRQQGPPSEANRPDDSARASLQFSASLADLKEEKNNSYTVADCRSLVKTLVCGVKTITWGIGTC 543          
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Match: I3KUZ2_ORENI (Uncharacterized protein n=89 Tax=Clupeocephala TaxID=186625 RepID=I3KUZ2_ORENI)

HSP 1 Score: 136 bits (342), Expect = 1.190e-33
Identity = 76/153 (49.67%), Postives = 102/153 (66.67%), Query Frame = 1
Query:    1 TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGIQTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSL-KHSIPELLEASKQKDEDDKEAGVNWQSDRSNDQVKDCRLLLKTLVLGLKNIVWGISSC 456
            T+ ETLRPLAYSTL DLVHHVR +LTL  LS  V L++ NI D +LP  IQTMS K+LLNLV+CI  K+ + NG GR +L+ +L+ FV KF ++ ++ +  + +  K + E      V+  +     QV DCR L+KTLV G+K I WGI+SC
Sbjct:  366 TARETLRPLAYSTLADLVHHVRQNLTLTDLSLAVQLFAKNIDDESLPSNIQTMSCKLLLNLVDCIRSKSEQENGNGRDILMRMLEVFVLKFHTIARYQLVSIFKKCKPQSE---MGVVDPGAIPGTFQVSDCRSLVKTLVCGVKTITWGITSC 515          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig105449.1164.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
L8GCN9_ACACA1.190e-4962.66TRRAP family protein (Fragment) n=1 Tax=Acanthamoe... [more]
A0A2P6N7T1_9EUKA9.390e-3947.88FAT domain-containing protein n=1 Tax=Planoprotost... [more]
A0A6L2PW01_COPFO6.220e-3651.61Non-specific serine/threonine protein kinase n=1 T... [more]
A0A7S4DVY0_9EUKA1.770e-3546.55Hypothetical protein (Fragment) n=1 Tax=Lotharella... [more]
A0A6P5AYC1_BRABE1.360e-3450.33transformation/transcription domain-associated pro... [more]
A0A2K1J4Q0_PHYPA1.370e-3447.90Uncharacterized protein n=6 Tax=Physcomitrium pate... [more]
A0A2R6XKQ8_MARPO3.470e-3444.94Uncharacterized protein n=3 Tax=Marchantia polymor... [more]
UPI0018962F728.760e-3445.56LOW QUALITY PROTEIN: transformation/transcription ... [more]
A0A224YZY6_9ACAR8.760e-3445.56Histone acetyltransferase saga trrap/tra1 componen... [more]
I3KUZ2_ORENI1.190e-3349.67Uncharacterized protein n=89 Tax=Clupeocephala Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig105449contigM-pyrifera_M_contig105449:2..562 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score140.2
Seed ortholog evalue9.8e-31
Seed eggNOG ortholog3218.PP1S65_237V6.1
Preferred nameTRRAP
KEGG koko:K08852,ko:K08874
KEGG Pathwayko04140,ko04141,ko04210,ko04932,ko05010,ko05166,map04140,map04141,map04210,map04932,map05010,map05166
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Belongs to the PI3 PI4-kinase family
EggNOG OGs37KZ4@33090,3GEXY@35493,COG5032@1,KOG0889@2759
EC2.7.11.1
COG Functional cat.BDLT
Best tax levelStreptophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001,ko03021,ko03036
Exons1
Model size561
Cds size561
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815562.511544-CDS-M-pyrifera_M_contig105449:1..5621622815562.511544-CDS-M-pyrifera_M_contig105449:1..562Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig105449 2..562 +
1692277465.1447031-CDS-M-pyrifera_M_contig105449:1..5621692277465.1447031-CDS-M-pyrifera_M_contig105449:1..562Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig105449 2..562 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig105449.1164.1prot_M-pyrifera_M_contig105449.1164.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig105449 2..562 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig105449.1164.1

>prot_M-pyrifera_M_contig105449.1164.1 ID=prot_M-pyrifera_M_contig105449.1164.1|Name=mRNA_M-pyrifera_M_contig105449.1164.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=187bp
TSHETLRPLAYSTLVDLVHHVRADLTLQQLSRVVHLYSCNIHDPTLPFGI
QTMSAKILLNLVECIVRKNSESNGRGRPVLVHILDAFVNKFSSLKHSIPE
LLEASKQKDEDDKEAGVNWQSDRSNDQVKDCRLLLKTLVLGLKNIVWGIS
SCNSKFSSVPSSGPSAASVAAAAGPTSADYAAALAPA
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mRNA from alignment at M-pyrifera_M_contig105449:2..562+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig105449.1164.1 ID=mRNA_M-pyrifera_M_contig105449.1164.1|Name=mRNA_M-pyrifera_M_contig105449.1164.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=561bp|location=Sequence derived from alignment at M-pyrifera_M_contig105449:2..562+ (Macrocystis pyrifera P11B4 male)
ACGTCGCATGAGACTCTTCGCCCTCTCGCGTACAGCACACTTGTGGACCT GGTCCATCACGTGCGCGCAGACCTGACTCTCCAACAGCTGTCCAGAGTCG TACACCTCTACTCTTGCAACATCCACGACCCCACCTTGCCGTTTGGGATC CAAACCATGTCTGCCAAGATCCTGCTCAACTTGGTGGAGTGCATCGTCAG GAAGAACTCGGAGTCGAACGGGCGAGGCAGGCCTGTGCTCGTTCACATCC TGGACGCCTTCGTCAACAAGTTCAGCAGCCTCAAGCACAGCATTCCCGAG CTTTTGGAGGCATCCAAGCAAAAGGATGAGGATGACAAGGAGGCAGGAGT CAACTGGCAGAGCGACAGGTCGAACGATCAAGTGAAGGACTGCCGTCTCC TGTTGAAGACGCTCGTACTCGGCCTGAAGAACATTGTGTGGGGCATCAGC TCATGCAACTCCAAATTCAGTTCAGTGCCCTCCTCGGGCCCCTCGGCTGC CTCCGTGGCTGCGGCTGCAGGTCCGACCAGCGCCGACTATGCTGCAGCTC TGGCCCCGGCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig105449:2..562+

>mRNA_M-pyrifera_M_contig105449.1164.1 ID=mRNA_M-pyrifera_M_contig105449.1164.1|Name=mRNA_M-pyrifera_M_contig105449.1164.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1122bp|location=Sequence derived from alignment at M-pyrifera_M_contig105449:2..562+ (Macrocystis pyrifera P11B4 male)
ACGTCGCATGAGACTCTTCGCCCTCTCGCGTACAGCACACTTGTGGACCT
GGTCCATCACGTGCGCGCAGACCTGACTCTCCAACAGCTGTCCAGAGTCG
TACACCTCTACTCTTGCAACATCCACGACCCCACCTTGCCGTTTGGGATC
CAAACCATGTCTGCCAAGATCCTGCTCAACTTGGTGGAGTGCATCGTCAG
GAAGAACTCGGAGTCGAACGGGCGAGGCAGGCCTGTGCTCGTTCACATCC
TGGACGCCTTCGTCAACAAGTTCAGCAGCCTCAAGCACAGCATTCCCGAG
CTTTTGGAGGCATCCAAGCAAAAGGATGAGGATGACAAGGAGGCAGGAGT
CAACTGGCAGAGCGACAGGTCGAACGATCAAGTGAAGGACTGCCGTCTCC
TGTTGAAGACGCTCGTACTCGGCCTGAAGAACATTGTGTGGGGCATCAGC
TCATGCAACTCCAAATTCAGTTCAGTGCCCTCCTCGGGCCCCTCGGCTGC
CTCCGTGGCTGCGGCTGCAGGTCCGACCAGCGCCGACTATGCTGCAGCTC
TGGCCCCGGCTACGTCGCATGAGACTCTTCGCCCTCTCGCGTACAGCACA
CTTGTGGACCTGGTCCATCACGTGCGCGCAGACCTGACTCTCCAACAGCT
GTCCAGAGTCGTACACCTCTACTCTTGCAACATCCACGACCCCACCTTGC
CGTTTGGGATCCAAACCATGTCTGCCAAGATCCTGCTCAACTTGGTGGAG
TGCATCGTCAGGAAGAACTCGGAGTCGAACGGGCGAGGCAGGCCTGTGCT
CGTTCACATCCTGGACGCCTTCGTCAACAAGTTCAGCAGCCTCAAGCACA
GCATTCCCGAGCTTTTGGAGGCATCCAAGCAAAAGGATGAGGATGACAAG
GAGGCAGGAGTCAACTGGCAGAGCGACAGGTCGAACGATCAAGTGAAGGA
CTGCCGTCTCCTGTTGAAGACGCTCGTACTCGGCCTGAAGAACATTGTGT
GGGGCATCAGCTCATGCAACTCCAAATTCAGTTCAGTGCCCTCCTCGGGC
CCCTCGGCTGCCTCCGTGGCTGCGGCTGCAGGTCCGACCAGCGCCGACTA
TGCTGCAGCTCTGGCCCCGGCT
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