mRNA_M-pyrifera_M_contig104467.962.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig104467.962.1
Unique NamemRNA_M-pyrifera_M_contig104467.962.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A7Y5SMK9_9BACT (LamGL domain-containing protein n=1 Tax=Pirellulales bacterium TaxID=2715253 RepID=A0A7Y5SMK9_9BACT)

HSP 1 Score: 160 bits (404), Expect = 2.290e-43
Identity = 79/128 (61.72%), Postives = 96/128 (75.00%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGT-SHPGRLVYLH----DG-ETVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLS 366
            +FSRGRDHGL   GDHLG+GGT S PG+L++LH    DG + VAG + +ERWTW+HVVFV DG  VRIYL+G +DPEI  TS+   P   ++  FGGR D  S+WEGRLDEIAVFDRALSA+E+  LS
Sbjct:  582 LFSRGRDHGLDPHGDHLGLGGTASQPGKLIFLHGDPKDGAKPVAGSTAIERWTWNHVVFVHDGDTVRIYLNGKADPEIQATSAAGFPPGFDQWFFGGRCDNDSNWEGRLDEIAVFDRALSAKEIAKLS 709          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A7C7UZM2_9PLAN (LamG domain-containing protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A7C7UZM2_9PLAN)

HSP 1 Score: 148 bits (373), Expect = 4.230e-42
Identity = 69/128 (53.91%), Postives = 91/128 (71.09%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTS-HPGRLVYLHD-----GETVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLS 366
            + SR RDHGL   GDH+G+GGT+  PGR+++LH      G T  G++ + RW+W+H+VFVRDG  VRIYL+G  +PEI + S    P    +L FGGR D QS+WEGRLDEIAVFDRAL+ E+V+ L+
Sbjct:  117 ILSRERDHGLGPWGDHVGLGGTATKPGRIIFLHGKPSGHGVTAVGRTDIPRWSWNHLVFVRDGDRVRIYLNGNPEPEIDVASKADFPPGFGQLFFGGRSDNQSNWEGRLDEIAVFDRALTGEDVQKLN 244          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A517VW69_9PLAN (Neutral/alkaline non-lysosomal ceramidase n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517VW69_9PLAN)

HSP 1 Score: 150 bits (380), Expect = 5.030e-40
Identity = 72/125 (57.60%), Postives = 94/125 (75.20%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTSHPGRLVYLH--DGETVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTP-TAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLS 366
            MFS+G +HGL + GD LGVGGT HPG+LVY++  D +  AG++ +ERWTW+HV  VRDG+ VR+YL+G + PEI + S   T    +E+  FGGR D QS+WEGRLDEIAVFDR L+AEE++ LS
Sbjct:  584 MFSQGPNHGLAAYGDFLGVGGTQHPGKLVYMNGADRKLHAGKTAIERWTWNHVALVRDGKMVRVYLNGNTKPEIEMESVPGTEGVVLEQFFFGGRTDNQSNWEGRLDEIAVFDRVLTAEELKSLS 708          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A517ZRT5_9PLAN (Neutral/alkaline non-lysosomal ceramidase n=4 Tax=Symmachiella TaxID=2795780 RepID=A0A517ZRT5_9PLAN)

HSP 1 Score: 150 bits (379), Expect = 6.920e-40
Identity = 67/127 (52.76%), Postives = 91/127 (71.65%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTSHPGRLVYLHDG---ETVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLSGQ 372
            +FS G DHGL S  DHLG+GGT HPG+L++ H G   +T  G++ + RWTW+HVVF RDG+ VR+YL+G   PEI +       +  +++ +GGRGD +S+WEGRLDEIAVF R LS EE++ L+GQ
Sbjct:  583 LFSHGPDHGLGSNSDHLGLGGTEHPGKLIFRHGGAGAKTEVGKTTIPRWTWNHVVFERDGEFVRVYLNGQQQPEIGVKLPSGLLSPRKQVFWGGRGDNESNWEGRLDEIAVFPRVLSTEEIKTLAGQ 709          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A5Q4GG94_9PLAN (LamG domain-containing protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A5Q4GG94_9PLAN)

HSP 1 Score: 144 bits (364), Expect = 3.900e-38
Identity = 70/129 (54.26%), Postives = 91/129 (70.54%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGT-SHPGRLVYLHDGE-----TVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLSG 369
            MFSRGR +GL   GDHLGVGGT S PGRLV LH  +     T  G++++ERWTW+H+VFVRD Q+VR+YL+G + PEI        P+ ++ L FGGR D Q +WEGRLDE+AVF RA++ +E+   SG
Sbjct:  453 MFSRGRPYGLGPLGDHLGVGGTASEPGRLVLLHVDQAHAATTRVGRTEIERWTWNHLVFVRDQQQVRVYLNGDARPEIEAELPAGFPSDLDRLFFGGRSDNQDNWEGRLDEVAVFSRAVTIDEIAAWSG 581          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A3L7P4F1_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3L7P4F1_9BACT)

HSP 1 Score: 144 bits (364), Expect = 8.630e-38
Identity = 67/126 (53.17%), Postives = 90/126 (71.43%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTS-HPGRLVYLHDGET-VAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLSGQ 372
            +FSR  +H + + GDH+G+GGT+  PG+L++ H  +  V G++ ++RWTW HVVFVRD   VR+YL+G SDPEI +  S   PT  ++   GGR D QS+WEGRLDE AVF+RALS EE+R LS Q
Sbjct:  595 IFSRDENHAVGTSGDHVGIGGTATEPGKLIFQHGTDKPVVGRTVIDRWTWSHVVFVRDAGRVRVYLNGQSDPEIDVPCSAGFPTGFDQFFLGGRSDNQSNWEGRLDEAAVFNRALSTEEIRQLSVQ 720          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A5C6BI09_9BACT (Neutral/alkaline non-lysosomal ceramidase n=1 Tax=Planctomycetes bacterium Poly21 TaxID=2528014 RepID=A0A5C6BI09_9BACT)

HSP 1 Score: 142 bits (359), Expect = 4.130e-37
Identity = 68/127 (53.54%), Postives = 87/127 (68.50%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTSH-PGRLVYLHDGETV-----AGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLL 363
            MFSR  D+G+   GDH+G+GG  + PGRL++ H  +T      AG ++++RW WH +VFVRD  +VRIYL+G S PEI   S  + P   + L FGGR D  S+WEGRLDEIAVFDRALSA+EV  L
Sbjct:  585 MFSRDHDNGIGPAGDHVGIGGRGNEPGRLIFQHGADTAGASPAAGSTEIQRWKWHQMVFVRDRDQVRIYLNGNSQPEIDTESPAEFPIGFDRLFFGGRCDNDSNWEGRLDEIAVFDRALSADEVAAL 711          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: UPI0013D9E646 (hypothetical protein n=1 Tax=Candidatus Laterigemmans baculatus TaxID=2770505 RepID=UPI0013D9E646)

HSP 1 Score: 142 bits (358), Expect = 5.670e-37
Identity = 70/126 (55.56%), Postives = 90/126 (71.43%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTS-HPGRLVYLH-DGETVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAI-EELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLLSG 369
             FSRG +HGL   G+ LGVGGT  H G+L++   DG   AG++++ RWTW  V  VR+G EVR+YL+G  +PEI  T+S QTPTA  +E+  GGR D Q++WEGRLDEIA+FDR L  EEVR L+G
Sbjct:  587 FFSRGPNHGLAPAGEQLGVGGTGEHAGKLIFQQGDGSPTAGRTEIPRWTWAQVTMVREGDEVRVYLNGNEEPEIVATAS-QTPTAAAKEIFLGGRSDNQANWEGRLDEIALFDRPLRGEEVRALAG 711          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A5C5YBZ3_9BACT (Neutral/alkaline non-lysosomal ceramidase n=1 Tax=Rhodopirellula solitaria TaxID=2527987 RepID=A0A5C5YBZ3_9BACT)

HSP 1 Score: 141 bits (356), Expect = 1.080e-36
Identity = 67/127 (52.76%), Postives = 85/127 (66.93%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTSH-PGRLVYLHDGE-----TVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRALSAEEVRLL 363
            MFSRG DH L   GDHLG+GG  H PGRL++LH  E       AG + + RW W+HVV VRD + VR++L+G   PEI + S    P  ++ L FGGR D +S+WEGRLDEIAVFDRAL+ +E+  L
Sbjct:  585 MFSRGHDHDLGPGGDHLGLGGAEHHPGRLIFLHGDEGQGAKVAAGSTPLRRWNWNHVVLVRDREHVRVHLNGNPQPEIEVQSPGGFPAGLDRLFFGGRCDNESNWEGRLDEIAVFDRALTPDEITAL 711          
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Match: A0A2D6SX92_9PLAN (Uncharacterized protein (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2D6SX92_9PLAN)

HSP 1 Score: 141 bits (355), Expect = 1.420e-36
Identity = 67/116 (57.76%), Postives = 86/116 (74.14%), Query Frame = 1
Query:    1 MFSRGRDHGLTSQGDHLGVGGTS-HPGRLVYLH--DG-ETVAGQSQVERWTWHHVVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWEGRLDEIAVFDRA 336
            MFSRG DHGL+  GDHLG+GGT+  PG+LV+LH  DG + + G++ VERWTW+H V VRDG+ VR+YL+G   PEIA  ++   P + + L  GGR D QS+WEGRLDE+AVF RA
Sbjct:  589 MFSRGLDHGLSPHGDHLGLGGTATEPGKLVFLHGDDGAKPIVGRTAVERWTWNHAVLVRDGENVRVYLNGNPQPEIATKAAAGFPASFDRLFLGGRCDNQSNWEGRLDEVAVFARA 704          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig104467.962.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7Y5SMK9_9BACT2.290e-4361.72LamGL domain-containing protein n=1 Tax=Pirellulal... [more]
A0A7C7UZM2_9PLAN4.230e-4253.91LamG domain-containing protein n=1 Tax=Planctomyce... [more]
A0A517VW69_9PLAN5.030e-4057.60Neutral/alkaline non-lysosomal ceramidase n=2 Tax=... [more]
A0A517ZRT5_9PLAN6.920e-4052.76Neutral/alkaline non-lysosomal ceramidase n=4 Tax=... [more]
A0A5Q4GG94_9PLAN3.900e-3854.26LamG domain-containing protein n=1 Tax=Planctomyce... [more]
A0A3L7P4F1_9BACT8.630e-3853.17Uncharacterized protein n=1 Tax=Planctomycetes bac... [more]
A0A5C6BI09_9BACT4.130e-3753.54Neutral/alkaline non-lysosomal ceramidase n=1 Tax=... [more]
UPI0013D9E6465.670e-3755.56hypothetical protein n=1 Tax=Candidatus Laterigemm... [more]
A0A5C5YBZ3_9BACT1.080e-3652.76Neutral/alkaline non-lysosomal ceramidase n=1 Tax=... [more]
A0A2D6SX92_9PLAN1.420e-3657.76Uncharacterized protein (Fragment) n=1 Tax=Plancto... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig104467contigM-pyrifera_M_contig104467:204..578 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score137.1
Seed ortholog evalue5.6e-30
Seed eggNOG ortholog344747.PM8797T_27250
KEGG koko:K18195
Hectar predicted targeting categoryother localisation
EggNOG free text desc.lyase activity
EggNOG OGs2IXDY@203682,COG3356@1,COG3356@2,COG4625@1,COG4625@2
EC4.2.2.23
COG Functional cat.G
CAZyPL4
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons1
Model size375
Cds size375
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815551.9138792-CDS-M-pyrifera_M_contig104467:203..5781622815551.9138792-CDS-M-pyrifera_M_contig104467:203..578Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104467 204..578 -
1692277460.1809168-CDS-M-pyrifera_M_contig104467:203..5781692277460.1809168-CDS-M-pyrifera_M_contig104467:203..578Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104467 204..578 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig104467.962.1prot_M-pyrifera_M_contig104467.962.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig104467 204..578 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig104467.962.1

>prot_M-pyrifera_M_contig104467.962.1 ID=prot_M-pyrifera_M_contig104467.962.1|Name=mRNA_M-pyrifera_M_contig104467.962.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=125bp
MFSRGRDHGLTSQGDHLGVGGTSHPGRLVYLHDGETVAGQSQVERWTWHH
VVFVRDGQEVRIYLDGGSDPEIAITSSKQTPTAIEELIFGGRGDQQSSWE
GRLDEIAVFDRALSAEEVRLLSGQ*
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mRNA from alignment at M-pyrifera_M_contig104467:204..578-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig104467.962.1 ID=mRNA_M-pyrifera_M_contig104467.962.1|Name=mRNA_M-pyrifera_M_contig104467.962.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=375bp|location=Sequence derived from alignment at M-pyrifera_M_contig104467:204..578- (Macrocystis pyrifera P11B4 male)
ATGTTCTCGCGAGGAAGAGATCACGGTCTCACTTCGCAGGGTGATCATCT GGGAGTCGGCGGGACGTCCCACCCGGGTCGCCTCGTCTACCTGCATGATG GAGAGACGGTCGCTGGTCAGTCGCAAGTTGAACGCTGGACTTGGCACCAC GTGGTCTTCGTTCGTGATGGTCAAGAGGTCCGTATTTATCTTGATGGTGG GTCGGATCCCGAGATTGCGATCACGTCCTCGAAACAAACACCTACAGCGA TTGAAGAGTTGATCTTCGGTGGACGCGGCGATCAGCAGTCGAGCTGGGAA GGCCGTCTTGATGAGATTGCTGTCTTTGATCGAGCATTGAGTGCTGAAGA GGTTCGCTTGCTGTCTGGCCAATAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig104467:204..578-

>mRNA_M-pyrifera_M_contig104467.962.1 ID=mRNA_M-pyrifera_M_contig104467.962.1|Name=mRNA_M-pyrifera_M_contig104467.962.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=750bp|location=Sequence derived from alignment at M-pyrifera_M_contig104467:204..578- (Macrocystis pyrifera P11B4 male)
ATGTTCTCGCGAGGAAGAGATCACGGTCTCACTTCGCAGGGTGATCATCT
GGGAGTCGGCGGGACGTCCCACCCGGGTCGCCTCGTCTACCTGCATGATG
GAGAGACGGTCGCTGGTCAGTCGCAAGTTGAACGCTGGACTTGGCACCAC
GTGGTCTTCGTTCGTGATGGTCAAGAGGTCCGTATTTATCTTGATGGTGG
GTCGGATCCCGAGATTGCGATCACGTCCTCGAAACAAACACCTACAGCGA
TTGAAGAGTTGATCTTCGGTGGACGCGGCGATCAGCAGTCGAGCTGGGAA
GGCCGTCTTGATGAGATTGCTGTCTTTGATCGAGCATTGAGTGCTGAAGA
GGTTCGCTTGCTGTCTGGCCAATAAATGTTCTCGCGAGGAAGAGATCACG
GTCTCACTTCGCAGGGTGATCATCTGGGAGTCGGCGGGACGTCCCACCCG
GGTCGCCTCGTCTACCTGCATGATGGAGAGACGGTCGCTGGTCAGTCGCA
AGTTGAACGCTGGACTTGGCACCACGTGGTCTTCGTTCGTGATGGTCAAG
AGGTCCGTATTTATCTTGATGGTGGGTCGGATCCCGAGATTGCGATCACG
TCCTCGAAACAAACACCTACAGCGATTGAAGAGTTGATCTTCGGTGGACG
CGGCGATCAGCAGTCGAGCTGGGAAGGCCGTCTTGATGAGATTGCTGTCT
TTGATCGAGCATTGAGTGCTGAAGAGGTTCGCTTGCTGTCTGGCCAATAA
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