mRNA_M-pyrifera_M_contig104432.945.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig104432.945.1
Unique NamemRNA_M-pyrifera_M_contig104432.945.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A3D5DI27_9GAMM (Uncharacterized protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DI27_9GAMM)

HSP 1 Score: 184 bits (466), Expect = 1.590e-54
Identity = 94/194 (48.45%), Postives = 133/194 (68.56%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLA 582
            LAR++A  +D++RS+GLA INAITQS+F ++       ++SM G++L  GD LG++G FPSLVRQA   GV  TV+ERKHH++  +    VTLD   L DCNK+ICTAATLIND++D++L YC   +++ ++GPSASF P+P+F RGV++VGG+R+ D    I   + G GL     RY +  + + GT  L A
Sbjct:   83 LARKYADTNDMDRSVGLATINAITQSVFIRSNVGSCSTSDSMGGISLVPGDRLGMVGNFPSLVRQARKGGVPVTVVERKHHMLNRTQGIQVTLDSKVLRDCNKIICTAATLINDSVDEVLEYCLHADMVVMIGPSASFFPDPMFSRGVAVVGGTRVLDPVGLIDGLKMGQGLADHVYRYTLSDNEYVGTLQLCA 276          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A521RFE8_9GAMM (Uncharacterized protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A521RFE8_9GAMM)

HSP 1 Score: 168 bits (425), Expect = 2.610e-48
Identity = 84/185 (45.41%), Postives = 123/185 (66.49%), Query Frame = 1
Query:   25 DDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALL 579
            D  + S+GLA INA+T SLF +AGY  PPA NS+ G+  E GD +G++GYFPSLV +     +  TV+E+K   V E     VTLDP  L DCNKV+ TA+TL+N++ID++LA  +G E +A++GP+A F P+PLF+RGV+ +GGS + DA  A+   ++   +    R++VI    +PG  ++L
Sbjct:   92 DPAKCSLGLATINALTSSLFRRAGYVRPPAGNSLGGMQFENGDRVGMVGYFPSLVEKLRERRIPLTVLEKKSEYVEEGELFRVTLDPAGLGDCNKVVSTASTLLNNSIDEILAAASGAEELAIIGPTAGFFPDPLFKRGVTAIGGSEVIDAALALQRLKADERVGDAGRKFVIMARDYPGIESIL 276          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: UPI001F40F4D2 (DUF364 domain-containing protein n=1 Tax=Hydrogenophaga sp. SL48 TaxID=2806347 RepID=UPI001F40F4D2)

HSP 1 Score: 167 bits (422), Expect = 8.000e-48
Identity = 89/199 (44.72%), Postives = 122/199 (61.31%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGD----CVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLAR 585
            LA R    + +ER++ LAAINA+T S++ + GYEPPPA NS+  + L A DHLG+IG+FP LVR+    G R +V+E    +V    +      + LD   LADCN V+ T+  L+NDT+DDMLA        AV+GPSA   P+ LFERGV+++GG+RI D     A   +G+     +R++ IER SWPG  AL  R
Sbjct:   90 LASRLLDGNAVERALALAAINAMTDSVWRRVGYEPPPAGNSLGDVELAATDHLGMIGFFPPLVRRVAEAGGRLSVVEMNEAMVARQRERFPGVTIGLDRALLADCNLVVGTSTMLLNDTLDDMLAAAPRATRFAVIGPSAGLWPDTLFERGVTLLGGTRITDPAGFAAAMAAGASWSGATRKFAIERGSWPGWRALTQR 288          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A1G0KA03_9GAMM (DUF364 domain-containing protein n=2 Tax=unclassified Gammaproteobacteria TaxID=33811 RepID=A0A1G0KA03_9GAMM)

HSP 1 Score: 164 bits (414), Expect = 1.240e-46
Identity = 92/186 (49.46%), Postives = 121/186 (65.05%), Query Frame = 1
Query:   25 DDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCV-VTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALL 579
            D+ + SIGLAAINAITQ +F  A Y+   A NSM  L     DH+G++GYFPSLV +     +R TVIE+K   + ESGD V +T +P RL  CNKVI TAATL+N+TID ML Y +G E I V+GP+A F P+PLF R V+ +GGS I      I   +   G+ A + +Y+IE+ ++PG A LL
Sbjct:   90 DEADCSIGLAAINAITQYIFRLADYKLAAAGNSMGALEFMPDDHIGMVGYFPSLVSKLRQRAIRLTVIEKKTKFL-ESGDLVNITTEPARLGTCNKVIITAATLLNETIDAMLDYASGAEQIVVVGPTAGFFPDPLFARRVTAIGGSEIILPEVLIDRLKRDQGMGASASKYLIEKDNYPGAAILL 274          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A7V7WYY8_9BURK (DUF364 domain-containing protein n=1 Tax=Burkholderiaceae bacterium TaxID=2030806 RepID=A0A7V7WYY8_9BURK)

HSP 1 Score: 163 bits (413), Expect = 1.570e-46
Identity = 87/196 (44.39%), Postives = 120/196 (61.22%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLARA 588
            LAR +AG    +R++G AA+NA+T  LF +A Y PP A  S+  L+L  GDHLG++G FP LV Q  A G R TV+E +  +        VTLDP RL  CNK++ T+  L+NDT+D +L  C   E +A++GP A   P+PLF RGV+++GGSR+ DA A     + G    A   ++ I R+ +PG   LLARA
Sbjct:   83 LARSYAGDSGAQRALGFAAVNALTSHLFRRARYVPPAAQGSLGDLDLRPGDHLGMVGLFPPLVPQVLATGARLTVVELRADVAGPRDGWEVTLDPNRLRRCNKILSTSTVLLNDTLDGVLGRCRHAERVALIGPGAGCLPDPLFSRGVTLLGGSRVVDATAYKTALREGEPWGAAVMKFAIARNGYPGFEPLLARA 278          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A2U0AID9_9GAMM (DUF364 domain-containing protein n=2 Tax=gamma proteobacterium symbiont of Ctena orbiculata TaxID=1968598 RepID=A0A2U0AID9_9GAMM)

HSP 1 Score: 163 bits (412), Expect = 1.940e-46
Identity = 87/196 (44.39%), Postives = 121/196 (61.73%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLARA 588
            LA  +   D + RS+GL AI+AITQSLF +A Y P    NSMA +     DH+G++GYFP LV +    GVR +VIE++   V++     VTLDP  LA+CNK++CTAATL+ND++D++LA+C   + +AV+GP+A   P+PLF RGV  +GGSR+          +          +Y IER  +PG   LL RA
Sbjct:   82 LAHYYGEQDPLARSMGLGAISAITQSLFRRADYMPDRQTNSMAKMAFSQTDHVGMVGYFPPLVERLRGRGVRLSVIEKRAEFVQQGDRFQVTLDPRVLAECNKILCTAATLLNDSLDEILAHCGQAQRVAVIGPTAGCLPDPLFSRGVDAIGGSRVAVPERLKQRLRDQLEWADAVEKYTIERDDYPGFEQLLLRA 277          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: H5WUI6_9BURK (Uncharacterized protein n=1 Tax=Burkholderiales bacterium JOSHI_001 TaxID=864051 RepID=H5WUI6_9BURK)

HSP 1 Score: 163 bits (412), Expect = 1.940e-46
Identity = 83/196 (42.35%), Postives = 128/196 (65.31%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLARA 588
            LAR++       R++G AA+NAIT+ LF +AG++PPPAA S+  L+L+ GDHLG++G+FP L+++    G + TV+E +  LV E     VTLD   L  CNKV+ T+  L+N T+D +LA+CAG + +A++GP A   P+ LF RG++++GGS IDD  +  A   +G      + ++ +   ++PG +ALLARA
Sbjct:   82 LARQYLEGQGSLRTLGFAAVNAITRHLFDRAGFKPPPAAGSVGDLDLQPGDHLGMVGFFPPLIQRVLDAGAQLTVVELRADLVGERDGFRVTLDAAELRHCNKVLSTSTVLLNQTLDAVLAHCAGAQRLAMVGPGAGCLPDALFARGLTLLGGSWIDDPASFKAALAAGDSWGPFATKFALRPDAYPGLSALLARA 277          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A521I2J6_9CHLR (Uncharacterized protein n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A521I2J6_9CHLR)

HSP 1 Score: 163 bits (412), Expect = 2.100e-46
Identity = 85/195 (43.59%), Postives = 125/195 (64.10%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLAR 585
            LAR FA +D ++R++G AAINA++Q LF+ A + P    NS+  LN + G+H+G+IG F  L+      G R TV+E  H LVR+  +  VTLDP  LADC++++ T A ++NDT+DD+LA C      AV+GP+AS  P+PLF RGV  +GG R+ +    +A  +SG+   A + +YVI R  +PG   L+A+
Sbjct:   80 LARGFATSDPVDRALGFAAINALSQQLFTHANWVPSANENSLGALNPQRGEHIGMIGLFMPLIPIIERSGARLTVLELDHALVRDEANFRVTLDPAELADCSQIVSTCAVMLNDTLDDVLAACRKARRFAVVGPTASCVPDPLFARGVDSLGGRRVIEREKFVAAIRSGNKWGAYASKYVIARDDYPGIEWLIAQ 274          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A2D6MS98_9DELT (Uncharacterized protein n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A2D6MS98_9DELT)

HSP 1 Score: 163 bits (412), Expect = 2.340e-46
Identity = 85/197 (43.15%), Postives = 123/197 (62.44%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIER-SSWPGTAALLARA 588
            +A+ + G    +RS+G+AAINAI+Q +  ++GY PP    ++A L L+ GDHLGL+GYFP LV QA A G+  TV+E     ++ES + VVTLDP  L  CNK++CT   LIN TID +L YC   E   ++GP+    P+PLF+RG++ +GG R+ D    + +       R  +RRY + R + +PG  ALL+ A
Sbjct:   84 VAQLYCGESGWQRSLGMAAINAISQFVLRESGYSPPGMGKTLAQLALKKGDHLGLVGYFPPLVEQARADGIHLTVLELDDRWLQESDEFVVTLDPKDLGTCNKLVCTGTALINQTIDSVLPYCRSAEHKLIVGPTVGCLPDPLFDRGLTALGGCRVVDLEKFLRLWAGQQKWRGATRRYELSRRTGYPGVEALLSAA 280          
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Match: A0A0S8AVW5_9PROT (Uncharacterized protein n=1 Tax=Betaproteobacteria bacterium SG8_39 TaxID=1703390 RepID=A0A0S8AVW5_9PROT)

HSP 1 Score: 162 bits (411), Expect = 2.590e-46
Identity = 87/197 (44.16%), Postives = 124/197 (62.94%), Query Frame = 1
Query:    1 LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAGDHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLADCNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSIVGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLARAA 591
            +AR +A  D + R++GLAA NAI+  +F QA Y P  A++S+    L  GDHLG+IG+F  LV +  A G R TV+E +  LV  + D  VTLDP +LA C K+I T++ L+NDT+DD+LA+       A++GPSAS  P+PLF RGV++ GG R+DD  A +   ++         +  I R ++PG  AL+ARAA
Sbjct:   80 IARGYAERDSVARALGLAAYNAISHHVFRQANYLPETASDSLGAFGLGPGDHLGMIGFFRVLVPRVRAAGARLTVLELRPELVETTPDYEVTLDPAKLAACTKIIATSSVLLNDTLDDVLAHGGHAREFALIGPSASGFPDPLFARGVTVAGGYRVDDPAALLRRARALEAWGDAGTKCAIRRDAYPGFEALIARAA 276          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig104432.945.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D5DI27_9GAMM1.590e-5448.45Uncharacterized protein n=1 Tax=Gammaproteobacteri... [more]
A0A521RFE8_9GAMM2.610e-4845.41Uncharacterized protein n=1 Tax=Gammaproteobacteri... [more]
UPI001F40F4D28.000e-4844.72DUF364 domain-containing protein n=1 Tax=Hydrogeno... [more]
A0A1G0KA03_9GAMM1.240e-4649.46DUF364 domain-containing protein n=2 Tax=unclassif... [more]
A0A7V7WYY8_9BURK1.570e-4644.39DUF364 domain-containing protein n=1 Tax=Burkholde... [more]
A0A2U0AID9_9GAMM1.940e-4644.39DUF364 domain-containing protein n=2 Tax=gamma pro... [more]
H5WUI6_9BURK1.940e-4642.35Uncharacterized protein n=1 Tax=Burkholderiales ba... [more]
A0A521I2J6_9CHLR2.100e-4643.59Uncharacterized protein n=1 Tax=Chloroflexi bacter... [more]
A0A2D6MS98_9DELT2.340e-4643.15Uncharacterized protein n=1 Tax=Deltaproteobacteri... [more]
A0A0S8AVW5_9PROT2.590e-4644.16Uncharacterized protein n=1 Tax=Betaproteobacteria... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig104432contigM-pyrifera_M_contig104432:47..643 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score163.3
Seed ortholog evalue1.2e-37
Seed eggNOG ortholog864051.BurJ1DRAFT_0529
KEGG koko:K09138
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Putative heavy-metal chelation
EggNOG OGs1KM4B@119065,1MV5Z@1224,2VPCD@28216,COG2014@1,COG2014@2
COG Functional cat.S
Best tax levelunclassified Burkholderiales
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons1
Model size597
Cds size597
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815551.0424256-CDS-M-pyrifera_M_contig104432:46..6431622815551.0424256-CDS-M-pyrifera_M_contig104432:46..643Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104432 47..643 -
1692277459.810473-CDS-M-pyrifera_M_contig104432:46..6431692277459.810473-CDS-M-pyrifera_M_contig104432:46..643Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104432 47..643 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig104432.945.1prot_M-pyrifera_M_contig104432.945.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig104432 47..643 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig104432.945.1

>prot_M-pyrifera_M_contig104432.945.1 ID=prot_M-pyrifera_M_contig104432.945.1|Name=mRNA_M-pyrifera_M_contig104432.945.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=199bp
LARRFAGADDIERSIGLAAINAITQSLFSQAGYEPPPAANSMAGLNLEAG
DHLGLIGYFPSLVRQAGALGVRTTVIERKHHLVRESGDCVVTLDPLRLAD
CNKVICTAATLINDTIDDMLAYCAGVEVIAVLGPSASFPPEPLFERGVSI
VGGSRIDDAGAAIAMQQSGSGLRACSRRYVIERSSWPGTAALLARAAA*
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mRNA from alignment at M-pyrifera_M_contig104432:47..643-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig104432.945.1 ID=mRNA_M-pyrifera_M_contig104432.945.1|Name=mRNA_M-pyrifera_M_contig104432.945.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=597bp|location=Sequence derived from alignment at M-pyrifera_M_contig104432:47..643- (Macrocystis pyrifera P11B4 male)
CTGGCCCGCCGTTTTGCCGGCGCTGATGACATCGAGCGCTCGATCGGACT CGCCGCCATCAACGCGATCACGCAAAGCCTGTTTTCACAGGCAGGCTATG AACCACCTCCGGCGGCGAACTCGATGGCGGGCCTGAACCTCGAGGCCGGC GACCACCTCGGGCTGATCGGCTATTTCCCTTCGCTCGTGCGCCAGGCCGG CGCGCTCGGCGTGCGCACGACCGTGATCGAGCGCAAGCATCACCTCGTGC GCGAATCCGGTGATTGCGTCGTGACGCTGGATCCGTTGCGGCTCGCGGAC TGCAACAAGGTGATCTGTACGGCCGCAACCCTGATCAACGACACGATCGA CGACATGCTTGCGTATTGCGCCGGGGTCGAGGTGATCGCGGTGCTCGGGC CGAGCGCAAGCTTCCCGCCCGAACCCCTGTTTGAACGCGGCGTCAGTATT GTCGGCGGTAGCCGGATTGACGATGCCGGCGCGGCGATCGCGATGCAGCA GTCCGGGTCGGGATTGCGCGCCTGTTCGCGCCGCTACGTGATCGAGCGAT CAAGCTGGCCCGGGACGGCGGCGTTGCTCGCCCGCGCCGCCGCGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig104432:47..643-

>mRNA_M-pyrifera_M_contig104432.945.1 ID=mRNA_M-pyrifera_M_contig104432.945.1|Name=mRNA_M-pyrifera_M_contig104432.945.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1194bp|location=Sequence derived from alignment at M-pyrifera_M_contig104432:47..643- (Macrocystis pyrifera P11B4 male)
CTGGCCCGCCGTTTTGCCGGCGCTGATGACATCGAGCGCTCGATCGGACT
CGCCGCCATCAACGCGATCACGCAAAGCCTGTTTTCACAGGCAGGCTATG
AACCACCTCCGGCGGCGAACTCGATGGCGGGCCTGAACCTCGAGGCCGGC
GACCACCTCGGGCTGATCGGCTATTTCCCTTCGCTCGTGCGCCAGGCCGG
CGCGCTCGGCGTGCGCACGACCGTGATCGAGCGCAAGCATCACCTCGTGC
GCGAATCCGGTGATTGCGTCGTGACGCTGGATCCGTTGCGGCTCGCGGAC
TGCAACAAGGTGATCTGTACGGCCGCAACCCTGATCAACGACACGATCGA
CGACATGCTTGCGTATTGCGCCGGGGTCGAGGTGATCGCGGTGCTCGGGC
CGAGCGCAAGCTTCCCGCCCGAACCCCTGTTTGAACGCGGCGTCAGTATT
GTCGGCGGTAGCCGGATTGACGATGCCGGCGCGGCGATCGCGATGCAGCA
GTCCGGGTCGGGATTGCGCGCCTGTTCGCGCCGCTACGTGATCGAGCGAT
CAAGCTGGCCCGGGACGGCGGCGTTGCTCGCCCGCGCCGCCGCGTAGCTG
GCCCGCCGTTTTGCCGGCGCTGATGACATCGAGCGCTCGATCGGACTCGC
CGCCATCAACGCGATCACGCAAAGCCTGTTTTCACAGGCAGGCTATGAAC
CACCTCCGGCGGCGAACTCGATGGCGGGCCTGAACCTCGAGGCCGGCGAC
CACCTCGGGCTGATCGGCTATTTCCCTTCGCTCGTGCGCCAGGCCGGCGC
GCTCGGCGTGCGCACGACCGTGATCGAGCGCAAGCATCACCTCGTGCGCG
AATCCGGTGATTGCGTCGTGACGCTGGATCCGTTGCGGCTCGCGGACTGC
AACAAGGTGATCTGTACGGCCGCAACCCTGATCAACGACACGATCGACGA
CATGCTTGCGTATTGCGCCGGGGTCGAGGTGATCGCGGTGCTCGGGCCGA
GCGCAAGCTTCCCGCCCGAACCCCTGTTTGAACGCGGCGTCAGTATTGTC
GGCGGTAGCCGGATTGACGATGCCGGCGCGGCGATCGCGATGCAGCAGTC
CGGGTCGGGATTGCGCGCCTGTTCGCGCCGCTACGTGATCGAGCGATCAA
GCTGGCCCGGGACGGCGGCGTTGCTCGCCCGCGCCGCCGCGTAG
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