mRNA_M-pyrifera_M_contig103966.841.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig103966.841.1
Unique NamemRNA_M-pyrifera_M_contig103966.841.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A2A2KYC6_9BILA (Uncharacterized protein n=1 Tax=Diploscapter pachys TaxID=2018661 RepID=A0A2A2KYC6_9BILA)

HSP 1 Score: 165 bits (418), Expect = 3.880e-49
Identity = 83/161 (51.55%), Postives = 104/161 (64.60%), Query Frame = 1
Query:   28 LFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSIK 510
            +FQ+VD DRSG IS+ ELQRALS+G    F+  T +LM+ MFD    G + F +F +LW Y+  W +CF SFD D SG I  GELQ A++RFGYR S  FY  LM  +D   T  + FD FI+LCV LQ LT  F  +DT R G  T  YE+FL+ VFS+K
Sbjct:   10 IFQSVDKDRSGLISADELQRALSNGTWNPFNPETCRLMIGMFDSDNDGAINFMEFEALWKYINDWTQCFRSFDLDSSGNIDKGELQNALTRFGYRLSDQFYNLLMMKFDRTHTSRINFDDFIQLCVVLQTLTAAFRDKDTDRDGVITIRYEEFLSMVFSLK 170          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A6V7XU38_9BILA (Hypothetical protein n=1 Tax=Meloidogyne enterolobii TaxID=390850 RepID=A0A6V7XU38_9BILA)

HSP 1 Score: 164 bits (416), Expect = 8.290e-49
Identity = 80/161 (49.69%), Postives = 107/161 (66.46%), Query Frame = 1
Query:   28 LFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSIK 510
            +FQ VDTDRSGRISS ELQRALS+G    F+  T ++M++MFD    G + F +F++LW Y+  W + F +FD D SG I  GEL +A+++FGYR S  FY  LM  +D   +G + FD FI+LCV LQ LT  F  +D+ R G    +YE+FLT VFS+K
Sbjct:   12 IFQTVDTDRSGRISSDELQRALSNGTWRPFNPETCRIMISMFDSDNDGGISFNEFQALWNYVNDWSRTFRTFDRDNSGNIDRGELMSALTQFGYRLSGPFYDMLMQKFDRTHSGRVNFDDFIQLCVVLQTLTAAFREKDSDRDGWIRIHYEEFLTMVFSMK 172          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A183TVT1_TOXCA (Uncharacterized protein n=3 Tax=Ascaridoidea TaxID=33256 RepID=A0A183TVT1_TOXCA)

HSP 1 Score: 164 bits (415), Expect = 1.070e-48
Identity = 87/168 (51.79%), Postives = 107/168 (63.69%), Query Frame = 1
Query:    1 MSAPDPAVSLFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFS 504
            M+ P  AV +F  VDTDRSGRIS+ ELQRALS+G    F+  T +LM+ MFD  R G + F +F +LW Y+ QW +CF SFD D SG I  GEL  A+S+FGYR S  F   LM  +D   T  + FD FI+LCV LQ LT  F  +DT R G  T  YE+FLT VF+
Sbjct:    1 MAFPSLAV-IFNNVDTDRSGRISADELQRALSNGTWTPFNPETCRLMIGMFDSDRDGAINFAEFSALWEYINQWTQCFRSFDRDGSGNIDKGELSAALSQFGYRLSDRFIDLLMTKFDRTHTHRVNFDDFIQLCVVLQTLTASFRDKDTDRDGVITVGYEEFLTMVFT 167          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A1I8BY90_MELHA (Uncharacterized protein n=4 Tax=Meloidogyne TaxID=189290 RepID=A0A1I8BY90_MELHA)

HSP 1 Score: 164 bits (415), Expect = 1.100e-48
Identity = 78/160 (48.75%), Postives = 106/160 (66.25%), Query Frame = 1
Query:   31 FQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSIK 510
            FQAVDTDRSG+IS+ ELQRALS+G    F+  T ++M++MFD+   G +GF +F+SLW Y+  W   F  +D D SG+I  GEL+ A+++FGYR S  FY   +  +D   +G + FD FI+LCV LQ LT  F  RDT + G    +YEDFL  VFS++
Sbjct:   11 FQAVDTDRSGKISANELQRALSNGSWRPFNQETCRMMISMFDKDNDGGIGFGEFQSLWAYINDWSNTFRGYDRDNSGSIDKGELRNALTQFGYRLSDRFYNMAIQKFDRSHSGSIKFDDFIQLCVVLQTLTAAFRERDTDQDGVIKIHYEDFLLMVFSLR 170          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: UPI00193A636E (peflin-like isoform X1 n=2 Tax=Styela clava TaxID=7725 RepID=UPI00193A636E)

HSP 1 Score: 165 bits (417), Expect = 4.890e-48
Identity = 84/162 (51.85%), Postives = 107/162 (66.05%), Query Frame = 1
Query:   13 DPAV-SLFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTA 495
            DPAV + FQAVD DRSGRIS+ ELQ+AL++     FSI+T   M+ +FDR  SG +   +F  LWG++ QW+  F SFD DRSGTIS  EL TA+SR GY  SP F    M+ +D DR G L F+ FI  C+ LQ  T HF  +DT R+G+  FNY+DF+ A
Sbjct:   76 DPAVLAWFQAVDADRSGRISALELQQALTNNDWTRFSIATCYGMIGLFDRDFSGTIDINEFGQLWGFINQWRGVFSSFDQDRSGTISHQELHTAISRLGYNLSPTFVNIAMYKFDHDRRGSLNFEGFINCCMMLQQCTHHFQMKDTQRSGRGQFNYDDFMYA 237          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: Q5TJE0_SUBDO (Apoptosis-linked gene 2 n=1 Tax=Suberites domuncula TaxID=55567 RepID=Q5TJE0_SUBDO)

HSP 1 Score: 163 bits (412), Expect = 7.640e-48
Identity = 81/162 (50.00%), Postives = 106/162 (65.43%), Query Frame = 1
Query:   28 LFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSIKA 513
            +FQ VD DRSG ISS ELQ+ALS+G    F+  T++LM+ MFDR RSG + F++F SLW Y+  W+  F S+D D SG+I   EL+TA++ FGYR S  FY  L+  +D    G + FD FI+ CV +Q LT  F   DT R G  + +YE FLT VFS+KA
Sbjct:   20 IFQKVDKDRSGAISSDELQQALSNGSWTAFNPETIRLMIGMFDRDRSGTINFQEFGSLWKYVQDWQTTFRSYDRDNSGSIDKTELKTALTNFGYRLSDQFYELLIRKFDRGGKGTVAFDDFIQCCVVIQTLTNSFKGFDTNRNGWISISYEQFLTLVFSLKA 181          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: UPI001B88B42A (programmed cell death protein 6-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B88B42A)

HSP 1 Score: 162 bits (410), Expect = 8.610e-48
Identity = 79/162 (48.77%), Postives = 108/162 (66.67%), Query Frame = 1
Query:   28 LFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSIKA 513
            +FQ VD DRSG ISS ELQ+ALS+G    F+  TV+LM+ MFDR +SG + F++F +LW Y+ +W+  F S+D D SG+I   EL+TA++ FGYR S  FY  L+  +D   +G++ FD FI+ CV +Q LT  F   DT R+G    +YE FL  VFS+KA
Sbjct:   20 IFQKVDKDRSGAISSDELQQALSNGTWTPFNPETVRLMIGMFDRDQSGTINFQEFGALWKYIQEWQTTFRSYDRDNSGSIDQSELKTALTNFGYRLSDRFYGLLIRKFDRTGSGNIAFDDFIQCCVVIQTLTNSFKAYDTNRSGWIRISYEQFLMLVFSLKA 181          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A814YKK3_ADIRI (Hypothetical protein n=5 Tax=Adineta ricciae TaxID=249248 RepID=A0A814YKK3_ADIRI)

HSP 1 Score: 162 bits (411), Expect = 9.030e-48
Identity = 80/161 (49.69%), Postives = 105/161 (65.22%), Query Frame = 1
Query:   28 LFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSIK 510
            +FQ VDTDRSG ISS ELQ ALS+G  + F+  T+++M+ MFDR  S  + F++FR LW Y+  W+KCF  FD D SG I   EL+TA+S FGYR S  FY  L+  +D    G++ FD FI+ CV +Q LT  F   D  + G+ T  YEDFLT VFS++
Sbjct:   28 IFQKVDTDRSGSISSNELQAALSNGTWSPFNPETIRMMIGMFDRDESSTIDFQEFRLLWKYVSDWEKCFKQFDLDGSGAIDKHELKTALSSFGYRLSDNFYELLIKRFDRTGRGNVAFDDFIQACVSIQTLTNAFRHFDRQQIGQITIGYEDFLTLVFSLR 188          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A1I7VCE8_LOALO (Programmed cell death protein 6 n=2 Tax=Loa loa TaxID=7209 RepID=A0A1I7VCE8_LOALO)

HSP 1 Score: 161 bits (407), Expect = 2.040e-47
Identity = 83/170 (48.82%), Postives = 107/170 (62.94%), Query Frame = 1
Query:    1 MSAPDPAVS-LFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFSI 507
            M+ P P++  +F +VDTDRSGRIS  ELQRALS+G    F+  T +LM++MFD  R G + F +F +LW Y+ QW +CF SFD D SG I   EL  A+S+FGYR S  F   LM  +D   T  + FD FI+LCV LQ LT  F  +DT R G  T  YE++LT  F +
Sbjct:    1 MALPIPSLQHIFASVDTDRSGRISVDELQRALSNGTWNPFNPETCRLMISMFDSDRDGAINFNEFSALWDYINQWTQCFRSFDIDGSGNIDKRELSMALSKFGYRLSDRFIGLLMMKFDRTHTHHINFDDFIQLCVVLQTLTAAFRDKDTDRDGVITIGYEEYLTMAFVV 170          
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Match: A0A0N5CM05_THECL (Uncharacterized protein n=1 Tax=Thelazia callipaeda TaxID=103827 RepID=A0A0N5CM05_THECL)

HSP 1 Score: 160 bits (406), Expect = 2.560e-47
Identity = 80/168 (47.62%), Postives = 108/168 (64.29%), Query Frame = 1
Query:    1 MSAPDPAVSLFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTMFDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSRFGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTTRTGKATFNYEDFLTAVFS 504
            MS P+P +++F +VDTD SG+ISS ELQRALS+G    F+  T +LM++MFD    G + F++F +LW Y+ QW +CF SFD D SG I   E+  A+S+FGYR S  F   LM  +D   T  + FD FI+LCV LQ LT  F  +D+ R G  T  YE++L  VF+
Sbjct:    1 MSVPNPLLNIFISVDTDASGKISSDELQRALSNGTWNPFNPETCRLMISMFDSDHDGAINFDEFSALWNYINQWTQCFRSFDVDGSGNIDKREMSLALSQFGYRLSDRFIDLLMTKFDRTHTHRINFDDFIQLCVVLQTLTAAFRDKDSDRDGFITIGYEEYLFMVFT 168          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig103966.841.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2A2KYC6_9BILA3.880e-4951.55Uncharacterized protein n=1 Tax=Diploscapter pachy... [more]
A0A6V7XU38_9BILA8.290e-4949.69Hypothetical protein n=1 Tax=Meloidogyne enterolob... [more]
A0A183TVT1_TOXCA1.070e-4851.79Uncharacterized protein n=3 Tax=Ascaridoidea TaxID... [more]
A0A1I8BY90_MELHA1.100e-4848.75Uncharacterized protein n=4 Tax=Meloidogyne TaxID=... [more]
UPI00193A636E4.890e-4851.85peflin-like isoform X1 n=2 Tax=Styela clava TaxID=... [more]
Q5TJE0_SUBDO7.640e-4850.00Apoptosis-linked gene 2 n=1 Tax=Suberites domuncul... [more]
UPI001B88B42A8.610e-4848.77programmed cell death protein 6-like n=1 Tax=Gigan... [more]
A0A814YKK3_ADIRI9.030e-4849.69Hypothetical protein n=5 Tax=Adineta ricciae TaxID... [more]
A0A1I7VCE8_LOALO2.040e-4748.82Programmed cell death protein 6 n=2 Tax=Loa loa Ta... [more]
A0A0N5CM05_THECL2.560e-4747.62Uncharacterized protein n=1 Tax=Thelazia callipaed... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig103966contigM-pyrifera_M_contig103966:39..615 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeNematoda
Seed ortholog score164.5
Seed ortholog evalue4.5e-38
Seed eggNOG ortholog281687.CJA13983
Hectar predicted targeting categoryother localisation
EggNOG free text desc.EF-hand domain
EggNOG OGs1KVG7@119089,38DBU@33154,3BFNP@33208,3CS7A@33213,40CUH@6231,40U08@6236,KOG0037@1,KOG0037@2759
COG Functional cat.T
Best tax levelRhabditida
Best eggNOG OGNA|NA|NA
Exons2
Model size516
Cds size516
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815545.3851063-CDS-M-pyrifera_M_contig103966:38..1871622815545.3851063-CDS-M-pyrifera_M_contig103966:38..187Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103966 39..187 -
1692277457.3514338-CDS-M-pyrifera_M_contig103966:38..1871692277457.3514338-CDS-M-pyrifera_M_contig103966:38..187Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103966 39..187 -
1622815545.3998458-CDS-M-pyrifera_M_contig103966:248..6151622815545.3998458-CDS-M-pyrifera_M_contig103966:248..615Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103966 249..615 -
1692277457.3607569-CDS-M-pyrifera_M_contig103966:248..6151692277457.3607569-CDS-M-pyrifera_M_contig103966:248..615Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103966 249..615 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig103966.841.1prot_M-pyrifera_M_contig103966.841.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig103966 39..615 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig103966.841.1

>prot_M-pyrifera_M_contig103966.841.1 ID=prot_M-pyrifera_M_contig103966.841.1|Name=mRNA_M-pyrifera_M_contig103966.841.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=172bp
MSAPDPAVSLFQAVDTDRSGRISSTELQRALSSGGMAMFSISTVKLMVTM
FDRSRSGEVGFEDFRSLWGYLVQWKKCFDSFDADRSGTISAGELQTAMSR
FGYRFSPAFYAHLMHVYDEDRTGDLGFDHFIRLCVELQILTRHFATRDTT
RTGKATFNYEDFLTAVFSIKA*
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mRNA from alignment at M-pyrifera_M_contig103966:39..615-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig103966.841.1 ID=mRNA_M-pyrifera_M_contig103966.841.1|Name=mRNA_M-pyrifera_M_contig103966.841.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=577bp|location=Sequence derived from alignment at M-pyrifera_M_contig103966:39..615- (Macrocystis pyrifera P11B4 male)
ATGTCTGCACCAGACCCCGCCGTATCTCTTTTCCAGGCCGTGGATACCGA CAGGTCCGGCAGGATCAGCTCCACGGAGCTCCAGCGCGCTCTGTCGTCTG GAGGCATGGCCATGTTCAGCATCAGCACAGTGAAGTTGATGGTGACCATG TTTGACCGCTCTCGCTCCGGCGAGGTTGGCTTTGAGGACTTCCGTTCGCT GTGGGGATACCTGGTGCAGTGGAAGAAGTGCTTCGACTCCTTCGACGCGG ATCGCAGCGGAACTATCTCCGCCGGAGAGTTGCAGACTGCCATGTCTCGC TTTGGCTACCGCTTCTCGCCCGCGTTCTACGCGCACCTCATGCACGTATA CGATGAAGACCGCACTGGTATGTTTGTGCACGCCTTTGCGCCGCGTTTGT TGCCTGACTCACCGAGCAACTCGTGCAGGCGACTTGGGCTTTGATCACTT TATCCGCCTCTGCGTCGAGCTGCAGATCCTCACGCGCCACTTTGCAACGC GCGACACAACACGCACTGGAAAGGCCACCTTCAACTACGAAGACTTCCTC ACTGCCGTCTTCAGCATCAAGGCATAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig103966:39..615-

>mRNA_M-pyrifera_M_contig103966.841.1 ID=mRNA_M-pyrifera_M_contig103966.841.1|Name=mRNA_M-pyrifera_M_contig103966.841.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1032bp|location=Sequence derived from alignment at M-pyrifera_M_contig103966:39..615- (Macrocystis pyrifera P11B4 male)
ATGTCTGCACCAGACCCCGCCGTATCTCTTTTCCAGGCCGTGGATACCGA
CAGGTCCGGCAGGATCAGCTCCACGGAGCTCCAGCGCGCTCTGTCGTCTG
GAGGCATGGCCATGTTCAGCATCAGCACAGTGAAGTTGATGGTGACCATG
TTTGACCGCTCTCGCTCCGGCGAGGTTGGCTTTGAGGACTTCCGTTCGCT
GTGGGGATACCTGGTGCAGTGGAAGAAGTGCTTCGACTCCTTCGACGCGG
ATCGCAGCGGAACTATCTCCGCCGGAGAGTTGCAGACTGCCATGTCTCGC
TTTGGCTACCGCTTCTCGCCCGCGTTCTACGCGCACCTCATGCACGTATA
CGATGAAGACCGCACTGATGTCTGCACCAGACCCCGCCGTATCTCTTTTC
CAGGCCGTGGATACCGACAGGTCCGGCAGGATCAGCTCCACGGAGCTCCA
GCGCGCTCTGTCGTCTGGAGGCATGGCCATGTTCAGCATCAGCACAGTGA
AGTTGATGGTGACCATGTTTGACCGCTCTCGCTCCGGCGAGGTTGGCTTT
GAGGACTTCCGTTCGCTGTGGGGATACCTGGTGCAGTGGAAGAAGTGCTT
CGACTCCTTCGACGCGGATCGCAGCGGAACTATCTCCGCCGGAGAGTTGC
AGACTGCCATGTCTCGCTTTGGCTACCGCTTCTCGCCCGCGTTCTACGCG
CACCTCATGCACGTATACGATGAAGACCGCACTGGCGACTTGGGCTTTGA
TCACTTTATCCGCCTCTGCGTCGAGCTGCAGATCCTCACGCGCCACTTTG
CAACGCGCGACACAACACGCACTGGAAAGGCCACCTTCAACTACGAAGAC
TTCCTCACTGCCGTCTTCAGCATCAAGGCATAGGCGACTTGGGCTTTGAT
CACTTTATCCGCCTCTGCGTCGAGCTGCAGATCCTCACGCGCCACTTTGC
AACGCGCGACACAACACGCACTGGAAAGGCCACCTTCAACTACGAAGACT
TCCTCACTGCCGTCTTCAGCATCAAGGCATAG
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