mRNA_M-pyrifera_M_contig103257.683.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig103257.683.1
Unique NamemRNA_M-pyrifera_M_contig103257.683.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A7V1IGF6_9DELT (TetR/AcrR family transcriptional regulator n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A7V1IGF6_9DELT)

HSP 1 Score: 134 bits (337), Expect = 1.440e-35
Identity = 84/170 (49.41%), Postives = 114/170 (67.06%), Query Frame = 1
Query:   25 KRDRTRRKLLDAGLEVMAERGEALTASDVVNAADVSNGTFYNHFLDREDFIQALALESLEVITTGSADDTEGA-DPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGV--RQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVS----PDAPAEA 513
            K++RTRR+L+ AGL V+AE+G+ LT SDVV  A VSNGTFYN+F+DR+D ++ LA +S   ++  +A   EG  DPA RFA+ATTRVL+ A  +  W R +LRLA    P  SG+   ++L  DL EG+  GRF  G D  T+D V+G I+ +IRR+V     PDAP +A
Sbjct:   25 KKERTRRQLIAAGLRVLAEKGQGLTVSDVVAEAGVSNGTFYNYFVDRDDLMEVLAEQS--ALSLAAAAAMEGVEDPARRFALATTRVLQHAIEDDTWARVMLRLA--GRP-GSGIDLSRYLREDLEEGYALGRFETGADDATLDQVTGLIVMTIRRIVEGRAGPDAPRQA 189          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: UPI0019FBCE46 (TetR/AcrR family transcriptional regulator n=1 Tax=filamentous cyanobacterium LEGE 11480 TaxID=2777977 RepID=UPI0019FBCE46)

HSP 1 Score: 99.8 bits (247), Expect = 1.120e-22
Identity = 65/185 (35.14%), Postives = 103/185 (55.68%), Query Frame = 1
Query:   25 KRDRTRRKLLDAGLEVMAERG-EALTASDVVNAADVSNGTFYNHFLDREDFIQALALESLEVITTGS-ADDTEGADPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVSPDAPAEAAGNDAIPTVVSRL-LEAIGV 570
            KR+RTR KLL+A  +V A +  +A+T  D+++AA V+ GTFYN+F  RED ++A+     E +     ++ T  ADPA R A A  + L  A R+  WG  I+R+   A PL + + Q + +DL  G +  RF        +DLV GT + +IR ++    P++       P  +++L L+ +GV
Sbjct:   13 KRERTRAKLLEAAYQVFAAKELDAVTIDDIISAAGVARGTFYNYFETREDVLKAVVASLAEAMNQAVWSESTAIADPAERMAFAIRQFLHQAKRDATWGWLIVRIGLVAAPLTATIHQGVRSDLEAGIQQQRFQVDSVQAAIDLVMGTGLMAIRSILEGQTPSD------YPEQIAKLILQTLGV 191          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A1Q3L5E1_9PROT (HTH tetR-type domain-containing protein n=2 Tax=Proteobacteria TaxID=1224 RepID=A0A1Q3L5E1_9PROT)

HSP 1 Score: 91.3 bits (225), Expect = 1.630e-19
Identity = 65/192 (33.85%), Postives = 102/192 (53.12%), Query Frame = 1
Query:    1 TMNKPRPPKRDRTRRKLLDAGLEVMAERGEALTA-SDVVNAADVSNGTFYNHFLDREDFIQALALE-SLEVITTGSADDTEGADPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVSPDAPAEAAGNDAIPTVVSRLLEAIGV 570
            T  +PR PKR+RTRR+++ A +EVMAERG A  A  ++ + A+V+ GTFYNHF D+ D ++A+A+  S E++          A  + R      R L  A   P     +L LA  +  +   + + +LAD+  G R   F    +   VDLV G IM ++R++     PA     D +  VV+ +L+ +G+
Sbjct:    4 TKPRPRLPKRERTRRQIISAAIEVMAERGLAQFAVQEIADRAEVTTGTFYNHFRDKADIVEAVAIRFSSEMMEAAEERRRTLASGSERLVDGCRRYLSIAREAPSTALLVLELAIASPRMLETIGEFVLADVRRGVRQKEFRIFGEQAAVDLVHGYIMLAMRQIALRPMPA---AYDRV--VVATILQGLGL 190          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A418WT66_9PROT (TetR/AcrR family transcriptional regulator n=1 Tax=Oleomonas cavernae TaxID=2320859 RepID=A0A418WT66_9PROT)

HSP 1 Score: 88.2 bits (217), Expect = 4.110e-18
Identity = 65/179 (36.31%), Postives = 88/179 (49.16%), Query Frame = 1
Query:    4 MNKPRPPKRDR-TRRKLLDAGLEVMAERG-EALTASDVVNAADVSNGTFYNHFLDREDFIQALALESLEVITTGSADDTEG-ADPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVSPD--APAEAAGND 525
            M +PR  +R R TR +LL+A LE+MAERG E +  +++  AADV  G+FYNHF  +E    AL     E          EG  DPA   ++     +  A  EP+WGR ++R       L  G+   L  D+  G   GRFT  D   T   V GT++ +I   +  D  A AE  G D
Sbjct:   13 MREPRGARRKRETRARLLEAALELMAERGVEGVAINEITEAADVGFGSFYNHFKSKEAIYDALIDTVFEEFGDTLDRLAEGIGDPAEYISICIRHAIRRAGHEPVWGRFLVRQGLSGQMLSRGLGPRLQRDIRRGLDAGRFTADDPVLTFIAVGGTVLTAISAQLQSDNAAVAEQLGAD 191          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A8J3E314_9PROT (Putative TetR-family transcriptional regulator n=1 Tax=Aliidongia dinghuensis TaxID=1867774 RepID=A0A8J3E314_9PROT)

HSP 1 Score: 88.6 bits (218), Expect = 4.430e-18
Identity = 64/160 (40.00%), Postives = 86/160 (53.75%), Query Frame = 1
Query:   13 PRPPKRDR-TRRKLLDAGLEVMAERG-EALTASDVVNAADVSNGTFYNHFLDREDFIQALALESLEVITTGSADDT---EGADPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASI 477
            PR  +R R TR +LLDA LE+MAE+G E +  +++  AADV  G+FYNHF  +E    AL     E    G+A D    + ADPA   +V+    L  A REP+WGR ++R    A  L  G+ Q LL D+ +G    RF   D         GT++ASI
Sbjct:   28 PRGERRKRETRSRLLDAALELMAEKGMEGVAINEITEAADVGFGSFYNHFESKEAIYAALLDAVFEEF--GNALDRLTQDIADPAEVISVSVRHTLRRAQREPVWGRFLMREGFSARVLERGLGQRLLRDIEKGVNEKRFPNDDILMMSTSAGGTVLASI 185          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A2Z4UQ20_9HYPH (Putative HTH-type transcriptional regulator YvdT n=1 Tax=Rhodobiaceae bacterium TaxID=2026785 RepID=A0A2Z4UQ20_9HYPH)

HSP 1 Score: 85.9 bits (211), Expect = 2.160e-17
Identity = 54/158 (34.18%), Postives = 80/158 (50.63%), Query Frame = 1
Query:   22 PKRDRTRRKLLDAGLEVMAERGEALTA-SDVVNAADVSNGTFYNHFLDREDFIQALALESLEVITTGSADDTEGADPAW-RFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMV 489
            PKRDRTR  L+D+ +EV+A++G  L   SD+   A ++NGTFYNHF D+++ +   A      +     ++    D A  R   ATTR +  A  E  WG  I+   +   P+   + Q L ADL  G   G F    D   +D ++  I+ SIR  +
Sbjct:   15 PKRDRTRLALIDSTVEVVAQKGMDLAKISDITEHAGLANGTFYNHFADKDEILTEAAQRVAVEMAREIDNEMRAVDSAVARMVTATTRAIRMAVSESAWGAVIIGALQHLPPVRGNILQFLRADLQLGVDQGMFDIELDEFFLDQIASLIIVSIRTQI 172          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: UPI000422497D (TetR/AcrR family transcriptional regulator n=1 Tax=Pseudonocardia spinosispora TaxID=103441 RepID=UPI000422497D)

HSP 1 Score: 85.9 bits (211), Expect = 2.160e-17
Identity = 52/157 (33.12%), Postives = 87/157 (55.41%), Query Frame = 1
Query:   28 RDRTRRKLLDAGLEVMAERGEA-LTASDVVNAADVSNGTFYNHFLDREDFIQALALESL-EVITTGSADDTEGADPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVS 492
            R+RTRR+LLDAG  ++ ERG A L   ++   ADV+ G+FYNHF  +E+ ++A+  ESL ++  T S      +DPA   A++  R +  A  EP + + ++ L+       + +  +  A L  G  +GRF   D   T+  V  + +A IR++++
Sbjct:   17 RERTRRRLLDAGRMLITERGVAGLRIQEITERADVALGSFYNHFSTKEELVEAVVTESLTDLAATASTPSAPDSDPAEAVAISCFRFIRLAYEEPDFAQLLVNLSSADALFDTTIHPYARAALERGIDSGRFVIPDLEVTLTAVISSALALIRQILA 173          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A2J7TC88_METSI (HTH tetR-type domain-containing protein n=2 Tax=Methylocella silvestris TaxID=199596 RepID=A0A2J7TC88_METSI)

HSP 1 Score: 85.1 bits (209), Expect = 7.380e-17
Identity = 67/189 (35.45%), Postives = 99/189 (52.38%), Query Frame = 1
Query:   28 RDRTRRKLLDAGLEVMAER-GEALTASDVVNAADVSNGTFYNHFLDREDFIQALALE-----SLEVITTGSADDTEGADPAWRFAVATTRVLEAATREPLWGRAILRLA---ECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVSPDAPAEAAGNDAIPTVVSRLLEAIG 567
            R RTR+ LL AG ++ AE+  +++T +D+   A+V+ G+FYNHF+D+E F +A+        +LE+    S +     DPA R A A   VL  A R P    A+L LA       PL+ GV     AD+AEG R GRF   D  T V +V   IM ++R ++S     +        +V + +L A+G
Sbjct:   24 RQRTRKSLLSAGQKLFAEQPADSVTITDITEEAEVAKGSFYNHFVDKEAFAEAIYTAVHGELNLEIDAANSGEK----DPAARIARALCLVLRHAQRHPEKLSALLSLAGRRSADAPLNDGVT----ADVAEGLRVGRFNGLDTKTGVLIVLSLIMETVRCILSGGFHEKTPAPALAGSVATAMLRALG 204          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: A0A8I1TG41_9HYPH (Helix-turn-helix transcriptional regulator n=1 Tax=Hyphomicrobiales bacterium TaxID=1909294 RepID=A0A8I1TG41_9HYPH)

HSP 1 Score: 83.2 bits (204), Expect = 2.520e-16
Identity = 60/188 (31.91%), Postives = 93/188 (49.47%), Query Frame = 1
Query:   25 KRDRTRRKLLDAGLEVMAERGEA-LTASDVVNAADVSNGTFYNHFLDREDFIQALALESLEVITTGSADDTEGA-----DPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVSPDAPAEAAGNDAIPTVVSRLLEAIGV 570
            +R R R++L++AG   +A  G A  T  D +  ADVS GTFYNHF  RED ++AL     E +      D + A     DPA R A  T  V+  A R+   G  I+ ++     ++  ++ +   DL  G   GRF + D  +  DLV GT+   +R ++S   P      D + ++   +L A+G+
Sbjct:   20 RRARMRQRLMEAGARAIAHHGMARATIDDFIAEADVSRGTFYNHFSMREDLLEAL----WERVGRDPFRDIQAACAIFADPAERLAAVTRSVMHRARRDATLGWLIVAMSADRRTVNEDLKAYPRPDLMLGRAAGRFAFDDLTSASDLVVGTVRTGLRALLSDTRPP-----DYVESLCKLILSALGM 198          
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Match: UPI0010AAC13D (TetR/AcrR family transcriptional regulator n=1 Tax=Nitratireductor sp. XY-223 TaxID=2561926 RepID=UPI0010AAC13D)

HSP 1 Score: 82.8 bits (203), Expect = 4.260e-16
Identity = 63/187 (33.69%), Postives = 98/187 (52.41%), Query Frame = 1
Query:   25 KRDRTRRKLLDAGLEVMAERGE-ALTASDVVNAADVSNGTFYNHFLDREDFIQALA---LESLEVITTGSADDTEGADPAWRFAVATTRVLEAATREPLWGRAILRLAECATPLHSG--VRQHLLADLAEGHRTGRFTYGDDPTTVDLVSGTIMASIRRMVSPDAPAEAAGNDAIPTVVSRLLEAIG 567
            +R RTR  ++ A +EV+AE+GE ++T  D ++ A VS GTFYN+F  +   + A+A   ++SL       + D E AD   R A    RVLE A R+P W  A+L       P   G     +LLADL  G   G F+  DD  +  ++ G++M  +R ++      +  G +    +VS++L A+G
Sbjct:   17 RRARTRSAIIQAAIEVIAEKGEESVTTQDFMDRAGVSRGTFYNYFDAKTSVLSAIADDLMDSLNAQIIEESQDIENADE--RLAWILHRVLERADRDPTWANALLVAVWRNAPNIVGETTMGYLLADLNLGRDQGLFSVMDDTVSASIIFGSMMFGMRAVLD-----QQYGVEQRRQLVSQILVALG 196          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig103257.683.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7V1IGF6_9DELT1.440e-3549.41TetR/AcrR family transcriptional regulator n=1 Tax... [more]
UPI0019FBCE461.120e-2235.14TetR/AcrR family transcriptional regulator n=1 Tax... [more]
A0A1Q3L5E1_9PROT1.630e-1933.85HTH tetR-type domain-containing protein n=2 Tax=Pr... [more]
A0A418WT66_9PROT4.110e-1836.31TetR/AcrR family transcriptional regulator n=1 Tax... [more]
A0A8J3E314_9PROT4.430e-1840.00Putative TetR-family transcriptional regulator n=1... [more]
A0A2Z4UQ20_9HYPH2.160e-1734.18Putative HTH-type transcriptional regulator YvdT n... [more]
UPI000422497D2.160e-1733.12TetR/AcrR family transcriptional regulator n=1 Tax... [more]
A0A2J7TC88_METSI7.380e-1735.45HTH tetR-type domain-containing protein n=2 Tax=Me... [more]
A0A8I1TG41_9HYPH2.520e-1631.91Helix-turn-helix transcriptional regulator n=1 Tax... [more]
UPI0010AAC13D4.260e-1633.69TetR/AcrR family transcriptional regulator n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig103257contigM-pyrifera_M_contig103257:84..653 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score93.2
Seed ortholog evalue1.4e-16
Seed eggNOG ortholog1235457.C404_11780
Hectar predicted targeting categoryother localisation
EggNOG free text desc.regulatory protein TetR
EggNOG OGs1K1BH@119060,1N6P0@1224,2VYIU@28216,COG1309@1,COG1309@2
COG Functional cat.K
Best tax levelBurkholderiaceae
Best eggNOG OGNA|NA|NA
Exons1
Model size570
Cds size567
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815537.2456996-UTR-M-pyrifera_M_contig103257:83..861622815537.2456996-UTR-M-pyrifera_M_contig103257:83..86Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig103257 84..86 +
1692277454.1503181-UTR-M-pyrifera_M_contig103257:83..861692277454.1503181-UTR-M-pyrifera_M_contig103257:83..86Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig103257 84..86 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815537.2641497-CDS-M-pyrifera_M_contig103257:86..6531622815537.2641497-CDS-M-pyrifera_M_contig103257:86..653Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103257 87..653 +
1692277454.163575-CDS-M-pyrifera_M_contig103257:86..6531692277454.163575-CDS-M-pyrifera_M_contig103257:86..653Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103257 87..653 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig103257.683.1prot_M-pyrifera_M_contig103257.683.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig103257 87..653 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig103257.683.1

>prot_M-pyrifera_M_contig103257.683.1 ID=prot_M-pyrifera_M_contig103257.683.1|Name=mRNA_M-pyrifera_M_contig103257.683.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=189bp
MNKPRPPKRDRTRRKLLDAGLEVMAERGEALTASDVVNAADVSNGTFYNH
FLDREDFIQALALESLEVITTGSADDTEGADPAWRFAVATTRVLEAATRE
PLWGRAILRLAECATPLHSGVRQHLLADLAEGHRTGRFTYGDDPTTVDLV
SGTIMASIRRMVSPDAPAEAAGNDAIPTVVSRLLEAIGV
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mRNA from alignment at M-pyrifera_M_contig103257:84..653+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig103257.683.1 ID=mRNA_M-pyrifera_M_contig103257.683.1|Name=mRNA_M-pyrifera_M_contig103257.683.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig103257:84..653+ (Macrocystis pyrifera P11B4 male)
ACGATGAACAAGCCGAGGCCACCGAAGCGAGATCGCACGCGTCGAAAGCT GCTCGATGCCGGGCTCGAGGTGATGGCAGAGCGCGGTGAAGCCCTCACCG CCTCCGACGTGGTGAATGCCGCCGATGTCTCGAACGGCACGTTCTACAAC CACTTCCTCGACCGAGAGGACTTCATCCAGGCCCTCGCCCTCGAGTCGCT CGAAGTGATCACCACAGGCAGCGCCGACGACACCGAGGGCGCCGATCCAG CCTGGCGCTTCGCTGTGGCGACGACACGGGTCCTCGAGGCAGCAACCCGA GAACCGCTCTGGGGCCGCGCCATCCTCCGGCTGGCGGAGTGTGCAACACC GCTGCACTCCGGTGTCCGACAACACCTCCTTGCCGACTTGGCCGAGGGCC ACCGCACCGGCCGCTTCACCTACGGCGACGACCCGACCACCGTCGATCTC GTCTCCGGCACGATCATGGCCTCAATTCGCCGAATGGTGTCCCCCGACGC TCCAGCCGAAGCCGCAGGGAACGATGCGATTCCCACGGTGGTCAGTCGGC TCCTGGAAGCGATCGGCGTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig103257:84..653+

>mRNA_M-pyrifera_M_contig103257.683.1 ID=mRNA_M-pyrifera_M_contig103257.683.1|Name=mRNA_M-pyrifera_M_contig103257.683.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1134bp|location=Sequence derived from alignment at M-pyrifera_M_contig103257:84..653+ (Macrocystis pyrifera P11B4 male)
ATGAACAAGCCGAGGCCACCGAAGCGAGATCGCACGCGTCGAAAGCTGCT
CGATGCCGGGCTCGAGGTGATGGCAGAGCGCGGTGAAGCCCTCACCGCCT
CCGACGTGGTGAATGCCGCCGATGTCTCGAACGGCACGTTCTACAACCAC
TTCCTCGACCGAGAGGACTTCATCCAGGCCCTCGCCCTCGAGTCGCTCGA
AGTGATCACCACAGGCAGCGCCGACGACACCGAGGGCGCCGATCCAGCCT
GGCGCTTCGCTGTGGCGACGACACGGGTCCTCGAGGCAGCAACCCGAGAA
CCGCTCTGGGGCCGCGCCATCCTCCGGCTGGCGGAGTGTGCAACACCGCT
GCACTCCGGTGTCCGACAACACCTCCTTGCCGACTTGGCCGAGGGCCACC
GCACCGGCCGCTTCACCTACGGCGACGACCCGACCACCGTCGATCTCGTC
TCCGGCACGATCATGGCCTCAATTCGCCGAATGGTGTCCCCCGACGCTCC
AGCCGAAGCCGCAGGGAACGATGCGATTCCCACGGTGGTCAGTCGGCTCC
TGGAAGCGATCGGCGTCATGAACAAGCCGAGGCCACCGAAGCGAGATCGC
ACGCGTCGAAAGCTGCTCGATGCCGGGCTCGAGGTGATGGCAGAGCGCGG
TGAAGCCCTCACCGCCTCCGACGTGGTGAATGCCGCCGATGTCTCGAACG
GCACGTTCTACAACCACTTCCTCGACCGAGAGGACTTCATCCAGGCCCTC
GCCCTCGAGTCGCTCGAAGTGATCACCACAGGCAGCGCCGACGACACCGA
GGGCGCCGATCCAGCCTGGCGCTTCGCTGTGGCGACGACACGGGTCCTCG
AGGCAGCAACCCGAGAACCGCTCTGGGGCCGCGCCATCCTCCGGCTGGCG
GAGTGTGCAACACCGCTGCACTCCGGTGTCCGACAACACCTCCTTGCCGA
CTTGGCCGAGGGCCACCGCACCGGCCGCTTCACCTACGGCGACGACCCGA
CCACCGTCGATCTCGTCTCCGGCACGATCATGGCCTCAATTCGCCGAATG
GTGTCCCCCGACGCTCCAGCCGAAGCCGCAGGGAACGATGCGATTCCCAC
GGTGGTCAGTCGGCTCCTGGAAGCGATCGGCGTC
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