mRNA_M-pyrifera_M_contig101785.375.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig101785.375.1
Unique NamemRNA_M-pyrifera_M_contig101785.375.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: F0Y671_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y671_AURAN)

HSP 1 Score: 89.4 bits (220), Expect = 3.140e-19
Identity = 52/123 (42.28%), Postives = 84/123 (68.29%), Query Frame = 1
Query:   55 ARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETE 420
            A+ P+ K + + +AM +LKRKKMYE Q +QL G   ++E+   AI+ +  T   V  MKA S+ LK +  ++D+D++ED++DD+ADM+ D +EV + +G  + +GD+V +ADLD E+A L+ E
Sbjct:   52 AKGPAAK-TLKQRAMAVLKRKKMYEAQRDQLAGQAFNVEQTSFAIDSVKDTLTTVDAMKAASKTLKTEFKKVDIDQIEDVQDDLADMMEDMNEVQDALGRSYGIGDDVDEADLDAELACLDDE 173          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A7S2AMY1_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2AMY1_9STRA)

HSP 1 Score: 84.3 bits (207), Expect = 4.000e-17
Identity = 46/111 (41.44%), Postives = 75/111 (67.57%), Query Frame = 1
Query:   91 KAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETE 420
            +AM+ LKRKKMYE+Q +Q+ G   ++E+   AI+ I  T+  V  MK  +  LKA+T ++++D +ED +DD+ DM+ D +E+ E+MG  + + DE+ + DLD E+A L+ E
Sbjct:   63 RAMDTLKRKKMYEQQRDQMAGQAFNIEQTSFAIDSIKDTQTTVAAMKVAASTLKAETKKLNIDSIEDTQDDLGDMMEDMNEINEMMGRSYGVPDEMDEEDLDAELACLDDE 173          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: E4WZN8_OIKDI (Uncharacterized protein n=1 Tax=Oikopleura dioica TaxID=34765 RepID=E4WZN8_OIKDI)

HSP 1 Score: 82.8 bits (203), Expect = 1.210e-16
Identity = 48/142 (33.80%), Postives = 93/142 (65.49%), Query Frame = 1
Query:   10 EVTAQFVQATKALKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETEGLAD 432
            ++ A+ V+    +K  R    K + + KAM +LK++KMYE Q EQ++    +M++    I+ +  T++ V  MKA ++ +K +  +I++D++ED++D++ DM+ DA+E+ E+MG ++ + D++ +ADL+ E+ AL  + LAD
Sbjct:   37 KLDAELVKYKNQMKTMRNGPGKNAVKQKAMRVLKQRKMYEGQREQISNQSFNMDQANFTIQSLKDTQDTVTAMKAGAKAMKKEYKKINIDKIEDLQDELEDMMLDANEIQEVMGRNYGISDDIDEADLEAELDALGDDFLAD 178          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A813A4N3_9DINO (Chmp5 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813A4N3_9DINO)

HSP 1 Score: 80.1 bits (196), Expect = 1.990e-15
Identity = 51/130 (39.23%), Postives = 78/130 (60.00%), Query Frame = 1
Query:   46 LKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETEGLAD 432
            LK  + P+ +R  RM+  N+L+R++M E+QL        SM+    AI+   M  + V   K+    L+ + A+I LDELEDM+DD+AD+LAD+ E+ ++MG  + L  E+ + DLD E+ ALE E   D
Sbjct:   55 LKRTKNPAGQRGVRMRMKNILQRRRMLEQQLNLTYNTTNSMQATAYAIDNASMIADQVSATKSAMVTLRKQHADISLDELEDMQDDMADLLADSAEMSDLMGRSYGLPGELDEGDLDAELEALEDELAGD 184          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: D8LTZ1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTZ1_ECTSI)

HSP 1 Score: 81.6 bits (200), Expect = 2.020e-15
Identity = 54/134 (40.30%), Postives = 84/134 (62.69%), Query Frame = 1
Query:   22 QFVQATKALKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETE 420
            + V+   ALK A+ P+   + + +AM  LKRKKMYE Q +Q+ G   ++E+ G AI+ I  T   V  MK  S+ LKA   +ID++++EDM DD+A      +E+ +IMG  +++GDE+ + DLD E+A L+ E
Sbjct:  115 ELVKYRNALKKAKGPTAT-NIKKRAMETLKRKKMYEGQRDQMAGQQFNIEQTGFAIDSIKDTATTVAAMKDASKTLKADIKKIDINKVEDMTDDMAXXXXXXNEINDIMGRSYNVGDELDEDDLDAELACLDDE 247          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A813B2T3_9DINO (Chmp5 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813B2T3_9DINO)

HSP 1 Score: 78.2 bits (191), Expect = 7.610e-15
Identity = 48/119 (40.34%), Postives = 78/119 (65.55%), Query Frame = 1
Query:   55 ARTP-SLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMA 405
            AR P S +   R +A   LKRKKMYE+Q   +     +ME+   A+E I  T + + +MK   + L+ +  ++D++ +ED++DD+AD++ADADE+ EIMG  + +G ++ +ADLDEE+A
Sbjct:   57 ARAPRSQQTMLRQRAAQALKRKKMYEQQRGTMMATQFNMEQTQFALENIKDTADHISSMKHARDTLQDQYKKLDINSVEDLQDDLADLMADADEINEIMGRSYGVGADIDEADLDEELA 175          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A7S0EZB4_9EUKA (Hypothetical protein n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0EZB4_9EUKA)

HSP 1 Score: 77.0 bits (188), Expect = 2.420e-14
Identity = 48/126 (38.10%), Postives = 78/126 (61.90%), Query Frame = 1
Query:   37 TKALKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMGDFSLGDEVGDADLDEEMAALE 414
            T+ LK  +    K+  + +AM +LK+KKMYE Q EQ      +M++V  A E +  T   VG MK  ++ LK +   ID++++EDM+DD++D+L  A+E+   MG     D++ +ADL+ E+AA+E
Sbjct:   59 TQQLKTMKPGPAKQQVQKRAMAILKQKKMYENQREQTRNQQFNMDQVMFAQESLAETANTVGAMKDANKALKKQFKAIDINQVEDMQDDMSDLLEQAEEIQSAMGRSYNTDDIDEADLEAELAAME 184          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A3R7GS33_9STRA (Uncharacterized protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7GS33_9STRA)

HSP 1 Score: 77.0 bits (188), Expect = 2.460e-14
Identity = 54/137 (39.42%), Postives = 84/137 (61.31%), Query Frame = 1
Query:   46 LKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETEGLADLESELEA 453
            +K  R P+L  + + +AM  LKRKKM+E Q ++LT    ++E+   AI+    T   V  MK+ +  LKA+T +ID+ ELED++DD+A        + EIMG  + +GDEV +++L+ E+A LE E   +  +ELEA
Sbjct:   51 MKKTRGPALN-AIKQRAMQTLKRKKMFEAQRDKLTAQSFNIEQAAFAIDTSRDTITTVAAMKSAAVQLKAETQKIDISELEDVQDDMAXXXXXXXXIQEIMGRSYGIGDEVDESELEAELAGLEEEWAEEGANELEA 186          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A812Y1R2_SYMMI (CHMP5 protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812Y1R2_SYMMI)

HSP 1 Score: 75.9 bits (185), Expect = 6.500e-14
Identity = 47/120 (39.17%), Postives = 75/120 (62.50%), Query Frame = 1
Query:   91 KAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETEGLADLESEL 447
            +A+ +LKRKKMYE+Q +QL G   ++++   A+E I  T   V  MK  ++ LK + A+IDL+E+ED+ DD+ADM  D +E+ E +G  +   D + + DL+ E+A L  E   +L  E+
Sbjct:   63 RAVEVLKRKKMYEQQRDQLCGQAFNLDQAAFAMETIKDTHTTVAAMKDAAKQLKVEHAKIDLNEIEDLNDDLADMFEDMNEISETLGRSYGTPDYIDEDDLEAELACLGDEFDEELTEEV 182          
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Match: A0A7S0QD78_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0QD78_9CRYP)

HSP 1 Score: 74.7 bits (182), Expect = 1.030e-13
Identity = 48/148 (32.43%), Postives = 88/148 (59.46%), Query Frame = 1
Query:    4 LREVTAQFVQATKALKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLTGMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRDDVADMLADADEVGEIMG-DFSLGDEVGDADLDEEMAALETEGLADLESE 444
            ++E+  Q       LK  R    +   + +A+ +LK+K+MYE Q +Q+ GMV +ME+   A E +         MKA++  LK +  +++L ELED+ DD++D+L D +E+ EI+   +++ D + +A+L++E+AAL+   L + E +
Sbjct:   37 IKELDKQLADFKVQLKTTRPGPAQTRIKQRALQVLKQKRMYESQQDQVRGMVWNMEQAKFATENVQGALFQAEAMKASTAELKNQMKKVNLSELEDVMDDMSDLLEDTNEMSEILSRSYAVDDNIDEAELEDELAALDELALDEAEED 184          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig101785.375.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
F0Y671_AURAN3.140e-1942.28Uncharacterized protein (Fragment) n=1 Tax=Aureoco... [more]
A0A7S2AMY1_9STRA4.000e-1741.44Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
E4WZN8_OIKDI1.210e-1633.80Uncharacterized protein n=1 Tax=Oikopleura dioica ... [more]
A0A813A4N3_9DINO1.990e-1539.23Chmp5 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=2... [more]
D8LTZ1_ECTSI2.020e-1540.30Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A813B2T3_9DINO7.610e-1540.34Chmp5 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=2... [more]
A0A7S0EZB4_9EUKA2.420e-1438.10Hypothetical protein n=1 Tax=Phaeocystis antarctic... [more]
A0A3R7GS33_9STRA2.460e-1439.42Uncharacterized protein n=3 Tax=Phytophthora kerno... [more]
A0A812Y1R2_SYMMI6.500e-1439.17CHMP5 protein n=1 Tax=Symbiodinium microadriaticum... [more]
A0A7S0QD78_9CRYP1.030e-1332.43Hypothetical protein (Fragment) n=1 Tax=Cryptomona... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig101785contigM-pyrifera_M_contig101785:138..590 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score87.4
Seed ortholog evalue6.1e-15
Seed eggNOG ortholog44056.XP_009035382.1
Preferred nameCHMP5
KEGG rclassRC00918,RC01781
KEGG koko:K01698,ko:K12198
KEGG ReactionR00036
KEGG Pathwayko00860,ko01100,ko01110,ko01120,ko04144,ko04217,map00860,map01100,map01110,map01120,map04144,map04217
KEGG ModuleM00121,M00412
Hectar predicted targeting categoryother localisation
GOsGO:0000070,GO:0000278,GO:0000280,GO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0000815,GO:0000819,GO:0000920,GO:0001919,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005768,GO:0005770,GO:0005771,GO:0005773,GO:0005774,GO:0005829,GO:0006810,GO:0006886,GO:0006996,GO:0006997,GO:0007032,GO:0007033,GO:0007034,GO:0007040,GO:0007041,GO:0007049,GO:0007059,GO:0007080,GO:0007088,GO:0007346,GO:0008104,GO:0008150,GO:0008333,GO:0009987,GO:0010008,GO:0010256,GO:0010564,GO:0010824,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016032,GO:0016043,GO:0016050,GO:0016192,GO:0016197,GO:0019058,GO:0019068,GO:0019222,GO:0022402,GO:0022411,GO:0022607,GO:0023051,GO:0030447,GO:0031090,GO:0031323,GO:0031410,GO:0031982,GO:0032509,GO:0032510,GO:0032511,GO:0032886,GO:0032984,GO:0032991,GO:0033036,GO:0033043,GO:0034613,GO:0036257,GO:0036258,GO:0036452,GO:0040007,GO:0042886,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044087,GO:0044403,GO:0044419,GO:0044422,GO:0044424,GO:0044425,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0045184,GO:0045324,GO:0046605,GO:0046755,GO:0046907,GO:0048284,GO:0048285,GO:0050000,GO:0050789,GO:0050794,GO:0051128,GO:0051179,GO:0051234,GO:0051276,GO:0051301,GO:0051303,GO:0051310,GO:0051493,GO:0051640,GO:0051641,GO:0051649,GO:0051656,GO:0051704,GO:0051726,GO:0051783,GO:0060236,GO:0060255,GO:0061024,GO:0061025,GO:0061763,GO:0065007,GO:0070507,GO:0070727,GO:0070925,GO:0071702,GO:0071705,GO:0071840,GO:0071985,GO:0080171,GO:0090169,GO:0090174,GO:0090224,GO:0097708,GO:0098588,GO:0098796,GO:0098805,GO:0098813,GO:0098852,GO:0140014,GO:1901673,GO:1902115,GO:1903047,GO:1904896,GO:1904903
EggNOG free text desc.vacuolar transport
EggNOG OGsKOG1655@1,KOG1655@2759
EC4.2.1.24
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04131,ko04147
Exons1
Model size453
Cds size453
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815517.5377388-CDS-M-pyrifera_M_contig101785:137..5901622815517.5377388-CDS-M-pyrifera_M_contig101785:137..590Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig101785 138..590 +
1692277446.3578413-CDS-M-pyrifera_M_contig101785:137..5901692277446.3578413-CDS-M-pyrifera_M_contig101785:137..590Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig101785 138..590 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig101785.375.1prot_M-pyrifera_M_contig101785.375.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig101785 138..590 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig101785.375.1

>prot_M-pyrifera_M_contig101785.375.1 ID=prot_M-pyrifera_M_contig101785.375.1|Name=mRNA_M-pyrifera_M_contig101785.375.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=151bp
QLREVTAQFVQATKALKAARTPSLKRSARMKAMNLLKRKKMYEKQLEQLT
GMVMSMERVGGAIEQIDMTREMVGTMKATSEVLKAKTAEIDLDELEDMRD
DVADMLADADEVGEIMGDFSLGDEVGDADLDEEMAALETEGLADLESELE
A
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mRNA from alignment at M-pyrifera_M_contig101785:138..590+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig101785.375.1 ID=mRNA_M-pyrifera_M_contig101785.375.1|Name=mRNA_M-pyrifera_M_contig101785.375.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=453bp|location=Sequence derived from alignment at M-pyrifera_M_contig101785:138..590+ (Macrocystis pyrifera P11B4 male)
CAGCTCCGTGAGGTGACGGCTCAGTTTGTGCAAGCGACGAAGGCGTTGAA GGCAGCTCGTACTCCTAGTTTGAAGCGGAGCGCGCGTATGAAGGCAATGA ATTTGCTGAAGCGGAAGAAGATGTACGAGAAGCAGCTGGAGCAGCTGACG GGTATGGTGATGTCGATGGAGCGTGTGGGGGGTGCGATCGAGCAGATCGA CATGACGCGGGAGATGGTGGGCACGATGAAGGCGACTTCTGAGGTGCTCA AGGCGAAGACTGCGGAAATCGACCTGGACGAGTTGGAGGATATGCGGGAT GACGTGGCAGATATGTTGGCGGATGCCGACGAGGTGGGCGAAATCATGGG CGATTTTTCGCTCGGGGATGAAGTTGGGGACGCAGATTTGGACGAGGAGA TGGCAGCGCTGGAGACTGAAGGGCTTGCGGATCTTGAAAGTGAACTCGAA GCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig101785:138..590+

>mRNA_M-pyrifera_M_contig101785.375.1 ID=mRNA_M-pyrifera_M_contig101785.375.1|Name=mRNA_M-pyrifera_M_contig101785.375.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=906bp|location=Sequence derived from alignment at M-pyrifera_M_contig101785:138..590+ (Macrocystis pyrifera P11B4 male)
CAGCTCCGTGAGGTGACGGCTCAGTTTGTGCAAGCGACGAAGGCGTTGAA
GGCAGCTCGTACTCCTAGTTTGAAGCGGAGCGCGCGTATGAAGGCAATGA
ATTTGCTGAAGCGGAAGAAGATGTACGAGAAGCAGCTGGAGCAGCTGACG
GGTATGGTGATGTCGATGGAGCGTGTGGGGGGTGCGATCGAGCAGATCGA
CATGACGCGGGAGATGGTGGGCACGATGAAGGCGACTTCTGAGGTGCTCA
AGGCGAAGACTGCGGAAATCGACCTGGACGAGTTGGAGGATATGCGGGAT
GACGTGGCAGATATGTTGGCGGATGCCGACGAGGTGGGCGAAATCATGGG
CGATTTTTCGCTCGGGGATGAAGTTGGGGACGCAGATTTGGACGAGGAGA
TGGCAGCGCTGGAGACTGAAGGGCTTGCGGATCTTGAAAGTGAACTCGAA
GCCCAGCTCCGTGAGGTGACGGCTCAGTTTGTGCAAGCGACGAAGGCGTT
GAAGGCAGCTCGTACTCCTAGTTTGAAGCGGAGCGCGCGTATGAAGGCAA
TGAATTTGCTGAAGCGGAAGAAGATGTACGAGAAGCAGCTGGAGCAGCTG
ACGGGTATGGTGATGTCGATGGAGCGTGTGGGGGGTGCGATCGAGCAGAT
CGACATGACGCGGGAGATGGTGGGCACGATGAAGGCGACTTCTGAGGTGC
TCAAGGCGAAGACTGCGGAAATCGACCTGGACGAGTTGGAGGATATGCGG
GATGACGTGGCAGATATGTTGGCGGATGCCGACGAGGTGGGCGAAATCAT
GGGCGATTTTTCGCTCGGGGATGAAGTTGGGGACGCAGATTTGGACGAGG
AGATGGCAGCGCTGGAGACTGAAGGGCTTGCGGATCTTGAAAGTGAACTC
GAAGCC
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