mRNA_M-pyrifera_M_contig100930.206.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100930.206.1
Unique NamemRNA_M-pyrifera_M_contig100930.206.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A7V9A655_9BACT (Mannosylfructose-phosphate phosphatase n=2 Tax=Bremerella alba TaxID=980252 RepID=A0A7V9A655_9BACT)

HSP 1 Score: 330 bits (846), Expect = 5.520e-113
Identity = 164/165 (99.39%), Postives = 165/165 (100.00%), Query Frame = 1
Query:    1 FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLD+LSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL
Sbjct:  100 FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDHLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 264          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A518CA86_9BACT (Mannosylfructose-phosphate phosphatase n=2 Tax=Bremerella volcania TaxID=2527984 RepID=A0A518CA86_9BACT)

HSP 1 Score: 290 bits (742), Expect = 4.390e-97
Identity = 139/164 (84.76%), Postives = 156/164 (95.12%), Query Frame = 1
Query:    4 STGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            ST +LREEFASLE+LR+QEEEKQG FKLSFYCDAK+LH +HDAVLD L+HR LPYSLISS+DPF GDGLIDLLPTDVSKA+ALQWWT+HAG+DN++LVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVH HYE+RMQ+DR+Y+AEK ATSGVLAGCRHFGL+
Sbjct:  106 STSRLREEFASLESLRVQEEEKQGPFKLSFYCDAKRLHEVHDAVLDQLTHRRLPYSLISSVDPFNGDGLIDLLPTDVSKAHALQWWTDHAGMDNRQLVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHQHYEARMQLDRLYLAEKTATSGVLAGCRHFGLI 269          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A2S8EZP2_9BACT (S6PP domain-containing protein n=1 Tax=Blastopirellula marina TaxID=124 RepID=A0A2S8EZP2_9BACT)

HSP 1 Score: 288 bits (737), Expect = 2.140e-96
Identity = 141/164 (85.98%), Postives = 152/164 (92.68%), Query Frame = 1
Query:    4 STGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            +T +LREEFASL+ LRLQEEEKQG FKLSFYCDAK+L A+HD VL  L+HR LPYSLISS+DPF GDGLIDLLPTDVSKAYALQWWTEHAGLDN+ELVFSGDSGNDLAAMTAGYRTIVVGNAS ELMETVH HYESR+QVDRVY+AEK ATSGVLAGCRHFGL+
Sbjct:  101 TTSRLREEFASLDMLRLQEEEKQGPFKLSFYCDAKQLRAVHDQVLHQLTHRALPYSLISSVDPFNGDGLIDLLPTDVSKAYALQWWTEHAGLDNRELVFSGDSGNDLAAMTAGYRTIVVGNASPELMETVHQHYESRLQVDRVYLAEKSATSGVLAGCRHFGLI 264          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: UPI001CCEFE47 (HAD family hydrolase n=1 Tax=Bremerella volcania TaxID=2527984 RepID=UPI001CCEFE47)

HSP 1 Score: 280 bits (715), Expect = 4.900e-93
Identity = 135/165 (81.82%), Postives = 150/165 (90.91%), Query Frame = 1
Query:    1 FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            FS G++RE+FA  + LRLQEEEKQG+FKLSFYCDAK+L  IHD VLD ++HR LPYS+ISS+DPFTGDGLIDLLPTDVSKAYALQWWT+H GLDN+ELVFSGDSGNDLAAMTAGYRTIVVGNAS +LMETVH HYES+ QVDRVYIA K ATSGVLAGCRHFGL+
Sbjct:  100 FSPGKIREQFAENQALRLQEEEKQGKFKLSFYCDAKQLRKIHDDVLDTITHRELPYSVISSVDPFTGDGLIDLLPTDVSKAYALQWWTDHTGLDNKELVFSGDSGNDLAAMTAGYRTIVVGNASGDLMETVHQHYESKRQVDRVYIARKHATSGVLAGCRHFGLI 264          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A368KXC4_9BACT (HAD-IIB family hydrolase n=1 Tax=Blastopirellula cremea TaxID=1031537 RepID=A0A368KXC4_9BACT)

HSP 1 Score: 220 bits (561), Expect = 1.120e-69
Identity = 104/165 (63.03%), Postives = 134/165 (81.21%), Query Frame = 1
Query:    1 FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            +ST  +R++F++ + LRLQE+EKQG FKLSFYCDA ++ ++   +   L+   +PY++I S+DPF GDGLIDLLP DVSKA+ALQWWT+H GL N+E VF+GDSGNDLAA+TAGYRTIVVGNA   L ETV  H+E+  Q+DR+Y+AEK ATSGVLAGC+HFGL+
Sbjct:   99 YSTKVVRQKFSAWDALRLQEQEKQGTFKLSFYCDATQVKSLVQMLRQALAEHAMPYNVIGSVDPFNGDGLIDLLPADVSKAHALQWWTKHTGLANEETVFAGDSGNDLAALTAGYRTIVVGNADRALAETVRTHFENCQQLDRLYLAEKTATSGVLAGCQHFGLV 263          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A2S8FE01_9BACT (S6PP domain-containing protein n=2 Tax=Pirellulaceae TaxID=2691357 RepID=A0A2S8FE01_9BACT)

HSP 1 Score: 216 bits (551), Expect = 5.160e-68
Identity = 104/165 (63.03%), Postives = 128/165 (77.58%), Query Frame = 1
Query:    1 FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            +ST  +RE+F++   LR QEEEKQG FKLSFYCDAK++  +   +   L    +PY++I S+DPF GDGLIDLLP +VSKA+ALQWWT H G+ N+E VF+GDSGNDLAAMTAGYRTIVVGNA   L  TV  H+E   Q++R+Y+AEK ATSGVLAGC+HFGLL
Sbjct:  110 YSTKAVREKFSTWNQLRPQEEEKQGTFKLSFYCDAKEVETLVANIRSTLGEHDIPYNVIGSVDPFNGDGLIDLLPANVSKAHALQWWTRHTGMANEEAVFAGDSGNDLAAMTAGYRTIVVGNADRALANTVRAHFEECQQLERLYLAEKTATSGVLAGCQHFGLL 274          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A517NYH6_9BACT (Mannosylfructose-phosphate phosphatase n=1 Tax=Planctomycetes bacterium K23_9 TaxID=1930275 RepID=A0A517NYH6_9BACT)

HSP 1 Score: 177 bits (448), Expect = 1.220e-52
Identity = 91/160 (56.88%), Postives = 111/160 (69.38%), Query Frame = 1
Query:    7 TGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHF 486
            T  L E F+ +  LR QEE KQ +FKLSFY DAK L      + + +S   LPY +I+S+DPFTGDGLIDLLP  VSKA AL WW +H G   Q +VFSGDSGNDLAA+TAGYR+IVVGNASA L+E V   + S    +R+++A+  ATSGVL G  HF
Sbjct:   94 TAALLEPFSEIAGLRKQEESKQSEFKLSFYADAKHLDKTAGLLQNLVSEMKLPYEMIASVDPFTGDGLIDLLPRGVSKASALDWWAKHVGCHRQSIVFSGDSGNDLAALTAGYRSIVVGNASASLIEKVQQKHRSSAWDNRLHVAQASATSGVLEGLEHF 253          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A2E2C0M3_9PLAN (Malto-oligosyltrehalose trehalohydrolase n=3 Tax=Planctomycetaceae TaxID=126 RepID=A0A2E2C0M3_9PLAN)

HSP 1 Score: 186 bits (473), Expect = 6.930e-52
Identity = 91/159 (57.23%), Postives = 122/159 (76.73%), Query Frame = 1
Query:   16 LREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGL 492
            LRE+ A+++ LRLQE  KQG+FKLS+Y DA +L ++ D + + L+    PYS+I+S+DPF GDGLIDLLP  VSKA AL+WWTE+ G D + +VF+GDSGNDLAA+TAGYRTI+VGNA  +L + V+  +++    +R+Y+AE  ATSGVL GCR FGL
Sbjct:  119 LREQLAAIDGLRLQESFKQGRFKLSYYADAAQLESLVDRIQEILTQTDAPYSIINSVDPFNGDGLIDLLPATVSKALALEWWTENNGYDPRNIVFAGDSGNDLAALTAGYRTILVGNADRQLAQRVYNLHQTSGWKNRLYLAEGTATSGVLEGCRWFGL 277          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A517M3J2_9BACT (Mannosylfructose-phosphate phosphatase n=1 Tax=Rosistilla ulvae TaxID=1930277 RepID=A0A517M3J2_9BACT)

HSP 1 Score: 174 bits (441), Expect = 1.850e-51
Identity = 86/160 (53.75%), Postives = 111/160 (69.38%), Query Frame = 1
Query:   16 LREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            +R+   S+  LRLQE EKQG+FKLS+Y D   L A+   + D +    LP+S+I+S+DPF GDGLIDLLP   SKA+AL+WW EH G    EL+F+GDSGNDLAA+TAGYR IVVGNA A ++E     +      DR+ + ++ ATSGVL GCR FGL+
Sbjct:  108 VRQTLQSIAGLRLQEPEKQGRFKLSYYADQADLSALAQQIADRIGE--LPFSMITSVDPFNGDGLIDLLPKGTSKAFALRWWAEHVGRSETELIFAGDSGNDLAALTAGYRAIVVGNADASVVEATRSAHRESGWTDRLVVTDQKATSGVLEGCRFFGLI 265          
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Match: A0A518JWG4_9BACT (Mannosylfructose-phosphate phosphatase n=1 Tax=Rosistilla carotiformis TaxID=2528017 RepID=A0A518JWG4_9BACT)

HSP 1 Score: 172 bits (437), Expect = 7.440e-51
Identity = 87/154 (56.49%), Postives = 110/154 (71.43%), Query Frame = 1
Query:   34 SLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLSHRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVFSGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKFATSGVLAGCRHFGLL 495
            S++ LRLQE EKQG+FKLS+Y D   L  +   V   +S   LPYS+ISS+DPF GDGLIDLLP   SKA+ALQWWTEH G +  +L+F+GDSGNDLAA+TAGYR IVVGNAS  +++     +      DR+++A + ATSGVL GCR  GL+
Sbjct:  114 SIDGLRLQESEKQGRFKLSYYADQAVLPTVTQQVGARISE--LPYSMISSVDPFNGDGLIDLLPRGTSKAFALQWWTEHVGREATDLIFAGDSGNDLAALTAGYRAIVVGNASDAVVQAARAAHRDAGWTDRLHVAREVATSGVLEGCRFHGLI 265          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100930.206.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7V9A655_9BACT5.520e-11399.39Mannosylfructose-phosphate phosphatase n=2 Tax=Bre... [more]
A0A518CA86_9BACT4.390e-9784.76Mannosylfructose-phosphate phosphatase n=2 Tax=Bre... [more]
A0A2S8EZP2_9BACT2.140e-9685.98S6PP domain-containing protein n=1 Tax=Blastopirel... [more]
UPI001CCEFE474.900e-9381.82HAD family hydrolase n=1 Tax=Bremerella volcania T... [more]
A0A368KXC4_9BACT1.120e-6963.03HAD-IIB family hydrolase n=1 Tax=Blastopirellula c... [more]
A0A2S8FE01_9BACT5.160e-6863.03S6PP domain-containing protein n=2 Tax=Pirellulace... [more]
A0A517NYH6_9BACT1.220e-5256.88Mannosylfructose-phosphate phosphatase n=1 Tax=Pla... [more]
A0A2E2C0M3_9PLAN6.930e-5257.23Malto-oligosyltrehalose trehalohydrolase n=3 Tax=P... [more]
A0A517M3J2_9BACT1.850e-5153.75Mannosylfructose-phosphate phosphatase n=1 Tax=Ros... [more]
A0A518JWG4_9BACT7.440e-5156.49Mannosylfructose-phosphate phosphatase n=1 Tax=Ros... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100930contigM-pyrifera_M_contig100930:3..500 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score185.3
Seed ortholog evalue2.4e-44
Seed eggNOG ortholog344747.PM8797T_31008
Preferred nametreZ
KEGG rclassRC00049
KEGG koko:K00700,ko:K01176,ko:K01214,ko:K01236
KEGG ReactionR02108,R02110,R02112,R09995,R11256,R11261,R11262
KEGG Pathwayko00500,ko01100,ko01110,ko04973,map00500,map01100,map01110,map04973
KEGG ModuleM00565
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Alpha amylase, catalytic domain
EggNOG OGs2IXPW@203682,COG0296@1,COG0296@2,COG0561@1,COG0561@2
EC2.4.1.18,3.2.1.1,3.2.1.141,3.2.1.68
COG Functional cat.G
CAZyCBM48,GH13
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Exons1
Model size498
Cds size498
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815506.9261923-CDS-M-pyrifera_M_contig100930:2..5001622815506.9261923-CDS-M-pyrifera_M_contig100930:2..500Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100930 3..500 +
1692277442.4107287-CDS-M-pyrifera_M_contig100930:2..5001692277442.4107287-CDS-M-pyrifera_M_contig100930:2..500Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100930 3..500 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100930.206.1prot_M-pyrifera_M_contig100930.206.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100930 3..500 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100930.206.1

>prot_M-pyrifera_M_contig100930.206.1 ID=prot_M-pyrifera_M_contig100930.206.1|Name=mRNA_M-pyrifera_M_contig100930.206.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=166bp
FSTGQLREEFASLETLRLQEEEKQGQFKLSFYCDAKKLHAIHDAVLDYLS
HRGLPYSLISSIDPFTGDGLIDLLPTDVSKAYALQWWTEHAGLDNQELVF
SGDSGNDLAAMTAGYRTIVVGNASAELMETVHGHYESRMQVDRVYIAEKF
ATSGVLAGCRHFGLL*
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mRNA from alignment at M-pyrifera_M_contig100930:3..500+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100930.206.1 ID=mRNA_M-pyrifera_M_contig100930.206.1|Name=mRNA_M-pyrifera_M_contig100930.206.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=498bp|location=Sequence derived from alignment at M-pyrifera_M_contig100930:3..500+ (Macrocystis pyrifera P11B4 male)
TTTTCAACTGGGCAATTGCGAGAAGAGTTCGCCTCGCTAGAGACGCTACG ACTGCAAGAGGAAGAGAAGCAGGGGCAATTCAAACTCAGTTTCTATTGCG ACGCGAAAAAGCTTCACGCCATCCACGACGCGGTCTTAGATTATTTGTCC CATCGTGGTCTGCCGTACAGCTTGATCAGCAGTATCGATCCATTCACCGG CGACGGCCTGATCGACCTTTTACCAACCGACGTCTCGAAGGCCTACGCGC TACAGTGGTGGACCGAGCATGCCGGGCTAGACAATCAAGAGCTCGTCTTC TCTGGCGACTCTGGCAACGACTTGGCAGCCATGACCGCCGGCTATCGGAC GATTGTCGTCGGCAACGCTTCTGCAGAACTCATGGAAACGGTCCACGGAC ATTACGAAAGCCGCATGCAAGTCGACCGGGTCTACATTGCCGAGAAGTTC GCCACCAGCGGAGTCCTCGCTGGCTGCCGACACTTTGGACTGCTTTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100930:3..500+

>mRNA_M-pyrifera_M_contig100930.206.1 ID=mRNA_M-pyrifera_M_contig100930.206.1|Name=mRNA_M-pyrifera_M_contig100930.206.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=996bp|location=Sequence derived from alignment at M-pyrifera_M_contig100930:3..500+ (Macrocystis pyrifera P11B4 male)
TTTTCAACTGGGCAATTGCGAGAAGAGTTCGCCTCGCTAGAGACGCTACG
ACTGCAAGAGGAAGAGAAGCAGGGGCAATTCAAACTCAGTTTCTATTGCG
ACGCGAAAAAGCTTCACGCCATCCACGACGCGGTCTTAGATTATTTGTCC
CATCGTGGTCTGCCGTACAGCTTGATCAGCAGTATCGATCCATTCACCGG
CGACGGCCTGATCGACCTTTTACCAACCGACGTCTCGAAGGCCTACGCGC
TACAGTGGTGGACCGAGCATGCCGGGCTAGACAATCAAGAGCTCGTCTTC
TCTGGCGACTCTGGCAACGACTTGGCAGCCATGACCGCCGGCTATCGGAC
GATTGTCGTCGGCAACGCTTCTGCAGAACTCATGGAAACGGTCCACGGAC
ATTACGAAAGCCGCATGCAAGTCGACCGGGTCTACATTGCCGAGAAGTTC
GCCACCAGCGGAGTCCTCGCTGGCTGCCGACACTTTGGACTGCTTTAGTT
TTCAACTGGGCAATTGCGAGAAGAGTTCGCCTCGCTAGAGACGCTACGAC
TGCAAGAGGAAGAGAAGCAGGGGCAATTCAAACTCAGTTTCTATTGCGAC
GCGAAAAAGCTTCACGCCATCCACGACGCGGTCTTAGATTATTTGTCCCA
TCGTGGTCTGCCGTACAGCTTGATCAGCAGTATCGATCCATTCACCGGCG
ACGGCCTGATCGACCTTTTACCAACCGACGTCTCGAAGGCCTACGCGCTA
CAGTGGTGGACCGAGCATGCCGGGCTAGACAATCAAGAGCTCGTCTTCTC
TGGCGACTCTGGCAACGACTTGGCAGCCATGACCGCCGGCTATCGGACGA
TTGTCGTCGGCAACGCTTCTGCAGAACTCATGGAAACGGTCCACGGACAT
TACGAAAGCCGCATGCAAGTCGACCGGGTCTACATTGCCGAGAAGTTCGC
CACCAGCGGAGTCCTCGCTGGCTGCCGACACTTTGGACTGCTTTAG
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