mRNA_M-pyrifera_M_contig100512.107.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100512.107.1
Unique NamemRNA_M-pyrifera_M_contig100512.107.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A8H7U8Y2_MORIS (Thioredoxin-like_fold domain-containing protein n=1 Tax=Mortierella isabellina TaxID=91625 RepID=A0A8H7U8Y2_MORIS)

HSP 1 Score: 140 bits (354), Expect = 1.600e-39
Identity = 70/139 (50.36%), Postives = 91/139 (65.47%), Query Frame = 1
Query:   10 MALPPAHQSHRLGSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKLKV 426
            MAL P    HRLGS  A   +E++ D  CPFS K  +   K+++P+LE+EH   VQVIFRQQIQ WHP +T++HEAA+AVE +   KFW+Y  ALFE Q  +FD A    SRN+ Y +LADLAA + V  + VL  L +
Sbjct:    1 MALAPQFIGHRLGSVAAKHTLEIYLDYTCPFSAKIHKKIRKDVVPYLEKEHPEQVQVIFRQQIQPWHPTSTLLHEAAIAVERIDHKKFWEYSDALFEHQTEYFDEAVYTKSRNEIYGKLADLAASINVPGDKVLSLLTI 139          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A261Y5Q7_9FUNG (Thioredoxin-like_fold domain-containing protein n=1 Tax=Bifiguratus adelaidae TaxID=1938954 RepID=A0A261Y5Q7_9FUNG)

HSP 1 Score: 134 bits (336), Expect = 8.400e-37
Identity = 68/141 (48.23%), Postives = 95/141 (67.38%), Query Frame = 1
Query:   10 MALPPAHQSHRLGSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLA-AKVGVSKEAVLEKLKVI 429
            MAL P    HR+G+A AP  +EV+ D  CPFS K +R   + ++P++E+ + G VQ IFRQQ+Q WHP +TM+HEAA+AVEA+  +KF  +  ALFE+Q+ +FD    + SR+  Y QLADL  A VG+S E  LE + V+
Sbjct:    1 MALAPQFIGHRIGAATAPHTLEVYLDYVCPFSAKIYRKLREIILPYIEQHYPGQVQFIFRQQVQPWHPTSTMVHEAAIAVEAIDASKFMPFSDALFEKQKDYFDEFVYNKSRHAIYGQLADLVQASVGISGEKFLELVSVV 141          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A7S4I469_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4I469_9EUKA)

HSP 1 Score: 127 bits (320), Expect = 2.280e-35
Identity = 58/121 (47.93%), Postives = 86/121 (71.07%), Query Frame = 1
Query:   37 HRLGSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSK 399
            H+LGSA A  V+E++ D CCPFS K++ T  K ++P  EE H GV+Q + R Q+Q WHPQ+T+ HEAALAVE++  +KF+++ + +F+ QE+F D    + +R + Y  LADLA++VGV K
Sbjct:    2 HKLGSADARYVVELWLDYCCPFSRKAYDTMVKRVIPHFEENHPGVLQFVLRHQVQPWHPQSTLTHEAALAVESIDSSKFFEFSSLVFDNQESFNDENTFNKTRQELYNTLADLASRVGVDK 122          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A167XK72_9HYPO (Thioredoxin-like fold protein n=1 Tax=Moelleriella libera RCEF 2490 TaxID=1081109 RepID=A0A167XK72_9HYPO)

HSP 1 Score: 128 bits (321), Expect = 2.310e-34
Identity = 69/150 (46.00%), Postives = 96/150 (64.00%), Query Frame = 1
Query:   10 MALPPAHQSHRL------GSAGAPV---VIEVFWDLCCPFSTKSFRTFTKELMPWLEE--EHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKLKV 426
            MA+PP    H+L       ++ AP      E++ D CCPFS K FR  TKE++P + E  E    + +IFRQQ+Q WHP +T+MHEA LAV  +A  KFWD+ A LFEEQ+ +FD + ++ +RN  Y +LA +AAKVGV +  VL+ LK+
Sbjct:    1 MAVPPKFAGHKLEFSPPSSASAAPHSTHTFELYLDYCCPFSAKIFRNLTKEVIPAIRENTEWSSKLTLIFRQQVQPWHPSSTLMHEAGLAVLRLAPEKFWDFSAQLFEEQKDYFDVSVVNETRNATYGRLAKVAAKVGVDESEVLKLLKI 150          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A084QH19_STAC4 (Thioredoxin-like_fold domain-containing protein n=3 Tax=Stachybotrys TaxID=74721 RepID=A0A084QH19_STAC4)

HSP 1 Score: 125 bits (315), Expect = 2.000e-33
Identity = 69/153 (45.10%), Postives = 92/153 (60.13%), Query Frame = 1
Query:   10 MALPPAHQSHRL------------GSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGV--VQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKLKV 426
            MALPP    H+L            G+   P  +E+F D  CPFS K FRT T+ + P +         + V+FRQQ+Q WHP +T+MHEAALAV  +A AKFWD+ AALF EQ +FFD   ++ +RNQ Y +LA LAA+VGV +  V   L++
Sbjct:    1 MALPPKFAGHKLQFTSSPSSTAPSGAPATPHTLEIFLDYACPFSAKLFRTVTESVAPEIRARDAWASNLVVVFRQQVQPWHPSSTLMHEAALAVLRLAPAKFWDFSAALFREQTSFFDVNVVNETRNQTYKRLAKLAAEVGVDEAEVYGMLEI 153          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A443I662_BYSSP (Thioredoxin-like protein n=1 Tax=Byssochlamys spectabilis TaxID=264951 RepID=A0A443I662_BYSSP)

HSP 1 Score: 125 bits (314), Expect = 2.290e-33
Identity = 66/142 (46.48%), Postives = 90/142 (63.38%), Query Frame = 1
Query:   10 MALPPAHQSHRLGSAGAPV-----VIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKL 420
            MALPP +   +L +A A V      +E++ D  CPFS K F TF   + P + E++K  +QVIFRQQIQ WHP +T+ HEAA AV  VA  KFWD+ AALF+ Q+ FFD   ++ +RN+ Y +LA +A  +GV +  VL  L
Sbjct:    1 MALPPKYAGQKLFAAAATVQQAHHTLELYLDYVCPFSAKLFNTFYTSVRPVVVEKYKSKLQVIFRQQIQPWHPSSTLTHEAAAAVLKVAPEKFWDFSAALFKHQKDFFDENVVNETRNKTYGRLAKIAGSIGVDESEVLSLL 142          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A1Y1XDF6_9FUNG (Thioredoxin-like_fold domain-containing protein n=1 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1XDF6_9FUNG)

HSP 1 Score: 125 bits (314), Expect = 2.290e-33
Identity = 61/131 (46.56%), Postives = 84/131 (64.12%), Query Frame = 1
Query:   28 HQSHRLGSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKL 420
            +  HR+GS GA   +E + D  CPFS + F T TKE++P++++E  G VQ I R Q+Q WHPQ+T++HEA+LA E V   KF  Y   LF+ Q  +FD AC +LSR Q Y  LA+ AA +G+ +E  L  L
Sbjct:   17 YSGHRVGSLGAAHTVEYYVDFTCPFSRRIFNTLTKEIIPYVQKEFGGSVQFILRHQVQPWHPQSTLLHEASLAAEKVEPKKFMAYAEKLFDVQRDYFDEACHNLSRTQIYHSLAEHAAFLGIDREEFLSYL 147          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A5C6GEK0_9HYPO (Uncharacterized protein n=2 Tax=Metarhizium rileyi TaxID=1649241 RepID=A0A5C6GEK0_9HYPO)

HSP 1 Score: 123 bits (309), Expect = 1.510e-32
Identity = 66/151 (43.71%), Postives = 95/151 (62.91%), Query Frame = 1
Query:   10 MALPPAHQSHRL-------GSAGAPV---VIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGV--VQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKLKV 426
            MA+PP    H+L        ++ AP      E++ D CCPFS K FRT TKE++P + +       + +IFRQQ+Q WHP +T+MHEA LAV  +A  KFWD+ + LFE+Q+AFFD + ++ +RN  Y +LA +AAKVGV +  V   L++
Sbjct:    1 MAVPPKFAGHKLEFAPSAPSTSSAPHSTHTFELYVDYCCPFSAKLFRTLTKEVIPAIRDNKSWASSLTLIFRQQVQPWHPSSTLMHEAGLAVLHLAPDKFWDFSSQLFEQQDAFFDVSVVNETRNATYRRLAKIAAKVGVDEAHVYRSLEI 151          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: B6QMH5_TALMQ (Thioredoxin-like_fold domain-containing protein n=7 Tax=Talaromyces sect. Talaromyces TaxID=2752537 RepID=B6QMH5_TALMQ)

HSP 1 Score: 122 bits (305), Expect = 4.530e-32
Identity = 64/140 (45.71%), Postives = 88/140 (62.86%), Query Frame = 1
Query:   10 MALPPAHQSHRLGSAG---APVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKL 420
            M+LPP  +  +L +A    +P  IE++ D  CPFS K F TF   + P + E++   VQVIFRQQIQ WHP +T++HEA  AV  VA  KFWD+   LF+EQ+ +FD   ++  RN  Y +LA LAA VGV ++ V + L
Sbjct:    1 MSLPPKFRGQKLAAANIGSSPHTIELYLDYVCPFSAKLFNTFYTSVKPLITEKYGSKVQVIFRQQIQPWHPSSTLVHEAGAAVLKVAPEKFWDFSQVLFKEQKEYFDEKVVNEIRNDTYKRLAALAATVGVDEKKVYDLL 140          
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Match: A0A4P9ZXS9_9FUNG (Thioredoxin-like protein n=1 Tax=Dimargaris cristalligena TaxID=215637 RepID=A0A4P9ZXS9_9FUNG)

HSP 1 Score: 122 bits (305), Expect = 5.880e-32
Identity = 62/134 (46.27%), Postives = 90/134 (67.16%), Query Frame = 1
Query:   28 HQSHRLGSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEEHKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQEAFFDPACMDLSRNQCYAQLADLAAKV-GVSKEAVLEKLKV 426
            +Q HRLG+  APVV+E + D  CPFS K F+TFT+ + P+++  +   VQ IFR Q+Q WHPQ++++HEAALAVEAV   +F+ + AALF+ Q  FFD A    SR++    LA  A +V G+  + V+++L V
Sbjct:    8 NQGHRLGAENAPVVVEAYLDYVCPFSAKFFKTFTERVFPYVQNTYPDQVQFIFRHQVQPWHPQSSILHEAALAVEAVDPDQFFPFSAALFDRQANFFDAAVHHQSRHELNTALAQYAHEVTGLKSDQVVKQLNV 141          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100512.107.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8H7U8Y2_MORIS1.600e-3950.36Thioredoxin-like_fold domain-containing protein n=... [more]
A0A261Y5Q7_9FUNG8.400e-3748.23Thioredoxin-like_fold domain-containing protein n=... [more]
A0A7S4I469_9EUKA2.280e-3547.93Hypothetical protein (Fragment) n=1 Tax=Vannella s... [more]
A0A167XK72_9HYPO2.310e-3446.00Thioredoxin-like fold protein n=1 Tax=Moelleriella... [more]
A0A084QH19_STAC42.000e-3345.10Thioredoxin-like_fold domain-containing protein n=... [more]
A0A443I662_BYSSP2.290e-3346.48Thioredoxin-like protein n=1 Tax=Byssochlamys spec... [more]
A0A1Y1XDF6_9FUNG2.290e-3346.56Thioredoxin-like_fold domain-containing protein n=... [more]
A0A5C6GEK0_9HYPO1.510e-3243.71Uncharacterized protein n=2 Tax=Metarhizium rileyi... [more]
B6QMH5_TALMQ4.530e-3245.71Thioredoxin-like_fold domain-containing protein n=... [more]
A0A4P9ZXS9_9FUNG5.880e-3246.27Thioredoxin-like protein n=1 Tax=Dimargaris crista... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100512contigM-pyrifera_M_contig100512:19..664 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score130.6
Seed ortholog evalue5.9e-28
Seed eggNOG ortholog5059.CADAFLAP00013333
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Thioredoxin
EggNOG OGs20FKM@147545,2B2AB@1,2S0CB@2759,39VNM@33154,3NXUU@4751,3QU4Q@4890,3S7DS@5042
COG Functional cat.S
Best tax levelEurotiales
Best eggNOG OGNA|NA|NA
Exons3
Model size432
Cds size423
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100512.107.1prot_M-pyrifera_M_contig100512.107.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100512 28..664 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815501.4162157-UTR-M-pyrifera_M_contig100512:18..271622815501.4162157-UTR-M-pyrifera_M_contig100512:18..27Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig100512 19..27 +
1692277439.9727936-UTR-M-pyrifera_M_contig100512:18..271692277439.9727936-UTR-M-pyrifera_M_contig100512:18..27Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig100512 19..27 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815501.4370072-CDS-M-pyrifera_M_contig100512:27..2501622815501.4370072-CDS-M-pyrifera_M_contig100512:27..250Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100512 28..250 +
1692277439.9821236-CDS-M-pyrifera_M_contig100512:27..2501692277439.9821236-CDS-M-pyrifera_M_contig100512:27..250Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100512 28..250 +
1622815501.4486244-CDS-M-pyrifera_M_contig100512:387..4611622815501.4486244-CDS-M-pyrifera_M_contig100512:387..461Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100512 388..461 +
1692277439.9902341-CDS-M-pyrifera_M_contig100512:387..4611692277439.9902341-CDS-M-pyrifera_M_contig100512:387..461Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100512 388..461 +
1622815501.465559-CDS-M-pyrifera_M_contig100512:538..6641622815501.465559-CDS-M-pyrifera_M_contig100512:538..664Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100512 539..664 +
1692277439.9985342-CDS-M-pyrifera_M_contig100512:538..6641692277439.9985342-CDS-M-pyrifera_M_contig100512:538..664Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100512 539..664 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100512.107.1

>prot_M-pyrifera_M_contig100512.107.1 ID=prot_M-pyrifera_M_contig100512.107.1|Name=mRNA_M-pyrifera_M_contig100512.107.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=141bp
MALPPAHQSHRLGSAGAPVVIEVFWDLCCPFSTKSFRTFTKELMPWLEEE
HKGVVQVIFRQQIQAWHPQATMMHEAALAVEAVAGAKFWDYCAALFEEQE
AFFDPACMDLSRNQCYAQLADLAAKVGVSKEAVLEKLKVI*
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mRNA from alignment at M-pyrifera_M_contig100512:19..664+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100512.107.1 ID=mRNA_M-pyrifera_M_contig100512.107.1|Name=mRNA_M-pyrifera_M_contig100512.107.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=646bp|location=Sequence derived from alignment at M-pyrifera_M_contig100512:19..664+ (Macrocystis pyrifera P11B4 male)
GAGATTAGAATGGCTCTTCCACCTGCACATCAGTCACACCGCTTGGGCAG TGCGGGAGCCCCGGTTGTCATTGAGGTGTTCTGGGACCTCTGCTGTCCCT TCTCTACCAAGTCTTTCCGCACCTTCACGAAGGAGCTCATGCCTTGGCTC GAGGAGGAGCACAAGGGAGTAGTGCAGGTGATCTTCCGCCAGCAGATCCA GGCCTGGCATCCACAGGCGACGATGATGCATGGTGCGCCGCTCACTGCAG CCGCACTGCGGCAGCCCTCGCGATGCTGAGGGCTGCGCCGGCAGCTATTT GACCTGTGGCTGCTGGTCGCGTGCCTGGCACGGTAGTGAGGTGGCCTGGG CTGACGTTTCGTGAAACAGAGGCCGCACTCGCTGTTGAGGCGGTGGCGGG CGCCAAGTTCTGGGATTACTGCGCTGCGCTCTTCGAAGAGCAAGTGAGTC ACATAGACATGATTGCGTTCTCTCCTCGGGGACTTGAAGGCCCGCTGAAG AGTTCTGACGGCGGCTGCAGGAGGCCTTCTTCGACCCCGCCTGCATGGAC CTCTCGCGCAACCAGTGCTACGCCCAGCTGGCCGACCTTGCTGCCAAGGT GGGCGTGTCCAAGGAGGCAGTGCTCGAGAAGCTTAAGGTGATCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100512:19..664+

>mRNA_M-pyrifera_M_contig100512.107.1 ID=mRNA_M-pyrifera_M_contig100512.107.1|Name=mRNA_M-pyrifera_M_contig100512.107.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=846bp|location=Sequence derived from alignment at M-pyrifera_M_contig100512:19..664+ (Macrocystis pyrifera P11B4 male)
ATGGCTCTTCCACCTGCACATCAGTCACACCGCTTGGGCAGTGCGGGAGC
CCCGGTTGTCATTGAGGTGTTCTGGGACCTCTGCTGTCCCTTCTCTACCA
AGTCTTTCCGCACCTTCACGAAGGAGCTCATGCCTTGGCTCGAGGAGGAG
CACAAGGGAGTAGTGCAGGTGATCTTCCGCCAGCAGATCCAGGCCTGGCA
TCCACAGGCGACGATGATGCATGATGGCTCTTCCACCTGCACATCAGTCA
CACCGCTTGGGCAGTGCGGGAGCCCCGGTTGTCATTGAGGTGTTCTGGGA
CCTCTGCTGTCCCTTCTCTACCAAGTCTTTCCGCACCTTCACGAAGGAGC
TCATGCCTTGGCTCGAGGAGGAGCACAAGGGAGTAGTGCAGGTGATCTTC
CGCCAGCAGATCCAGGCCTGGCATCCACAGGCGACGATGATGCATGAGGC
CGCACTCGCTGTTGAGGCGGTGGCGGGCGCCAAGTTCTGGGATTACTGCG
CTGCGCTCTTCGAAGAGCAAAGGCCGCACTCGCTGTTGAGGCGGTGGCGG
GCGCCAAGTTCTGGGATTACTGCGCTGCGCTCTTCGAAGAGCAAGAGGCC
TTCTTCGACCCCGCCTGCATGGACCTCTCGCGCAACCAGTGCTACGCCCA
GCTGGCCGACCTTGCTGCCAAGGTGGGCGTGTCCAAGGAGGCAGTGCTCG
AGAAGCTTAAGGTGATCTGAGAGGCCTTCTTCGACCCCGCCTGCATGGAC
CTCTCGCGCAACCAGTGCTACGCCCAGCTGGCCGACCTTGCTGCCAAGGT
GGGCGTGTCCAAGGAGGCAGTGCTCGAGAAGCTTAAGGTGATCTGA
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