mRNA_M-pyrifera_M_contig10027.59.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig10027.59.1
Unique NamemRNA_M-pyrifera_M_contig10027.59.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: D7FQ44_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQ44_ECTSI)

HSP 1 Score: 182 bits (462), Expect = 1.570e-57
Identity = 83/92 (90.22%), Postives = 88/92 (95.65%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGIAARG EILA+GEGDD VTNT+QLIGKFL+LRGPDK DHEVDSVEHCDKFWYWLQ+KGINSNRSGIVNAIAEKVNTWIPGIYDADSY +
Sbjct:   33 VPGIAARGAEILADGEGDDKVTNTYQLIGKFLALRGPDKTDHEVDSVEHCDKFWYWLQAKGINSNRSGIVNAIAEKVNTWIPGIYDADSYTD 124
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: K0QZN8_THAOC (Uncharacterized protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0QZN8_THAOC)

HSP 1 Score: 133 bits (334), Expect = 5.360e-38
Identity = 58/90 (64.44%), Postives = 73/90 (81.11%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSY 272
            VPGI     + LAEGE D+C+TNT+QLIGKFL L+GPD DD++V+S+EHC+KFWYWLQ K I+++RS IV AIA KVN  +PG+YDAD Y
Sbjct:   33 VPGIGPAAAKKLAEGEHDECITNTYQLIGKFLMLKGPDTDDNKVESMEHCEKFWYWLQDKQISAHRSAIVKAIAMKVNGLLPGVYDADLY 122
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A835Z4N7_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4N7_9STRA)

HSP 1 Score: 127 bits (320), Expect = 6.400e-36
Identity = 57/90 (63.33%), Postives = 67/90 (74.44%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSY 272
            VPGI     + LA GEG + V NT+QLIGKFLSL+G D     + ++EHCD+FWYWL+  GI+S RSGIVNAIAEK NTWIPGIYD D Y
Sbjct:   34 VPGIGPAAVKKLAAGEGSNKVANTYQLIGKFLSLKGGDDSGKTIQTIEHCDRFWYWLKEMGISSYRSGIVNAIAEKCNTWIPGIYDEDQY 123
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A7S2R0A3_9STRA (Hypothetical protein n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2R0A3_9STRA)

HSP 1 Score: 125 bits (314), Expect = 7.030e-35
Identity = 58/93 (62.37%), Postives = 70/93 (75.27%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGD-DCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGI    K  LA G+ + D +TNT QLIGKFL L+GPD+DD ++D +EH +KFWYWL+ KGI S+RS IV AI EK+NT IPGIYDA  YAE
Sbjct:   34 VPGIGPAAKRALAGGDDESDRITNTFQLIGKFLMLKGPDEDDMKIDCMEHMEKFWYWLKQKGIKSHRSAIVKAIGEKINTMIPGIYDASIYAE 126
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A7S2V4V0_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V4V0_9STRA)

HSP 1 Score: 124 bits (310), Expect = 2.770e-34
Identity = 54/92 (58.70%), Postives = 72/92 (78.26%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGI       LA  + D+ +TN+ QLIGKFL ++GPD ++ EVD V HC+KFWY+L+SKG+NS+RSGIV+AIAEK+NT IPGIYD+D + +
Sbjct:   32 VPGIGKAAAAALASADDDEPITNSFQLIGKFLLMKGPDTEEGEVDCVMHCNKFWYYLKSKGVNSHRSGIVHAIAEKINTMIPGIYDSDVFED 123
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: B8CF00_THAPS (Uncharacterized protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CF00_THAPS)

HSP 1 Score: 122 bits (306), Expect = 6.020e-34
Identity = 53/92 (57.61%), Postives = 71/92 (77.17%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGI     + LA  EG++ +TNT+QLIGKFL L+GPD D ++V+S EHC+KFWYWLQ+ GI+++RS IV AIA+KVN  +PG+YD D Y +
Sbjct:   16 VPGIGPAAAKKLANVEGEEGITNTYQLIGKFLMLKGPDTDTNKVESFEHCEKFWYWLQAMGISAHRSAIVKAIAQKVNGLLPGVYDPDLYED 107
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A6U3Z2B1_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6U3Z2B1_9STRA)

HSP 1 Score: 120 bits (300), Expect = 8.880e-33
Identity = 59/96 (61.46%), Postives = 76/96 (79.17%), Query Frame = 3 
Query:    3 VPGIA-ARGKEILAEGEGD---DCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGI  A  K++   G+G+   D VTNT+QLIGKFL L+GPD+D ++V+SVEH +KFW++L+ KGI+S+RSGIV AIAEKVNT +PGIYDA  Y E
Sbjct:   32 VPGIGPAAAKKLGQIGDGETEFDRVTNTYQLIGKFLMLKGPDEDGNKVESVEHMEKFWWYLKQKGISSHRSGIVRAIAEKVNTMLPGIYDASEYEE 127
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A7S2MQ62_9STRA (Hypothetical protein n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2MQ62_9STRA)

HSP 1 Score: 120 bits (300), Expect = 1.220e-32
Identity = 61/96 (63.54%), Postives = 75/96 (78.12%), Query Frame = 3 
Query:    3 VPGI--AARGK--EILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGI  AA  K  EI  E +  D +TNT+QLIGKFL L+GPD+D H+V+S+EH +KFW++L+ KGI+S+RSGIV AIAEKVNT IPGIYDA  Y E
Sbjct:   41 VPGIGPAAARKLGEIGDEEKDSDRITNTYQLIGKFLMLKGPDEDGHKVESMEHMEKFWWFLKEKGISSHRSGIVRAIAEKVNTMIPGIYDASEYDE 136
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A7S1ZJR7_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZJR7_TRICV)

HSP 1 Score: 119 bits (298), Expect = 2.930e-32
Identity = 55/90 (61.11%), Postives = 66/90 (73.33%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEGDDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSY 272
            VPGI     + LAEG+ DD VTNT QLIGKF+ L+GPD D+H+V   EH  KFW+WL  K I S+RS IV AIAEK+N  +PGIYDAD+Y
Sbjct:   54 VPGIGPAAAKRLAEGDDDDRVTNTWQLIGKFMMLKGPDDDEHKVGPGEHMQKFWHWLAEKEIKSHRSAIVKAIAEKMNQMMPGIYDADAY 143
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BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Match: A0A7S2UIU1_9STRA (Hypothetical protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2UIU1_9STRA)

HSP 1 Score: 113 bits (283), Expect = 3.290e-30
Identity = 52/93 (55.91%), Postives = 71/93 (76.34%), Query Frame = 3 
Query:    3 VPGIAARGKEILAEGEG-DDCVTNTHQLIGKFLSLRGPDKDDHEVDSVEHCDKFWYWLQSKGINSNRSGIVNAIAEKVNTWIPGIYDADSYAE 278
            VPGI  +  E LA  +  DD +TNT+QLIGKFL L+GPD D+++V S++H +KFWY+L+ +GI S+RS IV AIAEK+N +IPGIY+ D Y +
Sbjct:   33 VPGIGPKAAEKLAASDDTDDRITNTYQLIGKFLMLKGPDDDENKVASMQHMEKFWYYLKERGIASHRSAIVKAIAEKMNMFIPGIYERDCYGD 125
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig10027.59.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FQ44_ECTSI1.570e-5790.22Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
K0QZN8_THAOC5.360e-3864.44Uncharacterized protein n=1 Tax=Thalassiosira ocea... [more]
A0A835Z4N7_9STRA6.400e-3663.33Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A7S2R0A3_9STRA7.030e-3562.37Hypothetical protein n=1 Tax=Eucampia antarctica T... [more]
A0A7S2V4V0_9STRA2.770e-3458.70Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
B8CF00_THAPS6.020e-3457.61Uncharacterized protein n=1 Tax=Thalassiosira pseu... [more]
A0A6U3Z2B1_9STRA8.880e-3361.46Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
A0A7S2MQ62_9STRA1.220e-3263.54Hypothetical protein n=1 Tax=Helicotheca tamesis T... [more]
A0A7S1ZJR7_TRICV2.930e-3261.11Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
A0A7S2UIU1_9STRA3.290e-3055.91Hypothetical protein n=1 Tax=Attheya septentrional... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig10027contigM-pyrifera_M_contig10027:575..3329 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons3
Model size328
Cds size327
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig10027.59.1prot_M-pyrifera_M_contig10027.59.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig10027 575..3328 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815497.8967068-CDS-M-pyrifera_M_contig10027:574..7051622815497.8967068-CDS-M-pyrifera_M_contig10027:574..705Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10027 575..705 +
1692277438.7258298-CDS-M-pyrifera_M_contig10027:574..7051692277438.7258298-CDS-M-pyrifera_M_contig10027:574..705Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10027 575..705 +
1622815497.9089136-CDS-M-pyrifera_M_contig10027:2224..23421622815497.9089136-CDS-M-pyrifera_M_contig10027:2224..2342Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10027 2225..2342 +
1692277438.7355633-CDS-M-pyrifera_M_contig10027:2224..23421692277438.7355633-CDS-M-pyrifera_M_contig10027:2224..2342Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10027 2225..2342 +
1622815497.9236705-CDS-M-pyrifera_M_contig10027:3250..33281622815497.9236705-CDS-M-pyrifera_M_contig10027:3250..3328Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10027 3251..3328 +
1692277438.7478201-CDS-M-pyrifera_M_contig10027:3250..33281692277438.7478201-CDS-M-pyrifera_M_contig10027:3250..3328Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10027 3251..3328 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig10027.59.1

>prot_M-pyrifera_M_contig10027.59.1 ID=prot_M-pyrifera_M_contig10027.59.1|Name=mRNA_M-pyrifera_M_contig10027.59.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=109bp
RCPASRLEGRRSWQRERETTASPTHTSSSESSCLSAAPTRTITRWTPWST
ATNSGTGCSPRASTPTDPESLTRSPRRLTRGFLVSTTLTATPSNDDSSTV
QTGGPYSVP
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mRNA from alignment at M-pyrifera_M_contig10027:575..3329+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig10027.59.1 ID=mRNA_M-pyrifera_M_contig10027.59.1|Name=mRNA_M-pyrifera_M_contig10027.59.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2755bp|location=Sequence derived from alignment at M-pyrifera_M_contig10027:575..3329+ (Macrocystis pyrifera P11B4 male)
AGGTGCCCGGCATCGCGGCTAGAGGGAAGGAGATCCTGGCAGAGGGAGAG
GGAGACGACTGCGTCACCAACACACACCAGCTCATCGGAAAGTTCTTGTC
TCTCCGCGGCCCCGACAAGGACGATCACGAGGTGCGGGAAGAAAAAGAGA
GACAGCGGGGAGACCCGTGCTATGGGAGGGGGTTCACTTGATAGGATACA
AGGAGCCGATAGTATACAAGGAGCCCAAACTTTTCTCACTGACGCAGCAG
CTCCTCAACCGTTGTAGCTGAGGAGCAGCTACCCATTGTACTGTTGGATG
CGATGAATACCCGGTATGTTTTTTACACATTAGTCGTTGGGCACCTCGCC
TATGGGATTGCGTGTCGGGCTTTAGGTTTTTTGCTGTCATTCGCGGCCGA
AGAGCGCCGGCGGATCTCGGTCCCGACACCGATCACCGAAGGGCTTGCGC
CGTTCGCCTTTCATGCGTGCGTTCATGGTTGTCTCTCAATCCCCGTTCGA
CTACTACAGTAGGTATCCCAACACGAAAGTGGTTCGGCATAGCGGGGATA
GACGCGAATAAGTCGACTCTCAACGTTGAAACCCGGAAAAATCTCCACCG
GTCAGCCCGTGGGGGAATCTGTCCCCCTAAACGTTTACAATCAAATATTT
TTTAGCTTGTGGCTGTAGAAGAACGCCTCATCACCCTGCTCGAGGAGTTT
TAGGTAGGTTGTACTGTTTTGTCAACCCGGATGTTGGGGCCCCTGACTGG
TGCTACGGTAGACGTAGCACTTGTTTTTTCTGAGGCTGCTCACTTGATTA
TAGTGTGGGAACTCTATGCCCCCTCCCAAAACTGATTTTGGGCGAAGCAA
GAAGTGCTTCAGCGATGGACTACTGCTGTAGGCGTATCACGAATAATATT
GTAAAAACACTGACGGTATTCCAACGATGTTGGGTTGCCCCTTTTTGTGC
TGCGGTCCTGTTTTGGGGAGAAAGCACTCAAGTTCCAAGTGTTTTGTCCC
CAAAACGGAACCGCAGCGCTAAAAGGGTCAAGGAATCCGGTCGGTCTGAA
ATATGTCGGCCGAAAAGCGCTACCCGTATATAGCAGCACGCCTCCCCCCA
AACGGGGAGACGCCTCGGAGAATATAGACGCTAGCCCCATCGTAGAACGG
ATCAAGAGGGGAAGGCGGGCATCGTCTTTTGGCATTACACGAAAAGAAGT
TCTCGGTCGTATATATCTAGTACGGTCTGTTCTCGTGCCGAAGTTGAGGT
TCATGCGCACAAAAAAAAAATGTTTCGCTTGAGAGTTTGTTGTAAAACCA
GAGGCTTTTTTTTTTGCGGTATTTTTCGATAGTTGAACCGCACGCACACG
TGCCCCACGGCTAGTTTCGGCCCTCGCGCGCGTCTGGTTTTGTTGTCGCT
CTCGGGGCGTACGTTGCGGTGCCACCATTCGTGCACAGCGCTTACTGCGG
TAAGACGTTGCAACTCCTGTTTTAGGGCTGCTGGCGTGAATACCTATGTA
CCCATCACCGACCATTTCTAGCGATCGTTTGAGTATGTAACTGCCTACCG
TGGTGAAGGTTTCTAACGTCCCGGCGTTTTTCTTTGGGGGGGTGGATGGG
GCGTGCCGGTTTTTCTTTCGTGCCTGTCTGTCTACGGCTACATTTCGAAG
GTGGACTCCGTGGAGCACTGCGACAAATTCTGGTACTGGTTGCAGTCCAA
GGGCATCAACTCCAACCGATCCGGAATCGTTAACGCGATCGCCGAGAAGG
TTAACACGTGGATTCCTGGTGAGGAGGATCCCTCGGAAGGATAATGCTTG
TTTTGATATTACGGGGGACCATAGTAAATAGGACCAAATATTTTTAGTAA
AGAATAGGTAACTATATAGGTTTTTGTGTGTACCGTAAGTCCTATTTACT
ATGGTCCCCTGTAATAGCATGTAATTCCGGCAGGCGTAACGGTAGTGGGA
CGGCAATGCTGCCCTTGGATGGCGTTTCGCTATCGTAGGACATCGTTCCG
AGTGAATATCATCACGAAGCGTCGTGGGCATGGAGCGGACAGGAGATACG
GCCGTGAGTCTGAGGAACCGCGTGCGCGCCGGCCGTTGAAAACCGGACAA
TATTGCAGTGGAACCAGAACCTCGGCCGTTGACGTGAAGTGCACTGAAGT
GAGGATTGGAATCCGCTTCGTAGCCCGCAATGAAAGCGACACCAGAAGGT
GTTGACTGTTTTAACAAACGACTGGTATAGTTCCCGGGAAACCAATATTT
GAATTGAATGCTGATAATTACTCAAGACTCCCCTTCCTTGCTTGAACCAT
TGTCCGCTGTAGCAGCAGCAGTAGTGGCAGCAACAGTATGTACCATTGTC
TGCGGACGATATACAATTGCTCGGAAAACCTGTTGAGGTGTTGGATATTA
GCATTTTGTCGCCTCTGTGATGCATAGTTGAGAGATATTGCGGACGATGT
TTCGTCCTAGTGTCGTCCGGTTGGCGTAGAGTTGTGGCTCGCCAGCCGAC
CTCGCATCGGTGCGCTGTTCCCGTTGCCAGAGTAGGGCACACGCCCTATT
GGGCACGATGTTTCTTCTGCCGTGTGATGGCCGAAAGAGCTTTTTGTTCC
CTCCGACATGTCGCCCCTCCCCTCCCCTCCCCTGCCCCCCCTTCCCTTCC
TTGCCGCTTTCTCCTTTTTTTCCCAGGTATCTACGACGCTGACAGCTACG
CCGAGTAATGACGATTCAAGTACGGTCCAGACAGGCGGCCCATATAGTGT
TCCGG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig10027:575..3329+

>mRNA_M-pyrifera_M_contig10027.59.1 ID=mRNA_M-pyrifera_M_contig10027.59.1|Name=mRNA_M-pyrifera_M_contig10027.59.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=654bp|location=Sequence derived from alignment at M-pyrifera_M_contig10027:575..3329+ (Macrocystis pyrifera P11B4 male)
AGGTGCCCGGCATCGCGGCTAGAGGGAAGGAGATCCTGGCAGAGGGAGAG
GGAGACGACTGCGTCACCAACACACACCAGCTCATCGGAAAGTTCTTGTC
TCTCCGCGGCCCCGACAAGGACGATCACGAGAGGTGCCCGGCATCGCGGC
TAGAGGGAAGGAGATCCTGGCAGAGGGAGAGGGAGACGACTGCGTCACCA
ACACACACCAGCTCATCGGAAAGTTCTTGTCTCTCCGCGGCCCCGACAAG
GACGATCACGAGGTGGACTCCGTGGAGCACTGCGACAAATTCTGGTACTG
GTTGCAGTCCAAGGGCATCAACTCCAACCGATCCGGAATCGTTAACGCGA
TCGCCGAGAAGGTTAACACGTGGATTCCTGGTGGACTCCGTGGAGCACTG
CGACAAATTCTGGTACTGGTTGCAGTCCAAGGGCATCAACTCCAACCGAT
CCGGAATCGTTAACGCGATCGCCGAGAAGGTTAACACGTGGATTCCTGGT
ATCTACGACGCTGACAGCTACGCCGAGTAATGACGATTCAAGTACGGTCC
AGACAGGCGGCCCATATAGTGTTCCGGTATCTACGACGCTGACAGCTACG
CCGAGTAATGACGATTCAAGTACGGTCCAGACAGGCGGCCCATATAGTGT
TCCG
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