mRNA_M-pyrifera_M_contig100217.49.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100217.49.1
Unique NamemRNA_M-pyrifera_M_contig100217.49.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A353ZHA4_9PLAN (GTP-binding protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A353ZHA4_9PLAN)

HSP 1 Score: 201 bits (510), Expect = 4.760e-61
Identity = 97/168 (57.74%), Postives = 130/168 (77.38%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDDAPEQT  V+ Q+ESRKT     +GF EDD S VS++TL+VVTRGDDP  A+R+AFF+EM P  +   + ++ + PE++ +LR ++YE +DVIR YTK+PGKPA+   PFT+P  A+VEDLA +VH+DLAE L HA++WG+   DGQ+VGR+H+LAD D+VELH
Sbjct:  162 GSSDDAPEQTQQVIEQIESRKTLLGKHTGFAEDDFSRVSIRTLLVVTRGDDPGCADRLAFFREMYPR-EFDTLLVECDRPESLAALRTRIYELLDVIRIYTKQPGKPADRTSPFTVPRNATVEDLAGRVHKDLAERLTHARVWGSGVHDGQSVGRDHVLADCDLVELH 328          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A386C7F4_9BACT (Putative GTPase n=1 Tax=uncultured bacterium TaxID=77133 RepID=A0A386C7F4_9BACT)

HSP 1 Score: 197 bits (501), Expect = 1.140e-59
Identity = 96/170 (56.47%), Postives = 129/170 (75.88%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTAT-QDGQTVGREHILADRDMVELHT 507
            GSSDDAPEQT  V+ Q+E RKT  +  SGF +D+ S++ VKTL+VVT GDDP   +R+AFF EM P        ++ + P++V+ LRN+++  + VIRAYTK PGKPA+Y  PFT+P G+ VEDLA KVH+DLA+++K+AKIWGT+    GQ+VGR+HILAD+D+VELHT
Sbjct:  162 GSSDDAPEQTVTVIKQMEERKTMLAVESGFVDDNFSLIRVKTLLVVTHGDDPGRDDRLAFFHEMLPSQSFQTQMVEFDRPDSVQELRNRIFALLGVIRAYTKAPGKPADYTSPFTVPIGSMVEDLAGKVHKDLADTVKYAKIWGTSVAHGGQSVGRDHILADKDLVELHT 331          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A3E0QA29_9BACT (TGS domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3E0QA29_9BACT)

HSP 1 Score: 191 bits (485), Expect = 2.760e-57
Identity = 95/168 (56.55%), Postives = 122/168 (72.62%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDD  E TADV+ QL  RKT   + SGFDE+D SI  V T +VVTRG DPD   R+   +E+  +I  +   ++L+  E+VE LR  +Y+++ VIR YTKKPGKPA+Y  PFT+P G +VEDLA KVH+DL + LK+AK+WG +  DGQTVGREH+LADRD+VELH
Sbjct:  162 GSSDDGVEHTADVIEQLRQRKTILDSRSGFDEEDYSITHVLTFLVVTRGGDPDVETRLELLRELA-EIPFTTHVVELDRHESVEGLRRSIYDALGVIRVYTKKPGKPADYEAPFTIPVGGTVEDLAGKVHRDLLDGLKYAKVWGESAHDGQTVGREHVLADRDLVELH 328          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: UPI00142266F5 (TGS domain-containing protein n=1 Tax=Rubinisphaera sp. JC658 TaxID=2713176 RepID=UPI00142266F5)

HSP 1 Score: 186 bits (473), Expect = 1.760e-55
Identity = 92/168 (54.76%), Postives = 123/168 (73.21%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDDAP++TA++L Q  SR T     +GF +DD S+V VKTL+VVTR       ER+ +F+EM P     +  ++ +  ++ E LR++V++ + VIR YTK PGKPAEY DPFT+P G +VEDLA+KVH+DLAESLK AK+WG +  DGQ+VGR+H LADRD+VELH
Sbjct:  162 GSSDDAPDETAELLEQFRSRSTMLGRENGFVDDDFSLVQVKTLVVVTRAGSDGLEERLEYFREMVP-TPFEIQRVEFDREDSREELRDRVFQMLGVIRVYTKAPGKPAEYKDPFTIPVGGTVEDLAVKVHRDLAESLKFAKVWGESAHDGQSVGRDHQLADRDLVELH 328          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A2E7NR69_9PLAN (GTP-binding protein n=2 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2E7NR69_9PLAN)

HSP 1 Score: 184 bits (466), Expect = 1.990e-54
Identity = 89/168 (52.98%), Postives = 121/168 (72.02%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDDAP+ T  V+ Q   RKT FS  +GFDEDD S + V+TL+V TR  DPDA  R+AF +E+    +   V ++L+ P++  SLR  VY  +DVIR YTK+PGK A++  PFT+P  A+VEDLA++VH++LA + K A+IWG    DGQ+VGREH+L+D+D+VELH
Sbjct:  162 GSSDDAPDDTEAVMRQFSERKTLFSNRTGFDEDDFSTIHVQTLLVATRASDPDAETRIAFLQELTGS-EFETVRVELDNPDSCNSLREIVYRKLDVIRVYTKRPGKTADFESPFTVPRNATVEDLALRVHRELATTFKFARIWGDTVHDGQSVGREHVLSDKDLVELH 328          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A2E8FJ97_9PLAN (GTP-binding protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2E8FJ97_9PLAN)

HSP 1 Score: 182 bits (463), Expect = 5.770e-54
Identity = 84/168 (50.00%), Postives = 121/168 (72.02%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDDAP+ T  V++Q   RKT     +GFDEDD SIV+++TL+V TR DDP   +R+ +  E        V+ + L+ PE+ E+LR+ +Y ++DV+R YTK+PGKPA++  PFT+P   ++EDLA++VH+DLA  LK A+IWG    DGQ+VGR+H+L+D+D+VELH
Sbjct:  162 GSSDDAPDDTLTVVSQFRDRKTHLCDHTGFDEDDFSIVNLQTLLVATRADDPACGDRLEYLAEAGDLTGFQVIRVALDDPESREALRDAIYHTLDVVRVYTKRPGKPADFEAPFTVPRNGTIEDLALQVHRDLARDLKFARIWGQGVHDGQSVGRDHVLSDKDLVELH 329          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A517Z8C9_9PLAN (GTPase Obg n=1 Tax=Maioricimonas rarisocia TaxID=2528026 RepID=A0A517Z8C9_9PLAN)

HSP 1 Score: 181 bits (459), Expect = 2.240e-53
Identity = 93/168 (55.36%), Postives = 122/168 (72.62%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDDAP++TA VL Q  +RKT     SGFDEDD S+V V T ++VTRGDDPD A R+  F+E+   +  +V  ++LE  E+VESLR  +Y+++ V R YTK+PGKPA+Y  PFT+P G  VEDLA K+H++LA+SLK A++W     DGQTVGR+  L DRD+VELH
Sbjct:  162 GSSDDAPDETAAVLEQFAARKTVLDAHSGFDEDDYSVVHVNTRLLVTRGDDPDVATRLELFREVVA-LPFAVDRVELERAESVESLRRTIYDALGVARIYTKRPGKPADYDAPFTIPRGGLVEDLAGKIHRELADSLKFARVWRDNELDGQTVGRDFPLCDRDVVELH 328          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A7C4QSG4_9PLAN (TGS domain-containing protein n=1 Tax=Schlesneria paludicola TaxID=360056 RepID=A0A7C4QSG4_9PLAN)

HSP 1 Score: 179 bits (453), Expect = 1.830e-52
Identity = 90/168 (53.57%), Postives = 118/168 (70.24%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDDAPEQT +V+ Q   R T  S  +GFD DD S + VKTL+VVTRG DPDAA R  F +E+ P I     T++ +   + E+LR Q++ S DVIR YTK PGKPA++  PF +P G +VEDLA K+H+DLAE +K AK+W   + D + VGR+H+L+DRD+VELH
Sbjct:  163 GSSDDAPEQTVEVIQQFARRHTFLSDHTGFDPDDFSQLHVKTLLVVTRGSDPDAAARADFLRELLP-IPFPGTTVECDDAPSREALREQIFRSADVIRIYTKTPGKPADFSAPFCIPRGGTVEDLAAKIHRDLAEKVKSAKLWAHGSSDARIVGRDHVLSDRDLVELH 329          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A2E0SK81_9PLAN (GTP-binding protein (Fragment) n=1 Tax=Planctomyces sp. TaxID=37635 RepID=A0A2E0SK81_9PLAN)

HSP 1 Score: 174 bits (442), Expect = 2.290e-52
Identity = 84/168 (50.00%), Postives = 121/168 (72.02%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDD+PE T ++L QL  RKT  ++ SGF EDD S + ++TL VVTRG DP+A+ R+ F +EM  ++    + +DL+  E  E LR +++E +  +R YTK PGKPA+Y  PFT+P G  VEDLA  +H++L +++K AK+WG + +DGQTVGR+H+L D+D+VELH
Sbjct:   38 GSSDDSPEATVELLEQLAQRKTVLASESGFVEDDFSRIQIRTLFVVTRGRDPEASMRVDFLREMH-ELPFEPLYVDLDNSEDCELLRGKIFELLHCMRIYTKAPGKPADYSSPFTIPRGGKVEDLAYVIHRELFDTMKFAKVWGESARDGQTVGRDHVLCDKDLVELH 204          
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Match: A0A518CH87_9PLAN (GTPase Obg n=1 Tax=Polystyrenella longa TaxID=2528007 RepID=A0A518CH87_9PLAN)

HSP 1 Score: 178 bits (452), Expect = 2.450e-52
Identity = 90/168 (53.57%), Postives = 119/168 (70.83%), Query Frame = 1
Query:    1 GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGDDPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAYTKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDGQTVGREHILADRDMVELH 504
            GSSDD+PE T ++L QLE RKT  ++ SGF EDD S + +KTL VVTRG + +A  R+ F +EMR D     + +DLE     E LR Q+++ +D IR YTK PGKPA+Y  PFT+P G SVEDLA  +H++L +S+K AK+WG +  DGQTVGR+H L D+D+VELH
Sbjct:  162 GSSDDSPEATVELLQQLEQRKTVLASQSGFVEDDFSRIQIKTLFVVTRGKNAEARMRVEFLREMR-DHPFEPLYVDLENEADQEELRTQIFQLLDCIRIYTKAPGKPADYSSPFTIPRGGSVEDLAYVIHRELFDSMKFAKVWGESAHDGQTVGRDHQLCDKDLVELH 328          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100217.49.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A353ZHA4_9PLAN4.760e-6157.74GTP-binding protein n=1 Tax=Planctomycetaceae bact... [more]
A0A386C7F4_9BACT1.140e-5956.47Putative GTPase n=1 Tax=uncultured bacterium TaxID... [more]
A0A3E0QA29_9BACT2.760e-5756.55TGS domain-containing protein n=1 Tax=Planctomycet... [more]
UPI00142266F51.760e-5554.76TGS domain-containing protein n=1 Tax=Rubinisphaer... [more]
A0A2E7NR69_9PLAN1.990e-5452.98GTP-binding protein n=2 Tax=Planctomycetaceae bact... [more]
A0A2E8FJ97_9PLAN5.770e-5450.00GTP-binding protein n=1 Tax=Planctomycetaceae bact... [more]
A0A517Z8C9_9PLAN2.240e-5355.36GTPase Obg n=1 Tax=Maioricimonas rarisocia TaxID=2... [more]
A0A7C4QSG4_9PLAN1.830e-5253.57TGS domain-containing protein n=1 Tax=Schlesneria ... [more]
A0A2E0SK81_9PLAN2.290e-5250.00GTP-binding protein (Fragment) n=1 Tax=Planctomyce... [more]
A0A518CH87_9PLAN2.450e-5253.57GTPase Obg n=1 Tax=Polystyrenella longa TaxID=2528... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100217contigM-pyrifera_M_contig100217:171..680 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score135.6
Seed ortholog evalue2.2e-29
Seed eggNOG ortholog1210884.HG799466_gene12845
KEGG koko:K06944
Hectar predicted targeting categoryother localisation
EggNOG free text desc.TGS domain
EggNOG OGs2IWYP@203682,COG1163@1,COG1163@2
COG Functional cat.S
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons1
Model size510
Cds size510
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815497.472675-CDS-M-pyrifera_M_contig100217:170..6801622815497.472675-CDS-M-pyrifera_M_contig100217:170..680Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100217 171..680 -
1692277438.5362682-CDS-M-pyrifera_M_contig100217:170..6801692277438.5362682-CDS-M-pyrifera_M_contig100217:170..680Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100217 171..680 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100217.49.1prot_M-pyrifera_M_contig100217.49.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100217 171..680 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100217.49.1

>prot_M-pyrifera_M_contig100217.49.1 ID=prot_M-pyrifera_M_contig100217.49.1|Name=mRNA_M-pyrifera_M_contig100217.49.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
GSSDDAPEQTADVLTQLESRKTRFSTWSGFDEDDMSIVSVKTLMVVTRGD
DPDAAERMAFFKEMRPDIDLSVVTIDLECPEAVESLRNQVYESIDVIRAY
TKKPGKPAEYVDPFTLPAGASVEDLAIKVHQDLAESLKHAKIWGTATQDG
QTVGREHILADRDMVELHT*
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mRNA from alignment at M-pyrifera_M_contig100217:171..680-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100217.49.1 ID=mRNA_M-pyrifera_M_contig100217.49.1|Name=mRNA_M-pyrifera_M_contig100217.49.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig100217:171..680- (Macrocystis pyrifera P11B4 male)
GGGAGCAGCGATGACGCCCCGGAGCAGACGGCGGATGTGCTGACCCAGTT GGAGAGTCGGAAGACACGATTTTCGACGTGGTCGGGATTCGACGAAGACG ACATGTCGATTGTCTCTGTGAAGACGCTGATGGTCGTCACCCGTGGCGAT GATCCTGATGCTGCAGAGCGAATGGCATTCTTCAAGGAGATGCGACCGGA CATCGACCTGTCAGTCGTCACCATCGATTTGGAATGTCCTGAAGCGGTGG AATCGCTGAGGAATCAAGTCTACGAGTCGATTGATGTCATCAGGGCATAC ACAAAGAAACCTGGAAAACCGGCGGAGTATGTTGATCCCTTCACCCTGCC GGCGGGGGCGAGCGTGGAGGACCTTGCCATCAAGGTGCATCAAGACCTTG CCGAATCGCTCAAACACGCAAAGATATGGGGGACTGCGACACAGGACGGG CAAACCGTCGGTCGCGAACACATTCTCGCCGACCGCGACATGGTCGAATT GCACACTTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100217:171..680-

>mRNA_M-pyrifera_M_contig100217.49.1 ID=mRNA_M-pyrifera_M_contig100217.49.1|Name=mRNA_M-pyrifera_M_contig100217.49.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig100217:171..680- (Macrocystis pyrifera P11B4 male)
GGGAGCAGCGATGACGCCCCGGAGCAGACGGCGGATGTGCTGACCCAGTT
GGAGAGTCGGAAGACACGATTTTCGACGTGGTCGGGATTCGACGAAGACG
ACATGTCGATTGTCTCTGTGAAGACGCTGATGGTCGTCACCCGTGGCGAT
GATCCTGATGCTGCAGAGCGAATGGCATTCTTCAAGGAGATGCGACCGGA
CATCGACCTGTCAGTCGTCACCATCGATTTGGAATGTCCTGAAGCGGTGG
AATCGCTGAGGAATCAAGTCTACGAGTCGATTGATGTCATCAGGGCATAC
ACAAAGAAACCTGGAAAACCGGCGGAGTATGTTGATCCCTTCACCCTGCC
GGCGGGGGCGAGCGTGGAGGACCTTGCCATCAAGGTGCATCAAGACCTTG
CCGAATCGCTCAAACACGCAAAGATATGGGGGACTGCGACACAGGACGGG
CAAACCGTCGGTCGCGAACACATTCTCGCCGACCGCGACATGGTCGAATT
GCACACTTAGGGGAGCAGCGATGACGCCCCGGAGCAGACGGCGGATGTGC
TGACCCAGTTGGAGAGTCGGAAGACACGATTTTCGACGTGGTCGGGATTC
GACGAAGACGACATGTCGATTGTCTCTGTGAAGACGCTGATGGTCGTCAC
CCGTGGCGATGATCCTGATGCTGCAGAGCGAATGGCATTCTTCAAGGAGA
TGCGACCGGACATCGACCTGTCAGTCGTCACCATCGATTTGGAATGTCCT
GAAGCGGTGGAATCGCTGAGGAATCAAGTCTACGAGTCGATTGATGTCAT
CAGGGCATACACAAAGAAACCTGGAAAACCGGCGGAGTATGTTGATCCCT
TCACCCTGCCGGCGGGGGCGAGCGTGGAGGACCTTGCCATCAAGGTGCAT
CAAGACCTTGCCGAATCGCTCAAACACGCAAAGATATGGGGGACTGCGAC
ACAGGACGGGCAAACCGTCGGTCGCGAACACATTCTCGCCGACCGCGACA
TGGTCGAATTGCACACTTAG
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